workshop for priority-setting in aedes aegypti control interventions in latin america and the caribbean: a policy dialogue.this article presents the results of a dialogue between decision-makers and experts in latin america and the caribbean on priority-setting for interventions and studies on aedes aegypti control. the article is part of a project that included a systematic review of mosquito control strategies and a qualitative study with key informants from the region. using a collective deliberative process assisted by the results of the above-mentioned projects, a list of priorities was developed by consensus f ...201930994739
larvicidal activity of ocimum campechianum, ocotea quixos and piper aduncum essential oils against aedes aegypti.aedes aegypti, the main arbovirus vector of the yellow fever, dengue, chikungunya and zika viruses, is widely distributed in tropical and subtropical areas throughout the world. preventive control efforts have been implemented worldwide aimed at reducing its impact on human health. the recent reduction of chemicals available for vector control due to their negative impact on the environment and human health and the increase in mosquito resistance to insecticides have driven the research communit ...201930994444
downregulation of female doublesex expression by oral-mediated rna interference reduces number and fitness of anopheles gambiae adult females.mosquito-borne diseases affect millions worldwide, with malaria alone killing over 400 thousand people per year and affecting hundreds of millions. to date, the best strategy to prevent the disease remains insecticide-based mosquito control. however, insecticide resistance as well as economic and social factors reduce the effectiveness of the current methodologies. alternative control technologies are in development, including genetic control such as the sterile insect technique (sit). the sit i ...201930992032
gone in 60 seconds: sub-lethal effects of metofluthrin vapors on behavior and fitness of resistant and field strains of aedes aegypti (diptera: culicidae).spatial repellents can reduce fecundity and interrupt oviposition behavior in aedes aegypti. yet, it is unclear if short exposure times, resistant phenotypes, and other aspects of spatial repellents can impact these effects on mosquito reproduction. to address these issues, pyrethroid susceptible, pyrethroid resistant, and field strains of ae. aegypti were used to evaluate the extent to which fecundity and oviposition behavior are affected following metofluthrin exposure. mosquitoes were exposed ...201930989189
prevalence of kdr mutations and insecticide susceptibility among natural population of aedes aegypti in west bengal.aedes albopictus and aedes aegypti are the major vectors of arboviral diseases. as effective vaccines are not available for most of the arboviral diseases, vector control by using insecticides play the key role to reduce the disease transmission. the emergence and spread of resistance to different classes of insecticides by the vectors is a major obstacle to control the disease transmission. information about vector susceptibility to different insecticides and their mechanisms are very important ...201930986273
aedes aegypti hpx8c modulates immune responses against viral infection.mosquitoes act as vectors of numerous pathogens that cause human diseases. dengue virus (denv) transmitted by mosquito, aedes aegypti, is responsible for dengue fever epidemics worldwide with a serious impact on human health. currently, disease control mainly relies on vector targeted intervention strategies. therefore, it is imperative to understand the molecular mechanisms underlying the innate immune response of mosquitoes against pathogens. in the present study, the expression profiles of im ...201930986216
target and non-target botanical pesticides effect of trichodesma indicum (linn) r. br. and their chemical derivatives against the dengue vector, aedes aegypti l.the effects of crude ethanol derived leaf extract trichodesma indicum (linn) (ex-ti) and their chief derivatives were accessed on the survival and development of the dengue mosquito ae. aegypti also their non-toxic activity against mosquito predator. t. indicum is recognized to be the vital weed plant and a promising herb in the traditional ayurvedic medicine. in this study, the gc-ms chromatogram of ex-ti showed higher peak area percentage for cis-10-heptadecenoic acid (21.83%) followed by cycl ...201930977009
hsp superfamily of genes in the malaria vector anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance.heat shock proteins (hsps) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress. there is still no report regarding the diversity and phylogenetics research of hsp superfamily of genes at whole genome level in insects, and the hsp gene association with pyrethroid resistance is also not well known. the present study investigated the diversity, classification, scaffold location, characteristics, and phylogenetics of the superfamily ...201930975215
neem oil increases the persistence of the entomopathogenic fungus metarhizium anisopliae for the control of aedes aegypti (diptera: culicidae) larvae.the entomopathogenic fungus metarhizium anisopliae is a candidate for the integrated management of the disease vector mosquito aedes aegypti. metarhizium anisopliae is pathogenic and virulent against ae. aegypti larvae; however, its half-life is short without employing adjuvants. here, we investigated the use of neem oil to increase virulence and persistence of the fungus under laboratory and simulated field conditions.201930975207
a method for assessing mitochondrial physiology using mechanically permeabilized flight muscle of aedes aegypti mosquitoes.aedes aegypti is the most important and widespread vector of arboviruses, including dengue and zika. insect dispersal through the flight activity is a key parameter that determines vector competence, and is energetically driven by oxidative phosphorylation in flight muscle mitochondria. analysis of mitochondrial function is central for a better understanding of cellular metabolism, and is mostly studied using isolated organelles. however, this approach has several challenges and methods for asse ...201930974092
aedes aegypti nest1 protein enhances zika virus pathogenesis by activating neutrophils.saliva from the mosquito vector of flaviviruses is capable of changing the local immune environment, leading to an increase in flavivirus-susceptible cells at the infected bite site. in addition, an antibody response to specific salivary gland (sg) components changes the pathogenesis of flaviviruses in human populations. to investigate whether antigenic sg proteins are capable of enhancing infection with zika virus (zikv), a reemerging flavivirus primarily transmitted by the aedes aegypti mosqui ...201930971475
expanding the canon: non-classical mosquito genes at the interface of arboviral infection.mosquito transmitted viruses cause significant morbidity and mortality in human populations. despite the use of insecticides and other measures of vector control, arboviral diseases are on the rise. one potential solution for limiting disease transmission to humans is to render mosquitoes refractory to viral infection through genetic modification. substantial research effort in drosophila, aedes and anopheles has helped to define the major innate immune pathways, including toll, imd, jak/stat an ...201930970277
abiotic factors and population dynamic of aedes aegypti and aedes albopictus in an endemic area of dengue in brazil.aedes aegypti and aedes albopictus are exotic species in the americas with high epidemiological relevance as they are vectors of many pathogens. this study aimed at understanding the population dynamics of a. aegypti and a. albopictus and the influence of abiotic factors in an endemic area of dengue. the study was conducted in the urban area of campo grande, mato grosso do sul, brazil, over one year. in seven regions of the city, 50 ovitraps were installed in each neighborhood. the development o ...201930970109
evaluation of the larvicidal potential of the essential oil pogostemon cablin (blanco) benth in the control of aedes aegypti.the objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of pogostemon cablin leaves. the chemical characterization was performed by gas chromatography coupled to mass spectrometer (gc-ms). the larvicidal activity was performed according to the protocol of the world health organization. the antioxidant activity was ...201930965561
lack of evidence of sylvatic transmission of dengue viruses in the amazon rainforest near iquitos, peru.dengue viruses (denv) are currently responsible for more human morbidity and mortality than any other known arbovirus, and all four denv are known to exist in sylvatic cycles that might allow these viruses to persist if the urban (aedes aegypti) cycle could be controlled. to determine whether denv were being maintained in a sylvatic cycle in a forested area about 14 km southwest of iquitos, peru, a city in which all 4 serotypes of denv circulate, we placed 20 denv seronegative aotus monkeys in c ...201930964397
green synthesis of copper oxide nanoparticles and mosquito larvicidal activity against dengue, zika and chikungunya causing vector aedes the present study, high purity copper oxide nanoparticles (nps) were synthesised using tridax procumbens leaf extract. green syntheses of nano-mosquitocides rely on plant compounds as reducing and stabilising agents. copper oxide nps were characterised using x-ray diffraction (xrd) analysis, fourier transform infrared (ft-ir), field-emission scanning electron microscopy with energy dispersive spectroscopy, ultraviolet-visible spectrophotometry and fluorescence spectroscopy. xrd studies of the ...201830964011
impacts of hurricanes irma and maria on aedes aegypti populations, aquatic habitats, and mosquito infections with dengue, chikungunya, and zika viruses in puerto rico.puerto rico was severely impacted by hurricanes irma and maria in september 2017. the island has been endemic for dengue viruses (denv) and recently suffered epidemics of chikungunya (chikv 2014) and zika (zikv 2016) viruses. although severe storms tend to increase the number of vector and nuisance mosquitoes, we do not know how they influence aedes aegypti populations and arboviral transmission. we compared the abundance of female ae. aegypti in autocidal gravid ovitraps (ago traps), container ...201930963992
publisher correction: past and future spread of the arbovirus vectors aedes aegypti and aedes albopictus.this article was mistakenly not made open access when originally published; this has now been amended, and information about the creative commons attribution 4.0 international license has been added into the 'additional information' section.201930962571
differences in mosquito communities in six cities in oklahoma.vector-borne diseases in the united states have recently increased as a result of the changing nature of vectors, hosts, reservoirs, pathogens, and the ecological and environmental conditions. current information on vector habitats and how mosquito community composition varies across space and time is vital to successful vector-borne disease management. this study characterizes mosquito communities in urban areas of oklahoma, united states, an ecologically diverse region in the southern great pl ...201930950499
vector competence of australian aedes aegypti and aedes albopictus for an epidemic strain of zika virus.recent epidemics of zika virus (zikv) in the pacific and the americas have highlighted its potential as an emerging pathogen of global importance. both aedes (ae.) aegypti and ae. albopictus are known to transmit zikv but variable vector competence has been observed between mosquito populations from different geographical regions and different virus strains. since australia remains at risk of zikv introduction, we evaluated the vector competence of local ae. aegypti and ae. albopictus for a braz ...201930946747
linking water quality to aedes aegypti and zika in flood-prone neighborhoods.the ability of ecosystems to regulate water quality and flood events has been linked to health outcomes, including mosquito-borne illnesses. in the san juan bay estuary watershed of puerto rico, habitat alterations and land-use development have disrupted watershed hydrology, exacerbating wastewater discharges and subjecting some neighborhoods to frequent flooding events. in 2016, the mosquito-borne illness zika became a new cause for concern. we hypothesized that nutrient-enriched flood water co ...201930945160
interpreting mosquito feeding patterns in australia through an ecological lens: an analysis of blood meal studies.mosquito-borne pathogens contribute significantly to the global burden of disease, infecting millions of people each year. mosquito feeding is critical to the transmission dynamics of pathogens, and thus it is important to understanding and interpreting mosquito feeding patterns. in this paper we explore mosquito feeding patterns and their implications for disease ecology through a meta-analysis of published blood meal results collected across australia from more than 12,000 blood meals from 22 ...201930944025
v1016g point mutation: the key mutation in the voltage-gated sodium channel (vgsc) gene of pyrethroid-resistant aedes aegypti (diptera: culicidae) in indonesia.resistance to pyrethroid insecticides is widespread in indonesian aedes aegypti (linnaeus), the primary vector of dengue viruses. this study aims to investigate the mutations in the voltage-gated sodium channel (vgsc) conferring pyrethroid resistance against ae. aegypti populations from indonesia. molecular genotyping of mutations using polymerase chain reaction assay and direct dna sequencing were performed at positions 989 and 1,016 in iis6 region, and 1,534 in iiis6 region of the voltage-gate ...201930942885
influence of strategic points in the dispersion of aedes aegypti in infested evaluate whether sites with large amount of potential breeding sites for immature forms of aedes aegypti, called strategic points, influence in the active vector's dispersion into properties in their surroundings.201930942271
detrimental effects of induced antibodies on aedes aegypti reproduction.aedes aegypti (linnaeus) (diptera: culicidae) is the main vector of viruses causing dengue, chikungunya, zika, and yellow fever, worldwide. this report focuses on immuno-blocking four critical proteins in the female mosquito when fed on blood containing antibodies against ferritin, transferrin, one amino acid transporter (naat1), and acetylcholinesterase (ache). peptides from these proteins were selected, synthetized, conjugated to carrier proteins, and used as antigens to immunize new zealand r ...201930941675
correction to: complete nmr chemical shift assignments of odorant binding protein 22 from the yellow fever mosquito, aedes aegypti, bound to arachidonic acid.the article listed above was initially published with incorrect copyright information. upon publication of this correction, the copyright of the article is changed to "the author(s)". the original article has been corrected.201930941584
glycoprotein hormone receptor knockdown leads to reduced reproductive success in male aedes aegypti.glycoprotein hormone receptors mediate a diverse range of physiological functions in vertebrate and invertebrate organisms. the heterodimeric glycoprotein hormone gpa2/gpb5 and its receptor lgr1, constitute a recently discovered invertebrate neuroendocrine signaling system that remains to be functionally characterized. we previously reported that lgr1 is expressed in the testes of adult aedes aegypti mosquitoes, where its immunoreactivity is particularly regionalized. here, we show that lgr1 imm ...201930941056
enhanced mosquitocidal efficacy of colloidal dispersion of pyrethroid nanometric emulsion with benignity towards non-target species.the rising threat of vector-borne diseases and environmental pollution has instigated the investigation of nanotechnology-based applications. the current study deals with a nanotechnological application involving the usage of nanometric pesticides such as permethrin nanoemulsion. the mean droplet diameter and zeta potential of the prepared permethrin nanoemulsion were found to be 12.4 ± 1.13 nm and -20.4 ± 0.56 mv, respectively. the temporal stability of permethrin nanoemulsion was found to be 4 ...201930939406
endogenous non-retroviral elements in genomes of aedes mosquitoes and vector competence.recent extensive (re)emergences of arthropod-borne viruses (arboviruses) such as chikungunya (chikv), zika (zikv) and dengue (denv) viruses highlight the role of the epidemic vectors, aedes aegypti and aedes albopictus, in their spreading. differences of vector competence to arboviruses highlight different virus/vector interactions. while both are highly competent to transmit chikv (alphavirus,togaviridae), only ae. albopictus is considered as a secondary vector for denv (flavivirus, flavivirida ...201930938223
larvicidal, histopathological, antibacterial activity of indigenous fungus penicillium sp. against aedes aegypti l and culex quinquefasciatus (say) (diptera: culicidae) and its acetylcholinesterase inhibition and toxicity assessment of zebrafish (danio rerio).fungal metabolites are considered to be most efficient tools to overcome the issues related to insecticide resistance and environmental pollution. the present study focus on the evaluation of the mosquito larvicidal efficacy of metabolites of seven indigenous fungal isolates (penicillium sp. aspergillus niger, a. flavus, a. parasiticus, rhizopus sp. mucor sp. and aspergillus sp.) on the larvae of aedes aegypti and culex quinquefasciatus under the laboratory condition. the preliminary screening o ...201930936853
local transmission of zika virus in miami-dade county: the florida department of health rises to the early as 2015, florida and centers for disease control and prevention (cdc) public health officials recognized the potential danger of zika for us residents and visitors. the zika virus, a mosquito-borne flavivirus, is transmitted through the bite of the aedes aegypti mosquito. a physician in miami-dade notified the florida department of health (doh) of the first non-travel-related zika case in the united states. a 23-year old pregnant woman had presented on july 7, 2016, at 23 weeks of gesta ...201930933006
aedes aegypti mosquitoes detect acidic volatiles found in human odor using the ir8a pathway.mosquitoes use olfaction as a primary means of detecting their hosts. previously, the functional ablation of a family of aedes aegypti olfactory receptors, the odorant receptors (ors), was not sufficient to reduce host seeking in the presence of carbon dioxide (co2). this suggests the olfactory receptors that remain, such as the ionotropic receptors (irs), could play a significant role in host detection. to test this, we disrupted the ir8a co-receptor in ae. aegypti using crispr/cas9. we found t ...201930930038
evaluation of toxicity and environmental safety in use of spinosad to rationalize control strategies against aedes aegypti.spinosad is a naturally-occurring insecticide used for the management of ae. aegypti larvae. the assessment of ecotoxicological parameters of spinosad is required for verifying the environmentally-friendly behavior of the compound and for evaluating toxicity values on non-target species. thus, the aim of the study was to conduct toxicity tests using daphnia magna as model organism after exposure to different concentrations of spinosad. immobility effects were observed in both acute and chronic t ...201930927668
preliminary screening of mosquito spatial distribution in togo: with special focus on the aedes (diptera: culicidae) species.the togolese republic has a tropical and humid climate which constitutes an ideal environment for mosquitoes to breed and transmit diseases. the aedes mosquito is known to transmit yellow fever (yf), dengue, chikungunya, and zika viruses in west africa. togo has been suffering from yf virus transmission, despite vaccination efforts. unfortunately, there is scarcity in the data that reflect mosquito spatial distribution in togo, specifically possible yf vectors. in the current study, mosquito sur ...201930927005
voltage-gated sodium channel intron polymorphism and four mutations comprise six haplotypes in an aedes aegypti population in taiwan.knockdown resistance (kdr) to dichlorodiphenyltrichloroethane (ddt) and pyrethroids is known to link amino acid substitutions in the voltage-gated sodium channel (vgsc) in aedes aegypti. dengue fever primarily transmitted by ae. aegypti is an annual public health issue in taiwan. accordingly, pyrethroid insecticides have been heavily used for decades to control mosquito populations in the summer and autumn. in taiwan, an ae. aegypti population with two vgsc mutations, v1016g and d1763y, was desc ...201930925149
the electronic song "scary monsters and nice sprites" reduces host attack and mating success in the dengue vector aedes aegypti.sound and its reception are crucial for reproduction, survival, and population maintenance of many animals. in insects, low-frequency vibrations facilitate sexual interactions, whereas noise disrupts the perception of signals from conspecifics and hosts. despite evidence that mosquitoes respond to sound frequencies beyond fundamental ranges, including songs, and that males and females need to struggle to harmonize their flight tones, the behavioral impacts of music as control targets remain unex ...201930922800
use of mechanical and behavioural methods to eliminate female aedes aegypti and aedes albopictus for sterile insect technique and incompatible insect technique separation of mosquitoes at different stages is currently being attempted to ensure the successful release of male mosquitoes in novel vector control approaches. mechanical and behavioral techniques have been tried most frequently.201930922368
investigating the probability of establishment of zika virus and detection through mosquito surveillance under different temperature conditions.because of the increasing threat that zika virus (zikv) poses to more sub-tropical area due to increased global travel, there is a need for better understanding of the effect(s) of temperature on the establishment potential of zikv within these subtropical, temperate, and/or seasonal ae. aegypti populations. the first step to determining risk establishment of zikv in these regions is to assess zikv's ability to infect mosquitoes at less tropical temperatures, and thus be detected through common ...201930921386
global expansion and redistribution of aedes-borne virus transmission risk with climate change.forecasting the impacts of climate change on aedes-borne viruses-especially dengue, chikungunya, and zika-is a key component of public health preparedness. we apply an empirically parameterized model of viral transmission by the vectors aedes aegypti and ae. albopictus, as a function of temperature, to predict cumulative monthly global transmission risk in current climates, and compare them with projected risk in 2050 and 2080 based on general circulation models (gcms). our results show that if ...201930921321
modelling the potential distribution of arbovirus vector aedes aegypti under current and future climate scenarios in taiwan, china.aedes aegypti is one of the most important mosquito species and is a common disease-transmitting pest in tropical areas. various infectious arbovirus diseases can be transmitted by ae. aegypti. with ongoing global climate change, we are facing an increasing public health threat from the rapid spread of disease vectors into wider geographical areas. to better understand the current ecological niche range and possible future expansion of ae. aegypti, an ecological niche modelling approach was adop ...201930919547
wing geometry analysis of aedes aegypti (diptera, culicidae), a dengue virus vector, from multiple geographical locations of samut songkhram, thailand.dengue haemorrhagic fever (dhf) is a mosquito-borne disease and remains a major public health problem, especially in tropical and temperate countries. studying wing morphometric of aedes aegypti as a mosquito vector of dhf can help to better understand biological process of the mosquito adaptation to the environment. we aimed to study the geometric morphometric of ae. aegypti from multiple geographical areas.201830918904
vertical transmission of zika virus in culex quinquefasciatus say and aedes aegypti (l.) mosquitoes.several mosquito species have been described as vectors for the zika virus (zikv), such as those in the aedes, anopheles, mansonia and culex genera. our previous survey studies were found the zikv rna positive in both male, female and larvae of culex quinquefasciatus say and aedes aegypti (l.) mosquitoes collected from active zikv infected patients' homes in thailand. therefore, the aims of this study were to investigate whether zikv could be vertically transmitted in cx. quinquefasciatus, ae. a ...201930918310
evaluation of insecticide resistance in aedes aegypti populations connected by roads and rivers: the case of tocantins state in brazil.the longstanding application of insecticides for vector control without periodic monitoring of the population response to these chemicals can directly drive the selection of resistant populations of vector mosquitoes. tocantins is the newest state of the brazilian federation. despite a historically high number of dengue cases, studies and monitoring data concerning insecticide resistance in the state are lacking.201930916115
understanding the mechanism of chikungunya virus vector competence in three species of mosquitoes.chikungunya virus (chikv) is primarily transmitted by aedes spp. mosquitoes. the present study investigated vector competence for chikv in aedes aegypti and aedes albopictus mosquitoes found in madurai, south india. the role of receptor proteins on midguts contributing to permissiveness of chikv to aedes spp. mosquitoes was also undertaken. mosquitoes were orally infected with chikv drde-06. infection of midguts and dissemination to heads was confirmed by immunofluorescence assay at different ti ...201930913314
mosquitoes on a diet reduce those pesky bites.targeting the internal regulation of mosquito's human-seeking capacity provides a novel means for vector control. mosquitoes bite us to obtain blood in order to develop their eggs. professor vosshall and colleagues (cell 2019;176:687-701) have exploited the modulatory pathway to control this hunger for blood by designing drugs based on human diet-suppression pharmaceuticals.201930910491
high-throughput screening method for evaluating spatial repellency and vapour toxicity to mosquitoes.spatial repellents are an essential tool for personal protection against mosquitoes that bite and transmit disease pathogens to humans. current repellent screening methods, such as olfactometers and alternative choice tests, are complex systems that require a relatively large quantity of compound (mg). the present study validates a high-throughput spatial repellent screening method using a glass tube that has the ends covered with netting, in addition to treated filters and plastic end caps. the ...201930907445
mitochondrial affectation, dna damage and ache inhibition induced by salvia officinalis essential oil on aedes aegypti larvae.the aim of this research study was to understand the mechanism of action of salvia officinalis (lamiaceae) essential oil (eo) on aedes aegypti larvae. we evaluated the effect on dna damage, acetylcholinesterase (ache) inhibition and mitochondrial enzymatic alterations. the major components were analyzed in silico using osiris and molispiration free software. aedes aegypti dna was extracted from mosquito larvae between third (l3) and fourth (l4) instars to determine the dna fragmentation or degra ...201930905844
phytochemical composition and larvicidal activity of essential oils from herbal plants.the essential oils (eos) of plectranthus amboinicus showed the highest larvicidal activity among four herbal plants studied and β-caryophyllene might be the major component responsible for its differential toxicity to the larvae of culex quinquefasciatus and aedes aegypti. mosquitoes act as vectors for many life-threatening diseases, including malaria, dengue fever, and zika virus infection. management of mosquitoes mainly relies on synthetic insecticides, which usually result in the rapid devel ...201930904944
publisher correction: past and future spread of the arbovirus vectors aedes aegypti and aedes the version of this article originally published, the affiliation for author catherine linard was incorrectly stated as '6department of infectious disease epidemiology, london school of hygiene and tropical medicine, london, uk'. the correct affiliation is '9spatial epidemiology lab (spell), universite libre de bruxelles, brussels, belgium'. the affiliation for author hongjie yu was also incorrectly stated as '11department of statistics, harvard university, cambridge, ma, usa'. the correct af ...201930903094
aedes mosquitoes acquire and transmit zika virus by breeding in contaminated aquatic environments.zika virus (zikv) is a mosquito-borne flavivirus that predominantly circulates between humans and aedes mosquitoes. clinical studies have shown that zika viruria in patients persists for an extended period, and results in infectious virions being excreted. here, we demonstrate that aedes mosquitoes are permissive to zikv infection when breeding in urine or sewage containing low concentrations of zikv. mosquito larvae and pupae, including from field aedes aegypti can acquire zikv from contaminate ...201930902991
self-assembly of chlorin-e6 on γ-fe2o3 nanoparticles: application for larvicidal activity against aedes aegypti.aedes aegypti mosquitos are widespread vectors of several diseases and their control is of primary importance for biological and environmental reasons, and novel safe insecticides are highly desirable. an eco-friendly photosensitizing magnetic nanocarrier with larvicidal effects on aedes aegypti was proposed. the innovative core-shell hybrid nanomaterial was synthesized by combining peculiar magnetic nanoparticles (called surface active maghemite nanoparticles - samns, the core) and chlorin-e6 a ...201930897400
male accessory gland molecules inhibit harmonic convergence in the mosquito aedes aegypti.aedes aegypti mosquitoes transmit pathogens such as yellow fever, dengue, zika, and chikungunya viruses to millions of human hosts annually [1]. as such, understanding ae. aegypti courtship and mating biology could prove crucial to the success of disease control efforts that target reproduction. potentially to communicate reproductive fitness [2,3], mosquito males and females harmonize their flight tones prior to mating in a behavior known as harmonic convergence (hc) [4]. furthermore, after mat ...201930889386
environmental concentrations of antibiotics may diminish wolbachia infections in aedes aegypti (diptera: culicidae).wolbachia-infected aedes aegypti (l.) mosquitoes for control of dengue transmission are being released experimentally in tropical regions of australia, south-east asia, and south america. to become established, the wolbachia hertig (rickettsiales: rickettsiaceae) strains used must induce expression of cytoplasmic incompatibility (ci) in matings between infected males and uninfected females so that infected females have a reproductive advantage, which will drive the infection through field popula ...201930889242
climate change may enable aedes aegypti infestation in major european cities by 2100.climate change allows aedes aegypti to infest new areas. consequently, it enables the arboviruses the mosquito transmits -- e.g., dengue, chikungunya, zika and yellow fever - to emerge in previously uninfected areas. an example is the portuguese island of madeira during 2012-13.201930884421
update on the geographical distribution and prevalence of aedes aegypti and aedes albopictus (diptera: culicidae), two major arbovirus vectors in cameroon.arboviral diseases including dengue are increasingly spreading in the tropical/subtropical world including africa. updated knowledge on the distribution and abundance of the major vectors aedes aegypti and aedes albopictus constitutes crucial surveillance action to prepare african countries such as cameroon for potential arbovirus outbreaks. here, we present a nationwide survey in cameroon to assess the current geographical distribution and prevalence of both vectors including a genetic diversit ...201930883552
effects of prenatal exposure to temephos on behavior and social interaction.the neurodevelopment period is susceptible to alterations by genetic and environmental factors, such as the exposure to organophosphates (op). the op is neurotoxic and has been associated with neurological diseases pathophysiology. the op temephos is widely used against aedes aegypti in brazil's public health programs.201930880993
heterologous expression of aedes aegypti cation chloride cotransporter 2 (aeccc2) in xenopus laevis oocytes induces an enigmatic na⁺/li⁺ conductance.the yellow fever mosquito aedes aegypti possesses three genes encoding putative na⁺-coupled cation chloride cotransporters (cccs): aenkcc1, aeccc2, and aeccc3. to date, none of the aecccs have been functionally characterized. here we expressed aeccc2 heterologously in xenopus oocytes and measured the uptake of li⁺ (a tracer for na⁺) and rb⁺ (a tracer for k⁺). compared to control (h₂o-injected) oocytes, the aeccc2-expressing oocytes exhibited significantly greater uptake of li⁺, but not rb⁺. howe ...201930875796
biochemical and functional characterization of glycoside hydrolase family 16 genes in aedes aegypti larvae: identification of the major digestive β-1,3-glucanase.insect β-1,3-glucanases belong to glycoside hydrolase family 16 (ghf16) and are involved in digestion of detritus and plant hemicellulose. in this work, we investigated the role of ghf16 genes in aedes aegypti larvae, due to their detritivore diet. aedes aegypti genome has six genes belonging to ghf16 (aae gh16.1 - aae gh16.6), containing two to six exons. sequence analysis suggests that five of these ghf16 sequences (aae gh16.1, 2, 3, 5, and 6) contain the conserved catalytic residues of this f ...201930873040
development and validation of reverse transcription loop-mediated isothermal amplification (rt-lamp) for rapid detection of zikv in mosquito samples from brazil.the rapid spread of zika virus (zikv) represents a global public health problem, especially in areas that harbor several mosquito species responsible for virus transmission, such as brazil. in these areas, improvement in mosquito control needs to be a top priority, but mosquito viral surveillance occurs inefficiently in zikv-endemic countries. quantitative reverse transcription pcr (qrt-pcr) is the gold standard for molecular diagnostic of zikv in both human and mosquito samples. however, the te ...201930872672
the pharmacological and functional characterization of the serotonergic system in anopheles gambiae and aedes aegypti: influences on flight and blood-feeding behavior.aedes aegypti and anopheles gambiae harbor the causative agents of diseases such as dengue fever and malaria, afflicting human morbidity and mortality worldwide. given the worldwide emergence of resistance to insecticides, the current mainstay for vector control, identification of alternative modes of action for future insecticides is paramount. the serotonergic (5-ht) system has been documented to impact physiological mechanisms involved in disease transmission, suggesting its potential as a ne ...201930872615
protein expression in female salivary glands of pyrethroid-susceptible and resistant strains of aedes aegypti mosquitoes.a group of insecticides called pyrethroids has been used extensively worldwide and development of pyrethroid resistance within mosquito populations, especially in aedes aegypti, has rapidly spread through populations. in this study, sds-page, 2-de coupled with nanolc-ms, and bioinformatics were used to analyze the female salivary gland proteins of pyrethroid-susceptible (pmd) and pyrethroid-resistant (pmd-r and upk-r) strains of ae. aegypti mosquitoes for the first time.201930871604
evidence for the natural occurrence of wolbachia in aedes aegypti mosquitoes.wolbachia, a gram-negative bacterium, is widely known to be present in arthropods and nematodes. of late, great impetus is given to employ this intracellular bacterium, as an alternative to conventional biocontrol agents for the control of mosquitoes because of its inherent ability to induce sperm-egg incompatibility, feminisation etc. by employing molecular tools, we have shown the presence of wolbachia from aedes aegypti mosquito population collected from coimbatore, india by pcr amplifying th ...201930869785
corrigendum to "larvicidal potential of five selected dragonfly nymphs in sri lanka over aedes aegypti (linnaeus) larvae under laboratory settings".[this corrects the article doi: 10.1155/2018/8759459.].201930868069
first dengue virus seroprevalence study on madeira island after the 2012 outbreak indicates unreported dengue 2012, the first dengue virus outbreak was reported on the portuguese island of madeira with 1080 confirmed cases. dengue virus of serotype 1 (denv-1), probably imported from venezuela, caused this outbreak with autochthonous transmission by invasive aedes aegypti mosquitoes.201930867031
genome-wide divergence among invasive populations of aedes aegypti in the summer of 2013, aedes aegypti linnaeus was first detected in three cities in central california (clovis, madera and menlo park). it has now been detected in multiple locations in central and southern ca as far south as san diego and imperial counties. a number of published reports suggest that ca populations have been established from multiple independent introductions.201930866822
naturally infected aedes aegypti collected during a zika virus outbreak have viral titres consistent with transmission. 201930866777
costs of an aedes aegypti vector control program. 201930864616
larvicidal activity of selected essential oil in synergized combinations against aedes aegypti.this study was conducted to attain an alternate plant essential oil (eo) based mosquito larvicide against the dengue vector aedes aegypti. here three plants were selected based on their local availability, safety and insecticidal properties. eos were extracted from syzygium aromaticum (myrtaceae) flower buds, fruits of illicium verum (schisandraceae) and trachyspermum ammi (apiaceae) by hydro-distillation and tested against ae. aegypti larvae individually and in combinations to find synergistic ...201930861442
global outbreaks and origins of a chikungunya virus variant carrying mutations which may increase fitness for aedes aegypti: revelations from the 2016 mandera, kenya 2016, a chikungunya virus (chikv) outbreak was reported in mandera, kenya. this was the first major chikv outbreak in the country since the global reemergence of this virus in kenya in 2004. we collected samples and sequenced viral genomes from this outbreak. all kenyan genomes contained two mutations, e1:k211e and e2:v264a, recently reported to have an association with increased infectivity, dissemination, and transmission in the aedes aegypti vector. phylogeographic inference of temporal an ...201930860010
aedes aegypti agbr1 antibodies modulate early zika virus infection of mice.a recent epidemic of zika virus in the americas, affecting well over a million people, caused substantial mortality and morbidity, including guillain-barre syndrome, microcephaly and other fetal developmental defects1,2. preventive and therapeutic measures that specifically target the virus are not readily available. the transmission of zika virus is predominantly mosquito-borne, and aedes aegypti mosquitoes serve as a key vector for zika virus3. here, to identify salivary factors that modulate ...201930858571
modeling the impact of sterile males on an aedes aegypti population with optimal control.we use partial differential equations to describe the dynamics of an aedes aegypti mosquito population on an island, and the effects of a sterile male release. the model includes mosquito movement and an allee effect to capture extinction events. we apply optimal control theory to identify the release strategy that eliminates the mosquitoes most rapidly, conditional on a limited availability of sterile males. the optimal solution for a single location is to initially release a substantial number ...201930857942
laboratory evaluation of larvicidal and oviposition deterrent properties of edible plant oils for potential management of aedes aegypti (diptera: culicidae) in drinking water containers.the yellow fever mosquito, aedes aegypti (diptera: culicidae) transmits several devastating arboviruses, including dengue, chikungunya, and zika virus, making development of inexpensive and eco-friendly strategies for its control an urgent priority. we evaluated the lethality of 13 commonly used plant-derived edible oils against late-third instar ae. aegypti and then tested the three most lethal oils for stage-specific differences in lethality. we also examined the effects of the most lethal (he ...201930855084
udp-glycosyltransferase genes and their association and mutations associated with pyrethroid resistance in anopheles sinensis (diptera: culicidae).udp-glycosyltransferase (ugt) is an important biotransformation superfamily of enzymes. they catalyze the transfer of glycosyl residues from activated nucleotide sugars to acceptor hydrophobic molecules, and function in several physiological processes, including detoxification, olfaction, cuticle formation, pigmentation. the diversity, classification, scaffold location, characteristics, phylogenetics, and evolution of the superfamily of genes at whole genome level, and their association and muta ...201930845961
molecular epidemiology and genetic diversity of zika virus from field-caught mosquitoes in various regions of thailand.zika virus (zikv) infection is an emerging and re-emerging arbovirus disease that is transmitted to humans through the bite of infected mosquitoes. zikv infections were first described in thailand in 1954 from the sera of indigenous residents and several travelers returning from thailand in 2014. however, reported cases in thailand have been increasing since 2015 and 2016, and epidemiological information about the vectors of zikv is unclear. we investigated the molecular epidemiology and genetic ...201930845707
updated methods for the production of toxorhynchites rutilus septentrionalis (diptera, culicidae) for use as biocontrol agent against container breeding pest mosquitoes in harris county, texas.the complex biologies of pestiferous mosquito species complicate the development of a single, across the board effective control measure. the use of traditional biological control through predators, parasitoids, and microbes, though part of a multimodal, integrated pest management approach, is scant in current standard mosquito control operations. at this time, traditional, whole organism mosquito biocontrol agents are not commercially available, and if their integration into a release program i ...201930843583
urban and semi-urban mosquitoes of mexico city: a risk for endemic mosquito-borne disease transmission.since past century, vector-borne diseases have been a major public health concern in several states of mexico. however, mexico city continues to be free of endemic mosquito-borne viral diseases. the city is the most important politic and economic state of mexico and one of the most important city of latin america. its subtropical highland climate and high elevation (2240 masl) had historically made the occurrence of aedes species unlikely. however, the presence of other potential disease vectors ...201930840661
effects of multiple transmission pathways on zika dynamics.although the zika virus is transmitted to humans primarily through the bite of infected female aedes aegypti mosquitoes, it can also be sexually and vertically transmitted within both populations. in this study, we develop a new mathematical model of the zika virus which incorporates sexual transmission in humans and mosquitos, vertical transmission in mosquitos, and mosquito to human transmission through bites. analysis of this deterministic model shows that the secondary transmission routes of ...201830839920
activity of additives and their effect in formulations of metarhizium anisopliae s.l. ip 46 against aedes aegypti adults and on post mortem conidiogenesis.oil formulations of entomopathogenic fungi have interest for biological mosquito control.201930836061
epidemiological and clinical characteristics of dengue virus outbreaks in two regions of china, 2014 - 2015.dengue virus (denv), a single-stranded rna virus and flaviviridae family member, is transmitted by aedes aegypti and aedes albopictus mosquitoes. denv causes dengue fever, which may progress to severe dengue. hospital-based surveillance was performed in two chinese regions, guangzhou and xishuangbanna, during the dengue epidemics in 2014 and 2015, respectively. acute-phase serum was obtained from 133 patients with suspected dengue infections during the peak season for dengue cases. viremia level ...201930835769
transcriptome sequencing and analysis of changes associated with insecticide resistance in the dengue mosquito (aedes aegypti) in vietnam.the mosquito aedes aegypti is a transmission vector for dangerous epidemic diseases in humans. insecticides have been used as the most general vector control method in the world. however, ae. aegypti have developed many resistant mechanisms such as reduced neuronal sensitivity to insecticides (target-site resistance), enhanced insecticide metabolism (metabolic resistance), altered transport, sequestration, and other mechanisms. it has become a major problem for vector control programs. transcrip ...201930834881
past and future spread of the arbovirus vectors aedes aegypti and aedes albopictus.the global population at risk from mosquito-borne diseases-including dengue, yellow fever, chikungunya and zika-is expanding in concert with changes in the distribution of two key vectors: aedes aegypti and aedes albopictus. the distribution of these species is largely driven by both human movement and the presence of suitable climate. using statistical mapping techniques, we show that human movement patterns explain the spread of both species in europe and the united states following their intr ...201930833735
discovery and characterization of 2-nitro-5-(4-(phenylsulfonyl)piperazin-1-yl)- n-(pyridin-4-ylmethyl)anilines as novel inhibitors of the aedes aegypti kir1 ( aekir1) channel.mosquito-borne arboviral diseases such as zika, dengue fever, and chikungunya are transmitted to humans by infected adult female aedes aegypti mosquitoes and affect a large portion of the world's population. the kir1 channel in ae. aegypti ( aekir1) is an important ion channel in the functioning of mosquito malpighian (renal) tubules and one that can be manipulated in order to disrupt excretory functions in mosquitoes. we have previously reported the discovery of various scaffolds that are activ ...201930832472
characterization, diversity, and structure-activity relationship study of lipoamino acids from pantoea sp. and synthetic analogues.a biological evaluation of a library of extracts from entomopathogen strains showed that pantoea sp. extract has significant antimicrobial and insecticidal activities. three hydroxyacyl-phenylalanine derivatives were isolated from this strain. their structures were elucidated by a comprehensive analysis of their nmr and ms spectroscopic data. the antimicrobial and insecticidal potencies of these compounds were evaluated, and compound 3 showed 67% mortality against aedes aegypti larvae at a conce ...201930832353
determination by chromatography and cytotoxotoxic and oxidative effects of pyriproxyfen and pyridalyl.pyriproxyfen (ppf) is a larvicide, used to combat the proliferation of aedes aegypti larvae. the objective of this study was to analyze the compounds of pyriproxyfen and pyridalyl (pyl) in a commercial larvicide to analyze the cytotoxic and oxidative effects of ppf and pyl. the toxic potential of ppf and pyl were assessed based on lethal concentration (lc50) in artemia salina, cytotoxicity based on the mitotic index and the chromosomal alterations in allium cepa and the oxidative damage in sacch ...201930831490
effects of methylmercury on mosquito oviposition behavior: maladaptive response to non-toxic exposure.animals can modulate their own exposure to environmental contaminants through behavioral plasticity such as diet and habitat choice. however, it remains unclear if behavior also has cascading effects on contaminant exposure across multiple generations. in insects, oviposition site selection is an important behavior females can use to modify offspring contaminant exposure risk. in this study, we use the yellow fever mosquito, aedes aegypti, to test how methylmercury (mehg) affects oviposition sit ...201930831364
protocols for testing the toxicity of novel insecticidal chemistries to classes of insecticides with novel modes of action are needed to control insecticide resistant populations of mosquitoes that transmit diseases such as zika, dengue and malaria. assays for rapid, high-throughput analyses of unformulated novel chemistries against mosquito larvae and adults are presented. we describe protocols for single point-dose and dose response assays to evaluate the toxicity of small molecule chemistries to the aedes aegypti vector of zika, dengue and yellow fever, the m ...201930829331
a comprehensive assessment of inbreeding and laboratory adaptation in aedes aegypti mosquitoes.modified aedes aegypti mosquitoes reared in laboratories are being released around the world to control wild mosquito populations and the diseases they transmit. several efforts have failed due to poor competitiveness of the released mosquitoes. we hypothesized that colonized mosquito populations could suffer from inbreeding depression and adapt to laboratory conditions, reducing their performance in the field. we established replicate populations of ae. aegypti mosquitoes collected from queensl ...201930828375
the first outbreak of dengue fever in greater darfur, western sudan.dengue virus (denv) is an arthropod-borne virus (arbovirus) transmitted by the aedes mosquitoes, mainly aedes aegypti. dengue fever is a rapidly growing disease with expanding geographical distribution worldwide. we investigated a high number of non-malaria febrile cases reported to health clinics in refugee camps in the five states of darfur between august 2015 and march 2016. the clinical presentation of cases and case definition criteria suggested involvement of one or more arboviral hemorrha ...201930823624
trap location and premises condition influences on aedes aegypti (diptera: culicidae) catches using biogents sentinel traps during a 'rear and release' program: implications for designing surveillance the incidence of arboviral diseases such as dengue, zika, chikungunya, and yellow fever increases globally, controlling their primary vector, aedes aegypti (l.) (diptera: culicidae), is of greater importance than ever before. mosquito control programs rely heavily on effective adult surveillance to ensure methodological efficacy. the biogents sentinel (bgs) trap is the gold standard for surveilling adult aedes mosquitoes and is commonly deployed worldwide, including during modern 'rear and re ...201930817823
efficacy of novel indoor residual spraying methods targeting pyrethroid-resistant aedes aegypti within experimental houses.challenges in maintaining high effectiveness of classic vector control in urban areas has renewed the interest in indoor residual spraying (irs) as a promising approach for aedes-borne disease prevention. while irs has many benefits, application time and intrusive indoor applications make its scalability in urban areas difficult. modifying irs to account for ae. aegypti resting behavior, named targeted irs (tirs, spraying walls below 1.5 m and under furniture) can reduce application time; howeve ...201930817759
socio-ecological factors associated with dengue risk and aedes aegypti presence in the galápagos islands, ecuador.dengue fever is an emerging infectious disease in the galápagos islands of ecuador, with the first cases reported in 2002 and subsequent periodic outbreaks. we report results of a 2014 pilot study conducted in puerto ayora (pa) on santa cruz island, and puerto baquerizo moreno (pb) on san cristobal island. to assess the socio-ecological risk factors associated with dengue and mosquito vector presence at the household level, we conducted 100 household surveys (50 on each island) in neighborhoods ...201930813558
assessment of the effectiveness of bg-sentinel traps baited with co2 and bg-lure for the surveillance of vector mosquitoes in miami-dade county, florida.vector-borne diseases are an increasing issue to public health, endangering billions of people worldwide. controlling vector mosquitoes is widely accepted as the most effective way to prevent vector-borne disease outbreaks. mosquito surveillance is critical for the development of control strategies under the integrated vector management framework. we hypothesize that the effectiveness and reliability of using bg-sentinel traps for the surveillance strongly depend on the bait used to attract mosq ...201930794670
widespread insecticide resistance in aedes aegypti l. from new mexico, u.s.a.aedes aegypti mosquitoes are vectors of a variety of emerging viral pathogens, including yellow fever, dengue, chikungunya, and zika virus. this species has established endemic populations in all cities across southern new mexico sampled to date. presently, control of aedes-borne viruses relies on deployment of insecticides to suppress mosquito populations, but the evolution of insecticide resistance threatens the success of vector control programs. while insecticide resistance is quite common i ...201930794644
high frequencies of f1534c and v1016i kdr mutations and association with pyrethroid resistance in aedes aegypti from somgandé (ouagadougou), burkina faso.resistance to pyrethroid insecticides involving kdr mutations is widespread in aedes aegypti (l.) (diptera: culicidae) and potentially could impact control efforts in endemic countries. dengue cases had been sporadic in burkina faso for over a decade prior to the 2016-2017 outbreak that resulted in 15,074 suspected cases and 36 deaths, mainly in ouagadougou. these outbreaks highlighted the lack of information on numerous aspects of the biology, behaviour and insecticide status of local dengue ve ...201930787670
experimental study on the action of larvicides in aedes aegypti populations collected in the brazilian municipality of itabuna, bahia, under simulated field evaluate, under simulated field conditions, the efficacy of pyriproxyfen (juvenile hormone), novaluron (chitin inhibitor) and spinosad (biolarvicide) in controlling aedes aegypti.201930785572
entomological and virological surveillance for dengue virus in churches in merida, mexico.this study was designed to assess whether churches in endemic dengue districts in merida, mexico provide suitable breeding habitats for mosquitoes and are potential sites for dengue virus (denv) transmission. churches were inspected for immature and adult mosquitoes once every week from november 2015 to october 2016. a total of 10,997 immatures of five species were collected. the most abundant species were aedes aegypti (6,051) and culex quinquefasciatus (3,018). the most common source of immatu ...201930785563
origin and expansion of the mosquito aedes aegypti in madeira island (portugal).historically known as the yellow fever mosquito, aedes aegypti invaded madeira island in 2005 and was the vector of the island's first dengue outbreak in 2012. we have studied genetic variation at 16 microsatellites and two mitochondrial dna genes in temporal samples of madeira island, in order to assess the origin of the invasion and the population structure of this mosquito vector. our results indicated at least two independent colonization events occurred on the island, both having a south am ...201930783149
optimization of irradiation dose to aedes aegypti and ae. albopictus in a sterile insect technique program.the sterile insect technique (sit) may offer a means to control the transmission of mosquito borne diseases. sit involves the release of male insects that have been sterilized by exposure to ionizing radiation. we determined the effects of different doses of radiation on the survival and reproductive capacity of local strains of aedes aegypti and ae. albopictus in southern mexico. the survival of irradiated pupae was invariably greater than 90% and did not differ significantly in either sex for ...201930779779
larvicidal and ovicidal activities of artocarpus blancoi extracts against aedes aegypti.dengue control may be done by targeting its vector. in this study, we used artocarpus blancoi (elm.) merr. (moraceae) leaves, an endemic hematophagous insect repellent as a larvicide and ovicide.201930776315
natural vertical infection by dengue virus serotype 4, zika virus and mayaro virus in aedes (stegomyia) aegypti and aedes (stegomyia) albopictus.vertical transmission to progeny ensures the maintenance of arboviruses in their natural vectors. this mechanism is largely reported for dengue virus (denv) and yellow fever virus (yfv). few studies have addressed this mechanism for zika virus (zikv), mayaro virus (mayv) and other arboviruses. the present study investigated the natural infection rate by arboviruses in 4490 aedes (stegomyia) aegypti and 296 aedes (stegomyia) albopictus (diptera: culicidae) reared from eggs collected with ovitraps ...201930776139
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