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downregulation of female doublesex expression by oral-mediated rna interference reduces number and fitness of anopheles gambiae adult females.mosquito-borne diseases affect millions worldwide, with malaria alone killing over 400 thousand people per year and affecting hundreds of millions. to date, the best strategy to prevent the disease remains insecticide-based mosquito control. however, insecticide resistance as well as economic and social factors reduce the effectiveness of the current methodologies. alternative control technologies are in development, including genetic control such as the sterile insect technique (sit). the sit i ...201930992032
gone in 60 seconds: sub-lethal effects of metofluthrin vapors on behavior and fitness of resistant and field strains of aedes aegypti (diptera: culicidae).spatial repellents can reduce fecundity and interrupt oviposition behavior in aedes aegypti. yet, it is unclear if short exposure times, resistant phenotypes, and other aspects of spatial repellents can impact these effects on mosquito reproduction. to address these issues, pyrethroid susceptible, pyrethroid resistant, and field strains of ae. aegypti were used to evaluate the extent to which fecundity and oviposition behavior are affected following metofluthrin exposure. mosquitoes were exposed ...201930989189
prevalence of kdr mutations and insecticide susceptibility among natural population of aedes aegypti in west bengal.aedes albopictus and aedes aegypti are the major vectors of arboviral diseases. as effective vaccines are not available for most of the arboviral diseases, vector control by using insecticides play the key role to reduce the disease transmission. the emergence and spread of resistance to different classes of insecticides by the vectors is a major obstacle to control the disease transmission. information about vector susceptibility to different insecticides and their mechanisms are very important ...201930986273
aedes aegypti hpx8c modulates immune responses against viral infection.mosquitoes act as vectors of numerous pathogens that cause human diseases. dengue virus (denv) transmitted by mosquito, aedes aegypti, is responsible for dengue fever epidemics worldwide with a serious impact on human health. currently, disease control mainly relies on vector targeted intervention strategies. therefore, it is imperative to understand the molecular mechanisms underlying the innate immune response of mosquitoes against pathogens. in the present study, the expression profiles of im ...201930986216
target and non-target botanical pesticides effect of trichodesma indicum (linn) r. br. and their chemical derivatives against the dengue vector, aedes aegypti l.the effects of crude ethanol derived leaf extract trichodesma indicum (linn) (ex-ti) and their chief derivatives were accessed on the survival and development of the dengue mosquito ae. aegypti also their non-toxic activity against mosquito predator. t. indicum is recognized to be the vital weed plant and a promising herb in the traditional ayurvedic medicine. in this study, the gc-ms chromatogram of ex-ti showed higher peak area percentage for cis-10-heptadecenoic acid (21.83%) followed by cycl ...201930977009
hsp superfamily of genes in the malaria vector anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance.heat shock proteins (hsps) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress. there is still no report regarding the diversity and phylogenetics research of hsp superfamily of genes at whole genome level in insects, and the hsp gene association with pyrethroid resistance is also not well known. the present study investigated the diversity, classification, scaffold location, characteristics, and phylogenetics of the superfamily ...201930975215
neem oil increases the persistence of the entomopathogenic fungus metarhizium anisopliae for the control of aedes aegypti (diptera: culicidae) larvae.the entomopathogenic fungus metarhizium anisopliae is a candidate for the integrated management of the disease vector mosquito aedes aegypti. metarhizium anisopliae is pathogenic and virulent against ae. aegypti larvae; however, its half-life is short without employing adjuvants. here, we investigated the use of neem oil to increase virulence and persistence of the fungus under laboratory and simulated field conditions.201930975207
a method for assessing mitochondrial physiology using mechanically permeabilized flight muscle of aedes aegypti mosquitoes.aedes aegypti is the most important and widespread vector of arboviruses, including dengue and zika. insect dispersal through the flight activity is a key parameter that determines vector competence, and is energetically driven by oxidative phosphorylation in flight muscle mitochondria. analysis of mitochondrial function is central for a better understanding of cellular metabolism, and is mostly studied using isolated organelles. however, this approach has several challenges and methods for asse ...201930974092
aedes aegypti nest1 protein enhances zika virus pathogenesis by activating neutrophils.saliva from the mosquito vector of flaviviruses is capable of changing the local immune environment, leading to an increase in flavivirus-susceptible cells at the infected bite site. in addition, an antibody response to specific salivary gland (sg) components changes the pathogenesis of flaviviruses in human populations. to investigate whether antigenic sg proteins are capable of enhancing infection with zika virus (zikv), a reemerging flavivirus primarily transmitted by the aedes aegypti mosqui ...201930971475
expanding the canon: non-classical mosquito genes at the interface of arboviral infection.mosquito transmitted viruses cause significant morbidity and mortality in human populations. despite the use of insecticides and other measures of vector control, arboviral diseases are on the rise. one potential solution for limiting disease transmission to humans is to render mosquitoes refractory to viral infection through genetic modification. substantial research effort in drosophila, aedes and anopheles has helped to define the major innate immune pathways, including toll, imd, jak/stat an ...201930970277
abiotic factors and population dynamic of aedes aegypti and aedes albopictus in an endemic area of dengue in brazil.aedes aegypti and aedes albopictus are exotic species in the americas with high epidemiological relevance as they are vectors of many pathogens. this study aimed at understanding the population dynamics of a. aegypti and a. albopictus and the influence of abiotic factors in an endemic area of dengue. the study was conducted in the urban area of campo grande, mato grosso do sul, brazil, over one year. in seven regions of the city, 50 ovitraps were installed in each neighborhood. the development o ...201930970109
evaluation of the larvicidal potential of the essential oil pogostemon cablin (blanco) benth in the control of aedes aegypti.the objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of pogostemon cablin leaves. the chemical characterization was performed by gas chromatography coupled to mass spectrometer (gc-ms). the larvicidal activity was performed according to the protocol of the world health organization. the antioxidant activity was ...201930965561
lack of evidence of sylvatic transmission of dengue viruses in the amazon rainforest near iquitos, peru.dengue viruses (denv) are currently responsible for more human morbidity and mortality than any other known arbovirus, and all four denv are known to exist in sylvatic cycles that might allow these viruses to persist if the urban (aedes aegypti) cycle could be controlled. to determine whether denv were being maintained in a sylvatic cycle in a forested area about 14 km southwest of iquitos, peru, a city in which all 4 serotypes of denv circulate, we placed 20 denv seronegative aotus monkeys in c ...201930964397
the efficiency of a new automated mosquito larval counter and its impact on larval survival.to achieve consistent and standardized rearing for mosquito immature stages, it is crucial to control the initial number of larvae present in each larval tray. in addition, maintaining an optimal and synchronized development rate of larvae is essential to maximize the pupal production and optimize male sorting in a mass-rearing setting. manual counting is labor intensive, time consuming and error prone. therefore, this study aimed to investigate the use of a customized automated counter for the ...201931092868
infection of aedes albopictus mosquito c6/36 cells with the wmelpop strain of wolbachia modulates dengue virus-induced host cellular transcripts and induces critical sequence alterations in the dengue viral genome.dengue virus (denv) causes frequent epidemics infecting ∼390 million people annually in over 100 countries. there are no approved vaccines or antiviral drugs for treatment of infected patients. however, there is a novel approach to control denv transmission by the mosquito vectors, aedes aegypti and aedes albopictus, using the wolbachia symbiont. the wmelpop strain of wolbachia suppresses denv transmission and shortens the mosquito life span. however, the underlying mechanism is poorly understoo ...201931092581
microencapsulation of citrus grandis peel oil using interfacial precipitation chemistry technique for repellent application.essential oil of citrus family plant is known to have repellent effect against mosquito. unfortunately, due to its high volatility effect, its repellency effect was compromised. the incorporation of essential oil in a microencapsulation formulation has been shown to help improve the stability and potency of the repellent. in this study, citrus grandis peel oil (cgpo) was encapsulated by using the interfacial precipitation chemistry technique. the microencapsulated cgpo was then formulated into l ...201931089355
larvicidal, adulticidal, and oviposition-deterrent activity of piper betle l. essential oil to aedes aegypti.aedes aegypti is a primary vector of many arthropod-borne diseases. one of the diseases, dengue fever, is an endemic disease in indonesia causing high mortalities for decades. there are no preventive and specific treatments for dengue so far. therefore, prevention of this disease largely depends on the mosquito control. since resistance to chemical insecticides occurred worldwide, the study on alternate and new mosquito insecticides are mandatory. this study aimed to demonstrate the effect of es ...201931089304
efficacy of the in2care® auto-dissemination device for reducing dengue transmission: study protocol for a parallel, two-armed cluster randomised trial in the philippines.mosquito-borne viruses are imposing an ever increasing health burden worldwide. in addition to the recent zika and chikungunya virus epidemics, dengue viruses have become the fastest growing problem with a 40-fold increase in the number of reported cases over the past five decades. current mosquito control techniques involving larval source reduction, larviciding, and space spray of adulticides are costly, laborious, and of debatable efficacy. there remains an urgent need for the development of ...201931088515
phylogeography and invasion history of aedes aegypti, the dengue and zika mosquito vector in cape verde islands (west africa).aedes-borne arboviruses have spread globally with outbreaks of vast impact on human populations and health systems. the west african archipelago of cape verde had its first outbreak of dengue in 2009, at the time the largest recorded in africa, and was one of the few african countries affected by the zika virus epidemic. aedes aegypti was the mosquito vector involved in both outbreaks. we performed a phylogeographic and population genetics study of a. aegypti in cape verde in order to infer the ...201931548858
panophthalmitis associated with scleral necrosis in dengue hemorrhagic fever.dengue is a mosquito-borne flavivirus disease affecting humans. the aedes aegypti mosquito spreads it. ophthalmic manifestations of dengue range from subconjunctival hemorrhage to optic neuropathy. panophthalmitis in dengue fever is a rare finding. we report a case of a 22-year-old male having dengue fever, who presented with pain, redness, swelling and loss of vision in his right eye. he was diagnosed as panophthalmitis with subretinal hemorrhage and required right eye evisceration.201931546563
linking nutrient stoichiometry to zika virus transmission in a mosquito.food quality and quantity serve as the basis for cycling of key chemical elements in trophic interactions; yet the role of nutrient stoichiometry in shaping host-pathogen interactions is under appreciated. most of the emergent mosquito-borne viruses affecting human health are transmitted by mosquitoes that inhabit container systems during their immature stages, where allochthonous input of detritus serves as the basal nutrients. quantity and type of detritus (animal and plant) were manipulated i ...201931227906
lichen parmelia sulcata mediated synthesis of gold nanoparticles: an eco-friendly tool against anopheles stephensi and aedes aegypti.the gold nanoparticles (aunps) were synthesized using the lichen parmelia sulcata extract (pse) and characterized. the peaks of ultraviolet spectrophotometer and fourier transmission infrared confirmed the formation of nanoparticles and the bioactive compounds of the lichen being responsible for reducing and capping of the particles. the face-centered cubic particles were determined by xrd peaks at 111, 200, 220, and 311. the elemental composition and spherical shape of aunps were confirmed by e ...201931218582
how do trait-mediated non-lethal effects of predation affect population-level performance of mosquitoes?non-lethal, trait-mediated effects of predation impact prey behavior and life-history traits. studying how these effects in turn influence prey demography is crucial to understand prey life-history evolution. mosquitoes are important vectors that claim several million lives every year worldwide by transmitting a range of pathogens. several ecological factors affect life-history traits of both larval and adult mosquitoes, creating effects that cascade to population-level consequences. few studies ...201931218216
community composition and year-round abundance of vector species of mosquitoes make miami-dade county, florida a receptive gateway for arbovirus entry to the united states.vector-borne diseases are a heavy burden to human-kind. global warming and urbanization have a significant impact on vector-borne disease transmission, resulting in more severe outbreaks, and outbreaks in formerly non-endemic areas. miami-dade county, florida was the most affected area in the continental united states during the 2016 zika virus outbreak. miami is an important gateway and has suitable conditions for mosquitoes year-round. therefore, it was critical to establish and validate a sur ...201931217547
development of cs-tpp-dsrna nanoparticles to enhance rnai efficiency in the yellow fever mosquito, aedes aegypti.mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. vector control is one of the most important approaches used in reducing the incidence of these diseases. however, increasing mosquito resistance to chemical insecticides presents challenges to this approach. therefore, new strategies are necessary to develop the next generation vector control methods. because of the target specificity of dsrna, rnai-based control measures are ...201931217512
mosquitocidal chips containing the insect growth regulator pyriproxyfen for control of aedes aegypti (diptera: culicidae).aedes aegypti were exposed to water treated with mosquitocidal chips containing the insecticide pyriproxyfen in a polymer formulation. chips were tested under different conditions; different water volumes, in containers made of different material, and in water with different levels of organic matter. treated chips caused 100% mortality of ae. aegypti during their pupal stage independent of size or type of container, and the mount of organic matter contained in the water to which the mosquito lar ...201931216627
oviposition preferences of the mosquito aedes aegypti linnaeus, 1762 (culicidae): an urban environment bioassay.the establishment of an invasive species depends on reproductive success and dispersion capability in the new environment. one of the striking examples of invasion in urban environments is the mosquito aedes aegypti linnaeus, 1762 (culicidae). the success of this species is primarily attributed to its ability to colonize urban environments, and some of the important adaptive strategies associated with this ability is the preference for humans as a blood source and intense occupation of residenti ...201931213206
inferring who-infected-whom-where in the 2016 zika outbreak in singapore-a spatio-temporal model.singapore experienced its first known zika outbreak in 2016. given the lack of herd immunity, the suitability of the climate for pathogen transmission, and the year-round presence of the vector- aedes aegypti-zika had the potential to become endemic, like dengue. guillain-barré syndrome and microcephaly are severe complications associated elsewhere with zika and the risk of these complications makes understanding its spread imperative. we investigated the spatio-temporal spread of locally transm ...201931213175
asymptomatic dengue virus infections, cambodia, 2012-2013.we investigated dengue virus (denv) and asymptomatic denv infections in rural villages of kampong cham province, cambodia, during 2012 and 2013. we conducted perifocal investigations in and around households for 149 denv index cases identified through hospital and village surveillance. we tested participants 0.5-30 years of age by using nonstructural 1 rapid tests and confirmed denv infections using quantitative reverse transcription pcr or nonstructural 1-capture elisa. we used multivariable po ...201931211672
live calcium imaging of aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies.the mosquito aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. in particular, compared to ...201931208328
geometric morphometric analysis of the effect of temperature on wing size and shape in aedes albopictus.wing geometry helps to identify mosquito species, even cryptic ones. on the other hand, temperature has a well-known effect on insect metric properties. can such effects blur the taxonomic signal embedded in the wing? two strains of aedes albopictus (laboratory and field strain) were examined under three different rearing temperatures (26, 30 and 33 °c) using landmark- and outline-based morphometric approaches. the wings of each experimental line were compared with aedes aegypti. both approaches ...201931125148
new records of aedes aegypti and aedes albopictus in eight texas counties, u.s.a. 201931124239
spatial repellency and other effects of transfluthrin and linalool on aedes aegypti and aedes albopictus.in the present study, the effects of two spatial repellents (sr) were determined for aedes aegypti and ae albopictus, the main vectors of dengue, chikungunya, and zika fever. the modular high-throughput screening system (hitss) was used to evaluate the response of both species to transfluthrin and linalool sr at different concentrations. the highest spatial repellency results for ae. aegypti were obtained by transfluthrin to 0.001% with 37.50 ± 4.33%, and for linalool to 10% with 77.50 ± 3.90%. ...201931124222
diet drugs trick mosquitoes into feeling full.after a bloodmeal, aedesaegypti mosquitoes lose interest in bloodfeeding. duvall et al. (cell 2019;176:687-701) determined that the neuropeptide y (npy)-like receptor 7 (npylr7) controls mosquito satiety and also identified six npylr7 drug targets that suppress biting. this work highlights an innovative approach in vector control linking insect behavior to drug discovery.201931122765
excito-repellent activity of β-caryophyllene oxide against aedes aegypti and anopheles minimus.contact irritant and non-contact repellent activities of β-caryophyllene oxide were evaluated against laboratory strains of female aedes aegypti (usda strain), a major arbovirus vector and anopheles minimus (ku strain), a major malaria parasite vector, compared with the synthetic repellent deet, using an excito-repellency test system. β-caryophyllene oxide and deet were tested at concentrations of 0.1, 0.25, 0.5 and 1.0% (v/v). anopheles minimus was found to be more sensitive to β-caryophyllene ...201931121148
identification of molecular determinants of resistance to pyrethroid insecticides in aedes aegypti (diptera: culicidae) populations in california, usa.the first breeding populations of aedes aegypti (linnaeus) were identified in california in 2013, and have since been detected in 13 counties. recent studies suggest two introductions likely occurred, with genetically distinct populations in the central and southern regions of the state. given the threat of dengue, chikungunya, and zika virus transmission, it is imperative to understand if these populations harbor genes that could confer resistance to pyrethrin-based insecticides, known as pyret ...201931121042
uncertainty analysis of species distribution models.the maximum entropy model, a commonly used species distribution model (sdm) normally combines observations of the species occurrence with environmental information to predict the geographic distributions of animal or plant species. however, it only produces point estimates for the probability of species existence. to understand the uncertainty of the point estimates, we analytically derived the variance of the outputs of the maximum entropy model from the variance of the input. we applied the an ...201931120909
insecticide resistance levels and mechanisms in aedes aegypti populations in and around ouagadougou, burkina faso.recent outbreaks of dengue and other aedes aegypti-borne arboviruses highlight the importance of a rapid response for effective vector control. data on insecticide resistance and underlying mechanisms are essential for outbreak preparedness, but are sparse in much of africa. we investigated the levels and heterogeneity of insecticide resistance and mechanisms of ae. aegypti from contrasting settings within and around ouagadougou, burkina faso.201931120874
topological qsar modelling of carboxamides repellent activity to aedes aegypti.aedes aegypti vector control is of paramount importance owing to the damages induced by the various severe diseases that these insects may transmit, and the increasing risk of important outbreaks of these pathologies. search for new chemicals efficient against aedes aegypti, and devoid of side-effects, which have been associated to the currently most used active substance i. e. n,n-diethyl-m-toluamide (deet), is therefore an important issue. in this context, we developed various quantitative str ...201931120598
entomological efficacy of aerial ultra-low volume insecticide applications against aedes aegypti (diptera: culicidae) in mexico.a cluster-randomized controlled trial quantified the entomological efficacy of aerial ultra-low volume (aulv) applications of the insecticide chlorpyrifos against aedes aegypti in puerto vallarta, méxico, during november-october 2017. the trial involved 16 large (1 × 1 km) clusters distributed between treatment-control arms. primary endpoint was the abundance of ae. aegypti indoors (total adults, females, and blood-fed females) collected using prokopack aspirators. after four consecutive weekly ...201931120502
wolbachia strain walba blocks zika virus transmission in aedes aegypti.transinfections of the maternally transmitted endosymbiont wolbachia pipientis can reduce rna virus replication and prevent transmission by aedes aegypti, and also have the capacity to invade wild-type populations, potentially reaching and maintaining high infection frequencies. levels of virus transmission blocking are positively correlated with wolbachia intracellular density. despite reaching high densities in ae. aegypti, transinfections of walba, a strain native to aedes albopictus, showed ...201931120156
larvicidal activity of hypoestes forskaolii (vahl) r. br root extracts against anopheles gambiae giless.s, aedes aegypti l, and culex quinquefasciatussay.aim: this study aimed to evaluate larvicidal activity of hypoestes forskaolii r. br root extract against 3rd instar anopheles gambiae, aedes aegypti, and culex quinquefasciatus. methods: a protocol developed by the world health organization was adopted, with minor modification using chloroform and methanol extracts with concentrations ranging from 25-750 µg/ml. results: the h. forskaolii chloroform extract exhibited very high larvicidal activity after 72 hours of exposure, with lc50 2.0322, 3.89 ...201931118834
characterization of a broad-based mosquito yeast interfering rna larvicide with a conserved target site in mosquito semaphorin-1a genes.rna interference (rnai), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. saccharomyces cerevisiae (baker's yeast) may represent an effective interfering rna expression system that could be used directly for delivery of rna pesticides to mosquito larvae. here we describe characterization of a yeast larvicide developed through bioengineering ...201931118082
[experiences, barriers and facilitators to the implementation of interventions for controlling aedes aegypti in latin america and the caribbean: a qualitative study].this study sought to describe experiences, barriers and facilitators to the implementation of interventions for controlling aedes aegypti in latin america and the caribbean. a qualitative approach with semi-structured in-depth interviews with program implementation experts (19 participants in nine countries) was carried out. we used a software to codify the data and created matrices to compare them. based on our findings, we developed a graphic representation of the theoretical dimensions that e ...201931116251
aedes mosquito infestation in socioeconomically contrasting neighborhoods of panama city.the global expansion and proliferation of aedes aegypti and aedes albopictus represents a growing public health threat due to their capacity to transmit a variety of arboviruses to humans, including dengue, chikungunya, and zika. particularly important in urban regions, where these species have evolved to breed in man-made containers and feed nearly exclusively on human hosts, the threat of vector-borne disease has risen in recent decades due to the growth of cities, progression of climate chang ...201931114946
estimating absolute indoor density of aedes aegypti using removal sampling.quantification of adult aedes aegypti abundance indoors has relied on estimates of relative density (e.g. number of adults per unit of sampling or time), most commonly using traps or timed collections using aspirators. the lack of estimates of the sensitivity of collections and lack of a numerical association between relative and the absolute density of adult ae. aegypti represent a significant gap in vector surveillance. here, we describe the use of sequential removal sampling to estimate absol ...201931113454
use of mosquito repellents to protect against zika virus infection among pregnant women in brazil.to evaluate the use of repellents among pregnant women as a protective measure against infection with the zika virus.201931112836
predicting aquatic development and mortality rates of aedes aegypti.mosquito-borne pathogens continue to be a significant burden within human populations, with aedes aegypti continuing to spread dengue, chikungunya, and zika virus throughout the world. using data from a previously conducted study, a linear regression model was constructed to predict the aquatic development rates based on the average temperature, temperature fluctuation range, and larval density. additional experiments were conducted with different parameters of average temperature and larval den ...201931112566
the ion channel ppk301 controls freshwater egg-laying in the mosquito aedes aegypti.female aedes aegypti mosquitoes are deadly vectors of arboviral pathogens and breed in containers of freshwater associated with human habitation. because high salinity is lethal to offspring, correctly evaluating water purity is a crucial parenting decision. we found that the deg/enac channel ppk301 and sensory neurons expressing ppk301 control egg-laying initiation and choice in ae. aegypti. using calcium imaging, we found that ppk301-expressing cells show ppk301-dependent responses to water bu ...201931112133
juvenile hormone signaling in insect oogenesis.juvenile hormone (jh) plays a crucial role in insect reproduction, but its molecular mode of action only became clear within the last decade. we here review recent findings revealing the intricate crosstalk between jh and ecdysone signaling with nutrient sensing pathways in drosophila melanogaster, aedes aegypti, tribolium castaneum and locusta migratoria. the finding for a critical role of ecdysis triggering hormone (eth) in both molting and ooogenesis now also highlights the importance of an i ...201931109672
transmission potential of african, asian and american zika virus strains by aedes aegypti and culex quinquefasciatus from guadeloupe (french west indies).zika virus (zikv) is an arbovirus that has dramatically spread in south america and the caribbean regions since 2015. the majority of vector incrimination studies available for zikv showed that aedes aegypti mosquitoes are important vectors for this virus. however, several reports suggest that culex quinquefasciatus mosquitoes may be implicated in zikv transmission in certain urban settings. in the present study, we evaluated the vector competence for zikv of cx. quinquefasciatus and ae. aegypti ...201931109248
pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection.wolbachia's ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. wolbachia-infected aedes aegypti are currently being released in locations such as brazil, which regularly experience concurrent outbreaks of different arboviruses. a. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment.201931107912
tire shops in miami-dade county, florida are important producers of vector mosquitoes.human mobility in urban environments is a central part of urbanization and has determined the layout of how cities are projected, built and renovated. one of the most problematic issues of urbanization is how to properly dispose of used tires, considering the worldwide annual production of approximately 1.4 billion units every year. despite the efforts to properly dispose of used tires, they still represent a major problem for public health, notably serving as potential breeding sites for vector ...201931107881
characterization of a yeast interfering rna larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes.new mosquito control strategies are vitally needed to address established and emerging arthropod-borne infectious diseases. here we describe the characterization of a yeast interfering rna larvicide that was developed through the genetic engineering of saccharomyces cerevisiae (baker's yeast) to express a short hairpin rna targeting the aedes aegypti synaptotagmin (aae syt) gene. the larvicide effectively silences the aae syt gene, causes defects at the larval neural synapse, and induces high ra ...201931107878
neurological manifestations of pediatric arboviral infections in the americas.dengue, zika, chikungunya and yellow fever viruses are arboviruses transmitted by the mosquito aedes aegypti. these viruses exhibit marked neurotropism but have rarely been studied. here, we conduct an integrative review of the neurological manifestations caused by these arboviruses in the pediatric population. data on patients under 18 years of age were extracted from literature databases. the most frequently reported neurological manifestations were encephalitis, meningitis, seizures, hypotoni ...201931103802
induction of rna interference to block zika virus replication and transmission in the mosquito aedes aegypti.the yellow fever mosquito, aedes aegypti, serves as the primary vector for epidemic transmission of yellow fever, dengue, zika (zikv), and chikungunya viruses to humans. control of ae. aegypti is currently limited to insecticide applications and larval habitat management; however, to combat growing challenges with insecticide resistance, novel genetic approaches for vector population reduction or transmission interruption are being aggressively pursued. the objectives of this study were to asses ...201931103782
plant essential oils synergize various pyrethroid insecticides and antagonize malathion in aedes aegypti.pyrethroid resistance is a significant threat to agricultural, urban and public health pest control activities. because economic incentives for the production of novel active ingredients for the control of public health pests are lacking, this field is particularly affected by the potential failure of pyrethroid-based insecticides brought about by increasing pyrethroid resistance. as a result, innovative approaches are desperately needed to overcome insecticide resistance, particularly in mosqui ...201931102301
prevalence and distribution of dengue virus in aedes aegypti in yogyakarta city before deployment of wolbachia infected aedes aegypti.indonesia is one of the countries where dengue infection is prevalent. in this study we measure the prevalence and distribution of dengue virus (denv) denv-infected aedes aegypti in yogyakarta city, indonesia, during the wet season when high dengue transmission period occurred, as baseline data before implementation of a wolbachia-infected aedes aegypti trial for dengue control. we applied one-step multiplex real time pcr (rt-pcr) for the type-specific-detection of dengue viruses in field-caught ...201931100967
aedes aegypti saliva impairs m1-associated proinflammatory phenotype without promoting or affecting m2 polarization of murine macrophages.during the feeding process, the mouthparts of hematophagous mosquitoes break the skin barrier and probe the host tissue to find the blood. the saliva inoculated in this microenvironment modulates host hemostasis, inflammation and adaptive immune responses. however, the mechanisms involved in these biological activities remain poorly understood and few studies explored the potential roles of mosquito saliva on the individual cellular components of the immune system. here, we report the immunomodu ...201931097013
enzymatic and molecular characterization of insecticide resistance mechanisms in field populations of aedes aegypti from selangor, malaysia.dengue is a serious public health problem worldwide, including in selangor, malaysia. being an important vector of dengue virus, aedes aegypti are subjected to control measures which rely heavily on the usage of insecticides. evidently, insecticide resistance in ae. aegypti, which arise from several different point mutations within the voltage-gated sodium channel genes, has been documented in many countries. thus, this robust study was conducted in all nine districts of selangor to understand t ...201931097010
re-emergence of chikungunya in the republic of the congo in 2019 associated with a possible vector-host switch.in january 2019, an outbreak of chikungunya virus fever was reported in a rural region near pointe-noire, republic of the congo. molecular and phylogenetic analysis of this new chikv strain demonstrated the presence of the a226v substitution and a surprisingly close relation with aedes aegypti-associated central africa chikungunya strains. these results, combined with the preponderance of aedes albopictus in the outbreak area, suggest a recent vector-host switch facilitated by the emergence and ...201931096054
frequent sugar feeding behavior by aedes aegypti in bamako, mali makes them ideal candidates for control with attractive toxic sugar baits (atsb).current tools and strategies are not sufficient to reliably address threats and outbreaks of arboviruses including zika, dengue, chikungunya, and yellow fever. hence there is a growing public health challenge to identify the best new control tools to use against the vector aedes aegypti. in this study, we investigated ae. aegypti sugar feeding strategies in bamako, mali, to determine if this species can be controlled effectively using attractive toxic sugar baits (atsb).201931206547
the mosquito immune system and the life of dengue virus: what we know and do not know.flaviviruses are largely transmitted to humans by their arthropod vectors such as mosquitoes or ticks. the dengue virus (denv) is one of the members of the family flaviviridae and is the causative agent of dengue fever. in the mosquito vector, denv enters through viremic blood meal and replicates in the mid-gut. newly formed virion particles circulate to various mosquito organs and get transmitted to the next host in subsequent bites. aedes aegypti and aedes albopictus have intricate immune cont ...201931200426
entomo-virological surveillance strategy for dengue, zika and chikungunya arboviruses in field-caught aedes mosquitoes in an endemic urban area of the northeast of brazil.aedes spp. are considered the main vectors of dengue (denv), zika (zikv) and chikungunya (chikv) viruses in the world. arbovirus detection in aedes mosquitoes can alert authorities to possible outbreaks, reducing the impact of these diseases. the purpose of this study was to perform an operational strategy for virological surveillance of denv, zikv and chikv in adult aedes aegypti and aedes albopictus mosquitoes captured at different key-sites in an endemic urban area of the northeast region of ...201931194961
surveys of arboviruses vectors in four cities stretching along a railway transect of burkina faso: risk transmission and insecticide susceptibility status of potential vectors.background: a severe outbreak of dengue occurred in burkina faso in 2016, with the most cases reported in ouagadougou, that highlights the necessity to implement vector surveillance system. this study aims to estimate the risk of arboviruses transmission and the insecticide susceptibility status of potential vectors in four sites in burkina faso. methods: from june to september 2016, house-to-house cross sectional entomological surveys were performed in four cities stretching along a southwest-t ...201931192232
sustained wolbachia-mediated blocking of dengue virus isolates following serial passage in aedes aegypti cell culture.wolbachia is an intracellular endosymbiont of insects that inhibits the replication of a range of pathogens in its arthropod hosts. the release of wolbachia into wild populations of mosquitoes is an innovative biocontrol effort to suppress the transmission of arthropod-borne viruses (arboviruses) to humans, most notably dengue virus. the success of the wolbachia-based approach hinges upon the stable persistence of the 'pathogen blocking' effect, whose mechanistic basis is poorly understood. evid ...201931191980
phylogenetic analysis of dengue-2 serotypes circulating in mangroves in northern cordoba, colombia.in this study, we aimed to identify denv-2 subtypes in aedes aegypti pools collected between 2011 and 2017 in a rural area of northern cordoba, colombia ("la balsa").201931188919
vector competence of aedes aegypti for different strains of zika virus in argentina.the importance of zika virus (zikv) has increased noticeably since the outbreak in the americas in 2015, when the illness was associated with congenital disorders. although there is evidence of sexual transmission of the virus, the mosquito aedes aegypti is believed to be the main vector for transmission to humans. this species of mosquito has not only been found naturally infected with zikv, but also has been the subject of study in many vector competence assays that employ different strains of ...201931188869
optimal control of vaccination in a vector-borne reaction-diffusion model applied to zika virus.zika virus has acquired worldwide concern after a recent outbreak in latin america that started in brazil, with associated neurological conditions such as microcephaly in newborns from infected mothers. the virus is transmitted mainly by aedes aegypti mosquitoes, but direct (sexual) transmission has been documented. we formulate a reaction diffusion model that considers spatial movement of humans and vectors, with local contact transmission of zika virus. vaccination is introduced as a control v ...201931187254
cartography of odor chemicals in the dengue vector mosquito (aedes aegypti l., diptera/culicidae).this study was aimed to identify the chemical compounds of aedes aegypti that can be potentially used to develop pheromone-based vector control methods. in this study, we compared the chemical compounds collected from the organs of mosquitoes at different developmental stages in the life cycle. we also compared the composition and amount of extracts from the different tissues of male and female adult mosquito. interestingly, we found large amount of c17-c20 ethyl and methyl esters in the wings o ...201931186462
male competition and the evolution of mating and life-history traits in experimental populations of aedes aegypti.aedes aegypti is an important disease vector and a major target of reproductive control efforts. we manipulated the opportunity for sexual selection in populations of ae. aegypti by controlling the number of males competing for a single female. populations exposed to higher levels of male competition rapidly evolved higher male competitive mating success relative to populations evolved in the absence of competition, with an evolutionary response visible after only five generations. we also detec ...201931185872
assessing the potential interactions between cellular mirna and arboviral genomic rna in the yellow fever mosquito, aedes aegypti.although the role of exogenous small interfering rna (sirna) and p-element induced wimpy testis (piwi)-interacting rna (pirna) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microrna (mirna). identifying direct interactions between the mosquito mirnas and the rna genome of arboviruses and choosing the relevant mirna candidates to explore resulting antiviral mechanisms are critical. here, we carrie ...201931185697
seasonal and geographic variation in insecticide resistance in aedes aegypti in southern ecuador.insecticide resistance (ir) can undermine efforts to control vectors of public health importance. aedes aegypti is the main vector of resurging diseases in the americas such as yellow fever and dengue, and recently emerging chikungunya and zika fever, which have caused unprecedented epidemics in the region. vector control remains the primary intervention to prevent outbreaks of aedes-transmitted diseases. in many high-risk regions, like southern ecuador, we have limited information on ir. in thi ...201931181073
screening aedes aegypti (diptera: culicidae) populations from pernambuco, brazil for resistance to temephos, diflubenzuron, and cypermethrin and characterization of potential resistance mechanisms.resistance to chemical insecticides detected in aedes aegypti (l.) mosquitoes has been a problem for the national dengue control program (pncd) over the last years. in order to provide deeper knowledge of resistance to xenobiotics, our study evaluated the susceptibility profile of temephos, diflubenzuron, and cypermethrin insecticides in natural mosquito populations from the pernambuco state, associating these results with the local historical use of such compounds. furthermore, mechanisms that ...201931175834
temperature impacts on dengue emergence in the united states: investigating the role of seasonality and climate change.tropical mosquito-borne viruses have been expanding into more temperate regions in recent decades. this is partly due to the coupled effects of temperature on mosquito life history traits and viral infection dynamics and warming surface temperatures, resulting in more suitable conditions for vectors and virus transmission. in this study, we use a deterministic ordinary differential equations model to investigate how seasonal and diurnal temperature fluctuations affect the potential for dengue tr ...201931175008
enhanced vector surveillance to control arbovirus epidemics in colombia.in the wake of the zika epidemic, there has been intensified interest in the surveillance and control of the arbovirus vectors aedes aegypti and aedes albopictus, yet many existing surveillance systems could benefit from improvements. vector control programs are often directed by national governments, but are carried out at the local level, resulting in the discounting of spatial heterogeneities in ecology and epidemiology. furthermore, entomological and epidemiological data are often collected ...201931171922
community assessments for mosquito prevention and control experiences, attitudes, and practices - u.s. virgin islands, 2017 and 2018.aedes aegypti, the mosquito that carries dengue, chikungunya, and zika viruses, is present throughout the u.s. virgin islands (usvi). to reduce mosquitoborne disease transmission, the usvi department of health (vidoh) is responsible for integrated mosquito management. during january 2016-january 2018, usvi experienced its first zika outbreak, with most cases reported during january-december 2016, as well as two category 5 hurricanes (irma on st. thomas/st. john on september 6, 2017, and maria on ...201931170124
the widespread occurrence and potential biological roles of endogenous viral elements in insect genomes.modern genomic sequencing and bioinformatics approaches have detected numerous examples of dna sequences derived from dna and rna virus genomes integrated into both vertebrate and insect genomes. retroviruses encode rna-dependent dna polymerases (reverse transcriptases) and integrases that convert their rna viral genomes into dna proviruses and facilitate proviral dna integration into the host genome. surprisingly, dna sequences derived from rna viruses that do not encode these enzymes also occu ...201931167954
rnaseq analysis of aedes albopictus mosquito midguts after chikungunya virus infection.chikungunya virus (chikv) is an emerging pathogen around the world and causes significant morbidity in patients. a single amino acid mutation in the envelope protein of chikv has led to a shift in vector preference towards aedesalbopictus. while mosquitoes are known to mount an antiviral immune response post-infection, molecular interactions during the course of infection at the tissue level remain largely uncharacterised. we performed whole transcriptome analysis on dissected midguts of aedes a ...201931167461
aedes aegypti microrna, mir-2944b-5p interacts with 3'utr of chikungunya virus and cellular target vps-13 to regulate viral replication.rna interference is among the most important mechanisms that serve to restrict virus replication within mosquitoes, where micrornas (mirnas) are important in regulating viral replication and cellular functions. these mirnas function by binding to complementary sequences mostly in the untranslated regions of the target. chikungunya virus (chikv) genome consists of two open reading frames flanked by 5' and 3' untranslated regions on the two sides. a recent study from our laboratory has shown that ...201931166953
utilizing larvicidal and pupicidal efficacy of eucalyptus and neem oil against aedes mosquito: an approach for mosquito control.plant-based products can provide safe and biodegradable mosquito control agents. the essential oils have a strong odor due to complex secondary metabolites and exhibit lower density than that of water, which renders them suitable to form a thin layer above the water surface. the present study was designed to evaluate the larvicidal, pupicidal activity of eucalyptus and neem oils against aedes aegypti and aedes albopictus.201931161087
wolbachia pipientis occurs in aedes aegypti populations in new mexico and florida, usa.the mosquitoes aedes aegypti (l.) and ae. albopictus skuse are the major vectors of dengue, zika, yellow fever, and chikungunya viruses worldwide. wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. earlier studies have found that wolbachia is commonly detected in ae. albopictus but rarely detected in ae. aegypti. in this study, we used a two-step ...201931161026
molecular evidence of sequential evolution of ddt- and pyrethroid-resistant sodium channel in aedes aegypti.multiple mutations in the voltage-gated sodium channel have been associated with knockdown resistance (kdr) to ddt and pyrethroid insecticides in a major human disease vector aedes aegypti. one mutation, v1016g, confers sodium channel resistance to pyrethroids, but a different substitution in the same position v1016i alone had no effect. in pyrethroid-resistant ae. aegypti populations, v1016i is often linked to another mutation, f1534c, which confers sodium channel resistance only to type i pyre ...201931158225
blood feeding activates the vitellogenic stage of oogenesis in the mosquito aedes aegypti through inhibition of glycogen synthase kinase 3 by the insulin and tor pathways.most mosquitoes, including aedes aegypti, only produce eggs after blood feeding on a vertebrate host. oogenesis in a. aegypti consists of a pre-vitellogenic stage before blood feeding and a vitellogenic stage after blood feeding. primary egg chambers remain developmentally arrested during the pre-vitellogenic stage but complete oogenesis to form mature eggs during the vitellogenic stage. in contrast, the signaling factors that maintain primary egg chambers in pre-vitellogenic arrest or that acti ...201931153832
vapor delivery of plant essential oils alters pyrethroid efficacy and detoxification enzyme activity in mosquitoes.the use of synthetic insecticides to limit the spread of mosquito-borne disease faces a number of significant challenges, including insecticide resistance, concerns related to the environmental impact of widespread insecticide use, as well as slowed development of new insecticide chemistries. one important alternative to broadcast insecticides is the use of personal protection strategies to limit contact with vector species, including the use of spatial repellents that can employ synthetic pyret ...201931153481
transmission of lumpy skin disease virus: a short review.lumpy skin disease (lsd) is a viral transboundary disease endemic throughout africa and of high economic importance that affects cattle and domestic water buffaloes. since 2012, the disease has spread rapidly and widely throughout the middle eastern and balkan regions, southern caucasus and parts of the russian federation. before vaccination campaigns took their full effect, the disease continued spreading from region to region, mainly showing seasonal patterns despite implementing control and e ...201931152757
barriers that limit the implementation of thermal fogging for the control of dengue in colombia: a study of mixed methods.thermal fogging of insecticides is a vector control strategy used by the medellin secretary of health to combat dengue. this method is employed during outbreaks to curb populations of potentially infectious adult mosquitoes and interrupt transmission cycles. while this strategy has been used in medellin since 2007, in some years it has not reduced dengue cases as expected. difficulties in the implementation of fumigation strategies, such as lack of opportunity for treatment and public perception ...201931146722
estimating the impact of city-wide aedes aegypti population control: an observational study in iquitos, peru.during the last 50 years, the geographic range of the mosquito aedes aegypti has increased dramatically, in parallel with a sharp increase in the disease burden from the viruses it transmits, including zika, chikungunya, and dengue. there is a growing consensus that vector control is essential to prevent aedes-borne diseases, even as effective vaccines become available. what remains unclear is how effective vector control is across broad operational scales because the data and the analytical too ...201931145744
effects of constant and fluctuating low temperatures on the development of aedes aegypti (diptera: culicidae) from a temperate region.most studies of the effects of low temperature on the development of immature stages of aedes aegypti (l.) have been performed at constant temperatures in the laboratory, which may not accurately reflect the variable environmental conditions in the field. thus, the aim of this study was to assess the effect of constant temperatures (ct) and fluctuating low temperatures (ft) on the fitness of ae. aegypti of buenos aires, argentina. three ct treatments (12, 14, and 16°c) and three ft treatments (1 ...201931139823
predatory efficacy of five locally available copepods on aedes larvae under laboratory settings: an approach towards bio-control of dengue in sri lanka.many countries are in search of more effective and sustainable methods for controlling dengue vectors, due to undeniable inefficiencies in chemical and mechanical vector control methods. bio-control of vectors by copepods is an ideal method of using interactions in the natural ecosystem for vector management, with minimum consequences on the environment. current study determined the predatory efficacy of five locally abundant copepod species on, aedes larvae under laboratory conditions. copepods ...201931136574
chemical composition and larvicidal activity of the essential oil from the leaves of onychopetalum periquino (rusby) d.m. johnson & n.a. murray.the essential oil (eo) from the leaves of onychopetalum periquino, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (gc-ms), and also was investigated for its larvicidal activity against aedes aegypti larvae. thirteen compounds, representing 91.31% of the crude oil, were identified. major compounds were sesquiterpenes, including β-elemene (53.16%), spathulenol (11.94%) and β-selinene (9.25%). the eo showed high larvicidal activity with a lethal con ...201931135221
sodium ascorbate as a potential toxicant in attractive sugar baits for control of adult mosquitoes (diptera: culicidae) and sand flies (diptera: psychodidae).attractive toxic sugar baits (atsbs) can be an effective vector control tool, especially in areas where aerial or aquatic applications of pesticides are undesirable or impractical. in general, there is a need to develop novel or alternative insecticides for vector control, and there is a demand from consumers for more 'natural' pest control products. sodium ascorbate (sa) is a naturally occurring antioxidant compound, found in fruits and vegetables, and is available commercially in the united st ...201931134270
high infestation of invasive aedes mosquitoes in used tires along the local transport network of panama.the long-distance dispersal of the invasive disease vectors aedes aegypti and aedes albopictus has introduced arthropod-borne viruses into new geographical regions, causing a significant medical and economic burden. the used-tire industry is an effective means of aedes dispersal, yet studies to determine aedes occurrence and the factors influencing their distribution along local transport networks are lacking. to assess infestation along the primary transport network of panama we documented all ...201931133041
the sulcatone receptor of the strict nectar-feeding mosquito toxorhynchites amboinensis.controlling ae. aegypti populations and the prevention of mosquito bites includes the development of monitoring, repelling and attract-and-kill strategies that are based on understanding the chemical ecology of these pests. olfactory-mediated attraction to mammals has recently been linked to the mosquito aedes aegypti odorant receptor or4, which is activated by animal-released 6-methyl-5-hepten-2-one (sulcatone). this odorant is also a major component of flower scents and may play a role outside ...201931129164
a genomic approach to inferring kinship reveals limited intergenerational dispersal in the yellow fever mosquito.understanding past dispersal and breeding events can provide insight into ecology and evolution and can help inform strategies for conservation and the control of pest species. however, parent-offspring dispersal can be difficult to investigate in rare species and in small pest species such as mosquitoes. here, we develop a methodology for estimating parent-offspring dispersal from the spatial distribution of close kin, using pairwise kinship estimates derived from genome-wide single nucleotide ...201931125998
utilization of electrocoagulation for the isolation of alkaloids from the aerial parts of stemona aphylla and their mosquitocidal activities against aedes aegypti.the electrocoagulation (ec) technique is an alternative method of isolating natural products with the advantage of minimizing the amounts of organic solvents required for this process, which are often harmful to the environment. in this research, the ec and the conventional solvent extraction methods were used in the isolation of stemona alkaloids from the aerial parts of stemona aphylla. a comparison was made between the amounts of the isolated alkaloids and the solvents used. the isolated alka ...201931398781
prolonged mosquitocidal activity of siparuna guianensis essential oil encapsulated in chitosan nanoparticles.the use of synthetic insecticides is one of the most common strategies for controlling disease vectors such as mosquitos. however, their overuse can result in serious risks to human health, to the environment, as well as to the selection of insecticidal resistant insect strains. the development of efficient and eco-friendly insect control is urgent, and essential oils have been presented as potential alternatives to synthetic insecticides. moreover, nanoencapsulation techniques can enhance their ...201931398198
forecasting dengue fever in brazil: an assessment of climate conditions.local climate conditions play a major role in the biology of the aedes aegypti mosquito, the main vector responsible for transmitting dengue, zika, chikungunya and yellow fever in urban centers. for this reason, a detailed assessment of periods in which changes in climate conditions affect the number of human cases may improve the timing of vector-control efforts. in this work, we develop new machine-learning algorithms to analyze climate time series and their connection to the occurrence of den ...201931393908
efficacy of spinosad granules and lambda-cyhalothrin contrasts with reduced performance of temephos for control of aedes spp. in vehicle tires in veracruz, mexico.the present study examined the efficacy of λ-cyhalothrin, pyriproxyfen and granular formulations of spinosad and temephos for the control of mosquito larvae present in experimental tires in veracruz state, mexico in the period 2015-2016. both λ-cyhalothrin and spinosad granules provided control of larvae and pupae of aedesaegypti, ae. albopictus and culex spp. in used tires in veracruz state, mexico, over a 9-12 week period, although numbers of culex were low. the numbers of aedes larvae + pupae ...201931390780
the social face of aedes control: women take the floor in a suburban district of fortaleza, brazil.aedes aegypti is currently a critical disease agent and is responsible for viruses such as zika, chikungunya and dengue's four serotypes. this mosquito's relevance to the current social body has come to the fore and triggered urgent ecohealth investigations since this approach aims to articulate different theoretical fields to understand the historical linkages between nature, society and health. based on an ethnographic premise, this study considered the unequal and unfair conditions that make ...201931389545
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