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meta-analysis of aedes aegypti expression datasets: comparing virus infection and blood-fed transcriptomes to identify markers of virus presence.the mosquito aedes aegypti (l.) is vector of several arboviruses including dengue, yellow fever, chikungunya, and more recently zika. previous transcriptomic studies have been performed to elucidate altered pathways in response to viral infection. however, the intrinsic coupling between alimentation and infection were unappreciated in these studies. feeding is required for the initial mosquito contact with the virus and these events are highly dependent. addressing this relationship, we reinterr ...201729376049
mosquito vector-associated microbiota: metabarcoding bacteria and eukaryotic symbionts across habitat types in thailand endemic for dengue and other arthropod-borne diseases.vector-borne diseases are a major health burden, yet factors affecting their spread are only partially understood. for example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. nonetheless, current knowledge of mosquito-associated microbial communities is limited. to characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas ...201829375803
[estimation of underreporting of chikungunya virus infection cases in girardot, colombia, from november, 2014, to may, 2015].chikungunya virus infection in colombia became epidemic in 2015. it is estimated that there is underreporting of cases to the public health surveillance system which can induce bias in epidemiological projections for decision making, a serious problem, as it veils the real magnitude and actual epidemiological importance of this disease.201729373771
the wolbachia strain wau provides highly efficient virus transmission blocking in aedes aegypti.introduced transinfections of the inherited bacteria wolbachia can inhibit transmission of viruses by aedes mosquitoes, and in ae. aegypti are now being deployed for dengue control in a number of countries. only three wolbachia strains from the large number that exist in nature have to date been introduced and characterized in this species. here novel ae. aegypti transinfections were generated using the walba and wau strains. in its native ae. albopictus, walba is maintained at lower density tha ...201829370307
a small-scale investigation into the effect of a larvicidal oil on oviposition site preference by aedes aegypti.larvicidal oils can be used to control immature aedes aegypti and other pestiferous mosquitoes. to test whether oil-based larvicides also act as oviposition deterrents, indoor and semi-field trials were conducted at the navy entomology center of excellence in jacksonville, fl. in both studies, treatment cages consisted of oviposition cups lined with seed germination paper as an oviposition substrate and filled with 1-wk-old southern live oak ( quercus virginiana) leaf litter-infused water. treat ...201729369032
comparison of adult mosquito abundance from biogents-2 sentinel and biogents gravid aedes traps in northeastern florida.we compared the efficacy of the biogents gravid aedes trap (bg-gat) and biogents-2 sentinel (bg2s) trap to assess differences in adult mosquito collection rates between these 2 mosquito surveillance methods in northeastern florida. species diversity of trap collections was significantly greater for bg2s than for bg-gat, with 14 mosquito species collected from the former trap and 4 species from the latter. aedes aegypti and culex quinquefasciatus were the 2 predominant species collected in both t ...201729369031
bg-sentinel™ trap efficacy as a component of proof-of-concept for push-pull control strategy for dengue vector mosquitoes.the efficacy of the bg-sentinel™ (bgs) trap as a "pull" component of a "push-pull" system (pps) for management of the dengue vector, aedes aegypti, was evaluated using local households in pu tuey, kanchanaburi, thailand. the pull component was the concluding phase of a 3-part investigation using a pps combination spatial repellent (sr) and bgs trap to capture adult vector mosquitoes. two sentinel households were selected for evaluation of bgs trap efficacy based on the highest pretrial indoor re ...201729369029
laboratory evaluation of synthetic blends of l-(+)-lactic acid, ammonia, and ketones as potential attractants for aedes aegypti.attraction of aedes aegypti to various binary, trinary, and quaternary blends of lactic acid and ketones with or without ammonia was studied using a dual choice olfactometer. a dose dependent attraction was observed in cases of single compounds where cyclopentanone attracted the highest percentage (36.9 ± 1.8%) of ae. aegypti when tested alone. no significant difference was observed between the attraction levels of trinary and binary blends of lactic acid and acetone or butanone when tested agai ...201729369028
relative insecticidal efficacy of three spatial repellent integrated light sources against aedes aegypti.three repellent products, off! mosquito lamp, insecticandel, and rescue decoshield, were comparatively evaluated against aedes aegypti in 130-m2 enclosed areas with a 317-m3 air volume. the results showed that the off! mosquito lamp with metofluthrin had a greater effect than the insecticandel with transfluthrin, which had greater effect than the decoshield with lemongrass oil and several other plant oils against ae. aegypti. the off! mosquito lamp was the only product to exceed 50% mortality. a ...201729369020
multifaceted functional implications of an endogenously expressed trna fragment in the vector mosquito aedes aegypti.the mosquito aedes aegypti is the primary vector of human arboviral diseases caused by dengue, chikungunya and zika viruses. many studies have shown the potential roles of small rna molecules such as microrna, small interfering rna and piwi-interacting rna in vector mosquitoes. the function of trna fragments (trf), the newly discovered class of small rnas, in mosquitoes is not known. in this study, we show that specific trfs are expressed in significantly differential manner between males and fe ...201829364883
larvicidal activity against aedes aegypti and chemical characterization of the inflorescences of tagetes patula.the crude acetone extract (cae) of defatted inflorescences of tagetes patula was partitioned into five semipurified fractions: n-hexane (hf), dichloromethane (df), ethyl acetate (eaf), n-butanol (bf), and aqueous (aqf). bf was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to hsccc, where patuletin and patulitrin were isolated. cae and the fractions bf, eaf, df, and aqf were analyzed by lc-dad-ms, and patuletin and patulitrin were ...201729362590
[dengue, zika, chikungunya and the development of vaccines].dengue (denv), zika (zikv) and chikungunya (chikv), three arbovirosis transmitted by aedes mosquitoes, have spread in recent decades in humid tropical and subtropical zones. dengue is epidemic in subtropical areas of argentina. denv infection confers lasting immunity against the infecting serotype but increases the risk of serious disease upon reinfection by any of the other three. the recombinant tetravalent vaccine dengvaxia® prevents severe dengue and hospitalization in seropositive subjects. ...201829360072
cytoplasmic glutamine synthetase gene expression regulates larval development in bactrocera dorsalis (hendel).in insects, glutamine synthetase (gs), a key enzyme in the synthesis of glutamine, has been reported to be associated with embryonic development, heat shock response, and fecundity regulation. however, little is known about the influence of gs on postembryonic development. in this study, we demonstrate that blocking the activity of gs in the oriental fruit fly (bactrocera dorsalis) with use of a gs-specific inhibitor (l-methionine s-sulfoximine), led to a significant delay in larval development, ...201829359358
persistence of toxic activity of fermentation extracts from bacillus thuringiensis var. israelensis after more than three decades of storage.this study was carried out to determine the persistence of toxicity of fermentation extracts of bacillus thuringiensis var. israelensis after more than three decades of storage. for this purpose, a population of aedes aegypti was established. the mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified who protocol. the extracts with the highest mortality rate were determined in triplicat ...201729358951
fine-scale landscape genomics helps explain the slow spatial spread of wolbachia through the aedes aegypti population in cairns, australia.the endosymbiotic bacterium wolbachia suppresses the capacity for arbovirus transmission in the mosquito aedes aegypti, and can spread spatially through wild mosquito populations following local introductions. recent introductions in cairns, australia have demonstrated slower than expected spatial spread. potential reasons for this include: (i) barriers to ae. aegypti dispersal; (ii) higher incidence of long-range dispersal; and (iii) intergenerational loss of wolbachia. we investigated these th ...201829358725
current concerns and perspectives on zika virus co-infection with arboviruses and hiv.dissemination of vector-borne viruses, such as zika virus (zikv), in tropical and sub-tropical regions has a complicated impact on the immunopathogenesis of other endemic viruses such as dengue virus (denv), chikungunya virus (chikv) and human immunodeficiency virus (hiv). the consequences of the possible co-infections with these viruses have specifically shown significant impact on the treatment and vaccination strategies. zikv is a mosquito-borne flavivirus from african and asian lineages that ...201829352633
aerial ulv control of aedes aegypti with naled (dibrom) inside simulated rural village and urban cryptic habitats.we conducted aerial fixed wing ultra low volume (ulv) spray trials with naled to investigate penetration of exposed and simulated cryptic habitat within opened buildings, partially sealed buildings, and outdoor locations targeting sentinel adult aedes aegypti mosquitoes in north central florida. mortality was observed in open and closed buildings and outdoors, even in mosquitoes placed in cryptic habitats. observations on the impact of building type, mosquito exposure method such as placement in ...201829352307
reduction in musca domestica fecundity by dsrna-mediated gene knockdown.house flies (musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the u.s. non-target effects and widespread resistance challenge the efficacy of traditional chemical control. double stranded rna (dsrna) has been suggested as a biopesticide for m. domestica but a phenotypic response due to the induction of the rnai pathway has not been demonstrated in adults. in this study female house flies were injected with dsrna targeting actin-5c or ribo ...201829342168
zika virus in salivary glands of five different species of wild-caught mosquitoes from mexico.zika virus (zikv) is a mosquito-borne pathogen, and aedes aegypti has been identified as the main vector of the disease. other mosquito species in the aedes and culex genera have been suggested to have the potential for being competent vectors based on experimental exposition of mosquitoes to an infectious blood meal containing zikv. here, we report the isolation in cell culture of zikv obtained from different body parts of wild-caught female mosquitoes (ae. aegypti, ae. vexans, cx. quinquefasci ...201829339746
wolbachia-induced transcription factor gata4 suppresses ovary-specific genes blastoderm-specific protein 25d and imaginal disc growth factor.the endosymbiotic bacterium wolbachia infects a wide array of insect hosts and has been implicated in a range of biological modifications as a consequence of its infection. previously, it was shown that the transcription factor gata4 was significantly induced in wolbachia wmelpop-cla strain infected aedes aegypti whole mosquitoes and cells. here, we provide evidence that this induction also occurs in mosquito ovaries where the ovary-specific genes blastoderm-specific protein 25d (bsg25d) and ima ...201829336504
vibrational properties of bulk boric acid 2a and 3t polymorphs and their two-dimensional layers: measurements and density functional theory calculations.boric acid (h3bo3) is being used effectively nowadays in traps/baits for the management of aedes aegypti l. and aedes albopictus skuse species of mosquitoes, which are the main spreading vectors worldwide for diseases such as malaria, dengue, and zika. previously, we published results on the structural, electronic, and optical properties of its molecular triclinic h3bo3-2a and trigonal h3bo3-3t polymorphs within the framework of density functional theory (dft). because of the renewed importance ...201829328646
genome sequence of a novel strain of a phasi charoen-like virus identified in zhanjiang, china.here, we report the genome sequence of a novel strain of a phasi charoen-like virus, isolated from aedes aegypti mosquitoes caught in zhanjiang province of china. phylogenetic analysis suggested that this virus belongs to a new genus, goukovirus, in the family bunyaviridae this is the first reported genome sequence of a phasi charoen-like virus identified in china.201829326198
the discovery of arthropod-specific viruses in hematophagous arthropods: an open door to understanding the mechanisms of arbovirus and arthropod evolution?the discovery of an odd virus from hematophagous arthropods 40 years ago by stollar and thomas described cell fusing agent virus in cells derived from aedes aegypti mosquitoes. then came the report of kamiti river virus from ae. macintoshi in 1999, followed by worldwide reports of the discovery of other viruses of mosquitoes, ticks, and midges that replicate only in arthropods and not in vertebrates or in vertebrate cells. these viruses (now totaling at least 64 published) have genomes analogous ...201829324047
citrus seed oils efficacy against larvae of aedes aegypti.dengue fever is a serious public health issue in pakistan for many years. globally plants have been reported to contain compounds with insecticidal properties. these properties have been demonstrated more recently on the larval stages of mosquitoes. therefore, citrus cultivar seeds were evaluated for larvicidal potential against the primary dengue vector aedes aegypti.201729322059
dengue hemorrhagic fever virus in saudi arabia: a review.dengue fever is a global disease with a spectrum of clinical manifestation ranging from mild febrile disease to a severe disease in the form of dengue hemorrhagic fever and dengue shock syndrome. dengue virus is one viral hemorrhagic fever that exists in the kingdom of saudi arabia in addition to alkhurma (alkhurma) hemorrhagic fever, chikungunya virus, crimean-congo hemorrhagic fever, and rift valley fever. the disease is limited to the western and south-western regions of saudi arabia, where a ...201829319426
qsar analysis of plant-derived compounds with larvicidal activity against zika aedes aegypti (diptera: culicidae) vector using freely available descriptors.we develop a qsar model for predicting the larvicidal activity of 60 plant-derived molecules against aedes aegypti l. (diptera: culicidae), vector of several diseases such as, dengue, yellow fever, chikungunya and zika virus. the balanced subsets method (bsm) based on k-means cluster analysis (k-mca) is employed to split the data set. the replacement method (rm) variable subset selection technique coupled with multivariable linear regression (mlr) proves to be successful for exploring 18,326 mol ...201829314584
krüppel homologue 1 acts as a repressor and an activator in the transcriptional response to juvenile hormone in adult mosquitoes.krüppel homologue 1 (kr-h1) is a zinc finger transcription factor that is upregulated in insects by juvenile hormone (jh) in metamorphosis and adult reproduction. the molecular function of kr-h1 in reproduction remains largely unknown. here we report that aakr-h1 functions as an important transcription regulator in adult female aedes aegypti mosquitoes. the amount of aakr-h1 protein increases with rising jh levels after adult emergence, reaches its peak at 48 h after eclosion, then decreases gra ...201829314423
aristololactam derivatives from the fruits of aristolochia contorta bunge.three new aristololactam derivatives, aristololactam w-y (1-3), and three known compounds (4-6) were isolated from the fruits of aristolochia contorta bunge. compounds 1 and 2 represent the first example of an n-ch2och3 aristololactam derivative from natural products. their structures were elucidated by 1d/2d nmr and hresims spectra. all of the isolated compounds were evaluated for their insecticidal activity against 4th instar larvae of aedes aegypti. compound 4 displayed insecticidal activity ...201829313366
the genomes of four meyerozymacaribbica isolates and novel insights into the meyerozymaguilliermondii species complex.yeasts of the meyerozyma guilliermondii species complex are widespread in nature and can be isolated from a variety of sources, from the environment to arthropods to hospital patients. to date, the species complex comprises the thoroughly studied and versatile m. guilliermondii, and the hard to distinguish m. caribbica, and candida carpophila here we report the whole-genome sequencing and de novo assembly of four m. caribbica isolates, identified with the most recent molecular techniques, derive ...201829311113
critical low temperature for the survival of aedes aegypti in taiwan.taiwan is geographically located in a region that spans both tropical and subtropical climates (22-25°n and 120-122°e). the taiwan centers for disease control have found that the ecological habitat of aedes aegypti appears only south of 23.5°n. low temperatures may contribute to this particular habitat distribution of ae. aegypti under the influence of the east asian winter monsoon. however, the threshold condition related to critically low temperatures remains unclear because of the lack of lar ...201829310716
synergistic effect of fertilizer and plant material combinations on the development of aedes aegypti (diptera: culicidae) and anopheles gambiae (diptera: culicidae) mosquitoes.chemical fertilizers are used everywhere, which often pollute the breeding sites of mosquitoes. in this laboratory study, the consequences on aedes aegypti (l.) (diptera: culicidae) and anopheles gambiae (giles) (diptera: culicidae) of water-containing plant matter (pm) alone, or in association with an npk type of fertilizer (pm+npk), were evaluated. to obtain a 20% imaginal emergence of an. gambiae (iet20), the bioassays carried out with pm have evidenced that its larvae need four times as much ...201729309617
assessment of a trap based aedes aegypti surveillance program using mathematical modeling.the goal of this study was to assess the goodness-of-fit of theoretical models of population dynamics of aedes aegypti to trap data collected by a long term entomological surveillance program. the carrying capacity k of this vector was estimated at city and neighborhood level. adult mosquito abundance was measured via adults collected weekly by a network of sticky traps (mosquitraps) from january 2008 to december 2011 in vitória, espírito santo, brazil. k was the only free parameter estimated by ...201829304070
hypoxia-induced transcription factor signaling is essential for larval growth of the mosquito aedes aegypti.gut microbes positively affect the physiology of many animals, but the molecular mechanisms underlying these benefits remain poorly understood. we recently reported that bacteria-induced gut hypoxia functions as a signal for growth and molting of the mosquito aedes aegypti in this study, we tested the hypothesis that transduction of a gut hypoxia signal requires hypoxia-induced transcription factors (hifs). expression studies showed that hif-α was stabilized in larvae containing bacteria that in ...201829298915
group b wolbachia strain-dependent inhibition of arboviruses.mosquito-borne viruses, including zika virus (zikv) and dengue virus (denv), are global threats that continue to infect millions annually. historically, efforts to combat the spread of these diseases have sought to eradicate the mosquito population. this has had limited success. recent efforts to combat the spread of these diseases have targeted the mosquito population and the mosquito's ability to transmit viruses by altering the mosquito's microbiome. the introduction of particular strains of ...201829297702
zika virus infection modulates the bacterial diversity associated with aedes aegypti as revealed by metagenomic analysis.zika is a re-emerging infection that has been considered a major threat to global public health. currently at least 100 countries are at risk of zika virus (zikv) transmission. aedes aegypti is the main mosquito vector in the americas. this vector is exposed to, and interacts symbiotically with a variety of microorganisms in its environment, which may result in the formation of a lifetime association. here, the unknown effect that zikv exerts on the dynamic bacterial community harbored by this m ...201829293631
insecticide treated curtains and residual insecticide treatment to control aedes aegypti: an acceptability study in santiago de cuba.within the context of a field trial conducted by the cuban vector control program (aacp), we assessed acceptability of insecticide-treated curtains (itcs) and residual insecticide treatment (rit) with deltamethrin by the community. we also assessed the potential influence of interviewees' risk perceptions for getting dengue and disease severity.201829293501
n-substituted methyl maleamates as larvicidal compounds against aedes aegypti (diptera: culicidae).severe human arboviral diseases can be transmitted by the mosquito aedes aegypti (l.), including dengue, chikungunya, zika, and yellow fever. the use of larvicides in containers that can result as potential breeding places and cannot be eliminated is the main alternative in control programs. however, their continuous and widespread use caused an increase in insecticide-resistant populations of this mosquito. the aim of this study was to evaluate the effect of three n-substituted methyl maleamate ...201829290037
dengue and yellow fever virus vectors: seasonal abundance, diversity and resting preferences in three kenyan cities.the transmission patterns of dengue (denv) and yellow fever (yfv) viruses, especially in urban settings, are influenced by aedes (stegomyia) mosquito abundance and behavior. despite recurrent dengue outbreaks on the kenyan coast, these parameters remain poorly defined in this and other areas of contrasting dengue endemicity in kenya. in assessing the transmission risk of denv/yfv in three kenyan cities, we determined adult abundance and resting habits of potential aedes (stegomyia) vectors in ki ...201729284522
genetically modified aedes aegypti to control dengue: a review.dengue is an acute infectious disease of viral etiology characterized by lymphadenopathy, leucopenia, headache, biphasic fever, pain in various parts of the body, rashes, and extreme physical weakness. it is a vector-borne disease caused by a positive-stranded rna virus of the family flaviviridae, genus flavivirus. dengue inflicts a significant health, economic, and social burden on populations of endemic areas. dengue virus is transmitted to humans by the mosquito vector aedes aegypti. vaccines ...201729283327
family level variation in wolbachia-mediated dengue virus blocking in aedes aegypti.the mosquito vector aedes aegypti is responsible for transmitting a range of arboviruses including dengue (denv) and zika (zikv). the global reach of these viruses is increasing due to an expansion of the mosquito's geographic range and increasing urbanization and human travel. vector control remains the primary means for limiting these diseases. wolbachia pipientis is an endosymbiotic bacterium of insects that has the ability to block the replication of pathogens, including flaviviruses such as ...201729282144
african and asian zika virus isolates display phenotypic differences both in vitro and in vivo.zika virus (zikv) is a mosquito-borne member of the genus flavivirus that has emerged since 2007 to cause outbreaks in africa, asia, oceania, and most recently, in the americas. here, we used an isolate history as well as genetic and phylogenetic analyses to characterize three low-passage isolates representing african (ard 41525) and asian (cpc-0740, sv0127-14) lineages to investigate the potential phenotypic differences in vitro and in vivo. the african isolate displayed a large plaque phenotyp ...201729280428
field- and clinically derived estimates of wolbachia-mediated blocking of dengue virus transmission potential in aedes aegypti mosquitoes.the wmel strain of wolbachia can reduce the permissiveness of aedes aegypti mosquitoes to disseminated arboviral infections. here, we report that wmel-infected ae. aegypti (ho chi minh city background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (denv) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. the wmel-infected mosquitoes that are field-reared have even greater relative resistance to denv infection ...201829279375
altitudinal distribution of aedes indices during dry season in the dengue endemic area of central java, indonesiaaedes mosquitoes, mainly aedes aegypti and aedes albopictus, are the primary and secondary vectors of dengue viruses in indonesia, with transmission occurring by sucking blood. the density of the vectors is influenced by season and rainfall, but limited by altitude. the aim of the study is to describe the density and distribution of dengue vectors during the dry season based on the altitudes of recent dengue cases in five regencies of central java province, indonesia. mosquito larvae and pupae w ...201729274215
zika virus: an emerging infectious disease with serious perinatal and neurologic complications.zika virus (zikv) is a flavivirus that is primarily transmitted by aedes aegypti, the mosquito vector also important in the transmission of the flaviviruses responsible for dengue fever, yellow fever and chikungunya. due to occurrence in the same geographic regions, serological cross-reactivity, and similar, albeit often less severe clinical manifestations as dengue and chikungunya infections, zikv infection likely went undetected and/or misdiagnosed for many years. zikv is somewhat unique among ...201729273403
history and emergence of zika virus.zika virus was discovered in east africa in 1947 by the rockefeller foundation during investigations on the ecology of yellow fever. although it was subsequently shown to have widespread distribution in africa and asia, it was not known to cause epidemics until 2007. this paper describes the history of the virus discovery, emergence and evolution as an epidemic virus, and the its evolving clinical spectrum.201729267917
zika virus mosquito vectors: competence, biology, and vector control.zika virus (zikv) (flaviviridae, flavivirus) has become one of the most medically important mosquito-borne viruses because of its ability to cause microcephaly in utero and guillain-barré syndrome in adults. this virus emerged from its sylvatic cycle in africa to cause an outbreak in yap, federated states of micronesia in 2007, french polynesia in 2014, and most recently south america in 2015. the rapid expansion of zikv in the americas largely has been due to the biology and behavior of its vec ...201729267910
at the origin of a worldwide invasion: unraveling the genetic makeup of the caribbean bridgehead populations of the dengue vector aedes aegypti.human-driven global environmental changes have considerably increased the risk of biological invasions, especially the spread of human parasites and their vectors. among exotic species that have major impacts on public health, the dengue fever mosquito aedes aegypti originating from africa has spread worldwide during the last three centuries. although considerable progress has been recently made in understanding the history of this invasion, the respective roles of human and abiotic factors in s ...201829267872
chikungunya: molecular aspects, clinical outcomes and pathogenesis.the alarming worldwide emergence of the chikungunya virus began in the last decade. since the first autochthonous transmission in mexico in november of 2014, the virus has spread throughout the country, resulted in multiple outbreaks. this virus produces an acute and self-limiting disease characterized by fever, polyarthralgia, myalgia, exanthema, and general malaise. it is transmitted to humans by the bite of aedes aegypti and a. albopictus mosquitoes. the fact that the clinical presentation is ...201729265115
characteristics of aedes aegypti adult mosquitoes in rural and urban areas of western and coastal kenya.aedes aegypti is the main vector for yellow fever, dengue, chikungunya and zika viruses. recent outbreaks of dengue and chikungunya have been reported in kenya. presence and abundance of this vector is associated with the risk for the occurrence and transmission of these diseases. this study aimed to characterize the presence and abundance of ae. aegypti adult mosquitoes from rural and urban sites in western and coastal regions of kenya. presence and abundance of ae. aegypti adult mosquitoes wer ...201729261766
individual co-variation between viral rna load and gene expression reveals novel host factors during early dengue virus infection of the aedes aegypti midgut.dengue virus (denv) causes more human infections than any other mosquito-borne virus. the current lack of antiviral strategies has prompted genome-wide screens for host genes that are required for denv infectivity. earlier transcriptomic studies that identified denv host factors in the primary vector aedes aegypti used inbred laboratory colonies and/or pools of mosquitoes that erase individual variation. here, we performed transcriptome sequencing on individual midguts in a field-derived ae. aeg ...201729261661
assessment of maldi-tof mass spectrometry for filariae detection in aedes aegypti mosquitoes.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is an emerging tool for routine identification of bacteria, archaea and fungi. it has also been recently applied as an accurate approach for arthropod identification. preliminary studies have shown that the maldi-tof ms was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and plasmodium parasites, respectively. the aim of the present study was to test the efficien ...201729261659
origin of a high-latitude population of aedes aegypti in washington, dc.an overwintering population of aedes aegypti has been documented in the capitol hill neighborhood of washington, dc, since 2011. mitochondrial cytochrome oxidase i (mtcoi) sequence data presented in a previous study traced the origin to the new world. here, we use microsatellite and snp chip data along with more mitochondrial dna (mtdna) sequences on washington ae. aegypti samples and samples from potential sources to further narrow the origin of this population. genetically, washington ae. aegy ...201729260658
from ground pools to treeholes: convergent evolution of habitat and phenotype in aedes mosquitoes.invasive mosquito species are responsible for millions of vector-borne disease cases annually. the global invasive success of aedes mosquitoes such as aedes aegypti and aedes albopictus has relied on the human transport of immature stages in container habitats. however, despite the importance of these mosquitoes and this ecological specialization to their widespread dispersal, evolution of habitat specialization in this group has remained largely unstudied. we use comparative methods to evaluate ...201729258425
zika virus exhibits lineage-specific phenotypes in cell culture, in aedes aegypti mosquitoes, and in an embryo model.zika virus (zikv) has quietly circulated in africa and southeast asia for the past 65 years. however, the recent zikv epidemic in the americas propelled this mosquito-borne virus to the forefront of flavivirus research. based on historical evidence, zikv infections in africa were sporadic and caused mild symptoms such as fever, skin rash, and general malaise. in contrast, recent asian-lineage zikv infections in the pacific islands and the americas are linked to birth defects and neurological dis ...201729258204
seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan.to find out the variations in larval and adult density of aedes aegypti in different seasons under different ecological conditions.201729256519
social-ecological factors and preventive actions decrease the risk of dengue infection at the household-level: results from a prospective dengue surveillance study in machala, ecuador.in ecuador, dengue virus (denv) infections transmitted by the aedes aegypti mosquito are among the greatest public health concerns in urban coastal communities. community- and household-level vector control is the principal means of controlling disease outbreaks. this study aimed to assess the impact of knowledge, attitudes, and practices (kaps) and social-ecological factors on the presence or absence of denv infections in the household.201729253873
an approach of the exact linearization techniques to analysis of population dynamics of the mosquito aedes aegypti.dengue fever, chikungunya and zika are caused by different viruses and mainly transmitted by aedes aegypti mosquitoes. these diseases have received special attention of public health officials due to the large number of infected people in tropical and subtropical countries and the possible sequels that those diseases can cause. in severe cases, the infection can have devastating effects, affecting the central nervous system, muscles, brain and respiratory system, often resulting in death. vaccin ...201729253493
aedes aegypti resistance development to commonly used insecticides in jakarta, indonesia.aedes aegypti is the primary vector of various relevant arthropod-borne viral infectious diseases worldwide. the mosquito control is still mainly performed by using insecticides but their effectiveness is increasingly questioned nowadays. we here conducted a study on ae. aegypti resistance development towards several commonly used insecticides in the capital city of jakarta, indonesia. in order to achieve this goal, ae. aegypti eggs from jakarta were collected with ovitraps and hatched in the in ...201729253003
larvicidal potential of juglans regia male flower against anopheles stephensi, aedes aegypti and culex quinquefasciatus.the diseases vectored by mosquitoes continue to be a main cause of illnesses and death throughout the world. the methanol extract of juglans regia male flower was screened for larvicidal activity against three therapeutically important mosquito vectors viz., malarial vector, anopheles stephensi; dengue vector, aedes aegypti and the filarial vector, culex quinquefasciatus. the larvicidal activity was assayed against the early fourth-instar larvae of tested mosquito species at a concentration rang ...201729251995
green synthesis of silver nanoparticles using holarrhena antidysenterica (l.) wall.bark extract and their larvicidal activity against dengue and filariasis vectors.the present study was carried out to evaluate the larvicidal potential of methanol, hexane, acetone, chloroform, and aqueous bark extracts of holarrhena antidysenterica (l.) wall. and silver nanoparticles (agnps) synthesized using aqueous bark extract against the third instar larvae of aedes aegypti l. and culex quinquefasciatus say. agnps were prepared by adding 10 ml of aqueous bark extract in 90 ml of 1 mm silver nitrate (agno3) solution. after 5 min of mixing, a change in color from yellow t ...201829250727
aedes aegypti microrna mir-2b regulates ubiquitin-related modifier to control chikungunya virus replication.arboviruses that replicate in mosquitoes activate innate immune response within mosquitoes. regulatory non-coding micrornas (mirna) are known to be modulated in mosquitoes during chikungunya infection. however, information about targets of these mirnas is scant. the present study was aimed to identify and analyze targets of mirnas that are regulated during chikungunya virus (chikv) replication in aedes aegypti cells and in the mosquito. employing next-generation sequencing technologies, we ident ...201729247247
zika virus can be venereally transmitted between aedes aegypti mosquitoes.alternative transmission routes have been described for zika virus (zikv). here, we assessed for the first time the venereal transmission of zikv between aedes aegypti under laboratory conditions.201729246237
the attractiveness of the gravid aedes trap to dengue vectors in fiji.the gravid aedes trap (gat) is a passive trap that relies on visual and olfactory cues to lure and capture gravid mosquitoes. the gat was designed as a dengue vector surveillance tool for use in conditions where power sources are not readily available. experiments were conducted over a 10-d period in fiji to determine which species of mosquitoes the gat would collect, how different infusion types affect the attractiveness of the trap, how long each infusion type took to begin attracting mosquito ...201729244174
nanotitania crystals induced efficient photocatalytic color degradation, antimicrobial and larvicidal activity.textile industries release tonnes of harmful toxic dyes into the environment, causing severe effects on living organisms, including humans. mosquitoes vectors spread important diseases which cause millions of human deaths worldwide. to control mosquitoes a number of synthetic mosquitocidal agents have been employed but all these pesticides pose harmful effects to human health and non-target species and also led to resistance development in treated vectors. microbial strains are also developing r ...201829241121
analytical report of the 2016 dengue outbreak in córdoba city, argentina.after elimination of the aedes aegypti vector in south america in the 1960s, dengue outbreaks started to reoccur during the 1990s; strongly in argentina since 1998. in 2016, córdoba city had the largest dengue outbreak in its history. in this article we report this outbreak including spatio-temporal analysis of cases and vectors in the city. a total of 653 dengue cases were recorded by the laboratory-based dengue surveillance system and georeferenced by their residential addresses. case maps wer ...201729239555
assessing natural infection with zika virus in the southern house mosquito, culex quinquefasciatus, during 2016 in puerto rico.the epidemic of zika in the western hemisphere has led to intense investigations of all species important in the transmission of zika virus (zikv), including putative mosquito vectors. although evidence points to stegomyia (= aedes) (diptera: culicidae) mosquitoes as the primary vectors in nature among humans, there remains the possibility that other common mosquito species may be implicated in the rapid spread of the virus. herein, field-caught culex quinquefasciatus (diptera: culicidae) collec ...201729239003
aedes aegypti breeding site in an underground rainwater reservoir: a warning.we describe the discovery of aedes aegypti underground breeding site in the pinheiros neighborhood of são paulo, sp, during an entomological survey program performed in 2016. even with intense surveillance and vector control, large numbers of mosquitoes were present in this area. a detailed investigation allowed for the detection of ae. aegypti in an underground reservoir used for rainwater storage. after the implementation of protection screens in the accesses, the presence of the vector was no ...201729236877
detection of zika virus in aedes mosquitoes from mexico.we report on the results of an entomovirological surveillance system of aedes populations performed by the ministry of health of the central state of san luis potosí, mexico.201729232453
efficacy of 13 commercial household aerosol insecticides against aedes aegypti (diptera: culicidae) from morelos, mexico.in mexico, aedes aegypti (l.) (diptera: culicidae) is the primary vector of dengue, zika, and chikungunya viruses. control programs include community participation using personal protection such as household aerosol insecticides. in both, urban or rural areas, the use of aerosol insecticides is a common practice to avoiding mosquito biting. thus, information on the efficacy of commercial products must be available. this study reports the efficacy of 13 household aerosol insecticides against ae. ...201729228197
in silico validation of d7 salivary protein-derived b- and t-cell epitopes of aedes aegypti as potential vaccine to prevent transmission of flaviviruses and togaviruses to humans.mosquito (aedes aegyptii) salivary proteins play a crucial role in facilitating viral transmission from vector-to-host due to their role in facilitating the "blood meal" of the vector. three main proteins, d7, aegyptin and sialokinin play a role in this process. using in-silico programs, we identified b- and t-cell epitopes in the mosquito salivary proteins d7 long and short form. t-cell epitopes with high affinity to the most prevalent hla mhc class-i supertypes among different population group ...201729225429
species specific rna a-to-i editing of mosquito rdl modulates gaba potency and influences agonistic, potentiating and antagonistic actions of ivermectin.the insect gaba receptor, rdl, is the target of several classes of pesticides. the peptide sequences of rdl are generally highly conserved between diverse insects. however, rna a-to-i editing can effectively alter amino acid residues of rdl in a species specific manner, which can affect the potency of gaba and possibly insecticides. we report here that rna a-to-i editing alters the gene products of rdl in three mosquito disease vectors, recoding five amino acid residues in rdl of aedes aegypti a ...201729223796
the first detected airline introductions of yellow fever mosquitoes (aedes aegypti) to europe, at schiphol international airport, the netherlands.air-borne introduction of exotic mosquitoes to schiphol airport in the netherlands has been considered plausible based upon findings of mosquitoes in aircraft cabins during 2008, 2010 and 2011. beginning in 2013, surveillance efforts at schiphol had focused on promptly detecting accidental introductions at the airport facilities in order to quickly react and avoid temporary proliferation or establishment of mosquito populations, identify the origin of the introductions, and avoid potential trans ...201729221490
full-length genome sequence of a chikungunya virus isolate from the 2017 autochthonous outbreak, lazio region, italy.we report here the genome sequence of a human chikungunya virus isolate from the ongoing autochthonous outbreak in central italy. the sequence (east-central-south african lineage, indian ocean sublineage), which is similar to recent sequences from pakistan and india, shows e1 and e2 signatures of strains whose main mosquito vector is aedes aegypti, although aedes albopictus is the vector in italy.201729217791
the gastric caecum of larval aedes aegypti: stimulation of epithelial ion transport by 5-hydroxytryptamine and camp.we report measurements of ion transport across the gastric caecum of larvae of aedes aegypti, a vector of yellow fever that inhabits a variety of aquatic habitats ranging from freshwater to brackish water. we provide the first measurements of the effect of 5-hydroxytryptamine (5-ht) on transepithelial potential (tep), luminal ion concentrations and electrochemical potentials, as well as basolateral membrane potential and h+, na+ and k+ fluxes. tep, basolateral membrane potential, and h+, k+, and ...201729217627
the immune strategies of mosquito aedes aegypti against microbial infection.yellow fever mosquito aedes aegypti transmits many devastating arthropod-borne viruses (arboviruses), such as dengue virus, yellow fever virus, chikungunya virus, and zika virus, which cause great concern to human health. mosquito control is an effective method to block the spread of infectious diseases. ae. aegypti uses its innate immune system to fight against arboviruses, parasites, and fungi. in this review, we briefly summarize the recent findings in the immune response of ae. aegypti again ...201729217264
differences in prevalence of symptomatic zika virus infection by age and sex-puerto rico, 2016.during the zika virus (zikv) outbreak in puerto rico in 2016, non-pregnant women aged 20-39 years were disproportionately identified with zikv disease. we used household-based cluster investigations to determine if this disparity was associated with age- or sex-dependent differences in the rate of zikv infection or reporting symptoms.201729216376
novel wolbachia-transinfected aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes.wolbachia pipientis from drosophila melanogaster (wmel) is an endosymbiotic bacterium that restricts transmission of human pathogenic flaviviruses and alphaviruses, including dengue, zika, and chikungunya viruses, when introduced into the mosquito vector aedes aegypti. to date, wmel-infected ae. aegypti have been released in field trials in 5 countries to evaluate the effectiveness of this strategy for disease control. despite the success in establishing wmel-infected mosquitoes in wild populati ...201729216317
dengue serotype-specific immune response in aedes aegypti and aedes albopictus.dengue viruses (denv) are considered one of the most important emerging pathogens and dengue disease is a global health threat. the geographic expansion of dengue viruses has led to co-circulation of all four dengue serotypes making it imperative that new denv control strategies be devised.201729211244
an aedes aegypti-associated fungus increases susceptibility to dengue virus by modulating gut trypsin activity.transmission of dengue virus (denv) requires successful completion of the infection cycle in the aedes aegypti vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. while certain ae. aegypti midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility to denv infection. we show that a talaromyces (tsp_pr) fungus, isolated from field-caught ae. aegypti, ...201729205153
alternative patterns of sex chromosome differentiation in aedes aegypti (l).some populations of west african aedes aegypti, the dengue and zika vector, are reproductively incompatible; our earlier study showed that divergence and rearrangements of genes on chromosome 1, which bears the sex locus (m), may be involved. we also previously described a proposed cryptic subspecies senaae (pk10, senegal) that had many more high inter-sex fst genes on chromosome 1 than did ae.aegypti aegypti (aaa, pai lom, thailand). the current work more thoroughly explores the significance of ...201729202694
dengue fever outbreaks in eritrea, 2005-2015: a case for strengthening surveillance, control and reporting.the geographic distribution and burden of dengue is increasing globally. this study aims to evaluate dengue outbreaks and to substantiate the need for strengthened surveillance, reporting and control in eritrea.201629202065
global transcriptome analysis of aedes aegypti mosquitoes in response to zika virus infection.zika virus (zikv) of the flaviviridae family is a recently emerged mosquito-borne virus that has been implicated in the surge of the number of microcephaly instances in south america. the recent rapid spread of the virus led to its declaration as a global health emergency by the world health organization. the virus is transmitted mainly by the mosquito aedes aegypti, which is also the vector of dengue virus; however, little is known about the interactions of the virus with the mosquito vector. i ...201729202041
global risk mapping for major diseases transmitted by aedes aegypti and aedes albopictus.the objective of this study was to map the global risk of the major arboviral diseases transmitted by aedes aegypti and aedes albopictus by identifying areas where the diseases are reported, either through active transmission or travel-related outbreaks, as well as areas where the diseases are not currently reported but are nonetheless suitable for the vector.201729196275
larvicidal and residual activity of imidazolium salts against aedes aegypti (diptera: culicidae).aedes aegypti is an important species of mosquito, which can transmit several arboviruses such as dengue fever, yellow fever, chikungunya, and zika. as these mosquitoes are becoming resistant to most chemical insecticides used in the world, studies with new larvicides should be prioritized. based on the known biological profile of imidazolium salts (is), the objective of this study was to evaluate the potential of six is as larvicides against ae. aegypti, which was tested against ae. aegypti lar ...201729193680
first insights on insecticidal activity upon aedes aegypti and partial biochemical characterization of a novel low molecular mass chymotrypsin-trypsin inhibitor purified from lonchocarpus sericeus seeds.arboviroses such as dengue, zika and chikungunya represent a serious issue in public health due to the absence of approved vaccine or specific antiviral drugs. one way to prevent these diseases is by combating the vector mosquito, aedes aegypti (diptera), which has serine proteases in the midgut. protease inhibitors are molecules that can block enzyme activity impairing digestion and nutrition that can lead to death. thus, we purified and characterized a novel chymotrypsin- trypsin inhibitor fro ...201729193604
mapping the spatial distribution of aedes aegypti and aedes albopictus.mosquito-borne infectious diseases, such as rift valley fever, dengue, chikungunya and zika, have caused mass human death with the transnational expansion fueled by economic globalization. simulating the distribution of the disease vectors is of great importance in formulating public health planning and disease control strategies. in the present study, we simulated the global distribution of aedes aegypti and aedes albopictus at a 5×5km spatial resolution with high-dimensional multidisciplinary ...201829191515
insecticide susceptibility screening against culex and aedes (diptera: culicidae) mosquitoes from the united states.mosquitoes exposed to sublethal doses of insecticides may be selected for resistance to insecticide active ingredients (ais). mosquitoes are exposed to ais through agricultural, public/private mosquito control programs, homeowners, and other sources. hence, mosquito control programs should routinely measure the resistance/susceptibility status of mosquito populations of public health concern. the objectives here were to determine resistance status for six ais used in adult mosquito control in th ...201729186579
first evidence of zika virus venereal transmission in aedes aegypti mosquitoes.aedes aegypti is considered the main zika virus (zikv) vector, and is thought to be responsible for the 2015-2016 outbreak in brazil. zika positive ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of zikv may occur.201829185595
point-of-use nanobiosensor for detection of dengue virus ns1 antigen in adult aedes aegypti: a potential tool for improved dengue surveillance.dengue virus (denv) and its primary mosquito vectors aedes spp. have spread to every continent except antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. recently, the spread of other arboviruses carried by invasive aedes spp., such as chikungunya and zika, seem to be following similar trends as denv and have renewed interest in monitoring and modeling arboviruses within mosquito vectors. unfortunately, current commercially available detection methods ...201829182305
involvement of cecropin b in the formation of the aedes aegypti mosquito cuticle.in this study, we found a mosquito antimicrobial peptide (amp), aedes aegypti cecropin b (aacec b), was expressed constitutively in pupae. knockdown in the pupae of aacec b using double-stranded rna (dsrna) resulted in high mortality, the emergence of deformed adults and an impairment of pharate adult cuticle formation with fewer lamellae being deposited and the helicoidal pattern of the chitin microfibrils being disorganized. simultaneous injection of aacec b dsrna and aacec b peptide into pupa ...201729180688
a peptide signaling system that rapidly enforces paternity in the aedes aegypti mosquito.female aedes aegypti mosquitoes typically mate only once with one male in their lifetime, a behavior known as "monandry" [1]. this single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4- to 6-week lifespan in the laboratory [2]. successful mating induces lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male [3-5]. ae. aegypti mate in flight near human hosts [6], and females b ...201729174895
bacterial exopolysaccharide (eps)-coated zno nanoparticles showed high antibiofilm activity and larvicidal toxicity against malaria and zika virus vectors.in this study, a novel and effective approach was performed to synthesize zno nanoparticles (zno nps) using the exopolysaccharides (eps) from the probiotic strain bacillus licheniformis dahb1. eps acted as reducing and stabilizing agent for the formation of eps-zno nps by co-precipitation method. structural characterization was investigated by a surface plasma resonance centered at 375nm in uv-vis spectrum. ftir spectrum exhibited functional groups with strong absorption peak at 3814.7-420cm-1. ...201829173489
facile green synthesis of zinc oxide nanoparticles using ulva lactuca seaweed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity.the bioactivity of semiconductor nanocomplexes has been poorly studied in the field of pesticide science. in this research, the synthesis of zinc nanoparticles was accomplished through new effortless green chemistry process, using the ulva lactuca seaweed extract as a reducing and capping agent. the production of u. lactuca-fabricated zno nanoparticles (ul-zno nps) was characterized by powder x-ray diffraction (xrd), uv-visible, fourier transform infrared (ftir) spectroscopy, selected area elect ...201829169140
the importance of human population characteristics in modeling aedes aegypti distributions and assessing risk of mosquito-borne infectious diseases.the mosquito aedes aegypti has long been a vector for human illness in the southeastern united states. in the past, it has been responsible for outbreaks of dengue, chikungunya, and yellow fever and, very recently, the zika virus that has been introduced to the region. multiple studies have modeled the geographic distribution of ae. aegypti as a function of climate factors; however, this ignores the importance of humans to the anthropophilic biter. furthermore, ae. aegypti thrives in areas where ...201729167627
determination of the efficiency of diets for larval development in mass rearing aedes aegypti (diptera: culicidae).larval diet quality and rearing conditions have a direct and irreversible effect on adult traits. therefore, the current study was carried out to optimize the larval diet for mass rearing of aedes aegypti, for sterile insect technique (sit)-based applications in sri lanka. five batches of 750 first instar larvae (l 1) of ae. aegypti were exposed to five different concentrations (2-10%) of international atomic energy agency (iaea) recommended the larval diet. morphological development parameters ...201729166980
biology of two larval morphological phenotypes of aedes aegypti in abidjan, côte d'ivoire.since 2008, several outbreaks of yellow fever and dengue occurred in abidjan, the economic capital of côte d'ivoire. a better knowledge of the biology of aedes aegypti populations, the main vector of yellow fever and dengue viruses, is necessary to tailor vector control strategies implemented in the city. this study was designed to determine some biological parameters, occurring during the life cycle of two morphological phenotypes of ae. aegypti larvae. mosquitoes were sampled in a suburb of ab ...201729166962
feeding-induced changes in allatostatin-a and short neuropeptide f in the antennal lobes affect odor-mediated host seeking in the yellow fever mosquito, aedes aegypti.aedes aegypti is a model species in which the endogenous regulation of odor-mediated host seeking behavior has received some attention. sugar feeding and host seeking in female a. aegypti are transiently inhibited following a blood meal. this inhibition is partially mediated by short neuropeptide f (snpf). the paired antennal lobes (als), as the first processing centers for olfactory information, has been shown to play a significant role in the neuropeptidergic regulation of odor-mediated behavi ...201729166650
wolbachia infection alters the relative abundance of resident bacteria in adult aedes aegypti mosquitoes, but not larvae.insect-symbiont interactions are known to play key roles in host functions and fitness. the common insect endosymbiont wolbachia can reduce the ability of several human pathogens, including arboviruses and the malaria parasite, to replicate in insect hosts. wolbachia does not naturally infect aedes aegypti, the primary vector of dengue virus, but transinfected ae. aegypti have antidengue virus properties and are currently being trialled as a dengue biocontrol strategy. here, we assess the impact ...201729165845
insecticide susceptibility status in mexican populations of stegomyia aegypti (= aedes aegypti): a nationwide assessment.in mexico, mosquito vector-borne diseases are of public health concern as a result of their impact on human morbidity and mortality. the use of insecticides against adult mosquitoes is one of the most common ways of controlling mosquito population densities. however, the use of these compounds has resulted in the development of insecticide resistance. the aim of this study was to estimate susceptibility to six pyrethroids, two carbamates and two organophosphates in mexican populations of stegomy ...201729165810
larvicidal, histopathological efficacy of penicillium daleae against larvae of culex quinquefasciatus and aedes aegypti plus biotoxicity on artemia nauplii a non-target aquatic organism.mosquitoes can transmit the terrible diseases to human beings. soil-borne fungal products act as potential source for low-cost chemicals, used for developing eco-friendly control agents against mosquito-vector borne diseases. the prime aim of study was to check the larvicidal potential of fungus mycelia (by ethyl acetate solvent) extract from penicillium daleae (kx387370) against culex quinquefasciatus and aedes aegypti and to test the toxicity of brine shrimp artemia nauplii, by observing the p ...201729163159
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