Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
a decrease in cysteine levels causes the glutathione deficiency of aging in the mosquito.our previous results indicated that a glutathione (gsh) deficiency is a determinant of the aging process in many tissues and organisms. correction of this deficiency in the aging mosquito by feeding the cysteine (cys) precursor magnesium thiazolidine carboxylic acid (mgtc) suggested that the cause could be a lack of cys. adult mosquitoes (aedes aegypti) were fed either a control diet or a diet supplemented with mgtc and then were analyzed for their cys, cystine, gsh, and glutathione disulfide co ...19883340632
further characterization of refractoriness in aedes aegypti (l.) to infection by dirofilaria immitis (leidy).factors which control the expression of the refractory or susceptible condition to infection with dirofilaria immitis in the mosquito. aedes aegypti, were investigated using three protocols. (1) microfilariae and prelarvae were injected into the hemocoel of susceptible a. aegypti. some microfilariae and prelarvae developed to the l1 larval stage but they failed to complete development to the infective stage. (2) enema of microfilariae and prelarvae from infected susceptible and refractory donor ...19883366210
fmrfamide- and adipokinetic hormone-like immunoreactivity in the nervous system of the mosquito, aedes aegypti.as demonstrated with immunocytochemistry, specific cells and axons in the nervous system of female aedes aegypti contain antigens immunologically related to fmrfamide (phenylalanine-methionine-arginine-phenylalanine-amide) and locust adipokinetic hormone i (akh). in the supra-esophageal ganglion, including some medial neurosecretory cells, and in all ganglia of the ventral nerve cord, there are 100-120 cells immunoreactive to a fmrfamide antiserum. the same cells cross-react with a bovine pancre ...19883372750
pathogenesis of the hyphomycete tolypocladium cylindrosporum in the mosquito aedes aegypti. 19883373005
time and site of assembly of the peritrophic membrane of the mosquito aedes aegypti.we determined the time and site of secretion of the precursors of the peritrophic membrane (pm) in aedes aegypti and when the structure is assembled. the fine structure of the developing membrane of blood-feed females was described, and the pattern of secretion of injected tritiated glucosamine analyzed autoradiographically. immediately following blood feeding, ingested red cells rapidly become compressed, such that the surrounding plasma is extruded to the margin of the midgut contents. thereby ...19883383223
reexamination of chemically mediated oviposition behavior in aedes aegypti (l.) (diptera: culicidae). 19883392710
categorization of domestic breeding habitats of aedes aegypti (diptera: culicidae) in northern queensland, australia. 19883392713
susceptibility & transmissibility of different geographical strains of aedes aegypti mosquitoes to chikungunya virus. 19883397145
[the experimental pathogenic effect of spiroplasma isolated from mosquitoes on the hatching of aedes aegypti ova and the growth of larva hatched from these eggs].the authors did study the experimental effects on aedes aegypti ova of different spiroplasma strains, isolated from mosquitoes in french savoy and in taiwan. the sp7 strain, from armigeres subalbatus (taiwan), demonstrates a true pathogenic effect on the larval evolution, without sex ratio modifications, nor bacterial transmission to the adult mosquitoes. the authors present their results and emphasize the difficult use of spiroplasmas sp.19883400962
defense reactions by larvae of aedes aegypti during infection by the aquatic fungus lagenidium giganteum (oomycete).the adherence of zoospores of lagenidium giganteum to the cuticle of mosquito larvae is the initial step in the infection process. subsequently, a germ tube penetrates the integument, inducing a rapid melanization of the injured cuticle and epidermis. after entering the hemocoel the developing hyphae are occasionally encapsulated locally. this process is slow (6 to 12 h postincubation) and most frequently cell-free, although it can be mediated by circulating hemocytes. sporadic hemocyte mediatio ...19883416342
properties of a ribonuclease from aedes aegypti larvae.1. the properties of a soluble ribonuclease from aedes aegypti larvae have been compared with ribonuclease activity in adult female tissue. 2. in larval extracts ribonuclease activity was maximal at 40-45 degrees c whereas activity in tissue from adult females was highest at 50 degrees c. 3. ribonuclease activity that was recovered in a 20-60% ammonium sulfate precipitate was further purified by batch elution from deae-sephacel and from carboxymethylcellulose. 4. ribonuclease activity in the par ...19873427905
aedes aegypti and yellow fever virus: the effect of chloroquine on infection and transmission rates. 19873445296
[development and basic properties of the viral preparation viroden].laboratory studies demonstrated the effectiveness of viroden preparation in different doses against preimago stages of aedes aegypti mosquitoes. after treatment of larvae of instar 1 at preimago stages about 77% of the insects died. the preparation is stable on storage and resistant to unfavourable environmental factors: sun radiation, heating, ph variations, without significant loss of activity. the study of the host range of densonucleosis virus, the active principle of the viroden preparation ...20163445591
overproduction of detoxifying esterases in organophosphate-resistant culex mosquitoes and their presence in other insects.antisera raised against the denatured polypeptide of two organophosphate-detoxifying esterases (b1 and a1) of culex mosquitoes were used in an immunoblot method to quantify esterase production in resistant versus susceptible strains and to detect the presence of immunologically related proteins in other insects. it was demonstrated that esterase b1 of culex quinquefasciatus and esterase a1 of culex pipiens are overproduced in resistant strains by factors of at least 500-fold and 70-fold, respect ...19873470782
control of aedes aegypti larvae in household water containers by chinese cat fish.in 1980-81 an outbreak of dengue fever occurred in guangdong province and in guangxi-zhuang autonomous region in the central-southern part of china. subsequently, a nationwide survey indicated that the vector of the disease, aedes aegypti, was confined to the coastal strip of guangdong and guangxi-zhuang. since the first case in the outbreak occurred in guangxi-zhuang, a community-based programme to control a. aegypti was set up in eight fishing villages of this region where the mosquito was bre ...19873500803
increased yellow fever virus infection and dissemination rates in aedes aegypti mosquitoes orally exposed to freshly grown virus. 19873503398
a behavioral mechanism for resting site selection by pupae in three mosquito species.pupae of aedes aegypti, ae. triseriatus and culex restuans dive less frequently when resting in a concave meniscus than when resting in open water. they also tend to terminate diving after contacting submerged vertical surfaces, increasing their chances of surfacing in a concave meniscus. as a result pupae tend to rest in concave menisci associated with emergent vertical surfaces, a behavioral adaptation by which they probably conserve energy and avoid predation.19873504897
strategies for the emergency control of arboviral epidemics in new orleans.a study was initiated to evaluate the effectiveness of aerial ulv spraying for the control of aedes aegypti and culex quinquefasciatus as an emergency antiepidemic measure against dengue fever and st. louis encephalitis, respectively. malathion was aerially applied at 219 ml/ha over 344 ha area of the ninth ward in new orleans. laboratory susceptibility tests and field-cage exposures indicated that the target populations were susceptible to malathion and that acceptable coverage of the study are ...19873504901
an alternative bioassay employing neonate larvae for determining the toxicity of suspended particles to mosquitoes.an alternative bioassay using neonate larvae of aedes aegypti is described for determining the toxicity of suspended particles, particularly insecticidal bacteria, to mosquitoes. this new assay is comparable in precision to the 4th instar bioassay recommended by the world health organization, but is quicker and requires much less space. values obtained using neonate larvae as the test insect showed less variation within and between replicates in regard to lc50 and slope of the regression line, a ...19873504908
susceptibility of aedes aegypti and anopheles quadrimaculatus larvae to infection with the cercariae of plagiorchis noblei (trematoda: plagiorchiidae).the impact of interspecific behavioral differences on the relative susceptibility of third instar aedes aegypti and anopheles quadrimaculatus larvae to infection with cercariae of plagiorchis noblei was determined. when permitted to move freely in a column of water, larvae of ae. aegypti were significantly more susceptible to infection with the parasite than were an. quadrimaculatus larvae. this difference is ascribed to the significantly greater activity of ae. aegypti larvae in the water colum ...19873504909
variation in the vector competence of geographic strains of aedes albopictus for dengue 1 virus.eight geographic strains of aedes albopictus from asia and north america and one north american strain of aedes aegypti were tested for their vector competence with dengue 1 virus. three groups of ae. albopictus were established based on their vector competence: a) the oahu laboratory strain, b) the three malaysian strains, and c) the tokyo and three north american strains. the three north american strains were similar to the strain of ae. aegypti from houston, texas in their ability to transmit ...19873504922
the origin and movement of gas during adult emergence in aedes aegypti: an hypothesis.several events which precede adult emergence in aedes aegypti were studied, including changes in buoyancy, changes visible through the pupal cuticle and changes at the foregut-midgut junction. our data suggest tht the gas which is present in the posterior midgut at the time of emergence originates in the tracheal system. during the process of emergence this gas moves into the exuvial space through the adult spiracles and then follows the exuvial fluid into the alimentary canal.19873504927
factors affecting the acquisition of plagiorchis noblei (trematoda: plagiorchiidae) metacercariae by larvae and pupae of aedes aegypti in the laboratory.when exposed to concentrations of less than one plagiorchis noblei cercariae per cc of water, the acquisition of metacercariae by aedes aegypti larvae increased significantly with each successive instar but declined precipitously upon pupation. thus, 1st instar larvae acquired no metacercariae, whereas 4th instars had a mean abundance of 6.15: pupae only acquired a mean of 0.62 parasites. parasite acquisition was largely a function of host size and activity. other factors, such as grooming and f ...19873504947
the genetic basis of resistance and sensitivity to the meiotic drive gene d in the mosquito aedes aegypti l.a study has been made on the genetic basis of meiotic drive at the distorter (d) locus which, in coupling with the male-determining gene (or region) m on the y chromosome, causes production of excess male progeny. its effect is regulated by the sensitivity/resistance of the x chromosome. this study demonstrates that there are two major loci controlling resistance/sensitivity to md: (1) the m gene (or region) on the x chromosome (allelic with m) which may be either mr or ms (resistant or sensitiv ...19873505880
natural transmission of dirofilaria immitis by aedes aegypti.the liverpool strain of the mosquito aedes aegypti was infected with microfilariae of the canine heartworm, dirofilaria immitis, and was used to transmit heartworm larvae to three dogs. methods of confirming heartworm infection in these dogs included the modified knott's test, a commercial enzyme-linked-immunosorbent assay (elisa), an indirect fluorescent antibody (ifa) test, and post-mortem examination.19863507470
the size of emerging and host-seeking aedes aegypti and the relation of size to blood-feeding success in the field.the average wing length of aedes aegypti females collected as pupae was 2.47 mm, which was significantly smaller than the 2.64 mm average wing length of the host-seeking females collected in the field. the average wing length of nulliparous host-seeking females was 2.62 mm, which was significantly smaller than the 2.76 mm wing length of parous host-seeking females. thus, small ae. aegypti females exhibited reduced blood-feeding success and, most likely, reduced survival when compared with large ...19863507471
aedes aegypti larvae in portsmouth, rhode island. 19863507481
evaluation of the cdc gravid trap for the surveillance of st. louis encephalitis vectors in memphis, tennessee.collections with cdc gravid mosquito traps on 954 trap-nights from may through september 1983 are compared to 2,608 concurrent resting site collections made in the same area of memphis, tennessee. gravid traps yielded 88 times more culex per collection and 96 times more culex per man hour. the total gravid trap catch was 135,724 mosquitoes, 99% of which were either culex pipiens or cx. restuans, whereas these species comprised only 63% of the 6,613 mosquitoes collected from resting sites. gravid ...19863507491
a laboratory study of cyromazine on aedes aegypti and culex quinquefasciatus and its activity on selected predators of mosquito larvae.in a laboratory study, the insect growth regulator, cyromazine, exerted a high level of biological activity on aedes aegypti and culex quinquefasciatus treated in the 4th larval instar. at 1.5 and 1.0 ppm this igr produced 97 and 99% inhibition of emergence in adult ae. aegypti, respectively. in cx. quinquefasciatus, there was 99% inhibition at 1 ppm and complete inhibition at 1.5 ppm. the overall pupal mortality was higher than larval or adult stages of both species. this material induced diffe ...19863507502
effect of three pyrethroids on blood feeding and fecundity of aedes aegypti.caged aedes aegypti were exposed to a range of concentrations of d-phenothrin, d-allethrin and tetramethrin in a wind tunnel. mortality, blood engorgement and egg production among these mosquitoes and their progeny were recorded. tetramethrin was the most effective (lc50 of 0.0017%), followed by d-phenothrin (lc50 0.0031%) and d-allethrin (lc50 0.01%). blood engorgement was decreased by treatment with each pyrethroid at the high concentration (0.002%, 0.003% and 0.01% respectively). treatment wi ...19863507504
effects of sublethal exposure to bacillus thuringiensis var. israelensis on larval development and adult size in aedes aegypti.the effects of exposure to sublethal concentrations of bacillus thuringiensis var. israelensis (serotype h-14) on second instar aedes aegypti larvae were investigated. a test system was developed in which adverse effects would be detected as increased duration of larval development and decreased adult body size. no evidence of negative effects on survivors could be detected when sufficient b.t.i. dosages were applied to kill approximately half of the larvae in the treatment groups. however, when ...19863507506
toxicity of pyrethroids to aedes aegypti larvae in relation to temperature.the influence of temperature on the toxicity of the pyrethroids cypermethrin, permethrin, fenvalerate, d-phenothrin, flucythrinate and bioallethrin to 3rd instar aedes aegypti larvae was determined. based on lc50 levels, the toxicities of all pyrethroids were in the range of 1.33- to 3.63-fold greater at 20 degrees c than at 30 degrees c. our laboratory results suggest that for larval control of ae. aegypti, field performance of these pyrethroids may be reduced at warmer temperatures.19863507510
the perspective of aedes albopictus from the administrative viewpoint.mosquito control administrators should consider a newly introduced species in view of local conditions. aedes albopictus was discovered in harris county while surveying for aedes aegypti. recognizing a potential threat to public health, local and state officials were notified of the discovery. questions posed by the introduction of a foreign species requires a cooperative application of research resources. the centers for disease control (cdc) should serve as the coordinator of this effort.19863507521
laboratory and field studies with 32p labeled toxorhynchites rutilus rutilus.females and eggs of toxorhynchites r. rutilus were labeled with 32p by feeding fourth-stage larvae 32p labeled aedes aegypti larvae. eggs from females up to 3 weeks in age had detectable levels of radioactivity and individual eggs contained ca. 0.3% of the mother's total radioactivity. comparisons of labeled and unlabeled females in indoor and outdoor cage tests indicated that survival and fecundity of the 2 groups were approximately equal. no differences were noted for dispersal and fecundity o ...19863507524
a model for polygenic inheritance of abdominal tergal scale pattern in aedes aegypti.there is much variation in the amount of white scaling on the abdominal tergites of aedes aegypti. the genetic basis for this white scale pattern was investigated in two laboratory strains established by selection from the carn strain of ae. aegypti. these experimental strains were crossed in all possible directions in single pair matings. based on analysis of their progeny it is proposed that genes at three separate independently assorting loci control abdominal tergal scale pattern. correlatio ...19863507526
toxicity in carcasses of bacillus thuringiensis var. israelensis-killed aedes aegypti larvae against scavenging larvae: implications to bioassay. 19863507532
association of chlamydia trachomatis with mammalian and cultured insect cells lacking putative chlamydial receptors.the association of radiolabelled chlamydia trachomatis (strains dk-20 and 434) with cultured cell monolayers has been examined. previously, n-acetyl neuraminic acid and n-acetyl-glucosamine had been suggested to be involved in the association of certain strains of c. trachomatis with cultured cell monolayers. we have now studied the association of non-lgv (dk-20) and lgv (434) strains of c. trachomatis with cultured cells lacking these putative receptor components. comparison of the association ...19873507553
[the aedes aegypti problem in brazil]. 19873507740
the vitellogenin gene family of aedes aegypti.we have been interested in identifying genes that play a role in reproduction of the mosquito aedes aegypti. our interests are currently focused on the vitellogenin genes which in the mosquito are expressed only in the fat body in response to the insect steroid hormone, 20-hydroxyecdysone. four of the five vitellogenin genes in the genome have been cloned. we have examined the relationships between these genes and find that they form a small gene family exhibiting different levels of relationshi ...19873509110
complement effects on the infectivity of plasmodium gallinaceum to aedes aegypti mosquitoes. i. resistance of zygotes to the alternative pathway of complement.gametocytes are the intraerythrocytic stages of malaria parasites that infect mosquitoes. when gametocytes of the chicken malaria parasite plasmodium gallinaceum are ingested by a mosquito they become extracellular in the mosquito midgut, form gametes, and fertilize within 10 to 15 min after the insect has taken a blood meal. gametocytes of p. gallinaceum were infectious when fed to aedes aegypti mosquitoes in blood meals containing native serum from chickens or from the non-host species, man or ...19863517168
bacteriolytic factor in the salivary glands of aedes aegypti.salivary gland homogenates from adult aedes aegypti lyse micrococcus lysodeikticus cells. the bacteriolytic factor is present in a cell type common to both male and female mosquitoes, as well as in the crop of sugar-feeding mosquitoes. the bacteriolytic factor releases digestion products from sacculi of escherichia coli that are different from those of hen egg white lysozyme.19863519067
mosquito trypsin: immunocytochemical localization in the midgut of blood-fed aedes aegypti (l.).a polyclonal antibody was raised against trypsin purified from the midgut of blood-fed aedes aegypti. using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. the activity was localized mainly in the posterior part of the distensible, abdominal midgut, along the periphery of the blood bolus and within the peritrophic membrane. immunoreactivity appeared 8 h after th ...19863524850
aedes aegypti mosquitoes in the americas: a review of their interactions with the human population.this paper describes the significant changes in the relationship between aedes aegypti mosquitoes and the human population in the americas from the 1800s to present. first, the history of the arrival of the mosquito, the consequent epidemics of yellow fever and dengue, and the eventual implication of the mosquito as vector is described. second, the pan american health organization's aedes aegypti eradication program is discussed and the potential threat to the americas of development of the deng ...19863532349
expression in escherichia coli of a cloned crystal protein gene of bacillus thuringiensis subsp. israelensis.a ca. 10-kilobase (kb) hindiii fragment of plasmid dna from bacillus thuringiensis subsp. israelensis was cloned into plasmid puc9 and transformed into escherichia coli. extracts of the recombinant strain contained a 27-kilodalton (kda) peptide that reacted with antibodies to a 27-kda peptide isolated from crystals produced by b. thuringiensis subsp. israelensis. extracts of the recombinant strain were hemolytic and toxic to aedes aegypti larvae. full expression of the 27-kda peptide required th ...19873546262
ribosome metabolism during the vitellogenic cycle of the mosquito, aedes aegypti.ribosome accumulation and synthesis in the fat body of the mosquito aedes aegypti increased by approx. 4-fold during 18 h after the blood meal, consistent with the pattern of total rna accumulation during the synthetic phase of the vitellogenic cycle. by 24-30 h after the blood meal, the accumulated ribosomes began to be degraded, and the total rna content in the fat body eventually returned to previtellogenic levels. a method has been developed for isolation of ribosomal subunits from fat body, ...19873567223
[are sugars always necessary for the infection of mosquitoes with malarial plasmodia?].the possibility of the completion of the sporogony cycle of plasmodium gallinaceum in aedes aegypti mosquitoes, which were not given preliminary carbohydrate feeding, is considered. the significance of the obtained data for tracing the connection between the type of feeding of arthropods and their ability to be specific vectors of agents of transmissible infections is discussed.19873574981
the impact of sequential ultra-low volume ground aerosol applications of malathion on the population dynamics of aedes aegypti (l.).the efficacy of sequential, ultra-low volume ground aerosol applications of malathion at current u.s. label rates was evaluated as an emergency control measure for adult populations of aedes aegypti (l.) in new orleans, louisiana. replicates of 11 sequential aerosol treatments applied 12 hr apart during a 5.5-day period reduced mean adult captures and oviposition rates during the treatment period 73% and 75%, respectively. we hypothesize that oviposition was not completely suppressed because fem ...19873578659
multiple forms of cerebral peptides with steroidogenic functions in pupal and adult brains of the yellow fever mosquito, aedes aegypti.in the adult mosquito, aedes aegypti, the cerebral egg development neurosecretory hormone (ednh) regulates reproduction by activating the ovaries to synthesize the steroid hormone ecdysone, while during postembryonic development the cerebral prothoracicotropic hormone (ptth) activates the prothoracic glands to synthesize ecdysone. in each case, ecdysone than drives stage-specific physiological processes. since ednh and ptth share a common steroidogenic function, it is conceivable that they are s ...19873582726
inheritance of ddt dehydrochlorination and of a mechanism restricting uptake of ddt in the mosquito aedes aegypti.crosses and backcrosses were made between the t8 dichlorodiphenyltrichloroethane (ddt) resistant strain and ns susceptible strain. each generation was tested for resistance, for internal levels of ddt and dichlorodiphenyldichloroethylene (dde) (thus "ddt uptake" (ddt + dde) and percentage ddt dehydrochlorination in vivo), and for ddt dehydrochlorination in vitro, both at the larval and adult stages. the patterns of inheritance of uptake and dehydrochlorination were different. at both life stages ...19873582976
potential for organophosphate resistance in aedes aegypti (diptera: culicidae) in the caribbean area and neighboring countries. 19873585923
a study on susceptibility of indonesia colonies of aedes aegypti and aedes albopictus mosquitoes to experimental infection with dengue type 3 and chikungunya viruses. 19873586577
multiplication of chikungunya virus in salivary glands of aedes albopictus (oahu strain) mosquitoes: an electron microscopic study.aedes albopictus as well as aedes aegypti is an important vector of chikungunya and dengue viruses. electron microscopic observations on the salivary glands of ae. albopictus infected with chikungunya virus were performed in comparing with those of ae. aegypti infected with dengue virus. no virus budding from the cell surface of the chikungunya-infected mosquito's salivary glands was found as shown in dengue-infected ones, in contrast to the findings of the mammalian cells such as vero, kb, imr, ...19863599528
development of brugia malayi and dirofilaria immitis in aedes aegypti: effect of the host's nutrition.the purpose of the study was to establish the importance of single and multiple blood meals taken by a vector in the development of larval filariae, dwelling in tissues other than the fat body. the black eye strain of aedes aegypti was used as experimental vector for brugia malayi and dirofilaria immitis, filariae species which develop in the flight muscles and malphighian tubules respectively. under similar conditions, both filariae species molted for the first time on the 6th day and a second ...19873602836
increased dissemination of dengue 2 virus in aedes aegypti associated with concurrent ingestion of microfilariae of brugia malayi.we investigated whether concurrent ingestion of dengue 2 virus and microfilariae of brugia malayi would increase viral infection and dissemination rates in aedes aegypti. infection rates were similar in mosquitoes that ingested virus alone or both virus and microfilariae concurrently. however, viral dissemination rates, as determined by recovery of dengue virus from both legs and bodies separately, were significantly greater in mosquitoes that ingested both agents concurrently than in those that ...19873605503
purification of the mosquitocidal and cytolytic proteins of bacillus thuringiensis subsp. israelensis.two proteins from parasporal crystals of bacillus thuringiensis subsp. israelensis were purified to electrophoretic homogeneity by gel filtration and anion-exchange chromatography. the larger of the two proteins (molecular weight, 68,000) was not cytolytic, whereas the smaller protein (molecular weight, 28,000) was highly cytolytic when assayed against rat erythrocytes. when these proteins were assayed against larvae of the yellow fever mosquito, aedes aegypti, the larger protein was at least 10 ...19873606108
genetic studies on hexokinase in the mosquito aedes togoi.hexokinases (ec 2.7.1.1) were genetically analyzed in the mosquito aedes togoi by agar gel electrophoresis. enzyme activity was observed anodally in one major banding region (hk-1) on the gel and in another faintly stained region (hk-2). a total of six bands was detected in the hk-1 region. all six bands could be detected in three body parts, head, thorax, and abdomen, of adults with different banding intensities. the third and fourth bands, numbered from the more anodal side, showed the broades ...19873619882
life cycle and mode of infection of leptolegnia chapmanii (oomycetes) parasitizing aedes aegypti. 19873624894
linkage relationships between a low-mobility esterase locus and group i markers in larvae of the yellow fever mosquito, aedes aegypti (diptera: culicidae). 19873625727
ultrastructural midgut events in culicidae larvae fed with bacillus sphaericus 2297 spore/crystal complex.ingestion of bacillus sphaericus 2297 spore/crystal complex by culicidae larvae anopheles stephensi, culex pipiens subsp. pipiens and aedes aegypti was rapidly followed by a dissolution of the protein crystalline inclusions inside the anterior stomach of the three species. during the first day of intoxication, b. sphaericus spores germinated within the midgut lumen, and were in a vegetative stage between 36-48 h after ingestion when the larvae began to die. ultrastructural observations focused o ...19873663390
absence of transovarial transmission of chikungunya virus in aedes aegypti & ae. albopictus mosquitoes. 19873666861
the problem of aedes aegypti in brazil. 19863685524
seasonal incidence and diel patterns of oviposition in the field of the mosquito, aedes aegypti (l.) (diptera:culicidae) in trinidad, west indies: a preliminary study.diel patterns of oviposition of domestic aedes aegypti in the field in trinidad, west indies were monitored weekly for one year using standard ovitraps. during the wet season (may to november) a large, well defined peak of oviposition (comprising more than 80% of eggs laid) occurred one to two hours before sunset, and a much smaller peak (about 5% of eggs laid) occurred one to two hours after sunrise. during the dry season (december to may) a broad, poorly defined peak (about 36% of eggs laid) f ...19873689024
influence of developing dirofilaria immitis on the spontaneous flight activity of aedes aegypti (diptera: culicidae). 19873694634
selection for resistance to bacillus thuringiensis subspecies israelensis in field and laboratory populations of the mosquito aedes aegypti. 19863701104
the migration of infective larvae of brugia pahangi within the mosquito, aedes aegypti.the course of the migration of infective larvae of brugia pahangi within the mosquito, aedes aegypti, is described. the presence of blood or eggs in the abdomens of mosquitoes at a time when the infective larvae are beginning their migration out of the thorax leads to a significant increase in the proportion of larvae accumulating in the abdomen. the evidence supports the hypothesis that infective larvae may accumulate in the labium as a direct consequence of the insect's internal anatomy i.e. i ...19863714302
improved development of brugia microfilariae following cryopreservation in liquid nitrogen using a technique suitable for field conditions.a technique for improved cryopreservation at -196 degrees c of brugia spp. microfilariae has been developed by modifications of a procedure previously used with onchocerca spp. a double incubation in ethanediol (ed) solutions, firstly at 37 degrees c in 10% (v/v) ed for 15 min and secondly at 0 degrees c in 40% (v/v) ed for 45 sec followed by plunging into liquid nitrogen, resulted in over 90% of the microfilariae of b. malayi exhibiting normal motility. when used with b. pahangi microfilariae, ...20133726977
defense reactions of mosquitoes to filarial worms: effect of host age on the immune response to dirofilaria immitis microfilariae.the melanization response of aedes aegypti black-eyed liverpool strain (lvp) and aedes trivittatus against intrathoracically inoculated dirofilaria immitis microfilariae (mff) was assessed in mosquitoes less than 1, 14, 21, and 28 days after adult ecdysis. there was a significant decrease in the melanization response of a. aegypti 14 days of age and older at 1, 3, and 5 days postinoculation (pi) compared to less than 1-day-old mosquitoes. the response also was reduced significantly in 14- to 28- ...19863734989
parasite-induced suppression of the immune response in aedes aegypti by brugia pahangi.the melanization response against intrathoracically inoculated brugia pahangi and dirofilaria immitis microfilariae (mff) isolated from vertebrate host blood was evaluated in both uninfected aedes aegypti black-eyed liverpool strain and in mosquitoes harboring a developing b. pahangi infection. the immune response against inoculated mff of either species was significantly reduced by 28-47% in infected as compared with uninfected mosquitoes. attempts to passively transfer this suppression factor( ...19863734990
hemocyte-mediated melanization of microfilariae in aedes aegypti.the melanization response of adult female aedes aegypti (black-eyed liverpool strain) against intrathoracically inoculated dirofilaria immitis microfilariae (mff) was assessed with transmission electron microscopy. the initial reaction involved the lysis of hemocytes at or near the surface of the parasite prior to the deposition of pigment. subsequently, melanin formation was noted in the area of lysed cells and appeared to cascade onto the parasite surface. observations suggest that melanin may ...19863734991
ultrastructural organization of spermatocysts in the testes of aedes aegypti (diptera: culicidae). 19863735341
influence of developing brugia pahangi on spontaneous flight activity of aedes aegypti (diptera: culicidae). 19863735352
fmrfamide- and pancreatic polypeptide-like immunoreactivity of endocrine cells in the midgut of a mosquito.immunocytochemical surveys of midguts from female mosquitoes, aedes aegypti, reveal that half of the estimated 500 endocrine cells in a midgut contain a substance recognized by antisera to bovine pancreatic polypeptide and a molluscan peptide, fmrfamide (phenylalanine-methionine-arginine-phenylalanine-amide). with light microscopy the cells resemble an endocrine type because of their basal position in the epithelium, conical shape, and, in some instances, apical extensions to the lumen. at the u ...19863738889
changes in ribonuclease activity during development of the mosquito, aedes aegypti.in the mosquito aedes aegypti, quantitative and qualitative changes have been detected in ribonuclease activity during development. ribonuclease activity is particularly high in extracts from larvae, relative to that in extracts from pupae or adults. larval extract is enriched for a ribonuclease that is heat-labile, has an alkaline ph optimum, and is extremely sensitive to the divalent cation, manganese. extract from adult females is enriched for a heat-stable component that has an acidic ph opt ...19863743028
trichostrongylus colubriformis: isolation and characterization of ovicidal activity from bacillus thuringiensis israelensis.bioassay of media fractions from cultivation of bacillus thuringiensis israelensis revealed that ovicidal activity for eggs of the ruminant nematode trichostrongylus colubriformis was found in microbial crystals, but was not released into culture medium. the purified delta-endotoxin of b. t. israelensis, composed of two 25 kda proteins, had no effect on nematode eggs. a fraction that had high ovicidal activity for eggs of t. colubriformis was isolated by high performance liquid chromatography fr ...19863743716
the ability of aedes aegypti mosquitoes to survive and transmit infective larvae of brugia pahangi over successive blood meals.the mortality of aedes aegypti mosquitoes increased; immediately following a blood meal containing microfilariae of brugia pahangi, when infective larvae began to migrate out of the flight muscles and when infective larvae were lost from the mosquitoes during a blood meal. when infective mosquitoes took a second blood meal 86.2% of the infective larvae escaped from their bodies. however, only 50.3% escaped when mosquitoes fed through a thin layer of cotton. infective larvae in the abdomen of the ...19863745870
the effects of larval density, temperature and ph on the culture growth and infectivity of saprolegnian sp. zoospores to aedes aegypti larvae at various instars in the laboratory. 19863759180
etiologic and serologic investigations of the 1980 epidemic of dengue fever on hainan island, china.virologic and seroepidemiologic studies were carried out during an epidemic of dengue fever on hainan island in 1980. dengue 3 virus was isolated from 46 of 77 acute phase sera and from 1 of 10 pools of adult aedes aegypti. dengue 1 virus virus was isolated from a single acute phase serum. seroepidemiologic investigations showed that 74% of healthy individuals in the epidemic area had antibody to dengue virus compared to 54% in an area where epidemic dengue had occurred in 1978, and less than or ...19863766851
application of environmental management principles in the program for eradication of aedes (stegomyia) aegypti (linneus, 1762) in the republic of cuba, 1984. 20103768599
comparative studies on the melanization response of male and female mosquitoes against microfilariae.the melanization response of adult male and female aedes trivittatus and the black-eyed liverpool strain of aedes aegypti against intrathoracically inoculated dirofilaria immitis microfilariae (mff) was assessed at 1, 3, and 5 days postinoculation (pi). the melanization reaction of males is significantly less effective than the response elicited by female mosquitoes. no mff in male a. aegypti and only 17% of mff recovered from a. trivittatus were fully melanized by day 5 pi compared with 80% and ...19863770266
analysis of mosquito larvicidal potential exhibited by vegetative cells of bacillus thuringiensis subsp. israelensis.vegetative bacillus thuringiensis subsp. israelensis cells (6 x 10(5)/ml) achieved 100% mortality of aedes aegypti larvae within 24 h. this larvicidal potential was localized within the cells; the cell-free supernatants did not kill mosquito larvae. however, they did contain a heat-labile hemolysin which was immunologically distinct from the general cytolytic (hemolytic) factor released during solubilization of b. thuringiensis subsp. israelensis crystals. the larvicidal potential of the vegetat ...19863777922
ultrastructure of malpighian tubules of aedes aegypti infected with dirofilaria immitis. 19863782853
the effect of repeated blood meals on the larval development of dirofilaria corynodes in the fat body of aedes aegypti.the objective of the study was to determine whether blood meals taken by a vector subsequent to the infective one have any influence on the course of development of a filarial parasite in its tissue. the filaria dirofilaria corynodes (von linstow, 1899) was selected for study and the black-eye strain of aedes aegypti served as the experimental vector. the microfilaria of d. corynodes develops to the infective stage in the fat bodies of the vector. following an infective blood meal, mosquitoes we ...19863787121
dispersal and other population parameters of aedes aegypti in an african village and their possible significance in epidemiology of vector-borne diseases.dispersal of aedes aegypti aegypti adults within shauri moyo, an african village in the rabai area north of mombasa, kenya, was studied using the mark-release-recapture method. a total of 920 mosquitoes were captured and uniquely marked, of which 828 (90%) were released and 332 (40%) recaptured. a great majority of mosquitoes were recaptured once, but some individuals were recaptured up to 10 times. most females visited 1 or 2 houses (40.8%, 44.9%, respectively), but there were females that visi ...19863789275
passage of yellow fever virus: its effect on infection and transmission rates in aedes aegypti.the effect of successive lytic passage of yellow fever virus on mosquito infection and transmission rates in the vector, aedes aegypti, was determined. three strains of yellow fever virus from trinidad and peru were passaged five times in suckling mouse brains and seven times in bhk-21 cells. mosquitoes were fed meals containing passaged and unpassaged viruses and infection and transmission rates were compared. rates were similar for all but one of the three virus strains grown in both substrate ...19863789277
what can be learnt about selection from gene frequency distribution?polymorphism has been studied at the esterase 6 locus in the yellow fever mosquito aedes aegypti (l.) in laboratory stocks. at least 12 alleles are present, with up to four coexisting in a stock. the allele frequency distribution is quite sharply peaked at a mode of about 0.25. the experimental data are compared with the results of simulation based on two models, one in which the initial global distribution is taken to be the stationary distribution obtained from the neutral model assuming m = 4 ...03792826
surface changes on brugia pahangi microfilariae and their association with immune evasion in aedes aegypti. 19873794383
[use of surviving infectious larvae of dipetalonema dessetae in study and research on filaricidal substances].dipetalonema dessetae in proechimys oris, the natural final host is a rodent filariasis model used as an in vivo antifilarial screening test. the laboratory vector is aedes aegypti. infective larvae l3 isolated from the intermediate host develop and remain healthy for up to 30 days in a biphasic culture medium composed of a cell feeder layer (l 929) and rpmi 1640 supplemented with foetal calf serum. this culture technique has enabled us to screen antifilarial compounds on a new in vitro test. th ...20063795974
the 1982 emergency ultralow volume spray campaign against aedes aegypti adults in paramaribo, suriname. 19863801746
[new and unusual locations of aedes (stegomyia) aegypti, linné 1762 (diptera, culicidae) in madagascar].the author situates and comments three new aedes aegypti's locations in madagascar; one of which is in the international tananarive's airport's control area.19863802312
the effects of plagiorchis noblei metacercariae on the development and survival of aedes aegypti larvae in the laboratory.plagiorchis noblei infections impair the survival and development of fourth instar aedes aegypti larvae. mortality during the larval and pupal stages reached 92%, and 60% of the emerging adults were malformed. the metacercariae interfere with pupation and the emergence of adults. larvae and pupae that fail to transform to the next developmental stage within the normal time characteristically persist for extended periods, but invariably die without transforming. whereas 82% of the control larvae ...19863806320
microfilarial perforation of the midgut of a mosquito.to determine whether the midgut envelope of mosquitoes is disrupted by the passage of microfilariae, ultrastructural changes induced by microfilariae of brugia malayi were observed in midguts of aedes aegypti mosquitoes. basal and apical plasma membranes were destroyed, disrupting the full depth of the midgut wall. ingested ferritin lay against the gut wall, suggesting absence of the peritrophic membrane during penetration. exsheathment of microfilariae appears to be enhanced by movement against ...19863806321
effect of temperature on the vector efficiency of aedes aegypti for dengue 2 virus.the effect of temperature on the ability of aedes aegypti to transmit dengue (den) 2 virus to rhesus monkeys was assessed as a possible explanation for the seasonal variation in the incidence of dengue hemorrhagic fever in bangkok, thailand. in two laboratory experiments, a bangkok strain of ae. aegypti was allowed to feed upon viremic monkeys infected with den-2 virus. blood-engorged mosquitoes were separated into two groups and retained at constant temperatures. virus infection and transmissio ...19873812879
sclerotization of mosquito cuticle.the mode of sclerotization of aedes aegypti pupal and adult cuticle was examined by employing biochemical and radioactive techniques. during larval-pupal metamorphosis, tyrosine is converted to tanning precursors and is incorporated into aryl-amino adducts and beta-crosslinks. the major hydrolysis product of beta-crosslinks in pupal cases is identified to be arterenone. examination of tanning modes in five different mosquito species shows that the ratio of quinone to beta-sclerotization not only ...20133817100
hemocyte cell surface changes in aedes aegypti in response to microfilariae of dirofilaria immitis.this study involved the assessment of surface changes on hemocytes of aedes aegypti black-eyed liverpool strain in association with the melanization response against intrathoracically inoculated dirofilaria immitis microfilariae (mff). surface changes on hemocytes were identified using fluorescein-labeled wheat germ agglutinin (wga). in mosquitoes eliciting a melanization response against inoculated mff, there was a 5-fold increase in the percentages of hemocytes exhibiting wga binding compared ...19863819964
effect of plasmodium gallinaceum infection on the mortality and body weight of aedes aegypti (diptera: culicidae). 19873820241
avermectins, mk-933 and mk-936, for mosquito control.two avermectins were shown to be effective as mosquito larvicides or when fed to adult female mosquitoes in sucrose solution or in blood. larval lc50 values of compounds mk-933 and mk-936, expressed as parts per billion, were found to be 3.94 and 2.42 for culex pipiens, 5.85 and 2.90 for anopheles stephensi and 23.4 and 10.4 for aedes aegypti. when fed to adult females of an. stephensi, ae. aegypti or cx quinquefasciatus, mk-933 at 2.8mg active ingredient per litre in sucrose solution caused com ...19853832491
the susceptibility of aedes aegypti (l) larvae to a pathogenic non sexual saprolegnia sp. 19853833712
oral infection of aedes aegypti with yellow fever virus: geographic variation and genetic considerations.twenty-eight populations representing a worldwide distribution of aedes aegypti were tested for their ability to become orally infected with yellow fever virus (yfv). populations had been analyzed for genetic variations at 11 isozyme loci and assigned to one of 8 genetic geographic groups of ae. aegypti. infection rates suggest that populations showing isozyme genetic relatedness also demonstrate similarity to oral infection rates with yfv. the findings support the hypothesis that genetic variat ...19853834804
selection for susceptibility and refractoriness of aedes aegypti to oral infection with yellow fever virus.artificial selection on strains of aedes aegypti showing susceptibility and refractoriness to oral infection with yellow fever virus (yfv) suggests that there is a significant genetic component to this trait. using a population with an average susceptibility of 15%, inbreeding of isofemale lines followed by individual selection produced susceptible (29% infected) and refractory (11% infected) lines. the difference between lines was largely apparent before individual selection, which failed to in ...19853834805
rainfall, abundance of aedes aegypti and dengue infection in selangor, malaysia.an epidemio-meteorotropic analytical study of selangor, in the southwest coast of peninsular malaysia, examines the monthly incidence of dengue for the period 1973-1982 to assess possible quantitative association with the monthly rainfall. the relationships between rainfall, abundance of a. aegypti and dengue infection during 1982 in jinjang, a dengue-prone area in selangor, were also examined. a quantitative association between rainfall and the number of dengue cases was found during the first ...19853835698
Displaying items 701 - 800 of 13756