Publications

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measurement of aedes aegypti populations. 19676042077
toxicity of alkanes and alkenes to the pre-imaginal stages of the yellow-fever mosquito. 19676045138
reproductive behavior of aedes aegypti (l.) sublethally exposed to ddt. 19676052124
the effect of some anions and cations upon the fluxes and net uptake of chloride in the larva of aëdes aegypti (l.), and the nature of the uptake mechanisms for sodium and chloride. 19676058980
feeding and oviposition behavior in the mosquito aedes aegypti (l.). i. preliminary studies of physiological control mechanisms. 19676062263
aedes aegypti eradication program of the united states--progress report 1965. 19676066906
oviposition of aedes aegypti l. on a dry surface and hygroreceptors. 19676067781
status of aedes aegypti in the eastern mediterranean. 19676074036
[electrophysiological investigation of single olfactory receptors on the atennae of female mosquitoes (aedes aegypti l.)]. 19676077610
teratogenic effects of a tranquilizer, trifluoperazine dihydrochloride on the yellow-fever mosquito. 19676081038
resistance in aedes aegypti to chemosterilants: effect of apholate selection on resistance to apholate, tepa, and metepa. 19676081039
the fine structure of the spermathecae and their ducts in the mosquito aedes aegypti. 19676081062
[the ecology of dengue and aedes aegypti. preliminary investigation 4]. 19846084309
salivary apyrase of aedes aegypti: characterization and secretory fate.salivary gland homogenates of female adult aedes aegypti hydrolyzed atp and adp thereby defining an apyrase activity. activity is divalent cation dependent with an optimum ph of 9.0. atpase and adpase activities could not be dissociated thus suggesting the presence of a true apyrase enzyme. apyrase activity is low on the day of emergence but increases to 160 mu per pair of glands on the second day. the site at which mosquitoes probed into warm polyacrylamide gels retains apyrase activity, confir ...19846094095
chemically-defined media for production of insect cells and viruses in vitro.two chemically-defined media are described. they support the growth of a) an established cell line of spodoptera frugiperda cells and b) two established mosquito cell lines from aedes aegypti and anopheles gambiae. the replication of autographa californica nuclear polyhedrosis virus (acnpv) in s. frugiperda cells grown in a defined medium is reported.19806102531
dengue hemorrhagic fever in malaysia: the 1973 epidemic.the first major malaysian epidemic of dengue hemorrhagic fever with severe manifestations occurred in 1973, with 969 reported cases and 54 deaths. in a detailed study of 138 clinically diagnosed and laboratory confirmed cases at the general hospital in kuala lumpur, hemorrhagic manifestations were observed in 68.7% and shock in 18.1% of the patients. the cases occurred mainly from may to september, largely in urban and suburban areas of the majority of the states in the country. a main focus of ...19806105712
laboratory observations on the predation of toxorhynchites rutilus rutilus on aedes aegypti (diptera: culicidae). 19806106719
potential vectors of malaria and dengue at townsville, queensland.a biting collection on the banks of ross river included anopheles farauti, adedes imprimens and ae. alboscutellatus, indicating a relict population of rain-forest mosquitoes and confirming two earlier records of an. farauti from townsville. aedes aegypti was collected at townsville airport. significance of these findings is discussed in relation to changing urban conditions and a proposed international airport.19806111741
[transovarial transmission of arboviruses by mosquitoes (author's transl)].an important aspect of the epidemiology of arboviruses is the manner in which the viruses are maintained during winter, dry season, or other adverse environmental periods when their arthropod hosts are inactive. one possibility is that the viruses survive in arthropods. in the case of mosquito-borne viruses, it is probable that such viruses could be maintained in this manner only if they were transmitted from one insect generation to the next by transovarial transmission. such transmission was r ...19816116146
[arbovirus diseases in south america and caribbean islands (author's transl)].historically, yellow fever has dominated arboviral diseases in south america and the caribbean and sylvatic cases still occur every year (more than 200 in 1979). the dengue, by the very large number of cases occurring in the area during successive epidemics--the most important being the last one in 1977-1978--is a serious problem for public health, an epidemic of hemorrhagic dengue being a constant threat. encephalitides are the third syndrome of arboviral etiology observed in the south american ...19816116149
[arboviral diseases in south-west pacific islands (author's transl)].islands of the south-west pacific area belong to the melanesian group, excepted niue, tonga, wallis and futuna which are polynesian. through new guinea, there is a geographic relation to the eastern part of australia, rich of 42 arbovirus types. dengue and ross river fever are the most important arboviral diseases in the region; both affect islanders after introduction of virus by travellers to localities where efficient vectors are present. dengue types 1, 2 and 4 were isolated from man and fro ...19816116150
the effect of 5-fluorouracil and 5-fluorocytosine on the development of the filarial nematodes brugia pahangi and dirofilaria immitis.5-fluorouracil and 5-fluorodeoxyuridine at 30 mg/kg body weight daily for four days inhibit microfilarial production in brugia pahangi in the jird. disruption of intrauterine embryogenesis was observed in treated female worms but the compounds were not macrofilaricidal or microfilaricidal under the conditions employed. 5-fluorocytosine possessed no filaricidal or embryostatic activity. the inhibition of microfilaria production by 5-fluorouracil was temporary and larval production was resumed wit ...19816118037
role of the parasporal body in causing toxicity of bacillus thuringiensis toward aedes aegypti larvae. 19826120198
[study of the larvicidal activity of bacillus thuringiensis var. israelensis on toxorhynchitinae larvae (author's transl)].toxicity of bacillus thuringiensis var. israelensis, h 14 serotype, has been tested on larvae of toxorhynchites amboinensis, predaceous larvae of other culicinae larvae. the toxorhynchites larvae are not sensitive to the bacterial toxin up to relatively high dosages, except when larvae of aedes aegypti are provided in the suspension. mode of action of the crystal appears to be similar to the one observed in the epithelial cells of intoxicated aedes aegypti larvae midgut.19826125271
ingestion and development of wuchereria bancrofti in culex quinquefasciatus, anopheles gambiae and aedes aegypti after feeding on humans with varying densities of microfilariae in tanzania. 19826126022
comparative bioassays of bacillus thuringiensis h-14 formulations against four species of mosquitoes in malaysia.comparative laboratory bioassays of three formulations of bacillus thuringiensis h-14 (ips-78, san 402-i and bactimos) were conducted against late 3rd/early 4th instar larvae of four species of mosquito, viz., aedes aegypti, culex quinquefasciatus, anopheles balabacensis and mansonia (mansonioides) indiana, in malaysia. from the average response of the mosquito larvae to the three formulations of b. thuringiensis h-14, ae. aegypti was found to be most susceptible, followed by cx. quinquefasciatu ...19826128794
the effects of parabiotic twinning of susceptible and refractory mosquitoes on the development of plasmodium gallinaceum.two species of mosquitoes were joined parabiotically with glass capillaries so as to share common hemolymph. in experiments designed to determine optimum physical factors was found that capillaries of 2.5 mm in length, 100 microns od and with pointed ends were tolerated best by mosquitoes and permitted optimum hemolymph transfer. maximum survival of mosquitoes was noted when capillaries were inserted in the post mesospiracular membranous area, in the largest mosquito first and allowed to fill wi ...19826129293
diuresis in newly emerged, unfed mosquitoes. i. fluid loss in normal females and males during the first 20 hours of adult life.unfed mosquitoes (aedes aegypti) decapitated soon after emergence from the pupa survive better than those decapitated later. in a test involving more than 700 females and more than 600 males, 80% of the mosquitoes decapitated during the first hour of adult life survived a period of 45 h post emergence (p.e.), whereas about 80% of those decapitated at 12 h or later failed to survive the same period. there was a linear fall in the rate of survival between 1 and 10 h p.e., after which the rate fluc ...19826129627
effect of toxorhynchites brevipalpis on aedes aegypti (diptera: culicidae) in continuous-breeding laboratory populations. 19826130156
diuresis in newly emerged, unfed mosquitoes. ii. the basic pattern in relation to escape from the water, preparation for mature flight, mating and the first blood meal.a study of 2000 newly emerged adult mosquitoes (aedes aegypti) shows that the rate of excretion is high at the time of eclosion but falls rapidly over the next few minutes. in both sexes, however, the high rate of excretion is regained during a second peak in the middle of day 1. it remains high for about 6 h and then falls in a remarkably steady manner to reach a low level plain, which persists at least till the end of day 3. in females this plain is not reached till some 8 h after the onset in ...19836132392
cultivation of mosquito cell lines in serum-free media and their effects on dengue virus replication.seven mosquito cell lines from five species (aedes aegypti, ae. albopictus, ae. pseudoscutellaris, culex tarsalis, and toxorhynchites amboinensis) were adapted to three kinds of serum-free media (sem), which were composed of equal volumes of tryptose phosphate broth and of either leibovitz (l15) medium, eagle's minimum essential medium, or medium 199 with hanks' salts. population growth rates of the cells cultivated in the smfs were generally slower than those of original cell cultures maintaine ...19836137452
insecticidal evaluation of zolone against culex quinque-fasciatus, anopheles culicifacies, a. stephensi and aedes aegypti. 19836137454
the biological control of aedes aegypti on sa-med island, rayong province by means of toxorhynchites splendens, a predatory mosquito larva. 19836138386
experimental model of transovarial transmission of tahyna virus in aedes aegypti mosquitoes.the progeny of 31 viruliferous aedes aegypti females infected with tahyna virus by sucking on viraemic newborn mice was investigated for virus presence. out of 1587 individuals of the f1 generation, 16 suspensions representing the progeny of 7 females were positive in 146 trials. individuals of the f1 generation failed to transfer the virus by sucking. electron microscopy revealed the presence of tahyna virus particles in the cytoplasm of maturing oocytes inevitably confirming the transovarial t ...19836138986
the importance of disease induced changes in mammalian body temperature to mosquito blood feeding.laboratory mice infected with rodent malaria (plasmodium berghei or p. chabaudi) or st. louis encephalitis virus (sle) were not hyperthermic during the infection period. however, all infected animals displayed pathogen-specific periods of hypothermia. hamsters infected with p. berghei were hyperthermic on day 7 postinfection (pi) but became hypothermic on day 8 pi and remained so until death, approximately 20 days pi. body temperatures of mice infected with p. yoelii were not significantly diffe ...19846142803
the effect of p-aminobenzoic acid and folic acid on the development of infective larvae of brugia malayi in aedes aegypti.adult aedes aegypti mosquitoes, infected with the subperiodic brugia malayi, were found to enhance the development of the filarial parasites to the infective stage when they were exposed to a cotton pad soaked in 10% sucrose solution containing p-aminobenzoic acid (paba) in 0.001, 0.005, 0.01, 0.05 and 0.1% concentrations. similarly, larval development increased when the mosquitoes were fed with folic acid at 0.001, 0.01 and 0.1% concentrations. this stimulation was more when paba or folic acid ...19846143484
medium for the production of primary powder of bacillus thuringiensis subsp. israelensis.five media, formulated from the seeds of five legume varieties, dried cow blood, and mineral salts, were assessed for the growth and production of insecticidal properties of bacillus thuringiensis subsp. israelensis. bacterial powders prepared from the broth cultures were assayed against the larvae of aedes aegypti, culex quinquefasciatus, and anopheles gambiae. a standard primary powder of b. thuringiensis subsp. israelensis (ips78) was included in the assay for comparison. good growth was obta ...19846144290
effects of toxorhynchites amboinensis on laboratory-reared aedes aegypti populations (diptera: culicidae). 19846144797
[preliminary report on the susceptibility of mosquitoes to brugia pahangi].a comparative study on the susceptibility of eight species of four genera of mosquitoes to brugia pahangi was carried out. aedes aegypti (liverpool strain), aedes togoi, armigeres subalbatus and mansonia uniformis (taiwan strains) were found with the third-stage larvae 14 days after feeding on infected rats (sprague-dawley). the remaining of aedes aegypti, aedes albopictus, aedes pseudoalbopictus, culex p. fatigans and culex annutus (taiwan strains) were found refractory to infection. to determi ...19846150813
comparative bioassays of tolypocladium cylindrosporum gams (californian strain) against four species of mosquitoes in malaysia.comparative laboratory bioassays of tolypocladium cylindrosporum, california strain (kal) was conducted against third instar larvae of four species of mosquito, viz. aedes aegypti, anopheles balabacensis, culex quinquefasciatus and mansonia uniformis in malaysia. of the four mosquito species tested, ma. uniformis was found to be the most susceptible, followed by cx. quinquefasciatus, an. balabacensis and ae. aegypti, in a decreasing order. the lc50 values for ma. uniformis, cx. quinquefasciatus, ...19846151744
laboratory observations on toxorhynchites splendens wiedemann in thailand.the biology of toxorhynchites splendens predaceous larvae which breed in domestic containers like aedes aegypti was studied. egg stage lasts 52.64 +/- 0.13 hours. there are four larval instars lasting 2.38 +/- 0.04, 1.24 +/- 0.26, 8.09 +/- 1.6 and 28.78 +/- 5.39 days respectively. pupal development lasts 5.18 +/- 1.06 days. the adult life span is 29.55 +/- 8.06 days. growth increment was by measuring the size of head capsule. number of second instar prey larvae consumed by 1 to 4 instars predace ...19846151745
[research on viral infections in south pacific mosquitoes under natural conditions].from 1975, epidemiological studies on arboviral diseases, conducted in new caledonia, vanuatu, wallis and horne islands, have shown that 11 species of mosquitoes may harbour viruses in natural conditions. virus isolations were attempted from 1,025 pools of culicidae totalizing 32,182 individuals belonging to 5 genera and 21 species. viral infections have been demonstrated in 76 pools (7.4%). the species harbouring viruses were: aedes aegypti, ae. alternans, ae. notoscriptus, ae. vexans, ae. vigi ...19846151879
the effect of cytosine arabinoside on the synthesis of rapidly labeled rna during dna replicating and non-dna replicating periods of the cell cycle.the effects of various concentrations of cytosine arabinoside (ara-c) on the rates of dna and rna synthesis were investigated during the peak of dna synthesis, using a naturally synchronized culture of aedes aegypti (mosquito) cells. during this stage of the cell cycle, the synthesis of both dna and rna was found to be progressively inhibited with increasing concentrations of ara-c. when the same concentrations of ara-c were added to the culture at a time period when no dna was being synthesized ...19806156009
aedes aegypti in a texas coastal county as an index of dengue fever receptivity and control.since galveston county, texas, is one of the high risk areas for the reintroduction of dengue, we have maintained an aedes aegypti surveillance program since 1977 by using ovitraps to determine the density and distribution of this species in 17 communities. a. aegypti adults were present primarily from may thrugh november, with higher positive ovitrap rates associated with periods of low rainfall. larvae collected from six different communities, when compared with a standard, insecticide-suscept ...19806160776
eradication of aedes aegypti on cayman brac and little cayman, west indies, with abate (temephos) in 1970-1971. 19826176286
the exchange of calcium in larvae of the mosquito aedes aegypti.the exchange of labelled calcium between the external medium and the whole body was investigated in the larva of aedes aegypti (l.) using a closed, two-compartmental model. the transport system for the uptake of ca2+ was found to be saturable and obeyed michaelis-menten kinetics. the efficiency of the inward transport of calcium from dilute solutions was markedly reduced by starvation or by ruthenium-red, a selective inhibitor of ca2+ activated atpase, indicating that this transport system is en ...19836192197
evaluation of a controlled-release silicate formulation of temephos against aedes aegypti larvae in the laboratory and psorophora columbiae larvae (diptera: culicidae) in rice field plots. 19836192242
toxicity of cyclic peptide antibiotics to larvae of aedes aegypti. 19836196417
the toxic effects of triorganotin(iv) compounds on the culicine mosquito, aedes aegypti (l).the structure-activity relationships of 25 triorganotin(iv) compounds were studied using the 4th instar a. aegypti larvae of the susceptible liverpool red-eye strain. the lc50 values of these compounds were compared with those of malathion, ddt and temephos. maximal activities were obtained with tritolyltin chloride and its complexes with triphenyl-phosphine oxide and pyridine n-oxide, dimethyloctyltin- and diethyloctyltin acetates and tributyltin sucrose phthalate. a limited number of the trior ...19846204424
[the initial activity and persistance of an experimental larvicide with a base of bacillus thuringiensis var. israelensis and a commercial preparation containing the organophosphorus insecticide temephos].the larvicide activity of the experimental preparation san 402 i wdc containing b. thuringiensis var. israelensis and that of abate 500 e (44 % temephos) were tested on the larvae of aedes aegypti. the studies were performed on the following media: distilled water, pure and buffered at ph 9, 7 and 4 and pond water whether or not free from materials in suspension. the activity of both preparations is not influenced by the composition of the media, exception made for the presence of materials in s ...19846208235
the mosquito aedes aegypti (l.): evidence for three new proteinases.the effect of inhibitors was studied on the proteolytic activity of crude extracts of the mosquito aedes aegypti (l.), prepared 25 h after blood intake. this activity is only partially inhibited by the lima bean trypsin inhibitor (lbti) or by edta. experiments with mixed inhibitors are used to indicate that, apart from the well characterized trypsin-like enzymes, at least three other proteinases are present. these are an lbti-resistant serine proteinase, a metal chelator-sensitive proteinase, an ...19806245543
transovarial transmission of murray valley encephalitis virus by aedes aegypti (l).in laboratory studies, murray valley encephalitis virus was transmitted transovarially by orally infected aedes aegypti to approximately 1.5% of both adult male and female progeny.19806263238
zika virus, a cause of fever in central java, indonesia.in 1977 and 1978 selected in-patients at the tegalyoso hospital, klaten, indonesia who had recent onsets of acute fever were serologically studied for evidence for alphavirus and flavivirus infections. a brief clinical history was taken and a check list of signs and symptoms was completed on admission. acute and convalescent phase sera from 30 patients who showed evidence that a flavivirus had caused their illnesses were tested for neutralizing antibodies to several flaviviruses which occur in s ...19816275577
the measurement of blood meal size in aedes aegypti (l.).three techniques of estimating blood meal volumes (gravimetric, radioisotope counting and haemoglobin estimation) were compared in adult female aedes aegypti fed on a cat. aedes aegypti ingested a mean volume of 2.37 ul, 2.44 ul and 2.57 ul as measured by gravimetric, radioisotope and haemoglobin estimation techniques respectively. this difference was not statistically significant (p greater than 0.05, n = 58). neither calendar age (post-eclosion) nor strain differences appear to influence blood ...19806282090
[reproduction of powassan and west nile viruses in aedes aegypti mosquitoes and their cell culture]. 19826289063
specificity of cultured insect tissue cells for bioassay of entomocidal protein from bacillus thuringiensis.cultured tissue cells from lepidopteran and dipteran sources displayed an order-specific response to entomocidal protein from crystals of bacillus thuringiensis. protein isolated from crystals of b. thuringiensis subsp. kurstaki was effective against cells of the spruce budworm (choristoneura fumiferana) and the tobacco hornworm (manduca sexta), but was inactive against both mosquito cell lines tested (aedes aegypti and anopheles gambiae). conversely, protein from inclusion bodies of b. thuringi ...19846321329
peritrophic membranes and protease activity in the midgut of the malaria mosquito, anopheles stephensi (liston) (insecta: diptera) under normal and experimental conditions.formation and solubility of the peritrophic membranes (pm) in the midgut (stomach) of female anopheles stephensi depended on salt concentration in the gut lumen. high calcium drastically reduced the solubility of the pm in vitro. thin-layer chromatography revealed the presence of n-acetylgalactosamine and galactose in the pm. in contrast to aedes aegypti no n-acetylglucosamine was present indicating significant differences between anopheles and aedes mosquitoes with respect to pm formation and c ...19836345801
previtellogenic development and vitellogenin synthesis in the fat body of a mosquito: an ultrastructural and immunocytochemical study.we describe two phases, previtellogenic and vitellogenic, in the activity of the trophocytes in the fat body of the mosquito aedes aegypti. the previtellogenic phase, leading to trophocyte competence to synthesize vitellogenin (vg), occurred during the first 3 days after eclosion. this phase was characterized by enlargement and activation of the nucleoli, proliferation of ribosomes and rough endoplasmic reticulum (rer), development of golgi complexes, and extensive invaginations of the plasma me ...19836349013
a survey of filariasis among refugees in south florida.between january 1981 and march 1982, a filariasis survey was conducted among 668 haitian immigrants and 155 southeast asian refugees residing in florida, u.s.a. microfilariae were detected only in haitians, with 6.7% positive for wuchereria bancrofti and 1.3% positive for mansonella ozzardi. the majority of individuals with bancroftian filariasis came from five coastal urban areas including port-au-prince, duvalierville, gonaïves, leógane and cap-haitien. no unequivocal signs of bancroftian fila ...19846370004
[ecology of dengue and aedes aegypti. preliminary study]. 19846372125
selective primary health care: strategies for control of disease in the developing world. xi. dengue.since world war ii, dengue viruses have progressively extended their geographic domain and have increased as causes of human morbidity and mortality. this complex of four flaviviruses is principally transmitted to humans by the bite of aedes aegypti, the yellow fever vector. factors that promote the indoor storage of water are congenial to the breeding of a. aegypti. these include the dislocations of wars, overpopulation , and urbanization. by the mid-20th century, a. aegypti eradication campaig ...19846374837
arthropod studies with rabies-related mokola virus.a cell culture-adapted variant of the rabies-related mokola virus was demonstrated to replicate in inoculated aedes aegypti mosquitoes. replication was slow compared to many arboviruses in their vectors. maximum titers were not obtained until after approximately 6 weeks of extrinsic incubation. mokola virus underwent nine mosquito-mosquito passages at approximately monthly intervals and was thus maintained in insects for 340 days before terminating the study. virus antigen was detected by immuno ...19846385743
the use of dragonfly nymphs in the control of aedes aegypti.the predatory rates of the dragonfly nymphs on aedes aegypti were studied in the laboratory and under field conditons. labellulid nymphs were found to predate on ae. aegypti larvae and pupae readily. the rate of consumption was found to be 133 +/- 21 all stages of larvae per medium size nymph per 24 hours. in container habitats complete elimination of all larvae and pupae were achieved between day 4 and 9 depending on density of aquatic stages. the dragonfly nymphs as predators could be used in ...19806447358
[comparative action of solubilized crystals of h-14 and h-1 serotypes of bacillus thuringiensis on cultures of aedes aegypti cells].crystals isolated from bacillus thuringiensis serovar thuringiensis (h-1) and from serovar israelensis (h-14) were dissolved in sodium hydroxide, then tested in vitro on aedes aegypti cell cultures. toxic action was followed after morphological and metabolic alterations of the treated cells. only the israelensis sodium extract was toxic and gave cellular swelling, inhibition of cell growth and inhibition of labelled precursor incorporation, i. e. of 3h-thymidine, 3h-uridine and 3h-leucine.19846465742
[the ecology of dengue and aedes aegypti. preliminary report. 3]. 19846474264
the effect of colonization upon aedes aegypti susceptibility to oral infection with yellow fever virus.two colonies of aedes aegypti were established from two independent collections from vero beach, florida. eleven sequential generations of the first colony were tested for variation in oral susceptibility to infection with yellow fever virus (yfv). each generation was also assayed for genetic variability at seven enzyme loci using electrophoretic techniques. significant differences in infection rates were detected between some generations. these differences were significantly correlated with gen ...19846476217
lithium attenuates the active transport of calcium in the larva of aëdes aegypti. 19846491591
quantitative structure-activity relationship of insect juvenile hormone mimetic compounds.juvenile hormone mimetic activities on aedes aegypti (yellow-fever mosquito) and tenebrio molitor (yellow mealworm) of compounds having (2e,4e)-3,7,11-trimethyl-2,4-dodecadienone structures were comparatively and quantitatively analyzed in terms of their physiochemical structural parameters and by regression analysis. they were structurally composed of three classes, ester and thiol ester derivatives, amides, and ketones, depending on the c1 substituents. the results indicated that the steric di ...19846492079
esterase activity in strains of aedes aegypti (diptera: culicidae) tolerant and susceptible to the organophosphate insecticide malathion. 19846492084
genetic variation in an urban population of aedes aegypti (diptera: culicidae). 19846502629
paradoxical effects of simulated larviciding on production of adult mosquitoes.we determined whether simulated mortality of larval mosquitoes in food-deprived bodies of water increases emergence of adult females as well as their size by releasing the remaining larvae from competition for food. removal of larval aedes aegypti from rearing containers with suboptimal amounts of food increased the number of adults that emerged. thus, larvicidal measures do not inevitably lead to reduced numbers of mosquito vectors of disease. in food-limiting breeding sites, partially effectiv ...19846507734
the inactivation of acetylcholinesterase by alpha-terthienyl and ultraviolet light. studies in vitro and in larvae of the mosquito aedes aegypti.acetylcholinesterase (ec 3.1.1.7) was inactivated photochemically in solution, in the presence of dissolved terthiophene sensitizers. alpha-terthienyl (2,2':5,2"-terthiophene) and its isomers 3,2':5',2"- and 3,2':5',3"-terthiophenes showed very similar sensitizing properties. with all three terthiophenes, the photosensitization was completely suppressed under anaerobic conditions, and therefore the inactivation process required the presence of oxygen. the enzyme was inactivated in vivo when four ...19846509086
the verification of a mammalian toxicant classification using a mosquito screening method.routine toxicity screening of substances using rodents can be time consuming and expensive. for these reasons various alternative methods have been developed, but many lack standardization and acceptance. it was hypothesized that a satisfactory classification of mammalian toxicants could be obtained using a simple mosquito (aedes aegypti) screening method. to this end, median lethal concentration (lc50) values were determined for a wide variety of mammalian toxicants representing a range of toxi ...19846519371
[fecundity of aedes aegypti mosquitoes in relation to their infection with the malaria agent plasmodium gallinaceum].studies of the effect of p. gallinaceum on ae. aegypti in different periods of the agent's sporogony and during different gonotrophic cycles of the vector have shown the absence of strong inhibition of mosquitoes' fecundity.19846522100
comparative development of brugia malayi in susceptible and refractory genotypes of aedes aegypti. 19846527177
amino acids and peptides. xi. synthesis of attractant and repellent peptides for aedes aegypti and blattella germanica. 19846532560
infectivity of a venezuelan strain of metarhizium anisopliae to aedes aegypti larvae. 19846539354
hormonal regulation of ovarian ecdysteroid production in the autogenous mosquito, aedes atropalpus.the effects of juvenile hormone (jh) and egg development neurosecretory hormone (ednh) on ovarian ecdysteroid production during vitellogenesis in the autogenous mosquito, aedes atropalpus, were investigated using in vitro techniques coupled with radioimmunoassay (ria) and high-pressure liquid chromatography (hplc). normal females were characterized by quantitative, qualitative, and temporal patterns of in vitro ovarian ecdysteroid production. females decapitated at emergence showed little ovaria ...19846541607
natural transovarial transmission of dengue 4 virus in aedes aegypti in trinidad.the isolation of dengue 4 virus from adult aedes aegypti, reared from eggs collected in nature, is reported for the first time. from the locality where the isolate was made, 25 pools consisting of 1,848 ae. aegypti reared from eggs were processed. in this study, 10 different localities were sampled and a total of 10,957 ae. aegypti adults, collected as eggs or larvae in nature, were processed for virus isolation. from a total of 158 mosquito pools tested, one recovery of dengue 4 virus was made. ...19846542323
[laboratory study of the transmission of dengue virus by aedes aegypti and aedes albopictus]. 19846543740
the regulation of preimaginal populations of aedes aegypti (l.) (diptera: culicidae) on the kenya coast. ii. food as a main regulatory factor.experiments were carried out in a village on the kenya coast to investigate observed differences in pupal productivity of aedes aegypti breeding in indoor pots. daily addition of maize gruel, the staple food of villagers, to pots of high and low mosquito production promoted an increase of pupal numbers, although daily introduction of first-instar larvae did not significantly affect pupal production. differences in pupal productivity were not related to differences in the numbers of eggs laid in ...19846547039
development of dirofilaria repens in aedes aegypti reared in contrasting habitat.the susceptibility of dirofilaria repens infection in aedes aegypti adults reared in hay-infusion and tap-water during their larval stages was tested. ae. aegypti reared in hay-infusion ingested a bigger amount of blood than those reared in tap-water but harboured fewer d. repens larvae (developing and arrested). the difference in the susceptibility of mosquitoes from the two treatments was correlated with the difference in the robustness of the mosquitoes.19836612416
breeding of aedes aegypti in closed septic tanks. 19836618537
transovarial transmission of dengue viruses by mosquitoes: aedes albopictus and aedes aegypti.transovarial transmission of all four dengue serotypes was demonstrated in aedes albopictus mosquitoes. the rates of such transmission varied with the serotype and strain of virus. in general, the highest rates were observed with strains of dengue type 1 and the lowest with dengue type 3. surprisingly, despite the use of viral strains of the four dengue serotypes which gave the highest rates with ae. albopictus, transovarial transmission was observed in aedes aegypti only with dengue type 1, and ...19836625066
toxicity of bacillus thuringiensis subsp. israelensis to adult aedes aegypti mosquitoes.adult female aedes aegypti mosquitoes were killed by the parasporal crystals of bacillus thuringiensis subsp. israelensis (onr-60a) when the crystals were introduced into the insect midgut as an enema. the 50% lethal dose for intact parasporal crystals was 0.21 microgram/mg of mosquito (wet weight), and for solubilized crystals the 50% lethal dose was 0.04 microgram/mg. these values were compared with 50% lethal concentrations in a free-feeding larval mosquito bioassay of 0.018 and 1.28 microgra ...19836625566
the problems of aedes aegypti control in the americas. 19836626807
[diseases transmitted by mosquitoes and urbanization. examples of urban vectors of dengue and filariasis].once located in time and place the beginning of urbanization process, the mechanisms of adaptation to urban ecosystem and of dissemination by human conveyances are studied for two domestic mosquitoes: aedes aegypti and culex pipiens fatigans (= quinque-fasciatus). then epidemiological consequences are discussed. ae. aegypti pullulation constitutes a potential risk of urban yellow fever outbreak and the main factor of dengue haemorrhagic fever appearance; the increase of c. p. fatigans population ...19836627544
[new regioselective synthesis of racemic 7,11-dimethyloctadecane--an ovipositon attractant for the yellow fever mosquito (aedes aegypti)]. 19836628194
monoclonal antibodies against surface determinants on gametes of plasmodium gallinaceum block transmission of malaria parasites to mosquitoes.monoclonal antibodies (mab) against gametes of the chicken malaria plasmodium gallinaceum have been derived. all reacted with the surface of extracellular gametes of the parasite in immunofluorescent antibody reactions and all agglutinated both male and female gametes. in the absence of active complement one mu isotype mab, la 1-d5, mediated at least 95% suppression of infectivity of the parasites to aedes aegypti mosquitoes. individually, mab of the gamma 1 or gamma 2a isotypes mediated only sl ...19836631012
relative toxicity of organic solvents to aedes aegypti larvae. 19836631047
[action of the delta-endotoxin of bacillus thuringiensis var. israelensis on cultured cells from aedes aegypti l].the effects of bacillus thuringiensis var. israelensis delta-endotoxin were investigated on a cell culture of aedes aegypti with the electron microscope. the ultrastructural changes following intoxication were: disintegration of endoplasmic reticula by the formation of spherical structures; condensation and then swelling of mitochondria with disappearance of internal cristae; frequent dilatation of the perinuclear space; cellular lysis. the action of a metabolic inhibitor, 2,6-dibromophenol (2,6 ...19836638755
vertebrate host specificity and experimental vectors of plasmodium (novyella) kempi sp. n. from the eastern wild turkey in iowa.vertebrate host specificity, experimental laboratory vectors, and a description of plasmodium (novyella) kempi sp. n. from eastern wild turkeys (meleagris gallopavo silvestris vieillot) in iowa are presented. plasmodium kempi is infective for domestic turkeys, bobwhites (colinus virginianus), chukars (alectoris graeca), guinea fowl (numida meleagris), peacocks (pavo cristatus), and canaries (serinus canaria), produces a transient infection in mallards (anas platyrhynchos) and domestic geese (ans ...19836644918
transmission of dengue virus by orally infected aedes triseriatus.transmission of dengue type 1 was demonstrated for 3 strains of aedes triseriatus mosquitoes after oral infection. rates of infection were similar to those observed in a control strain of aedes aegypti. three additional species belonging to the subgenus protomacleaya (aedes brelandi, aedes hendersoni, and aedes zoosophus) were also susceptible to oral infection with dengue type 1 virus but transmission could not be demonstrated although virus was detected in the salivary glands of infected mosqu ...19836650743
a case study of aedes aegypti prevalence by settlement types in dehu town group of maharashtra state. 19836668009
linkage relationships of eleven enzyme loci in the aedes scutellaris group.linkage relationships of 11 enzyme loci were determined in backcrosses between aedes polynesiensis and aedes kesseli. three linkage groups established were aat2-lap2-me-sex, cat-ao-pgm-idh2-est6, and gpi-odh-pgd. lap2 and cat have not been previously mapped in aedes. locus order and linkage groups were the same as those observed for seven loci mapped in aedes aegypti. the significance of the observed similarities in chromosome organization and differences in crossover values among closely relate ...19836670992
susceptibility of aedes aegypti to infections with dirofilaria immitis and dirofilaria repens.eight stocks of aedes aegypti-4 geographic stocks and 4 selected stocks-were tested for their susceptibility to infection with dirofilaria immitis and d. repens. four of the stocks were found to be susceptible and the other 4 were refractory of infection with d. immitis. all the stocks of ae. aegypti that were susceptible to d. immitis were also susceptible to d. repens but the stocks that were refractory to d. immitis were all partially susceptible to d. repens.19836673132
insecticidal effect of aliphatic alcohols against aquatic stages of aedes mosquitoes.effectiveness of certain long carbon chain aliphatic alcohols as ovicides and larvicides was investigated under laboratory conditions against eggs and larvae of aedes aegypti and aedes scutellaris. out of the eight alcohols tested, three (1-decanol, 1-undecanol and 1-dodecanol) proved to be very effective. the hatching of mosquito eggs was inhibited at a dosage of 5 to 7 litres/hectare. first, third and fourth instar larvae were killed at a dosage of 3 to 7 litres/hectare. first instar larvae we ...19836683011
characterization of antigens on mosquito midgut stages of plasmodium gallinaceum. i. zygote surface antigens.we have defined the surface protein antigens on plasmodium gallinaceum zygotes using radioiodination methods and rabbit anti-zygote serum which blocks transmission of the parasite to aedes aegypti mosquitoes. fifteen protein bands (1-15) in the molecular weight range of 40 000-240 000 and one band at the bromophenol blue dye marker were labelled by the lactoperoxidase and iodogen (1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril) methods. the localization of these radioiodinated components ...19836683783
increased intradermal probing time in sporozoite-infected mosquitoes.because malaria sporozoites destroy segments of the salivary glands of vector mosquitoes, we determined whether salivary function is impaired. such pathology would result in a prolonged intradermal probing phase of feeding behavior, because the role of saliva is to help locate blood vessels. indeed, non-infected aedes aegypti mosquitoes probed for a shorter period than did either sporozoite-infected or saliva-deprived mosquitoes. salivary apyrase activity is reduced to a third following maturati ...19846696175
dengue fever. reappearance in northern queensland after 26 years.during march, 1981, a number of cases of dengue fever occurred in cairns and townsville, northern queensland. from october, 1981, an outbreak of the infection was recognized on thursday island and, by may 1982, an estimated 38% of residents had been infected. isolated cases were reported from other towns in northern queensland and from other islands in the torres strait. clinical presentation varied from that of severe incapacitating illness lasting up to seven days to infections which were conf ...19846700459
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