Publications

TitleAbstractYear
Filter
PMID
Filter
sexual and vertical transmission of zika virus in anti-interferon receptor-treated rag1-deficient mice.although zika virus (zikv) is primarily transmitted to humans by the aedes aegypti mosquito, human-to-human transmission has also been observed from males-to-females as well as mother-to-offspring. in the current study, we studied both sexual transmission (stx) and vertical transmission (vtx) of zikv using anti-ifnar1-treatment of rag1 (-/-) (air) mice. these mice have suppressed type i ifn responses and lack adaptive immune responses, leading to a prolonged infection prior to clinical disease. ...201728775298
culex quinquefasciatus from areas with the highest incidence of microcephaly associated with zika virus infections in the northeast region of brazil are refractory to the virus.zika virus (zikv) is widely distributed in brazil and the northeast region (ne) is the most affected zone, showing the highest incidence of microcephaly associated with zikv congenital infections worldwide. we report attempts to infect three populations of culex quinquefasciatus from severely affected sites in the ne and southeast region (se) of brazil with three strains of zikv isolated from these localities. an aedes aegypti population from the se was used as a positive control. all tested cx. ...201728767975
linking only aedes aegypti with zika virus has world-wide public health implications. 201728736548
zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process.zika disease has transformed into a serious global health problem due to the rapid spread of the arbovirus and alarming severity including congenital complications, microcephaly and guillain-barré syndrome. zika virus (zikv) is primarily transmitted to humans through the bite of an infective mosquito, with aedes aegypti being the main vector.201728728607
asymptomatic transmission and the dynamics of zika infection.following the 2013-14 outbreak in french polynesia, the zika virus (zikv) epidemic spread widely to many countries where aedes aegypti as the main transmitting vector is endemic. the lack of a second wave of zikv infection in most affected regions may suggest that a sufficiently high level of herd immunity was reached during the first wave. we developed an agent-based transmission model to investigate the role of asymptomatic infection on the likelihood of observing a second wave, while accounti ...201728724972
american aedes vexans mosquitoes are competent vectors of zika virus.abstractstarting in 2013-2014, the americas have experienced a massive outbreak of zika virus (zikv) which has now reached at least 49 countries. although most cases have occurred in south america and the caribbean, imported and autochthonous cases have occurred in the united states. aedes aegypti and aedes albopictus mosquitoes are known vectors of zikv. little is known about the potential for temperate aedes mosquitoes to transmit zikv. aedes vexans has a worldwide distribution, is highly abun ...201728719283
early evidence for zika virus circulation among aedes aegypti mosquitoes, rio de janeiro, brazil.during 2014-2016, we conducted mosquito-based zika virus surveillance in rio de janeiro, brazil. results suggest that zika virus was probably introduced into the area during may-november 2013 via multiple in-country sources. furthermore, our results strengthen the hypothesis that zika virus in the americas originated in brazil during october 2012-may 2013.201728628464
a single substitution changes zika virus infectivity in mosquitoes.zika virus (zikv) has caused outbreaks in the pacific and the americas. the mechanism underlying the recent zikv epidemic remains obscure. a recent study reveals that an amino acid substitution is associated with increased infectivity of zikv in the aedes aegypti mosquito.201728610876
zika vector transmission risk in temperate australia: a vector competence study.zika virus is an emerging pathogen of global importance. it has been responsible for recent outbreaks in the americas and in the pacific region. this study assessed five different mosquito species from the temperate climatic zone in australia and included aedes albopictus as a potentially invasive species.201728599659
emerging infectious disease agents and blood safety in australia: spotlight on zika virus.emerging infectious diseases (eids) are infectious diseases whose incidence has increased in humans in the past 20 years or could increase in the near future. eid agents may represent a threat to blood safety if they infect humans, cause a clinically significant illness, include an asymptomatic blood phase in the course of infection, and are transmissible by transfusion. eid agents are typically not well characterised, but there is a consensus that we can expect ongoing outbreaks. strategies to ...201728566073
genomic epidemiology reveals multiple introductions of zika virus into the united states.zika virus (zikv) is causing an unprecedented epidemic linked to severe congenital abnormalities. in july 2016, mosquito-borne zikv transmission was reported in the continental united states; since then, hundreds of locally acquired infections have been reported in florida. to gain insights into the timing, source, and likely route(s) of zikv introduction, we tracked the virus from its first detection in florida by sequencing zikv genomes from infected patients and aedes aegypti mosquitoes. we s ...201728538723
de novo generation and characterization of new zika virus isolate using sequence data from a microcephaly case.zika virus (zikv) has recently emerged and is the etiological agent of congenital zika syndrome (czs), a spectrum of congenital abnormalities arising from neural tissue infections in utero. herein, we describe the de novo generation of a new zikv isolate, zikvnatal, using a modified circular polymerase extension reaction protocol and sequence data obtained from a zikv-infected fetus with microcephaly. zikvnatal thus has no laboratory passage history and is unequivocally associated with czs. zikv ...201728529976
outbreak of zika virus infection in singapore: an epidemiological, entomological, virological, and clinical analysis.an outbreak of zika virus infection was detected in singapore in august, 2016. we report the first comprehensive analysis of a national response to an outbreak of zika virus infection in asia.201728527892
eco-friendly and cost-effective ag nanocrystals fabricated using the leaf extract of habenaria plantaginea: toxicity on six mosquito vectors and four non-target species.recently, the biofabrication of metal nanoparticles has gained wide interest owing to its inherent features such as swift, simplicity, eco-friendliness, and cheaper costs. different green-reducing agents led to the production of nanoparticles with varying toxicity on insects. in the current study, silver nanoparticles (agnps) were successfully synthesized using habenaria plantaginea leaf extract. ag nanoparticles were studied by uv-vis spectroscopy (uv-vis), x-ray diffraction (xrd), fourier tran ...201728523613
evolutionary enhancement of zika virus infectivity in aedes aegypti mosquitoes.zika virus (zikv) remained obscure until the recent explosive outbreaks in french polynesia (2013-2014) and south america (2015-2016). phylogenetic studies have shown that zikv has evolved into african and asian lineages. the asian lineage of zikv was responsible for the recent epidemics in the americas. however, the underlying mechanisms through which zikv rapidly and explosively spread from asia to the americas are unclear. non-structural protein 1 (ns1) facilitates flavivirus acquisition by m ...201728514450
a location-specific spreadsheet for estimating zika risk and timing for zika vector surveillance, using us military facilities as an example.local zika virus transmission in the united states involving one or both of the known vector species, aedes aegypti and ae. albopictus, is of major concern. to assist efforts to anticipate the risks of transmission, we developed an excel spreadsheet tool that uses vector and virus temperature thresholds, remotely sensed maximum temperature, and habitat suitability models to answer the questions: "is zika transmission likely here?" and "when should we conduct vector surveillance?" an example spre ...201728511272
transmission incompetence of culex quinquefasciatus and culex pipiens pipiens from north america for zika virus.abstractin late 2014, zika virus (zikv; flaviviridae, flavivirus) emerged as a significant arboviral disease threat in the western hemisphere. aedes aegypti and aedes albopictus have been considered the principal vectors of zikv in the new world due to viral isolation frequency and vector competence assessments. limited reports of culex transmission potential have highlighted the need for additional vector competence assessments of north american culex species. accordingly, north american culex ...201728500817
potential of a northern population of aedes vexans (diptera: culicidae) to transmit zika virus.zika virus is an emerging arbovirus of humans in the western hemisphere. with its potential spread into new geographical areas, it is important to define the vector competence of native mosquito species. we tested the vector competency of aedes vexans (meigen) from the lake agassiz plain of northwestern minnesota and northeastern north dakota. aedes aegypti (l.) was used as a positive control for comparison. mosquitoes were fed blood containing zika virus and 2 wk later were tested for viral inf ...201728499036
a zika virus from america is more efficiently transmitted than an asian virus by aedes aegypti mosquitoes from asia.zika is a mosquito-borne disease associated with neurological disorders that causes an on-going pandemic. the first outbreak was recorded in micronesia in 2007, then in french polynesia in 2014 from which it spread to south america in 2015 and ignited a widespread epidemic. interestingly, zika outbreaks in asia remained of moderate intensity although the virus is circulating. to understand these epidemiological variations, we investigated the entomological determinants of zikv transmission in as ...201728450714
vector competence and transovarial transmission of two aedes aegypti strains to zika virus.zika virus (zikv) has become a serious threat to global health since the outbreak in brazil in 2015. additional chinese cases have continuously been reported since the first case of laboratory-confirmed zikv infection in china on 6 february 2016. aedes aegypti is the most important vector for zikv. this study shows that two strains from china exhibit high levels of midgut infection and highly disseminated infection of salivary glands and ovaries. both strains can transmit zikv to infant mice bit ...201728442754
effects of zika virus strain and aedes mosquito species on vector competence.in the western hemisphere, zika virus is thought to be transmitted primarily by aedes aegypti mosquitoes. to determine the extent to which ae. albopictus mosquitoes from the united states are capable of transmitting zika virus and the influence of virus dose, virus strain, and mosquito species on vector competence, we evaluated multiple doses of representative zika virus strains in ae. aegypti and ae. albopictus mosquitoes. virus preparation (fresh vs. frozen) significantly affected virus infect ...201728430564
competence of aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes as zika virus vectors, china.in china, the prevention and control of zika virus disease has been a public health threat since the first imported case was reported in february 2016. to determine the vector competence of potential vector mosquito species, we experimentally infected aedes aegypti, ae. albopictus, and culex quinquefasciatus mosquitoes and determined infection rates, dissemination rates, and transmission rates. we found the highest vector competence for the imported zika virus in ae. aegypti mosquitoes, some sus ...201728430562
evidence of zika virus rna fragments in aedes albopictus (diptera: culicidae) field-collected eggs from camaçari, bahia, brazil.a major mosquito-borne viral disease outbreak caused by zika virus (zikv) occurred in bahia, brazil, in 2015, largely due to transmission by the mosquito, aedes aegypti (l.). detecting zikv in field samples of ae. aegypti has proven problematic in some locations, suggesting other mosquito species might be contributing to the spread of zikv. in this study, several (five) adult aedes albopictus (skuse) mosquitoes that emerged from a 2015 field collection of eggs from camaçari, bahia, brazil, were ...201728419254
potential risk of re-emergence of urban transmission of yellow fever virus in brazil facilitated by competent aedes populations.yellow fever virus (yfv) causing a deadly viral disease is transmitted by the bite of infected mosquitoes. in brazil, yfv is restricted to a forest cycle maintained between non-human primates and forest-canopy mosquitoes, where humans can be tangentially infected. since late 2016, a growing number of human cases have been reported in southeastern brazil at the gates of the most populated areas of south america, the atlantic coast, with rio de janeiro state hosting nearly 16 million people. we sh ...201728687779
taking innovative vector control interventions in urban latin america to scale: lessons learnt from multi-country implementation research.prior to the current public health emergency following the emergence of chikungunya and zika virus disease in the americas during 2014 and 2015, multi-country research investigated between 2011 and 2013 the efficacy of novel aedes aegypti intervention packages through cluster randomised controlled trials in four latin-american cities: fortaleza (brazil); girardot (colombia), acapulco (mexico) and salto (uruguay). results from the trials led to a scaling up effort of the interventions at city lev ...201728829235
modeling the transmission and control of zika in brazil.zika virus, a reemerging mosquito-borne flavivirus, started spread across central and southern america and more recently to north america. the most serious impacted country is brazil. based on the transmission mechanism of the virus and assessment of the limited data on the reported suspected cases, we establish a dynamical model which allows us to estimate the basic reproduction number r 0 = 2.5020. the wild spreading of the virus make it a great challenge to public health to control and preven ...201728798323
species specific rna a-to-i editing of mosquito rdl modulates gaba potency and influences agonistic, potentiating and antagonistic actions of ivermectin.the insect gaba receptor, rdl, is the target of several classes of pesticides. the peptide sequences of rdl are generally highly conserved between diverse insects. however, rna a-to-i editing can effectively alter amino acid residues of rdl in a species specific manner, which can affect the potency of gaba and possibly insecticides. we report here that rna a-to-i editing alters the gene products of rdl in three mosquito disease vectors, recoding five amino acid residues in rdl of aedes aegypti a ...201729223796
chemosensory responses to the repellent nepeta essential oil and its major component nepetalactone by aedes aegypti (diptera: culicidae), a vector of zika virus.nepeta essential oil (neo; catnip) and its major component, nepetalactone, have long been known to repel insects including mosquitoes. however, the neural mechanisms through which these repellents are detected by mosquitoes, including the yellow fever mosquito aedes aegypti (l.), an important vector of zika virus, were poorly understood. here we show that neo volatiles activate olfactory receptor neurons within the basiconic sensilla on the maxillary palps of female ae. aegypti. a gustatory rece ...201728407077
quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection.zika virus (zikv) is an emerging arbovirus belonging to the genus flavivirus of the family flaviviridae during replication processes, flavivirus manipulates host cell systems to facilitate its replication, while the host cells activate antiviral responses. identification of host proteins involved in the flavivirus replication process may lead to the discovery of antiviral targets. the mosquitoes aedes aegypti and aedes albopictus are epidemiologically important vectors for zikv, and effective re ...201728404849
toxicological effects of sphaeranthus indicus linn. (asteraceae) leaf essential oil against human disease vectors, culex quinquefasciatus say and aedes aegypti linn., and impacts on a beneficial mosquito predator.use of environmentally friendly, decomposable natural products for effective vector control has gained considerable momentum in modern society. in this study, essential oil of sphaeranthus indicus (si-eo) was extracted and further phytochemical screening revealed fourteen compounds with prominent peak area percentage of 24.9 and 22.54% in 3,5-di-tert-butyl-4-hydroxybenzaldehyde and benzene,2-(1,1-dimethylethyl)-1,4-dimethoxy, respectively. the si-eo was further evaluated for their larvicidal res ...028455566
citrus seed oils efficacy against larvae of aedes aegypti.dengue fever is a serious public health issue in pakistan for many years. globally plants have been reported to contain compounds with insecticidal properties. these properties have been demonstrated more recently on the larval stages of mosquitoes. therefore, citrus cultivar seeds were evaluated for larvicidal potential against the primary dengue vector aedes aegypti.201729322059
field evaluation of indoor thermal fog and ultra-low volume applications for control of aedes aegypti in thailand.efficacies of a handheld thermal fogger (patriot™) and a backpack ultra-low volume (ulv) sprayer (twister™) with combinations of 2 different adulticides (pyrethrin, deltamethrin) and an insect growth regulator (pyriproxyfen) were field-tested and compared for their impact on reducing indoor aedes aegypti populations in thailand. the effectiveness of the indoor space sprays was evaluated by sampling the natural ae. aegypti population in houses and determining their physiological status, by monito ...201728590217
larvicidal potential of juglans regia male flower against anopheles stephensi, aedes aegypti and culex quinquefasciatus.the diseases vectored by mosquitoes continue to be a main cause of illnesses and death throughout the world. the methanol extract of juglans regia male flower was screened for larvicidal activity against three therapeutically important mosquito vectors viz., malarial vector, anopheles stephensi; dengue vector, aedes aegypti and the filarial vector, culex quinquefasciatus. the larvicidal activity was assayed against the early fourth-instar larvae of tested mosquito species at a concentration rang ...201729251995
relative insecticidal efficacy of three spatial repellent integrated light sources against aedes aegypti.three repellent products, off! mosquito lamp, insecticandel, and rescue decoshield, were comparatively evaluated against aedes aegypti in 130-m2 enclosed areas with a 317-m3 air volume. the results showed that the off! mosquito lamp with metofluthrin had a greater effect than the insecticandel with transfluthrin, which had greater effect than the decoshield with lemongrass oil and several other plant oils against ae. aegypti. the off! mosquito lamp was the only product to exceed 50% mortality. a ...201729369020
behavior of detoxifying enzymes of aedes aegypti exposed to girgensohnine alkaloid analog and cymbopogon flexuosus essential oil.because mosquito control depend on the use of commercial insecticides and resistance has been described in some of them, there is a need to explore new molecules no resistant. in vivo effects of girgensohnine analog 2-(3,4-dimethoxyphenyl)-2-(piperidin-1-yl)acetonitrile dppa and cymbopogon flexuosus essential oil cfeo, on the detoxifying enzymes acetylcholinesterase (ache), glutathione-s-transferase (gst), nonspecific esterases (α- and β-), mixed function oxidases (mfo) and p-npa esterases were ...201829129589
the attractiveness of the gravid aedes trap to dengue vectors in fiji.the gravid aedes trap (gat) is a passive trap that relies on visual and olfactory cues to lure and capture gravid mosquitoes. the gat was designed as a dengue vector surveillance tool for use in conditions where power sources are not readily available. experiments were conducted over a 10-d period in fiji to determine which species of mosquitoes the gat would collect, how different infusion types affect the attractiveness of the trap, how long each infusion type took to begin attracting mosquito ...201729244174
environmentally benign nanometric neem-laced urea emulsion for controlling mosquito population in environment.the increasing risk of vector-borne diseases and the environmental pollution in the day-to-day life due to the usage of the conventional pesticides makes the role of nanotechnology to come into the action. the current study deals with one of the applications of nanotechnology through the formulation of neem urea nanoemulsion (nune). nune was formulated using neem oil, tween 20, and urea using the microfluidization method. prior to the development of nanoemulsion, the ratio of oil/surfactant/urea ...201829116538
functional characterization of the dual allatostatin-a receptors in mosquitoes.the neuropeptide allatostatin-a (asta) and its cognate receptors (astars) are involved in the modulation of feeding behavior, which in hematophagous insects includes the regulation of the disease vector-related behaviors, host seeking and blood feeding. in mosquitoes and other dipterans, there are two copies of astar, contrasting with the single copy found in other insects. in this study, we identified and cloned the dual astar system of two important disease vectors aedes aegypti and culex quin ...201829103918
genome sequence of a novel strain of a phasi charoen-like virus identified in zhanjiang, china.here, we report the genome sequence of a novel strain of a phasi charoen-like virus, isolated from aedes aegypti mosquitoes caught in zhanjiang province of china. phylogenetic analysis suggested that this virus belongs to a new genus, goukovirus, in the family bunyaviridae this is the first reported genome sequence of a phasi charoen-like virus identified in china.201829326198
in silico validation of d7 salivary protein-derived b- and t-cell epitopes of aedes aegypti as potential vaccine to prevent transmission of flaviviruses and togaviruses to humans.mosquito (aedes aegyptii) salivary proteins play a crucial role in facilitating viral transmission from vector-to-host due to their role in facilitating the "blood meal" of the vector. three main proteins, d7, aegyptin and sialokinin play a role in this process. using in-silico programs, we identified b- and t-cell epitopes in the mosquito salivary proteins d7 long and short form. t-cell epitopes with high affinity to the most prevalent hla mhc class-i supertypes among different population group ...201729225429
the wolbachia strain wau provides highly efficient virus transmission blocking in aedes aegypti.introduced transinfections of the inherited bacteria wolbachia can inhibit transmission of viruses by aedes mosquitoes, and in ae. aegypti are now being deployed for dengue control in a number of countries. only three wolbachia strains from the large number that exist in nature have to date been introduced and characterized in this species. here novel ae. aegypti transinfections were generated using the walba and wau strains. in its native ae. albopictus, walba is maintained at lower density tha ...201829370307
a small-scale investigation into the effect of a larvicidal oil on oviposition site preference by aedes aegypti.larvicidal oils can be used to control immature aedes aegypti and other pestiferous mosquitoes. to test whether oil-based larvicides also act as oviposition deterrents, indoor and semi-field trials were conducted at the navy entomology center of excellence in jacksonville, fl. in both studies, treatment cages consisted of oviposition cups lined with seed germination paper as an oviposition substrate and filled with 1-wk-old southern live oak ( quercus virginiana) leaf litter-infused water. treat ...201729369032
comparison of adult mosquito abundance from biogents-2 sentinel and biogents gravid aedes traps in northeastern florida.we compared the efficacy of the biogents gravid aedes trap (bg-gat) and biogents-2 sentinel (bg2s) trap to assess differences in adult mosquito collection rates between these 2 mosquito surveillance methods in northeastern florida. species diversity of trap collections was significantly greater for bg2s than for bg-gat, with 14 mosquito species collected from the former trap and 4 species from the latter. aedes aegypti and culex quinquefasciatus were the 2 predominant species collected in both t ...201729369031
bg-sentinel™ trap efficacy as a component of proof-of-concept for push-pull control strategy for dengue vector mosquitoes.the efficacy of the bg-sentinel™ (bgs) trap as a "pull" component of a "push-pull" system (pps) for management of the dengue vector, aedes aegypti, was evaluated using local households in pu tuey, kanchanaburi, thailand. the pull component was the concluding phase of a 3-part investigation using a pps combination spatial repellent (sr) and bgs trap to capture adult vector mosquitoes. two sentinel households were selected for evaluation of bgs trap efficacy based on the highest pretrial indoor re ...201729369029
laboratory evaluation of synthetic blends of l-(+)-lactic acid, ammonia, and ketones as potential attractants for aedes aegypti.attraction of aedes aegypti to various binary, trinary, and quaternary blends of lactic acid and ketones with or without ammonia was studied using a dual choice olfactometer. a dose dependent attraction was observed in cases of single compounds where cyclopentanone attracted the highest percentage (36.9 ± 1.8%) of ae. aegypti when tested alone. no significant difference was observed between the attraction levels of trinary and binary blends of lactic acid and acetone or butanone when tested agai ...201729369028
multifaceted functional implications of an endogenously expressed trna fragment in the vector mosquito aedes aegypti.the mosquito aedes aegypti is the primary vector of human arboviral diseases caused by dengue, chikungunya and zika viruses. many studies have shown the potential roles of small rna molecules such as microrna, small interfering rna and piwi-interacting rna in vector mosquitoes. the function of trna fragments (trf), the newly discovered class of small rnas, in mosquitoes is not known. in this study, we show that specific trfs are expressed in significantly differential manner between males and fe ...201829364883
larvicidal activity against aedes aegypti and chemical characterization of the inflorescences of tagetes patula.the crude acetone extract (cae) of defatted inflorescences of tagetes patula was partitioned into five semipurified fractions: n-hexane (hf), dichloromethane (df), ethyl acetate (eaf), n-butanol (bf), and aqueous (aqf). bf was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to hsccc, where patuletin and patulitrin were isolated. cae and the fractions bf, eaf, df, and aqf were analyzed by lc-dad-ms, and patuletin and patulitrin were ...201729362590
[dengue, zika, chikungunya and the development of vaccines].dengue (denv), zika (zikv) and chikungunya (chikv), three arbovirosis transmitted by aedes mosquitoes, have spread in recent decades in humid tropical and subtropical zones. dengue is epidemic in subtropical areas of argentina. denv infection confers lasting immunity against the infecting serotype but increases the risk of serious disease upon reinfection by any of the other three. the recombinant tetravalent vaccine dengvaxia® prevents severe dengue and hospitalization in seropositive subjects. ...201829360072
cytoplasmic glutamine synthetase gene expression regulates larval development in bactrocera dorsalis (hendel).in insects, glutamine synthetase (gs), a key enzyme in the synthesis of glutamine, has been reported to be associated with embryonic development, heat shock response, and fecundity regulation. however, little is known about the influence of gs on postembryonic development. in this study, we demonstrate that blocking the activity of gs in the oriental fruit fly (bactrocera dorsalis) with use of a gs-specific inhibitor (l-methionine s-sulfoximine), led to a significant delay in larval development, ...201829359358
persistence of toxic activity of fermentation extracts from bacillus thuringiensis var. israelensis after more than three decades of storage.this study was carried out to determine the persistence of toxicity of fermentation extracts of bacillus thuringiensis var. israelensis after more than three decades of storage. for this purpose, a population of aedes aegypti was established. the mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified who protocol. the extracts with the highest mortality rate were determined in triplicat ...201729358951
fine-scale landscape genomics helps explain the slow spatial spread of wolbachia through the aedes aegypti population in cairns, australia.the endosymbiotic bacterium wolbachia suppresses the capacity for arbovirus transmission in the mosquito aedes aegypti, and can spread spatially through wild mosquito populations following local introductions. recent introductions in cairns, australia have demonstrated slower than expected spatial spread. potential reasons for this include: (i) barriers to ae. aegypti dispersal; (ii) higher incidence of long-range dispersal; and (iii) intergenerational loss of wolbachia. we investigated these th ...201829358725
current concerns and perspectives on zika virus co-infection with arboviruses and hiv.dissemination of vector-borne viruses, such as zika virus (zikv), in tropical and sub-tropical regions has a complicated impact on the immunopathogenesis of other endemic viruses such as dengue virus (denv), chikungunya virus (chikv) and human immunodeficiency virus (hiv). the consequences of the possible co-infections with these viruses have specifically shown significant impact on the treatment and vaccination strategies. zikv is a mosquito-borne flavivirus from african and asian lineages that ...201829352633
aerial ulv control of aedes aegypti with naled (dibrom) inside simulated rural village and urban cryptic habitats.we conducted aerial fixed wing ultra low volume (ulv) spray trials with naled to investigate penetration of exposed and simulated cryptic habitat within opened buildings, partially sealed buildings, and outdoor locations targeting sentinel adult aedes aegypti mosquitoes in north central florida. mortality was observed in open and closed buildings and outdoors, even in mosquitoes placed in cryptic habitats. observations on the impact of building type, mosquito exposure method such as placement in ...201829352307
reduction in musca domestica fecundity by dsrna-mediated gene knockdown.house flies (musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the u.s. non-target effects and widespread resistance challenge the efficacy of traditional chemical control. double stranded rna (dsrna) has been suggested as a biopesticide for m. domestica but a phenotypic response due to the induction of the rnai pathway has not been demonstrated in adults. in this study female house flies were injected with dsrna targeting actin-5c or ribo ...201829342168
zika virus in salivary glands of five different species of wild-caught mosquitoes from mexico.zika virus (zikv) is a mosquito-borne pathogen, and aedes aegypti has been identified as the main vector of the disease. other mosquito species in the aedes and culex genera have been suggested to have the potential for being competent vectors based on experimental exposition of mosquitoes to an infectious blood meal containing zikv. here, we report the isolation in cell culture of zikv obtained from different body parts of wild-caught female mosquitoes (ae. aegypti, ae. vexans, cx. quinquefasci ...201829339746
wolbachia-induced transcription factor gata4 suppresses ovary-specific genes blastoderm-specific protein 25d and imaginal disc growth factor.the endosymbiotic bacterium wolbachia infects a wide array of insect hosts and has been implicated in a range of biological modifications as a consequence of its infection. previously, it was shown that the transcription factor gata4 was significantly induced in wolbachia wmelpop-cla strain infected aedes aegypti whole mosquitoes and cells. here, we provide evidence that this induction also occurs in mosquito ovaries where the ovary-specific genes blastoderm-specific protein 25d (bsg25d) and ima ...201829336504
vibrational properties of bulk boric acid 2a and 3t polymorphs and their two-dimensional layers: measurements and density functional theory calculations.boric acid (h3bo3) is being used effectively nowadays in traps/baits for the management of aedes aegypti l. and aedes albopictus skuse species of mosquitoes, which are the main spreading vectors worldwide for diseases such as malaria, dengue, and zika. previously, we published results on the structural, electronic, and optical properties of its molecular triclinic h3bo3-2a and trigonal h3bo3-3t polymorphs within the framework of density functional theory (dft). because of the renewed importance ...201829328646
the discovery of arthropod-specific viruses in hematophagous arthropods: an open door to understanding the mechanisms of arbovirus and arthropod evolution?the discovery of an odd virus from hematophagous arthropods 40 years ago by stollar and thomas described cell fusing agent virus in cells derived from aedes aegypti mosquitoes. then came the report of kamiti river virus from ae. macintoshi in 1999, followed by worldwide reports of the discovery of other viruses of mosquitoes, ticks, and midges that replicate only in arthropods and not in vertebrates or in vertebrate cells. these viruses (now totaling at least 64 published) have genomes analogous ...201829324047
dengue hemorrhagic fever virus in saudi arabia: a review.dengue fever is a global disease with a spectrum of clinical manifestation ranging from mild febrile disease to a severe disease in the form of dengue hemorrhagic fever and dengue shock syndrome. dengue virus is one viral hemorrhagic fever that exists in the kingdom of saudi arabia in addition to alkhurma (alkhurma) hemorrhagic fever, chikungunya virus, crimean-congo hemorrhagic fever, and rift valley fever. the disease is limited to the western and south-western regions of saudi arabia, where a ...201829319426
qsar analysis of plant-derived compounds with larvicidal activity against zika aedes aegypti (diptera: culicidae) vector using freely available descriptors.we develop a qsar model for predicting the larvicidal activity of 60 plant-derived molecules against aedes aegypti l. (diptera: culicidae), vector of several diseases such as, dengue, yellow fever, chikungunya and zika virus. the balanced subsets method (bsm) based on k-means cluster analysis (k-mca) is employed to split the data set. the replacement method (rm) variable subset selection technique coupled with multivariable linear regression (mlr) proves to be successful for exploring 18,326 mol ...201829314584
krüppel homologue 1 acts as a repressor and an activator in the transcriptional response to juvenile hormone in adult mosquitoes.krüppel homologue 1 (kr-h1) is a zinc finger transcription factor that is upregulated in insects by juvenile hormone (jh) in metamorphosis and adult reproduction. the molecular function of kr-h1 in reproduction remains largely unknown. here we report that aakr-h1 functions as an important transcription regulator in adult female aedes aegypti mosquitoes. the amount of aakr-h1 protein increases with rising jh levels after adult emergence, reaches its peak at 48 h after eclosion, then decreases gra ...201829314423
aristololactam derivatives from the fruits of aristolochia contorta bunge.three new aristololactam derivatives, aristololactam w-y (1-3), and three known compounds (4-6) were isolated from the fruits of aristolochia contorta bunge. compounds 1 and 2 represent the first example of an n-ch2och3 aristololactam derivative from natural products. their structures were elucidated by 1d/2d nmr and hresims spectra. all of the isolated compounds were evaluated for their insecticidal activity against 4th instar larvae of aedes aegypti. compound 4 displayed insecticidal activity ...201829313366
the genomes of four meyerozymacaribbica isolates and novel insights into the meyerozymaguilliermondii species complex.yeasts of the meyerozyma guilliermondii species complex are widespread in nature and can be isolated from a variety of sources, from the environment to arthropods to hospital patients. to date, the species complex comprises the thoroughly studied and versatile m. guilliermondii, and the hard to distinguish m. caribbica, and candida carpophila here we report the whole-genome sequencing and de novo assembly of four m. caribbica isolates, identified with the most recent molecular techniques, derive ...201829311113
critical low temperature for the survival of aedes aegypti in taiwan.taiwan is geographically located in a region that spans both tropical and subtropical climates (22-25°n and 120-122°e). the taiwan centers for disease control have found that the ecological habitat of aedes aegypti appears only south of 23.5°n. low temperatures may contribute to this particular habitat distribution of ae. aegypti under the influence of the east asian winter monsoon. however, the threshold condition related to critically low temperatures remains unclear because of the lack of lar ...201829310716
synergistic effect of fertilizer and plant material combinations on the development of aedes aegypti (diptera: culicidae) and anopheles gambiae (diptera: culicidae) mosquitoes.chemical fertilizers are used everywhere, which often pollute the breeding sites of mosquitoes. in this laboratory study, the consequences on aedes aegypti (l.) (diptera: culicidae) and anopheles gambiae (giles) (diptera: culicidae) of water-containing plant matter (pm) alone, or in association with an npk type of fertilizer (pm+npk), were evaluated. to obtain a 20% imaginal emergence of an. gambiae (iet20), the bioassays carried out with pm have evidenced that its larvae need four times as much ...201729309617
assessment of a trap based aedes aegypti surveillance program using mathematical modeling.the goal of this study was to assess the goodness-of-fit of theoretical models of population dynamics of aedes aegypti to trap data collected by a long term entomological surveillance program. the carrying capacity k of this vector was estimated at city and neighborhood level. adult mosquito abundance was measured via adults collected weekly by a network of sticky traps (mosquitraps) from january 2008 to december 2011 in vitória, espírito santo, brazil. k was the only free parameter estimated by ...201829304070
hypoxia-induced transcription factor signaling is essential for larval growth of the mosquito aedes aegypti.gut microbes positively affect the physiology of many animals, but the molecular mechanisms underlying these benefits remain poorly understood. we recently reported that bacteria-induced gut hypoxia functions as a signal for growth and molting of the mosquito aedes aegypti in this study, we tested the hypothesis that transduction of a gut hypoxia signal requires hypoxia-induced transcription factors (hifs). expression studies showed that hif-α was stabilized in larvae containing bacteria that in ...201829298915
group b wolbachia strain-dependent inhibition of arboviruses.mosquito-borne viruses, including zika virus (zikv) and dengue virus (denv), are global threats that continue to infect millions annually. historically, efforts to combat the spread of these diseases have sought to eradicate the mosquito population. this has had limited success. recent efforts to combat the spread of these diseases have targeted the mosquito population and the mosquito's ability to transmit viruses by altering the mosquito's microbiome. the introduction of particular strains of ...201829297702
zika virus infection modulates the bacterial diversity associated with aedes aegypti as revealed by metagenomic analysis.zika is a re-emerging infection that has been considered a major threat to global public health. currently at least 100 countries are at risk of zika virus (zikv) transmission. aedes aegypti is the main mosquito vector in the americas. this vector is exposed to, and interacts symbiotically with a variety of microorganisms in its environment, which may result in the formation of a lifetime association. here, the unknown effect that zikv exerts on the dynamic bacterial community harbored by this m ...201829293631
insecticide treated curtains and residual insecticide treatment to control aedes aegypti: an acceptability study in santiago de cuba.within the context of a field trial conducted by the cuban vector control program (aacp), we assessed acceptability of insecticide-treated curtains (itcs) and residual insecticide treatment (rit) with deltamethrin by the community. we also assessed the potential influence of interviewees' risk perceptions for getting dengue and disease severity.201829293501
n-substituted methyl maleamates as larvicidal compounds against aedes aegypti (diptera: culicidae).severe human arboviral diseases can be transmitted by the mosquito aedes aegypti (l.), including dengue, chikungunya, zika, and yellow fever. the use of larvicides in containers that can result as potential breeding places and cannot be eliminated is the main alternative in control programs. however, their continuous and widespread use caused an increase in insecticide-resistant populations of this mosquito. the aim of this study was to evaluate the effect of three n-substituted methyl maleamate ...201829290037
dengue and yellow fever virus vectors: seasonal abundance, diversity and resting preferences in three kenyan cities.the transmission patterns of dengue (denv) and yellow fever (yfv) viruses, especially in urban settings, are influenced by aedes (stegomyia) mosquito abundance and behavior. despite recurrent dengue outbreaks on the kenyan coast, these parameters remain poorly defined in this and other areas of contrasting dengue endemicity in kenya. in assessing the transmission risk of denv/yfv in three kenyan cities, we determined adult abundance and resting habits of potential aedes (stegomyia) vectors in ki ...201729284522
meta-analysis of aedes aegypti expression datasets: comparing virus infection and blood-fed transcriptomes to identify markers of virus presence.the mosquito aedes aegypti (l.) is vector of several arboviruses including dengue, yellow fever, chikungunya, and more recently zika. previous transcriptomic studies have been performed to elucidate altered pathways in response to viral infection. however, the intrinsic coupling between alimentation and infection were unappreciated in these studies. feeding is required for the initial mosquito contact with the virus and these events are highly dependent. addressing this relationship, we reinterr ...201729376049
mosquito vector-associated microbiota: metabarcoding bacteria and eukaryotic symbionts across habitat types in thailand endemic for dengue and other arthropod-borne diseases.vector-borne diseases are a major health burden, yet factors affecting their spread are only partially understood. for example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. nonetheless, current knowledge of mosquito-associated microbial communities is limited. to characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas ...201829375803
[estimation of underreporting of chikungunya virus infection cases in girardot, colombia, from november, 2014, to may, 2015].chikungunya virus infection in colombia became epidemic in 2015. it is estimated that there is underreporting of cases to the public health surveillance system which can induce bias in epidemiological projections for decision making, a serious problem, as it veils the real magnitude and actual epidemiological importance of this disease.201729373771
genetically modified aedes aegypti to control dengue: a review.dengue is an acute infectious disease of viral etiology characterized by lymphadenopathy, leucopenia, headache, biphasic fever, pain in various parts of the body, rashes, and extreme physical weakness. it is a vector-borne disease caused by a positive-stranded rna virus of the family flaviviridae, genus flavivirus. dengue inflicts a significant health, economic, and social burden on populations of endemic areas. dengue virus is transmitted to humans by the mosquito vector aedes aegypti. vaccines ...201729283327
family level variation in wolbachia-mediated dengue virus blocking in aedes aegypti.the mosquito vector aedes aegypti is responsible for transmitting a range of arboviruses including dengue (denv) and zika (zikv). the global reach of these viruses is increasing due to an expansion of the mosquito's geographic range and increasing urbanization and human travel. vector control remains the primary means for limiting these diseases. wolbachia pipientis is an endosymbiotic bacterium of insects that has the ability to block the replication of pathogens, including flaviviruses such as ...201729282144
african and asian zika virus isolates display phenotypic differences both in vitro and in vivo.zika virus (zikv) is a mosquito-borne member of the genus flavivirus that has emerged since 2007 to cause outbreaks in africa, asia, oceania, and most recently, in the americas. here, we used an isolate history as well as genetic and phylogenetic analyses to characterize three low-passage isolates representing african (ard 41525) and asian (cpc-0740, sv0127-14) lineages to investigate the potential phenotypic differences in vitro and in vivo. the african isolate displayed a large plaque phenotyp ...201729280428
field- and clinically derived estimates of wolbachia-mediated blocking of dengue virus transmission potential in aedes aegypti mosquitoes.the wmel strain of wolbachia can reduce the permissiveness of aedes aegypti mosquitoes to disseminated arboviral infections. here, we report that wmel-infected ae. aegypti (ho chi minh city background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (denv) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. the wmel-infected mosquitoes that are field-reared have even greater relative resistance to denv infection ...201829279375
altitudinal distribution of aedes indices during dry season in the dengue endemic area of central java, indonesiaaedes mosquitoes, mainly aedes aegypti and aedes albopictus, are the primary and secondary vectors of dengue viruses in indonesia, with transmission occurring by sucking blood. the density of the vectors is influenced by season and rainfall, but limited by altitude. the aim of the study is to describe the density and distribution of dengue vectors during the dry season based on the altitudes of recent dengue cases in five regencies of central java province, indonesia. mosquito larvae and pupae w ...201729274215
zika virus: an emerging infectious disease with serious perinatal and neurologic complications.zika virus (zikv) is a flavivirus that is primarily transmitted by aedes aegypti, the mosquito vector also important in the transmission of the flaviviruses responsible for dengue fever, yellow fever and chikungunya. due to occurrence in the same geographic regions, serological cross-reactivity, and similar, albeit often less severe clinical manifestations as dengue and chikungunya infections, zikv infection likely went undetected and/or misdiagnosed for many years. zikv is somewhat unique among ...201729273403
history and emergence of zika virus.zika virus was discovered in east africa in 1947 by the rockefeller foundation during investigations on the ecology of yellow fever. although it was subsequently shown to have widespread distribution in africa and asia, it was not known to cause epidemics until 2007. this paper describes the history of the virus discovery, emergence and evolution as an epidemic virus, and the its evolving clinical spectrum.201729267917
zika virus mosquito vectors: competence, biology, and vector control.zika virus (zikv) (flaviviridae, flavivirus) has become one of the most medically important mosquito-borne viruses because of its ability to cause microcephaly in utero and guillain-barré syndrome in adults. this virus emerged from its sylvatic cycle in africa to cause an outbreak in yap, federated states of micronesia in 2007, french polynesia in 2014, and most recently south america in 2015. the rapid expansion of zikv in the americas largely has been due to the biology and behavior of its vec ...201729267910
at the origin of a worldwide invasion: unraveling the genetic makeup of the caribbean bridgehead populations of the dengue vector aedes aegypti.human-driven global environmental changes have considerably increased the risk of biological invasions, especially the spread of human parasites and their vectors. among exotic species that have major impacts on public health, the dengue fever mosquito aedes aegypti originating from africa has spread worldwide during the last three centuries. although considerable progress has been recently made in understanding the history of this invasion, the respective roles of human and abiotic factors in s ...201829267872
chikungunya: molecular aspects, clinical outcomes and pathogenesis.the alarming worldwide emergence of the chikungunya virus began in the last decade. since the first autochthonous transmission in mexico in november of 2014, the virus has spread throughout the country, resulted in multiple outbreaks. this virus produces an acute and self-limiting disease characterized by fever, polyarthralgia, myalgia, exanthema, and general malaise. it is transmitted to humans by the bite of aedes aegypti and a. albopictus mosquitoes. the fact that the clinical presentation is ...201729265115
characteristics of aedes aegypti adult mosquitoes in rural and urban areas of western and coastal kenya.aedes aegypti is the main vector for yellow fever, dengue, chikungunya and zika viruses. recent outbreaks of dengue and chikungunya have been reported in kenya. presence and abundance of this vector is associated with the risk for the occurrence and transmission of these diseases. this study aimed to characterize the presence and abundance of ae. aegypti adult mosquitoes from rural and urban sites in western and coastal regions of kenya. presence and abundance of ae. aegypti adult mosquitoes wer ...201729261766
individual co-variation between viral rna load and gene expression reveals novel host factors during early dengue virus infection of the aedes aegypti midgut.dengue virus (denv) causes more human infections than any other mosquito-borne virus. the current lack of antiviral strategies has prompted genome-wide screens for host genes that are required for denv infectivity. earlier transcriptomic studies that identified denv host factors in the primary vector aedes aegypti used inbred laboratory colonies and/or pools of mosquitoes that erase individual variation. here, we performed transcriptome sequencing on individual midguts in a field-derived ae. aeg ...201729261661
assessment of maldi-tof mass spectrometry for filariae detection in aedes aegypti mosquitoes.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is an emerging tool for routine identification of bacteria, archaea and fungi. it has also been recently applied as an accurate approach for arthropod identification. preliminary studies have shown that the maldi-tof ms was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and plasmodium parasites, respectively. the aim of the present study was to test the efficien ...201729261659
origin of a high-latitude population of aedes aegypti in washington, dc.an overwintering population of aedes aegypti has been documented in the capitol hill neighborhood of washington, dc, since 2011. mitochondrial cytochrome oxidase i (mtcoi) sequence data presented in a previous study traced the origin to the new world. here, we use microsatellite and snp chip data along with more mitochondrial dna (mtdna) sequences on washington ae. aegypti samples and samples from potential sources to further narrow the origin of this population. genetically, washington ae. aegy ...201729260658
from ground pools to treeholes: convergent evolution of habitat and phenotype in aedes mosquitoes.invasive mosquito species are responsible for millions of vector-borne disease cases annually. the global invasive success of aedes mosquitoes such as aedes aegypti and aedes albopictus has relied on the human transport of immature stages in container habitats. however, despite the importance of these mosquitoes and this ecological specialization to their widespread dispersal, evolution of habitat specialization in this group has remained largely unstudied. we use comparative methods to evaluate ...201729258425
zika virus exhibits lineage-specific phenotypes in cell culture, in aedes aegypti mosquitoes, and in an embryo model.zika virus (zikv) has quietly circulated in africa and southeast asia for the past 65 years. however, the recent zikv epidemic in the americas propelled this mosquito-borne virus to the forefront of flavivirus research. based on historical evidence, zikv infections in africa were sporadic and caused mild symptoms such as fever, skin rash, and general malaise. in contrast, recent asian-lineage zikv infections in the pacific islands and the americas are linked to birth defects and neurological dis ...201729258204
seasonal and spatial quantitative changes in aedes aegypti under distinctly different ecological areas of lahore, pakistan.to find out the variations in larval and adult density of aedes aegypti in different seasons under different ecological conditions.201729256519
social-ecological factors and preventive actions decrease the risk of dengue infection at the household-level: results from a prospective dengue surveillance study in machala, ecuador.in ecuador, dengue virus (denv) infections transmitted by the aedes aegypti mosquito are among the greatest public health concerns in urban coastal communities. community- and household-level vector control is the principal means of controlling disease outbreaks. this study aimed to assess the impact of knowledge, attitudes, and practices (kaps) and social-ecological factors on the presence or absence of denv infections in the household.201729253873
an approach of the exact linearization techniques to analysis of population dynamics of the mosquito aedes aegypti.dengue fever, chikungunya and zika are caused by different viruses and mainly transmitted by aedes aegypti mosquitoes. these diseases have received special attention of public health officials due to the large number of infected people in tropical and subtropical countries and the possible sequels that those diseases can cause. in severe cases, the infection can have devastating effects, affecting the central nervous system, muscles, brain and respiratory system, often resulting in death. vaccin ...201729253493
aedes aegypti resistance development to commonly used insecticides in jakarta, indonesia.aedes aegypti is the primary vector of various relevant arthropod-borne viral infectious diseases worldwide. the mosquito control is still mainly performed by using insecticides but their effectiveness is increasingly questioned nowadays. we here conducted a study on ae. aegypti resistance development towards several commonly used insecticides in the capital city of jakarta, indonesia. in order to achieve this goal, ae. aegypti eggs from jakarta were collected with ovitraps and hatched in the in ...201729253003
green synthesis of silver nanoparticles using holarrhena antidysenterica (l.) wall.bark extract and their larvicidal activity against dengue and filariasis vectors.the present study was carried out to evaluate the larvicidal potential of methanol, hexane, acetone, chloroform, and aqueous bark extracts of holarrhena antidysenterica (l.) wall. and silver nanoparticles (agnps) synthesized using aqueous bark extract against the third instar larvae of aedes aegypti l. and culex quinquefasciatus say. agnps were prepared by adding 10 ml of aqueous bark extract in 90 ml of 1 mm silver nitrate (agno3) solution. after 5 min of mixing, a change in color from yellow t ...201829250727
aedes aegypti microrna mir-2b regulates ubiquitin-related modifier to control chikungunya virus replication.arboviruses that replicate in mosquitoes activate innate immune response within mosquitoes. regulatory non-coding micrornas (mirna) are known to be modulated in mosquitoes during chikungunya infection. however, information about targets of these mirnas is scant. the present study was aimed to identify and analyze targets of mirnas that are regulated during chikungunya virus (chikv) replication in aedes aegypti cells and in the mosquito. employing next-generation sequencing technologies, we ident ...201729247247
zika virus can be venereally transmitted between aedes aegypti mosquitoes.alternative transmission routes have been described for zika virus (zikv). here, we assessed for the first time the venereal transmission of zikv between aedes aegypti under laboratory conditions.201729246237
nanotitania crystals induced efficient photocatalytic color degradation, antimicrobial and larvicidal activity.textile industries release tonnes of harmful toxic dyes into the environment, causing severe effects on living organisms, including humans. mosquitoes vectors spread important diseases which cause millions of human deaths worldwide. to control mosquitoes a number of synthetic mosquitocidal agents have been employed but all these pesticides pose harmful effects to human health and non-target species and also led to resistance development in treated vectors. microbial strains are also developing r ...201829241121
analytical report of the 2016 dengue outbreak in córdoba city, argentina.after elimination of the aedes aegypti vector in south america in the 1960s, dengue outbreaks started to reoccur during the 1990s; strongly in argentina since 1998. in 2016, córdoba city had the largest dengue outbreak in its history. in this article we report this outbreak including spatio-temporal analysis of cases and vectors in the city. a total of 653 dengue cases were recorded by the laboratory-based dengue surveillance system and georeferenced by their residential addresses. case maps wer ...201729239555
Displaying items 13401 - 13500 of 14974