Publications

[the effect of groups in tests on insecticide resistance in aedes aegypti].		5303669
heterogeneity in the trinidad ddt-resistant strain and the qs susceptible strain of aedes aegypti l. the isolation of highly resistant and highly susceptible substrains.	investigating heterogeneity in response to ddt with 2 strains of aedes aegypti, the author has compared ddt-resistance in the f(1) and f(2) progenies of single-pair matings of resistant with resistant (trinidad) and susceptible with susceptible (qs) strains.both strains showed heterogeneity; however, there was little overlap between the most tolerant qs progeny and the least resistant trin progeny. a substrain of very high resistance was isolated from a single trin progeny and one of very low to ...	5304437
mechanisms and inheritance of resistance and selection of resistance potential in aedes aegypti.		5299454
factors affecting the oviposition of aedes aegypti.		5299458
the need for standard and other strains of aedes aegypti for research.		5299455
measures to be taken to prevent the transmission of aedes aegypti by sea and air.		5299469
organization and administration of aedes aegypti control and eradication programmes.		5299462
current status of the formal genetics of aedes aegypti.		5299452
dynamics of aedes aegypti distribution and density. seasonal fluctuations in the americas.		5299446
dynamics of aedes aegypti distribution, density and seasonal prevalence in the mediterranean area.		5299448
aedes aegypti in the western pacific region.		5299449
insecticides for use against aedes aegypti.		5299464
the distribution, density and seasonal prevalence of aedes aegypti in west africa.		5299447
aedes aegypti and yellow fever.		5299444
mating, resting habits and dispersal of aedes aegypti.		5299459
aedes aegypti and other mosquitos in relation to the dengue syndrome.		4383543
[insecticide-resistance in aedes aegypti and allied species].		5299453
genetic control of aedes aegypti.		5299738
frmal genetics and cytogenetics of aedes aegypti.		5299451
the control of aedes aegypti in south-east asia and the western pacific.		5299463
taxonomy of aedes aegypti and related species.		4383544
possible use of the sterile-male technique for control of aedes aegypti.		5299467
cytogenetics of aedes aegypti.		5299736
biotic factors in the control of aedes aegypti.		5299466
toxic hazards from the use of insecticides in the control of aedes aegypti.		5299465
history of aedes aegypti eradication in the americas.		5299460
the use of discriminating concentrations in genetical studies on ddt-resistance in larval aedes aegypti l.		5299759
ddt-resistance in west african and asian strains of aedes aegypti (l.).	the yellow fever mosquito aedes aegypti has developed resistance to ddt in the caribbean region and in south-east asia, but not in west africa. therefore west african strains were compared with south-east asian strains for their response to laboratory selection with ddt. it was found that west african strains were much slower to respond initially, but eventually could build up a high degree of ddt-resistance. by crossing and backcrossing with a susceptible marker-gene strain, it was found that t ...	5299673
a soluble deoxyribonucleic acid in the mosquito aedes aegypti.		5227670
the distribution of aedes aegypti in thailand.		20604280
report on a pilot study of aedes aegypti control in bangkok.		20604289
insecticide-resistance studies on aedes aegypti in thailand.		20604290
the control of aedes aegypti.		20604288
diagnostic dosages to separate genotypes of aedes aegypti for ddt-resistance and dieldrin-resistance.		5296139
dehydrochlorination and ddt-resistance in culex mosquitos.	the mechanism of resistance to ddt was investigated in culex mosquitos to ascertain whether it was associated with detoxication to dde as in houseflies and aedes aegypti. resistant larvae of c. tarsalis were found to effect this dehydrochlorination, whereas the susceptible larvae did not; resistant larvae of c. fatigans completely converted all the absorbed ddt to the metabolite dde. enzyme assays in vitro showed that the resistant c. fatigans had 10 times the dehydrochlorinating activity, and r ...	14315723
toxicities of certain larvicides to resistant and susceptible aedes aegypti (l.).	in a study of the toxicological characteristics of dieldrin-resistant and ddt-resistant strains of aedes aegypti, combined with an evaluation of certain larvicides, 14 cyclodienes, 13 ddt-type compounds, 18 organophosphorus compounds, several carbamates and a number of other compounds were tested against larvae of a. aegypti. telodrin and gc-9160 proved to be toxic against a highly dieldrin-resistant strain. against highly ddt-resistant strains the toxicity of ddt could be enhanced by piperonyl ...	5294255
yolk protein uptake in the oocyte of the mosquito aedes aegypti. l.	yolk proteins are thought to enter certain eggs by a process akin to micropinocytosis but the detailed mechanism has not been previously depicted. in this study the formation of protein yolk was investigated in the mosquito aedes aegypti l. ovaries were fixed in phosphate-buffered osmium tetroxide, for electron microscopy, before and at intervals after a meal of blood. the deposition of protein yolk in the oocyte was correlated with a 15-fold increase in 140 mmicro pit-like depressions on the oo ...	14126875
the aseptic rearing of aedes aegypti.		14274211
experimental observations governing the choice of a test method for determining the ddt-irritability of adult mosquitos.	in an attempt to determine the optimum test conditions for assessing the irritability of adult mosquitos to ddt, the author has carried out a series of experiments with two strains of aedes aegypti, one resistant and one susceptible to ddt. in these experiments a comparison was made between, on the one hand, the results obtained with the three possible test steps included in a tentative method proposed in 1960 by the who expert committee on insecticides, and, on the other hand, the results with ...	14122447
methods for mass rearing of aedes aegypti (l.).		14049192
antimetabolites in the nutrition of aedes aegypti l. larvae; nicotinic acid analogues.		14011576
the elimination of urban yellow fever in the americas through the eradication of aedes aegypti.		13978257
toxicity of aromatic acids to the larvae of the mosquito aedes aegypti l. and the counteracting influence of amino acids.		14351182
molecular analysis of juvenile hormone analog action in controlling the metamorphosis of the red flour beetle, tribolium castaneum.	the juvenile hormone analogs (jha) are known to disrupt insect development but the molecular mechanisms of their action have been studied only in a few model insects belonging to orders diptera and lepidoptera. here, we investigated the mechanisms of jha action in red flour beetle, tribolium castaneum, belonging to the order coleoptera. application of jha during penultimate and final instar larval stages blocked larval-pupal metamorphosis and induced supernumerary larval molts. when compared to ...	19072925
screening of uruguayan plants for deterrent activity against insects.	we evaluated the anti-insectan activity of extracts from different vegetative parts of ten plant species native to uruguay. the selected plants belong to five families: bignoniaceae: clytostoma callistegioides, dolichandra cynanchoides, macfadyena unguis-cati; sapindaceae: dodonaea viscosa, allophylus edulis, serjania meridionalis; lamiaceae: salvia procurrens, salvia guaranitica; solanaceae: lycium cestroides; and phytolaccaceae: phytolacca dioica. the extracts were evaluated in independent bio ...	20046902
aversion and attraction through olfaction.	sensory cues that predict reward or punishment are fundamental drivers of animal behavior. for example, attractive odors of palatable food or a potential mate predict reward, while aversive odors of pathogen-laced food or a predator predict punishment. aversive and attractive odors can be detected by intermingled sensory neurons that express highly related olfactory receptors and display similar central projections. these findings raise basic questions of how innate odor valence is extracted fro ...	25649823
chikungunya on the move.	in december 2013, chikungunya virus (chikv) transmission was reported for the first time in the americas. since then it has spread quickly, with more than 1 million suspected and confirmed cases being reported in one year, where previously there were only sporadic travel-related cases. transmission patterns suggest that the epidemic in the southern hemisphere is only beginning and that chikungunya will not go away anytime soon.	25649340
awareness and support of release of genetically modified "sterile" mosquitoes, key west, florida, usa.	after a dengue outbreak in key west, florida, during 2009-2010, authorities, considered conducting the first us release of male aedes aegypti mosquitoes genetically modified to prevent reproduction. despite outreach and media attention, only half of the community was aware of the proposal; half of those were supportive. novel public health strategies require community engagement.	25625795
use of insecticide-treated house screens to reduce infestations of dengue virus vectors, mexico.	dengue prevention efforts rely on control of virus vectors. we investigated use of insecticide-treated screens permanently affixed to windows and doors in mexico and found that the screens significantly reduced infestations of aedes aegypti mosquitoes in treated houses. our findings demonstrate the value of this method for dengue virus vector control.	25625483
integrating participatory community mobilization processes to improve dengue prevention: an eco-bio-social scaling up of local success in machala, ecuador.	this project investigates the effectiveness and feasibility of scaling-up an eco-bio-social approach for implementing an integrated community-based approach for dengue prevention in comparison with existing insecticide-based and emerging biolarvicide-based programs in an endemic setting in machala, ecuador.	25604763
effectiveness and feasibility of long-lasting insecticide-treated curtains and water container covers for dengue vector control in colombia: a cluster randomised trial.	long-lasting insecticide-treated net (llin) window and door curtains alone or in combination with llin water container covers were analysed regarding effectiveness in reducing dengue vector density, and feasibility of the intervention.	25604762
long-lasting insecticide-treated house screens and targeted treatment of productive breeding-sites for dengue vector control in acapulco, mexico.	long-lasting insecticidal net screens (llis) fitted to domestic windows and doors in combination with targeted treatment (tt) of the most productive aedes aegypti breeding sites were evaluated for their impact on dengue vector indices in a cluster-randomised trial in mexico between 2011 and 2013.	25604761
entomological impact and social participation in dengue control: a cluster randomized trial in fortaleza, brazil.	this study intended to implement a novel intervention strategy, in brazil, using an ecohealth approach and analyse its effectiveness and costs in reducing aedes aegypti vector density as well as its acceptance, feasibility and sustainability. the intervention was conducted from 2012 to 2013 in the municipality of fortaleza, northeast brazil.	25604760
improved dengue fever prevention through innovative intervention methods in the city of salto, uruguay.	uruguay is located at the southern border of aedes aegypti distribution on the south american sub-continent. the reported dengue cases in the country are all imported from surrounding countries. one of the cities at higher risk of local dengue transmission is salto, a border city with heavy traffic from dengue endemic areas.	25604764
evaluation of the who classification of dengue disease severity during an epidemic in 2011 in the state of ceará, brazil.	in 2009, the world health organization (who) issued a new guideline that stratifies dengue-affected patients into severe (sd) and non-severe dengue (nsd) (with or without warning signs). to evaluate the new recommendations, we completed a retrospective cross-sectional study of the dengue haemorrhagic fever (dhf) cases reported during an outbreak in 2011 in northeastern brazil. we investigated 84 suspected dhf patients, including 45 (53.6%) males and 39 (46.4%) females. the ages of the patients r ...	24626308
disruption of dengue virus transmission by mosquitoes.	current control efforts for mosquito-borne arboviruses focus on mosquito control involving insecticide applications, which are becoming increasingly ineffective and unsustainable in urban areas. mosquito population replacement is an alternative arbovirus control concept aiming at replacing virus-competent vector populations with laboratory-engineered incompetent vectors. a prerequisite for this strategy is the design of robust anti-pathogen effectors that can ultimately be genetically driven thr ...	26120563
wolbachia endosymbiont of brugia malayi elicits a t helper type 17-mediated pro-inflammatory immune response through wolbachia surface protein.	wolbachia is an endosymbiotic bacterium of the filarial nematode brugia malayi. the symbiotic relationship between wolbachia and its filarial host is dependent on interactions between the proteins of both organisms. however, little is known about wolbachia proteins that are involved in the inflammatory pathology of the host during lymphatic filariasis. in the present study, we cloned, expressed and purified wolbachia surface protein (r-wsp) from wolbachia and administered it to mice, either alon ...	25059495
voltage coupling of primary h+ v-atpases to secondary na+- or k+-dependent transporters.	this review provides alternatives to two well established theories regarding membrane energization by h(+) v-atpases. firstly, we offer an alternative to the notion that the h(+) v-atpase establishes a protonmotive force (pmf) across the membrane into which it is inserted. the term pmf, which was introduced by peter mitchell in 1961 in his chemiosmotic hypothesis for the synthesis of atp by h(+) f-atp synthases, has two parts, the electrical potential difference across the phosphorylating membra ...	19448072
a lepidopteran orthologue of reaper reveals functional conservation and evolution of iap antagonists.	genetic studies in drosophila melanogaster have revealed that inhibitor of apoptosis (iap) proteins and iap antagonists such as reaper play a pivotal role in controlling cell death in insects. interestingly, although the sequences and structures of iaps are highly conserved, the sequence of iap antagonists diverged very rapidly during evolution, making their identification difficult. using a customized bioinformatics approach, we identified an iap antagonist, iap-binding motif 1 (ibm1), from the ...	19523066
chloroquine use improves dengue-related symptoms.	dengue is the most important arboviral disease in the world. as chloroquine, an antimalarial agent, has shown some antiviral effects, this study evaluated its effect in patients with dengue. a randomised, double-blind study was performed by administering chloroquine or placebo for three days to 129 patients with dengue-related symptoms. of these patients, 37 were confirmed as having dengue and completed the study; in total, 19 dengue patients received chloroquine and 18 received placebo. there w ...	23903975
influence of age and nutritional status on flight performance of the asian tiger mosquito aedes albopictus (diptera: culicidae).	the asian tiger mosquito, aedes albopictus, is a competent vector for arboviruses and recently was implicated as the vector of the first autochthonous cases of dengue and chikungunya in southern europe. the objective of this study was to analyze the flight performance of female ae. albopictus of different ages that were starved, sugar-fed, or sugar-fed and blood-fed, using flight mills. after three days of starvation post emergence, females flew an average distance of 0.7 ± 0.5 km in 1.9 ± 1.5 h ...	24404384
development associated profiling of chitinase and microrna of helicoverpa armigera identified chitinase repressive microrna.	expression of chitinase is developmentally regulated in insects in consonance with their molting process. during the larval-larval metamorphosis in helicoverpa armigera, chitinase gene expression varies from high to negligible. in the five-day metamorphic course of fifth-instar larvae, chitinase transcript is least abundant on third day and maximal on fifth day. microrna library prepared from these highest and lowest chitinase-expressing larval stages resulted in isolation of several mirnas. in ...	23887392
oral delivery of double-stranded rna in larvae of the yellow fever mosquito, aedes aegypti: implications for pest mosquito control.	rna interference has already proven itself to be a highly versatile molecular biology tool for understanding gene function in a limited number of insect species, but its widespread use in other species will be dependent on the development of easier methods of double-stranded rna (dsrna) delivery. this study demonstrates that rna interference can be induced in the mosquito aedes aegypti l. (diptera: culicidae) simply by soaking larvae in a solution of dsrna for two hours. the mrna transcripts for ...	24224468
acute gingival bleeding as a complication of dengue hemorrhagic fever.	dengue fever is mosquito borne disease caused by dengue virus (denv) of flaviviridae family. the clinical manifestations range from fever to severe hemorrhage, shock and death. here, we report a case of 20-year-old male patient undergoing orthodontic treatment presenting with acute gingival bleeding with a history of fever, weakness, backache, retro orbital pain and ecchymosis over his right arm. the hematological investigations revealed anemia, thrombocytopenia and positive dengue non-structura ...	24174736
rna interference in colorado potato beetle: steps toward development of dsrna as a commercial insecticide.	colorado potato beetle (cpb) is a notorious pest on potatoes and has a remarkable ability to detoxify plant chemicals and develop resistance against insecticides. dsrna targeting cpb genes could be expressed in potato plants to control this pest. however, previous attempts at introducing transgenic potato plants to control cpb were not highly successful. recent studies showed that feeding dsrna expressed in bacteria works very well to kill cpb. to realize the potential of rnai to control this an ...	26705514
she's a femme fatale: low-density larval development produces good disease vectors.	two hypotheses for how conditions for larval mosquitoes affect vectorial capacity make opposite predictions about the relationship of adult size and frequency of infection with vector-borne pathogens. competition among larvae produces small adult females. the competition-susceptibility hypothesis postulates that small females are more susceptible to infection and predicts frequency of infection should decrease with size. the competition-longevity hypothesis postulates that small females have low ...	25591112
chemical and biological insecticides select distinct gene expression patterns in aedes aegypti mosquito.	worldwide evolution of mosquito resistance to chemical insecticides represents a major challenge for public health, and the future of vector control largely relies on the development of biological insecticides that can be used in combination with chemicals (integrated management), with the expectation that populations already resistant to chemicals will not become readily resistant to biological insecticides. however, little is known about the metabolic pathways affected by selection with chemic ...	25540155
peripheral olfactory signaling in insects.	olfactory signaling is a crucial component in the life history of insects. the development of precise and parallel mechanisms to analyze the tremendous amount of chemical information from the environment and other sources has been essential to their evolutionary success. considerable progress has been made in the study of insect olfaction fueled by bioinformatics- based utilization of genomics along with rapid advances in functional analyses. here we review recent progress in our rapidly emergin ...	25584200
the enigmatic reception of deet - the gold standard of insect repellents.	repellents are important prophylactic tools for travelers and populations living in endemic areas of malaria, dengue, encephalitis, and other vector-borne diseases. deet is a safe, broad spectrum repellent, which provides complete protection over a long period of time. despite its low cost, more affordable alternatives are highly desirable, particularly for those in endemic areas where cost is an impediment. alternative compounds like ir 3535 and picaridin have been developed using molecular mod ...	25530943
evolution of mosquito preference for humans linked to an odorant receptor.	female mosquitoes are major vectors of human disease and the most dangerous are those that preferentially bite humans. a 'domestic' form of the mosquito aedes aegypti has evolved to specialize in biting humans and is the main worldwide vector of dengue, yellow fever, and chikungunya viruses. the domestic form coexists with an ancestral, 'forest' form that prefers to bite non-human animals and is found along the coast of kenya. we collected the two forms, established laboratory colonies, and docu ...	25391959
dengue: "plus ça change, plus c'est la même chose".		24600111
making designer mutants in model organisms.	recent advances in the targeted modification of complex eukaryotic genomes have unlocked a new era of genome engineering. from the pioneering work using zinc-finger nucleases (zfns), to the advent of the versatile and specific talen systems, and most recently the highly accessible crispr/cas9 systems, we now possess an unprecedented ability to analyze developmental processes using sophisticated designer genetic tools. in this review, we summarize the common approaches and applications of these s ...	25336735
shotgun analysis on the peritrophic membrane of the silkworm bombyx mori.	the insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (pm), which facilitates food digestion and protects the gut epithelium. we used gel electrophoresis and mass spectrometry to identify the extracted proteins from the silkworm pm to obtain an in-depth understanding of the biological function of the silkworm pm components. a total of 305 proteins, with molecular weights ranging from 8.02 kda to 788.52 kda and the isoelectric points ...	23187007
studies on the post-oviposition blood-feeding behaviour of aedes aegypti (l.) (diptera: culicidae) in the laboratory.	the blood-feeding behaviour of the trinidad strain of aedes aegypti was studied, under laboratory conditions, using one female per cage and monitoring blood feeding immediately, 12, and 24 hours after oviposition. to get large numbers of females that had newly completed their first gonotrophic cycle, the diel oviposition periodicity was conducted using single females per cage and monitoring at 2-hour intervals. the diel oviposition periodicity showed a small morning peak (8%) during the first 2 ...	23265613
silencing of the hsf gene, the transcriptional regulator of a. gambiae male accessory glands, inhibits the formation of the mating plug in mated females and disrupts their monogamous behaviour.	discovering the molecular factors that shape the mating behaviour and the fertility of the mosquito anopheles gambiae, the principal vector of human malaria, is regarded as critical to better understand its reproductive success as well as for identifying new leads for malaria control measures. in a. gambiae mating induces complex behavioural and physiological changes in the females, including refractoriness to subsequent mating and induction of egg-laying. in other insects including drosophila a ...	23265612
efficacy of three formulations of diflubenzuron, an insect growth regulator, against culex quinquefasciatus say, the vector of bancroftian filariasis in india.	insect growth regulators (igrs) offer alternatives to conventional chemical larvicides that pose problem of resistance and environmental safety. however, only a limited number of igrs have been approved for use in mosquito control. in the present study, two new formulations of the igr diflubenzuron, 2 per cent granular (gr) and 2 per cent tablet (dt) were tested for its efficacy against culex quinquefasciatus, in comparison to its 25 per cent wettable powder (wp) formulation.	23287125
need for an efficient adult trap for the surveillance of dengue vectors.	the emergence and re-emergence of arboviral diseases transmitted by aedes aegypti and ae. albopictus continue to be a major threat in the tropics and subtropics. associations between currently used indices and dengue transmission have not been proven to be satisfactorily predictive of dengue epidemics. classical larval indices in dengue surveillance have limited use in assessing transmission risk and are a poor proxy for measuring adult emergence. besides, collection of larval indices is labour ...	23287120
seroprevalence of antibodies against chikungunya, dengue, and rift valley fever viruses after febrile illness outbreak, madagascar.	in october 2009, two-3 months after an outbreak of a febrile disease with joint pain on the eastern coast of madagascar, we assessed serologic markers for chikungunya virus (chikv), dengue virus (denv), and rift valley fever virus (rvfv) in 1,244 pregnant women at 6 locations. in 2 eastern coast towns, igg seroprevalence against chikv was 45% and 23%; igm seroprevalence was 28% and 5%. igg seroprevalence against denv was 17% and 11%. no anti-denv igm was detected. at 4 locations, 450-1,300 m hig ...	23092548
sources of dengue viruses imported into queensland, australia, 2002-2010.	to assess risk for importation of dengue virus (denv) into queensland, australia, and sources of imported viruses, we sequenced the envelope region of denv isolates from symptomatic patients with a history of travel during 2002-2010. the number of imported dengue cases greatly increased over the surveillance period, some of which were associated with domestic outbreaks. patients reported traveling to (in order) asia, papua new guinea, pacific island countries, and non-asia-pacific countries. by ...	23092682
mathematical models as aids for design and development of experiments: the case of transgenic mosquitoes.	we demonstrate the utility of models as aids in the design and development of experiments aimed at measuring the effects of proposed vector population control strategies. we describe the exploration of a stochastic, age-structured model that simulates field cage experiments that test the ability of a female-killing strain of the mosquito aedes aegypti (l.) to suppress a wild-type population. model output predicts that choices of release ratio and population size can impact mean extinction time a ...	23270145
gene flow networks among american aedes aegypti populations.	the mosquito aedes aegypti, the dengue virus vector, has spread throughout the tropics in historical times. while this suggests man-mediated dispersal, estimating contemporary connectivity among populations has remained elusive. here, we use a large mtdna dataset and a bayesian coalescent framework to test a set of hypotheses about gene flow among american ae. aegypti populations. we assessed gene flow patterns at the continental and subregional (amazon basin) scales. for the americas, our data ...	23144654
anopheles nf-κb-regulated splicing factors direct pathogen-specific repertoires of the hypervariable pattern recognition receptor agdscam.	insects rely on innate immune responses controlled by the immune deficiency (imd), toll, and other immune signaling pathways to combat infection by a broad spectrum of pathogens. these pathways signal to downstream nf-κb family transcription factors that control specific antipathogen action via direct transcriptional control of immune effectors, hematopoiesis, and melanization. here we show that in the anopheles malaria vector, imd and toll pathways mediate species-specific defenses against plas ...	23084919
pyrethroid resistance in aedes aegypti and aedes albopictus from port-au-prince, haiti.	in port-au-prince, haiti, the status of insecticide resistance has not recently been evaluated for aedes aegypti (l) and aedes albopictus (skuse) populations. no prophylactics exist for dengue, so prevention is only through vector control methods. an earthquake occurred in haiti on january 12, 2010, with a magnitude of 7.0 mw that devastated the area. dengue became a major concern for the humanitarian relief workers that entered the country. bottle bioassays were conducted in the field on adult ...	23181855
lessons from malaria control to help meet the rising challenge of dengue.	achievements in malaria control could inform efforts to control the increasing global burden of dengue. better methods for quantifying dengue endemicity-equivalent to parasite prevalence surveys and endemicity mapping used for malaria-would help target resources, monitor progress, and advocate for investment in dengue prevention. success in controlling malaria has been attributed to widespread implementation of interventions with proven efficacy. an improved evidence base is needed for large-sca ...	23174383
application of eco-friendly tools and eco-bio-social strategies to control dengue vectors in urban and peri-urban settings in thailand.	dengue is considered one of the most important vector-borne diseases in thailand. its incidence is increasing despite routine implementation of national dengue control programmes. this study, conducted during 2010, aimed to demonstrate an application of integrated, community-based, eco-bio-social strategies in combination with locally-produced eco-friendly vector control tools in the dengue control programme, emphasizing urban and peri-urban settings in eastern thailand.	23318236
community mobilization and household level waste management for dengue vector control in gampaha district of sri lanka; an intervention study.	waste management through community mobilization to reduce breeding places at household level could be an effective and sustainable dengue vector control strategy in areas where vector breeding takes place in small discarded water containers. the objective of this study was to assess the validity of this assumption.	23318240
introduction.		23318233
community-based control of aedes aegypti by adoption of eco-health methods in chennai city, india.	dengue is highly endemic in chennai city, south india, in spite of continuous vector control efforts. this intervention study was aimed at establishing the efficacy as well as the favouring and limiting factors relating to a community-based environmental intervention package to control the dengue vector aedes aegypti.	23318241
estimating dengue vector abundance in the wet and dry season: implications for targeted vector control in urban and peri-urban asia.	research has shown that the classical stegomyia indices (or "larval indices") of the dengue vector aedes aegypti reflect the absence or presence of the vector but do not provide accurate measures of adult mosquito density. in contrast, pupal indices as collected in pupal productivity surveys are a much better proxy indicator for adult vector abundance. however, it is unknown when it is most optimal to conduct pupal productivity surveys, in the wet or in the dry season or in both, to inform contr ...	23318235
eco-bio-social research on dengue in asia: a multicountry study on ecosystem and community-based approaches for the control of dengue vectors in urban and peri-urban asia.	this article provides an overview of methods and cross-site insights of a 5-year research and capacity building initiative conducted between 2006 and 2011 in six countries of south asia (india, sri lanka) and south-east asia (indonesia, myanmar, philippines, thailand).the initiative managed an interdisciplinary investigation of ecological, biological, and social (i.e., eco-bio-social) dimensions of dengue in urban and peri-urban areas, and developed community-based interventions aimed at reducin ...	23318234
genetically modified insects as a public health tool: discussing the different bio-objectification within genetic strategies.		23275331
promoting health and advancing development through improved housing in low-income settings.	there is major untapped potential to improve health in low-income communities through improved housing design, fittings, materials and construction. adverse effects on health from inadequate housing can occur through a range of mechanisms, both direct and indirect, including as a result of extreme weather, household air pollution, injuries or burns, the ingress of disease vectors and lack of clean water and sanitation. collaborative action between public health professionals and those involved i ...	23271143
factors associated with dengue mortality in latin america and the caribbean, 1995-2009: an ecological study.	in this study, we aimed to estimate the effect that environmental, demographic, and socioeconomic factors have on dengue mortality in latin america and the caribbean. to that end, we conducted an observational ecological study, analyzing data collected between 1995 and 2009. dengue mortality rates were highest in the caribbean (spanish-speaking and non-spanish-speaking). multivariate analysis through poisson regression revealed that the following factors were independently associated with dengue ...	22302870
papaya, dengue fever and ayurveda.		24501439
seroprevalence of chikungunya in southern odisha.	the emergence of chikungunya (chik) infection was observed in odisha, india in 2006. thereafter many cases with symptoms suggestive of chik were reported from different districts of southern-odisha. this study was aimed to know the seroprevalence, clinical presentations and seasonal trends of chik infection in this region.	24479040
effects of the botanical insecticide, toosendanin, on blood digestion and egg production by female aedes aegypti (diptera: culicidae): topical application and ingestion.	botanical insecticides offer novel chemistries and actions that may provide effective mosquito control. toosendanin (tsn, 95% purity) is one such insecticide used to control crop pests in china, and in this study, it was evaluated for lethal and sublethal effects on larvae and females of the yellowfever mosquito, aedes aegypti (l.). tsn was very toxic to first instar larvae after a 24 h exposure (lc50 = 60.8 microg/ml) and to adult females up to 96 h after topical treatment (ld50 = 4.3 microg/fe ...	23427659
roles of pkc and phospho-adducin in transepithelial fluid secretion by malpighian tubules of the yellow fever mosquito.	the diuretic hormone aedeskinin-iii is known to increase the paracellular cl(-) conductance in malpighian (renal) tubules of the mosquito aedes aegypti via a g protein-coupled receptor. the increase serves the blood-meal-initiated diuresis and is associated with elevated levels of ca(2+) and phosphorylated adducin in the cytosol of tubule. in the present study we have cloned adducin in aedes malpighian tubules and investigated its physiological roles. immunolabeling experiments are consistent wi ...	24062972
hemoperitoneum in dengue fever with normal coagulation profile.	a 43-year-old male living in bengaluru sought emergency services due to high-grade fever, headache, myalgia, abdominal pain and distension. platelet count (except the first-96,000/mm(3)) and coagulation profile was in normal limits. the dengue serology was positive for igm and ig g (immunoglobulin m and g) antibodies. ultrasound abdomen showed gross ascites, mild bilateral pleural effusion and hepatosplenomegaly. the patient continued to have abdominal pain and progressive distention ascitic tap ...	23599615
the phospholipase c isozymes and their regulation.	the physiological effects of many extracellular neurotransmitters, hormones, growth factors, and other stimuli are mediated by receptor-promoted activation of phospholipase c (plc) and consequential activation of inositol lipid signaling pathways. these signaling responses include the classically described conversion of phosphatidylinositol(4,5)p(2) to the ca(2+)-mobilizing second messenger inositol(1,4,5)p(3) and the protein kinase c-activating second messenger diacylglycerol as well as alterat ...	22403074
dengue antibodies in blood donors.	dengue is an urban arbovirus whose etiologic agent is a virus of the genus flavorius with four distinct antigen serotypes (denv-1, denv-2, denv-3 and denv-4) that is transmitted to humans through the bite of the mosquito aedes aegypti. the campo mourão region in brazil is endemic for dengue fever. obtective: the aim of this study was to evaluate the presence of igg and igm antibodies specific to the four serotypes of dengue in donors of the blood donor service in the city of campo mourão.	23049418