Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the mossie-buster: a hose-driven insecticide delivery tool for the control of container-breeding mosquitoes. | a novel insecticide delivery tool, the mossie-buster, was recently developed to control larval populations from urban breeding sites in townsville, australia. this functional and user-friendly control device directly targets the main breeding sites of aedes aegypti (l.) with a focused delivery of insecticides. the mossie-buster comprises a commercially available device and attachments that mix an insecticide solution into the flow of water emitted from a hose. attached to the device is a trigger ... | 1997 | 9474568 |
| a novel insecticidal serotype of clostridium bifermentans. | a novel clostridium bifermentans strain toxic to mosquito larvae on ingestion was isolated from a soil sample collected from secondary forest floor. this strain was designated as serovar paraiba (c. b. paraiba) according to its specific h antigen. clostridium bifermentans paraiba is most toxic to anopheles maculatus theobald larvae (lc50 = 0.038 mg/liter), whereas toxicity to aedes aegypti (linn.) (lc50 = 0.74 mg/liter) and culex quinquefasciatus say (lc50 = 0.11 mg/liter) larvae was 20 and 3 ti ... | 1997 | 9474569 |
| differential accumulation and tissue distribution of mosquito hexamerins during metamorphosis. | the pupal hexamerins were characterized for two mosquitoes representative of the culicine and anopheline families, aedes aegypti and anopheles gambiae. like higher diptera, both mosquito species express two types of hexamerins, hex-1 and hex-2, whose subunits are distinguished by different levels of methionine and aromatic amino acids. in a. aegypti there are two heterohexamers, aahex-1 and aahex-2. in a. gambiae there are two homohexamers, aghex-1.1 and aghex-1.2, and one heterohexamer, aghex-2 ... | 1997 | 9474778 |
| transamination of 3-hydroxykynurenine to produce xanthurenic acid: a major branch pathway of tryptophan metabolism in the mosquito, aedes aegypti, during larval development. | an electrochemically active compound was detected in the larvae of aedes aegypti mosquitoes and progressive accumulation of this compound was observed during larval development. the compound was purified from mosquito larvae using various chromatographic techniques and spectral analysis of the purified compound resulted in its identification as xanthurenic acid. production of xanthurenic acid results from the transamination of 3-hydroxykynuorenine, and analysis of the biochemical pathway in xant ... | 1997 | 9474782 |
| global situation of dengue and dengue haemorrhagic fever, and its emergence in the americas. | about two-thirds of the world's population live in areas infested with dengue vectors, mainly aedes aegypti. all four dengue viruses are circulating, sometimes simultaneously, in most of these areas. it is estimated that up to 80 million persons become infected annually although marked underreporting results in the notification of much smaller figures. currently dengue is endemic in all continents except europe and epidemic dengue haemorrhagic fever (dhf) occurs in asia, the americas and some pa ... | 1997 | 9477544 |
| down regulation of macrophage activation in brugia pahangi-infected jirds (meriones unguiculatus). | the macrophage is a major component of the inflammatory response induced by lymphatic tissue-dwelling filariae. intraperitoneal (i.p.) infections with brugia pahangi in mongolian gerbils, or jirds (meriones unguiculatus), induce a peritoneal inflammatory response characterized by accumulation of numerous macrophages and fewer eosinophils. this inflammatory response is associated with the release of microfilariae by female worms. the aim of this study was to investigate the activation state of th ... | 1998 | 9488396 |
| preliminary field evaluation of the combined adulticidal, larvicidal, and wall residual activity of ulv-applied bifenthrin against mosquitoes. | the combined adulticidal, larvicidal, and wall residual activity of ulv-applied bifenthrin, a synthetic pyrethroid, was evaluated in houses in kuala lumpur, malaysia, against larvae and adults of lab-bred aedes aegypti and culex quinquefasciatus. a portable ulv sprayer was used to disperse a ulv formulation of bifenthrin at a discharge rate of 45 ml/min. the results indicated that bifenthrin sprayed at this rate exhibited all the three activities against the test mosquitoes. complete adult morta ... | 1997 | 9491365 |
| dengue--a re-emerging infectious disease in singapore. | despite its well-established integrated nationwide aedes mosquito control programme which incorporates source reduction, public health education and law enforcement, singapore has not been spared from the regional resurgence of dengue. the disease incidence has been increasing from 9.3 per 100,000 in 1988 to 102.7 per 100,000 in 1996 at the time when the aedes house index (hi) has dropped to around 1% from > 25% in the 1960s. majority of the cases reported from 1990 to 1996 were dengue fever (df ... | 1997 | 9494676 |
| la untadita: a procedure for maintaining washbasins and drums free of aedes aegypti based on modification of existing practices. | chlorine bleach and detergent are routinely used by householders in el progreso, honduras in the process of cleaning washbasins and drums, the two most important larval habitats of aedes aegypti in the city. the efficacy of these materials in eliminating eggs, larvae, and pupae of ae. aegypti was assessed under controlled conditions. the promising results obtained led to trials using a combination of chlorine bleach and detergent to apply to the walls of washbasins and drums as a method for elim ... | 1998 | 9502612 |
| evidence for genetic hitchhiking effect associated with insecticide resistance in aedes aegypti. | information on genetic variation within and between populations is critical for understanding the evolutionary history of mosquito populations and disease epidemiology. previous studies with drosophila suggest that genetic variation of selectively neutral loci in a large fraction of genome may be constrained by fixation of advantageous mutations associated with hitchhiking effect. this study examined restriction fragment length polymorphisms of four natural aedes aegypti mosquito populations fro ... | 1998 | 9504925 |
| hemagglutinins in mosquitoes and their role in the immune response to brugia malayi (filarioidea:nematoda) larvae. | hemagglutinins were determined in six species of mosquitoes that are susceptible and refractory to brugia malayi (filarioidea: nematoda). high titers of hemagglutinins were found in the salivary gland extract and in the body fluid of a completely refractory species, aedes taeniorhynchus, and in partially refractory species, anopheles quadrimculatus but low levels of hemagglutinins were also present in the body fluid of aedes aegypti (black-eye, liverpool strain), a susceptible species. hemagglut ... | 1997 | 9505436 |
| [atypical activity rhythm in aedes aegypti in a sub-sudanian savannah zone of côte d'ivoire]. | ae. aegypti is vector of many arboviruses of medical and/or economical importance. it transmits the yellow fever, dengue and chikungunya viruses. small water collections constitute its potential breeding sites. three different populations of ae. aegypti are known in africa: a selvatic population breeding in tree holes and rock holes, a population breeding in discarded containers in peri-domestic area and a third population which breeds in drinking water containers and various receptacles inside ... | 1997 | 9507772 |
| mosquito density, biting rate and cage size effects on repellent tests. | mosquito biting rates and the mean duration of protection (in hours) from bites (mdpb) of aedes aegypti and anopheles quadrimaculatus, using the repellent 'deet' (n,n-diethyl-3-methylbenzamide) on a 50 cm2 area of healthy human skin, were observed in small (27 l), medium (approximately 65 l) and large (125 l) cages containing low, medium or high densities of mosquitoes: respectively, 640, 128 or 49 cm3 of cage volume per female. at the initial treatment rate of approximately 0.4 microliter/cm2 ( ... | 1998 | 9513937 |
| effect of heavy metals on aedes aegypti (diptera: culicidae) larvae. | studies were conducted to determine the biological effects of heavy metals on the development of aedes aegypti. embryos immersed in 32 ppm cu or 5 ppm cd did not hatch. the arrest of hatching was in part reversible by removal of the heavy metals. the mortality rate of third-instar larvae exposed to heavy metals for 24 h was metal and dose dependent; the 50% lethal concentration (lc50) endpoints were 3.1, 16.5, and 33 ppm for hg, cd, and cu, respectively. interestingly, a proportion of aedes aegy ... | 1998 | 9515074 |
| survey of cyclopids (crustacea, copepoda) in brazil and preliminary screening of their potential as dengue vector predators. | cyclopid copepods are known to be good mosquito controllers, specially as regards the larvae of the dengue vectors aedes aegypti and ae. albopictus. | 1997 | 9515258 |
| an important step forward in the genetic manipulation of mosquito vectors of human disease. | 1998 | 9520367 | |
| plasmodium gallinaceum preferentially invades vesicular atpase-expressing cells in aedes aegypti midgut. | penetration of the mosquito midgut epithelium is obligatory for the further development of plasmodium parasites. therefore, blocking the parasite from invading the midgut wall disrupts the transmission of malaria. despite such a pivotal role in malaria transmission, the cellular and molecular interactions that occur during the invasion are not understood. here, we demonstrate that the ookinetes of plasmodium gallinaceum, which is related closely to the human malaria parasite plasmodium falciparu ... | 1998 | 9520375 |
| stable transformation of the yellow fever mosquito, aedes aegypti, with the hermes element from the housefly. | the mosquito aedes aegypti is the world's most important vector of yellow fever and dengue viruses. work is currently in progress to control the transmission of these viruses by genetically altering the capacity of wild ae. aegypti populations to support virus replication. the germ-line transformation system reported here constitutes a major advance toward the implementation of this control strategy. a modified hermes transposon carrying a 4.7-kb fragment of genomic dna that includes a wild-type ... | 1998 | 9520437 |
| mariner transposition and transformation of the yellow fever mosquito, aedes aegypti. | the mariner transposable element is capable of interplasmid transposition in the embryonic soma of the yellow fever mosquito, aedes aegypti. to determine if this demonstrated mobility could be utilized to genetically transform the mosquito, a modified mariner element marked with a wild-type allele of the drosophila melanogaster cinnabar gene was microinjected into embryos of a kynurenine hydroxylase-deficient, white-eyed recipient strain. three of 69 fertile male founders resulting from the micr ... | 0 | 9520438 |
| development of a chimeric sindbis virus with enhanced per os infection of aedes aegypti. | the te/3'2j double subgenomic sindbis (dssin) viruses have been used to stably express genes in aedes aegypti nerve and salivary gland tissues. however, because these viruses inefficiently infect ae. aegypti when administered by the per os route, te/3'2j viruses must be intrathoracically inoculated into the mosquitoes to infect these tissues. a malaysian sindbis (sin) virus isolate (mre16) does efficiently infect ae. aegypti midgut tissues after ingestion, and approximately 95% of these mosquito ... | 1998 | 9527919 |
| putative chikungunya virus-specific receptor proteins on the midgut brush border membrane of aedes aegypti mosquito. | two proteins (putative receptors) of 60 and 38 kda, for chikungunya (chik) virus were detected in the brush border membrane fraction (bbmf) of the normal population of aedes aegypti mosquitoes. mosquitoes were infected orally with chik virus and infectivity checked by testing the head squashes. bbmf was prepared from proved positive and negative mosquitoes. the receptor proteins were found to be present in both the proved genotypes. however, dot-b'ot assays showed that the chik virus binding act ... | 1998 | 9529775 |
| expression and rapid purification of an aedes aegypti salivary allergen by a baculovirus system. | mosquito salivary proteins cause allergic reactions in humans. recombinant salivary allergens will facilitate both the diagnosis and immunotherapy of mosquito allergy. the aed a 1, a 68-kd apyrase in the saliva of aedes aegypti, has been demonstrated to be an allergen which binds to the ige of mosquito-allergic subjects. the baculovirus expression vector pbluebachis c equipped with an n-terminal histidine tag was used to express the aed a 1 protein. the cdna coding for the 3' significant portion ... | 1998 | 9531167 |
| phylogeny of amblyospora (microsporida: amblyosporidae) and related genera based on small subunit ribosomal dna data: a possible example of host parasite cospeciation. | small subunit ribosomal rna (ssu rrna) gene sequences were analyzed for six species and four genera of microsporidia from mosquito hosts; amblyospora stimuli (aedes stimulans), amblyospora californica (culex tarsalis), amblyospora sp. (culex salinarius), edhazardia aedis (aedes aegypti), culicosporella lunata (culex pilosus), and parathelohania anophelis (anopheles quadrimaculatus). comparison of these sequences to those of other microsporidia show that these sequences are longer with the ssu rr ... | 1998 | 9538024 |
| serum composition of aedes aegypti (diptera: culicidae) larvae and the production of an oviposition repellent are influenced by infection with the entomopathogenic digenean plagiorchis elegans (trematoda: plagiorchiidae), starvation, and crowding. | subjecting aedes aegypti (l.) larvae to conditions that induced the production of oviposition repellency also reduced their wet and dry weights and the concentration of total serum carbohydrates, amino acids, and proteins. thus, infection with metacercariae of the entomopathogenic digenean plagiorchis elegans (rudolphi), starvation for 7 d, or crowding for 2 d reduced larval dry weights by as much as 32, 20, and 23%, respectively, and wet weights by 20, 14, and 11%, respectively. total serum car ... | 1998 | 9538578 |
| two contiguous outbreaks of dengue type 2 in north queensland. | to investigate two outbreaks of dengue type 2 in north queensland, one in the torres strait beginning in late 1996, the other in a cairns suburb in early 1997. | 1998 | 9539900 |
| antifungal and larvicidal meroterpenoid naphthoquinones and a naphthoxirene from the roots of cordia linnaei. | three new meroterpenoid naphthoquinones, the known cordiaquinone b and a new naphthoxirene have been isolated from the roots of cordia linnaei. their structures were established by spectrometric methods including ei, d/ci and fab mass spectrometry, 1h, 13c and 2d nmr experiments. the naphthoquinones showed activity against cladosporium cucumerinum, candida albicans and the larvae of the yellow fever-transmitting mosquito aedes aegypti, while the naphthoxirene derivative was found to be inactive ... | 1998 | 9542168 |
| ultrastructural studies on the reproductive system of male aedes aegypti (diptera: culicidae) infected with dengue 2 virus. | dengue 2 virus was found by transmission electron microscopy to be present in the reproductive tissues of male aedes aegypti (l) 14 d after intrathoracic inoculation. dengue 2 particles were detected in the matrix, epithelial cells, and the peripheral fat body of the testes; secretory droplets of columnar cells of the accessory glands; and the epithelial and muscle cells of the seminal vesicles. however, none was found in the germ cells (i.e., spermatogonia, spermatocytes, spermatid, or spermato ... | 1998 | 9542348 |
| aedes aegypti survival and dispersal estimated by mark-release-recapture in northern australia. | the survival and dispersal of adult aedes aegypti were estimated in northern australia where sporadic outbreaks of ae. aegypti-borne dengue viruses have occurred in recent years. standard mark-release-recapture methods were used. in addition, a new sticky trap was used to capture the mosquitoes. prior to the field study, the survival and effect of marking ae. aegypti with fluorescent powder were determined in the laboratory. mortality was age-dependent and the marked cohorts had higher survival ... | 1998 | 9546403 |
| effect of size and geographic origin of aedes aegypti on oral infection with dengue-2 virus. | differences in larval habitats cause variation in the size of aedes aegypti (l.) adults. we suspected that such size variation was related to the ease with which the mosquitoes could be infected with dengue virus. using a rearing procedure that produced three distinct size classes of mosquitoes, we determined the percentage of mosquitoes that developed disseminated dengue-2 infection following oral feeding with a suspension containing 3.3 x 10(7) plaque-forming units/ml. mosquitoes were reared f ... | 1998 | 9546404 |
| reduced transdermal absorption of n,n-diethyl-m-toluamide from a new topical insect repellent formulation. | extensive absorption of the topical insect repellent n, n-diethyl-m-toluamide (deet) causes systemic and local toxicities. this report describes the preparation and characterization of a new insect repellent formulation (fa), a peg-polyacrylic acid polymer system, for its deet release, in vitro skin permeation, and in vivo transdermal absorption properties; and for its relative repellency against aedes aegypti mosquitoes. deet release and skin permeation were studied in franz diffusion cells. de ... | 1997 | 9552429 |
| gene expression and regulation from the p7 promoter of aedes densonucleosis virus. | the nonstructural proteins ns1 and ns2 are thought to be expressed from the p7 promoter of aedes densonucleosis virus (aednv). to study gene expression from the p7 promoter, eight different plasmids were constructed by fusing beta-galactosidase or beta-glucuronidase into the genome so that the reporter gene was in different open reading frames and under the transcriptional control of the p7 promoter. after transfection into c6/36 aedes albopictus cells, constructs generated comparable amounts of ... | 1998 | 9557726 |
| [dengue, arbovirus and migrations in the indian ocean]. | an arbovirus requires a competent haematophagous arthropod for transmission from one vertebrate to another susceptible host. air transportations allow quick transfers from place to place and the diffusion of potential vectors or infectious hosts. the merchandise transportation by containers allowed the diffusion of ae. albopictus from asia through africa and america. emerging arbovirusis may occur in zones where they do not exist. dengue which originally was located in southeastern asia, is the ... | 1998 | 9559164 |
| effect of sublethal dosages of malathion on the oral susceptibility of aedes aegypti to dengue-2 virus infection. | 1997 | 9561626 | |
| plasmodium gallinaceum: effect of insect cells on ookinete development in vitro. | in vitro culture conditions affecting the transformation efficiency from zygote to ookinete for plasmodium gallinaceum were examined, as a step toward improving the overall efficiency of in vitro culture systems for sporogonic stages. gametocytes from infected chickens were allowed to fertilize in vitro and the resulting zygotes were purified and cultured. the time course for ookinete development in vitro was similar to that seen in aedes aegypti mosquitoes. supplementing a basal m-199 culture m ... | 1998 | 9562424 |
| transient expression of the drosophila melanogaster cinnabar gene rescues eye color in the white eye (we) strain of aedes aegypti. | the lack of eye pigment in the aedes aegypti we (white eye) colony was confirmed to be due to a mutation in the kynurenine hydroxylase gene, which catalyzes one of the steps in the metabolic synthesis of ommochrome eye pigments. partial restoration of eye color (orange to red phenotype) in pupae and adults occurred in both sexes when first or second instar larvae were reared in water containing 3-hydroxykynurenine, the metabolic product of the enzyme kynurenine hydroxylase. no eye color restorat ... | 1997 | 9569641 |
| cloning of cdna for vitellogenin of the parasitoid wasp, pimpla nipponica (hymenoptera: apocrita: ichneumonidae): vitellogenin primary structure and evolutionary considerations. | the cdna for vitellogenin (vg) of the parasitoid wasp pimpla nipponica (hymenoptera: apocrita) was cloned and sequenced. the deduced amino acid sequence with 1807 residues was obtained. the n-terminal 20 amino acids chemically determined for vitellin (vn) agreed completely with the deduced 20 amino acids that follow the 16 amino acid residues for putative signal peptide. the cdna clone for the vg of the turnip sawfly athalia rosae (hymenoptera: symphyta), previously obtained and partially sequen ... | 1997 | 9569645 |
| germination, growth, and sporulation of bacillus thuringiensis subsp. israelensis in excreted food vacuoles of the protozoan tetrahymena pyriformis. | spores of bacillus thuringiensis subsp. israelensis and their toxic crystals are bioencapsulated in the protozoan tetrahymena pyriformis, in which the toxin remains stable. each t. pyriformis cell concentrates the spores and crystals in its food vacuoles, thus delivering them to mosquito larvae, which rapidly die. vacuoles containing undigested material are later excreted from the cells. the fate of spores and toxin inside the food vacuoles was determined at various times after excretion by phas ... | 1998 | 9572947 |
| involvement of superoxide dismutase in spore coat assembly in bacillus subtilis. | endospores of bacillus subtilis are enclosed in a proteinaceous coat which can be differentiated into a thick, striated outer layer and a thinner, lamellar inner layer. we found that the n-terminal sequence of a 25-kda protein present in a preparation of spore coat proteins matched that of the mn-dependent superoxide dismutase (sod) encoded by the sod4 locus. sod4 is transcribed throughout the growth and sporulation of a wild-type strain and is responsible for the sod activity detected in total ... | 1998 | 9573176 |
| the concentration-dependence of crf-like diuretic peptide: mechanisms of action. | the mechanism of action of synthetic ccrf-dp, the corticotropin-releasing factor (crf)-related diuretic peptide of the salt marsh mosquito culex salinarius, was investigated in isolated malpighian tubules of the yellow fever mosquito aedes aegypti. a low concentration of ccrf-dp (10(-9)mol l-1) caused a small but insignificant increase in transepithelial secretion of nacl and fluid, but significantly reduced transepithelial voltage and resistance without a change in short-circuit current, pointi ... | 1998 | 9576886 |
| the ultraspiracle gene of the spruce budworm, choristoneura fumiferana: cloning of cdna and developmental expression of mrna. | cloning and characterization of a choristoneura fumiferana ultraspiracle (cfusp) cdna are described. first, a pcr fragment and then a cdna clone (4.4 kb) were isolated from spruce budworm cdna libraries. comparison of the deduced amino acid sequence of this cdna with the sequences in genbank showed that this sequence had high homology with the ultraspiracle cdnas cloned from drosophila melanogaster (dmusp), bombyx mori (bmusp), manduca sexta (msusp), and aedes aegypti (aausp). the cfusp cdna con ... | 1998 | 9581288 |
| adherence of erythrocytes during exflagellation of plasmodium falciparum microgametes is dependent on erythrocyte surface sialic acid and glycophorins. | malaria male gametocytes within a newly ingested infected blood meal in the mosquito midgut emerge from erythrocytes and extrude approximately eight flagellar microgametes in a process termed exflagellation. in culture, and in blood removed from infected patients, emerging microgametes avidly adhere to neighboring uninfected and infected erythrocytes, as well as to emerged female macrogametes, creating "exflagellation centers". the mechanism of erythrocyte adherence is not known nor has it been ... | 1998 | 9584138 |
| effects on larvicidal activity of single proline substitutions in alpha3 or alpha4 of the bacillus thuringiensis cry4b toxin. | the possible role of alpha-helices 3 and 4 in toxicity of the dipteran-active bacillus thuringiensis cry4b delta-endotoxin was investigated by employing proline substitutions via site-directed mutagenesis. similar to the wild-type cry4b, the mutant toxins were over-expressed in escherichia coli as cytoplasmic inclusions and were structurally stable upon solubilization and trypsin activation. the substitution of glutamine 149 by proline in the center of helix 4 (q149p) resulted in a nearly comple ... | 1998 | 9584996 |
| monitoring of dengue viruses in field-caught aedes aegypti and aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing. | virologic surveillance for dengue through the detection of the prevalent serotype(s) circulating in the human population during inter- and intra-epidemic periods constitutes a reliable sentinel system for dengue outbreaks. we have applied a rapid and sensitive, semi-nested, reverse transcription-polymerase chain reaction (rt-pcr) assay using nonstructural protein 3 gene primers for the type-specific-detection of dengue viruses in artificially infected and in field-caught adult aedes mosquitoes. ... | 1998 | 9598444 |
| engineered resistance in aedes aegypti to a west african and a south american strain of yellow fever virus. | double subgenomic sindbis (dssin) viruses were engineered to transduce mosquito cells with antisense rna derived either from the premembrane (prm) or polymerase (ns5) coding regions of the 17d vaccine strain of yellow fever virus (yfv). aedes albopictus c6/36 cells were infected at high multiplicities of infection (moi) with each dssin virus. forty-eight hours later, the transduced cells were challenged with an moi of 0.1 of the asibi strain of yfv. at 72-hr postchallenge, the cells were assayed ... | 1998 | 9598458 |
| natural habitats of aedes aegypti in the caribbean--a review. | natural breeding habitats of aedes aegypti in the caribbean region were reviewed by conducting larval surveys in trinidad. puerto rico, and the u.s. virgin islands and referring to records from the mosquitoes of middle america project. twelve types of natural habitats were recorded: rock holes (9.7%), calabashes (2.4%), tree holes (19.5%), leaf axils (4.8%), bamboo joints (14.9%), papaya stumps (7.3%), coconut shells (4.8%), bromeliads (7.3%), ground pools (14.9%), coral rock holes (9.7%), crab ... | 1998 | 9599318 |
| laboratory bioassay to compare susceptibilities of aedes aegypti and anopheles albimanus to bacillus thuringiensis var. israelensis as affected by their feeding rates. | this study presents the effect of differences in the feeding rates of aedes aegypti and anopheles albimanus on their susceptibilities to bacillus thuringiensis israelensis. aedes aegypti was more susceptible than an. albimanus because of its faster rate of feeding. aedes aegypti ingested 11.5 times more spores than did an. albimanus, resulting in lower lt50 values. anopheles albimanus larvae fed at a slower rate and required fewer spores than ae. aegypti to induce 50% mortality. these findings s ... | 1998 | 9599326 |
| the lethal effects of cyperus iria on aedes aegypti. | the sedge cyperus iria, a common weed in rice, contains large amounts of the insect hormone (10r) juvenile hormone iii (jh iii). given its widespread distribution in asia and africa, we examined the possibility that c. iria could be used as a safe, inexpensive, and readily available mosquito larvicide. plants of varying ages were harvested and leaves tested for lethal effects on larvae of the yellow fever mosquito, aedes aegypti. the median lethal doses (ld50s) for frozen leaves from 1- and 2-mo ... | 1998 | 9599328 |
| nucleotide sequence and deduced amino acid sequence of a putative asparagine synthetase in the mosquito aedes aegypti (l.). | a cdna was cloned from a aedes aegypti head cdna library, containing the complete coding sequence for an asparagine synthetase homolog. the predicted polypeptide sequence exhibits high homology with different proteins of the 'purf' glutamine amidotransferase enzyme family. the aminoterminal region, containing cys-1 which is crucial to perform the glutaminase reaction, was highly conserved among the asparagine synthetase family. subsequent expression of the cdna yielded a 54,000 da protein corres ... | 1998 | 9602118 |
| plasmodium gallinaceum: differential killing of some mosquito stages of the parasite by insect defensin. | we examined several insect antimicrobial peptides to study their effect on plasmodium gallinaceum zygotes, ookinetes, oocysts, and sporozoites. only two insect defensins-aeschna cyanea (dragon fly) and phormia terranovae (flesh fly)-had a profound toxic effect on the oocysts in aedes aegypti and on isolated sporozoites. the defensins affected the oocysts in a time-dependent manner. injecting the peptide into the hemolymph 1 or 2 days after an infectious blood meal had no significant effect on pr ... | 1998 | 9603495 |
| clinical evaluation of a rapid immunochromatographic test for the diagnosis of dengue virus infection. | a rapid immunochromatographic test was compared to the hemagglutination inhibition assay for separate determinations of dengue virus-specific immunoglobulin m (igm) and igg levels in paired serum specimens from 92 patients (34 with primary dengue virus infection, 35 with secondary dengue virus infection, and 23 without dengue virus infection). the rapid test showed 99% sensitivity in the diagnosis of dengue virus infection. the majority (30 of 34 [88%]) of patients with primary infection showed ... | 1998 | 9606000 |
| dose-dependent effects of crf-like diuretic peptide on transcellular and paracellular transport pathways. | the mechanism of action of synthetic culex corticotropin-releasing factor (crf)-like diuretic peptide (ccrf-dp) was investigated in isolated, perfused malpighian tubules of the yellow fever mosquito, aedes aegypti. low concentrations of ccrf-dp (10(-10) and 10(-9) m) caused depolarizing oscillations of the lumen-positive transepithelial voltage (vt) in malpighian tubules, whereas high concentrations (10(-8) and 10(-7) m) first depolarized and then transiently hyperpolarized vt; ccrf-dp always lo ... | 1998 | 9612320 |
| carboxypeptidase b in anopheles gambiae (diptera: culicidae): effects of abdominal distention and blood ingestion. | carboxypeptidase b (cpb) activity was detected in the guts of strain g-3 of anopheles gambiae (giles) and aedes aegypti (l.). mosquitoes were examined 3-5 d after emergence following exposure to 20% sucrose, from 0 to 4 h after feeding on a meal of latex beads in saline, and from 0 to 96 h after blood feeding. cpb activity was assayed in whole-gut homogenates, including lumenal contents and peritrophic matrix, by following the hydrolysis of a substrate specific for cpb-[3h]-benzoyl-l-phe-l-arg. ... | 1998 | 9615537 |
| mediation of deet repellency in mosquitoes (diptera: culicidae) by species, age, and parity. | laboratory bioassays assessed differences in the protection time provided by the repellent deet (n,n-diethyl-3-methylbenzamide) against 5-d-old nulliparous and 10-, 15-, and 20-d-old nulliparous and parous female aedes aegypti (l.), anopheles albimanus (weidemann), and anopheles quadrimaculatus say sensu lato. mean protection time was shortest against an. albimanus (1.6 h) and an. quadrimaculatus (1.5 h) and longest against ae. aegypti (6.5 h), but was not significantly influenced by mosquito ag ... | 1998 | 9615557 |
| the lepidopteran transposon vector, piggybac, mediates germ-line transformation in the mediterranean fruit fly. | the piggybac (ifp2) short inverted terminal repeat transposable element from the cabbage looper trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector-helper system in the mediterranean fruit fly ceratitis capitata. a piggybac vector marked with the medfly white gene was tested with a normally regulated piggybac transposase helper at two different concentrations in a white eye host strain. both experiments yielded transformants at an approximate frequency of 3 ... | 0 | 9636182 |
| cytotoxic and insecticidal constituents of the unripe fruit of persea americana. | unripe avocado fruit (persea americana) was subjected to a bioactivity-directed fractionation, as monitored via the brine shrimp lethality assay, to isolate three major bioactive constituents (1-3). compounds 1-3 all have a 1,2,4-triol moiety and a long aliphatic chain that terminates as either an alkane, alkene, or alkyne, respectively; 1 is new, while 2 and 3 have been described previously but not as cytotoxic or pesticidal agents. all have activity against six human tumor cell lines in cultur ... | 1998 | 9644064 |
| detection of japanese encephalitis virus by reverse transcription/polymerase chain reaction. | a simple and rapid reverse transcription/polymerase chain reaction (rt-pcr) assay for detection of japanese encephalitis virus (jev) envelope (e) gene sequences in various biological samples is described. the assay successfully amplified jev e gene sequences from infected cell cultures, aedes aegypti larvae, mosquitoes and mouse blood. the sensitivity of the assay was currently 1 ng of jev rna and could be increased up to 1 pg on the background of 1 microgram of cellular rna by biotinylation of ... | 1998 | 9645237 |
| insecticidal activity of bacillus laterosporus. | the bacillus laterosporus strains 921 and 615 were shown to have toxicity for larvae of the mosquitoes aedes aegypti, anopheles stephensi, and culex pipiens. the larvicidal activity of b. laterosporus was associated with spores and crystalline inclusions. purified b. laterosporus 615 crystals were highly toxic for aedes aegypti and anopheles stephensi. | 1998 | 9647857 |
| high level transactivation by a modified bombyx ecdysone receptor in mammalian cells without exogenous retinoid x receptor. | our studies of the bombyx mori ecdysone receptor (be) revealed that, unlike the drosophila melanogaster ecdysone receptor (de), treatment of be with the ecdysone agonist tebufenozide stimulated high level transactivation in mammalian cells without adding an exogenous heterodimer partner. gel mobility shift and transfection assays with both the ultraspiracle gene product (usp) and retinoid x receptor heterodimer partners indicated that this property of be stems from significantly augmented hetero ... | 0 | 9653129 |
| identification and cdna cloning of novel juvenile hormone responsive genes from fat body of the bean bug, riptortus clavatus by mrna differential display. | juvenile hormone (jh) induces termination of diapause and initiation of reproductive maturation in the adult female bean bug, riportus clavatus. applying pcr-based differential display, we have identified four novel jh-responsive cdnas, that is, three repressible (jr-1, 2 and 3) and one inducible (ji-1). these dna fragments were partially sequenced and compared with sequences in the data base. jr-3 was shown to have similarity to the transferrins of other insects, which have been reported as jh- ... | 1998 | 9654740 |
| breeding habitats of aedes aegypti (l) and aedes. albopictus (skuse) in villages of barru, south sulawesi, indonesia. | the breeding habitats of the dengue vector, aedes aegypti and aedes albopictus, were studied using larval collection method inside and outside houses in 6 villages of barru, south sulawesi, indonesia from july 1994 to august 1995. aedes aegypti was the dominant species, being abundant indoors especially in the coastal areas. aedes albopictus was breeding primarily in outdoor containers in the hill and mountain areas. earthen jar was the most common breeding habitat of aedes aegypti in all villag ... | 1997 | 9656413 |
| laboratory evaluation of the biocontrol potential of mesocyclops thermocyclopoides (copepoda: cyclopidae) against mosquito larvae. | biocontrol potential of mesocyclops thermocyclopoides against first instar larvae of anopheles stephensi, aedes aegypti and culex quinquefasciatus was studied under laboratory conditions. it was found that m. thermocyclopoides had the highest predation efficacy against ae. aegypti followed by an. stephensi and cx. quinquefasciatus. there was a significant reduction in the predation efficacy of m. thermocyclopoides against cx. quinquefasciatus in the presence of alternate food (p < 0.01). the cag ... | 1997 | 9656415 |
| structural, genomic, and phylogenetic analysis of lian, a novel family of non-ltr retrotransposons in the yellow fever mosquito, aedes aegypti. | a retrotransposon named lian-aa1 was discovered in an intron of an aahr3-1 gene of the yellow fever mosquito, aedes aegypti. this retrotransposon contained a long open reading frame with 1,219 amino acids that included endonuclease, reverse transcriptase, and rnase h domains. it was shown that in the rock strain of ae. aegypti, there were up to 1,380 copies of lian elements, equivalent to 0.8% of the entire genome. five additional copies of lian elements were isolated, mapped by restriction dige ... | 1998 | 9656485 |
| isolation and sequence of a partial vitellogenin cdna from the cockroach, blattella germanica (l.) (dictyoptera, blattellidae), and characterization of the vitellogenin gene expression. | a partial cdna clone of the vitellogenin gene from the cockroach blattella germanica has been isolated from a cdna expression library using an anti-vitellin-vitellogenin antiserum probe. the analysis of cdna inserts gave a sequence of 2,645 nucleotides corresponding to the 3' region. the deduced amino acid sequence is 825 residues long and is similar to the homologous portion of the vitellogenin of other insect species, especially that of the mosquito aedes aegypti. rna hybridization studies ind ... | 1998 | 9658559 |
| identification of immune-related protein kinases from mosquitoes (aedes aegypti). | protein kinases are known to be involved in signal transduction for numerous physiological events. however, little is known about the roles of protein kinases in insect immunity. a fragment around 150 bp was amplified by polymerase chain reaction using cdna templates from bacterial inoculated mosquitoes and primers corresponding to the conserved domain of protein kinases. based on sequence analysis, 11 groups of protein kinases were characterized including 3 nonreceptor tyrosine kinases, 3 recep ... | 1998 | 9662071 |
| chitinases are a multi-gene family in aedes, anopheles and drosophila. | degenerate primers were used to amplify by the polymerase chain reaction (pcr) dna fragments from the chitinase genes of five insect species: aedes aegypti, anopheles freeborni, anopheles gambiae, anopheles stephensi and drosophila melanogaster. as many as four different products were found for each species; each deduced protein sequence having greatest homology to chitinase sequences from other species of insects and the crustacean, penaeus japonicus. the four pcr products of a. aegypti hybridi ... | 1998 | 9662472 |
| dengue and dengue hemorrhagic fever. | dengue fever, a very old disease, has reemerged in the past 20 years with an expanded geographic distribution of both the viruses and the mosquito vectors, increased epidemic activity, the development of hyperendemicity (the cocirculation of multiple serotypes), and the emergence of dengue hemorrhagic fever in new geographic regions. in 1998 this mosquito-borne disease is the most important tropical infectious disease after malaria, with an estimated 100 million cases of dengue fever, 500,000 ca ... | 1998 | 9665979 |
| a comparison of surveillance systems for the dengue vector aedes aegypti in port of spain, trinidad. | when the currently used larval surveillance system (visual inspection) for the dengue vector aedes aegypti was compared with the surveillance for the presence of eggs by ovitrapping in port of spain, trinidad, it was found that the latter (39.1%) was significantly more sensitive than the visual inspection system (10.1%). at the same time, the presence of the nuisance mosquito culex quinquefasciatus was detected in 38.4% of the households. both ae. aegypti and cx. quinquefasciatus showed preferen ... | 1998 | 9673912 |
| effect of female size on fecundity and survivorship of aedes aegypti fed only human blood versus human blood plus sugar. | we determined whether size, an indirect measure of teneral energy reserves, modifies the fitness advantage (sigma survival x egg production/female/day) conferred to female aedes aegypti (l.) maintained on human blood over cohorts fed human blood plus sugar. different sized females were obtained by rearing them at different larval densities and with different amounts of food per larva. each female in 4 treatment groups of 23 mosquitoes each was maintained in a separate cage. a 10% sucrose solutio ... | 1998 | 9673914 |
| north carolina mosquito records. i. uncommon aedes and anopheles (diptera: culicidae). | new distribution records are provided for 10 mosquito species that are rare or uncommon in north carolina: aedes aegypti, ae. cinereus, ae. dupreei, ae. fulvus pallens, ae. hendersoni, ae. mitchellae, ae. thibaulti, ae. tormentor, ae. trivittatus, and anopheles atropos. biological notes are provided for habitats, behavior, and, in some cases, color patterns. comments are also made about 6 additional species that are rare or uncommon in north carolina. | 1998 | 9673917 |
| effect of skin abrasions on the efficacy of the repellent deet against aedes aegypti. | abrasion of repellent-treated human skin affected the efficacy of a sustained-release insect repellent containing n,n-diethyl-3-methylbenzamide (deet) against bites of aedes aegypti. skin treated with repellent when abraded up to 30 times showed significantly lower protection than unabraded skin against mosquito bites for 10 h. the mean value of the kinetic coefficient of friction during skin abrasion by clothing (battle dress uniform fabric) for repellent-treated skin (0.159 +/- 0.003) was sign ... | 1998 | 9673919 |
| toxicity of a phenyl pyrazole insecticide, fipronil, to mosquito and chironomid midge larvae in the laboratory. | toxicity of a phenyl pyrazole insecticide, fipronil, to 4th-instar larvae of 6 species of colonized mosquitoes (aedes aegypti, ae. albopictus, ae. taeniorhynchus, anopheles quadrimaculatus, culex nigripalpus, and cx. quinquefasciatus) and 2 species of field-collected chironomid midges (chironomus crassicaudatus and glyptotendipes paripes) was evaluated in the laboratory. all mosquito species were highly susceptible with 48-h median lethal concentration (lc50) values ranging from 0.00043 ppm (ae. ... | 1998 | 9673927 |
| field evaluation of cyfluthrin and malathion 96 tg ulv spraying at high-rise flats on dengue vectors in malaysia. | cyfluthrin (solfac ulo15) and malathion 96 tg were evaluated against sentinel sugar-fed adults and 4th-instar larvae of aedes aegypti at high-rise flats in malaysia by ulv spraying. the impact of both insecticides on field populations of aedes spp. (ae. aegypti and ae. albopictus) larvae were monitored weekly using containers. both cyfluthrin and malathion 96 tg showed adulticidal effects but cyfluthrin showed more significant larvicidal effect than malathion 96 tg (p < 0.05). | 1998 | 9673932 |
| re-emergence of yellow fever in senegal in 1995. | an outbreak of yellow fever (yf) occurred in the central part of senegal during october 1995. thirty-one probable cases were detected and 79 cases were confirmed either by igm elisa or by virus isolation (30 strains isolated). the case fatality rate was 18.9%. incidence of the infection was evaluated by a serosurvey in the area. males 10-29 years old belonging to the peul ethnic group were more affected. moreover, 28 yf virus strains were isolated from mosquitoes and larvae pools and vertical tr ... | 1998 | 9684637 |
| [dengue in panama, 1993]. | up to 1993, panama was the only country in central america where the autochthonous transmission of dengue virus had been detected without experimenting an explosive epidemic despite being reinfected with the aedes aegypti mosquito since 1985. the characteristics of this first outbreak reported on november 19, 1993, are described in this paper. it is shown that even when there is a program for the surveillance and control of dengue, which considers low levels of aedes aegypti infection and a syst ... | 1997 | 9685969 |
| effectiveness of ammonium solution in relieving type i mosquito bite symptoms: a double-blind, placebo-controlled study. | a randomized, double-blind, placebo-controlled study was conducted to compare the efficacy of ammonium solution with placebo in relieving type i symptoms caused by aedes aegypti mosquitoes on 25 healthy subjects. each subject was bitten once under laboratory conditions by aedes aegypti mosquitoes on the volar aspect of each forearm, and then received the treatment with ammonium solution and placebo, respectively. compared with placebo, the ammonium solution significantly (p < 0.0001) decreased i ... | 1998 | 9689301 |
| a mouse model of mosquito allergy for study of antigen-specific ige and igg subclass responses, lymphocyte proliferation, and il-4 and ifn-gamma production. | to date, there are no ideal animal models for study of natural sensitization leading to ige- and lymphocyte-mediated hypersensitivities. we established such a model in which four balb/c mice were each sensitized by exposure to at least 16 mosquito aedes aegypti bites, twice a week for 4 weeks. four non-exposed control mice were also studied. mosquito a. aegypti head and thorax extract, saliva, and two recombinant salivary allergens (raed a 1 and raed a 2) were used in vitro as antigens. intrader ... | 1998 | 9693276 |
| isolation and characterization of the gene encoding a novel factor xa-directed anticoagulant from the yellow fever mosquito, aedes aegypti. | mosquito salivary glands secrete a number of proteins that inhibit mammalian hemostasis and facilitate blood feeding. we have isolated the protein product and corresponding cdna of a gene designated anticoagulant-factor xa (afxa), that encodes the factor xa (fxa)-directed anticoagulant of the yellow fever mosquito, aedes aegypti. the protein was purified partially by cation exchange chromatography and shown by enzyme activity profiles and sds-polyacrylamide gel electrophoresis analysis to have a ... | 1998 | 9694825 |
| coevolutionary interactions between host life histories and parasite life cycles. | several recent studies have discussed the interaction of host life-history traits and parasite life cycles. it has been observed that the life-history of a host often changes after infection by a parasite. in some cases, changes of host life-history traits reduce the costs of parasitism and can be interpreted as a form of resistance against the parasite. in other cases, changes of host life-history traits increase the parasite's transmission and can be interpreted as manipulation by the parasite ... | 1998 | 9695109 |
| trial of a community-based intervention to decrease infestation of aedes aegypti mosquitoes in cement washbasins in el progreso, honduras. | washbasins and metal drums are important sources of aedes aegypti mosquitoes in much of latin america. when manual cleaning was found to be ineffective in eliminating mosquito larvae in a community-based control programme in el progreso, honduras, it was decided to develop and evaluate an improved method of removing mosquito eggs based on commonly-available materials. the method, named la untadita ('the little dab', in english), consists of five steps: mixing chlorine bleach and detergent to mak ... | 1998 | 9698263 |
| dengue control in ribeirão preto, são paulo, brazil. | following an epidemic of type i dengue in late 1990, the municipality of ribeirão preto (state of são paulo, brazil) assumed direct responsibility for the control of aedes aegypti larvae. control activities are presented in this report and are based on popular participation in environmental management. massive use of the communications media, participation by schoolchildren, constant contact with the population, and integration of various public agencies are the program's priorities. although th ... | 1998 | 9700231 |
| aedes aegypti (diptera: culicidae) movement influenced by availability of oviposition sites. | marked aedes aegypti (l.) (5-6 d old) were released inside 2 groups of 5 houses (100 females per house) in a residential community in florida, pr, to compare behavior of gravid females at sites where oviposition containers were absent to sites where containers were abundant (i.e., 2 tires and 10 ovipots were added to each yard). two sequential releases were made so that both groups of houses were evaluated with oviposition containers removed and added. mosquitoes resting inside the 10 release ho ... | 1998 | 9701948 |
| predation on aedes aegypti (diptera: culicidae) by theridion rufipes (araneae: theridiidae) in puerto rico. | theridion rufipes lucas was found in rearing cages of aedes aegypti (l.) in the school of medicine, san juan, pr. from the 1970s to the 1990s, the spiders had a continuous existence in the cages and in the room outside the cages. a single spider eventually could eliminate all the mosquitoes in a cage. the behavior of the mosquitoes in the web was observed by isolating spiders individually in glass or plastic flasks. when a mosquito comes in contact with the beads of sticky silk on the web, it is ... | 1998 | 9701953 |
| typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase pcr. | in recent years, dengue viruses (serotypes 1 to 4) have spread throughout tropical regions worldwide. in many places, multiple dengue virus serotypes are circulating concurrently, which may increase the risk for the more severe form of the disease, dengue hemorrhagic fever. for the control and prevention of dengue fever, it is important to rapidly detect and type the virus in clinical samples and mosquitoes. assays based on reverse transcriptase (rt) pcr (rt-pcr) amplification of dengue viral rn ... | 1998 | 9705406 |
| multiple host-feeding and biting persistence of aedes aegypti. | the effects of host activity, host defensive behaviour and biting persistence on multiple host-feeding by aedes aegypti (l.) were investigated, in laboratory conditions, on human volunteers. in four different settings, four or five volunteer hosts displayed inactive, mildly active, mildly defensive and highly defensive behaviour. into each of the four trials, each with 20 replicates, a single mosquito was released for a period of 10 min. in all settings, the median number of hosts sought per mos ... | 1998 | 9713547 |
| development of an indicator to evaluate the impact, on a community-based aedes aegypti control intervention, of improved cleaning of water-storage containers by householders. | householders in a community-based programme to control dengue in el progreso, honduras, are being encouraged to improve the cleaning of the water-storage containers in which many of the vectors thrive. the objective of the present study was to develop an indicator of the change occurring in human behaviour. traditional aedes aegypti larval indices do not differentiate between containers in which all the immature stages are present and those which hold only first- and second-instar larvae. howeve ... | 1998 | 9713548 |
| domestic aedes aegypti breeding site surveillance: limitations of remote sensing as a predictive surveillance tool. | this project tested aerial photography as a surveillance tool in identifying residential premises at high risk of aedes aegypti breeding by extending the use of a recently developed, ground-based, rapid assessment technique, the modified premise condition index (pci2). during 1995, we inspected 360 premises in townsville, australia for ae. aegypti breeding, and pci2 scores were recorded. the pci2 values were also estimated from 1:3,000 color and infrared aerial photograph interpretation for the ... | 1998 | 9715943 |
| antibody to a cdna-derived calreticulin protein from amblyomma americanum as a biomarker of tick exposure in humans. | the antibody responses of human and animal hosts were studied to determine the utility of antibody against recombinant tick calreticulin (rtc), a cdna-derived protein isolated from salivary glands of amblyomma americanum l., as a biologic marker of tick exposure. rabbits fed upon by either a. americanum or dermacentor variabilis say developed significant anti-rtc antibody responses, as measured by both elisa and immunoblot assay. in contrast, gerbils exposed to aedes aegypti did not develop anti ... | 1998 | 9715947 |
| bacillus thuringiensis and its pesticidal crystal proteins. | during the past decade the pesticidal bacterium bacillus thuringiensis has been the subject of intensive research. these efforts have yielded considerable data about the complex relationships between the structure, mechanism of action, and genetics of the organism's pesticidal crystal proteins, and a coherent picture of these relationships is beginning to emerge. other studies have focused on the ecological role of the b. thuringiensis crystal proteins, their performance in agricultural and othe ... | 1998 | 9729609 |
| determination of dengue virus serotypes in individual aedes aegypti mosquitoes in colombia. | adult aedes aegypti mosquitoes were collected in puerto triunfo, central colombia, where dengue is endemic, during a six month period. viral infection within the head of each individual mosquito was identified by an immunofluorescent assay (ifa) using a flavivirus-specific monoclonal antibody. the dengue virus serotype, present in each flavivirus-positive specimen, was then determined in portions of the remaining thorax using ifas with serotype-specific monoclonal antibodies. among 2065 female a ... | 1998 | 9737600 |
| comparative repellency of commercial formulations of deet, permethrin and citronellal against the mosquito aedes aegypti, using a collagen membrane technique compared with human arm tests. | a collagen membrane technique, based on the membrane blood-feeding system of cosgrove et al. (1994), was used to compare repellents against aedes aegypti mosquitoes. repellency was defined in terms of inhibition of probing (ed50 and ed90) after 5 min exposure. a direct comparison was made with repellency from probing after 5 min on five male volunteers. four repellent products were compared with technical deet as the standard. the liquid formulations tested were: autan (20% deet; bayer); repel p ... | 1998 | 9737601 |
| thanaka (limonia acidissima) and deet (di-methyl benzamide) mixture as a mosquito repellent for use by karen women. | the prevention and treatment of drug-resistant malaria is becoming increasingly difficult. on the thai-myanmar border multi-drug resistant strains of falciparum malaria are increasing and, because the malaria vector anopheles bite outdoors during early evening, insecticide house-spraying or impregnated bednets provide only limited protection. therefore, the protective efficacy of repellent formulations containing di-methyl benzamide (deet) and permethrin against local vectors was estimated, when ... | 1998 | 9737602 |
| biochemical monitoring of organophosphorus and carbamate insecticide resistance in aedes aegypti mosquitoes from trinidad. | 1998 | 9737606 | |
| distribution of potential dengue vectors in major townships along the national highways and trunk roads of northeast india. | surveys were conducted in some townships along the national highways and trunk roads of northeast india to detect breeding of aedes mosquitos in used/waste tire dumps piled outdoors by the tire repairing shops during summer season of 1996-1997. the breeding of both the potential vectors of dengue, viz. aedes aegypti and ae. albopictus were detected, prevalence rate being in the range of 30.0-88.0 (ci = container index value). the preponderance of ae. aegypti was considerably much higher than tha ... | 1998 | 9740295 |
| estimation of predation by the larvae of toxorhynchites splendens on the aquatic stages of aedes aegypti. | predation by instars of toxorhynchites splendens on aquatic stages of aedes aegypti was studied by estimating functional response parameters such as attack rate (a') and handling time (th) in the laboratory. the predator displayed typical type-ii functional response, similar to that of most insect predators when presented with increasing densities of any given size class of prey. second instar predator attacked prey significantly at higher rate than the other instars. small prey were attacked at ... | 1998 | 9740296 |
| similarities to a line element shared by anopheline and culicine mosquitos map to the distal end of dihydrofolate reductase amplicons in aedes albopictus mosquito cells. | to extend our understanding of amplicon structure in methotrexate-resistant mtx-5011-256 aedes albopictus mosquito cells, we examined a series of cosmids containing genomic dna corresponding to the unique 3'-end of the type 1 dihydrofolate reductase amplicon. cosmid pwed118 contained five ecori fragments ranging from 2 to 5 kb (a, b, c, f, g) that hybridized to cdna from methotrexate-resistant cells. of these, fragments b and f hybridized weakly to first-strand cdna from sensitive cells and shar ... | 1998 | 9753771 |
| the introduction into bacillus sphaericus of the bacillus thuringiensis subsp. medellin cyt1ab1 gene results in higher susceptibility of resistant mosquito larva populations to b. sphaericus. | the fragment containing the gene encoding the cytolytic cyt1ab1 protein from bacillus thuringiensis subsp. medellin and its flanking sequences (i. thiery, a. delécluse, m. c. tamayo, and s. orduz, appl. environ. microbiol. 63:468-473, 1997) was introduced into bacillus sphaericus toxic strains 2362, 2297, and iab872 by electroporation with the shuttle vector pmk3. only small amounts of the protein were produced in recombinant strains 2362 and iab872. the protein was detected in these strains onl ... | 1998 | 9758818 |
| dna binding and transactivation characteristics of the mosquito ecdysone receptor-ultraspiracle complex. | the steroid hormone 20-hydroxyecdysone is a key regulatory factor, controlling blood-meal triggered egg maturation in mosquitoes. to elucidate the ecdysone hierarchy governing this event, we cloned and characterized the ecdysone receptor (aaecr) and the nuclear receptor ultraspiracle (aausp), a retinoid x receptor homologue, from the mosquito, aedes aegypti, which form a functional complex capable of ligand and dna binding. here we analyzed the dna-binding properties of the aaecr.aausp heterodim ... | 1998 | 9765285 |
| sequence of the cry11bb11 gene from bacillus thuringiensis subsp. medellin and toxicity analysis of its encoded protein. | the nucleotide sequence of the cry11bb1 gene from bacillus thuringiensis subsp. medellin was determined. the corresponding protein has a deduced molecular mass of 88.2 kda, and is 60.9% and 83% identical to the proteins cry11aa1 and cry11ba1, respectively. the cry11bb1 protein contains five repetitive blocks of 16 amino acids at the c terminal part. it is highly toxic to first instar laboratory reared aedes aegypti, anopheles albimanus and culex quinquefasciatus larvae. | 1998 | 9774743 |
| field evaluation of mesocyclops longisetus (copepoda: cyclopoidea) for the control of larval aedes aegypti (diptera culicidae) in northeastern mexico. | field trials of the predacious copepod mesocyclops longisetus thiubaud were conducted in northeastern mexico to determine the effectiveness of this species to control larval aedes aegypti (l.) populations and to survive and reproduce in nature. groups of 200, 50, and 50 ovigerous m. longisetus females were inoculated into 200-liter metal drums, discarded tires, and cemetery flower vases, respectively, which are 3 of the more important aedes breeding sites in this area. larvae were sampled at 15- ... | 1998 | 9775596 |
| oviposition attraction and repellency of aedes aegypti (diptera: culicidae) to waters from conspecific larvae subjected to crowding, confinement, starvation, or infection. | as the biomass of aedes aegypti (l.) larvae increased in relation to the volume of rearing waters, oviposition attraction of these waters to conspecific, gravid females first rose to a peak and then declined. further increases in biomass rendered waters strongly repellent. comparable responses were elicited by a decrease in the volume of rearing waters or an increase in the relative size or number of mosquito larvae. low volumes of water reduced oviposition attraction and increased repellency, w ... | 1998 | 9775609 |