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bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads.solubilized crystals of bacillus thuringiensis var. israelensis were 7000 times less toxic to aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. a procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. the latex bead assay should make it possible to analyze the structure and mode of action of the mosquito t ...19846701520
a large transmissible plasmid is required for crystal toxin production in bacillus thuringiensis variety israelensis.bacillus thuringiensis var. israelensis (bti), serotype 14, which produces parasporal crystals toxic to certain dipteran larvae, was analyzed by agarose gel electrophoresis and found to contain a complex plasmid array. eight plasmids were detected, with approximate sizes of 3.3, 4.2, 4.9, 10.6, 68, 75, 105, and 135 mda, as well as a plasmidlike linear dna element of approximately 10 mda. partially cured mutants of bti implicated the 75-mda plasmid in crystal production. fifteen independently iso ...19846709732
the effect of humidity on the transmission of brugia pahangi infective larvae to mammalian hosts by aedes aegypti.the transmission of brugia pahangi by aedes aegypti to the mammalian host was compared at low and high humidity. there was no statistical difference between the number of infected mosquitoes feeding or the egress of infective larvae from these mosquitoes at high or low humidity. the penetration of the host by the infective larvae was however significantly greater (p less than 0.05) at high than at a low humidity.19846710572
plasmodium gallinaceum: erythrocyte factor essential for zygote infection of aedes aegypti.zygotes of plasmodium gallinaceum, fertilized in vitro and fed to aedes aegypti mosquitoes through a membrane, formed oocysts only when a substance in the cytoplasm of uninfected erythrocytes was present. the relation between erythrocyte volume and infectivity was linear (1:1.2) up to a 50% hematocrit. the intraerythrocytic substance was both nondialyzable and poorly soluble in plasma. by carboxymethyl cellulose chromatography, cytoplasmic constituents eluted at ph 8.6 supported the same infecti ...19846714357
[comparison of the flight capability of plasmodia-infected and plasmodia-free aedes aegypti]. 19846717377
[effect of the mosquito cage temperature and the blood of the host food source on the formation and breakdown of the peritrophic membrane in female aedes aegypti l. (diptera, culicidae)]. 20096717378
the use of mosquitoes (aedes aegypti) to detect ciguatoxin in surgeon fishes (ctenochaetus striatus).a new animal assay to detect ciguatoxin in fishes was developed. mosquitoes were subjected to intrathoracic injection of serial dilutions of ciguatoxin crude extracts. toxicity of the fishes was expressed as the mosquito ld50 (g of flesh per mosquito). a significant correlation between the mosquito bioassay and the mouse bioassay performed on the same extracts was shown (r = 0.72, n = 80). we were able to detect 96% of the toxic fishes, while 91% of the fishes non-toxic in mice were also non-tox ...19846719474
a mosquito-virulent bacillus sphaericus in adult simulium damnosum from northern nigeria.from newly emerged adult simulium damnosum from kaduna river, northern nigeria, a mosquito-virulent strain of bacillus sphaericus was isolated. in another four cases non-virulent b. sphaericus were present. the isolate exerted a lc50 of 50 spores/ml for culex pipiens l4-larvae and was less virulent for anopheles stephensi (400 sp/ml) and aedes aegypti (800 sp/ml).19846720123
plasmodium gallinaceum: density dependent limits on infectivity to aedes aegypti.in acute, blood-induced infections of chickens, the malarial parasite plasmodium gallinaceum is most infective to the mosquito aedes aegypti 1 day before gametocyte numbers peak. in an effort to account for this disynchrony , daily changes in parasite infectivity, parasitemia, hematocrit, and hemoglobin were measured during the course of infections. three events were correlated with the loss of infectivity: (1) in the 24 hr between park infectivity and peak gametocytemia , schizont-induced hemol ...19846723894
[ecology of dengue and aedes aegypti. preliminary research. 2]. 19846729600
genetic heterogeneity among caribbean populations of aedes aegypti.using starch gel electrophoresis, we have analyzed genetic variation at 11 loci in 18 caribbean collections of aedes aegypti. our results show that, while there is some relationship between geographic proximity and genetic distance, the overall picture among islands is one of gene frequency patchiness , with some collections clearly not conforming to any geographic pattern. we attribute this to the combined effects of high rates of gene flow among islands and with the mainland american continent ...19846731681
experimental aerial spraying with ultra-low-volume (ulv) malathion to control aedes aegypti in buga, colombia. 19846733345
blood feeding affects age-related changes in the host-seeking behavior of aedes aegypti (diptera: culicidae) during oocyte maturation. 19846748001
nondevelopment of wuchereria bancrofti in nude (congenitally athymic) mice.infective larvae of wuchereria bancrofti (nematoda: filarioidea) were harvested from aedes aegypti mosquitoes fed in vitro on microfilaremic blood of haitian volunteers. larvae were inoculated either into the subcutaneous tissue (sc) or peritoneal cavity (ip) of congenitally athymic (nude) mice, c3h/hen (nu/nu). initially 15 male and two female mice received larval doses from 38-180, and necropsy was done between 11 and 75 days post-inoculation. no worms were found at necropsy. four additional m ...19826751107
comparative study of hemocytes and associated cells of some medically important dipterans.the aim of this work is to study, characterize, and compare different morphological types of hemocytes of glossina austeni, g. morsitans, calliphora erythrocephala, stomoxys calcitrans, lucilia sericata, aedes aegypti, and culex quinquefasciatus. this information is intended to provide a basis for future studies of the cellular defense mechanisms of these dipterans. seven morphological types of hemocytes were identified by phase-contrast optics: prohemocytes, plasmatocytes, thrombocytoids, granu ...19826764649
transovarial transmission of yellow fever virus in stegomyia mosquitoes.transovarial transmission of two strains of yellow fever virus was demonstrated in three colonized geographical strains of aedes aegypti following infection by intrathoracic inoculation. infected progeny were detected in f1 offspring from only the first three ovarian cycles (ovc). the overall minimum filial infection rate for the first three ovc was 1:596; rates were loser in the second and third ovc. virus survived in eggs for longer than 4 months and was recovered from progeny of three immersi ...19806766282
dengue-2 vaccine: viremia and immune responses in rhesus monkeys.studies were undertaken in indian rhesus monkeys (macaca mulatta) to determine the safety, potency, immunogenicity, and mosquito infectivity of a small-plaque, temperature-sensitive variant of dengue type 2 (den-2) virus, a vaccine candidate. fifteen monkeys were inoculated subcutaneously with the vaccine virus, ten receiving 10(3.1) plaque-forming units (pfu) and five receiving 10(4.5) pfu. after primary immunization, viremia was detected in only one monkey, a recipient of the higher dose of va ...19806766903
comparative susceptibility of sterilized and genetically-defined strains of aedes aegypti to dirofilaria repens. 19816798102
cryopreservation of the microfilariae of brugia malayi, dirofilaria corynodes, and wuchereria bancrofti.methods are presented for the cryopreservation of a sheathed microfilaria, brugia malayi, and an unsheathed species, dirofilaria corynodes. the former survived best when frozen at the rate of -0.8 degree or -0.5 degree c per minute using 9% dimethyl sulfoxide (dmso) as the cryopreservative. approximately 52-79% of the thawed microfilariae developed to the third stage in aedes aegypti mosquitoes versus 79% of the unfrozen specimens. for d. corynodes the optimum freezing rate was -2 degrees or -5 ...19836824119
epidemiologic investigations of dengue infection in mexico, 1980.a binational investigation was conducted in two mexican cities in 1980 to study epidemiologic characteristics of dengue. two study areas were selected in each of the cities (merida and tampico); in each area, in february and in september, sanitarians recorded information concerning abundance of aedes aegypti, and public health nurses obtained blood specimens and clinical information from residents. ninety-nine individuals (24% of the study population) showed serologic evidence of recent dengue 1 ...19836829561
plasmodium gallinaceum: avian screen for drugs with radical curative properties.existing primary screens for radical curative antimalarial drugs fail to adequately detect many compounds which affect the latent, exoerythrocytic hypnozoite, the stage of the parasite responsible for relapse. at the same time, these screens falsely identify a wide range of compounds with no radical curative activity. the avian malaria, plasmodium gallinaceum, and aedes aegypti mosquitos were used in a screen which measures the effects of candidate compounds on gametocytes and their development ...19836832279
larvicidal activity of bacillus thuringiensis subsp. israelensis, serovar h14 in aedes aegypti: histopathological studies. 19836841993
[repellent properties of mannich bases and cresol and phenol derivatives for aedes aegypti l. mosquitoes and the flea xenopsylla cheopis roths]. 19836843496
[effect of the larval density of aedes aegypti mosquitoes on their mortality as affected by bacillus thuringiensis berl. preparations]. 19836843501
transovarial transmission of dengue 2 virus by aedes aegypti in nature.dengue 2 virus was recovered from three of 123 pools of naturally infected aedes aegypti larvae (6,200 insects) collected from water containers in rangoon. the virus was also isolated from two of 76 pools (7,730 mosquitoes) of male ae. aegypti, collected as larvae and reared in the laboratory to adults. minimum field infection rates among these two groups of mosquitoes were 1:2,067 and 1:3,865, respectively. insect pools were inoculated into toxorhynchites splendens mosquitoes and dengue viral a ...19836859404
glutathione s-transferase activities in the yellow-fever mosquito [aedes aegypti (louisville)] during growth and aging.our previous findings [hazelton & lang (1978) fed. proc. fed. am. soc. exp. biol. 37(6), 2378 (abstr.)] demonstrated aging-specific changes in glutathione concentrations in the yellow-fever mosquito [aedes aegypti (louisville)]. a possible mechanism could be increased utilization via glutathione s-transferase. thus glutathione s-transferase activities were measured in mosquito samples from the entire life span, including growth, maturity and senescence. methods were validated for the quantitativ ...06860300
glutathione biosynthesis in the aging adult yellow-fever mosquito [aedes aegypti (louisville)].our previous findings [hazelton & lang (1978) fed. proc. fed. am. soc. exp. biol. 37(6), 2378 (abstr.)] demonstrated a senescence-specific decrease in glutathione (gsh) concentration in the yellow-fever mosquito aedes aegypti (louisville)]. as a possible mechanism for this change, gsh biosynthesis was investigated in adult mosquitoes of different ages through the life-span. biosynthesis was measured as the incorporation rate of [14c]glycine or [14c]cystine into glutathione. essential information ...06860301
[action of crystals of bacillus thuringiensis var. israelensis on the midgut of aedes aegypti l. larvae, studied by electron microscopy].ingestion of bacillus thuringiensis var. israelensis crystals by aedes aegypti larvae is followed by midgut epithelium disruption. earliest ultrastructural changes consist of an enlargement of intra- and intercellular spaces in the basal region of the cell. endoplasmic reticula disintegrate by forming spherical structures which increase in size during intoxication. mitochondria are transformed at first into a condensed form, then become swollen with the disappearance of internal cristae. in the ...19836870088
toxicity of bacillus thuringiensis var. israelensis crystals to aedes aegypti larvae: carbonate reversal.the toxicity of purified bacillus thuringiensis var. israelensis crystals to larvae of aedes aegypti could be reversed 100-fold by levels of k(2)co(3) as low as 0.15%.19836870244
an osmoregulatory syncytium and associated cells in a freshwater mosquito.in embryological terms the anal papillae are the product of eversion of the hindgut tissues. the rectum and the anal papillae have the same origin and have a marked structural similarity. the insect hindgut is very labile being able to produce salt transporting or 'chloride cells' from any of the tissues of which it is composed. the hindgut consists of four distinct regions: the ileum and part of the anal canal have a mechanical function, the rectum and the posterior anal canal contain transport ...19836879594
effect of trypsin and neuraminidase on the infectivity of plasmodium gallinaceum sporozoites.an investigation was carried out to examine the effect of trypsin and neuraminidase on the infectivity of plasmodium gallinaceum sporozoites. the sporozoites were obtained from freshly dissected salivary glands of aedes aegypti: their infectivity was assayed by their ability to produce a patent parasitaemia following their inoculation into day-old chicks. infectivity was abolished by treatment of the sporozoites with trypsin at a concentration of 100 micrograms ml-1 for 30 minutes at 30 degrees ...19836882061
the regulation of preimaginal populations of aedes aegypti l. (diptera: culicidae) on the kenya coast. i. preimaginal population dynamics and the role of human behaviour.on the kenya coast aedes aegypti breeds in pots maintained indoors for water storage. pupal numbers vary greatly from one pot to another. the rhythm of water replenishment and the accidental introduction of food into the pots regulate pupal numbers. when water replenishment occurs more often than once per week, pupal numbers are small. when replenishment occurs less often, both low and high pupal numbers may be observed. the presence or absence of larval food distinguishes pots of high versus lo ...19836882066
inheritance of brown-eye and colourless-eye in the mosquito aedes aegypti.an autosomal recessive gene bw (probably closely linked to y on chromosome 2) gives a brown eye colour. when this gene and the sex-linked gene re (red-eye) are both homozygous, a colourless-eye results. brown-eye shows good viability and penetrance, and it has been maintained as a pure stock. colourless-eye shows 87% penetrance in both sexes, and the females are low in viability and fertility. a colourless-eye stock has been maintained by crossing colourless-eyed males to their heterozygous red- ...19836882068
production of bacillus sphaericus strain 1593 primary powder on media made from locally obtainable nigerian agricultural products.five media, formulated from dried cow blood, mineral salts, and seeds from four species of legumes, were assessed for growth, sporulation, and insecticidal properties of bacillus sphaericus strain 1593. bacterial powders, prepared from broth, were assayed against culex quinquefasciatus, anopheles gambiae, and aedes aegypti. good growth and sporulation were obtained with all the media. the highest number of viable cells and spores per mililitre (8.6 x 10(8) and 8.1 x 10(8] were obtained in media ...19836883226
macrogeographic genetic variation in a human commensal: aedes aegypti, the yellow fever mosquito. 19836884770
brugia pahangi: growth improvement with lecithin in the diet of axenically reared hosts, aedes aegypti. 19816894428
anti-gamete monoclonal antibodies synergistically block transmission of malaria by preventing fertilization in the mosquito.experiments from our laboratory previously demonstrated that infected chickens immunized with gametes of the avian malaria parasite plasmodium gallinaceum were no longer infectious to the mosquito vector aedes aegypti and that this transmission-blocking immunity was mediated by antibody. to identify those antigens that are the targets of transmission-blocking immunity, hybrid mouse cell lines secreting monospecific antibodies to surface antigens on male and female gametes of p. gallinaceum have ...19806935685
linkage relationships between an esterase locus and group ii markers in the yellow fever mosquito, aedes aegypti (diptera: culicidae). 19816945437
dengue haemorrhagic fever--a public health problem and a field for research.dengue haemorrhagic fever/dengue shock syndrome (dhf/dss) is an enigmatic and growing public health problem which is confined at present to countries of south-east asia. since 1956, over 350 000 patients have been hospitalized and nearly 12 000 deaths have been reported. dengue viruses, a group of four flaviviruses, are transmitted to man by aedes aegypti. currently, dengue viruses are actively transmitted in 61 countries which circle the globe in the tropical zone and have a combined population ...19806966540
role of a modified ovitrap in the control of aedes aegypti in houston, texas, usa.a modified ovitrap from which larvae could not escape was employed for one year for the control of aedes aegypti at blossom heights, houston, texas. at the end of this period, the breteau index in the ovitrap area had declined by 36%, in contrast to a sharp increase of nearly 500% in the non-ovitrap area. the premises index in the ovitrap area remained unchanged, while the index in the non-ovitrap area increased by 440%. the problems associated with this control campaign and the use of these "au ...06980740
scanning electron microscope study on larvae of wuchereria bancrofti from the vector and from experimental rodent hosts.the surface structures of larvae of wuchereria bancrofti from liberia were examined by scanning electron microscopy. for this study microfilariae from man, first-stage, second-stage, and third-stage larvae from the vector aedes aegypti, and fourth-stage larvae from the experimental rodent hosts mastomys natalensis and meriones unguiculatus were available. from these larvae the structure of the anterior ends, the cuticle in the midbody regions, and the posterior ends are presented. as an obvious ...19807003854
morphometric parameters of the midgut cells of aedes aegypti l. (insecta, diptera) under various conditions.previous morphometric or biochemical investigations have yielded different data on the distribution of free and membrane-bound ribosomes in midgut cells of aedes aegypti. in the present paper ribosomal distribution has been morphometrically analysed to determine whether different mosquito strains, different food and different narcosis used in these previous studies, and/or methodological errors, could account for the different results. most of the cellular parameters in the stomach epithelium of ...19817023689
[action of development inhibitors on aedes aegypti l. mosquitoes and rhodnius prolixus stal. bugs. iv. the activity of precocenes]. 20157024772
morphogenesis of yellow fever virus in aedes aegypti cultured cells. i. isolation of different cellular clones and the study of their susceptibility to infection with the virus.we report the isolation of 19 cellular clones of aedes aegypti and analyze their susceptibility to infection with yellow fever virus, in comparison with the uncloned cells. four types of clones were found, different in their morphology, their metabolism and their virus production. the c 17 clone produces the most virus, not only with respect to the other aedes aegypti clones, but also to other uncloned arthropod cells published in the literature. a cytopathic effect exists in the virus infected ...19817025663
ultrastructural damage of in vitro cultured ookinetes of plasmodium gallinaceum (brumpt) by purified proteinases of susceptible aedes aegypti (l.).previous in vivo studies have implicated trypsin-like proteinases in the destruction of ookinetes of plasmodium gallinaceum in the gut of the susceptible mosquito aedes aegypti (gass 1977). an in vitro study has shown that the ookinetes are destroyed by crude extracts of blood-fed a. aegypti and that this destruction is largely inhibited by an inhibitor of mosquito proteinases, lima bean trypsin inhibitor (gass and yeates 1979). in the present study ookinetes of p. gallinaceum have been incubate ...19817034390
genetic analysis of abnormal male sexual development in aedes aegypti and ae. mascarensis backcross progeny.when male hybrids of aedes aegypti females and a. mascarensis males were backcrossed to a. aegypti females, 32.8 percent of the male progeny exhibited abnormal sexual development, including failure of the terminalia to rotate, a split sternite of the eighth abdominal segment with partially duplicated telomeres, or feminization that gives rise to sterile intersexes. observations made on three morphological marker loci and five isozyme loci with characteristic electromorphs in the two parental spe ...20107069190
host behaviour and mosquito feeding success: an experimental study.the susceptibility of laboratory rabbits to attack by mosquitoes (aedes aegypti) is found to be closely linked to their grooming activity. prevention of grooming significantly increases both the percentage of mosquitoes feeding and the size of blood meals. grooming responses intensify with parasite density, and lead to a slight decline in parasite feeding success and blood meal size. grooming efficiency varied between individual rabbits and also a consequence of experience, with rabbits improvin ...19827080144
utilization and metabolism of dietary sterols in the honey bee and the yellow fever mosquito.the honey bee, apis mellifera, does not convert c28 and c29 phytosterols to cholesterol as found in most previous studies of phytophagous or omnivorous insects, but instead the workers and queens selectively transfer 24-methylenecholesterol, sitosterol and isofucosterol from their endogenous sterol pools to the brood larvae regardless of the sterol in the worker diet. administering radiolabeled sterols by feeding and injection has made it possible to trace this selective transfer through a secon ...19827087696
the infectivity of microfilariae of brugia pahangi of different ages to aedes aegypti.by transferring microfilariae of brugia pahangi which had been born over a 24-hour period in the peritoneal cavities of jirds (meriones unguiculatus) to the blood circulation of other jirds, infections of known age were tested for their ability to develop into third-stage larvae (l3) in mosquitoes aedes aegypti. microfilariae less than three days old were not able to develop to l3. microfilariae which had been in circulation for three days to six months were capable of developing if ingested.19827096971
geographic and temporal patterns of genetic variation of aedes aegypti in new orleans.populations of aedes aegypti were collected in 1976 and 1979 from a number of different areas of new orleans and analyzed for genetic variation of 12 isozyme loci. although six loci were polymorphic, the majority of loci showed no significant genetic differentiation over time or between regions. the greatest amount of genetic differentiation was found between populations bordering the mississippi river and the two inland areas, mid-city and mount olivet. the low amount of genetic heterogeneity a ...19827102920
[on an outbreak caused by dengue type 4 virus, in thio, new caledonia. epidemiological and clinical aspects (author's transl)].after imported cases, a dengue outbreak, caused by type 4 virus, affected 11 per cent of the habitants of thio, new caledonia. distribution of cases by sex, by ethnic and age group, by locality and by week is established on cases confirmed by serology and/or by virus isolation. clinical aspect was classical but liver and digestive tract of patients were frequently affected. five haemorrhagic forms were observed. aedes aegypti was found in each visited house; pooled specimens were found infected ...19827105285
juvenile hormone and the development of ovarian responsiveness to a brain hormone in the mosquito, aedes aegypti. 19827106542
susceptibility of culex quinquefasciatus, aedes aegypti, anopheles culicifacies and anopheles stephensi against insecticides. 19827106890
linkage of an idh locus with sex and two lethals in aedes aegypti.a previously unmapped isocitrate dehydrogenase (dh) locus has been mapped on chromosome 1 in aedes aedes aegypti. no recombination between this and sex was observed in any of the several hundred instances where crossing over could have been detected. within an isofemale line from daker, two ldh-1 alleles are in linkage disequilibrium with two lethal loci. the two lethals complement one another, and show no recombination with sex or ldh-1. recombination between bz and ldh-1 was demonstrated, plac ...20107108187
infection of aedes aegypti with zygotes of plasmodium gallinaceum fertilized in vitro.female gametes of plasmodium gallinaceum fertilized in vitro, cleaned of all other blood constituents, resuspended in blood, and fed to aedes aegypti through a membrane were infective. at the lowest zygote concentration, 10(4)/ml, nearly every ingested parasite produced an oocyst. as the concentration ingested increased, efficiency to infect diminished, until above 10(7) zygotes/ml the number of oocysts produced became constant. this method should be valuable for determining the nutrient require ...19827119992
estimation of the amount of venezuelan equine encephalomyelitis virus transmitted by a single infected aedes aegypti (diptera: culicidae). 19827120306
field experiments on the control of aedes aegypti and culex quinquefasciatus by toxorhynchites rutilus rutilus (diptera: culicidae). 19827120310
dengue-2 vaccine: infection of aedes aegypti mosquitoes by feeding on viremic recipients.colonized aedes aegypti mosquitoes were fed on voluntary recipients of an experimental, live, attenuated, dengue type 2 (pr 159/s-1) vaccine to estimate the frequency of vector infection and the stability of the virus in mosquitoes. two volunteers were viremic at the time of mosquito feeding, but only two of 114 mosquitoes that took a viremic blood meal became infected with the vaccine virus. strains of virus recovered from the bodies of the mosquitoes and the volunteer's blood retained the temp ...19827149107
dengue-2 vaccine: oral infection, transmission, and lack of evidence for reversion in the mosquito, aedes aegypti.the dengue-2 vaccine virus (s-1), and its parent virus (pr-159), were compared for their ability to infect orally, to replicate in, and subsequently to be transmitted by aedes aegypti mosquitoes. the vaccine virus was markedly less efficient in its ability to infect mosquitoes orally. after ingesting infectious bloodmeals containing 3, 7 to 8.2 log10mid50/ml of the respective viruses, 56% (220/396) of the mosquitoes became orally infected with the parent virus contrasted with 16% (66/397) for th ...19827149108
electrophoretic variability in the phosphatase system of the yellow-fever mosquito, aedes aegypti.alkaline and acid phosphatases in five inbred strains of the yellow-fever mosquito, aedes aegypti, were compared by vertical polyacrylamide gel electrophoresis. 1. polymorphic and monomorphic zones of enzyme activity were observed among both alkaline and acid phosphatases. 2. in the alkaline phosphatase system, seven electrophoretic zones of enzyme activity were detected; all but two of the seven zones showed electrophoretic variability. 3. in the acid phosphatase system, four electrophoretic zo ...19827151398
laboratory evaluation of bacillus thuringiensis var. israelensis serotype h-14 against mosquito larvae with particular reference to aedes aegypti. 19827153478
transmission of venezuelan equine encephalomyelitis virus by aedes aegypti (diptera: culicidae) to mice previously exposed to vector antigens. 19827154017
an efficient floating larval trap for sampling aedes aegypti populations (diptera: culicidae). 19827154026
a kinetic and ultrastructural comparison of alphavirus infection of cultured mosquito and vertebrate cells.vero cells and aedes pseudoscutellaris cells showed rapid production of semliki forest virus (sfv) whereas in aedes aegypti and anopheles stephensi cells no rapid production of sfv was observed. ultrastructurally the only virally induced cell inclusion in early infection was the cytopathic vacuole type 1. later in infection, in mosquito cells, electron-dense bodies appear and budding of new virions appears to be very efficient. in vero cells large accumulations of envelope proteins and nucleocap ...19827154145
well breeding behaviour of aedes aegypti. 19827166362
field trials on the effectiveness of bioresmethrin reslin 10/10 (r) on aedes aegypti. 19827167094
the ultrastructure of the larval malpighian tubules of a saline-water mosquito.the larval malpighian tubules of the saline-water mosquito aedes taeniorhynchus were examined using light and electron microscopy. the tubules contain two cell types; primary cells and stellate cells. primary cells are characterized by their size (70 microns x 70 microns x 10 microns) and an abundance of intracellular membrane-bound crystals. two types of microvilli are found on the luminal surface of the primary cells: (1) small microvilli containing core microfilaments and extensions of endopl ...19827170712
multiplication of coxiella burnetii in aedes aegypti. 19827174009
effects of benzylphenol and benzyl-1,3-benzodioxole derivatives on fertility and longevity of the yellow fever mosquito (diptera:culicidae). 19827175006
distribution and density of aedes aegypti (l) and aedes albopictus (skuse) in sarawak. 19827176997
melanization and encapsulation in aedes aegypti and aedes togoi in response to parasitization by a filarioid nematode (breinlia booliati).in aedes aegypti (singapore strain), s refractory host, there was a marked progressive decline of developing larvae of breinlia booliati during the incubation period. however, in aedes togoi, a susceptible host, the filarial larvae developed normally and the number of larvae remained constant throughout the incubation period. encapsulation and melanization of b. booliati larvae in ae. aegypti and ae. togoi were studied. ae. togoi occasionally mounted a defence reaction to the infection. in ae. a ...19827177704
[distribution of aedes aegypti in china and its comprehensive control]. 19827185486
evaluation of n-toluyl piperidine as an effective repellent against aedes aegypti (l). 19807191409
[dipetalonema dessetae in proechimys oris. ii. evaluation of the model for pharmacologic investigations of antifilarial chemotherapy (author's transl)].dipetalonema dessetae in proechimys oris is a new model of rodent feafilariasis with several interesting features with respect to its utilisation in antifilarial pharmacology. 1. in the first part of these studies, it has been shown that the final host of the filaria, a rodent, was easy to breed. a prolific mosquito aedes aegypti was an effective vector, and with the selected mode of infection i.e. 200 infesting larvae subcutaneously injected, 80% of the rodents were infected. 2. from 90 to 150 ...19827200173
[intake of microfilariae by the vector in the case of a low microfilareamia (author's transl)].the study of the intake of microfilariae of dipetalonema dessetae by aedes aegypti leads to two conclusions with respect to the microfilaraemia: --there is no concentration of microfilariae in the uptake of blood by aedes. --the microfilariae are nearly homogeneously distributed in the vertebrate host blood available to the vector for feeding. such a distribution of microfilariae in the cutaneous blood supply of the host gives the maximum chance for a mosquito to become infected when taking a bl ...19827200752
brugia pahangi: development in aedes aegypti reared axenically on a defined synthetic diet. 19817202630
[attractant properties of peptides for oviparous female aedes aegypti and culex pipiens molestus mosquitoes]. 19807207386
isolation and characterization of mosquito cell membrane glycoproteins.plasma membranes have been purified from an established cell line, mos 20a of aedes aegypti, and analysed for glycoprotein and polypeptide constituents by isoelectric focusing and sodium dodecyl sulphate polyacrylamide gel electrophoresis. a major glycoprotein of molecular weight 110 000 carrying binding sites for concanavalin a and soybean agglutinin has been purified to homogeneity. although located on the cell surface, the 110 kdalton glycoprotein is not labelled by lactoperoxidase-catalysed ...19817213698
steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin.a cultured cell line of the mosquito, aedes aegypti, is sensitive to tunicamycin as expected from the ability of crude membrane preparations to catalyse the formation of n-acetylglucosamine-linked dolichyl pyrophosphate. formation of dolichylphosphomannose was also detected and this reaction was totally insensitive to tunicamycin. incorporation of radioactive mannose into total acid-precipitable glycoproteins was inhibited greater than 90% in whole cells by tunicamycin, while the incorporation o ...19817213699
a preliminary survey of aedes aegypti in selangor, peninsular malaysia. 19807219273
disruption of insect photoreceptor membrane by divalent ions: dissimilar sensitivity of light- and dark-adapted mosquito rhabdomeres.the conditions that lead to the formation of myelin figures in rhabdomere microvilli were studied in the larval ocelli of the mosquito aedes aegypti. these artifacts can result from the addition of divalent ions, such as ca2+, to primary-aldehyde fixatives, but they form subsequently during postfixation with oso4. in light-adapted ocelli, myelin figures are concentrated at the proximal ends of the microvilli along the cytoplasmic margin of the rhabdomere. the severity of the artifact is proporti ...19817226214
acetylcholinesterase activity in an aedes aegypti cell line.considerable acetylcholinesterase (ache) activity was detected in an aedes aegypti established cell line. the enzyme is blocked by 10(-6) m eserine sulfate, displays excess substrate inhibition and slowly hydrolyzes butyrylthiocholine. a 2-fold stimulation of ache activity was shown after 2 days exposure to 3 x 10(-7) m beta-ecdysone. ache activity found in the fresh medium is the contribution of the fetal calf serum portion. a direct relationship between levels of serum and the ache activity in ...19817238835
[effect of conditions and duration of aedes aegypti densonucleosis virus storage on its infective properties]. 19817253970
genetic response of aedes aegypti to different environmental temperatures.three groups of aedes aegypti maintained 8 generations in the laboratory at 20 degrees c, 27 +/- 1 degree c (normal control), and at 35 degrees c, respectively were analyzed for allozyme variability. of the 8 larval loci examined est alpha-1 and lap-2 in generation 8 were still variable, while the other allozymes became uniform. the 35 degrees c population had mean level of heterozygosity of about 6% of individual per locus, less than those observed in the 20 degrees c and the control population ...19817256357
factors affecting syncytial development in aedes pseudoscutellaris cells by dengue viruses.several factors that were suspected of affecting the development of syncytia in cultured aedes pseudoscutellaris cells inoculated with dengue viruses were studied. the results indicated that fresh media (less than 1 week old), low cell density at inoculation (2.8 x 10(5) cells/cm2), and low cell passage level (less than 52 passages) favored the development of syncytia. all three types (1, 2, and 3) of dengue viruses tested could be isolated from human sera by aedes pseudoscutellaris cell culture ...19817258498
genetic aspects of the susceptibility of aedes aegypti to dirofilaria and brugia. 19817268859
morphogenesis of yellow fever virus in aedes aegypti cultured cells. ii. an ultrastructural study.the growth and intracytoplasmic development of two yellow fever virus strains (wild and french neurotropic) were studied in aedes aegypti cells (clone c 17). despite a longer period of latency for the vaccine virus, infected cells appeared similar. the cisternae of the rough endoplasmic reticulum (rer) were swollen and formed vesicles which contained the virus. this rer appeared to be the predominant locus of viral synthesis and maturation. cytopathic effect appeared when the cells were filled w ...19817283005
[ph variations in the midgut of aedes aegypti in relation to bacillus thuringiensis var. israelensis (serotype h14) crystal intoxication].after feeding aedes aegypti larvae with b. thuringiensis var. israelensis crystals the very alkaline ph (greater than or equal to 10) of the anterior midgut remains stable during the first twenty minutes, in spite of important cytological damage. thereafter, the ph drops to surround the neutrality and the death occurs. these results are discussed according to the knowledge on the action of b. thuringiensis var. israelensis.19817296735
a model for the mechanism of action of the repellent deet on aedes aegypti (diptera: culicidae). 19817299789
laboratory transmission of brugia pahangi by nulliparous aedes aegypti (diptera: culicidae). 19817299793
aedes aegypti: a sword of damocles over tropical america. 19817306736
evaluation of methoprene (a juvenile hormone) against culex quinquefasciatus, anopheles stephensi and aedes aegypti. 19817309181
relative activity of bacillus thuringiensis var. kurstaki and b. thuringiensis var. israelensis against larvae of aedes aegypti, culex quinquefasciatus, trichoplusia ni, heliothis zea, and heliothis virescens. 19817320315
infection of aedes aegypti with brugia pahangi administered by enema: results of quantitative infection and loss of infective larvae during blood feeding.a technique for infecting mosquitoes with known numbers of brugia pahangi microfilariae by enema is described. virtually all mosquitoes receiving three microfilariae or more by this route contained infective larvae 10 days later. within a range of 1 to 40 microfilariae, numbers of infective larvae recovered (y) were related to the numbers of microfilariae administered (x) by the equation log10y=0.04 + 0.84 log10x. mosquitoes feeding on sugar for up to 20 days did not lose a significant number of ...19817324102
transmission of ross river virus by aedes polynesiensis and aedes aegypti.laboratory studies were carried out with two geographic strains of aedes polynesiensis and one strain of aedes aegypti to determine whether they could transmit ross river virus (rrv). both species were shown to be good vectors of rrv, but ae. polynesiensis was the most susceptible. ae. polynesiensis represents a new vector for this virus and the epidemiologic implications of rrv spread by both mosquito species are discussed.19817325287
observations on container-breeding mosquitoes in new orleans, louisiana, with an estimate of the population density of aedes aegypti (l.).in a survey of containers infested with aedes aegypti (l.) conducted during september and october 1979 in a substandard residential area of new orleans, la, infestation indices were as follows: house--35, breteau (foci)--85, receptacle--69, block--100, and larval density--169. a mean of 48.5 water-holding containers were found per block. more than 50% of these receptacles were narrow-mouthed containers, e.g., drink bottles, that held an average of less than 0.03 mosquito immatures each. after ex ...19817325289
studies on the breeding habitats and seasonal prevalence of larval population of aedes aegypti (l.) and aedes albopictus (skuse) in dacca city.an one-year study was conducted on the outdoor breeding habitats and seasonal prevalence of larval populations of aedes aegypti and aedes albopictus in ten different locations of dacca city. nine locations were found infested with larvae of either one or both species during the rainy season (may to october) of the year. a total of 1898 containers/sites were surveyed out of which 86 (4.53%) were infested with either one or both species. out of then different kinds of artificial containers and nat ...19807325925
sequential development changes in microfilariae of subperiodic brugia malayi to infective larvae in susceptible strain of aedes aegypti (macdonald). 19817343589
scavenger snail secretion as an ovipositional attractant for aedes aegypti. 19817343593
comparative sensitivity of mosquito inoculation and mammalian cell culture for isolation of some arboviruses in indonesia.the sensitivity of parenteral inoculation of colony reared male aedes aegypti and mammalian cell cultures for isolation of japanese encephalitis virus and dengue virus were compared. the mosquito inoculation technique proved to be more sensitive for the isolation of dengue virus from the sera of febrile patients than did the mammalian cell cultures (vero and bhk21) employed in these studies. mosquito inoculation proved to be no more sensitive that the mammalian system for the isolation of japane ...19817344103
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