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green synthesis of silver-nanoparticles from annona reticulata leaves aqueous extract and its mosquito larvicidal and anti-microbial activity on human pathogens.silver nanoparticles play a important role in controlling mosquito population as well as multi drug resistant pathogens without causing much harm to humans. in the present study was focused on green synthesis of silver nanoparticles against dengue causing vector (aedes aegypti) and pathogens affecting humans. the synthesized silver nanoparticle was confirmed using uv- absorption spectrum range obtained at 416 nm, xrd, ftir and hr-tem analysis were used to determine the silver nanoparticle morpho ...201930581768
the tudor protein veneno assembles the ping-pong amplification complex that produces viral pirnas in aedes mosquitoes.piwi-interacting rnas (pirnas) comprise a class of small rnas best known for suppressing transposable elements in germline tissues. the vector mosquito aedes aegypti encodes seven piwi genes, four of which are somatically expressed. this somatic pirna pathway generates pirnas from viral rna during infection with cytoplasmic rna viruses through ping-pong amplification by the piwi proteins ago3 and piwi5. yet, additional insights into the molecular mechanisms mediating non-canonical pirna producti ...201930566680
container size alters the outcome of interspecific competition between aedes aegypti (diptera: culicidae) and aedes albopictus.aedes aegypti l. and aedes albopictus skuse co-occur in a variety of water-filled containers where they compete for resources. larvae of ae. albopictus skuse often outcompete those of ae. aegypti l., but variation in biotic and abiotic parameters can modify the outcome of this interspecific competition. we tested whether container size can alter the magnitude and direction of intra- and interspecific competition by rearing three ae. aegypti and ae. albopictus larval combinations (100:0, 50:50 an ...201930566608
bioactivity of wild carrot (daucus carota, apiaceae) essential oil against mosquito larvae.invasive alien plants wreak havoc on native ecosystems and using them as a source of biopesticides could improve their management. we examined the toxicity of essential oil of wild carrot (also known as 'queen anne's lace', daucus carota linnaeus), an aggressive invader throughout the united states, against aedes aegypti l., culex pipiens l., and culex restuans theobald larvae. comparisons were made between essential oil extracted from umbels of local populations of wild carrot versus a commerci ...201930566578
mechanisms of pyrethroid resistance in aedes (stegomyia) aegypti from colombia.in colombia aedes (stegomyia) aegypti is the main vector of urban arboviruses such as dengue, chikungunya and zika. this urban mosquito has a well-established capacity to develop insecticide resistance to different types of insecticides (pyrethroids, organochlorides, organophosphates), using multiple resistance mechanisms. an understanding of ongoing resistance mechanisms is critical to determining the activities of vector control programs. in order to identify the biochemical and molecular mech ...201930552882
proteins, transcripts, and genetic architecture of seminal fluid and sperm in the mosquito aedes aegypti.the yellow fever mosquito, aedes aegypti,, transmits several viruses causative of serious diseases, including dengue, zika, and chikungunya. some proposed efforts to control this vector involve manipulating reproduction to suppress wild populations or to replace them with disease-resistant mosquitoes. the design of such strategies requires an intimate knowledge of reproductive processes, yet our basic understanding of reproductive genetics in this vector remains largely incomplete. to accelerate ...201930552291
distinctive regulatory properties of pyruvate kinase 1 from aedes aegypti mosquitoes.female aedes aegypti mosquitoes are vectors of arboviruses that cause diseases of public health significance. the discovery of new metabolic targets is crucial for improving mosquito control strategies. we recently demonstrated that glucose oxidation supports ammonia detoxification in a. aegypti. pyruvate kinase (pk, ec 2.7.1.40) catalyzes the last step of the glycolytic pathway. in most organisms, one or more allosteric effectors control pk activity. however, the kinetic properties and structur ...201930578824
production of tetravalent dengue virus envelope protein domain iii based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.dengue fever is a mosquito (aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. there are four serotypes of the dengue virus (denv 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. to date, the first vaccine, dengvaxia (cyd-tdv), is available after many decades' efforts, but only has moderate efficacy. more effective and affordable vaccines are hence required. plants offer promising vaccine producti ...201930578710
seroprevalence of zika virus among asymptomatic pregnant mothers and their newborns in the najran region of southwest saudi arabia.zika virus (zikv) is a teratogenic flavivirus that can cause microcephaly. its main vector, aedes aegypti, has been previ.ously identified in saudi arabia, but no zikv infection has yet been reported. nevertheless, the country is at risk from zikv because it receives many travelers throughout the year, including pilgrims from zikv-endemic countries.201930531174
co-occurrence of v1016i and f1534c mutations in the voltage-gated sodium channel and resistance to pyrethroids in aedes aegypti (l.) from the colombian caribbean region.knockdown resistance is conferred primarily by non-synonymous mutations that reduce pyrethroids binding to voltage-gated sodium channels. in 2014, kdr mutation v1016i in aedes aegypti populations resistant to pyrethroids was reported for the first time in colombiα, in 2016 another kdr mutation, f1534c, and in 2018 the mutation v419l. nine populations of a. aegypti, previously characterized as being resistant to λ-cyhalothrin, deltamethrin, cyfluthrin and permethrin, were used for this study. gen ...201930520256
differential suppression of persistent insect specific viruses in trans-infected wmel and wmelpop-cla aedes-derived mosquito lines.wolbachia suppresses the replication of +ssrna viruses such as dengue and zika viruses in aedes aegypti mosquitoes. however, the range of viruses affected by this endosymbiont is yet to be explored. recently, novel insect-specific viruses (isvs) have been described from numerous mosquito species and mosquito-derived cell lines. cell-fusing agent virus (flaviviridae) and phasi charoen-like virus (bunyaviridae) persistently infect the ae. aegypti cell line aag2 which has been used for experimental ...201930503908
[new altitudinal registration of aedes aegypti in the region of piura, peru]. 201930517515
coinfection of chikungunya and dengue viruses: a serological study from north western region of punjab, india.dengue and chikungunya (chik) infections appear to be increasing in all parts of india. aedes aegypti mosquitoes are common vectors for dengue virus (denv) and chik virus (chikv). in areas where both viruses cocirculate, they can be transmitted together. there are very few studies discussing the dengue-chik coinfection from punjab region of india. the present study was undertaken to study the clinical features of dengue-chik coinfection and compare with monoinfection.201930498319
volatile composition and biological activities of the leaf essential oil from zanthoxylum limoncello grown in oaxaca, méxico.zanthoxylum limoncello is a native plant from southern mexico which is used as a timber source, condiment and as a traditional medicine. herein, we report on the volatile content of the leaf essential oil and its biological activities. the annual essential oils (2015-2018) contained volatile organic compounds which exhibited a moderate growth inhibitory activity against h. pylori atcc 53504 (mic 121.4-139.7 μg ml-1 ), 26695 (mic 85.5-94.9 μg ml-1 ) and j99 (mic 94.7-110.4 μg ml-1 ). these hydrod ...201930512233
saccharomyces cerevisiae (baker's yeast) as an interfering rna expression and delivery system.the broad application of rna interference for disease prevention is dependent upon the production of dsrna in an economically feasible, scalable, and sustainable fashion, as well as the identification of safe and effective methods for rna delivery. current research has sparked interest in the use of saccharomyces cerevisiae for these applications. this review examines the potential for commercial development of yeast interfering rna expression and delivery systems. s. cerevisiae is a genetic mod ...201930474529
ecotoxicity response of chlorpyrifos in aedes aegypti larvae and lactuca sativa seeds after uv/h2o2 and uvc oxidation.chlorpyrifos (cp) is an organophosphate pesticide widely used in agriculture known to cause neurological and immunological effects in addition to interfering in the reproduction and development of organisms. in this study, cp degradation by uv/h2o2 process and uvc radiation was investigated, and the ecotoxicity and phytotoxicity was evaluated using bioassays of aedes aegypti larvae and lactuca sativa seeds. cp degradation was monitored by hplc-dad, and kinetic parameters were calculated for all ...201930471582
going viral 2019: zika, chikungunya, and dengue.chikungunya and zika virus infections are emerging diseases in the americas, and dengue continues to be the most prevalent arthropod-borne virus in the world. these arbovirus diseases may spread by endemic transmission or as travel-related infections and have rapidly expanded their geographic distribution secondary to vector spread. all 3 share a similar clinical picture that includes a maculopapular rash. zika is characterized by pruritic rash, low-grade fever, and arthralgia. congenital nervou ...201930466692
aedes aegypti vector competence studies: a review.aedes aegypti is the primary transmitter of the four viruses that have had the greatest impact on human health, the viruses causing yellow fever, dengue fever, chikungunya, and zika fever. because this mosquito is easy to rear in the laboratory and these viruses grow in laboratory tissue culture cells, many studies have been performed testing the relative competence of different populations of the mosquito to transmit many different strains of viruses. we review here this large literature includ ...201930465912
autocidal gravid ovitrap incorporation with attractants for control of gravid and host-seeking aedes aegypti (diptera: culicidae).aedes aegypti linnaeus (diptera: culicidae) is a primary vector of multiple arboviruses including zika, dengue, chikungunya, and yellow fever. after incorporating bg lure or bg lure + octenol, the cdc's autocidal gravid ovitrap (ago) was more effective at collecting and controlling host-seeking female ae. aegypti than gravid female mosquitoes. the addition of octenol to the ago did not increase the number of female mosquitoes captured by the ago, compared with the ago alone. the ago baited with ...201930462293
monitoring insecticide resistance profiles of aedes aegypti (diptera: culicidae) in the sunda islands of indonesia based on diagnostic doses of larvicides.this study was conducted to monitor the susceptibility status of aedes aegypti (linnaeus) larvae in the sunda islands of indonesia against various organophosphates and organochlorines. larval bioassay was performed in accordance with the world health organization standard protocol. field-collected and reference strains of ae. aegypti larvae were tested against diagnostic doses of eight larvicides belonging to organophosphates and organochlorines, namely bromophos (0.050 mg/liter), chlopyrifos (0 ...201930462258
past, present and future of aedes aegypti in its south american southern distribution fringe: what do temperature and population tell us?aedes aegypti (diptera: culicidae) (linnaeus) is currently the major threat among arbovirus vectors in the americas. we examined its past, present, and future distribution patterns in the south american fringe in association with environmental and demographic variables at two spatial scales. we updated the database of the occurrence of ae. aegypti per locality and modelled by glmm the past occurrence (until 2000) and its expansion (2001-2017) as a function of air temperature, precipitation, alti ...201930458122
water channel activity of putative aquaporin-6 present in aedes aegypti.aquaporins (aqps) are integral membrane channels that facilitate the bidirectional transport of water and sometimes other small solutes across biological membranes. aqps are important in mediating environmental adaptations in mosquitoes and are considered as a novel target for the development of effective insecticides against mosquitoes. here, we expressed aedes aegypti aqp6 ( aaaqp6) in human embryonic kidney (hek) 293 cells and analyzed the water permeability by a conventional swelling assay, ...201930456765
isolation and growth inhibition potential of entomopathogenic nematodes against three public health important mosquito vectors.the prevalence of mosquito vector borne diseases and the resistance of mosquitoes to conventional pesticides have been of important public concern to the mosquito endemic countries. present study was conducted to identify the native bio-larvicidal potential of the entomopathogenic nematodes; steinernema siamkayai (kpr-4) heterohabditis indica (kpr-8), steinernema glaseri and steinernema abbasi. the isolated nematodes were subsequently cultured and evaluated their larvicidal potential against the ...201930414843
preparation and use of a yeast shrna delivery system for gene silencing in mosquito larvae.the mosquito genome projects facilitated research in new facets of mosquito biology, including functional genetic studies in the dengue and zika virus vector aedes aegypti and the primary african malaria vector anopheles gambiae. rna interference (rnai) has facilitated gene silencing experiments in both of these disease vector mosquito species and could one day be applied as a new method of vector control. here, we describe a procedure for the genetic engineering of saccharomyces cerevisiae (bak ...201930414120
a statewide survey of container aedes mosquitoes (diptera: culicidae) in north carolina, 2016: a multiagency surveillance response to zika using ovitraps.native and invasive container-inhabiting aedes mosquitoes (diptera: culicidae) play important roles in the transmission of endemic and traveler-introduced arboviruses in the united states. in response to the emergence of zika virus into the americas, we surveyed the distribution of container aedes spp. of public health importance within north carolina during 2016 using ovitraps. a seasonal survey was conducted in 18 counties from the mountains to the coast to identify species incriminated in the ...201930380070
inhibition of sodium-hydrogen antiport by antibodies to nha1 in brush border membrane vesicles from whole aedes aegypti larvae.the present research report describes na+/h+ antiport by brush border membrane vesicles isolated from whole larvae of aedes aegypti (aebbmvw). our hypothesis is that acid quenching of acridine orange by aebbmvw is predominantly mediated by na+/h+ antiport via the nha1 component of the aebbmvw in the absence of amino acids and atp. aenha1 is a na+/h+ antiporter that has been postulated to exchange na+ and h+ across the apical plasma membrane in posterior midgut of a. aegypti larvae. its principal ...201930392010
the combinatory effect of cyt1aa flexibility and specificity against dipteran larvae improves the toxicity of bacillus thuringensis kurstaki toxins.cyt1a98 is a novel cytolytic protein, from bupm98 bacillus thuringiensis strain, characterized by its synergistic activity with b. thuringiensis kurstaki toxins against lepidopteran larvae. in this study, we evidenced that cyt1a98 improves the toxicity of b. thuringiensis kurstaki toxins against aedes aegypti larvae. in fact, the strain bns3phtcyt1a98 exhibited a larvicidal activity of about 849-fold of that of bns3phtblue against a. aegypti. the molecular and biochemical characterizations, of c ...201930391590
host blood-meal source has a strong impact on gut microbiota of aedes aegypti.gut microbial communities of mosquitoes can influence vector susceptibility to pathogens, yet the factors that govern their composition remain poorly understood. we investigated the impact of host blood-meal source on gut microbiota of aedes aegypti l. adult mosquitoes were fed on human, rabbit or chicken blood and their gut microbiota compared to those of sugar-fed and newly emerged adults. microbial diversity was significantly reduced in blood-fed and sugar-fed mosquitoes but was restored to t ...201930357406
walter reed at camp lazear: a paradigm for contemporary clinical research.throughout the time of the early settlement and development of north america, there were frequent epidemics of yellow fever. it is thought that ships transporting captured africans likely conveyed both major vectors, the aedes aegypti mosquito and the rna yellow fever virus from africa to north america. infected ships landing in port cities resulted in epidemics that proved impossible to control with conventional interventions. walter reed and the u.s. army commission solved the mystery of the m ...201930327122
bioassay and molecular study for detection of insecticide resistance dengue causing mosquito vectors.nowadays, dengue infection creates a major problem across the country. the vector species carrying dengue infection has progressively started to developed resistance against most of the currently used insecticides. hence, a study was carried out in dengue-endemic areas of assam and arunachal pradesh to find the current situation of insecticide susceptibility status of dengue vectors. based on the previous history of dengue incidence, aedes mosquitoes were collected from dibrugarh, kamrup, sivasa ...201930429402
vector competence analysis of two aedes aegypti lineages from bello, colombia, reveals that they are affected similarly by dengue-2 virus infection.dengue is the second most prevalent vector-borne disease after malaria in colombia. it is caused by dengue virus, an arbovirus that exhibits high epidemic power, which is evidenced by its occurrence in more than 80% of the country, largely because of the extensive dispersion of the mosquito vector aedes aegypti. the existence of two lineages of ae. aegypti has been proposed based on genetic differences at the mitochondrial level, and they have been reported to circulate in similar proportions in ...201930298277
seasonal and spatial distribution of aedes aegypti and aedes albopictus in a municipal urban park in são paulo, sp, brazil.dengue, yellow fever, chikungunya and zika are important arboviruses present in various countries of the world, the etiological agents of which are transmitted to human-beings by the bite of infected females of aedes aegypti and aedes albopictus. biological aspects of these vectors, such as their distribution and abundance, are influenced by climatic variables such as rainfall and temperature. we assess the spatial and seasonal distribution of ae. aegypti and ae. albopictus, during spring 2014 a ...201930282003
hypometabolic strategy and glucose metabolism maintenance of aedes aegypti egg desiccation.the mosquito aedes aegypti is vector of several viruses including yellow fever virus, dengue virus chikungunya virus and zika virus. one of the major problems involving these diseases transmission is that a. aegypti embryos are resistant to desiccation at the end of embryogenesis, surviving and remaining viable for several months inside the egg. therefore, a fine metabolism control is essential to support these organisms throughout this period of resistance. the carbohydrate metabolism has been ...201930266630
vaccination and immunization strategies to design aedes aegypti salivary protein based subunit vaccine tackling flavivirus infection.flavivirus causes arthropod-borne severe diseases that sometimes lead to the death. the flavivirus species including dengue virus, zika virus and yellow fever virus are transmitted by the bite of aedes mosquitoes. all these viral species target the people living in their respective endemic zone causing a high mortality rate. recent studies show that immune factors present in the ae. aegypti saliva is the hidden culprit promoting blood meal collection, suppressing host immune molecules and promot ...201930219509
the intrinsically disordered c-terminal f domain of the ecdysteroid receptor from aedes aegypti exhibits metal ion-binding ability.the dominant vector of dengue and zika diseases is a female aedes aegypti mosquito. its reproduction is controlled by the formation of an active heterodimer complex of the 20-hydroxyecdysone receptor (ecr) and ultraspiracle protein (usp). although ecr exhibits a structural and functional organization typical of nuclear receptors (nrs), the ecr c-terminus has an additional f domain (aafecr) that is rarely present in the nrs superfamily. the presence of f domains is evolutionarily not well conserv ...201930243841
enhanced mortality in deltamethrin-resistant aedes aegypti in thailand using a piperonyl butoxide synergist.aedes aegypti is the primary vector of dengue viruses in thailand. control of this mosquito continues to rely heavily on use of insecticides in various forms and applications. the synergistic effect of piperonyl butoxide (pbo), combined with deltamethrin against eight populations of ae. aegypti collected from different regions in thailand is presented. the standard who adult contact bioassays found all populations with low to moderate levels of resistance to deltamethrin alone (using a 0.05% dis ...201930287252
a comparison of mosquito densities, weather and infection rates of aedes aegypti during the first epidemics of chikungunya (2014) and zika (2016) in areas with and without vector control in puerto rico.in puerto rico, the first records of the transmission of chikungunya (chikv) and zika (zikv) viruses were confirmed in may 2014 and december 2015, respectively. transmission of chikv peaked in september 2014, whereas that of zikv peaked in august 2016. the emergence of these mosquito-transmitted arboviruses in the context of a lack of human population immunity allowed observations of whether the outbreaks were associated with aedes aegypti (diptera: culicidae) densities and weather. mosquito den ...201930225842
characterization of tolypocladium cylindrosporum (hypocreales, ophiocordycipitaceae) isolates from brazil and their efficacy against aedes aegypti (diptera, culicidae).to survey and to characterize entomopathogenic fungi as natural enemies of mosquitoes in central brazil.201930160316
susceptibility and vectorial capacity of american aedes albopictus and aedes aegypti (diptera: culicidae) to american zika virus strains.the rapid expansion of zika virus (zikv), following the recent outbreaks of chikungunya virus, overwhelmed the public health infrastructure in many countries and alarmed many in the scientific community. aedes aegypti (l.) (diptera: culicidae) and aedes albopictus (skuse) (diptera: culicidae) have previously been incriminated as the vectors of these pathogens in addition to dengue virus. in our study, we challenged low generation ae. aegypti (chiapas, mexico) and ae. albopictus (north carolina, ...201930102327
[first evidence of aedes aegypti in the arequipa region, perú 2016]. 201930183928
vertebrate hosts of aedes aegypti, aedes albopictus, and culex quinquefasciatus (diptera: culicidae) as potential vectors of zika virus in florida.zika virus (zikv), once considered an obscure pathogen, spread rapidly from 2014 to 2016 to become an internationally notifiable condition of major public health concern. the relative importance of various culex and aedes species mosquitoes (diptera: culicidae) in zikv transmission is a topic of debate. quantifying host use is important in determining the vectorial capacity of a mosquito species for transmitting zikv in nature. in the united states, few data are available on host use of aedes ae ...201930165498
evaluation of aib and peg-polymer insect kinin analogs on mosquito and tick gpcrs identifies potent new pest management tools with potentially enhanced biostability and bioavailability.insect kinins modulate aspects of diuresis, digestion, development, and sugar taste perception in tarsi and labellar sensilla in mosquitoes. they are, however, subject to rapid biological degradation by endogenous invertebrate peptidases. a series of α-aminoisobutyric (aib) acid-containing insect kinin analogs incorporating sequences native to the aedes aegypti mosquito aedeskinins were evaluated on two recombinant kinin invertebrate receptors stably expressed in cell lines, discovering a number ...201930107140
pan-american league of associations for rheumatology-central american, caribbean and andean rheumatology association consensus-conference endorsements and recommendations on the diagnosis and treatment of chikungunya-related inflammatory arthropathies in latin america.although mortality rates related with chikungunya (chik) outbreaks in latin america's (la's) dengue-endemic rural and new urban regions are low, dealing with symptoms and sequelae can both produce a significant burden of disease and diminish quality of life-from many months to years-after the acute phase of the infection, with a significant impact on public and individual health.the aim of this work was to establish pan-american league of associations for rheumatology-central american, caribbean ...201930028809
human blood as the only source of aedes aegypti in churches from merida, yucatan, mexico. 201929916450
encapsulation of piper aduncum and piper hispidinervum essential oils in gelatin nanoparticles: a possible sustainable control tool of aedes aegypti, tetranychus urticae and cerataphis lataniae.the encapsulated essential oils (eos) of piper aduncum l. and piper hispidinervum c. dc. in gelatin nanoparticles were evaluated against aedes aegypti linn., tetranychus urticae koch and cerataphis lataniae boisd.201929971785
lippia gracilis essential oil in β-cyclodextrin inclusion complexes: an environmentally safe formulation to control aedes aegypti larvae.one of the most efficient ways to prevent arboviruses, such as dengue fever, yellow fever, chikungunya and zika, is by controlling their vector, the aedes aegypti. because this vector is becoming resistant to most larvicides used, the development of new larvicides should be considered. β-cyclodextrin (β-cd) complexes have been investigated as an interesting way of enabling the use of essential oils in water as larvicides. this study comprised the development of lippia gracilis essential oil (lge ...201929998608
efficacy of lysinibacillus sphaericus against mixed-cultures of field-collected and laboratory larvae of aedes aegypti and culex quinquefasciatus.lysinibacillus sphaericus (bacillales: planococcaceae) is a spore-forming bacillus used for the biological control of mosquitoes (diptera: culicidae) due to its larvicidal activity determined by various toxins and s-layer protein produced either during sporulation or by the vegetative cell. aedes aegypti and culex quinquefasciatus are the vectors of arboviruses that cause tropical diseases representing a current public health problem. both species may coexist in the same larval development sites ...201929784071
overview of dengue and zika virus similarity, what can we learn from the saudi experience with dengue fever?there is high public health alert in the kingdom of saudi arabia concerning zika virus infection. so far, there is no reported outbreak. so are we at risk of this disease? reviewing the literature of recent outbreaks of other infectious diseases in saudi arabia may clarify the situation. it is evident that there is some similarity between zika and dengue regarding vector (aedes aegypti) which is available in the kingdom of saudi arabia. furthermore, they have similar transmission process and the ...201929623022
analysis of cluster-randomized test-negative designs: cluster-level methods.intervention trials of vector control methods often require community level randomization with appropriate inferential methods. for many interventions, the possibility of confounding due to the effects of health-care seeking behavior on disease ascertainment remains a concern. the test-negative design, a variant of the case-control method, was introduced to mitigate this issue in the assessment of the efficacy of influenza vaccination (measured at an individual level) on influenza infection. her ...201929447357
zika virus disease.the zika virus is an arbovirus belonging to the virus family flaviviridae. the virus was isolated in 1947 from a rhesus monkey in the zika forest of uganda. the virus causes sporadic mild human infections in africa and later in asia. however, by 2007 a major shift in its infection pattern was noticed and thousands of human infections were reported in the state of yap and federated states of micronesia. in the last 3 years, major outbreaks have continued to occur and the virus has spread to sever ...201930787745
[coinfection of dengue and chikungunya viruses. narrative review].dengue and chikungunya viruses are transmitted by the female aedes aegypti and aedes albopictus, which are widely distributed in tropical and subtropical areas, facilitating coinfection.201931095186
geometric morphometric analysis of the effect of temperature on wing size and shape in aedes albopictus.wing geometry helps to identify mosquito species, even cryptic ones. on the other hand, temperature has a well-known effect on insect metric properties. can such effects blur the taxonomic signal embedded in the wing? two strains of aedes albopictus (laboratory and field strain) were examined under three different rearing temperatures (26, 30 and 33 °c) using landmark- and outline-based morphometric approaches. the wings of each experimental line were compared with aedes aegypti. both approaches ...201931125148
new records of aedes aegypti and aedes albopictus in eight texas counties, u.s.a. 201931124239
spatial repellency and other effects of transfluthrin and linalool on aedes aegypti and aedes albopictus.in the present study, the effects of two spatial repellents (sr) were determined for aedes aegypti and ae albopictus, the main vectors of dengue, chikungunya, and zika fever. the modular high-throughput screening system (hitss) was used to evaluate the response of both species to transfluthrin and linalool sr at different concentrations. the highest spatial repellency results for ae. aegypti were obtained by transfluthrin to 0.001% with 37.50 ± 4.33%, and for linalool to 10% with 77.50 ± 3.90%. ...201931124222
diet drugs trick mosquitoes into feeling full.after a bloodmeal, aedesaegypti mosquitoes lose interest in bloodfeeding. duvall et al. (cell 2019;176:687-701) determined that the neuropeptide y (npy)-like receptor 7 (npylr7) controls mosquito satiety and also identified six npylr7 drug targets that suppress biting. this work highlights an innovative approach in vector control linking insect behavior to drug discovery.201931122765
excito-repellent activity of β-caryophyllene oxide against aedes aegypti and anopheles minimus.contact irritant and non-contact repellent activities of β-caryophyllene oxide were evaluated against laboratory strains of female aedes aegypti (usda strain), a major arbovirus vector and anopheles minimus (ku strain), a major malaria parasite vector, compared with the synthetic repellent deet, using an excito-repellency test system. β-caryophyllene oxide and deet were tested at concentrations of 0.1, 0.25, 0.5 and 1.0% (v/v). anopheles minimus was found to be more sensitive to β-caryophyllene ...201931121148
identification of molecular determinants of resistance to pyrethroid insecticides in aedes aegypti (diptera: culicidae) populations in california, usa.the first breeding populations of aedes aegypti (linnaeus) were identified in california in 2013, and have since been detected in 13 counties. recent studies suggest two introductions likely occurred, with genetically distinct populations in the central and southern regions of the state. given the threat of dengue, chikungunya, and zika virus transmission, it is imperative to understand if these populations harbor genes that could confer resistance to pyrethrin-based insecticides, known as pyret ...201931121042
uncertainty analysis of species distribution models.the maximum entropy model, a commonly used species distribution model (sdm) normally combines observations of the species occurrence with environmental information to predict the geographic distributions of animal or plant species. however, it only produces point estimates for the probability of species existence. to understand the uncertainty of the point estimates, we analytically derived the variance of the outputs of the maximum entropy model from the variance of the input. we applied the an ...201931120909
insecticide resistance levels and mechanisms in aedes aegypti populations in and around ouagadougou, burkina faso.recent outbreaks of dengue and other aedes aegypti-borne arboviruses highlight the importance of a rapid response for effective vector control. data on insecticide resistance and underlying mechanisms are essential for outbreak preparedness, but are sparse in much of africa. we investigated the levels and heterogeneity of insecticide resistance and mechanisms of ae. aegypti from contrasting settings within and around ouagadougou, burkina faso.201931120874
topological qsar modelling of carboxamides repellent activity to aedes aegypti.aedes aegypti vector control is of paramount importance owing to the damages induced by the various severe diseases that these insects may transmit, and the increasing risk of important outbreaks of these pathologies. search for new chemicals efficient against aedes aegypti, and devoid of side-effects, which have been associated to the currently most used active substance i. e. n,n-diethyl-m-toluamide (deet), is therefore an important issue. in this context, we developed various quantitative str ...201931120598
entomological efficacy of aerial ultra-low volume insecticide applications against aedes aegypti (diptera: culicidae) in mexico.a cluster-randomized controlled trial quantified the entomological efficacy of aerial ultra-low volume (aulv) applications of the insecticide chlorpyrifos against aedes aegypti in puerto vallarta, méxico, during november-october 2017. the trial involved 16 large (1 × 1 km) clusters distributed between treatment-control arms. primary endpoint was the abundance of ae. aegypti indoors (total adults, females, and blood-fed females) collected using prokopack aspirators. after four consecutive weekly ...201931120502
wolbachia strain walba blocks zika virus transmission in aedes aegypti.transinfections of the maternally transmitted endosymbiont wolbachia pipientis can reduce rna virus replication and prevent transmission by aedes aegypti, and also have the capacity to invade wild-type populations, potentially reaching and maintaining high infection frequencies. levels of virus transmission blocking are positively correlated with wolbachia intracellular density. despite reaching high densities in ae. aegypti, transinfections of walba, a strain native to aedes albopictus, showed ...201931120156
larvicidal activity of hypoestes forskaolii (vahl) r. br root extracts against anopheles gambiae giless.s, aedes aegypti l, and culex quinquefasciatussay.aim: this study aimed to evaluate larvicidal activity of hypoestes forskaolii r. br root extract against 3rd instar anopheles gambiae, aedes aegypti, and culex quinquefasciatus. methods: a protocol developed by the world health organization was adopted, with minor modification using chloroform and methanol extracts with concentrations ranging from 25-750 µg/ml. results: the h. forskaolii chloroform extract exhibited very high larvicidal activity after 72 hours of exposure, with lc50 2.0322, 3.89 ...201931118834
characterization of a broad-based mosquito yeast interfering rna larvicide with a conserved target site in mosquito semaphorin-1a genes.rna interference (rnai), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. saccharomyces cerevisiae (baker's yeast) may represent an effective interfering rna expression system that could be used directly for delivery of rna pesticides to mosquito larvae. here we describe characterization of a yeast larvicide developed through bioengineering ...201931118082
[experiences, barriers and facilitators to the implementation of interventions for controlling aedes aegypti in latin america and the caribbean: a qualitative study].this study sought to describe experiences, barriers and facilitators to the implementation of interventions for controlling aedes aegypti in latin america and the caribbean. a qualitative approach with semi-structured in-depth interviews with program implementation experts (19 participants in nine countries) was carried out. we used a software to codify the data and created matrices to compare them. based on our findings, we developed a graphic representation of the theoretical dimensions that e ...201931116251
aedes mosquito infestation in socioeconomically contrasting neighborhoods of panama city.the global expansion and proliferation of aedes aegypti and aedes albopictus represents a growing public health threat due to their capacity to transmit a variety of arboviruses to humans, including dengue, chikungunya, and zika. particularly important in urban regions, where these species have evolved to breed in man-made containers and feed nearly exclusively on human hosts, the threat of vector-borne disease has risen in recent decades due to the growth of cities, progression of climate chang ...201931114946
estimating absolute indoor density of aedes aegypti using removal sampling.quantification of adult aedes aegypti abundance indoors has relied on estimates of relative density (e.g. number of adults per unit of sampling or time), most commonly using traps or timed collections using aspirators. the lack of estimates of the sensitivity of collections and lack of a numerical association between relative and the absolute density of adult ae. aegypti represent a significant gap in vector surveillance. here, we describe the use of sequential removal sampling to estimate absol ...201931113454
use of mosquito repellents to protect against zika virus infection among pregnant women in brazil.to evaluate the use of repellents among pregnant women as a protective measure against infection with the zika virus.201931112836
predicting aquatic development and mortality rates of aedes aegypti.mosquito-borne pathogens continue to be a significant burden within human populations, with aedes aegypti continuing to spread dengue, chikungunya, and zika virus throughout the world. using data from a previously conducted study, a linear regression model was constructed to predict the aquatic development rates based on the average temperature, temperature fluctuation range, and larval density. additional experiments were conducted with different parameters of average temperature and larval den ...201931112566
the ion channel ppk301 controls freshwater egg-laying in the mosquito aedes aegypti.female aedes aegypti mosquitoes are deadly vectors of arboviral pathogens and breed in containers of freshwater associated with human habitation. because high salinity is lethal to offspring, correctly evaluating water purity is a crucial parenting decision. we found that the deg/enac channel ppk301 and sensory neurons expressing ppk301 control egg-laying initiation and choice in ae. aegypti. using calcium imaging, we found that ppk301-expressing cells show ppk301-dependent responses to water bu ...201931112133
juvenile hormone signaling in insect oogenesis.juvenile hormone (jh) plays a crucial role in insect reproduction, but its molecular mode of action only became clear within the last decade. we here review recent findings revealing the intricate crosstalk between jh and ecdysone signaling with nutrient sensing pathways in drosophila melanogaster, aedes aegypti, tribolium castaneum and locusta migratoria. the finding for a critical role of ecdysis triggering hormone (eth) in both molting and ooogenesis now also highlights the importance of an i ...201931109672
transmission potential of african, asian and american zika virus strains by aedes aegypti and culex quinquefasciatus from guadeloupe (french west indies).zika virus (zikv) is an arbovirus that has dramatically spread in south america and the caribbean regions since 2015. the majority of vector incrimination studies available for zikv showed that aedes aegypti mosquitoes are important vectors for this virus. however, several reports suggest that culex quinquefasciatus mosquitoes may be implicated in zikv transmission in certain urban settings. in the present study, we evaluated the vector competence for zikv of cx. quinquefasciatus and ae. aegypti ...201931109248
pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection.wolbachia's ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. wolbachia-infected aedes aegypti are currently being released in locations such as brazil, which regularly experience concurrent outbreaks of different arboviruses. a. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment.201931107912
tire shops in miami-dade county, florida are important producers of vector mosquitoes.human mobility in urban environments is a central part of urbanization and has determined the layout of how cities are projected, built and renovated. one of the most problematic issues of urbanization is how to properly dispose of used tires, considering the worldwide annual production of approximately 1.4 billion units every year. despite the efforts to properly dispose of used tires, they still represent a major problem for public health, notably serving as potential breeding sites for vector ...201931107881
characterization of a yeast interfering rna larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes.new mosquito control strategies are vitally needed to address established and emerging arthropod-borne infectious diseases. here we describe the characterization of a yeast interfering rna larvicide that was developed through the genetic engineering of saccharomyces cerevisiae (baker's yeast) to express a short hairpin rna targeting the aedes aegypti synaptotagmin (aae syt) gene. the larvicide effectively silences the aae syt gene, causes defects at the larval neural synapse, and induces high ra ...201931107878
neurological manifestations of pediatric arboviral infections in the americas.dengue, zika, chikungunya and yellow fever viruses are arboviruses transmitted by the mosquito aedes aegypti. these viruses exhibit marked neurotropism but have rarely been studied. here, we conduct an integrative review of the neurological manifestations caused by these arboviruses in the pediatric population. data on patients under 18 years of age were extracted from literature databases. the most frequently reported neurological manifestations were encephalitis, meningitis, seizures, hypotoni ...201931103802
induction of rna interference to block zika virus replication and transmission in the mosquito aedes aegypti.the yellow fever mosquito, aedes aegypti, serves as the primary vector for epidemic transmission of yellow fever, dengue, zika (zikv), and chikungunya viruses to humans. control of ae. aegypti is currently limited to insecticide applications and larval habitat management; however, to combat growing challenges with insecticide resistance, novel genetic approaches for vector population reduction or transmission interruption are being aggressively pursued. the objectives of this study were to asses ...201931103782
plant essential oils synergize various pyrethroid insecticides and antagonize malathion in aedes aegypti.pyrethroid resistance is a significant threat to agricultural, urban and public health pest control activities. because economic incentives for the production of novel active ingredients for the control of public health pests are lacking, this field is particularly affected by the potential failure of pyrethroid-based insecticides brought about by increasing pyrethroid resistance. as a result, innovative approaches are desperately needed to overcome insecticide resistance, particularly in mosqui ...201931102301
prevalence and distribution of dengue virus in aedes aegypti in yogyakarta city before deployment of wolbachia infected aedes aegypti.indonesia is one of the countries where dengue infection is prevalent. in this study we measure the prevalence and distribution of dengue virus (denv) denv-infected aedes aegypti in yogyakarta city, indonesia, during the wet season when high dengue transmission period occurred, as baseline data before implementation of a wolbachia-infected aedes aegypti trial for dengue control. we applied one-step multiplex real time pcr (rt-pcr) for the type-specific-detection of dengue viruses in field-caught ...201931100967
aedes aegypti saliva impairs m1-associated proinflammatory phenotype without promoting or affecting m2 polarization of murine macrophages.during the feeding process, the mouthparts of hematophagous mosquitoes break the skin barrier and probe the host tissue to find the blood. the saliva inoculated in this microenvironment modulates host hemostasis, inflammation and adaptive immune responses. however, the mechanisms involved in these biological activities remain poorly understood and few studies explored the potential roles of mosquito saliva on the individual cellular components of the immune system. here, we report the immunomodu ...201931097013
enzymatic and molecular characterization of insecticide resistance mechanisms in field populations of aedes aegypti from selangor, malaysia.dengue is a serious public health problem worldwide, including in selangor, malaysia. being an important vector of dengue virus, aedes aegypti are subjected to control measures which rely heavily on the usage of insecticides. evidently, insecticide resistance in ae. aegypti, which arise from several different point mutations within the voltage-gated sodium channel genes, has been documented in many countries. thus, this robust study was conducted in all nine districts of selangor to understand t ...201931097010
re-emergence of chikungunya in the republic of the congo in 2019 associated with a possible vector-host switch.in january 2019, an outbreak of chikungunya virus fever was reported in a rural region near pointe-noire, republic of the congo. molecular and phylogenetic analysis of this new chikv strain demonstrated the presence of the a226v substitution and a surprisingly close relation with aedes aegypti-associated central africa chikungunya strains. these results, combined with the preponderance of aedes albopictus in the outbreak area, suggest a recent vector-host switch facilitated by the emergence and ...201931096054
zika virus detection, isolation and genome sequencing through culicidae sampling during the epidemic in vitória, espírito santo, brazil.zika virus (zikv) has been isolated from many mosquito species in nature, but it is believed that the main vectors in urban environments are species of the genus aedes. here, we detected and isolated zikv in samples from aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus, collected during the zika epidemic in vitória, southeast brazil. using quantitative real-time polymerase chain reaction, zikv detection was performed in mosquito samples collected from february to april 2016.201931068218
determination of the spatial susceptibility to yellow fever using a multicriteria analysis.the outbreak of sylvatic yellow fever (syf) in humans during 2016-2017 in brazil is one of the greatest in the history of the disease. the occurrence of the disease in areas with low vaccination coverage favoured the dissemination of the disease; therefore, it is necessary to identify the areas vulnerability to the yf virus (yfv) to assist in the adoption of preventive measures.201931066755
embryonic development and egg viability of wmel-infected aedes aegypti.aedes aegypti is a major disease vector in urban habitats, involved in the transmission of dengue, chikungunya and zika. despite innumerous attempts to contain disease outbreaks, there are neither efficient vaccines nor definite vector control methods nowadays. in recent years, an innovative strategy to control arboviruses, which exploits the endosymbiotic bacterium wolbachia pipientis, emerged with great expectations. the success of the method depends on many aspects, including wolbachia's cyto ...201931060581
arboviral screening of invasive aedes species in northeastern turkey: west nile virus circulation and detection of insect-only viruses.the recent reports of aedes aegypti and ae. albopictus populations in turkey, in parallel with the territorial expansion identified in several surrounding countries, have raised concerns about the establishment and re-establishment of these invasive aedes mosquitoes in turkey. this cross-sectional study was performed to detect aedes aegypti and ae. albopictus in regions of recent incursions, and screen for viral pathogens known to be transmitted elsewhere by these species.201931059502
dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors.dengue virus is an important arbovirus infection which transmitted by the aedes female mosquitoes. the attempt to control and early detection of this infection is a global public health issue at present. because of the clinical importance of its detection, the main focus of this review is on all of the methods that can offer the new diagnosis strategies. the advantages and disadvantages of reported methods have been discussed comprehensively from different aspects like biomarkers type, sensitivi ...201931055654
evidence for infection but not transmission of zika virus by aedes albopictus (diptera: culicidae) from spain.a number of mosquito-borne viruses such as dengue virus (denv), usutu virus (usuv), west nile virus (wnv) are autochthonously transmitted in europe and six invasive mosquito species have been detected in this temperate region. this has increased the risk for the emergence of further mosquito-borne diseases. however, there is a paucity of information on whether european populations of invasive mosquito species are competent to transmit arboviruses. in this study, the susceptibility of aedes albop ...201931053164
an evaluation of efficacy of the auto-dissemination technique as a tool for aedes aegypti control in madeira, portugal.the frequency and intensity of arboviral epidemics is steadily increasing and posing an intractable public health burden. current vector control methods are proving ineffectual and despite progress in the development of high technology approaches, there is an urgent need for the development of tools for immediate implementation. several studies suggest that the auto-dissemination of pyriproxyfen (ppf) is a promising new approach to larviciding although there is little detail on the conditions un ...201931053095
a facile and rapid method for green synthesis of achyranthes aspera stem extract-mediated silver nano-composites with cidal potential against aedes aegypti l.aedes aegypti l. is the primary vector associated with transmission of globally concerned diseases; zika, yellow fever, dengue and chikungunya. present study investigates an efficient, alternative and comparative approach for mosquito control which is safe to environment and non-target organisms. the silver nano-composites (agncs) were synthesized from the aqueous stem extract of achyranthes aspera (aase) using different concentration of aqueous silver nitrate (agno3). the synthesis was tracked ...201931048994
voltages and resistances of the anterior malpighian tubule of drosophila melanogaster.the small size of malpighian tubules in the fruit fly drosophila melanogaster has discouraged measurements of the transepithelial electrical resistance. the present study introduces two methods for measuring the transepithelial resistance in isolated d. melanogaster malpighian tubules using conventional microelectrodes and pclamp hardware and software. the first method uses three microelectrodes to measure the specific transepithelial resistance normalized to tubule length or luminal surface are ...201931043456
similar neural pathways control foraging in mosquitoes and worms.female aedes aegypti mosquitoes bite human hosts to obtain a blood meal and, in the process, act as vectors for many disease-causing viruses, including the dengue, chikungunya, yellow fever, and zika viruses. after a complete meal, the female mosquitoes lose attraction to their hosts for several days. new research shows that pharmacological activation of neuropeptide y-like receptor (npylr) signaling elicits host aversion in female mosquitoes. this behavior of mosquitoes shows remarkable similar ...201931040241
resistance to temephos and deltamethrin in aedes aegypti from brazil between 1985 and 2017.aedes aegypti populations in brazil have been subjected to insecticide selection pressures with variable levels and sources since 1967. therefore, the brazilian ministry of health (moh) coordinated the activities of an ae. aegypti insecticide resistance monitoring network (morenaa) from 1999 to 2012.201931038548
using the basic reproduction number to assess the risk of transmission of lumpy skin disease virus by biting insects.in recent years, lumpy skin disease virus (lsdv) has emerged as a major threat to cattle outside africa, where it is endemic. although evidence suggests that lsdv is transmitted by the bites of blood sucking arthropods, few studies have assessed the risk of transmission posed by particular vector species. here this risk is assessed by calculating the basic reproduction number (r0 ) for transmission of lsdv by five species of biting insect: the stable fly, stomoxys calcitrans, the biting midge, c ...201931038286
aedes aegypti. 201931036346
exploring deeper genetic structures: aedes aegypti in brazil.aedes aegypti, being the principal vector of dengue (denv1 to 4), chikungunya and zika viruses, is considered as one of the most important mosquito vectors. in brazil, despite regular vector control programs, ae. aegypti still persists with high urban density in all the states. this study aimed to estimate the intra and inter population genetic diversity and genetic structure among 15 brazilian populations of ae. aegypti based on 12 microsatellite loci. a total of 510 specimens were analyzed com ...201931034798
distribution and relative densities of aedes aegypti in niger.its climate and environmental conditions put niger at particularly high risk of the spread of aedes mosquitoes, which can transmit arboviruses. in september 2016, the republic of niger reported its first outbreak of rift valley fever (rvf) in the northern region of tahoua, near the mali border, particularly in the departments of tchintabaraden, tassara, and abalak. the history of rvf has showed that epidemics and epizootics have a considerable socioeconomic impact in affected countries, such as ...201931031247
aedes aegypti mosquitoes use their legs to sense deet on contact.deet (n, n-diethyl-meta-toluamide) is the most effective and widely used insect repellent, but its mechanism of action is both complex and controversial [1]. deet acts on insect smell [2-6] and taste [7-11], and its olfactory mode of action requires the odorant co-receptor orco [2, 3, 6]. we previously observed that orco mutant female aedes aegypti mosquitoes are strongly attracted to humans even in the presence of deet, but they are rapidly repelled after contacting deet-treated skin [6]. deet ...201931031114
resistance status to deltamethrin, permethrin, and temephos along with preliminary resistance mechanism in aedes aegypti (diptera: culicidae) from punjab, pakistan.the use of insecticides such as deltamethrin, permethrin, and temephos has been a primary tool to manage aedes aegypti (linnaeus) in punjab province, pakistan; however, recent reports of control failure necessitate monitoring insecticide resistance. for this reason, we evaluated 12 field strains of ae. aegypti from punjab for resistance against deltamethrin, permethrin, and temephos along with underlying resistance mechanisms. for deltamethrin, high level of resistance was observed in rawalpindi ...201931028391
association of age, sex, and pyrethroid resistance status on survival and cytochrome p450 gene expression in aedes aegypti (l.).aedes aegypti is a vector of viruses that negatively impact human health. insecticide resistance complicates mosquito control efforts, but understanding the mechanisms of resistance can help to improve management practices. this study examined different factors that could influence the interpretation of toxicity bioassays and gene expression studies in a. aegypti, including sex and age, in the context of resistance to pyrethroids. bioassays using a pyrethroid-resistant strain, puerto rico (pr), ...201931027587
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