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impact of mosquito age and insecticide exposure on susceptibility of aedes albopictus (diptera: culicidae) to infection with zika virus.zika virus (zikv) is primarily transmitted to humans by aedes aegypti and ae. albopictus. vector⁻virus interactions influencing vector competence vary and depend on biological and environmental factors. a mosquito's chronological age may impact its immune response against virus infection. insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit zikv. this study assessed the effects of a mosquito's chronological age at time of infecti ...201830103526
susceptibility and vectorial capacity of american aedes albopictus and aedes aegypti (diptera: culicidae) to american zika virus strains.the rapid expansion of zika virus (zikv), following the recent outbreaks of chikungunya virus, overwhelmed the public health infrastructure in many countries and alarmed many in the scientific community. aedes aegypti (l.) (diptera: culicidae) and aedes albopictus (skuse) (diptera: culicidae) have previously been incriminated as the vectors of these pathogens in addition to dengue virus. in our study, we challenged low generation ae. aegypti (chiapas, mexico) and ae. albopictus (north carolina, ...201930102327
optimization of cationic (q)-paper for detection of arboviruses in infected mosquitoes.previously (glushakova et al. 2017), a cellulose-based cationic (q) paper derivatized with quaternary ammonium groups was shown to be a convenient platform to collect, preserve, and store nucleic acids (nas) derived from mosquito vectors infected with pathogens for surveillance. nas bind electrostatically to q-paper, but the quantity of na bound depends on the paper's binding capacity. to optimize the original technology for mosquito surveillance, factors that affected na absorbance on q-paper w ...201830099053
discovery and high prevalence of phasi charoen-like virus in field-captured aedes aegypti in south china.arboviruses have caused significant global health concerns during the past decade. in this regard, continuous viral surveillance is essential to timely identify emerging arboviruses and other novel viruses. here, a novel isolate of phasi charoen-like virus (pclv zhanjiang01) was identified from field-captured aedes aegypti mosquitoes in zhanjiang by next generation sequencing. phylogenetic analysis suggested that pclv zhanjiang01 belonged to the genus phasivirus in the family phenuiviridae. the ...201830077072
managing aedes aegypti populations in the first zika transmission zones in the continental united states.the african zika virus swept across the pacific, reaching the new world in 2014. in july, 2016, miami-dade county, florida became the locus of the first mosquito-borne zika transmission zones in the continental united states. control efforts were guided by the centers for disease control and prevention, including aerial and truck sprays of adulticides and larvicides. to improve our understanding of how best to fight zika transmission in an urban environment in the developed world, trap counts of ...201830075097
genetic characterization of chikungunya viruses isolated during the 2015-2017 outbreaks in different states of india, based on their e1 and e2 genes.during 2015-2017, chikungunya virus (chikv) showed a resurgence in several parts of india with karnataka, maharashtra and new delhi accounting for a majority of the cases. e2-e1 gene based characterization revealed indian subcontinent sublineage strains possessing aedes aegypti mosquito-adaptive mutations e1: k211e and e2:v264a, with the 211 site positively selected. novel mutational sites e1: k16e/q, e1: k132q/t, e1: s355t, e2: c19r and e2:s185y could be associated with epitopes or virulence de ...201830073419
a low-cost, battery-powered acoustic trap for surveilling male aedes aegypti during rear-and-release operations.the aedes aegypti mosquito is a primary vector of several serious arboviruses throughout the world and is therefore of great concern to many public health organizations. with vector control methodology pivoting towards rearing and releasing large numbers of genetically modified, sterilized, or wolbachia-infected male mosquitoes to control vector populations, economical surveillance methods for release tracking becomes increasingly necessary. previous work has identified that male ae. aegypti are ...201830071091
rapid evolution and the genomic consequences of selection against interspecific mating.while few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. aedes albopictus (the asian tiger mosquito) was introduced into the naturalized range of aedes aegypti (the yellow fever mosquito) in the united states in the mid-1980s, resulting in the displacement of a. aegypti in much of the south-eastern united states. the rapid displacem ...201830069966
small females prefer small males: size assortative mating in aedes aegypti mosquitoes.with aedes aegypti mosquitoes now being released in field programmes aimed at disease suppression, there is interest in identifying factors influencing the mating and invasion success of released mosquitoes. one factor that can increase release success is size: released males may benefit competitively from being larger than their field counterparts. however, there could be a risk in releasing only large males if small field females avoid these males and instead prefer small males. here we invest ...201830068363
a gustatory receptor used for rapid detection of tyrophagus putrescentiae in fungi hosts.the storage mite, tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in china. storage mites are tiny and difficult to observe, especially when they occur in fungi composts. in this study, one gustatory receptor protein (tputgr1) was identified from the transcriptome of t. putrescentiae. phylogenetic analysis of grs families from 10 arthropod species revealed that tputgr1 had high homology with the sccagr1 of sarcoptes scabiei and turtgr1-2 of tetran ...201830061635
juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes.juvenile hormone (jh) regulates many aspects of insect development and reproduction. in some processes, jh plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20e). in aedes aegypti mosquitoes, jh prepares newly emerged female adults to become competent to synthesize vitellogenin in response to 20e after blood ingestion. the molecular basis of this competence is still not well understood. here, we report that jh regulates pre-mrna splicing of the taiman gene, ...201830061397
comparing the effectiveness of different strains of wolbachia for controlling chikungunya, dengue fever, and zika.once aedes aegypti and aedes albopictus mosquitoes that spread chikungunya virus, dengue virus, and zika virus are infected with wolbachia, they have reduced egg laying rates, reduced transmission abilities, and shorter lifespans. since most infected mosquitoes are only infectious in the last few days of their lives, shortening a mosquito's lifespan by a day or two can greatly reduce their abilities to spread mosquito-borne viral diseases, such as chikungunya, dengue fever, and zika. we develope ...201830059498
modeling the importation and local transmission of vector-borne diseases in florida: the case of zika outbreak in 2016.chikungunya, dengue, and zika viruses are all transmitted by aedes aegypti and aedes albopictus mosquito species, had been imported to florida and caused local outbreaks. we propose a deterministic model to study the importation and local transmission of these mosquito-borne diseases. the purpose is to model and mimic the importation of these viruses to florida via travelers, local infections in domestic mosquitoes by imported travelers, and finally non-travel related transmissions to local huma ...201830053386
modifying the biogents sentinel trap to increase the longevity of captured aedes aegypti.aedes aegypti (l.) (diptera: culicidae) is a vector of viruses causing dengue, zika, chikungunya, and yellow fever and subsequently pose a significant global threat to public health. while sampling live mosquitoes is useful for surveillance purposes, most traps targeting aedes kill captured mosquitoes. the biogents sentinel (bgs) trap, the gold standard for capturing ae. aegypti, is one such trap. in our study, we modified the bgs trap to increase the survival of captured ae. aegypti by replacin ...201830053026
experimental study of the susceptibility of a european aedes albopictus strain to dengue virus under a simulated mediterranean temperature regime.dengue virus (denv) has re-emerged in europe driven by the geographic expansion of the mosquito species aedes albopictus and aedes aegypti (diptera: culicidae) and the introduction of the virus by viraemic travellers. in the present study, the vector competence (vc) of ae. albopictus collected in catalonia (northeast spain) was evaluated for two different denv strains, denv-1 and denv-2, the serotypes responsible for all outbreaks of dengue that have occurred in europe. mosquitoes were reared un ...201830051490
larvicidal effectiveness of azolla pinnata against aedes aegypti (diptera: culicidae) with its effects on larval morphology and visualization of behavioural response.dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. aedes aegypti (l.) (diptera: culicidae) is a primary vector for dengue viral infections for humans. current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. the extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and ...201830050688
the impact of the age of first blood meal and zika virus infection on aedes aegypti egg production and longevity.the impact of senescence and pathogen infection on aedes aegypti life-history traits remains poorly understood. this laboratory study focused on the impact of zika virus (zikv) infection and the age of first blood intake on blood meal and clutch sizes, and more importantly on the egg production ratio per μl of blood. three groups of zikv-infected and uninfected ae. aegypti females that received their first blood meal at 7 (young feeders), 14 (mature feeders) and 21 days old (old feeders) were mo ...201830048481
characterization of dengue virus in aedes aegypti and aedes albopictus spp. of mosquitoes: a study in khyber pakhtunkhwa, pakistan.dengue is a vector-borne disease caused by dengue virus. according to the recent report of cdc that one-third population of the world are at high risk with dengue fever. the prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. aedes mosquitoes was reported as the main cause of transmission of dengue virus. so the current study was planned to characterize the virus in aedes mosquitoes collected from different area of pakistan. in current inv ...201830046621
specific human antibody responses to aedes aegypti and aedes polynesiensis saliva: a new epidemiological tool to assess human exposure to disease vectors in the pacific.aedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. in french polynesia, aedes aegypti is the main vector of dengue, chikungunya and zika, and aedes polynesiensis the primary vector of bancroftian filariasis and a secondary vector of arboviruses. tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. a promising approach to quantify the human-vector contact relies ...201830040826
is autumn the key for dengue epidemics in non endemic regions? the case of argentina.dengue is a major and rapidly increasing public health problem. in argentina, the southern extreme of its distribution in the americas, epidemic transmission takes place during the warm season. since its re-emergence in 1998 two major outbreaks have occurred, the biggest during 2016. to identify the environmental factors that trigger epidemic events, we analyzed the occurrence and magnitude of dengue outbreaks in time and space at different scales in association with climatic, geographic and dem ...201830038860
the effect of skitosnack, an artificial blood meal replacement, on aedes aegypti life history traits and gut microbiota.public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. all target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. vertebrate blood, however, is costly, with a short shelf life. to overcome these constraints, we have developed skitosnack, an artificial blood meal replacement for the mosquito aedes aegypti, the vector of dengue, zika and chikungunya virus. skitosnack contains bovine ...201830038361
classification of containers with aedes aegypti pupae using a neural networks model.this paper discusses the presence of aedes aegypti pupae in different types of containers considering: volume, ph of the container, among other variables.201830036370
acute inhalation toxicity study of n-heneicosane and its combination with diflubenzuron: an attracticide of aedes aegypti.combination of an oviposition pheromone and an insect growth regulator for the control of vectors is an effective approach. there is a need for toxicological evaluation before its introduction. the present study evaluates the acute inhalation toxicity of n-heneicosane and its combination with diflubenzuron in a head-only inhalation exposure chamber made of glass.201830033812
cgti, a novel thermostable kunitz trypsin-inhibitor purified from cassia grandis seeds: purification, characterization and termiticidal activity.cassia grandis trypsin inhibitor (cgti) is a novel plant serine proteinase inhibitor. this study sets out to purify a thermostable inhibitor from the seeds of cassia grandis and to provide biochemical information about a novel peptide belonging to the kunitz family. moreover, toxicity assays against artemia, aedes aegypti larvae-l4 and nasutitermes corniger are evaluated. the purification process was performed using acetone precipitation, trypsin-sepharose-cl4b and superdex-g75. the inhibitor sh ...201830030073
pan-american league of associations for rheumatology-central american, caribbean and andean rheumatology association consensus-conference endorsements and recommendations on the diagnosis and treatment of chikungunya-related inflammatory arthropathies in latin america.although mortality rates related with chikungunya (chik) outbreaks in latin america's (la's) dengue-endemic rural and new urban regions are low, dealing with symptoms and sequelae can both produce a significant burden of disease and diminish quality of life-from many months to years-after the acute phase of the infection, with a significant impact on public and individual health.the aim of this work was to establish pan-american league of associations for rheumatology-central american, caribbean ...201930028809
modelling the distribution of the vector aedes aegypti in a central argentine city.aedes aegypti (diptera: culicidae) is an urban mosquito involved in the transmission of numerous viruses, including dengue, chikungunya and zika. in argentina, ae. aegypti is the main vector of dengue virus and has been involved in several outbreaks in regions ranging from northern to central argentina since 2009. in order to evaluate areas of potential vector-borne disease transmission in the city of córdoba, argentina, the present study aimed to identify the environmental, socioeconomic and de ...201830027565
assessment of insecticide resistance in primary dengue vector, aedes aegypti (linn.) from northern districts of west bengal, india.aedes mosquitoes are the major vectors transmitting several arboviral diseases such as dengue, zika and chikungunya worldwide. northern districts of west bengal is home to several epidemics vectored by mosquito including dengue infections, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches. this study was carried out to unveil the level of insecticide resistance prevailing among the primary ...201830026024
copy number variation (cnv) and insecticide resistance in mosquitoes: evolving knowledge or an evolving problem?copy number variation (cnv) in insect genomes is a rich source of potentially adaptive polymorphism which may help overcome the constraints of purifying selection on conserved genes and/or permit elevated transcription. classic studies of amplified esterases and acetylcholinesterase duplication in culex pipiens quantified evolutionary dynamics of cnv driven by insecticidal selection. a more complex and potentially medically impactful form of cnv is found in anopheles gambiae, with both heterogen ...201830025639
melanization of dirofilaria immitis larvae in different culicid species.dirofilaria immitis is an important filarioid transmitted by culicids. the vector role of these arthropods may be influenced by biological events as melanization against d. immitis larvae. this study aimed to detect the occurrence of melanization in culex quinquefasciatus, aedes (stegomyia) aegypti and aedes (stegomyia) albopictus experimentally infected with d. immitis.201830018997
the use of insecticide-treated curtains for control of aedes aegypti and dengue virus transmission in "fraccionamiento" style houses in méxico.dengue, chikungunya, yellow fever, and zika viruses transmitted by aedes aegypti mosquitoes are major public health threats in the tropical and subtropical world. in méxico, construction of large tracts of "fraccionamientos" high density housing to accommodate population growth and urbanization has provided fertile ground for ae. aegypti-transmitted viruses. we investigated the utility of pyrethroid-treated window curtains to reduce both the abundance of ae. aegypti and to prevent dengue virus ( ...201830018645
zika virus outbreak in the pacific: vector competence of regional vectors.in 2013, zika virus (zikv) emerged in french polynesia and spread through the pacific region between 2013 and 2017. several potential aedes mosquitoes may have contributed to the zikv transmission including aedes aegypti, the main arbovirus vector in the region, and aedes polynesiensis, vector of lymphatic filariasis and secondary vector of dengue virus. the aim of this study was to analyze the ability of these two pacific vectors to transmit zikv at a regional scale, through the evaluation and ...201830016372
molecular characterization of the cry gene profile of bacillus thuringiensis isolated from a caribbean region of colombia.in order to characterize native strains of bacillus thuringiensis of the colombian caribbean with toxic effect against insect vectors, 28 samples of bacteria identified as b. thuringiensis were isolated from different soils and muds around the city of valledupar. using a biological test, five isolates of b. thuringiensis showed toxic effect against larvae of aedes aegypti. pcr methods were used to detect cry1, cry2, cry4b, cry10 and cyt1 genes. cry1 and cry2 genes were detected in 35.7% and 32.1 ...201830015421
evidence of vertical transmission of zika virus in field-collected eggs of aedes aegypti in the brazilian amazon.arboviruses are viruses transmitted to humans and other animals by the bite of hematophagous arthropods. infections caused by chikungunya virus (chikv), dengue virus (denv), zika virus (zikv), and the deadlier yellow fever virus (yfv) are current public health problems in several countries, mainly those located in tropical and subtropical regions. one of the main prevention strategies continues to be vector control, with the elimination of breeding sites and surveillance of infested areas. the u ...201830011278
transmission potential of mayaro virus in florida aedes aegypti and aedes albopictus mosquitoes.mayaro virus (mayv) is an emerging mosquito-borne arbovirus present in central and south america that causes arthralgia and febrile illness. domestic mosquitoes aedes aegypti (diptera: culicidae) and aedes albopictus are potential vectors of mayv that may allow for transmission to humans in urban settings. the present paper assesses susceptibility to infection, disseminated infection and transmission potential in florida ae. aegypti and ae. albopictus for mayv. oral infection was significantly h ...201830006976
the occurrence, diversity and blood feeding patterns of potential vectors of dengue and yellow fever in kacheliba, west pokot county, kenya.yellow fever (yf) and dengue (den) viruses are important re-emerging mosquito-borne viruses sharing similar vectors and reservoirs. the last documented yf outbreak in kenya occurred in 1992-95. however, yf virus is re-emerging in bordering countries including uganda, ethiopia and south sudan with the potential for spread to the neighboring regions in kenya. dengue is endemic in kenya with outbreaks being detected in various towns in the north and the coast. this study reports on the aedes (stego ...201830006028
synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against aedes aegypti l. (diptera: culicidae).in a previous screening program for mosquitocides from local edible plants in thailand, essential oils (eos) of cyperus rotundus, alpinia galanga and cinnamomum verum, were found to possess promising adulticidal activity against aedes aegypti. with the aim of reducing usage of conventional insecticides and improving the management of resistant mosquito populations, this study was designed to determine the potential synergism in the adulticidal efficacy of eos on permethrin toxicity against ae. a ...201830005688
epidemiological significance of dengue virus genetic variation in mosquito infection dynamics.the kinetics of arthropod-borne virus (arbovirus) transmission by their vectors have long been recognized as a powerful determinant of arbovirus epidemiology. the time interval between virus acquisition and transmission by the vector, termed extrinsic incubation period (eip), combines with vector mortality rate and vector competence to determine the proportion of infected vectors that eventually become infectious. however, the dynamic nature of this process, and the amount of natural variation i ...201830005085
the c-type lectin domain gene family in aedes aegypti and their role in arbovirus infection.several medically important flaviviruses that are transmitted by mosquitoes have been shown to bind to the c-type lectin fold that is present in either vertebrate or invertebrate proteins. while in some cases this interaction is part of a neutralizing anti-viral immune response, many reports have implicated this as critical for successful virus entry. despite the establishment of mosquito c-type lectin domain containing proteins (ctldcps) as known host factors in assisting the infectious process ...201830002303
lippia gracilis essential oil in β-cyclodextrin inclusion complexes: an environmentally safe formulation to control aedes aegypti larvae.one of the most efficient ways to prevent arboviruses, such as dengue fever, yellow fever, chikungunya and zika, is by controlling their vector, the aedes aegypti. because this vector is becoming resistant to most larvicides used, the development of new larvicides should be considered. β-cyclodextrin (β-cd) complexes have been investigated as an interesting way of enabling the use of essential oils in water as larvicides. this study comprised the development of lippia gracilis essential oil (lge ...201929998608
identification of aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects.sophisticated tools for manipulation of gene expression in select neurons, including neurons that regulate sexually dimorphic behaviors, are increasingly available for analysis of genetic model organisms. however, we lack comparable genetic tools for analysis of non-model organisms, including aedes aegypti, a vector mosquito which displays sexually dimorphic behaviors that contribute to pathogen transmission. formaldehyde-assisted isolation of regulatory elements followed by sequencing (faire-se ...201829996889
tropomyosins in mosquito and house dust mite cross-react at the humoral and cellular level.aedes aegypti and dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. however, the functional and clinical relevance of their cross-reactivity is still debated.201829992665
mosquito arbovirus survey in selected areas of kenya: detection of insect-specific virus.many arboviral outbreaks have occurred in various locations in kenya. entomological surveys are suitable methods for revealing information about circulating arboviruses before human outbreaks are recognized. therefore, mosquitoes were collected in kenya to determine the distribution of arboviruses.201829991925
repellent and insecticidal efficacy of a combination of dinotefuran, pyriproxyfen and permethrin (vectra® 3d) against culex pipiens in dogs.culex pipiens is an important vector of pathogens of substantial medical and veterinary importance such as dirofilaria immitis and dirofilaria repens or the west nile virus. the control of these mosquitoes is therefore essential to control the transmission of mosquito-borne agents to humans and animals. a combination of dinotefuran, permethrin and pyriproxyfen (vectra® 3d) has already shown its efficacy against aedes aegypti. the aim of this study was to confirm the efficacy of this combination ...201629988200
bioefficacy of ecbolin a and ecbolin b isolated from ecbolium viride (forsk.) alston on dengue vector aedes aegypti l. (diptera: culicidae).ecbolin a and ecbolin b were isolated from ethyl acetate extract of ecbolium viride (forsk.) alston root and evaluated for larvicidal and growth disturbance activities against aedes aegypti l. (diptera: culicidae). for larvicidal activity, the third instar larvae of a. aegypti were exposed to different concentrations viz., 1.0, 2.5, 5.0 and 10 ppm for each compound. among the two compounds screened, ecbolin b recorded highest larvicidal activity with lc50 and lc90 values of 0.70 and 1.42 ppm, re ...201629988190
effect of gamma radiation on life history traits of aedes aegypti (l.).aedes aegypti is an important vector for dengue and dengue hemorrhagic fever. considering its medical importance and its relevance as a model system, this study was undertaken to evaluate the impact of different doses of gamma radiation for three generations of a. aegypti. two to three days old virgin males of a. aegypti were irradiated with 15 doses of gamma radiation, ranging from 1 to 50 gy and were immediately mass mated with the same aged virgin females. observations were made for changes o ...201629988174
environmentally-friendly green approach for the production of zinc oxide nanoparticles and their anti-fungal, ovicidal, and larvicidal properties.green synthesis of nanoparticles can be an important alternative compared to conventional physio-chemical synthesis. we utilized scadoxus multiflorus leaf powder aqueous extract as a capping and stabilizing agent for the synthesis of pure zinc oxide nanoparticles (zno nps). further, the synthesized zno nps were subjected to various characterization techniques. transmission electron microscope (tem) analysis showed an irregular spherical shape, with an average particle size of 31 ± 2 nm. furtherm ...201829986435
molecular aspects of the dengue virus infection process: a review.at the present time, dengue is one of the most important arboviruses affecting man, becoming a serious global public health problem, especially in subtropical and tropical countries, where environmental conditions favor the development and proliferation of the mosquito aedes aegypti. dengue is caused by a type of flavivírus, which is an enveloped virus of spherical geometry. nowadays, it is one of the diseases with the highest incidence in brazil, reaching the population of all states, regardles ...201829984641
aedes aegypti in the black sea: recent introduction or ancient remnant?the yellow fever mosquito aedes aegypti transmits viral diseases that have plagued humans for centuries. its ancestral home are forests of africa and ~400-600 years ago it invaded the new world and later europe and asia, causing some of the largest epidemics in human history. the species was rarely detected in countries surrounding the mediterranean sea after the 1950s, but during the last 16 years it re-appeared in madeira, russia and in the eastern coast of the black sea. we genotyped ae. aegy ...201829980229
west nile virus and other mosquito-borne viruses present in eastern europe.eastern europe (ee) has been severely affected by mosquito-borne viruses (moboviruses). in this review, we summarize the epidemiology of moboviruses, with particular attention to west nile virus (wnv). the study of wnv human cases in ee between 2010 and 2016, revealed that the epidemiology of wnv in ee is complex with the combination of introduction of different wnv strains from lineages 1 and 2, and the establishment of endemic cycles. we found a positive correlation between the risk of wnv re- ...201829979950
both living bacteria and eukaryotes in the mosquito gut promote growth of larvae.we recently reported that larval stage aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. we further reported that living bacteria induce a hypoxia signal in the gut, which activates hypoxia-induced transcription factors and other processes larvae require for growth. in this study we assessed whether other types of organisms induce mosquito larvae to grow and asked if the density of non-living mi ...201829979680
[construction of aaldv-3 mediated mirrna sponge delivery system and its effect on cell and larva of aedes aegypti].micrornas (mirnas) not only play the key roles in regulation of the growth and development of mosquito, but also has an important function in interaction between the pathogen and vector. so, mirnas can be used as the molecular target for development of an alternative method for mosquito and mosquito-borne disease control. however, an effective delivery system is still need. mosquito densovirus have the potential for vector control as transducing agents to express foreign toxins or small interfer ...201629979549
variation in temephos resistance in field populations of aedes aegypti (diptera: culicidae) in the state of sergipe, northeast brazil.the organophosphate temephos was first used in brazil in the 1960s for the control of aedes aegypti. because of its extensive and longstanding use worldwide, selection for mosquito populations resistant to the chemical has been observed not only in different regions of brazil but also in several parts of the world. the objective of this study was to evaluate the susceptibility of ae. aegypti to the organophosphate temephos, a larvicide used in vector control activities in sergipe/northeast brazi ...201829972557
zika virus: - a review of the main aspects of this type of arbovirosis.the objective of this study was to integrate findings related to zika virus from the scientific literature. an integrative review of medline was conducted, and data search was performed using the health sciences descriptor zika virus and the following filters: full texts available; studies in humans; full articles; and publications in portuguese, french, english, and spanish. after application of the filters, followed by reading of the titles, abstracts, and full texts, 44 studies were included ...201829972554
encapsulation of piper aduncum and piper hispidinervum essential oils in gelatin nanoparticles: a possible sustainable control tool of aedes aegypti, tetranychus urticae and cerataphis lataniae.the encapsulated essential oils (eos) of piper aduncum l. and piper hispidinervum c. dc. in gelatin nanoparticles were evaluated against aedes aegypti linn., tetranychus urticae koch and cerataphis lataniae boisd.201929971785
the effect of nonrandom mating on wolbachia dynamics: implications for population replacement and sterile releases in aedes mosquitoes.wolbachia bacteria are known to cause deviations from random mating and affect sperm competition (sc) in some of their arthropod hosts. because these effects could influence the effectiveness of wolbachia in mosquito population replacement and suppression programs, we developed a theoretical framework to investigate them and we collected relevant data for the wmel infection in aedes aegypti. using incompatibility patterns as a measure of mating success of infected versus uninfected mosquitoes, w ...201829968550
strain-specific pathogenicity and subversion of phenoloxidase activity in the mosquito aedes aegypti by members of the fungal entomopathogenic genus isaria.development of alternative vector control strategies are becoming more pressing given the rapid evolution of insecticide resistance and the rise of vector borne pathogens affecting public health such as dengue, chikungunya and zika. fungal-based biopesticides are promising alternatives to synthetic insecticides because they are ecofriendly and are highly effective at infecting insects through contact. this study evaluated the susceptibility of the yellow fever mosquito ae. aegypti to a range of ...201829967469
continued susceptibility of the wmel wolbachia infection in aedes aegypti to heat stress following field deployment and selection.aedes aegypti mosquitoes infected with the wmel strain of wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. blockage by wolbachia in some scenarios may be affected by the susceptibility of wmel to cyclical heat stress during mosquito larval development. we therefore evaluated the potential to generate a heat-resistant strain of wmel in ae. aegypti through artificial laboratory selection and through exposure to field tempera ...201829966368
variation in competence for zikv transmission by aedes aegypti and aedes albopictus in mexico.zikv is a new addition to the arboviruses circulating in the new world, with more than 1 million cases since its introduction in 2015. a growing number of studies have reported vector competence (vc) of aedes mosquitoes from several areas of the world for zikv transmission. some studies have used new world mosquitoes from disparate regions and concluded that these have a variable but relatively low competence for the asian lineage of zikv.201829965958
chikungunya in children: a clinical review.chikungunya (chikv) is an emerging arboviral infection with recent spikes in transmission in the americas. chikungunya is most commonly transmitted by mosquitos, specifically aedes aegypti and aedes albopictus. these mosquitoes are found throughout many parts of the united states. the classic tetrad of symptoms for chikv is fever, symmetric polyarthralgia, maculopapular rash, and nonpurulent conjunctivitis. although the majority (3 of 4) of infected people will be symptomatic, the viral illness ...201829965819
adventitious viruses persistently infect three commonly used mosquito cell lines.mosquito cell lines have been used extensively in research to isolate and propagate arthropod-borne viruses and understand virus-vector interactions. despite their utility as an in vitro tool, these cell lines are poorly defined and may harbor insect-specific viruses. accordingly, we screened four commonly-used mosquito cell lines, c6/36 and u4.4 cells from aedes albopictus, aag2 cells from aedes aegypti, and hsu cells from culex quinquefasciatus, for the presence of adventitious (i.e. exogenous ...201829957338
patterns of occurrence of dengue and chikungunya, and spatial distribution of mosquito vector aedes albopictus in swabi district, pakistan.two principal vector species, aedes aegypti and aedes albopictus, are known for transmission of dengue (den) and chikungunya (chk) in pakistan. we aimed to investigate their spatial and temporal distribution.201829956428
empirical optimization of risk thresholds for dengue: an approach towards entomological management of aedes mosquitoes based on larval indices in the kandy district of sri lanka.larval indices such as premise index (pi), breteau index (bi) and container index (ci) are widely used to interpret the density of dengue vectors in surveillance programmes. these indices may be useful for forecasting disease outbreaks in an area. however, use of the values of these indices as alarm signals is rarely considered in control programmes. therefore, the current study aims to propose threshold values for vector indices based on an empirical modeling approach for the kandy district of ...201829954443
comparison of chikungunya viruses generated using infectious clone or the infectious subgenomic amplicons (isa) method in aedes mosquitoes.reverse genetics systems provide the opportunity to manipulate viral genomes and have been widely used to study rna viruses and to develop new antiviral compounds and vaccine strategies. the recently described method called isa (infectious subgenomic amplicons) gives the possibility to rescue rna viruses in days. we demonstrated in cell culture that the use of the isa method led to a higher genetic diversity of viral populations than that observed using infectious clone technology. however, no r ...201829953474
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952164
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952163
aedes anphevirus: an insect-specific virus distributed worldwide in aedes aegypti mosquitoes that has complex interplays with wolbachia and dengue virus infection in cells.insect-specific viruses (isvs) of the yellow fever mosquito aedes aegypti have been demonstrated to modulate transmission of arboviruses such as dengue virus (denv) and west nile virus by the mosquito. the diversity and composition of the virome of a. aegypti, however, remains poorly understood. in this study, we characterized aedes anphevirus (aeav), a negative-sense rna virus from the order mononegavirales aeav identified from aedes cell lines was infectious to both a. aegypti and aedes albopi ...201829950416
arthropod evs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein tsp29fb.dengue virus (denv) is a mosquito-borne flavivirus that causes dengue fever in humans, worldwide. using in vitro cell lines derived from aedes albopictus and aedes aegypti, the primary vectors of denv, we report that denv2/denv3-infected cells secrete extracellular vesicles (evs), including exosomes, containing infectious viral rna and proteins. a full-length denv2 genome, detected in arthropod evs, was infectious to naïve mosquito and mammalian cells, including human-skin keratinocytes and bloo ...201829946031
model-based analysis of experimental data from interconnected, row-configured huts elucidates multifaceted effects of a volatile chemical on aedes aegypti mosquitoes.insecticides used against aedes aegypti and other disease vectors can elicit a multitude of dose-dependent effects on behavioral and bionomic traits. estimating the potential epidemiological impact of a product requires thorough understanding of these effects and their interplay at different dosages. volatile spatial repellent (sr) products come with an additional layer of complexity due to the potential for altered movement of affected mosquitoes and diffusion of volatile particles of the produ ...201829941038
wolbachia enhances insect-specific flavivirus infection in aedes aegypti mosquitoes.mosquitoes transmit a diverse group of human flaviviruses including west nile, dengue, yellow fever, and zika viruses. mosquitoes are also naturally infected with insect-specific flaviviruses (isfs), a subgroup of the family not capable of infecting vertebrates. although isfs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits t ...201829938064
using barcoded zika virus to assess virus population structure in vitro and in aedes aegypti mosquitoes.arboviruses such as zika virus (zikv, flaviviridae; flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity may be significantly reduced. to help quantify these bottlenecks and their effects, we constructed 4 "barcoded" zikv populations that theoretically contain thousands of barcodes each. after identifying ...201829935423
circulation of chikungunya virus in aedes aegypti in maranhão, northeast brazil.the simultaneous circulation of dengue virus (denv), chikungunya virus (chikv) and zika virus (zikv) arboviruses have placed brazil among the main worldwide endemic areas. brazilian northeast region concentrates the highest incidence of infections caused by chikv and zikv. in maranhão, the second biggest northeastern state, there are cases of human infections caused by these three arboviruses and presence of aedes aegypti and aedes albopictus vectors. in this context, this study aimed to investi ...201829932932
ancient treatment for lice: a source of suggestions for carriers of other infectious diseases?louse infestation is one of the oldest contagious pestilential diseases of humankind, which has recently re-emerged in several developing countries as well as in homeless individuals and migrants. the present work provides the first phase of an historical excursus of louse remedies based on the classics of pharmaceutical literature, codes, pharmacopoeia and treatises. the second phase involves a literature search, based on the principal medical databases (scifinder, pubmed, google scholar, isi-w ...201829932096
evaluation of aedes aegypti (diptera: culicidae) life table attributes upon chikungunya virus replication reveals impact on egg-laying pathways.arthropod-borne virus disease cycles constitute interactions among three primary players-the host, the vector, and the virus-in which the virus needs to interact with the host and the vector to establish its survival. while in the host, some arboviruses replicate aggressively, resulting in host pathogenicity, and manifest as a disease condition. these viruses more often utilize the vectors as reservoirs before they are transmitted to the host and therefore do not amplify to as large titers as th ...201829931258
a high-risk zika and dengue transmission hub: virus detections in mosquitoes at a brazilian university campus.zika virus (zikv) and dengue virus (denv) are mosquito-borne flaviviruses prevalent throughout tropical regions. currently, management of zikv and denv centers on control of the primary vector aedes aegypti. this vector is highly anthropophilic and is therefore prevalent throughout densely urbanised landscapes. a new passive trap for gravid ae. aegypti (gravid aedes trap - gat) was developed for mosquito surveillance. here the different killing agents and the level of transmission of arboviruses ...201829929561
risk factors for the presence of dengue vector mosquitoes, and determinants of their prevalence and larval site selection in dhaka, bangladesh.dengue viruses are responsible for over 100 million infections a year worldwide and are a public health concern in bangladesh. although risk of transmission is high, data on vector population characteristics are scanty in bangladesh; therefore, a comprehensive prediction of the patterns of local virus transmission is not possible. recognizing these gaps, multi-year entomological surveys were carried out in dhaka, where the disease is most frequently reported. the specific objectives of the prese ...201829928055
vector competence of aedes aegypti, culex tarsalis, and culex quinquefasciatus from california for zika virus.zika virus (zikv) has emerged since 2013 as a significant global human health threat following outbreaks in the pacific islands and rapid spread throughout south and central america. severe congenital and neurological sequelae have been linked to zikv infections. assessing the ability of common mosquito species to transmit zikv and characterizing variation in mosquito transmission of different zikv strains is important for estimating regional outbreak potential and for prioritizing local mosquit ...201829927940
hydrophobic nanoprecipitates of β-cyclodextrin/avermectins inclusion compounds reveal insecticide activity against aedes aegypti larvae and low toxicity against fibroblasts.in the present work, hydrophobic nanoprecipitates (hnps) of inclusion complexes formed between β-cyclodextrin (βcd) and the avermectins (avms) named eprinomectin (epri) and ivermectin (iver) were synthesized and characterized, and their larvicidal activity against aedes aegypti and human safety against fibroblasts were evaluated. initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of avms in the presence of βcd. nuclear ...201829925239
field testing & evaluation of the efficacy & duration of effectiveness of a biolarvicide, bactivec® sc (bacillus thuringiensis var. israelensis sh-14) in bengaluru, india.different formulations of bacillus thuringiensis var. israelensis (bti) have been tested against different mosquito vectors and other insects for their residual activity. in the present study, the efficacy and residual activity of a new formulation of bti (bactivec suspension concentrate) were evaluated against immature stages of anopheles stephensi liston (diptera: culicidae), aedes aegypti linnaeus (diptera: culicidae) and culex quinquefasciatus say (diptera: culicidae), in natural habitats in ...201829923520
human blood as the only source of aedes aegypti in churches from merida, yucatan, mexico. 201929916450
salinity tolerant aedes aegypti and ae. albopictus-infection with dengue virus and contribution to dengue transmission in a coastal peninsula.aedes aegypti and ae. albopictus are major arboviral vectors that are considered to lay eggs, and undergo preimaginal development only in fresh water collections. however, recently they have been also shown to develop in coastal brackish water habitats. the ability of the biologically variant salinity-tolerant aedes vectors to transmit arboviral diseases is unknown. we therefore, investigated the infection of salinity-tolerant aedes mosquitoes with dengue virus (denv) and analysed dengue inciden ...201829916445
screening of natural wolbachia infection in aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus from guadeloupe (french west indies).guadeloupe islands are threatened by several mosquito-borne viruses such as dengue, chikungunya, zika and west nile virus. it appears essential to look for alternative mosquito control methods such as the incompatible insect technique (itt) aiming at sterilizing wild females by inundative releases of incompatible males. before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium wolbachia regarding the local mosquito populatio ...201829908171
prediction and prevention of urban arbovirus epidemics: a challenge for the global virology community.the recent emergence and rapid spread of zika virus in tropical regions of the western hemisphere took arbovirologists and public health officials by surprise, and the earlier transfers of west nile and chikungunya viruses from the old to the new world were also unexpected. these pandemics underscore the increasing threat of zoonotic arboviruses, especially those that are capable of entering into human-amplified, urban transmission cycles transmitted by aedes (stegomyia) aegypti and sometimes ot ...201829906475
lack of evidence for natural wolbachia infections in aedes aegypti (diptera: culicidae).wolbachia is a genus of endosymbiotic bacteria that infects 66% of all insect species. its major impact on insects is in reproduction: sterility, production of one sex, and/or parthenogenesis. another effect was discovered when the disease-transmitting mosquito, aedes aegypti linnaeus (diptera: culicidae), was infected with wolbachia isolated from drosophila: infected female mosquitoes became less capable of transmitting diseases such as dengue fever and chikungunya. this has led to releases of ...201829901734
restoration of pyrethroid susceptibility in a highly resistant aedes aegypti population.insecticide resistance has evolved in disease vectors worldwide, creating the urgent need to either develop new control methods or restore insecticide susceptibility to regain use of existing tools. here we show that phenotypic susceptibility can be restored in a highly resistant field-derived strain of aedes aegypti in only 10 generations through rearing them in the absence of insecticide.201829899128
signifying zika: heterogeneity in the representations of the virus by history of infection.despite having been broadly advertised by the mass media, many negative consequences of the zika virus have been less significant than originally predicted. it is likely that after a few months from the epidemic's onset, personal experience with the virus has altered the person's way to deal with the disease. this study explores the relation between exposure to zika virus and the social representation of the epidemic. more specifically, one analyzes if increased exposure to the risk of zika infe ...201829898004
evaluation of the effects of the larvicides temephos on reproductive performance, embryofetal development and dna integrity of swiss mice.temephos is considered the gold standard by the ministry of health for controlling the larvae of the mosquito aedes aegypti. the present study evaluated the effects of temephos larvicide on the reproductive performance, embryo-fetal development and dna integrity of swiss mice. this study used 30 pregnant female mice: 10 were controls treated with drinking water at a dosage of 0.1 ml/10 g (body weight - b.w., administered orally - a.o.), and 20 were treated with temephos at doses of 0.0043 mg/kg ...201829891373
what does the future hold for yellow fever virus? (i).the recent resurgence of yellow fever virus (yfv) activity in the tropical regions of africa and south america has sparked renewed interest in this infamous arboviral disease. yellow fever virus had been a human plague for centuries prior to the identification of its urban transmission vector, the aedes (stegomyia) aegypti (linnaeus) mosquito species, and the development of an efficient live-attenuated vaccine, the yf-17d strain. the combination of vector-control measures and vaccination campaig ...201829890711
a transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, aedes aegypti.populations of aedes aegypti naturally exhibit variable susceptibility to dengue viruses. this natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. in particular, larval crowding was previously shown to reduce the susceptibility of adult females of a trinidad field isolate of a. aegypti to the dengue serotype 2 (jam1409) virus. here, we present the first whole transc ...201829889847
target and non-target toxicity of fern extracts against mosquito vectors and beneficial aquatic organisms.dengue and malaria are significant mosquito-borne diseases that are rapidly spread worldwide, mainly in temperate countries. pteridophytes were identified to be a significant source of novel mosquitocidal agents. the present research was to explore the eco-friendly larvicides from methanol extracts of ferns, viz., actiniopteris radiata, adiantum caudatum, cheilanthes swartzii, hemionitis arifolia and lycopodium clavatum. the larvicidal potential of the extracts screened using larvae of dengue ve ...201829885618
viral determinants and vector competence of zika virus transmission.zika virus (zikv) has emerged as a new global health threat. since its first discovery in zika forest in uganda, this virus has been isolated from several mosquito species, including aedes aegypti and aedes albopictus. the geographical distribution of these mosquito species across tropical and subtropical regions has led to several outbreaks, including the recent pandemic in brazil, followed by the pacific islands and other areas of north and south america. this has gained attention of the scien ...201829875751
multiscale approach to deciphering the molecular mechanisms involved in the direct and intergenerational effect of ibuprofen on mosquito aedes aegypti.the anti-inflammatory ibuprofen is a ubiquitous surface water contaminant. however, the chronic impact of this pharmaceutical on aquatic invertebrate populations remains poorly understood. in model insect aedes aegypti, we investigated the intergenerational consequences of parental chronic exposure to an environmentally relevant concentration of ibuprofen. while exposed individuals did not show any phenotypic changes, their progeny showed accelerated development and an increased tolerance to sta ...201829874051
effects of temephos, permethrin, and eucalyptus nitens essential oil on survival and swimming behavior of aedes aegypti and anopheles pseudopunctipennis (diptera: culicidae) larvae.an essential strategy to deal with mosquito-borne diseases is the control of larvae in their development sites. the mosquitoes anopheles pseudopunctipennis (theobald) (diptera: culicidae), a malaria vector, and aedes aegypti (l.) (diptera: culicidae), vector of dengue, zika, yellow fever, and chikungunya viruses, breed in very different habitats. insecticide treatments of mosquito larvae focus mainly on their lethal effects. however, insecticide degradation or the poor dosage of larvicides will ...201829873741
vertical transmission of dengue virus in the yogyakarta airport area.international health regulations controls international travel including human movement, disease vector, and imported items to prevent the spread of dengue, especially in seaports, airports, and border crossing posts. this study aimed to determine dengue transovarial transmission index (tti) and distribution of dengue virus in the areas around adisucipto airport of yogyakarta, indonesia.201829871615
differential effects of temperature and mosquito genetics determine transmissibility of arboviruses by aedes aegypti in argentina.aedes aegypti (l.) (diptera: culicidae) have a global distribution and are the primary vector of a number of mosquito-borne viruses responsible for epidemics throughout the americas. as in much of south america, the threat from pathogens including dengue virus (denv; flaviviridae, flavivirus) and chikungunya virus (chikv; togaviridae, alphavirus) has increased in argentina in recent years. the complexity of transmission cycles makes predicting the occurrence and intensity of arbovirus outbreaks ...201829869610
serosurvey of human antibodies recognizing aedes aegypti d7 salivary proteins in colombia.dengue is one of the most geographically significant mosquito-borne viral diseases transmitted by aedes mosquitoes. during blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. these can be related to the intensity of exposure to bites. some mosquito salivary proteins, such as d7 proteins, are known as potent allergens. the antibody response to d7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical informat ...201829868532
effect of the topical repellent para-menthane-3,8-diol on blood feeding behavior and fecundity of the dengue virus vector aedes aegypti.dengue fever is an acute disease caused by the dengue virus and transmitted primarily by the mosquito aedes aegypti. the current strategy for dengue prevention is vector control including the use of topical repellents to reduce mosquito biting. although n,n-diethyl-m-methylbenzamide (deet) is the most common active ingredient in topical repellent products, para-menthane-3,8-diol (pmd) is also used commercially. studies have indicated pmd reduced biting by 90⁻95% for up to 6⁻8 h, similar to the e ...201829867036
pyrethroid resistance in the dengue vector aedes aegypti in southeast asia: present situation and prospects for management.human arboviral diseases transmitted by aedes aegypti such as dengue, zika, chikungunya and yellow fever remain global public health threats to date. of these diseases, dengue fever is particularly prevalent in southeast asia. relentless vector control efforts are performed to curtail disease transmissions through which pyrethroid insecticides are broadly used as the first line of defense to control ae. aegypti, especially in the course of disease outbreaks. here, we compile the largest contempo ...201829866193
molecular and functional characterization of single-box high-mobility group b (hmgb) chromosomal protein from aedes aegypti.high-mobility group b (hmgb) proteins have highly conserved, unique dna-binding domains, hmg boxes, that can bind non-b-type dna structures, such as bent, kinked and unwound structures, with high affinity. hmgb proteins also promote dna bending, looping and unwinding. in this study, we determined the role of the aedes aegypti single hmg-box domain protein aahmgb; characterized its structure, spatiotemporal expression levels, subcellular localization, and nucleic acid binding activities; and comp ...201829859286
technologies to combat aedes mosquitoes: a model based on smart city.aedes aegypti and aedes albopictus mosquitoes are responsible for the transmission of diseases such as dengue fever, yellow fever, chikungunya fever, zika virus fever, some of which can cause irreversible central nervous system problems and death. this study investigates what technologies are being used for combatting and monitoring the aedes mosquitoes and to propose joining these technologies into a single and complete solution using the smart cities concept. a search for newscasts on google a ...201829857404
exposure of a diurnal mosquito vector to floral mimics: foraging responses, feeding patterns, and significance for sugar bait technology.food location by mosquitoes is mediated by resource-derived olfactory and visual signals. smell sensation is intermittent and dependent on the environment, whereas visual signals are continual and precede olfactory cues. success of mosquito bait technology, where olfactory cues are used for attraction, is being impeded by reduced attractiveness. despite proof that mosquitoes respond to colored objects, including those mimicking floral shape, and that they can discriminate among flowers, the impa ...201829856985
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