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genetic stability of live-attenuated zika vaccine candidates.zika virus (zikv) has drawn global attention as the etiologic agent of zika congenital syndrome in babies born to infected pregnant women. to prevent future zikv outbreaks and protect persons at risk for severe disease, we developed two live-attenuated vaccine (lav) candidates containing 10- or 20-nucleotide deletions in the 3'utr of the viral genome (δ10 and δ20). after a single-dose immunization, both δ10 and δ20 lavs protected mice and non-human primates against zikv infection. here, we chara ...201931493417
subgenomic flavivirus rna binds the mosquito dead/h-box helicase me31b and determines zika virus transmission by aedes aegypti.zika virus (zikv) is an arthropod-borne flavivirus predominantly transmitted by aedes aegypti mosquitoes and poses a global human health threat. all flaviviruses, including those that exclusively replicate in mosquitoes, produce a highly abundant, noncoding subgenomic flavivirus rna (sfrna) in infected cells, which implies an important function of sfrna during mosquito infection. currently, the role of sfrna in flavivirus transmission by mosquitoes is not well understood. here, we demonstrate th ...201931488709
applying fuzzy logic to assess the biogeographical risk of dengue in south america.over the last decade, reports about dengue cases have increase worldwide, which is particularly worrisome in south america due to the historic record of dengue outbreaks from the seventeenth century until the first half of the twentieth century. dengue is a viral disease that involves insect vectors, namely aedes aegypti and ae. albopictus, which implies that, to prevent and combat outbreaks, it is necessary to understand the set of ecological and biogeographical factors affecting both the vecto ...201931488198
waterproof, low-cost, long-battery-life sound trap for surveillance of male aedes aegypti for rear-and-release mosquito control programmes.sterile male rear-and-release programmes are of growing interest for controlling aedes aegypti, including use an "incompatible insect technique" (iit) to suppress transmission of dengue, zika, and other viruses. under iit, males infected with wolbachia are released into the suppression area to induce cytoplasmic incompatibility in uninfected populations. these and similar mosquito-release programmes require cost-effective field surveys of both sexes to optimize the locations, timing, and quantit ...201931488182
a graph-based approach for finding the dengue infection pathways in humans using protein-protein interactions.dengue virus (denv) is one of the deadly arboviruses, which is primarily transmitted by aedes aegypti, and causes dengue infection to the humans. according to who, every year around 390 million humans are affected by denv, of which around 50 million deaths are reported. knowledge of the various diseases caused by the denv would greatly encourage to understand the infection mechanism and help to design new antiviral drug discovery. we propose a quasi-clique and quasi-biclique algorithm to classif ...201931486690
vertical transmission of dengue virus in aedes aegypti and its role in the epidemiological persistence of dengue in central and southern mexico.aedes aegypti and aedes albopictus are the main mosquito species responsible for dengue virus (denv) transmission to humans in the tropical and subtropical regions of the world. the role of vertical transmission in the epidemiology of dengue and the maintenance of this arbovirus in nature during interepidemic periods remain poorly understood, and denv vertical transmission could sustain the existence of virus reservoirs within aedes populations.201931483936
engineered action at a distance: blood-meal-inducible paralysis in aedes aegypti.population suppression through mass-release of aedes aegypti males carrying dominant-lethal transgenes has been demonstrated in the field. where population dynamics show negative density-dependence, suppression can be enhanced if lethality occurs after the density-dependent (i.e. larval) stage. existing molecular tools have limited current examples of such genetic pest management (gpm) systems to achieving this through engineering 'cell-autonomous effectors' i.e. where the expressed deleterious ...201931479450
travelling arboviruses: a historical perspective.chikungunya, dengue, yellow fever and zika viruses share many attributes. all are complex and widespread zoonoses of subhuman primates that have made successful transitions to the urban aedes aegypti transmission cycle. more important, they have an established record of travelling, having moved from their place of origin hundreds of years ago, sometimes repeatedly. understanding their epidemiology requires a knowledge of past behaviors including unexplained restraints to their travel. this is a ...201931472285
identification of human skin bacteria attractive to the asian tiger mosquito.aedes albopictus is a vector of arboviruses and filarial nematodes. originating from asia, this mosquito has rapidly expanded its geographical distribution and colonized areas across both temperate and tropical regions. due to the increase in insecticide resistance, the use of environmentally friendly vector control methods is encouraged worldwide. using methods based on semiochemicals in baited traps are promising for management of mosquito populations. interestingly, human skin microbiota was ...201931464044
quantifying the efficacy of genetic shifting in control of mosquito-borne diseases.many of the world's most prevalent diseases are transmitted by animal vectors such as dengue transmitted by mosquitoes. to reduce these vector-borne diseases, a promising approach is "genetic shifting": selective breeding of the vectors to be more resistant to pathogens and releasing them to the target populations to reduce their ability to transmit pathogens, that is, lower their vector competence. the efficacy of genetic shifting will depend on possible counterforces such as natural selection ...201931462914
stable distinct core eukaryotic viromes in different mosquito species from guadeloupe, using single mosquito viral metagenomics.mosquitoes are the most important invertebrate viral vectors in humans and harbor a high diversity of understudied viruses, which has been shown in many mosquito virome studies in recent years. these studies generally performed metagenomics sequencing on pools of mosquitoes, without assessment of the viral diversity in individual mosquitoes. to address this issue, we applied our optimized viral metagenomics protocol (netovir) to compare the virome of single and pooled aedes aegypti and culex qui ...201931462331
climate drives spatial variation in zika epidemics in latin america.between 2015 and 2017, zika virus spread rapidly through populations in the americas with no prior exposure to the disease. although climate is a known determinant of many aedes-transmitted diseases, it is currently unclear whether climate was a major driver of the zika epidemic and how climate might have differentially impacted outbreak intensity across locations within latin america. here, we estimated force of infection for zika over time and across provinces in latin america using a time-var ...201931455188
breteau index as a promising early warning signal for dengue fever outbreaks in the colombo district, sri lanka.despite the efforts in reducing vector densities, outbreaks of dengue fever have become a frequent event in sri lanka. as explained by dengue transmission dynamics, vector control activities intensified at peak or near peak outbreak situations would not be successful in controlling the outbreaks. a reliable method of outbreak prediction is always warranted for early preparedness. relationships between the monthly breteau indices of the two vector species (aedes aegypti l. and ae. albopictus skus ...201931454507
investigating the impact of chilling temperature on male aedes aegypti and aedes albopictus survival.in genetic control programmes, including the sterile insect technique (sit), it is crucial to release insects of the highest quality with maximum survival. it is likely that male mosquitoes will follow the trend of other insects in sit programmes and be stored, transported and eventually released under chilled conditions. the aim of our study was to investigate the impact of different chilling temperatures on male aedes aegypti and ae. albopictus survival by exposing them to a range of temperatu ...201931454400
selection on aedes aegypti alters wolbachia-mediated dengue virus blocking and fitness.the dengue, zika and chikungunya viruses are transmitted by the mosquito aedes aegypti and pose a substantial threat to global public health. current vaccines and mosquito control strategies have limited efficacy, so novel interventions are needed1,2. wolbachia are bacteria that inhabit insect cells and have been found to reduce viral infection-a phenotype that is referred to as viral 'blocking'3. although not naturally found in a. aegypti4, wolbachia were stably introduced into this mosquito in ...201931451771
mosquito bite prevention through graphene barrier layers.graphene-based materials are being developed for a variety of wearable technologies to provide advanced functions that include sensing; temperature regulation; chemical, mechanical, or radiative protection; or energy storage. we hypothesized that graphene films may also offer an additional unanticipated function: mosquito bite protection for light, fiber-based fabrics. here, we investigate the fundamental interactions between graphene-based films and the globally important mosquito species, aede ...201931451645
host and viral mechanisms of congenital zika syndrome.in 2015-2016, in the americas, and especially in northeast brazil, a significant number of cases of microcephaly and other congenital brain abnormalities were linked with an outbreak of zika virus (zikv) infection in pregnant women. while maternal symptoms of zikv are generally mild and self-limiting, clinical presentation in fetuses and newborns infected is extensive and includes microcephaly, decreased cortical development, atrophy and hypoplasia of the cerebellum and cerebellar vermis, arthro ...201931451049
life history traits and predatory performance of belostoma anurum (hemiptera: belostomatidae), a biological control agent of disease vector mosquitoes.understanding the life cycle and dietary requirements of laboratory-reared insects is critical for optimizing resources (including time) and can provide more reliable ecological basis for using such biological control agents in realistic programs. here, we evaluated the complete development and the predatory abilities of belostoma anurum (herrich-schäffer, 1848) (hemiptera: belostomatidae), an aquatic predator widely distributed in neotropical region, when reared at different diets. we firstly i ...201931446550
exposure of mosquito (aedes aegypti) larvae to the water extract and lectin-rich fraction of moringa oleifera seeds impairs their development and future fecundity.aedes aegypti control is a key component of the prophylaxis of dengue fever and other diseases. moringa oleifera seeds contain a water-soluble lectin (wsmol) with larvicidal and ovicidal activities against this insect. in this study, a. aegypti individuals were exposed at the third larval instar for 24 h to the water extract (0.1-1.0 mg/ml of protein) or lectin-rich fraction (0.05-0.6 mg/ml of protein) containing wsmol, and then their survival and development were followed for 9 days post-exposu ...201931446169
mitochondrial glycerol phosphate oxidation is modulated by adenylates through allosteric regulation of cytochrome c oxidase activity in mosquito flight muscle.the huge energy demand posed by insect flight activity is met by an efficient oxidative phosphorylation process that takes place within flight muscle mitochondria. in the major arbovirus vector aedes aegypti, mitochondrial oxidation of pyruvate, proline and glycerol 3-phosphate (g3p) represent the major energy sources of atp to sustain flight muscle energy demand. although adenylates exert critical regulatory effects on several mitochondrial enzyme activities, the potential consequences of alter ...201931446033
deet and other repellents are inhibitors of mosquito odorant receptors for oviposition attractants.in addition to its primary function as an insect repellent, deet has many "off-label" properties, including a deterrent effect on the attraction of gravid female mosquitoes. deet negatively affects oviposition sites. while deorphanizing odorant receptors (ors) using the xenopus oocyte recording system, we have previously observed that deet generated outward (inhibitory) currents on ors sensitive to oviposition attractants. here, we systematically investigated these inhibitory currents. we record ...201931446031
increased akt signaling in the fat body of anopheles stephensi extends lifespan and increases lifetime fecundity through modulation of insulin-like peptides.insulin-like peptides (ilps) and the insulin/insulin-like growth factor 1 signaling (iis) cascade regulate numerous physiological functions, including lifespan, reproduction, immunity, and metabolism, in diverse eukaryotes. we previously demonstrated that in female anopheles stephensi and aedes aegypti mosquitoes, activation of the iis cascade in the fat body led to a significant increase in lifespan. in this work, we elucidated two putative mechanisms in a. stephensi behind the observed lifespa ...201931445957
heterologous production and functional characterization of ageritin, a novel type of ribotoxin highly expressed during fruiting of the edible mushroom agrocybe aegerita.fungi produce various defense proteins against antagonists, including ribotoxins. these toxins cleave a single phosphodiester bond within the universally conserved sarcin-ricin loop of ribosomes and inhibit protein biosynthesis. here, we report on the structure and function of ageritin, a previously reported ribotoxin from the edible mushroom agrocybe aegerita the amino acid sequence of ageritin was derived from cdna isolated from the dikaryon a. aegerita aae-3 and lacks, according to in silico ...201931444206
investigation of relative development and reproductivity fitness cost in three insecticide-resistant strains of aedes aegypti from thailand.knockdown resistance (kdr) and detoxification enzymes are major resistance mechanisms in insecticide-resistant aedes aegypti throughout the world. persistence of the resistance phenotype is associated with high fitness of resistance alleles in the absence of insecticide pressure. this study determined the relative fitness cost of three insecticide-resistant strains of aedes aegypti-pmd, pmd-r, and upk-r-and a hybrid under similar laboratory conditions in the absence of insecticide. the pmd strai ...201931443487
odorant receptors from culex quinquefasciatus and aedes aegypti sensitive to floral compounds.mosquitoes rely heavily on the olfactory system to find a host for a bloodmeal, plants for a source of energy and suitable sites for oviposition. here, we examined a cluster of eight odorant receptors (ors), which includes one or, cquior1, previously identified to be sensitive to plant-derived compounds. we cloned 5 ors from culex quinquefasciatus and two ors from aedes aegypti, ie, cquior2, cquior4, cquior5, cquior84, cquior85, aaegor14, and aaegor15 and then deorphanized these receptors using ...201931442487
first report on the transmission of zika virus by aedes (stegomyia) aegypti (l.) (diptera: culicidae) during the 2018 zika outbreak in india.in a recent outbreak of zika virus (zikv) infection in jaipur city (rajasthan, india), a total of 159 cases were reported in september 2018. in order to identify vector responsible for zika transmission, mosquitoes were collected from houses with reported zika cases and nearby houses. a total of 108 pools containing 522 mosquitoes were tested for presence of zikv using rt-pcr and real time rt-pcr. we detected presence of zikv in three pools of aedes (stegomyia) aegypti (l.), out of a total of 79 ...201931442386
new county records of aedes aegypti and aedes epactius in colorado.in august and september 2017, we conducted mosquito surveillance in southeastern colorado by using ovitraps and larval sampling. the aim was to determine if there were established populations of aedes aegypti and ae. albopictus in the region. a single female ae. aegypti was reared from eggs collected in la junta, co, but ae. albopictus was not detected. three other species were reared from eggs and/or larvae: ae. epactius, culex restuans, and cx. pipiens. aedes aegypti and ae. epactius were dete ...201931442179
endemic disease control agents' perception on the fight against aedes aegypti and the prevention of arbovirus infections in brazil.arboviruses pose a serious and constant threat to public health, and have demanded surveillance efforts worldwide. the prevention of arbovirus transmission depends on effective measures to control vectors and promote health. the objective of this study was to examine the factors that enhance and impair the endemic disease control agents' field work, based on their own perspective.201931584945
vectors of arboviruses in the state of são paulo: 30 years of aedes aegypti and aedes albopictus.to describe the infestation of the municipalities of são paulo by the vectors aedes aegypti and aedes albopictus, characterize seasonality and analyze average temperatures and larval densities.201931576944
knockout of juvenile hormone receptor, methoprene-tolerant, induces black larval phenotype in the yellow fever mosquito, aedes aegypti.the yellow fever mosquito, aedes aegypti, vectors human pathogens. juvenile hormones (jh) control almost every aspect of an insect's life, and jh analogs are currently used to control mosquito larvae. since rna interference does not work efficiently during the larval stages of this insect, jh regulation of larval development and mode of action of jh analogs are not well studied. to overcome this limitation, we used a multiple single guide rna-based crispr/cas9 genome-editing method to knockout t ...201931570611
discovery of an aedes (stegomyia) albopictus population and first records of aedes (stegomyia) aegypti in canada.a population of aedes (stegomyia) albopictus (skuse) (diptera: culicidae), a vector of chikungunya, dengue, yellow fever, and zika and west nile viruses, has been detected in windsor, ontario, canada from 2016 onwards. here, we describe its seasonal distribution, as well as the various aquatic habitats from which this species was collected and its larval co-habitation. we collected immatures from tires, treeholes, extruded polystyrene foam containers, discarded plastic cups, old recycling bins a ...201931566765
cluster containment strategy: addressing zika virus outbreak in rajasthan, india.india is at risk of zika virus transmission due to high prevalence of its vector aedes aegypti. rajasthan, a state in the north-west region of india, has also high prevalence of aedes mosquito. first laboratory confirmed case of zika virus disease in rajasthan was reported on 21 september 2018 in jaipur. the government of rajasthan quickly implemented a containment strategy to contain the outbreak and prevent further spread of this disease. strategy included active human and mosquito surveillanc ...201931565402
action of metarhizium brunneum (hypocreales: clavicipitaceae) against organophosphate- and pyrethroid-resistant aedes aegypti (diptera: culicidae) and the synergistic effects of phenylthiourea.dengue, yellow fever, zika, and chikungunya arboviruses are endemic in tropical countries and are transmitted by aedes aegypti. resistant populations of this mosquito against chemical insecticides are spreading worldwide. this study aimed to evaluate the biological effects of exposure of pesticide-sensitive ae. aegypti larvae (rockefeller) to conidia of the entomopathogen, metarhizium brunneum, laboratory strains arsef 4556 and v275, and any synergistic activity of phenylthiourea (ptu). in addit ...201931559435
evidence-based process for prioritizing positive behaviors for promotion: zika prevention in latin america and the caribbean and applicability to future health emergency responses.since the 2015 zika outbreak in latin america and the caribbean, a plethora of behavior change messages have been promoted to reduce zika transmission. one year after the united states agency for international development (usaid) initiated its zika response, more than 30 variants of preventive behaviors were being promoted. this situation challenged social and behavior change (sbc) programming efforts that require a coordinated response and agreed upon set of focus behaviors to be effective. to ...201931558597
de novo genome sequencing and comparative stage-specific transcriptomic analysis of dirofilaria repens.the zoonotic mosquito-borne filarial nematode dirofilaria repens causes subcutaneous and ocular infections in dogs, cats and humans. from infected vertebrate hosts, microfilariae are taken up by mosquitoes and develop into infective l3. these are transmitted to new vertebrate hosts and develop over two further moults to adult worms. the aims of the project were (i) the de novo sequencing and annotation of the d. repens genome and (ii) comparative transcriptomic analyses of the two developmental ...201931557466
globe-trotting aedes aegypti and aedes albopictus: risk factors for arbovirus pandemics.introduction: two species of aedes (ae.) mosquitoes (ae. aegypti and ae. albopictus) are primary vectors for emerging arboviruses that are a significant threat to public health and economic burden worldwide. distribution of these vectors and the associated arboviruses, such as dengue virus, chikungunya virus, yellow fever virus, and zika virus, was for a long time restricted by geographical, ecological, and biological factors. presently, arbovirus emergence and dispersion are more rapid and geog ...201931556813
reducing the cost and assessing the performance of a novel adult mass-rearing cage for the dengue, chikungunya, yellow fever and zika vector, aedes aegypti (linnaeus).the widespread emergence of resistance to insecticides used to control adult aedes mosquitoes has made traditional control strategies inadequate for the reduction of various vector populations. therefore, complementary vector control methods, such as the sterile insect technique, are needed to enhance existing efforts. the technique relies on the rearing and release of large numbers of sterile males, and the development of efficient and standardized mass-rearing procedures and tools is essential ...201931553724
liaisons dangereuses: cross-border gene flow and dispersal of insecticide resistance-associated genes in the mosquito aedes aegypti from brazil and french guiana.in recent years, south america has suffered the burden of continuous high impact outbreaks of dengue, chikungunya and zika. aedes aegypti is the main mosquito vector of these arboviruses and its control is the only solution to reduce transmission.201931553370
aedes spp. and their microbiota: a review.aedes spp. are a major public health concern due to their ability to be efficient vectors of dengue, chikungunya, zika, and other arboviruses. with limited vaccines available and no effective therapeutic treatments against arboviruses, the control of aedes spp. populations is currently the only strategy to prevent disease transmission. host-associated microbes (i.e., microbiota) recently emerged as a promising field to be explored for novel environmentally friendly vector control strategies. in ...201931551973
non-canonical transcriptional regulation of heme oxygenase in aedes aegypti.heme oxygenase (ho) is a ubiquitous enzyme responsible for heme breakdown, which yields carbon monoxide (co), biliverdin (bv) and ferrous ion. here we show that the aedes aegypti heme oxygenase gene (aeho - aael008136) is expressed in different developmental stages and tissues. aeho expression increases after a blood meal in the midgut, and its maximal transcription levels overlaps with the maximal rate of the further modified a. aegypti biglutaminyl-biliverdin (aebv) pigment production. ho is a ...201931551499
conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy.one of the strategies of integrated vector management is to lure gravid mosquitoes for surveillance purposes or to entice them to lay eggs in water containing toxins that kill the offspring (attract-and-kill or trap-and-kill). typically, the major challenge of this approach is the development of a lure that stimulates oviposition plus a toxin with no deterrent effect. bacillus thuringiensis var. israelensis (bti) satisfies the latter criterion, but lures for these autocidal gravid traps are sore ...201931551447
serine hydroxymethyltransferase controls blood-meal digestion in the midgut of aedes aegypti mosquitoes.female aedes aegypti mosquitoes are vectors of arboviruses that cause diverse diseases of public health significance. blood protein digestion by midgut proteases provides anautogenous mosquitoes with the nutrients essential for oocyte maturation and egg production. midgut-specific mir-1174 affects the functions of the midgut through its target gene serine hydroxymethyltransferase (shmt). however, less is known about shmt-regulated processes in blood digestion by mosquitoes.201931551071
temperature and sugar feeding effects on the activity of a laboratory strain of aedes aegypti.aedes aegypti is an invasive mosquito species that is expected to expand its global distribution through climate change. as poikilotherms, mosquitoes are greatly affected by the temperature of the environment which can impact host-seeking, blood-feeding, and flight activity as well as survival and ability to transmit pathogens. however, an important aspect of mosquito biology on which the effect of temperature has not been investigated is water and sugar-feeding and how access to a sugar source ...201931623118
control of rna viruses in mosquito cells through the acquisition of vdna and endogenous viral elements.aedes aegypti transmit pathogenic arboviruses while the mosquito itself tolerates the infection. we examine a pirna-based immunity that relies on the acquisition of viral derived cdna (vdna) and how this pathway discriminates between self and non-self. the pirnas derived from these vdnas are essential for virus control and piwi4 has a central role in the pathway. piwi4 binds preferentially to virus-derived pirnas but not to transposon-targeting pirnas. analysis of episomal vdna from infected cel ...201931621580
analysis in a murine model points to igg responses against the 34k2 salivary proteins from aedes albopictus and aedes aegypti as novel promising candidate markers of host exposure to aedes mosquitoes.aedes mosquitoes are vectors of arboviral diseases of great relevance for public health. the recent outbreaks of dengue, zika, chikungunya and the rapid worldwide spreading of aedes albopictus emphasize the need for improvement of vector surveillance and control. host antibody response to mosquito salivary antigens is emerging as a relevant additional tool to directly assess vector-host contact, monitor efficacy of control interventions and evaluate risk of arboviral transmission.201931618201
amylose inclusion complexes as emulsifiers for garlic and asafoetida essential oils for mosquito control.although the insecticidal properties of some plant essential oils are well-documented, their use in integrated pest and vector management is complicated by their high volatility, low thermal stability, high sensitivity to oxidation, and low solubility in water. we investigated the use of bio-based n-1-hexadecylammonium chloride and sodium palmitate amylose inclusion complexes as emulsifiers for two essential oils, garlic and asafoetida, known to be highly toxic to mosquito larvae. four emulsions ...201931614606
efficacy of active ingredients from the epa 25(b) list in reducing attraction of aedes aegypti (diptera: culicidae) to humans.mosquitoes of the aedes genus are vectors for dengue, chikungunya, zika, and yellow fever viruses. mosquito repellents are an effective way to prevent mosquito bites and reduce the spread of mosquito-borne diseases. in the early 90s, the u.s. environmental protection agency (epa) published a list of active ingredients that pose minimum risk to human health that can be used as pesticides or repellents without passing the epa registration process. the present study examined the efficacy of 21 of t ...201931612914
iron and ferritin deposition in the ovarian tissues of the yellow fever mosquito (diptera: culicidae).dengue, yellow fever, and zika are viruses transmitted by yellow fever mosquito, aedes aegypti [linnaeus (diptera: culicidae)], to thousands of people each year. mosquitoes transmit these viruses while consuming a blood meal that is required for oogenesis. iron, an essential nutrient from the blood meal, is required for egg development. mosquitoes receive a high iron load in the meal; although iron can be toxic, these animals have developed mechanisms for dealing with this load. our previous res ...201931606748
efficacy of long-lasting insecticidal nets with declining physical and chemical integrity on aedes aegypti (diptera: culicidae).fitting long-lasting insecticidal nets (llin) as screens on doors/windows has a significant impact on indoor-adult aedes aegypti (l.), with entomological reductions measured in a previous study being significant for up to 2 yr post-installation, even in the presence of pyrethroid-resistant aedes populations. to better understand the mode of llin protection, bioassays were performed to evaluate the effects of field deployment (0, 6, and 12 mo) and damage type (none, central, lateral, and multiple ...201931603517
inhibition of dicer activity in lepidopteran and dipteran cells by baculovirus-mediated expression of flock house virus b2.prior studies have suggested that insect dna viruses are negatively affected by dicer-2-mediated rna interference (rnai). to examine this further, we utilized an in vitro assay to measure dicer activity in lepidopteran and dipteran cells, combined with baculoviruses expressing the rnai suppressor b2 from flock house virus or aedes aegypti dicer-2 (aedicer-2) using a constitutive heat shock promoter. addition of cell lysates containing baculovirus-expressed b2 to lysates from dipteran (s2, aag2) ...201931601846
differential susceptibility and innate immune response of aedes aegypti and aedes albopictus to the haitian strain of the mayaro virus.mayaro (mayv) is an emerging arthropod-borne virus belonging to the alphavirus genus of the togaviridae family. although forest-dwelling haemagogus mosquitoes have been considered as its main vector, the virus has also been detected in circulating aedes ssp mosquitoes. here we assess the susceptibility of aedes aegypti and aedes albopictus to infection with mayv and their innate immune response at an early stage of infection. aedes albopictus was more susceptible to infection with mayv than ae. ...201931601017
management of insecticide resistance in the major aedes vectors of arboviruses: advances and challenges.the landscape of mosquito-borne disease risk has changed dramatically in recent decades, due to the emergence and reemergence of urban transmission cycles driven by invasive aedes aegypti and ae. albopictus. insecticide resistance is already widespread in the yellow fever mosquito, ae. aegypti; is emerging in the asian tiger mosquito ae. albopictus; and is now threatening the global fight against human arboviral diseases such as dengue, yellow fever, chikungunya, and zika. because the panel of i ...201931600206
consensus and uncertainty in the geographic range of aedes aegypti and aedes albopictus in the contiguous united states: multi-model assessment and synthesis.aedes (stegomyia) aegypti (l.) and ae. (stegomyia) albopictus (skuse) mosquitoes can transmit dengue, chikungunya, yellow fever, and zika viruses. limited surveillance has led to uncertainty regarding the geographic ranges of these vectors globally, and particularly in regions at the present-day margins of habitat suitability such as the contiguous united states. empirical habitat suitability models based on environmental conditions can augment surveillance gaps to describe the estimated potenti ...201931600194
urbanization creates diverse aquatic habitats for immature mosquitoes in urban areas.global increases in temperatures and urbanization are impacting the epidemiology of mosquito-borne diseases. urbanization processes create suitable habitats for vector mosquitoes in which there are a reduced number of predators, and human hosts are widely available. we hypothesize that mosquito vector species, especially aedes aegypti, are locally concentrated primarily in those specific habitats at the neighborhood levels that provide suitable conditions and environmental resources needed for m ...201931653914
vector mosquito surveillance using centers for disease control and prevention autocidal gravid ovitraps in san antonio, texas.mosquito surveillance in large urban areas of the southern usa that border mexico has become increasingly important due to recent transmission of zika virus and chikungunya virus in the americas as well as the continued threat of dengue and west nile viruses. the vectors of these viruses, aedes aegypti, ae. albopictus, and culex quinquefasciatus, co-occur in residential areas, requiring vector control entities to deploy several different trap types, often expensive and labor-intensive, to survei ...201931647715
a milestone in botanical mosquito repellents: novel pmd-based formulation protects more than twice as long as high-concentration deet and other leading products.the use of skin-applied repellents is the primary method recommended by the centers for disease control and prevention for personal protection against biting mosquitoes. historically, the majority of long-efficacy mosquito repellents have been n,n diethyl-3-methylbenzamide (deet)-based. recently, a variety of new botanical formulations have been marketed, but their protection times generally continue to fall well short of high-concentration deet products. we present a laboratory arm-in-cage stud ...201931647713
from surveillance to control: evaluation of a larvicide intervention against aedes aegypti in brownsville, texas.south texas is recognized as a potential area for the emergence and re-emergence of mosquito-borne diseases due to recent circulation of zika, chikungunya, and dengue viruses. during 2017, high aedes aegypti abundance found in the city of brownsville, tx, in combination with the previous year's local transmission of zika virus, triggered the activation of the texas department of state health services emergency mosquito control contingency contract. a contract with the clarke environmental and mo ...201931647710
new record of aedes albopictus in a suburban area of merida, yucatan, mexico.a survey was carried out in 51 households within a suburban area of merida, yucatan, mexico, for 5 consecutive days. adult collections were performed using prokopack aspirators (indoors) and human-landing mosquito catches (hlc) outdoors, and larval sites (artificial containers) were revised for larvae collection. a total of 259 aedes albopictus were collected, 246 from artificial larval sites, 8 by indoor aspiration, and 5 by hlc. this is the first record of ae. albopictus in merida, yucatan, me ...201931647708
evaluation of the persistence of three larvicides used to control aedes aegypti in arapiraca, northeastern brazil.the chemical control of the mosquito aedes aegypti is a great challenge worldwide, since several populations of this species are already resistant to traditional insecticides, such as temephos. in brazil, alternative larvicides, such as bacillus thuringiensis israelensis (bti) and pyriproxyfen, have been used more recently. in this study we evaluated the persistence of pyriproxyfen (sumilarv 0.5%g), 2 commercial formulations of bti (vectobac wdg and vectobac g), and temephos (fersol 1g) under fi ...201931647707
a novel methodology for recording wing beat frequencies of untethered male and female aedes aegypti.aedes aegypti is a vector of many significant arboviruses worldwide, including dengue, zika, chikungunya, and yellow fever viruses. with vector control methodology pivoting toward rearing and releasing large numbers of insects for either population suppression or virus-blocking, economical remote (sentinel) surveillance methods for release tracking become increasingly necessary. recent steps in this direction include advances in optical sensors that identify and classify insects based on their w ...201931647706
[recurrence of dengue epidemics in burkina faso: community preference for an intervention to prevent the disease].community-based interventions have proven effective in several latin american countries in controlling dengue vector aedes aegypti and reducing the burden of the disease. however, we did not find any study reporting the assessment or implementation of such interventions in sub-saharan africa. this article presents local communities' preferences for activities as part of the implementation of a community-based intervention for dengue prevention in ouagadougou (burkina faso) where dengue epidemics ...201931645291
susceptibility to insecticides and resistance mechanisms in three populations of aedes aegypti from peru.epidemics of dengue, chikungunya and zika are a growing threat to areas where aedes aegypti are present. the efficacy of chemical control of ae. aegypti is threatened by the increasing frequency of insecticide resistance. the objective of this study was to determine the susceptibility status as well as the biochemical and molecular mechanisms underlying insecticide resistance in three populations of ae. aegypti in high risk areas of dengue, chikungunya, and zika in peru.201931640810
does membrane feeding compromise the quality of aedes aegypti mosquitoes?modified aedes aegypti mosquitoes are being mass-reared for release in disease control programs around the world. releases involving female mosquitoes rely on them being able to seek and feed on human hosts. to facilitate the mass-production of mosquitoes for releases, females are often provided blood through artificial membrane feeders. when reared across generations there is a risk that mosquitoes will adapt to feeding on membranes and lose their ability to feed on human hosts. to test adaptat ...201931693672
aedes aegypti (aag2)-derived clonal mosquito cell lines reveal the effects of pre-existing persistent infection with the insect-specific bunyavirus phasi charoen-like virus on arbovirus replication.aedes aegypti is a vector mosquito of major public health importance, transmitting arthropod-borne viruses (arboviruses) such as chikungunya, dengue, yellow fever and zika viruses. wild mosquito populations are persistently infected at high prevalence with insect-specific viruses that do not replicate in vertebrate hosts. in experimental settings, acute infections with insect-specific viruses have been shown to modulate arbovirus infection and transmission in ae. aegypti and other vector mosquit ...201931693659
urban landscape features influence the movement and distribution of the australian container-inhabiting mosquito vectors aedes aegypti (diptera: culicidae) and aedes notoscriptus (diptera: culicidae).urban landscape features play an important role in the distribution and population spread of mosquito vectors. furthermore, current insecticide and novel rear-and-release strategies for urban mosquito management rarely consider the spatial structure of the landscape when applying control practices. here, we undertake a mark-recapture experiment to examine how urban features influence the movement and distribution of australian container-inhabiting aedes vectors. we pay attention to the role of s ...201931693154
comparison of two dna extraction methods from larvae, pupae, and adults of aedes aegypti.mosquitoes are the most important arthropods from the point of view of public health, due to the fact that they can transmit a large number of pathogens which can cause diseases to humans and animals. aedes aegypti (l.) is one of the most important vector species in the world, since it can transmit numerous pathogens such as dengue, zika, and chikungunya. therefore, studies involving the molecular aspects of this and other mosquitoes species are currently increasing. in this report, we describe ...201931692696
soil presence reduces the control effectiveness of a slow-release formulation of pyriproxyfen on aedes aegypti (diptera: culicidae) larvae.to assess the influence of soil on the effectiveness of two new slow-release formulations (floating and non-floating) of pyriproxyfen coextruded with low-density polyethylene.201931691403
aedes vector-host olfactory interactions in sylvatic and domestic dengue transmission environments.interactions between aedes (stegomyia) species and non-human primate (nhp) and human hosts govern the transmission of the pathogens, dengue, zika, yellow fever and chikungunya viruses. little is known about aedes mosquito olfactory interactions with these hosts in the domestic and sylvatic cycles where these viruses circulate. here, we explore how the different host-derived skin odours influence aedes mosquito responses in these two environments. in field assays, we show that the cyclic ketone c ...201931690238
a popular indian clove-based mosquito repellent is less effective against culex quinquefasciatus and aedes aegypti than deet.insect repellents are widely used as the first line of defense against mosquito bites and transmission of disease-causing agents. however, the cost of daily applications of even the most affordable and the gold standard of insect repellents, deet, is still high for low-income populations where repellents are needed the most. an indian clove-based homemade recipe has been presented as a panacea. we analyzed this homemade repellent and confirmed by behavioral measurements and odorant receptor resp ...201931689339
an alternative model to explain the vectorial capacity using as example aedes aegypti case in dengue transmission.vectorial capacity (vc), as a concept that describes the potential of a vector to transmit a pathogen, has had historical problems related to lacks in dimensional significance and high error propagation from parameters that take part in the model to output. hence, values estimated with those equations are not sufficiently reliable to consider in control strategies or vector population study. in this paper, we propose a new vc model consistent at dimensional level, i.e., the definition and the eq ...201931687486
effect of larval nutritional regimes on morphometry and vectorial capacity of aedes aegypti for dengue transmission.nutritional level in larval diet of mosquito vectors influence on life history traits and vectorial capacity (vc). therefore, the present study was carried out to assess the effect of larval diet concentration on vector bionomic and vc of aedes aegypti in sri lanka.201931687387
insecticidal activities of citrus aurantifolia essential oil against aedes aegypti (diptera: culicidae).in the recent time, global attention for the control of vectors has shifted from chemical insecticides to botanicals. in the present investigation, authors attempted to evaluate the efficacy of peel and leaf essential oil (eo) of citrus aurantifolia against aedes aegypti.201931687359
bacteria-mediated modification of insecticide toxicity in the yellow fever mosquito, aedes aegypti.the incidence of mosquito-borne disease poses a significant threat to human and animal health throughout the world, with effective chemical control interventions limited by widespread insecticide resistance. recent evidence suggests that gut bacteria of mosquitoes, known to be essential in nutritional homeostasis and pathogen defense, may also play a significant role in facilitating insecticide resistance. this study investigated the extent to which bacteria contribute to the general esterase an ...201931685200
insecticidal and repellent properties of novel trifluoromethylphenyl amides iii.the adulticidal, larvicidal, and repellent activity of 18 trifluoromethylphenyl amides (tfmpas) was determined against aedes aegypti mosquitoes. the compounds studied are the third generation designed from active structures of the previous two generations. n-(3,5-bis(trifluoromethyl)phenyl)-2-chloroacetamide (8f) and n-(3,5-bis(trifluoromethyl)phenyl)-2,2,3,3,3-pentafluoropropanamide (8h) were most active against 1st stage ae. aegypti larvae with lc50 values of 125 and 2.53 μm; for comparative p ...201931685196
entomological surveillance to assess potential outbreak of aedes-borne arboviruses and insecticide resistance status of aedes aegypti from cape coast, ghana.this study was conducted in cape coast, a major tourist destination in ghana to assess the risk of an outbreak of aedes-borne arboviruses based on entomological indicators and determine their susceptibility to pyrethroid insecticides. a larval survey was conducted in 414 houses between april and july 2017. larvae collected were reared to adult for who susceptibility bioassay against four pyrethroid insecticides (deltamethrin 0.05%, permethrin 0.75%, cyfluthrin 0.15%, etofenprox 0.5%) and three d ...201931682813
three dimensional secondary ion mass spectrometry imaging (3d-sims) of aedes aegypti ovarian follicles.the mobilization of nutrient reserves into the ovaries of aedes aegypti mosquitoes after sugar-feeding plays a vital role in the female's reproductive maturation. in the present work, three-dimensional secondary ion mass spectrometry imaging (3d-sims) was used to generate ultrahigh spatial resolution (~1 μm) chemical maps and study the composition and spatial distribution of lipids at the single ovarian follicle level (~100 μm in size). 3d-mass spectrometry imaging (3d-msi) allowed the identific ...201931680712
mosquitocidal activity of the pale brittle stem mushroom, psathyrella candolleana (agaricomycetes), against three vector mosquitoes.the aim of the present study was to screen for mosquito larvicidal activity of methanol extract of a wild mushroom psathyrella candolleana against culex quinquefasciatus, aedes aegypti, and anopheles stephensi. larvae and eggs were exposed to four different concentrations (62.5, 125, 250, and 500 ppm) and mortality was assessed 24 and 120 h after treatment. the lc50 and lc90 values of the extract were calculated. methanol crude extract of p. candolleana showed good larvicidal activity against th ...201931679306
blockade of dengue virus transmission from viremic blood to aedes aegypti mosquitoes using human monoclonal antibodies.dengue is the most prevalent arboviral disease of humans. virus neutralizing antibodies are likely to be critical for clinical immunity after vaccination or natural infection. a number of human monoclonal antibodies (mabs) have previously been characterized as able to neutralize the infectivity of dengue virus (denv) for mammalian cells in cell-culture systems.201931675360
potential inhibitors of the enzyme acetylcholinesterase and juvenile hormone with insecticidal activity: study of the binding mode via docking and molecular dynamics simulations.models validation in qsar, pharmacophore, docking and others can ensure the accuracy and reliability of future predictions in design and selection of molecules with biological activity. in this study, pyriproxyfen was used as a pivot/template to search the database of the maybridge database for potential inhibitors of the enzymes acetylcholinesterase and juvenile hormone as well. the initial virtual screening based on the 3d shape resulted in 2000 molecules with tanimoto index ranging from 0.58 ...201931674282
green synthesis of silver nanoparticles using solanum mammosum l. (solanaceae) fruit extract and their larvicidal activity against aedes aegypti l. (diptera: culicidae).the family of mosquitoes (diptera: culicidae) contains several species of major public health relevance due to their role as vectors of human disease. one of these species, aedes aegypti, is responsible for the transmission of some of the most important vector-borne viruses affecting humankind, including dengue fever, chikungunya and zika. traditionally, control of ae. aegypti and other arthropod species has relied on the use of a relatively small diversity of chemical insecticides. however, wid ...201931671165
design of a repellent against aedes aegypti (diptera: culicidae) using in silico simulations with aaegobp1 protein.skin irritation has been reported to be the main adverse effect of excessive use of n,n-diethyl-m-toluamide (deet) and ethyl 3-acetyl(butyl)amino (ir3535) commercial repellents. therefore, there is an interest in alternatives of natural origin such as essential oils (eos) and major compounds, which have repellent effects but have no contraindications. the main purpose of the present study was to identify the repellent effect of selected terpenes on aedes aegypti linnaeus, 1762 (diptera: culicida ...201931670811
establishment of wmel wolbachia in aedes aegypti mosquitoes and reduction of local dengue transmission in cairns and surrounding locations in northern queensland, australia.background: the wmel strain of wolbachia has been successfully introduced into aedes aegypti mosquitoes and subsequently shown in laboratory studies to reduce transmission of a range of viruses including dengue, zika, chikungunya, yellow fever, and mayaro viruses that cause human disease. here we report the entomological and epidemiological outcomes of staged deployment of wolbachia across nearly all significant dengue transmission risk areas in australia. methods: the  wmel strain of  wolbachia ...201931667465
combined effect of the entomopathogenic fungus metarhizium robertsii and avermectins on the survival and immune response of aedes aegypti larvae.combination of insect pathogenic fungi and microbial metabolites is a prospective method for mosquito control. the effect of the entomopathogenic fungus metarhizium robertsii j.f. bischoff, s.a. rehner & humber and avermectins on the survival and physiological parameters of aedes aegypti (linnaeus, 1762) larvae (dopamine concentration, glutathione s-transferase (gst), nonspecific esterases (est), acid proteases, lysozyme-like, phenoloxidase (po) activities) was studied. it is shown that the comb ...201931667017
patterns of genetic divergence among populations of aedes aegypti l. (diptera: culicidae) in the southeastern usa.the yellow fever mosquito, aedes aegypti is a public health concern in the usa, especially in the wake of emergent diseases such as zika and chikungunya. aedes aegypti populations dwindled after the invasion of aedes albopictus in the 1980s and many populations were extirpated. however, in some areas ae. aegypti persisted in small populations and there are reports of recent resurgences of ae. aegypti in florida, louisiana, nevada and california. we assessed the population genetic structure of ae ...201931666113
gene expression profile of aedes aegypti females in courtship and mating.aedes aegypti is the most synanthropic and anthropophilic mosquito of culicidae. this species always cohabits with humans and is extremely opportunistic. vector dispersal is directly related to the ability of the females on successfully finding a mate in a generally patchy urban scenario. in the present work, we investigate transcriptional changes in ae. aegypti females during the courtship process and after mating. we observe a substantial alteration in gene expression triggered just upon conta ...201931664152
synergistic action of the transcription factors krüppel homolog 1 and hairy in juvenile hormone/methoprene-tolerant-mediated gene-repression in the mosquito aedes aegypti.arthropod-specific juvenile hormones control numerous essential functions in development and reproduction. in the dengue-fever mosquito aedes aegypti, in addition to its role in immature stages, juvenile hormone iii (jh) governs post-eclosion (pe) development in adult females, a phase required for competence acquisition for blood feeding and subsequent egg maturation. during pe, jh through its receptor methoprene-tolerant (met) regulate the expression of many genes, causing either activation or ...201931661489
combined sterile insect technique and incompatible insect technique: the first proof-of-concept to suppress aedes aegypti vector populations in semi-rural settings in thailand.important arboviral diseases, such as dengue, chikungunya, and zika virus infections, are transmitted mainly by the aedes aegypti vector. so far, controlling this vector species with current tools and strategies has not demonstrated sustainable and significant impacts. our main objective was to evaluate whether open field release of sterile males, produced from combining the sterile insect technique using radiation with the insect incompatible technique through wolbachia-induced incompatibility ...201931658265
congenital dengue in a saudi neonate: a case report.dengue fever is a mosquito-borne viral illness with 100 million new cases occurring worldwide. the vectors involved are mainly aedes aegypti and aedes albopictus. dengue infection is associated with maternal as well as fetal morbidities, like stillbirth, preterm birth, and low birth weight.201931658068
dengue fever - an update review and implications for nigeria, and similar countries.in the last five decades, dengue has emerged as one of the most important infectious diseases, following a 30-fold increase in global incidence throughout tropical and sub-tropical regions of the world. the actual numbers of dengue cases are under-reported and many cases are misclassified.201931656483
dna damage response and repair in perspective: aedes aegypti, drosophila melanogaster and homo sapiens.the maintenance of genomic integrity is the responsibility of a complex network, denominated the dna damage response (ddr), which controls the lesion detection and dna repair. the main repair pathways are base excision repair (ber), nucleotide excision repair (ner), mismatch repair (mmr), homologous recombination repair (hr) and non-homologous end joining repair (nhej). they correct double-strand breaks (dsb), single-strand breaks, mismatches and others, or when the damage is quite extensive and ...201931711518
potential of aedes albopictus and aedes aegypti (diptera: culicidae) to transmit yellow fever virus in urban areas in central africa.yellow fever (yf) remains a major public health issue in sub-saharan africa and south america, despite the availability of an effective vaccine. in africa, most yf outbreaks are reported in west africa. however, urban outbreaks occurred in 2016 in both angola and the democratic republic of congo (drc), and imported cases were reported in chinese workers coming back from africa. in central africa, cameroon and the republic of congo host a high proportion of non-vaccinated populations increasing t ...201931711378
properties, toxicity and current applications of the biolarvicide spinosad.characterized as a highly valuable bioactive natural product, spinosad is a pesticide with a complex chemical structure, composed of spinosyn a and d, molecules synthesized by the actinomycete saccharopolyspora spinosa. the larvicidal activity of spinosad was postulated to be a promising approach to combat crop pests and control species responsible to transmit mosquito-borne illness, including aedes aegypti. although initially deemed as relatively safe for non-target organisms and highly effecti ...201931709913
model to design insecticides against aedes aegypti using in silico and in vivo analysis of different pharmacological targets.compounds having insecticidal activity can be used to control aedes aegypti mosquitoes, a major worldwide vector, and several plants have a source of such molecules. a principal component analysis (pca) was carried out to determine the criterion to select larvicidal metabolites. the insecticidal activity of seven selected metabolites by pca was validated by determining its lethal concentrations 50 (lc50) by probit analysis. six of the seven evaluated molecules presented lc50 values <100 ppm. the ...201931707089
modeling temperature effects on population density of the dengue mosquito aedes aegypti.mosquito density plays an important role in the spread of mosquito-borne diseases such as dengue and zika. while it remains very challenging to estimate the density of mosquitoes, modelers have tried different methods to represent it in mathematical models. the goal of this paper is to investigate the various ways mosquito density has been quantified, as well as to propose a dynamical system model that includes the details of mosquito life stages leading to the adult population. we first discuss ...201931703421
aedes aegypti ir8a mutant female mosquitoes show increased attraction to standing water.the detection of water sources is crucial for insects such as mosquitoes to avoid desiccation and survive. in addition, mosquitoes use humidity cues to successfully navigate the environment to find a suitable oviposition site. previous studies have implicated some members of the ionotropic receptor family in humidity sensing by drosophila. here, we investigate if ir8a co-receptor mediates water detection in aedes aegypti mosquitoes. using a simple behavioral assay, we examined the attraction of ...201931700566
microbiota potentialized larvicidal action of imidazolium salts against aedes aegypti (diptera: culicidae).mosquitoes are important vectors of pathogens due to their blood feeding behavior. aedes aegypti (diptera: culicidae) transmits arboviruses, such as dengue, zika, and chikungunya. this species carries several bacteria that may be beneficial for its biological and physiological development. therefore, studying the response of its microbiota to chemical products could result in vector control. recently, imidazolium salts (is) were identified as effective ae. aegypti larvicides. considering the imp ...201931700056
estimation of the expected number of cases of microcephaly in brazil as a result of zika.in this paper we have adapted a delayed dengue model to zika. by assuming that the epidemic starts by a single infected individual entering a disease-free population at some initial time t0 we have used the least squares parameter estimation technique in r to estimate the initial time t0 using observed zika data from brazil as well as the transmission probabilities of zika in brazil between humans and mosquitoes and vice-versa. different values of aedes aegypti (a. aegypti) biting rate are used ...201931698665
identification of zika virus in immature phases of aedes aegypti and aedes albopictus: a surveillance strategy for outbreak anticipation.a progressive increase in the circulation of arboviruses in tropical countries has been observed, accounting for 700,000 yearly deaths in the world. the main objective of this article was to identify the presence of zika (zikv), dengue (denv), and chikungunya (chikv) viruses in immature stages of aedes aegypti and ae. albopictus. household collections of immature phases of the vectors were carried out in the years 2015 and 2016. a total of 2902 dwellings were visited and the rate of infestation ...201931721902
genomic epidemiology supports multiple introductions and cryptic transmission of zika virus in colombia.colombia was the second most affected country during the american zika virus (zikv) epidemic, with over 109,000 reported cases. despite the scale of the outbreak, limited genomic sequence data were available from colombia. we sought to sequence additional samples and use genomic epidemiology to describe zikv dynamics in colombia.201931718580
the influence of new surveillance data on predictive species distribution modeling of aedes aegypti and aedes albopictus in the united states.the recent emergence or reemergence of various vector-borne diseases makes the knowledge of disease vectors' presence and distribution of paramount concern for protecting national human and animal health. while several studies have modeled aedes aegypti or aedes albopictus distributions in the past five years, studies at a large scale can miss the complexities that contribute to a species' distribution. many localities in the united states have lacked or had sporadic surveillance conducted for t ...201931718099
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