TitleAbstractYear(sorted descending)
mosquito iridescent virus: new records from nature and infections using strelkovimermis spiculatus (mermithidae) as a vector under laboratory conditions.iridoviridae is a dna virus family that affects both vertebrates and invertebrates. immature aquatic stages of many dipteran species infected with iridovirus have been found in different places worldwide. the most represented genera of the culicidae family are aedes and psorophora. to date, sixteen species of aedes naturally infected with iridoviruses have been reported. moreover, there are four records for the genus psorophora, one for culiseta, and two for culex. in this paper, we report two n ...202031903519
aedes aegypti insecticide resistance underlies the success (and failure) of wolbachia population replacement.mosquitoes that carry wolbachia endosymbionts may help control the spread of arboviral diseases, such as dengue, zika and chikungunya. wolbachia frequencies systematically increase only when the frequency-dependent advantage due to cytoplasmic incompatibility exceeds frequency-independent costs, which may be intrinsic to the wolbachia and/or can be associated with the genetic background into which wolbachia are introduced. costs depend on field conditions such as the environmental pesticide load ...202031919396
sequencing of zikv genomes directly from ae. aegypti and cx. quinquefasciatus mosquitoes collected during the 2015-16 epidemics in recife.zika virus (zikv) is a negative sense rna virus from the flaviviridae family, which was relatively unknown until the first human epidemic in micronesia, in 2007. since its spread to french polynesia and the americas. recife, the capital of pernambuco state and epicenter of the zika epidemic in brazil, experienced a large number of microcephaly cases and other congenital abnormalities associated to the zikv infection from, 2015 to 16. evidences suggest that both aedes aegypti and culex quinquefas ...202031918041
development, larvicide activity, and toxicity in nontarget species of the croton linearis jacq essential oil this study, the essential oil (eo) from leaves of croton linearis jacq was extracted and characterized by gc/ms. the eo hydrophilic-lipophilic balance required (rhlb) for nanoemulsion (ne) development was determined by the griffin' method. for evaluating the larvicidal effect against aedes aegypti, the preparation process of ne was optimized, using a central composite design. it was also evaluated the possible toxic effect of ne in nontarget species. the leaves of c. linearis contain 1.50% of ...202031916152
chemosensation: hate mosquitoes? peel beetroots!finding the right lure for trapping pest insects is difficult. the typical smell of rain and humid soil, geosmin, now turns out to be a strong attractant for the yellow fever mosquito aedes aegypti.202031910367
geographic shifts in the bioclimatic suitability for aedes aegypti under climate change scenarios in colombia.the dengue, chikungunya and zika viruses are arboviruses predominantly transmitted to humans through the bite of the female mosquito aedes aegypti. currently, the vector represents a potential epidemiological risk in several latin american and pacific countries. however, little is known about the geographical distribution and bioclimatic suitability of this mosquito in the projected climate change scenarios in colombia. using a species distribution model of maximum entropy (maxent) based on pres ...202031909268
surveillance for zika in mexico: naturally infected mosquitoes in urban and semi-urban areas.zika cases have been reported in 29 out of the 32 states of mexico. information regarding which mosquito species might be driving zika virus transmission/maintenance in nature must be regularly updated. from january 2017 to november 2018, mosquitoes were collected indoors and outdoors using the cdc backpack aspirator in urban and semi-urban areas with evidence of mosquito-borne disease transmission. 3873 mosquito pools were tested for zika infection using the cdc trioplex real-time rt-pcr. for e ...202031902313
cytotoxic and larvicidal activities of stemona alkaloids from the aerial parts and roots of stemona curtisii hook.f.a new stemona alkaloid glycoside derivative, 6-hydroxy-5,6-seco-stemocurtisinoside (4), was isolated from the ethanolic extract of the aerial parts of stemona curtisii hook.f., together with stemocurtisine (1), (11z)-1',2'-didehydrostemofoline (2) and 6-hydroxy-5,6-seco-stemocurtisine (3). whereas, stemocurtisine (1), stemocurtisinol (5) and oxyprotostemonine (6) were isolated from the roots. their structures were elucidated using 1d and 2d nmr spectroscopy as well as ms experiments. the extract ...202031899956
discovery of 1,2,4-oxadiazole derivatives as a novel class of noncompetitive inhibitors of 3-hydroxykynurenine transaminase (hkt) from aedes aegypti.the mosquito aedes aegypti is the vector of arboviruses such as zika, chikungunya, dengue and yellow fever. these infectious diseases have a major impact on public health. the unavailability of effective vaccines or drugs to prevent or treat most of these diseases makes vector control the main form of prevention. one strategy to promote mosquito population control is the use of synthetic insecticides to inhibit key enzymes in the metabolic pathway of these insects, particularly during larval sta ...202031864777
the olfactory basis of orchid pollination by mosquitoes.mosquitoes are important vectors of disease and require sources of carbohydrates for reproduction and survival. unlike host-related behaviors of mosquitoes, comparatively less is understood about the mechanisms involved in nectar-feeding decisions, or how this sensory information is processed in the mosquito brain. here we show that aedes spp. mosquitoes, including aedes aegypti, are effective pollinators of the platanthera obtusata orchid, and demonstrate this mutualism is mediated by the orchi ...202031871198
geosmin attracts aedes aegypti mosquitoes to oviposition sites.geosmin is one of the most recognizable and common microbial smells on the planet. some insects, like mosquitoes, require microbial-rich environments for their progeny, whereas for other insects such microbes may prove dangerous. in the vinegar fly drosophila melanogaster, geosmin is decoded in a remarkably precise fashion and induces aversion, presumably signaling the presence of harmful microbes [1]. we have here investigated the effect of geosmin on the behavior of the yellow fever mosquito a ...202031839454
function of aedes aegypti galectin-6 in modulation of cry11aa toxicity.galectins are a family of β-galactoside binding proteins, and insect galectins play a role in immune responses and may also affect cry toxin activity. in this study, we aimed to further understand the function and molecular mechanism of aedes aegypti galectin-6 in modulation of cry11aa toxicity. a. aegypti galectin-6 was cloned, and the recombinant galectin-6 was expressed and purified. bioassays indicated that galectin-6 could reduce the toxicity of cry11aa, protecting a. aegypti larvae. to det ...202031836060
annona muricata: an alternate mosquito control agent with special reference to inhibition of detoxifying enzymes in aedes aegypti.this study was aimed to investigate an effectual level of annona muricata (soursop) extracts on mosquito vectors namely, aedes aegypti, anopheles stephensi and culex quinquefasciatus. the toxicity study on non-target organism and other important biochemical marker enzymes to find and illustrate the exact mechanism of specific enzymes responsible for detoxifying allelochemicals. among the various soursop seed kernel extracts tested for larvicidal activity, the 0.9% saline extract exhibited maximu ...202031816498
glycolytic profile shift and antioxidant triggering in symbiont-free and h2o2-resistant strigomonas culicis.during their life cycle, trypanosomatids are exposed to stress conditions and adapt their energy and antioxidant metabolism to colonize their hosts. strigomonas culicis is a monoxenous protist found in invertebrates with an endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. our research group previously generated a wild-type h2o2-resistant (wtr) strain that showed improved mitochondrial metabolism and antioxidant defenses, which led to higher rates of ...202031760093
evolution of the innate and adaptive immune response in women with acute zika virus infection.zika virus (zikv) is a flavivirus that is closely related to other human pathogens, such as dengue virus (denv)1. primary transmission usually involves aedes aegypti, which has expanded its distribution range considerably2, although rarer infection routes, including mother-to-fetus transmission, sexual contact and blood transfusion, have also been observed3-7. primary zikv infection is usually asymptomatic or mild in adults, with quickly resolved blood viraemia, but zikv might persist for months ...202031792427
measuring the host-seeking ability of aedes aegypti destined for field seeking is an essential process in mosquito reproduction. field releases of modified mosquitoes for population replacement rely on successful host seeking by female mosquitoes, but host-seeking ability is rarely tested in a realistic context. we tested the host-seeking ability of female aedes aegypti mosquitoes using a semi-field system. females with different wolbachia infection types (wmel-, walbb-infected, and uninfected) or from different origins (laboratory and field) were released at ...202031769394
the role of lulopat amino acid/proton symporters in midgut alkalinization in the sandfly lutzomyia longipalpis (diptera - psychodidae).in lutzomyia longipalpis females, which are the main vectors of leishmania infantum in the americas, hematophagy is crucial for ovary development. the control of ph in the midgut during blood digestion is important to the functioning of the digestive enzymes, which release amino acids in the luminal compartment that are then transported through the enterocytes to the hemolymph for delivery to the ovary and other organs. in the present work, we investigated transport systems known as lulopats tha ...202031715141
molecular detection of dengue virus in mosquitoes as an early indicator to aid in the prevention of human infection in endemic areas.human cases of dengue virus based on the national dengue control plan were compared with the molecular detection of the dengue virus in trapped mosquitoes, verifying the prediction and efficacy potentials of vector control between the two methodologies in a city with three endemic frontiers. molecular detection of dengue virus in trapped mosquitoes was significantly higher than in human cases (p = 0.0435). thus, molecular detection could be used as an early indicator to help prevent more human c ...202031697613
phytochemical analysis of rhazya stricta extract and its use in fabrication of silver nanoparticles effective against mosquito vectors and microbial pathogens.worldwide, billions of people are at risk from viruses, parasites and bacteria transmitted by mosquitoes, ticks, fleas and other vectors. over exploitation of chemical pesticides to overcome the mosquito borne diseases led to detrimental effects on environment and human health. the present research aims to explore bio-fabrication of metal silver nanomaterials using rhazya stricta extract against deadly mosquito vectors and microbial pathogens. the phytochemical profile of the r. stricta extracts ...202031655455
insecticidal and mosquito repellent efficacy of the essential oils from stem bark and wood of hazomalania voyronii.the use of hazomalania voyronii, popularly known as hazomalana, to repel mosquitoes and resist against insect attacks is handed down from generation to generation in madagascar. in the present study, we investigated the ability of the essential oils (eos) obtained from the stem wood, fresh and dry bark of h. voyronii to keep important mosquito vectors (aedes aegypti and culex quinquefasciatus) away, as well as their toxicity on three insect species of agricultural and public health importance (c ...202031654797
control of the 2018-2019 dengue fever outbreak in oman: a country previously without local december 2018, routine surveillance identified an autochthonous outbreak of dengue fever in muscat governorate, oman, a region previously free of dengue fever.202031639520
detection of fluorescent powders and their effect on survival and recapture of aedes aegypti (diptera: culicidae).the use of insect markers, such as fluorescent powders, is a useful tool for studying ecological and epidemiological questions. evaluating their effect on vectors of human disease agents, such as the invasive mosquito vector aedes aegypti (linnaeus), is crucial for their practical and reliable use, especially in parameters linked to the risk of disease transmission such as adult survival, dispersal, and host-seeking. seven fluorescent powders (hercules radiant, dayglo (dg), risk reactor (rr), an ...202031587060
towards the optimization of botanical insecticides research: aedes aegypti larvicidal natural products in french guiana.natural products have proven to be an immeasurable source of bioactive compounds. the exceptional biodiversity encountered in amazonia, alongside a rich entomofauna and frequent interactions with various herbivores is the crucible of a promising chemodiversity. this prompted us to search for novel botanical insecticides in french guiana. as this french overseas department faces severe issues linked to insects, notably the strong incidence of vector-borne infectious diseases, we decided to focus ...202031539525
distribution and frequency of the kdr mutation v410l in natural populations of aedes aegypti (l.) (diptera: culicidae) from eastern and southern mexico.aedes aegypti (l.) is the primary vector of the viruses that cause dengue, zika, and chikungunya, for which effective vaccines and drugs are still lacking. current strategies for suppressing arbovirus outbreaks based on insecticide use pose a challenge because of the rapid increase in resistance. the widespread and excessive use of pyrethroid-based insecticides has created a large selection pressure for a kdr-type resistance, caused by mutations in the para gene of the voltage-gated sodium chann ...202031504686
mapping aedes aegypti (diptera: culicidae) and aedes albopictus vector mosquito distribution in brownsville, tx.aedes mosquitoes are vectors of several emerging diseases and are spreading worldwide. we investigated the spatiotemporal dynamics of aedes aegypti (linnaeus) and aedes albopictus (skuse) mosquito trap captures in brownsville, tx, using high-resolution land cover, socioeconomic, and meteorological data. we modeled mosquito trap counts using a bayesian hierarchical mixed-effects model with spatially correlated residuals. the models indicated an inverse relationship between temperature and mosquit ...202031400202
reduced effectiveness of repellents in a pyrethroid-resistant strain of aedes aegypti (diptera: culicidae) and its correlation with olfactory sensitivity.the mosquito, aedes aegypti (diptera: culicidae), is a vector of dengue fever, zika, chikungunya, and yellow fever viruses, and in many areas possesses significant levels of resistance to pyrethroids. behavioral performance was assessed in 15, 30, and 60 min exposures in a high throughput vapor phase spatial repellency assay to three contact repellent standards: n,n-diethyl-3-methylbenzamide (deet), ethyl 3-[acetyl(butyl)amino] propanoate (ir3535), and 2-undecanone, as well as pyrethrum extract, ...202031338960
conservation of the voltage-sensitive sodium channel protein within the insecta.the voltage-sensitive sodium channel (vssc) is essential for the generation and propagation of action potentials. vssc kinetics can be modified by producing different splice variants. the functionality of vssc depends on features such as the voltage sensors, the selectivity filter and the inactivation loop. mutations in vssc conferring resistance to pyrethroid insecticides are known as knockdown resistance (kdr). we analysed the conservation of vssc in both a broad scope and a narrow scope by th ...202031206812
adaptation to temperate climates: evidence of photoperiod-induced embryonic dormancy in aedes aegypti in south america.dormancy is a developmental arrest in arthropods, in response to unfavorable conditions in temporally varying environments. in aedes aegypti, the supposed inability of eggs to inhibit hatching has been used to explain the restriction of this species to tropical and subtropical regions. however, the geographic range of ae. aegypti is constantly expanding towards temperate regions. thus, the aim of the present study was to assess the ability of ae. aegypti individuals from a temperate region (buen ...202031125550
larval exposure to bacteria modulates arbovirus infection and immune gene expression in adult aedes we have investigated whether bacterial challenges to larval stages of aedes aegypti can influence the adults' immune and vector competence for dengue and zika viruses. we show that larval exposure to live bacillus thuringiensis berliner and enterobacter ludwigii can result in the modulation of virus infection at the adult stage in the absence of bacterial carry-over between the two developmental stages. we observed a significant reduction in virus infection intensity in the mosquitoes expos ...202031726064
the global expansion of dengue: how aedes aegypti mosquitoes enabled the first pandemic arbovirus.dengue is an emerging viral disease principally transmitted by the aedes (stegomyia) aegypti mosquito. it is one of the fastest-growing global infectious diseases, with 100-400 million new infections a year, and is now entrenched in a growing number of tropical megacities. behind this rapid rise is the simple adaptation of ae. aegypti to a new entomological niche carved out by human habitation. this review describes the expansion of dengue and explores how key changes in the ecology of ae. aegyp ...202031594415
chikungunya virus: role of vectors in emergence from enzootic cycles.chikungunya virus (chikv), a re-emerging mosquito-borne arbovirus, has caused millions of cases of severe, often chronic arthralgia during recent outbreaks. in africa, circulation in sylvatic, enzootic cycles involves several species of arboreal mosquito vectors that transmit among diverse nonhuman primates and possibly other amplifying hosts. most disease occurs when chikv emerges into a human-amplified cycle involving aedes aegypti and sometimes aedes albopictus transmission and extensive spre ...202031594410
assessment of hymenoptera and non-hymenoptera insect bite and sting allergy among patients of tropical region of west bengal, india.west bengal, india, is inhabited by abundance and variety of insects that triggers sensitization in some humans to inhalant allergens and/or insect stings/venoms. lack of research on this topic prevented accurate diagnosis and proper follow-up treatments to patients suffering from insect-induced allergies. the aim of our study was to identify the allergy-causing insects and evaluate resulting sensitization among a study population in west bengal, india. the skin prick test (spt) evaluated sensit ...202031228205
excito-repellency activity of andrographis paniculata (lamiales: acanthaceae) against colonized mosquitoes.excito-repellency activity of plant extracts have been increasingly studied as mosquito repellents. in this study, the crude extract of andrographis paniculata was evaluated for its noncontact repellency, contact excitation (irritancy + repellency), and knockdown/toxicity response against five colonized mosquitoes; aedes aegypti (l.), aedes albopictus (skuse), anopheles dirus peyton & harrison, anopheles epiroticus linton & harbach, and culex quinquefasciatus say (diptera: culicidae) using an ex ...202031550361
phylogeography and invasion history of aedes aegypti, the dengue and zika mosquito vector in cape verde islands (west africa).aedes-borne arboviruses have spread globally with outbreaks of vast impact on human populations and health systems. the west african archipelago of cape verde had its first outbreak of dengue in 2009, at the time the largest recorded in africa, and was one of the few african countries affected by the zika virus epidemic. aedes aegypti was the mosquito vector involved in both outbreaks. we performed a phylogeographic and population genetics study of a. aegypti in cape verde in order to infer the ...201931548858
panophthalmitis associated with scleral necrosis in dengue hemorrhagic fever.dengue is a mosquito-borne flavivirus disease affecting humans. the aedes aegypti mosquito spreads it. ophthalmic manifestations of dengue range from subconjunctival hemorrhage to optic neuropathy. panophthalmitis in dengue fever is a rare finding. we report a case of a 22-year-old male having dengue fever, who presented with pain, redness, swelling and loss of vision in his right eye. he was diagnosed as panophthalmitis with subretinal hemorrhage and required right eye evisceration.201931546563
the efficiency of a new automated mosquito larval counter and its impact on larval achieve consistent and standardized rearing for mosquito immature stages, it is crucial to control the initial number of larvae present in each larval tray. in addition, maintaining an optimal and synchronized development rate of larvae is essential to maximize the pupal production and optimize male sorting in a mass-rearing setting. manual counting is labor intensive, time consuming and error prone. therefore, this study aimed to investigate the use of a customized automated counter for the ...201931092868
infection of aedes albopictus mosquito c6/36 cells with the wmelpop strain of wolbachia modulates dengue virus-induced host cellular transcripts and induces critical sequence alterations in the dengue viral genome.dengue virus (denv) causes frequent epidemics infecting ∼390 million people annually in over 100 countries. there are no approved vaccines or antiviral drugs for treatment of infected patients. however, there is a novel approach to control denv transmission by the mosquito vectors, aedes aegypti and aedes albopictus, using the wolbachia symbiont. the wmelpop strain of wolbachia suppresses denv transmission and shortens the mosquito life span. however, the underlying mechanism is poorly understoo ...201931092581
microencapsulation of citrus grandis peel oil using interfacial precipitation chemistry technique for repellent application.essential oil of citrus family plant is known to have repellent effect against mosquito. unfortunately, due to its high volatility effect, its repellency effect was compromised. the incorporation of essential oil in a microencapsulation formulation has been shown to help improve the stability and potency of the repellent. in this study, citrus grandis peel oil (cgpo) was encapsulated by using the interfacial precipitation chemistry technique. the microencapsulated cgpo was then formulated into l ...201931089355
larvicidal, adulticidal, and oviposition-deterrent activity of piper betle l. essential oil to aedes aegypti.aedes aegypti is a primary vector of many arthropod-borne diseases. one of the diseases, dengue fever, is an endemic disease in indonesia causing high mortalities for decades. there are no preventive and specific treatments for dengue so far. therefore, prevention of this disease largely depends on the mosquito control. since resistance to chemical insecticides occurred worldwide, the study on alternate and new mosquito insecticides are mandatory. this study aimed to demonstrate the effect of es ...201931089304
efficacy of the in2care® auto-dissemination device for reducing dengue transmission: study protocol for a parallel, two-armed cluster randomised trial in the philippines.mosquito-borne viruses are imposing an ever increasing health burden worldwide. in addition to the recent zika and chikungunya virus epidemics, dengue viruses have become the fastest growing problem with a 40-fold increase in the number of reported cases over the past five decades. current mosquito control techniques involving larval source reduction, larviciding, and space spray of adulticides are costly, laborious, and of debatable efficacy. there remains an urgent need for the development of ...201931088515
linking nutrient stoichiometry to zika virus transmission in a quality and quantity serve as the basis for cycling of key chemical elements in trophic interactions; yet the role of nutrient stoichiometry in shaping host-pathogen interactions is under appreciated. most of the emergent mosquito-borne viruses affecting human health are transmitted by mosquitoes that inhabit container systems during their immature stages, where allochthonous input of detritus serves as the basal nutrients. quantity and type of detritus (animal and plant) were manipulated i ...201931227906
lichen parmelia sulcata mediated synthesis of gold nanoparticles: an eco-friendly tool against anopheles stephensi and aedes aegypti.the gold nanoparticles (aunps) were synthesized using the lichen parmelia sulcata extract (pse) and characterized. the peaks of ultraviolet spectrophotometer and fourier transmission infrared confirmed the formation of nanoparticles and the bioactive compounds of the lichen being responsible for reducing and capping of the particles. the face-centered cubic particles were determined by xrd peaks at 111, 200, 220, and 311. the elemental composition and spherical shape of aunps were confirmed by e ...201931218582
how do trait-mediated non-lethal effects of predation affect population-level performance of mosquitoes?non-lethal, trait-mediated effects of predation impact prey behavior and life-history traits. studying how these effects in turn influence prey demography is crucial to understand prey life-history evolution. mosquitoes are important vectors that claim several million lives every year worldwide by transmitting a range of pathogens. several ecological factors affect life-history traits of both larval and adult mosquitoes, creating effects that cascade to population-level consequences. few studies ...201931218216
community composition and year-round abundance of vector species of mosquitoes make miami-dade county, florida a receptive gateway for arbovirus entry to the united states.vector-borne diseases are a heavy burden to human-kind. global warming and urbanization have a significant impact on vector-borne disease transmission, resulting in more severe outbreaks, and outbreaks in formerly non-endemic areas. miami-dade county, florida was the most affected area in the continental united states during the 2016 zika virus outbreak. miami is an important gateway and has suitable conditions for mosquitoes year-round. therefore, it was critical to establish and validate a sur ...201931217547
development of cs-tpp-dsrna nanoparticles to enhance rnai efficiency in the yellow fever mosquito, aedes aegypti.mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. vector control is one of the most important approaches used in reducing the incidence of these diseases. however, increasing mosquito resistance to chemical insecticides presents challenges to this approach. therefore, new strategies are necessary to develop the next generation vector control methods. because of the target specificity of dsrna, rnai-based control measures are ...201931217512
mosquitocidal chips containing the insect growth regulator pyriproxyfen for control of aedes aegypti (diptera: culicidae).aedes aegypti were exposed to water treated with mosquitocidal chips containing the insecticide pyriproxyfen in a polymer formulation. chips were tested under different conditions; different water volumes, in containers made of different material, and in water with different levels of organic matter. treated chips caused 100% mortality of ae. aegypti during their pupal stage independent of size or type of container, and the mount of organic matter contained in the water to which the mosquito lar ...201931216627
oviposition preferences of the mosquito aedes aegypti linnaeus, 1762 (culicidae): an urban environment bioassay.the establishment of an invasive species depends on reproductive success and dispersion capability in the new environment. one of the striking examples of invasion in urban environments is the mosquito aedes aegypti linnaeus, 1762 (culicidae). the success of this species is primarily attributed to its ability to colonize urban environments, and some of the important adaptive strategies associated with this ability is the preference for humans as a blood source and intense occupation of residenti ...201931213206
inferring who-infected-whom-where in the 2016 zika outbreak in singapore-a spatio-temporal model.singapore experienced its first known zika outbreak in 2016. given the lack of herd immunity, the suitability of the climate for pathogen transmission, and the year-round presence of the vector- aedes aegypti-zika had the potential to become endemic, like dengue. guillain-barré syndrome and microcephaly are severe complications associated elsewhere with zika and the risk of these complications makes understanding its spread imperative. we investigated the spatio-temporal spread of locally transm ...201931213175
asymptomatic dengue virus infections, cambodia, 2012-2013.we investigated dengue virus (denv) and asymptomatic denv infections in rural villages of kampong cham province, cambodia, during 2012 and 2013. we conducted perifocal investigations in and around households for 149 denv index cases identified through hospital and village surveillance. we tested participants 0.5-30 years of age by using nonstructural 1 rapid tests and confirmed denv infections using quantitative reverse transcription pcr or nonstructural 1-capture elisa. we used multivariable po ...201931211672
live calcium imaging of aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies.the mosquito aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. in particular, compared to ...201931208328
the difference of sve-cadherin levels between dengue hemorrhagic fever patients with shock and without shock.dengue virus infection is an infectious disease caused by the dengue virus and transmitted by the aedes aegypti mosquito. dengue virus (den-v) consists of 4 serotypes, namely den-1, den-2, den-3, and den-4. the most feared result of dhf is death. death in children is caused by hypovolemic shock due to plasma leakage from intravascular to extravascular space due to endothelial dysfunction.201931592274
a peridomestic aedes malayensis population in singapore can transmit yellow fever virus.the case-fatality rate of yellow fever virus (yfv) is one of the highest among arthropod-borne viruses (arboviruses). although historically, the asia-pacific region has remained free of yfv, the risk of introduction has never been higher due to the increasing influx of people from endemic regions and the recent outbreaks in africa and south america. singapore is a global hub for trade and tourism and therefore at high risk for yfv introduction. effective control of the main domestic mosquito vec ...201931589616
identification of zika virus in immature phases of aedes aegypti and aedes albopictus: a surveillance strategy for outbreak anticipation.a progressive increase in the circulation of arboviruses in tropical countries has been observed, accounting for 700,000 yearly deaths in the world. the main objective of this article was to identify the presence of zika (zikv), dengue (denv), and chikungunya (chikv) viruses in immature stages of aedes aegypti and ae. albopictus. household collections of immature phases of the vectors were carried out in the years 2015 and 2016. a total of 2902 dwellings were visited and the rate of infestation ...201931721902
genomic epidemiology supports multiple introductions and cryptic transmission of zika virus in colombia.colombia was the second most affected country during the american zika virus (zikv) epidemic, with over 109,000 reported cases. despite the scale of the outbreak, limited genomic sequence data were available from colombia. we sought to sequence additional samples and use genomic epidemiology to describe zikv dynamics in colombia.201931718580
the influence of new surveillance data on predictive species distribution modeling of aedes aegypti and aedes albopictus in the united states.the recent emergence or reemergence of various vector-borne diseases makes the knowledge of disease vectors' presence and distribution of paramount concern for protecting national human and animal health. while several studies have modeled aedes aegypti or aedes albopictus distributions in the past five years, studies at a large scale can miss the complexities that contribute to a species' distribution. many localities in the united states have lacked or had sporadic surveillance conducted for t ...201931718099
chemical compositions and mosquito larvicidal activities of essential oils from piper species growing wild in central vietnam.mosquitoes are the deadliest animals on earth and are the vectors of several neglected tropical diseases. recently, essential oils have emerged as potential renewable, cost-effective, and environmentally benign alternatives to synthetic pesticides for control of mosquitoes. in this work, thirteen species of piper were collected from different areas of central vietnam. the essential oils were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. the essential oils we ...201931717867
resistance status of aedes aegypti to deltamethrin, malathion, and temephos in ecuador, the status of insecticide resistance for aedes aegypti, the principal arboviral vector in the country, has not been previously evaluated. the aim of this research was to describe the resistance status of ae. aegypti to the principal insecticides used for vector control in provinces with high reports of arboviral clinical cases. this was a descriptive study performed on ae. aegypti collected from 2016 to 2017 in 14 localities of ecuador. the larvae were reared and tested using bioassa ...201931442135
a survey for aedes aegypti in delaware and the virus-positive pool rates of aedes albopictus and aedes triseriatus for west nile and zika viruses.the introduction of zika virus to the usa in 2015 engendered heightened interest in its known vectors. aedes aegypti is the primary vector, with ae. albopictus considered a potential secondary vector, together with several other possible marginal vectors. in delaware, ae. aegypti has been collected rarely, but no breeding populations were detected during past intensive statewide surveillance efforts. however, there is an abundance of ae. albopictus statewide. both species are container breeders ...201931442131
field efficacy trials of aerial ultra-low-volume application of insecticides against caged aedes aegypti in mexico.we evaluated the efficacy of aerial ultra-low-volume (ulv) insecticide spraying in field bioassays with caged aedes aegypti in may 2017 in puerto vallarta, jalisco, mexico. the insecticides tested included an organophosphate (mosquitocida uno ulv) and a neonicotinoid-pyrethroid combination (cielo). two ae. aegypti populations were evaluated: a field pyrethroid-resistant local strain (puerto vallarta) and an insecticide-susceptible laboratory strain (new orleans). knockdown after 1 h by both prod ...201931442128
evaluation and comparison of spray equipment for indoor residual spraying.the world health organization (who) has recently recommended indoor residual spraying (irs) as part of a vector control strategy to combat aedes-borne diseases, including dengue, chikungunya, and zika viruses. hand compression sprayers have been used in malaria prevention and control programs worldwide since the 1950s and are a standard for irs application. however, there are technological advances that should be considered to improve irs application (e.g., flow-control valves, rechargeable-batt ...201931442127
semi-field evaluation of modified 00zzzero® traps with sticky paper to increase the collection efficacy of gravid aedes aegypti.the 00zzzero® trap (0z) is a small black plastic container with permethrin and pyriproxyfen, designed and marketed to both attract and kill gravid aedes mosquitoes (unmodified trap ([umt] or modified trap [mt]). the 0z trap without the insecticide was modified with addition of a sticky paper, coated with an adhesive glue (mt) and evaluated for collection efficacy of released gravid mosquito, aedes aegypti (l.) in outdoor screened enclosures in st. augustine, in northeastern florida. the mean num ...201931442126
influence of sex ratio and density on the comparative repellency of ir3535® against caged aedes, anopheles, and culex mosquitoes.studies on topical repellent efficacy conducted with caged mosquitoes in the laboratory are important to both the development and regulation of insect repellents. guidelines for laboratory studies stipulate specific densities, sex ratios, and biting rates, whereas those for field studies are governmentally required before a promising repellent can be registered for human use. these protocols stipulate minimum biting rates alone. relatively little is known, however, about the influence of mosquit ...201931441773
dynamics and diversity of bacteria associated with the disease vectors aedes aegypti and aedes albopictus.aedes aegypti and aedes albopictus develop in the same aquatic sites where they encounter microorganisms that influence their life history and capacity to transmit human arboviruses. some bacteria such as wolbachia are currently being considered for the control of dengue, chikungunya and zika. yet little is known about the dynamics and diversity of aedes-associated bacteria, including larval habitat features that shape their tempo-spatial distribution. we applied large-scale 16s rrna amplicon se ...201931434963
systematic identification and characterization of aedes aegypti long noncoding rnas (lncrnas).long noncoding rnas (lncrnas) play diverse roles in biological processes. aedes aegypti (ae. aegypti), a blood-sucking mosquito, is the principal vector responsible for replication and transmission of arboviruses including dengue, zika, and chikungunya virus. systematic identification and developmental characterisation of ae. aegypti lncrnas are still limited. we performed genome-wide identification of lncrnas, followed by developmental profiling of lncrna in ae. aegypti. we identified a total o ...201931434910
copper(ii)-binding induces a unique polyproline type ii helical structure within the ion-binding segment in the intrinsically disordered f-domain of ecdysteroid receptor from aedes aegypti.reproduction of the dominant vector of zika and dengue diseases, aedes aegypti mosquito, is controlled by an active heterodimer complex composed of the 20-hydroxyecdysone receptor (ecr) and ultraspiracle protein. although a. aegypti ecr shares the structural and functional organization with other nuclear receptors, its c-terminus has an additional long f domain (aafecr). recently, we showed that the full length aafecr is intrinsically disordered with the ability to specifically bind divalent met ...201931433630
identification of anti-flaviviral drugs with mosquitocidal and anti-zika virus activity in aedes aegypti.zika virus (zikv), an emerging arbovirus belonging to the genus flavivirus, is transmitted by aedes mosquitoes. zikv infection can cause microcephaly of newborn babies and guillain-barré syndrome in adults. because no licensed vaccine or specific antiviral treatment is available for zikv infection, the most commonly used approach to control the spread of zikv is suppression of the mosquito vector population. a novel proposed strategy to block arthropod virus (arbovirus) transmission is based on ...201931430351
detecting wmel wolbachia in field-collected aedes aegypti mosquitoes using loop-mediated isothermal amplification (lamp).the world mosquito program uses wolbachia pipientis for the biocontrol of arboviruses transmitted by aedes aegypti mosquitoes. diagnostic testing for wolbachia in laboratory colonies and in field-caught mosquito populations has typically employed pcr. new, simpler methods to diagnose wolbachia infection in mosquitoes are required for large-scale operational use.201931416478
the major oligosaccharyl transferase complex genes are not involved in dengue virus replication in aedes aegypti mosquitoes.replication of the dengue virus (denv) genome occurs in a vesicle in the endoplasmic reticulum by a complex of host and viral proteins. two host proteins, stt3a and stt3b, as members of the oligosaccharyl transferase complex, have been implicated in playing structural roles in the vesicle in mammalian cells, and the absence of these proteins has been shown to decrease denv replication. aedes aegypti is the main vector of the virus and has been used previously as a model organism to study mosquit ...201931414286
antibiotic resistant bacteria and commensal fungi are common and conserved in the mosquito microbiome.the emerging and increasing prevalence of bacterial antibiotic resistance is a significant public health challenge. to begin to tackle this problem, it will be critical to not only understand the origins of this resistance but also document environmental reservoirs of antibiotic resistance. in this study we investigated the possibility that both colony and field caught mosquitoes could harbor antibiotic resistant bacteria. specifically, we characterized the antibiotic resistant bacterial populat ...201931412044
midgut barriers prevent the replication and dissemination of the yellow fever vaccine in aedes be transmitted to vertebrate hosts via the saliva of their vectors, arthropod-borne viruses have to cross several barriers in the mosquito body, including the midgut infection and escape barriers. yellow fever virus (yfv) belongs to the genus flavivirus, which includes human viruses transmitted by aedes mosquitoes, such as dengue and zika viruses. the live-attenuated yfv-17d vaccine has been used safely and efficiently on a large scale since the end of world war ii. early studies have shown, ...201931412040
nature of complex network of dengue epidemic as a scale-free network.dengue epidemic is a dynamic and complex phenomenon that has gained considerable attention due to its injurious effects. the focus of this study is to statically analyze the nature of the dengue epidemic network in terms of whether it follows the features of a scale-free network or a random network.201931406610
geometric morphometric analysis of the effect of temperature on wing size and shape in aedes albopictus.wing geometry helps to identify mosquito species, even cryptic ones. on the other hand, temperature has a well-known effect on insect metric properties. can such effects blur the taxonomic signal embedded in the wing? two strains of aedes albopictus (laboratory and field strain) were examined under three different rearing temperatures (26, 30 and 33 °c) using landmark- and outline-based morphometric approaches. the wings of each experimental line were compared with aedes aegypti. both approaches ...201931125148
new records of aedes aegypti and aedes albopictus in eight texas counties, u.s.a. 201931124239
spatial repellency and other effects of transfluthrin and linalool on aedes aegypti and aedes the present study, the effects of two spatial repellents (sr) were determined for aedes aegypti and ae albopictus, the main vectors of dengue, chikungunya, and zika fever. the modular high-throughput screening system (hitss) was used to evaluate the response of both species to transfluthrin and linalool sr at different concentrations. the highest spatial repellency results for ae. aegypti were obtained by transfluthrin to 0.001% with 37.50 ± 4.33%, and for linalool to 10% with 77.50 ± 3.90%. ...201931124222
diet drugs trick mosquitoes into feeling full.after a bloodmeal, aedesaegypti mosquitoes lose interest in bloodfeeding. duvall et al. (cell 2019;176:687-701) determined that the neuropeptide y (npy)-like receptor 7 (npylr7) controls mosquito satiety and also identified six npylr7 drug targets that suppress biting. this work highlights an innovative approach in vector control linking insect behavior to drug discovery.201931122765
excito-repellent activity of β-caryophyllene oxide against aedes aegypti and anopheles irritant and non-contact repellent activities of β-caryophyllene oxide were evaluated against laboratory strains of female aedes aegypti (usda strain), a major arbovirus vector and anopheles minimus (ku strain), a major malaria parasite vector, compared with the synthetic repellent deet, using an excito-repellency test system. β-caryophyllene oxide and deet were tested at concentrations of 0.1, 0.25, 0.5 and 1.0% (v/v). anopheles minimus was found to be more sensitive to β-caryophyllene ...201931121148
identification of molecular determinants of resistance to pyrethroid insecticides in aedes aegypti (diptera: culicidae) populations in california, usa.the first breeding populations of aedes aegypti (linnaeus) were identified in california in 2013, and have since been detected in 13 counties. recent studies suggest two introductions likely occurred, with genetically distinct populations in the central and southern regions of the state. given the threat of dengue, chikungunya, and zika virus transmission, it is imperative to understand if these populations harbor genes that could confer resistance to pyrethrin-based insecticides, known as pyret ...201931121042
uncertainty analysis of species distribution models.the maximum entropy model, a commonly used species distribution model (sdm) normally combines observations of the species occurrence with environmental information to predict the geographic distributions of animal or plant species. however, it only produces point estimates for the probability of species existence. to understand the uncertainty of the point estimates, we analytically derived the variance of the outputs of the maximum entropy model from the variance of the input. we applied the an ...201931120909
insecticide resistance levels and mechanisms in aedes aegypti populations in and around ouagadougou, burkina faso.recent outbreaks of dengue and other aedes aegypti-borne arboviruses highlight the importance of a rapid response for effective vector control. data on insecticide resistance and underlying mechanisms are essential for outbreak preparedness, but are sparse in much of africa. we investigated the levels and heterogeneity of insecticide resistance and mechanisms of ae. aegypti from contrasting settings within and around ouagadougou, burkina faso.201931120874
topological qsar modelling of carboxamides repellent activity to aedes aegypti.aedes aegypti vector control is of paramount importance owing to the damages induced by the various severe diseases that these insects may transmit, and the increasing risk of important outbreaks of these pathologies. search for new chemicals efficient against aedes aegypti, and devoid of side-effects, which have been associated to the currently most used active substance i. e. n,n-diethyl-m-toluamide (deet), is therefore an important issue. in this context, we developed various quantitative str ...201931120598
entomological efficacy of aerial ultra-low volume insecticide applications against aedes aegypti (diptera: culicidae) in mexico.a cluster-randomized controlled trial quantified the entomological efficacy of aerial ultra-low volume (aulv) applications of the insecticide chlorpyrifos against aedes aegypti in puerto vallarta, méxico, during november-october 2017. the trial involved 16 large (1 × 1 km) clusters distributed between treatment-control arms. primary endpoint was the abundance of ae. aegypti indoors (total adults, females, and blood-fed females) collected using prokopack aspirators. after four consecutive weekly ...201931120502
wolbachia strain walba blocks zika virus transmission in aedes aegypti.transinfections of the maternally transmitted endosymbiont wolbachia pipientis can reduce rna virus replication and prevent transmission by aedes aegypti, and also have the capacity to invade wild-type populations, potentially reaching and maintaining high infection frequencies. levels of virus transmission blocking are positively correlated with wolbachia intracellular density. despite reaching high densities in ae. aegypti, transinfections of walba, a strain native to aedes albopictus, showed ...201931120156
larvicidal activity of hypoestes forskaolii (vahl) r. br root extracts against anopheles gambiae giless.s, aedes aegypti l, and culex quinquefasciatussay.aim: this study aimed to evaluate larvicidal activity of hypoestes forskaolii r. br root extract against 3rd instar anopheles gambiae, aedes aegypti, and culex quinquefasciatus. methods: a protocol developed by the world health organization was adopted, with minor modification using chloroform and methanol extracts with concentrations ranging from 25-750 µg/ml. results: the h. forskaolii chloroform extract exhibited very high larvicidal activity after 72 hours of exposure, with lc50 2.0322, 3.89 ...201931118834
characterization of a broad-based mosquito yeast interfering rna larvicide with a conserved target site in mosquito semaphorin-1a genes.rna interference (rnai), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. saccharomyces cerevisiae (baker's yeast) may represent an effective interfering rna expression system that could be used directly for delivery of rna pesticides to mosquito larvae. here we describe characterization of a yeast larvicide developed through bioengineering ...201931118082
[experiences, barriers and facilitators to the implementation of interventions for controlling aedes aegypti in latin america and the caribbean: a qualitative study].this study sought to describe experiences, barriers and facilitators to the implementation of interventions for controlling aedes aegypti in latin america and the caribbean. a qualitative approach with semi-structured in-depth interviews with program implementation experts (19 participants in nine countries) was carried out. we used a software to codify the data and created matrices to compare them. based on our findings, we developed a graphic representation of the theoretical dimensions that e ...201931116251
aedes mosquito infestation in socioeconomically contrasting neighborhoods of panama city.the global expansion and proliferation of aedes aegypti and aedes albopictus represents a growing public health threat due to their capacity to transmit a variety of arboviruses to humans, including dengue, chikungunya, and zika. particularly important in urban regions, where these species have evolved to breed in man-made containers and feed nearly exclusively on human hosts, the threat of vector-borne disease has risen in recent decades due to the growth of cities, progression of climate chang ...201931114946
estimating absolute indoor density of aedes aegypti using removal sampling.quantification of adult aedes aegypti abundance indoors has relied on estimates of relative density (e.g. number of adults per unit of sampling or time), most commonly using traps or timed collections using aspirators. the lack of estimates of the sensitivity of collections and lack of a numerical association between relative and the absolute density of adult ae. aegypti represent a significant gap in vector surveillance. here, we describe the use of sequential removal sampling to estimate absol ...201931113454
use of mosquito repellents to protect against zika virus infection among pregnant women in evaluate the use of repellents among pregnant women as a protective measure against infection with the zika virus.201931112836
predicting aquatic development and mortality rates of aedes aegypti.mosquito-borne pathogens continue to be a significant burden within human populations, with aedes aegypti continuing to spread dengue, chikungunya, and zika virus throughout the world. using data from a previously conducted study, a linear regression model was constructed to predict the aquatic development rates based on the average temperature, temperature fluctuation range, and larval density. additional experiments were conducted with different parameters of average temperature and larval den ...201931112566
the ion channel ppk301 controls freshwater egg-laying in the mosquito aedes aegypti.female aedes aegypti mosquitoes are deadly vectors of arboviral pathogens and breed in containers of freshwater associated with human habitation. because high salinity is lethal to offspring, correctly evaluating water purity is a crucial parenting decision. we found that the deg/enac channel ppk301 and sensory neurons expressing ppk301 control egg-laying initiation and choice in ae. aegypti. using calcium imaging, we found that ppk301-expressing cells show ppk301-dependent responses to water bu ...201931112133
juvenile hormone signaling in insect oogenesis.juvenile hormone (jh) plays a crucial role in insect reproduction, but its molecular mode of action only became clear within the last decade. we here review recent findings revealing the intricate crosstalk between jh and ecdysone signaling with nutrient sensing pathways in drosophila melanogaster, aedes aegypti, tribolium castaneum and locusta migratoria. the finding for a critical role of ecdysis triggering hormone (eth) in both molting and ooogenesis now also highlights the importance of an i ...201931109672
transmission potential of african, asian and american zika virus strains by aedes aegypti and culex quinquefasciatus from guadeloupe (french west indies).zika virus (zikv) is an arbovirus that has dramatically spread in south america and the caribbean regions since 2015. the majority of vector incrimination studies available for zikv showed that aedes aegypti mosquitoes are important vectors for this virus. however, several reports suggest that culex quinquefasciatus mosquitoes may be implicated in zikv transmission in certain urban settings. in the present study, we evaluated the vector competence for zikv of cx. quinquefasciatus and ae. aegypti ...201931109248
pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection.wolbachia's ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. wolbachia-infected aedes aegypti are currently being released in locations such as brazil, which regularly experience concurrent outbreaks of different arboviruses. a. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment.201931107912
tire shops in miami-dade county, florida are important producers of vector mosquitoes.human mobility in urban environments is a central part of urbanization and has determined the layout of how cities are projected, built and renovated. one of the most problematic issues of urbanization is how to properly dispose of used tires, considering the worldwide annual production of approximately 1.4 billion units every year. despite the efforts to properly dispose of used tires, they still represent a major problem for public health, notably serving as potential breeding sites for vector ...201931107881
characterization of a yeast interfering rna larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquito control strategies are vitally needed to address established and emerging arthropod-borne infectious diseases. here we describe the characterization of a yeast interfering rna larvicide that was developed through the genetic engineering of saccharomyces cerevisiae (baker's yeast) to express a short hairpin rna targeting the aedes aegypti synaptotagmin (aae syt) gene. the larvicide effectively silences the aae syt gene, causes defects at the larval neural synapse, and induces high ra ...201931107878
neurological manifestations of pediatric arboviral infections in the americas.dengue, zika, chikungunya and yellow fever viruses are arboviruses transmitted by the mosquito aedes aegypti. these viruses exhibit marked neurotropism but have rarely been studied. here, we conduct an integrative review of the neurological manifestations caused by these arboviruses in the pediatric population. data on patients under 18 years of age were extracted from literature databases. the most frequently reported neurological manifestations were encephalitis, meningitis, seizures, hypotoni ...201931103802
induction of rna interference to block zika virus replication and transmission in the mosquito aedes aegypti.the yellow fever mosquito, aedes aegypti, serves as the primary vector for epidemic transmission of yellow fever, dengue, zika (zikv), and chikungunya viruses to humans. control of ae. aegypti is currently limited to insecticide applications and larval habitat management; however, to combat growing challenges with insecticide resistance, novel genetic approaches for vector population reduction or transmission interruption are being aggressively pursued. the objectives of this study were to asses ...201931103782
plant essential oils synergize various pyrethroid insecticides and antagonize malathion in aedes aegypti.pyrethroid resistance is a significant threat to agricultural, urban and public health pest control activities. because economic incentives for the production of novel active ingredients for the control of public health pests are lacking, this field is particularly affected by the potential failure of pyrethroid-based insecticides brought about by increasing pyrethroid resistance. as a result, innovative approaches are desperately needed to overcome insecticide resistance, particularly in mosqui ...201931102301
prevalence and distribution of dengue virus in aedes aegypti in yogyakarta city before deployment of wolbachia infected aedes aegypti.indonesia is one of the countries where dengue infection is prevalent. in this study we measure the prevalence and distribution of dengue virus (denv) denv-infected aedes aegypti in yogyakarta city, indonesia, during the wet season when high dengue transmission period occurred, as baseline data before implementation of a wolbachia-infected aedes aegypti trial for dengue control. we applied one-step multiplex real time pcr (rt-pcr) for the type-specific-detection of dengue viruses in field-caught ...201931100967
aedes aegypti saliva impairs m1-associated proinflammatory phenotype without promoting or affecting m2 polarization of murine macrophages.during the feeding process, the mouthparts of hematophagous mosquitoes break the skin barrier and probe the host tissue to find the blood. the saliva inoculated in this microenvironment modulates host hemostasis, inflammation and adaptive immune responses. however, the mechanisms involved in these biological activities remain poorly understood and few studies explored the potential roles of mosquito saliva on the individual cellular components of the immune system. here, we report the immunomodu ...201931097013
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