Publications

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acaricide susceptibility in the lone star tick: assay techniques and baseline data. 19817320318
wear and wash persistence of permethrin used as a clothing treatment for personal protection against the lone star tick (acari: ixodidae). 19826123602
immune serum transfer of cutaneous basophil-associated resistance to ticks: mediation by 7sigg1 antibodies.guinea pigs acquired resistance to amblyomma americanum larval ticks after one infestation, resulting in 46% tick rejection when challenged. intravenous transfer of immune serum from twice-infested hosts to naive animals conferred a significant level of immunity resulting in 18 to 30% tick rejection. the minimum effective dose of serum was 3 ml per recipient, and heating the serum at 56 degrees c for 4 hr had no effect on serum activity. fractionation of whole immune serum by gel filtration chro ...19827142697
growth in lone star ticks, amblyomma americanum (acari: ixodidae), feeding on cattle: a laboratory study. 19827120307
antibody- and cell-mediated immune resistance by guinea pigs to adult amblyomma americanum ticks.naive and actively and passively sensitized guinea pigs were infested with adult amblyomma americanum ticks. naive hosts allowed for 75-81% tick yield, 518-581 mg mean tick weight and an average tick feeding period of 12-13 days. actively sensitized and challenged hosts, however, allowed only 47% tick yield (38.9% rejection), replete individuals were significantly reduced in weight (66.1% weight decrease) and required slightly longer time to feed (14 days). passively sensitized hosts were given ...19827149113
a dopamine sensitive adenylate cyclase in the salivary glands of amblyomma americanum (l.).1. enzyme activity, basal or dopamine-stimulated (10 microm), was linear with time to 25 min and with protein concentration to 0.8 mg protein/ml of final assay volume. activity was maximal between ph 7.0 and 7.5. 2. mg2+ maximally stimulated basal or dopamine-sensitive adenylate cyclase activity at about 4 mm. 3. adenylate cyclase had a km of 0.042 mm for atp and maximum velocities for basal and dopamine-stimulated activity of 107 and 179 pmol cyclic amp formed/mg protein per min, respectively. ...19826125338
pressurized sprays of permethrin on clothing for personal protection against the lone star tick (acari: ixodidae). 19827161438
antibodies to spotted fever-group rickettsiae in dogs and prevalence of infected ticks in southern connecticut.blood samples and ticks were obtained from dogs to assess canine exposure to spotted fever-group (sfg) rickettsiae during 1978-1980 in southern connecticut. of the 1,576 dog sera screened by microimmunofluorescence. 174 (11.0%) contained specific antibodies at titers greater than or equal to 1:64 against rickettsia montana (n = 34), r rickettsii (n = 31), r rhipicephali (n = 19), or the unclassified 369-c rickettsia (n = 90). end points greater than or equal to 1:8,192 to r rickettsii and to r r ...19826803626
protein changes in the salivary glands of the female lone star tick, amblyomma americanum, during feeding. 19827077436
blood eosinophil and basophil responses in guinea pigs parasitized by amblyomma americanum ticks.guinea pigs sensitized to tick feeding by a previous infestation express immune resistance when challenged. this state is associated with large accumulations of eosinophils, and especially of basophils, at cutaneous sites of tick feeding. the present study shows that primary and secondary tick feeding stimulate blood eosinophilia and basophilia that reflect the infiltration of these cells in the skin. primary feeding of amblyomma americanum larvae resulted in a mean tick yield of 81%, mean tick ...19827081550
systematic efficacy of ivermectin mk-933 against the lone star tick. 19826896882
ablation of immunity to amblyomma americanum by anti-basophil serum: cooperation between basophils and eosinophils in expression of immunity to ectoparasites (ticks) in guinea pigs.basophils infiltrate the skin and other tissues as part of t lymphocyte and/or antibody-mediated immune responses to certain protein antigens, viruses, tumors, and parasites. although basophils may comprise a significant fraction of leukocytes in these reactions, their precise role has been poorly understood. guinea pigs expressing acquired immunity to the tick amblyomma americanum develop basophil- and eosinophil-rich cutaneous inflammatory responses at tick feeding sites, and tick rejection is ...19827086142
regulation of feeding and ovipositional success of amblyomma americanum ticks. 19827128750
comparison of habitat modification, animal control, and standard spraying for control of the lone star tick. 19827142558
alveolar structure of salivary glands of the lone star tick, amblyomma americanum (l.): unfed females.alveoli in the salivary glands of unfed amblyomma americanum (l.) females (postnymphal ticks that had not yet taken a bloodmeal as an adult) were studied. as in other species of female ixodid ticks, the salivary glands consisted of three alveoli, one agranular and two granular. the agranular alveoli were directly attached to the anterior portion of the main salivary duct, consisted of approximately 13 to 14 cells, and were without valves. six peripheral cells had tortuous, plasma membrane infold ...19827200514
strategic treatment of pastured beef cattle with acaricides: effect on populations of the lone star tick (acari: ixodidae). 19836826885
metazoan parasites of the eastern cottontail rabbit in western kentucky.forty-five eastern cottontail rabbits (sylvilagus floridanus allen), collected over a 15 mo period from two areas of western kentucky, were examined for disease conditions, helminth and arthropod parasites. a single lymphosarcoma, two cutaneous papillomas and the following parasites were identified: hasstilesia tricolor, taenia pisiformis, cittotaenia variabilis, raillietina stilesiella, obeliscoides cuniculi, trichuris leporis, longistriata noviberiae, trichostrongylus calcaratus, dermatoxys ve ...19836842731
pressurized sprays of permethrin and deet on clothing for personal protection against the lone star tick and the american dog tick (acari: ixodidae). 19836875081
lone star tick sighted in newport county. 19836578558
pheromone-induced aggregation of ixodid ticks before host contact.the presence of a pre-feeding aggregation pheromone was demonstrated in the species dermacentor variabilis, dermacentor andersoni, dermacentor parumapertus, amblyomma americanum and haemaphysalis leporispalustris by assay within a petri dish. however, amblyomma maculatum and amblyomma cajennense did not aggregate in the sector containing discs of presumed pheromone within the hour period. d. andersoni and a. americanum recognized each other's pheromone and a. americanum recognized that of h. lep ...19836683667
area control of larvae of the lone star tick (acari: ixodidae) with acaricides. 19836186711
immunohistochemical localization of adenosine 3':5'-cyclic monophosphate in female ixodid tick amblyomma americanum (l.) salivary glands.localization of adenosine 3':5'-cyclic monophosphate (cyclic amp) in alveoli of salivary glands of female amblyomma americanum (l.) was accomplished with an indirect immunofluorescent technique. little cyclic amp fluorescence was seen in type i alveoli in glands of unfed females but considerable fluorescence was seen in type i alveoli of glands obtained from females that had fed. the most intense cyclic amp fluorescence was observed in complex granular cells of type ii and iii alveoli in glands ...19836298395
economic thresholds for lone star ticks (acari: ixodidae) in recreational areas based on a relationship between co2 and human subject sampling. 19836408150
characterization of tick antigens inducing host immune resistance. i. immunization of guinea pigs with amblyomma americanum-derived salivary gland extracts and identification of an important salivary gland protein antigen with guinea pig anti-tick antibodies.guinea pigs immunized by subcutaneous injection of an emulsion of incomplete freund's adjuvant (ifa) containing tick salivary gland extract antigens (sga) from partially fed female ticks expressed a significant level of tick rejection when challenged 17 days later. this level of tick rejection was similar to animals actively sensitized by tick feeding and challenged at the same time. sga emulsified with complete freund's adjuvant (cfa) or administered with saline was ineffective. however, ticks ...19846491289
aerial application of diazinon granules for area control of the lone star tick (acari: ixodidae). 19846512070
bovine resistance to amblyomma americanum ticks: an acquired immune response characterized by cutaneous basophil infiltrates.purebred holstein calves acquired resistance to amblyomma americanum adult ticks after 1 infestation and expressed resistance during a challenge infestation 26 days later. tick yields from resistant animals were normal, but mean tick weight and egg-mass weight were reduced significantly (31 and 32%, respectively). cutaneous cellular responses at tick feeding sites in primary (naive) hosts consisted almost exclusively of mononuclear cells, until 24-h post-infestation when granulocytes appeared. a ...19846543047
effect of host age on the expression of acquired resistance to ticks.young guinea-pigs (350 g) expressed significantly greater levels of acquired resistance to challenge by larval amblyomma americanum ticks than older guinea-pigs (520-800 g). this finding suggests that younger guinea-pigs are immunologically more responsive to tick infestation than older mature individuals. therefore, host age is an important variable in evaluating acquired resistance to ticks.19846698176
amblyomma americanum: a potential vector of lyme disease in new jersey.amblyomma americanum is a likely secondary vector of lyme disease in new jersey. ticks of this species were removed from the site of the characteristic skin lesion known as erythema chronicum migrans on two patients with the disease, and the lyme disease spirochete was isolated from nymphs and adults of this species. that a. americanum is a potential vector is supported by its similarities to ixodes dammini, the known tick vector, in seasonal distribution and host utilization. the extensive rang ...19846710158
ixodid ticks on feral swine in florida.more than 99% of the 645 feral swine (sus scrofa l.) in southern florida harbored ixodid ticks. dermacentor variabilis (american dog tick) was present on 99.6% of the swine and comprised 82.5% of the ticks collected. amblyomma maculatum (gulf coast tick) occurred on 85.9% of the hosts and 17.4% of the collections were of this species. amblyomma americanum (lone star tick) and ixodes scapularis (black-legged tick) were found infrequently and together constituted less than 0.1% of the ticks. pigs ...19846737605
ultralow-volume mists of chlorpyrifos from a tractor-mounted blower for area control of the lone star tick (acari: ixodidae). 19846210316
adenosine-3',5'-monophosphate in salivary glands of unfed and feeding female lone star ticks, amblyomma americanum (l.).basal levels of camp in salivary glands of female lone star ticks were found to be about 5 pmoles/mg protein during all stages of feeding. glands stimulated with 10(-5)m dopamine and 10(-5)m dopamine plus theophylline exhibited significant increases in camp/mg protein. after stimulation by 10(-5)m dopamine was removed, camp decreased faster in glands from slowly feeding ticks (less than 200 mg) than in glands of rapidly feeding ticks (greater than 200 mg).19846149877
ticks on white-tailed deer fawns from southern illinois.seventy-six white-tailed deer (odocoileus virginianus (zimmermann] fawns captured on crab orchard national wildlife refuge in southern illinois during june-july 1980-1983 were aged and examined for ticks. the prevalence of ticks (amblyomma americanum (linnaeus, 1758) and dermacentor variabilis (say, 1821] was 80%; intensity of infestation ranged from one to 123 ticks per fawn, averaging 21.6. tick intensity correlated poorly with age suggesting that there were other, more important determinants ...19846530716
effect of age, application date, and life stage on the susceptibility of free-living lone star ticks (acari: ixodidae) to acaricides. 19846210315
injury thresholds and production loss functions for the lone star tick, amblyomma americanum (acari: ixodidae), on pastured, preweaner beef cattle, bos taurus. 19854056195
weight changes in individual feeding amblyomma americanum female ticks. 19854093818
field method for evaluating the effectiveness of acaricides against lone star ticks (acari: ixodidae) on domestic dogs. 19853973208
apparatus for artificial infestation of pastured beef cattle with the lone star tick (acari: ixodidae). 19853998270
surveillance of lyme disease in the united states, 1982.lyme disease is a tick-borne illness that has been reported from three regions in the united states--the northeast, midwest, and west--which correspond to the distribution of the recognized vectors of the disease, ixodes dammini and ixodes pacificus. in 1982, a surveillance system designed to define the morbidity and geographic distribution for lyme disease by using a clinical case definition received information on 491 definite cases and 38 probable cases. of the definite cases, 489 were acquir ...19853998509
acquisition and expression of resistance by bos indicus and bos indicus x bos taurus calves to amblyomma americanum infestation.purebred and crossbred bos indicus calves were infested 1, 2, or 3 times with 10 female and 5 male amblyomma americanum. resistance was acquired by both the purebred and the crossbred calves after 1 infestation and resulted in statistically significant decreases in the percentages of females that engorged, the mean weights of engorged females, and the mean weights of egg masses. comparisons between breeds of the percent of female ticks that engorged during the first and second infestations indic ...19853998956
evaluation of five popular methods for tick removal.five methods commonly advocated for tick removal from a human or domestic animal were evaluated. the application of petroleum jelly, fingernail polish, 70% isopropyl alcohol, or a hot kitchen match failed to induce detachment of adult american dog ticks (dermacentor variabilis say) attached for either 12 to 15 hours or three to four days. use of forceps or protected fingers enabled satisfactory removal of 29 adult american dog ticks without leaving the mouthparts or attachment cement in the host ...19854000801
human infestation by amblyomma americanum larvae ("seed ticks"). 19854002011
resistance to ixodid tick infestation induced by administration of tick-tissue culture cells.primary tissue culture cells of developing larvae of amblyomma americanum were administered to guinea-pigs never previously exposed to ixodid ticks. guinea-pigs were given 1 x 10(6) primary culture cells on days 0, 7 and 21 by subcutaneous injection and challenged with male and female a. americanum on day 35. a significant degree of induced host tick resistance was expressed by reduced engorgement weight of females, reduced oviposition by those females which did obtain a blood meal, and by death ...19854083961
rickettsia-like organisms in ticks and antibodies to spotted fever-group rickettsiae in mammals from northern mississippi.studies were conducted from april through august during 1974 and 1975 on 30 randomly selected trapping sites in wall doxey state park, holly springs national forest, and 1.6 km south of the park in marshall county, mississippi to provide information on the occurrence of ticks involved in the maintenance and transmission of spotted fever-group rickettsiae in nature. of 365 animals (14 species) collected, 186 (51%) were parasitized by 4,169 ticks. species of ticks collected included: dermacentor v ...19853923212
amblyomma americanum: requirement for host fc receptors in antibody-mediated acquired immune resistance to ticks.guinea pig recipients of anti-tick immune serum or immune peritoneal exudate cells expressed 25 and 30% tick rejection, respectively, when challenged with amblyomma americanum larval ticks. previous studies have shown that igg1 antibodies are responsible for the ability of immune serum to transfer resistance to ticks and to mediate the accompanying, and required, cutaneous basophil response. since igg1 antibodies induce mast cell-mediated passive cutaneous anaphylaxis and cutaneous basophil resp ...19853972060
systemic activity of closantel for control of lone star ticks, amblyomma americanum (l.), on cattle.cattle were treated once at 5 mg/kg orally or subcutaneously or daily at 0.1-5 mg/kg orally or 0.1-1 mg/kg subcutaneously with closantel, n-[5-chloro-4-[(4-chlorophenyl)cyanomethyl]-2-methylphenyl]-2-hydroxy-3, 5-diiodobenzamide, and numbers and weights of engorged females, weights of egg masses and hatch of eggs of lone star ticks, amblyomma americanum, were recorded. effectiveness of treatments on reproduction was determined by comparing total estimated larvae (el) (el = wt. egg mass x est. % ...19853870959
density estimators for populations of the lone star tick, amblyomma americanum (acari: ixodidae), on pastured beef cattle. 19854009621
rejection of ticks from guinea pigs by anti-hapten-antibody-mediated degranulation of basophils at cutaneous basophil hypersensitivity sites: role of mediators other than histamine.previous studies have established that recruitment of basophils to sites of tick feeding in guinea pigs is required to effect immune resistance. in the current study, actively sensitized guinea pigs treated three times daily with h-1 (mepyramine) and h-2 (cimetidine) histamine receptor antagonists, during the challenge tick infestation period, expressed normal resistance to amblyomma americanum larvae. similarly, naive guinea pigs treated with anti-histamines four times daily, beginning 7 days b ...19852578152
spirochetes in ticks and antibodies to borrelia burgdorferi in white-tailed deer from connecticut, new york state, and north carolina.ticks were screened for spirochetes and serum samples from white-tailed deer (odocoileus virginianus) were assayed for antibodies to borrelia burgdorferi during 1983-1984. using fluorescein isothiocyanate-labeled rabbit antibodies produced to b. burgdorferi, the etiologic agent of lyme disease, spirochetes were detected in ixodes dammini (10.5% of 1,193) and dermacentor albipictus (0.6% of 157) adults from connecticut, i. dammini nymphs (49.1% of 108) and adults (64.7% of 99) from armonk, new yo ...19863520030
density perturbation in populations of amblyomma americanum (acari: ixodidae) in beef cattle forage areas in response to two regimens of vegetation management. 19863950182
intradermal infestation of a red fox (vulpes vulpes) by the lone star tick (amblyomma americanum). 19863951048
physiological and biochemical changes in unfed lone star ticks, amblyomma americanum (acari: ixodidae), with increasing age. 19863735326
electrophoretically detectable protein variation in natural populations of the lone star tick, amblyomma americanum (acari: ixodidae).nine populations of amblyomma americanum (l.) were examined electrophoretically for variation of 21 enzymes. only three enzymes were not polymorphic and the average heterozygosity per individual (h) for the species was 0.085 with a range of 0.077 to 0.110, comparing well with values in other arthropods. the average nei identity value for pairwise comparisons among the nine populations was high, 0.994 +/- 0.004 (i +/- sd). these high identity values and the absence of geographic structuring of th ...19863744877
the effect of avermectins on feeding, salivary fluid secretion, and fecundity in some ixodid ticks.we tested the effects of the potent acaricides, avermectin b1a (avm) and 22,23-dihydroavermectin b1 (ivermectin; ivm) when injected directly into partially fed and fully engorged female ticks. when injected into small ticks (amblyomma hebraeum koch), neither drug (up to 100 micrograms/kg b.w.) inhibited subsequent engorgement nor affected oviposition latency, weight of total egg mass laid nor viability of laid eggs. at higher concentrations (1000 and 5000 micrograms/kg b.w.), avm and ivm were ma ...19863451859
comparison of rates of infection by the lyme disease spirochete in selected populations of ixodes dammini and amblyomma americanum (acari: ixodidae).at a major endemic focus in new jersey, 50% of 290 adult ixodes dammini collected in the fall of 1984 were infected with the lyme disease spirochete (borrelia burgdorferi), which was statistically higher than the rate found in the 202 adult ticks (39.6%) examined during the spring. neither sex nor site of collection within the focus significantly affected the infection rate. the observed infection rates were similar to those reported in endemic areas of new york and connecticut. borrelia burgdor ...19863577494
amblyomma americanum: physiochemical isolation of a protein derived from the tick salivary gland that is capable of inducing immune resistance in guinea pigs.crude salivary gland derived proteins from amblyomma americanum ticks were analyzed by physiochemical (gel filtration and ion exchange chromatography) and immunochemical guinea pig igg1 (anti-tick immunoaffinity column) techniques for the presence of antigens responsible for the induction of host immune resistance responses. gel filtration (g-75 sephadex) and ion exchange (diethyl aminoethyl cellulose) chromatography of crude salivary gland antigen yielded multiple fractions, but only one fracti ...19863720900
production system-based model for defining economic thresholds in preweaner beef cattle, bos taurus, infested with the lone star tick, amblyomma americanum (acari: ixodidae). 19863950186
influence of deer exclusion on populations of lone star ticks and american dog ticks (acari: ixodidae). 19863722591
serum basophil-stimulating activity in the guinea-pig during induction of basophilic responses to ovalbumin and tick feeding.we have described functional and biochemical characteristics of a distinct t-cell dependent guinea-pig basophil-stimulating factor (bsf), measured using a sensitive 7-day bone marrow culture assay, standardized with high-activity bsf present in serum-free splenic cell-conditioned medium (cm). in the present studies, the in vivo relevance of bsf was explored during protocols of induction of peripheral blood or tissue basophil responses to ovalbumin (oa) injection or amblyomma americanum tick feed ...19863733145
spotted fever group rickettsiae in the lone star tick, amblyomma americanum (acari: ixodidae). 19863772952
survival of the lone star tick (acari: ixodidae) under flooding conditions: a laboratory evaluation. 19863805482
the development of amblyomma americanum and dermacentor variabilis (acari: ixodidae) fed on rabbits immunized with tick hemolymph.the possibility of interfering with the normal function of tick hemolymph using antihemolymph antibodies taken in with the bloodmeal, was investigated. cell free hemolymph from replete amblyomma americanum and dermacentor variabilis ticks was used to immunize rabbits. immunized rabbits developed high antihemolymph antibody titers (ca. 10(5)) and had no ill side effects. rabbits were simultaneously infested with larvae, nymphs, and adult ticks. the biological performance of ticks fed on immunized ...19873588587
computer simulation of area-wide management strategies for the lone star tick, amblyomma americanum (acari: ixodidae). 19873669024
effects of season and area on ectoparasites of white-tailed deer (odocoileus virginianus) in mississippi.nine species of ectoparasites (4 acari, 2 mallophaga, 1 anoplura, 1 diptera, and 1 siphonaptera) were recovered from 126 white-tailed deer collected in northern, central, and southern mississippi. intensity and prevalence of adults of ixodes scapularis and larvae, nymphs, and adults of amblyomma americanum varied significantly over collection periods, but not between host sexes. lipoptena mazamae occurred on deer from only one study area. although individual deer were heavily parasitized by tric ...19873586204
estimation and economic implications of lone star tick (acari: ixodidae) infestation on weight gain of cattle, bos taurus and bos taurus x bos indicus. 19873584640
effects of gamma radiation on spermatogenesis and fertility of male amblyomma americanum (acari: ixodidae).amblyomma americanum males were treated with 0.5, 1, 2, 3, 4, 8, and 16 krad of gamma radiation. testes of ticks treated with 2, 3, 4, 8, and 16 krad were smaller than those of ticks irradiated at lower levels and controls. no recognizable alteration in timing of spermatogenesis was noted among the different radiation groups, but severe breakdown and depletion of germinal cells was noted at 4, 8, and 16 krad. percent hatch of larvae from crosses of irradiated males and untreated females decrease ...19873585625
computer simulation of population dynamics of the lone star tick, amblyomma americanum (acari: ixodidae). 19873585931
role of small mammals in population dynamics and dissemination of amblyomma americanum and dermacentor variabilis (acari: ixodidae) at land between the lakes, tennessee. 19873295243
a seed tick infestation of the conjunctiva: amblyomma americanum larva.ticks commonly infest humans but rarely bite the eyes. they are vectors of disease; therefore, rapid and complete removal is important. their attachment can be so secure that removal is difficult or hazardous. we excised a larval form of amblyomma americanum from a woodsman's conjunctiva.19873566026
failure to detect infection in fallow deer (cervus dama) exposed to theileria cervi from white-tailed deer.a frozen stabilate was produced from theileria cervi sporozoites in salivary glands of adult amblyomma americanum. the stabilate was inoculated into three fallow deer (cervus dama) and two white-tailed deer (odocoileus virginianus). following inoculation, the white-tailed deer developed parasitemias as determined by blood smear examination at 11 and 13 days postexposure. repeat examination of blood from the three fallow deer for 30 days postexposure failed to reveal observable piro-plasms. these ...19873119873
the ultrastructure of sporogony in theileria cervi (bettencourt et al., 1907) in salivary glands of female amblyomma americanum (l.) ticks.the process of sporogony of theileria cervi in salivary glands of unfed and feeding amblyomma americanum females that were infected as nymphs was studied by light and electron microscopy (sem and tem). sporogony was observed only in granular acini type iii and in cells classified as complex granular. infected acini in salivary glands of both unfed and feeding ticks were enlarged. some stages of parasite development were evident with light microscopy and sem, but the specific events of sporogony ...19873125313
ability to ixodes scapularis, dermacentor variabilis, and amblyomma americanum (acari: ixodidae) to acquire, maintain, and transmit lyme disease spirochetes (borrelia burgdorferi). 19883193425
susceptibility of amblyomma americanum to natural and experimental infections with theileria cervi.one hundred fifty amblyomma americanum were examined between march and september 1986 from cookson hills wildlife refuge in eastern oklahoma (usa). of these ticks, 11% (17 of 150) were infected with theileria cervi. field-collected nymphal ticks had an 8% (3 of 37) prevalence of infection averaging 1.0 infected acini/nymph. female ticks had a 16% prevalence of infection averaging 1.6 infected acini/female; t. cervi was not observed in salivary glands of field-collected male ticks. when laborator ...19883193564
ticks parasitizing humans in a lyme disease endemic area of southern new york state.a total of 126 tick specimens were submitted by tick bite victims to the westchester county department of health, white plains, new york, and to the new york medical college, medical entomology laboratory, armonk, new york, during 1985. these included 96 (76.2%) ixodes dammini, 26 (20.6%) dermacentor variabilis, and two (1.6%) amblyomma americanum. i. dammini parasitism was reported during all months of the year except december and february and involved all life stages. only d. variabilis adults ...19883189288
effects of photoperiod on nymphal diapause in amblyomma americanum (acari: ixodidae). 19883204627
characterization of tick antigens inducing host immune resistance. ii. description of rabbit-acquired immunity to amblyomma americanum ticks and identification of potential tick antigens by western blot analysis.feeding by adult amblyomma americanum ticks induced a level of immunity in rabbits to subsequent tick feeding that resulted in a significant decrease in tick feeding success and fecundity. histological analysis of tick feeding sites in hosts expressing resistance revealed a predominant eosinophil response, with weak basophil and neutrophil infiltrates. while the basophil was never the dominant granulocyte at the tick feeding sites in resistant hosts, this cell exhibited the greatest increase in ...19883291384
evidence for regurgitation by amblyomma americanum.western blot analysis of amblyomma americanum-derived midgut extracts using tick-resistant rabbit and guinea pig antisera demonstrated several unique polypeptides ranging from 7 to 355 kda. these polypeptides were not observed in similar blots of salivary gland extracts or serum from the respective host animals. the data from this study suggest that female a. americanum ticks regurgitate gut material during feeding. such a finding explains the ability of immunization with gut-derived preparation ...19883420794
lone star tick (acari: ixodidae) management by host manipulation through habitat modification. 19883351876
western blot analysis of amblyomma americanum-derived stage-specific and shared antigens using serum from guinea pigs expressing resistance.serum from guinea pigs expressing resistance to larval, nymphal and adult amblyomma americanum ticks was used in western blot analyses to identify potential antigens from egg, larval and nymphal, and female salivary gland extract preparations. the results demonstrate multiple antigens unique to each life stage, as well as several shared proteins between the three life stages. however, it appears as if two particular proteins of 25 and 38 kda may be more important than others, based upon their pr ...19882455376
seasonal changes in feeding on cattle and reproduction by amblyomma americanum (acari: ixodidae) under field conditions. 19883357171
medium-sized mammal hosts of amblyomma americanum and dermacentor variabilis (acari: ixodidae) at land between the lakes, tennessee, and effects of integrated tick management on host infestations. 19883204625
abundance, attachment sites, and density estimators of lone star ticks (acari: ixodidae) infesting white-tailed deer. 19883404547
effect of age on cholinesterase activity and protein of unfed larval ticks.the cholinesterase (ache) activity and total protein in homogenates of unfed larvae of amblyomma americanum (l.), a. cajennense (fabricius), a. maculatum koch, anocentor nitens neumann, and boophilus microplus (canestrini) were determined weekly in ticks 1-6 wk of age. there was a considerable variation in total protein, ache, and the ratio of ache to protein as the ticks aged. however, ache activities were constant or increased with age and total protein levels were constant or decreased. no di ...19892918444
female mediation of feeding time on cattle in adult lone star ticks (acari: ixodidae).the extended feeding time response on cattle of adult lone star ticks, amblyomma americanum (l.), during november through december is facultative and is mediated by the female. mean feeding time (11.8 d) in female ticks taken from the field and held at 14:10 (l:d) photoperiod and 25 degrees c for greater than or equal to 15 d was significantly shorter (p = 0.05) than for females taken directly from the field and fed on cattle (14.6 d). mean feeding times of females from the field paired to males ...19892769719
characterization of ixodid tick salivary-gland gene products, using recombinant dna technology.ticks secrete an array of lesion-maintenance factors into the host via the salivary glands while feeding, some of which elicit an immune response by the host that adversely affects the ability of the tick to feed and reproduce. our approach to characterizing these factors has been to make expression libraries from mrna of salivary glands (from unfed and 3-day-feeding amblyomma americanum females) which will serve as sources of the genes (clones) that code for them. thus far, we have detected 10 ...19892758938
control of lone star ticks (acari: ixodidae) on spanish goats and white-tailed deer with orally administered ivermectin.ivermectin administered orally to spanish goats, capra hircus (l.), or to white-tailed deer, odocoileus virginianus (zimmerman), was highly effective against lone star ticks, amblyomma americanum (l.). for spanish goats, daily oral doses of 20 micrograms/kg resulted in greater than or equal to 2 ppb ivermectin in the blood. this level was sufficient to cause greater than 95% reduction of estimated larvae from feeding ticks. a bioassay with horn flies, haematobia irritans (l.), was developed to e ...19892607030
sites of attachment and density assessment in amblyomma americanum (acari: ixodidae) on nursing beef calves.primary attachment sites of male, female, and nymphal amblyomma americanum (l.) on nursing beef calves were the axillary, inguinal and perianal areas of the body. about 50% of all larvae fed on the ears. a density assessment method which uses the number of a. americanum on less than or equal to 5 body areas to predict the total number of ticks (tick burden) on the left side of a calf is presented. estimates of tick burden for male, female, nymphal and larval a. americanum each require examinatio ...19892714123
habitat use by cattle affects host contact with lone star ticks (acari: ixodidae).the location of zebu, british, and zebu x british cattle in tick-infested plots from march through september 1986 was compared with the location of amblyomma americanum l. in the same plots. in april, may, and june, zebu cattle spent less time (9.3-19.4%) in tick-infested quadrats than british (44.4-63.9%) or zebu-british (49.0-69.3%) cattle. in the same months, the probability that a. americanum would contact zebu cattle (1.5-19.6%) was significantly lower than for british (34.7-41.7%) or zebu- ...19892754101
polyfluoro 1,3-diketones as systemic insecticides.a series of aryl polyfluoro 1,3-diketones were examined for systemic ectoparasiticidal activity in cattle. the compounds demonstrated efficacy against several economically important species of insects and acarina. at dosages of 5 mg/kg x1 or 0.35 mg/kg per day intraruminally, activity was observed against blowfly larvae (phormia regina), adult stable fly (stomoxys calcitrans), and lone star tick (amblyomma americanum). in vivo activity was not directly related to in vitro activity, showing a str ...19892769686
reduction of the amblyomma americanum (acari: ixodidae) population at fort a.p. hill, virginia, by aerial application of diazinon granules: correlation of percentage control with received dose.habitat (280 ha) of lone star tick, amblyomma americanum (l.), was aerially treated with 14% diazinon granules. concentration of acaricide reaching the forest floor ranged from 0.02 to 1.25 kg (ai)/ha. greater than 85% control was achieved at study plots receiving the highest dose. a positive correlation was shown between dose received at the forest floor and percentage of control of adults (r = 0.91) and nymphs (r = 0.86).19892795622
comparison of flagging, walking, trapping, and collecting from hosts as sampling methods for northern deer ticks, ixodes dammini, and lone-star ticks, amblyomma americanum (acari:ixodidae).ticks were sampled by flagging, collecting from the investigator's clothing (walking samples), trapping with dry-ice bait, and collecting from mammal hosts on fire island, ny, u.s.a. the habitat distribution of adult deer ticks, ixodes dammini, was the same in simultaneous collections from the investigator's clothing and from muslin flags. walking and flagging samples can both be biased by differences between investigators, so the same person should do comparative samples whenever possible. walk ...19892806016
experimental infection of six species of ixodid ticks with dugbe virus (family bunyaviridae, genus nairovirus).the vector potential of each of 6 species of colonized north american and african ixodid ticks was assessed by intracoelomic inoculation with dugbe virus (ibar 1792, 14th passage in suckling mouse brain) and viral titers were monitored after selected incubation periods. persistence of dugbe virus for greater than or equal to 53 days in 5 species (dermacentor andersoni, d. variabilis, amblyomma americanum, rhipicephalus appendiculatus, and r. sanguineus) indicates that infection occurred. viral t ...19892496609
[the taxonomic aspects of the transmission of the causative agent of lyme disease].according to world literature data 17 species of ixodid ticks have been studied for natural infection with the lyme disease agent. analysis of the data on the level of the infection, transovarial and transphase transmission has shown that main biological vectors of borrelia burgdorferi are the species of the subgenus ixodes s. str. - i. ricinus, i. persulcatus (eurasia), i. dammini, i. pacificus (north america). potential vectors are i. scapularis, i. dentatus, amblyomma americanum, dermacentor ...19902259524
comparative efficiency of flagging and carbon dioxide-baited sticky traps for collecting the lone star tick, amblyomma americanum (acarina: ixodidae).collections of amblyomma americanum (l.) from five habitat types showed that significantly more ticks of all life stages could be collected by co2-baited sticky traps than by the standard flagging technique. the effect of habitat type and season significantly influenced the number of ticks collected by both techniques. traps baited with carbon dioxide revealed adult tick activity in several habitats several weeks earlier than did flagging, because flagging failed to efficiently recover ticks unl ...19902231613
ectoparasitic and phoretic arthropods of virginia opossums (didelphis virginiana) in central tennessee.thirteen species of ectoparasitic (12) or phoretic (1) arthropods were collected from 26 adult virginia opossums, didelphis virginiana, live-trapped from april through september 1987 in davidson county, tennessee. the cat flea ctenocephalides felis and the american dog tick dermacentor variabilis were the predominant species with respect to mean intensity and prevalence. the macronyssid mite ornithonyssus wernecki and the atopomelid mite didelphilichus serrifer, both specific parasites of this h ...19902380870
intrinsic competence of three ixodid ticks (acari) as vectors of the lyme disease spirochete.we compared the intrinsic vector competence of ixodes dammini spielman et al., dermacentor variabilis (say), and amblyomma americanum (l.) for the lyme disease spirochete (borrelia burgdorferi johnson et al.) on prudence island, rhode island, a lyme disease-endemic site where all three ticks occur together. natural and experimental spirochete infection rates were determined in those ticks and their degree of contact with white-footed mice (peromyscus leucopus), the principal reservoir host, was ...19902388239
population growth rates for amblyomma americanum (acari: ixodidae) on bos indicus, b. taurus and b. indicus x b. taurus cattle.densities of amblyomma americanum (l.) on bos indicus, b. taurus and b. indicus x b. taurus cattle are compared over a 3-year period, and the growth rate (rate of increase or decrease) of parasitic tick populations on each cattle genotype is estimated. average log10 densities of parasitic a. americanum larvae are significantly (p = 0.05) lower on b. indicus cattle than on b. taurus and b. indicus xb. taurus cattle. average log densities of nymphal and adult ticks on b. taurus cattle are signific ...19902261818
amblyomma americanum: identification of tick salivary gland antigens from unfed and early feeding females with comparisons to ixodes dammini and dermacentor variabilis.salivary gland antigens involved in host resistance to tick feeding by amblyomma americanum (lone star tick) have been identified. gland extracts from unfed and partially fed 12-, 48-, 72-, 96-, and 120-hr females and their corresponding midgut tissues were analyzed by immunoblotting with sera from naturally immune and hyperimmune sheep and rabbits. polypeptides at 90, 75, 58, 45, 33, and 23 kda from the salivary glands of a. americanum females were consistently observed with antibodies from bot ...19902298277
comparative field evaluation of permethrin and deet-treated military uniforms for personal protection against ticks (acari).the effectiveness of permethrin-impregnated (pi) versus permethrin-sprayed (ps) military battle dress uniforms was evaluated in protecting soldiers from tick bite. at the same time, an extended-duration formulation of n,n-diethyl-m-toluamide (deet), when applied to clothing only, was evaluated and compared with the permethrin-treated uniforms. testing consisted of exposing subjects, clothed in either untreated or the variously treated uniforms, to field populations of ticks during a series of 30 ...19902231620
geographic occurrence of ixodes scapularis and amblyomma americanum (acari: ixodidae) infesting white-tailed deer in north carolina.a state-wide survey to determine the occurrence and comparative numbers of ticks infecting white-tailed deer (odocoileus virginianus) was conducted in north carolina (usa). one thousand six hundred twenty nine deer were examined in 60 of 100 counties; with the exception of one county in the piedmont region, all tick-infested deer occurred in the coastal plain. ixodes scapularis (46%) and amblyomma americanum (53%) were the most prevalent species encountered and accounted for more than 98% of the ...19902250335
cattle breed alters reproduction in amblyomma americanum (acari: ixodidae).female amblyomma americanum [l.] that feed on bos taurus cattle weigh significantly (p = 0.05) more (mean = 505.4 mg) than females that feed on b. indicus x b. taurus cattle (mean = 450.7 mg) but not females that feed on b. indicus cattle (mean = 489.7 mg). females that feed on b. taurus cattle lay more eggs (mean = 3492) and produce more larvae (mean = 3243) than females that feed on b. indicus cattle (mean = 3103 eggs; 2925 larvae) or b. indicus x b. taurus cattle (mean = 2961 eggs; 2759 larva ...19902081445
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