| three-dimensional ultrastructure of feeding tubes and interconnected endoplasmic reticulum in root-knot nematode-induced giant cells in rose balsam. | we investigated the three-dimensional ultrastructure of feeding tubes and the surrounding region in giant cells induced in rose balsam (impatiens balsamina l.) roots by the root-knot nematode meloidogyne incognita, using osmium maceration coupled with field emission scanning electron microscopy (fe-sem). in the roots of 35-day-old galled rose balsam plants, adult nematodes induced the formation of giant cells containing feeding tubes and numerous organelles, including tubular endoplasmic reticul ... | 2017 | 28204899 |
| genome-wide analysis of excretory/secretory proteins in root-knot nematode, meloidogyne incognita provides potential targets for parasite control. | the root-knot nematode, meloidogyne incognita causes significant damage to various economically important crops. infection is associated with secretion of effector proteins into host cytoplasm and interference with host innate immunity. to combat this infection, the identification and functional annotations of excretory/secretory (es) proteins serve as a key to produce durable control measures. the identification of es proteins through experimental methods are expensive and time consuming while ... | 2017 | 28187376 |
| sequential above- and belowground herbivory modifies plant responses depending on herbivore identity. | herbivore-induced changes in plant traits can cause indirect interactions between spatially and/or temporally separated herbivores that share the same host plant. feeding modes of the herbivores is one of the major factors that influence the outcome of such interactions. here, we tested whether the effects of transient aboveground herbivory for seven days by herbivores of different feeding guilds on tomato plants (solanum lycopersicum) alters their interaction with spatially as well as temporall ... | 2017 | 28178961 |
| phytoalexin phenalenone derivatives inactivate mosquito larvae and root-knot nematode as type-ii photosensitizer. | phytoalexins phenalenones (pns) are phytochemicals biosynthesized inside the plant in responsive to exterior threat. pns are excellent type-ii photosensitizers, which efficiently produce singlet oxygen upon light irradiation. based on the core functional structure of pns, novel pn derivatives were synthesized here and their singlet oxygen generating abilities and their phototoxicity were evaluated. at the presence of light, these pns have photoinduced toxicity towards aedes albopictus larvae and ... | 2017 | 28169356 |
| effects of soil textures on infectivity of root-knot nematodes on carrot. | this study was conducted to examine infectivity (penetration and gall and egg-mass formations) of the root-knot nematodes, meloidogyne incognita and m. hapla, on carrots grown in soil conditions of 5 different soil textures consisting of bed-soil (b) and sand (s) mixtures (b-s mixtures) at the ratios of 10:0, 7:3, 5:5, 3:7, and 0:10. for m. incognita, the nematode penetration rates in b-s of 0:10 (100% sand) were significantly higher than in the other b-s mixtures, more greatly at 2 and 5 days a ... | 2017 | 28167889 |
| microbial modulation of bacoside a biosynthetic pathway and systemic defense mechanism in bacopa monnieri under meloidogyne incognita stress. | plant-associated beneficial microbes have been explored to fulfill the imperative function for plant health. however, their impact on the host secondary metabolite production and nematode disease management remains elusive. our present work has shown that chitinolytic microbes viz., chitiniphilus sp. mtn22 and streptomyces sp. mtn14 singly as well as in combination modulated the biosynthetic pathway of bacoside a and systemic defense mechanism against meloidogyne incognita in bacopa monnieri. in ... | 2017 | 28157221 |
| acetic acid, 2-undecanone, and (e)-2-decenal ultrastructural malformations on meloidogyne incognita. | the use of natural compounds to control phytonematodes is significantly increasing, as most of the old synthetic pesticides have been banned due to their eco-hostile character. plant secondary metabolites are now evaluated as biologically active molecules against meloidogyne spp. but their target site in the nematode body is rarely specified. herein, we report on the ultrastructure modifications of the meloidogyne incognita j2 after treatment with nematicidal plant secondary metabolites, that is ... | 2016 | 28154431 |
| grafting and paladin pic-21 for nematode and weed management in vegetable production. | two years of field trials conducted in a meloidogyne incognita-infested field evaluated grafting and paladin pic-21 (dimethyl disulfide:chloropicrin [dmds:pic] 79:21) for root-knot nematode and weed control in tomato and melon. tomato rootstocks evaluated were; 'tx301', 'multifort', and 'aloha'. 'florida 47' was the scion and the nongrafted control. a double crop of melon was planted into existing beds following tomato harvest. melon rootstocks, c. metulifer and 'tetsukabuto', were evaluated wit ... | 2016 | 28154429 |
| gene expression analysis in musa acuminata during compatible interactions with meloidogyne incognita. | endoparasitic root-knot nematodes (rkns) (meloidogyne spp.) cause considerable losses in banana (musa spp.), with meloidogyne incognita a predominant species in cavendish sub-group bananas. this study investigates the root transcriptome in musa acuminata genotypes 4297-06 (aa) and cavendish grande naine (cav; aaa) during early compatible interactions with m. incognita methods: roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological ... | 2017 | 28130221 |
| single basal application of thiacloprid for the integrated management of meloidogyne incognita and bemisia tabaci in tomato crops. | tomato growers commonly face heavy nematode (meloidogyne incognita) and whitefly (b-biotype bemisia tabaci) infestations, and previous studies demonstrated that thiacloprid could be used to control m. incognita and b. tabaci in cucumber. however, the efficacy of a single basal application of thiacloprid to control both pests and its effect on yield in tomato remains unknown. in this study, the potential of thiacloprid application to the soil for the integrated control of m. incognita and b. taba ... | 2017 | 28120937 |
| exploiting cell cycle inhibitor genes of the krp family to control root-knot nematode induced feeding sites in plants. | cell cycle control in galls provoked by root-knot nematodes involves the activity of inhibitor genes like the arabidopsis ick/krp members. ectopic krp1, krp2 and krp4 expression resulted in decreased gall size by inhibiting mitotic activity, whereas krp6 induces mitosis in galls. herein, we investigate the role of krp3, krp5 and krp7 during gall development and compared their role with previously studied members of this class of cell cycle inhibitors. overexpression of krp3 and krp7 culminated i ... | 2017 | 28103637 |
| nematode root herbivory in tomato increases leaf defenses and reduces leaf miner oviposition and performance. | the outcome of plant-mediated interactions among herbivores from several feeding guilds has been studied intensively. however, our understanding on the effects of nematode root herbivory on leaf miner oviposition behavior and performance remain limited. in this study, we evaluated whether meloidogyne incognita root herbivory affects tuta absoluta oviposition preference on solanum lycopersicum plants and the development of the resulting offspring. to investigate the m. incognita-herbivory induced ... | 2017 | 28084560 |
| comparative efficacy of different approaches to managing meloidogyne incognita on green bean. | a greenhouse study was conducted to compare the relative efficacy of different approaches to managing meloidogyne incognita on green bean. these approaches included chemical (fumigant, non-fumigant, seed dressing, and seed dip), biological (the egg-parasitic fungus, paecilomyces lilacinus and the mycorrhizal fungus glomus sp.), physical (soil solarization), and cultural (chicken litter and urea) methods. accordingly, nine different control materials and application methods plus nematode-infected ... | 2017 | 28053585 |
| nematicidal activity of trans-2-hexenal against southern root-knot nematode (meloidogyne incognita) on tomato plants. | botanical nematicides have recently received increasing interest because of the high risks of some traditional nematicides to human health and the environment. this study evaluated the nematicidal activity of a plant volatile, trans-2-hexenal, against meloidogyne incognita. this compound exhibited higher activity in a fumigation experiment than in the aqueous phase in vitro. both in pot tests and in field trials, trans-2-hexenal showed significant efficacy against m. incognita while maintaining ... | 2017 | 28048941 |
| a whole-plant perspective reveals unexpected impacts of above- and belowground herbivores on plant growth and defense. | trade-offs between plant growth and defense are central to theoretical frameworks used to study the ecology and evolution of plant defense against herbivores. however, these frameworks, as well as the experiments designed to test them, rarely include belowground herbivores. we experimentally challenged seedlings of the tropical shrub solanum lycocarpum (solanaceae) with either aboveground foliar herbivores (spodoptera caterpillars) or belowground root herbivores (the nematode meloidogyne incogni ... | 2017 | 27935027 |
| transcriptome analysis of resistant and susceptible tobacco (nicotiana tabacum) in response to root-knot nematode meloidogyne incognita infection. | the root-knot nematode (rkn) meloidogyne incognita reproduces on the roots of tobacco (nicotiana tabacum), damaging crops, reducing crop yield, and causing economic losses annually. the development of resistant genotypes is an alternative strategy to effectively control these losses. however, the molecular mechanism responsible for host pathogenesis and defense responses in tobacco specifically against rkns remain poorly understood. here, root transcriptome analysis of resistant (yuyan12) and su ... | 2017 | 27914810 |
| resistance-breaking population of meloidogyne incognita utilizes plant peroxidase to scavenge reactive oxygen species, thereby promoting parasitism on tomato carrying mi-1 gene. | resistance conferred by the mi-1 gene from solanum peruvianum is effective and widely used for controlling root-knot nematodes (rkns, meloidogyne spp.). however, breakdown of resistance by rkns seriously threatens the durable application of the resistance resource. here, a resistance-breaking population of m. incognita was selected from an avirulent population by continuously inoculating on mi-1-carrying tomato. histological observations showed the resistance-breaking population would not induce ... | 2017 | 27836538 |
| shifting from priming of salicylic acid- to jasmonic acid-regulated defences by trichoderma protects tomato against the root knot nematode meloidogyne incognita. | beneficial root endophytes such as trichoderma spp. can reduce infections by parasitic nematodes through triggering host defences. little is currently known about the complex hormone signalling underlying the induction of resistance. in this study, we investigated whether trichoderma modulates the hormone signalling network in the host to induce resistance to nematodes. we investigated the role and the timing of the jasmonic acid (ja)- and salicylic acid (sa)-regulated defensive pathways in tric ... | 2017 | 27801946 |
| advancements in breeding, genetics, and genomics for resistance to three nematode species in soybean. | integration of genetic analysis, molecular biology, and genomic approaches drastically enhanced our understanding of genetic control of nematode resistance and provided effective breeding strategies in soybeans. three nematode species, including soybean cyst (scn, heterodera glycine), root-knot (rkn, meloidogyne incognita), and reniform (rn, rotylenchulus reniformis), are the most destructive pests and have spread to soybean growing areas worldwide. host plant resistance has played an important ... | 2016 | 27796432 |
| stage-wise identification and analysis of mirna from root-knot nematode meloidogyne incognita. | in this study, we investigated global changes in mirnas of meloidogyne incognita throughout its life cycle. small rna sequencing resulted in approximately 62, 38, 38, 35, and 39 mb reads in the egg, j2, j3, j4, and female stages, respectively. overall, we identified 2724 known and 383 novel mirnas (read count > 10) from all stages, of which 169 known and 13 novel mirna were common to all the five stages. among the stage-specific mirnas, mir-286 was highly expressed in eggs, mir-2401 in j2, mir-8 ... | 2016 | 27775666 |
| mitochondrial haplotype-based identification of root-knot nematodes (meloidogyne spp.) on cut foliage crops in florida. | florida accounts for more than 75% of the national cut foliage production. unfortunately, root-knot nematodes (rkn) (meloidogyne spp.) are a serious problem on these crops, rendering many farms unproductive. currently, information on the meloidogyne spp. occurring on most commonly cultivated cut foliage crops in florida, and tools for their rapid identification are lacking. the objectives of this study were to (i) identify specific rkn infecting common ornamental cut foliage crops in florida and ... | 2016 | 27765993 |
| the infection of cucumber (cucumis sativus l.) roots by meloidogyne incognita alters the expression of actin-depolymerizing factor (adf) genes, particularly in association with giant cell formation. | cucumber (cucumis sativus l.) is threatened by substantial yield losses due to the south root-knot nematode (meloidogyne incognita). however, understanding of the molecular mechanisms underlying the process of nematode infection is still limited. in this study, we found that m. incognita infection affected the structure of cells in cucumber roots and treatment of the cytoskeleton inhibitor (cytochalasin d) reduced root-knot nematode (rkn) parasitism. it is known that actin-depolymerizing factor ... | 2016 | 27695469 |
| evaluation of in vitro and in vivo nematicidal potential of a multifunctional streptomycete, streptomyces hydrogenans strain dh16 against meloidogyne incognita. | the present work demonstrated the nematicidal potential of streptomyces hydrogenans strain dh16 (a strain with strong antagonism against fungal phytopathogens and insect pest) against meloidogyne incognita. the culture supernatant and solvent extract significantly inhibited egg hatching (almost 100%) along with j2 mortality of more than 95% after 96h. the nematicidal activity of 10-(2,2-dimethyl-cyclohexyl)-6,9-dihydroxy-4,9-dimethyl-dec-2-enoic acid methyl ester (sh2; a new antifungal compound) ... | 2016 | 27664743 |
| direct nematicidal effects of methyl jasmonate and acibenzolar-s-methyl against meloidogyne incognita. | the aim of this study was to examine the nematicidal properties of two defence inducers against the root-knot nematode meloidogyne incognita. a direct-contact bioassay was applied to evaluate the nematicidal effects of acibenzolar-s-methyl (asm) and methyl jasmonate (meja) on second-stage juveniles (j2). nematodes were incubated in different concentrations of these compounds, and the numbers of immobile nematodes were counted after 24 and 48 h post incubation. tap water was then added to verify ... | 2017 | 27658537 |
| expression of a cystatin transgene in eggplant provides resistance to root-knot nematode, meloidogyne incognita. | root-knot nematodes (rkn) cause substantial yield decline in eggplant and sustainable management options to minimize crop damage due to nematodes are still limited. a number of genetic engineering strategies have been developed to disrupt the successful plant-nematode interactions. among them, delivery of proteinase inhibitors from the plant to perturb nematode development and reproduction is arguably the most effective strategy. in the present study, transgenic eggplant expressing a modified ri ... | 2016 | 27516765 |
| a novel meloidogyne incognita effector misp12 suppresses plant defense response at latter stages of nematode parasitism. | secreted effectors in plant root-knot nematodes (rkns, or meloidogyne spp.) play key roles in their parasite processes. currently identified effectors mainly focus on the early stage of the nematode parasitism. there are only a few reports describing effectors that function in the latter stage. in this study, we identified a potential rkn effector gene, misp12, that functioned during the latter stage of parasitism. misp12 was unique in the meloidogyne spp., and highly conserved in meloidogyne in ... | 2016 | 27446188 |
| efficacy of various application methods of fluensulfone for managing root-knot nematodes in vegetables. | fluensulfone is a new nematicide in the flouroalkenyl chemical group. a field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of meloidogyne spp. in cucumber (cucumis sativus). treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (ppi) or via different drip irrigation methods: drip without pulse irrigation (drip np), pulse irrigation 1 hr after treatment (drip +1p), and treatment at ... | 2016 | 27418698 |
| effect of plant resistance and bioact wg (purpureocillium lilacinum strain 251) on meloidogyne incognita in a tomato-cucumber rotation in a greenhouse. | the effectiveness of combining resistant tomato with bioact wg (purpureocillium lilacinum strain 251, pl251) against meloidogyne incognita was assessed in a tomato-cucumber rotation in a greenhouse over 2 years. additionally, the enzymatic activity of the fungus, the percentage of fungal egg and juvenile parasitism, cardinal temperatures and the effect of water potential on mycelial growth and the soil receptivity to pl251 were determined in vitro. | 2017 | 27414251 |
| untargeted metabolomics of tomato plants after root-knot nematode infestation. | after 2 months from the infestation of tomato plants with the root-knot nematode (rkn) meloidogyne incognita, we performed a gas chromatography-mass spectrometry untargeted fingerprint analysis for the identification of characteristic metabolites and biomarkers. principal component analysis, and orthogonal projections to latent structures discriminant analysis suggested dramatic local changes of the plant metabolome. in the case of tomato leaves, β-alanine, phenylalanine, and melibiose were indu ... | 2016 | 27389052 |
| coupling of mic-3 overexpression with the chromosomes 11 and 14 root-knot nematode (rkn) (meloidogyne incognita) resistance qtls provides insights into the regulation of the rkn resistance response in upland cotton (gossypium hirsutum). | genetic analysis of mic-3 transgene with rkn resistance qtls provides insight into the resistance regulatory mechanism and provides a framework for testing additional hypotheses. resistance to root-knot nematode (rkn) (meloidogyne incognita) in upland cotton (gossypium hirsutum) is mediated by two major quantitative trait loci (qtl) located on chromosomes 11 and 14. the mic-3 (meloidogyne induced cotton3) protein accumulates specifically within the immature galls of rkn-resistant plants that pos ... | 2016 | 27314265 |
| synthesis, nematocidal activity and docking study of novel chiral 1-(3-chloropyridin-2-yl)-3-(trifluoromethyl)-1h-pyrazole-4-carboxamide derivatives. | a series of novel chiral fluorinated pyrazole carboxamides derivatives were designed and synthesized. all these title compounds were confirmed by nmr and ms. the primarily nematocidal activity results indicated that some of them exhibited good control efficacy against the tomato root-knot nematode disease caused by meloidogyne incognita. the docking results indicated that compound 5n interact with amino acid residue tyr 121, trp 279 of ache via hydrogen bond. | 2016 | 27301365 |
| influence of root-knot nematode infestation on antioxidant enzymes, chlorophyll content and growth in pogostemon cablin (blanco) benth. | plants adapt themselves to overcome adverse environmental conditions, and this involves a plethora of concurrent cellular activities. physiological experiments or metabolic profiling can quantify this response. among several diseases of pogostemon cablin (blanco) benth. (patchouli), root-knot nematode infection caused by meloidogyne incognita (kofoid and white) chitwood causes severe damage to the plant and hence, the oil production. in the present study, we identified m. incognita morphological ... | 2016 | 27295922 |
| potent nematicidal activity of maleimide derivatives on meloidogyne incognita. | different maleimide derivatives were synthesized and assayed for their in vitro activity on the soil inhabiting, plant-parasitic nematode meloidogyne incognita, also known as root-knot nematode. the compounds maleimide, n-ethylmaleimide, n-isopropylmaleimide, and n-isobutylmaleimide showed the strongest nematicidal activity on the second stage juveniles of the root-knot nematode with ec50/72h values of 2.6 ± 1.3, 5.1 ± 3.4, 16.2 ± 5.4, and 19.0 ± 9.0 mg/l, respectively. we also determined the ne ... | 2016 | 27249054 |
| [efficiency of trichoderma longibrachiatum t6 in the control of meloidogyne incognita and its rhizosphere colonization in cucumber]. | efficiency of different concentrations of trichoderma longibrachiatum t6 against meloidogyne incognita and its rhizosphere colonization in cucumber were determined in greenhouse experiments. the results of rhizosphere colonization experiments showed that the number of colonies in cucumber soil and root increased significantly ten weeks after inoculation with the second stage juveniles of m. incognita and different concentrations of t. longibrachiatum t6, and there was significant difference in d ... | 2016 | 27228616 |
| resistance to southern root-knot nematode (meloidogyne incognita) in wild watermelon (citrullus lanatus var. citroides). | southern root-knot nematode (rkn, meloidogyne incognita) is a serious pest of cultivated watermelon (citrullus lanatus var. lanatus) in southern regions of the united states and no resistance is known to exist in commercial watermelon cultivars. wild watermelon relatives (citrullus lanatus var. citroides) have been shown in greenhouse studies to possess varying degrees of resistance to rkn species. experiments were conducted over 2 yr to assess resistance of southern rkn in c. lanatus var. citro ... | 2016 | 27168648 |
| poplar-root knot nematode interaction: a model for perennial woody species. | plant root-knot nematode (rkn) interaction studies are performed on several host plant models. though rkn interact with trees, no perennial woody model has been explored so far. here, we show that poplar (populus tremula × p. alba) grown in vitro is susceptible to meloidogyne incognita, allowing this nematode to penetrate, to induce feeding sites, and to successfully complete its life cycle. quantitative reverse transcription-polymerase chain reaction analysis was performed to study changes in p ... | 2016 | 27135257 |
| impact of direct and indirect application of rising furfural concentrations on viability, infectivity and reproduction of the root-knot nematode, meloidogyne incognita in pisum sativum. | the gradual withdraw of several broadly used nematicides from market has enhanced the need to develop sustainable and eco-friendly alternatives with nematicidal properties. furfural is one of the promising alternatives to fill this need. baseline information about the impact of furfural on egg hatch, penetration potential and ultrastructure of nematode is lacking. in this study, the reagent-grade (purity ≥ 99.0%) of furfural was applied against meloidogyne incognita. in vitro tests showed gradua ... | 2016 | 27133267 |
| effects of tomato root exudates on meloidogyne incognita. | plant root exudates affect root-knot nematodes egg hatch. chemicals in root exudates can attract nematodes to the roots or result in repellence, motility inhibition or even death. however, until recently little was known about the relationship between tomato root exudates chemicals and root-knot nematodes. in this study, root exudates were extracted from three tomato rootstocks with varying levels of nematode resistance: baliya (highly resistant, hr), rs2 (moderately resistant, mr) and l-402 (hi ... | 2016 | 27128659 |
| early development of the root-knot nematode meloidogyne incognita. | detailed descriptions of the early development of parasitic nematodes are seldom available. the embryonic development of the plant-parasitic nematode meloidogyne incognita was studied, focusing on the early events. | 2016 | 27122249 |
| ozonated water reduces susceptibility in tomato plants to meloidogyne incognita by the modulation of the antioxidant system. | few studies have been carried out on the effect of ozonated water (o3 wat) on the oxidative stress of root systems and, in particular, in combination with biotic stress. the aim of this study was to determine whether aqueous ozone is effective in the control of root-knot nematode (rkn) infection and to investigate the concomitant changes in the basal defence system. a tomato cultivar susceptible to meloidogyne incognita was treated with o3 wat as a soil drench. no negative effects were seen foll ... | 2017 | 27071373 |
| evidence for the involvement of nematocidal toxins of purpureocillium lilacinum 6029 cultured on karanja deoiled cake liquid medium. | in present study, in vitro nematocidal bioassays, ft-ir and hplc analysis were employed to demonstrate the involvement of toxins of purpureocillium lilacinum in killing root-knot nematodes (meloidogyne incognita). during growth study, maximum mycelial biomass (10.52 g/l) in de-oiled karanja cake medium was achieved on 8th day while complete mortality of nematodes was obtained by 6th day filtrate (fksm). maximum production of crude nematocidal toxin was recorded on 7th day suggesting that the tox ... | 2016 | 27038952 |
| sensitivity of meloidogyne incognita and rotylenchulus reniformis to fluopyram. | fluopyram is a succinate dehydrogenase inhibitor (sdhi) fungicide that is being evaluated as a seed treatment and in-furrow spray at planting on row crops for management of fungal diseases and its effect on plant-parasitic nematodes. currently, there are no data on nematode toxicity, nematode recovery, or effects on nematode infection for meloidogyne incognita or rotylenchulus reniformis after exposure to low concentrations of fluopyram. nematode toxicity and recovery experiments were conducted ... | 2015 | 26941460 |
| root-knot nematodes induce pattern-triggered immunity in arabidopsis thaliana roots. | root-knot nematodes (rkns; meloidogyne spp.) are plant parasites with a broad host range causing great losses worldwide. to parasitize their hosts, rkns establish feeding sites in roots known as giant cells. the majority of work studying plant-rkn interactions in susceptible hosts addresses establishment of the giant cells and there is limited information on the early defense responses. here we characterized early defense or pattern-triggered immunity (pti) against rkns in arabidopsis thaliana. ... | 2016 | 26892116 |
| msp40 effector of root-knot nematode manipulates plant immunity to facilitate parasitism. | root-knot nematodes (rkns) are obligate biotrophic parasites that invade plant roots and engage in prolonged and intimate relationships with their hosts. nematode secretions, some of which have immunosuppressing activity, play essential roles in successful parasitism; however, their mechanisms of action remain largely unknown. here, we show that the rkn-specific gene mimsp40, cloned from meloidogyne incognita, is expressed exclusively in subventral oesophageal gland cells and is strongly upregul ... | 2016 | 26797310 |
| mi-flp-18 and mi-mpk-1 genes are potential targets for meloidogyne incognita control. | meloidogyne incognita is a major plant parasite that causes root-knot disease in numerous agricultural crops. this nematode has severely affected greenhouse crops in china. chemical insecticides are generally used to control this pest, but they have adverse environmental and human toxicity effects; hence, safe and effective strategies for controlling the root-knot nematode (rkn) are necessary. fmrfamide-like peptides (flps) have diverse physiological and biological effects on the locomotory, fee ... | 2016 | 26785173 |
| resistance of upland-rice lines to root-knot nematode, meloidogyne incognita. | despite the benefits of crop rotation, occurrence of nematodes is a common problem for almost all crops within the cerrado biome, especially for rice. the use of resistant cultivars is one of the main methods for control of nematodes. thus, the present study aimed to evaluate the reaction of 36 upland-rice lines, with desirable agronomic characteristics, according to their resistance to root-knot nematodes (meloidogyne incognita). the experimental design was entirely randomized with four replica ... | 2015 | 26782379 |
| identification and characterization of micrornas in the plant parasitic root-knot nematode meloidogyne incognita using deep sequencing. | the root-knot nematode meloidogyne incognita is among the most damaging plant-parasitic pests of several crops including cotton (gossypium hirsutum) and tomato (lycopersicon escultentum). recently, a genome has become available for m. incognita, which greatly facilitates investigation of the interactions between m. incognita and its plant hosts at the molecular level and enables formation of hypotheses concerning development at the cellular level. micrornas (mirnas) are a class of small rna mole ... | 2016 | 26743520 |
| development and reproductive potential of tyrophagus putrescentiae (acari: acaridae) on plant-parasitic nematodes and artificial diets. | this study investigated development, reproduction and life table parameters of the astigmatid mold mite tyrophagus putrescentiae (schrank) (acari: acaridae) feeding on egg-masses or adult females of the nematode meloidogyne incognita, egg-masses of the nematode rotylenchulus reniformis, ras cheese or yeast at 25 ± 1 °c, 70 ± 10 % rh in the dark. immature developmental times were shorter when the mite was fed females of m. incognita followed by yeast. different prey/diet types had no significant ... | 2016 | 26692383 |
| [eupolyphaga frass and its extracts protected tomato from meloidogyne incognita infestation]. | the control effects of eupolyphaga (eupolyphaga sinensis walker) frass and its extracts on meloidogyne incognita were studied through laboratory assays and pot experiments, and the organic volatile compounds and oligochitosan contents in the frass were analyzed. the results indicated that the nematode immobility and mortality was significantly increased with increasing the extract concentration and treatment time. compared with the control, egg hatching was significantly inhibited when the extra ... | 2015 | 26685616 |
| in vivo imaging of microtubule organization in dividing giant cell. | mitosis which is a major step during plant development can also be observed in physiopathological conditions. during the compatible interaction between the root-knot nematode meloidogyne incognita and its host arabidopsis, the pathogen induce through repeated divisions without complete cytokinesis the formation of hypertrophied and multinucleate feeding cells, named giant cells. due to the presence of hypertrophied plant cell material surrounding the giant cells, classical live cell imaging gave ... | 2016 | 26659960 |
| metagenomic insights into communities, functions of endophytes, and their associates with infection by root-knot nematode, meloidogyne incognita, in tomato roots. | endophytes are known to play important roles in plant's health and productivity. in this study, we investigated the root microbiome of tomato in association with infection by root knot nematodes. our objectives were to observe the effects and response of the bacterial endophytes before nematode attacks and to reveal the functional attributes of microbes in plant health and nematode pathogenesis. community analysis of root-associated microbiomes in healthy and nematode-infected tomatoes indicated ... | 2015 | 26603211 |
| gotu kola (centella asiatica): nutritional properties and plausible health benefits. | centella asiatica l. (gotu kola) is a nutritionally important plant and a valued traditional medicine in south east asia. in this review, the chemical composition, nutritional values, and health benefits of c. asiatica have been discussed in detail to emphasize its usage as traditional food and medicine. c. asiatica is one of the most commonly used green leafy vegetables (glvs) in some countries including sri lanka due to its high amounts of medicinally important triterpenoids and beneficial car ... | 2015 | 26602573 |
| inheritance and identification of a major quantitative trait locus (qtl) that confers resistance to meloidogyne incognita and a novel qtl for plant height in sweet sorghum. | southern root-knot nematodes (meloidogyne incognita) are a pest on many economically important row crop and vegetable species and management relies on chemicals, plant resistance, and cultural practices such as crop rotation. little is known about the inheritance of resistance to m. incognita or the genomic regions associated with resistance in sorghum (sorghum bicolor). in this study, an f2 population (n = 130) was developed between the resistant sweet sorghum cultivar 'honey drip' and the susc ... | 2015 | 26574655 |
| host transcriptional profiling at early and later stages of the compatible interaction between phaseolus vulgaris and meloidogyne incognita. | the root-knot nematode (meloidogyne incognita) is one of most devastating pathogens that attack the common bean crop. although there is evidence that some cultivars have race-specific resistance against m. incognita, these resistance sources have not proved effective, and nematodes are able to circumvent the host's defense system. we constructed rna-seq based libraries and used a high-throughput sequencing platform to analyze the plant responses to m. incognita. assessments were performed at 4 a ... | 2016 | 26551451 |
| exposure to double-stranded rna mediated by tobacco rattle virus leads to transcription up-regulation of effector gene mi-vap-2 from meloidogyne incognita and promotion of pathogenicity in progeny. | meloidogyne spp. are economically important plant parasites and cause enormous damage to agriculture world-wide. these nematodes use secreted effectors which modify host cells, allowing them to obtain the nutrients required for growth and development. a better understanding of the roles of effectors in nematode parasitism is critical for understanding the mechanisms of nematode-host interactions. in this study, mi-vap-2 of meloidogyne incognita, a gene encoding a venom allergen-like protein, was ... | 2016 | 26545953 |
| in vitro nematicidal activity of aryl hydrazones and comparative gc-ms metabolomics analysis. | a series of aryl hydrazones were synthesized and in vitro assayed for their activity on the root-knot nematode meloidogyne incognita. the phenylhydrazones of thiophene-2-carboxyaldehyde 5, 3-methyl-2-thiophenecarboxyaldehyde, 6, and salicylaldehyde, 2, were the most potent with ec50/48h values of 16.6 ± 2.2, 23.2 ± 2.7, and 24.3 ± 1.4 mg/l, respectively. a gc-ms metabolomics analysis, after in vitro nematode treatment with hydrazone 6 at 100 mg/l for 12 h, revealed elevated levels of fatty acids ... | 2015 | 26528945 |
| effect of emamectin benzoate on root-knot nematodes and tomato yield. | southern root-knot nematode (meloidogyne incognita) is an obligate, sedentary endoparasite of more than 3000 plant species, that causes heavy economic losses and limit the development of protected agriculture of china. as a biological pesticide, emamectin benzoate has effectively prevented lepidopteran pests; however, its efficacy to control m. incognita remains unknown. the purpose of the present study was to test soil application of emamectin benzoate for management of m. incognita in laborato ... | 2015 | 26509680 |
| tracking changes in life-history traits related to unnecessary virulence in a plant-parasitic nematode. | evaluating trade-offs in life-history traits of plant pathogens is essential to understand the evolution and epidemiology of diseases. in particular, virulence costs when the corresponding host resistance gene is lacking play a major role in the adaptive biology of pathogens and contribute to the maintenance of their genetic diversity. here, we investigated whether life-history traits directly linked to the establishment of plant-nematode interactions, that is, ability to locate and move toward ... | 2015 | 26380696 |
| nematicidal triterpenoids from lantana camara. | a new triterpene, lancamarolide (1), and seven known triterpenes, oleanonic acid (2), lantadene a (3), 11α-hydroxy-3-oxours-12-en-28-oic acid (4), betulinic acid (5), lantadene b (6), and lantaninilic acid (7) were isolated from the aerial parts of lantana camara in the course of bioassay-guided isolation, and their nematicidal activities against meloidogyne incognita, the root knot nematode, were carried out. oleanonic acid was found to be the most active compound and exhibited 80% mortality af ... | 2015 | 26363887 |
| management of root-knot nematode (meloidogyne incognita) in bottle gourd using different botanicals in pots. | a pot experiment was conducted to study the efficacy of different botanicals in varying doses for management of root-knot nematode, m. incognita in bottle gourd. the results exhibited that madar (calotropis procera) and neem (azadirachta indica) leaves application proved to be more effective in improving plant growth characters and reducing root-knot index and final nematode population. among the doses tested, higher dose of 1.5 % (w/w) was more effective than lower ones. | 2015 | 26345048 |
| interaction between meloidogyne incognita and rhizoctonia solani on green beans. | the interaction between meloidogyne incognita (race 2) and rhizoctonia solani (ag 4) in a root rot disease complex of green beans (phaseolus vulgaris) was examined in a greenhouse pot experiment. three week-old seedlings (cv. contender) were inoculated with the nematode and/or the fungus in different combinations and sequences. two months after last nematode inoculation, the test was terminated and data were recorded. the synchronized inoculation by both pathogens (n + f) increased the index of ... | 2015 | 26288560 |
| identification of micrornas in meloidogyne incognita using deep sequencing. | micrornas play important regulatory roles in eukaryotic lineages. in this paper, we employed deep sequencing technology to sequence and identify micrornas in m. incognita genome, which is one of the important plant parasitic nematodes. we identified 102 m. incognita microrna genes, which can be grouped into 71 nonredundant mirnas based on mature sequences. among the 71 mirans, 27 are known mirnas and 44 are novel mirnas. we identified seven mirna clusters in m. incognita genome. four of the seve ... | 2015 | 26241472 |
| mi-1-mediated nematode resistance in tomatoes is broken by short-term heat stress but recovers over time. | tomato (solanum lycopersicum l.) is among the most valuable agricultural products, but meloidogyne spp. (root-knot nematode) infestations result in serious crop losses. in tomato, resistance to root-knot nematodes is controlled by the gene mi-1, but heat stress interferes with mi-1-associated resistance. inconsistent results in published field and greenhouse experiments led us to test the effect of short-term midday heat stress on tomato susceptibility to meloidogyne incognita race 1. under cont ... | 2015 | 26170475 |
| yellow pigment aurovertins mediate interactions between the pathogenic fungus pochonia chlamydosporia and its nematode host. | nematophagous fungi are globally distributed soil fungi and well-known natural predators of soil-dwelling nematodes. pochonia chlamydosporia can be found in diverse nematode-suppressive soils as a parasite of nematode eggs and is one of the most studied potential biological control agents of nematodes. however, little is known about the functions of small molecules in the process of infection of nematodes by this parasitic fungus or about small-molecule-mediated interactions between the pathogen ... | 2015 | 26151481 |
| synthesis and nematicidal activities of 1,2,3-benzotriazin-4-one derivatives against meloidogyne incognita. | a series of novel 1,2,3-benzotriazin-4-one derivatives were synthesized by the reaction of 3-bromoalkyl-1,2,3-benzotriazin-4-ones with potassium salt of 2-cyanoimino-4-oxothiazolidine in the presence of potassium iodide. nematicidal assays in vivo showed that some of them exhibited good control efficacy against the cucumber root-knot nematode disease caused by meloidogyne incognita, up to 100% at the concentration of 10.0 mg l(-1), which indicated that 1,2,3-benzotriazin-4-one derivatives might ... | 2015 | 26145729 |
| systemic acquired resistance activation in solanaceous crops as a management strategy against root-knot nematodes. | activators of systemic acquired resistance (sar), such as salicylic acid (sa) and its synthetic functional analogues benzo(1,2,3)thiadiazole-7-carbothionic acid-s-methyl ester (bth) and 2,6-dichloroisonicotinic acid (ina), were tested on tomato, eggplant and pepper for the control of the root-knot nematode meloidogyne incognita. effects on plant fitness, nematode reproduction and root galling were screened in relation to different methods of application, to different applied dosages of chemicals ... | 2016 | 26085141 |
| nematicidal activity of the volatilome of eruca sativa on meloidogyne incognita. | research on new pesticides based on plant extracts, aimed at the development of nontoxic formulates, has recently gained increased interest. this study investigated the use of the volatilome of rucola (eruca sativa) as a powerful natural nematicidal agent against the root-knot nematode, meloidogyne incognita. analysis of the composition of the volatilome, using gc-ms-spme, showed that the compound (z)-3-hexenyl acetate was the most abundant, followed by (z)-3-hexen-1-ol and erucin, with relative ... | 2015 | 26082278 |
| proteome of soybean seed exudates contains plant defense-related proteins active against the root-knot nematode meloidogyne incognita. | several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. this study focused on the proteins released in the exudates of soybean seeds and evaluated their nematicidal properties against meloidogyne incognita. a proteomic approach indicated the existence of 63 exuded proteins, including β-1,3-glucanase, chitinase, lectin, trypsin inhibitor, and lipoxy ... | 2015 | 26034922 |
| knock-down of heat-shock protein 90 and isocitrate lyase gene expression reduced root-knot nematode reproduction. | crop losses caused by nematode infections are estimated to be valued at usd 157 billion per year. meloidogyne incognita, a root-knot nematode (rkn), is considered to be one of the most important plant pathogens due to its worldwide distribution and the austere damage it can cause to a large variety of agronomically important crops. rna interference (rnai), a gene silencing process, has proven to be a valuable biotechnology alternative method for rkn control. in this study, the rnai approach was ... | 2015 | 26020830 |
| the plant cell inhibitor krp6 is involved in multinucleation and cytokinesis disruption in giant-feeding cells induced by root-knot nematodes. | the plant cell cycle inhibitor gene krp6 has been investigated in roots infected by plant-parasitic root-knot nematodes (meloidogyne spp.). unexpectedly, krp6 overexpressing lines revealed a distinct role for this specific krp as an activator of the mitotic cell cycle. this function was confirmed in arabidopsis thaliana suspension cultures ectopically expressing krp6. a blockage in the mitotic exit was observed in cell suspensions and in giant cells resulted in the appearance of multi-nucleated ... | 2015 | 25915833 |
| tomato transgenic plants expressing hairpin construct of a nematode protease gene conferred enhanced resistance to root-knot nematodes. | root-knot nematodes (meloidogyne incognita) cause substantial yield losses in vegetables worldwide, and are difficult to manage. continuous withdrawal of environmentally-harmful nematicides from the global market warrants the need for novel nematode management strategies. utility of host-delivered rnai has been demonstrated in several plants (arabidopsis, tobacco, and soybean) that exhibited resistance against root-knot and cyst nematodes. herein, a m. incognita-specific protease gene, cathepsin ... | 2015 | 25883594 |
| development of polymorphic microsatellites for meloidogyne incognita, through screening predicted microsatellite loci based on genome sequence. | microsatellites are extensively distributed in the eukaryotic genome, and they are widely used for their high polymorphism and accessibility. the microsatellites in m. incognita, a worldwide agriculture pest, are inadequate for diversity research. a repertoire of 1620 microsatellites appeared appropriate to design primer as markers were identified based on the m. incognita genome. 120 loci were chosen as candidate, from which 88 microsatellites were characterized. finally, we found 13 polymorphi ... | 2015 | 25857200 |
| correction to nematicidal activities of 4-quinolone alkaloids isolated from the aerial part of triumfetta grandidens against meloidogyne incognita. | | 2015 | 25830595 |
| transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode meloidogyne incognita. | nematodes are one of the major limiting factors in alfalfa production. root-knot nematodes (rkn, meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. as of today, no studies have been published on global gene expression profiling in alfalfa infected with rkn or any other plant parasitic nematode. very little information is available about molecular mechanisms that contribute to ... | 2015 | 25822722 |
| associated bacteria of different life stages of meloidogyne incognita using pyrosequencing-based analysis. | the root knot nematode (rkn), meloidogyne incognita, belongs to the most damaging plant pathogens worldwide, and is able to infect almost all cultivated plants, like tomato. recent research supports the hypothesis that bacteria often associated with plant-parasitic nematodes, function as nematode parasites, symbionts, or commensal organisms etc. in this study, we explored the bacterial consortia associated with m. incognita at different developmental stages, including egg mass, adult female and ... | 2015 | 25809195 |
| cowpea-meloidogyne incognita interaction: root proteomic analysis during early stages of nematode infection. | cowpea (vigna unguiculata l. walp) is an important legume species well adapted to low fertility soils and prolonged drought periods. one of the main problems that cause severe yield losses in cowpea is the root-knot nematode meloidogyne incognita. the aim of this work was to analyze the differential expression of proteins in the contrasting cultivars of cowpea ce 31 (highly resistant) and ce 109 (slightly resistant) during early stages of m. incognita infection. cowpea roots were collected at 3, ... | 2015 | 25736976 |
| transformation of the endophytic fungus acremonium implicatum with gfp and evaluation of its biocontrol effect against meloidogyne incognita. | acremonium implicatum is an endophytic fungus with biocontrol potential against meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. to understand its mode of plant endophytism and opportunistic egg parasitism, gfp-tagged a. implicatum was constructed by peg-mediated protoplast transformation. by laser scanning confocal microscopy (lscm), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (acr-1). acr-1 ... | 2015 | 25724297 |
| transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode meloidogyne incognita. | nematodes are one of the major limiting factors in alfalfa production. root-knot nematodes (rkn, meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. as of today, no studies have been published on global gene expression profiling in alfalfa infected with rkn or any other plant parasitic nematode. very little information is available about molecular mechanisms that contribute to ... | 2015 | 25710378 |
| nematicidal activity of acetophenones and chalcones against meloidogyne incognita and structure-activity considerations. | with the ultimate goal of identifying new compounds active against root-knot nematodes, a set of 14 substituted chalcones were synthesised, starting from acetophenones. these chalcones and various acetophenones were tested in vitro against meloidogyne incognita. | 2016 | 25641877 |
| maturation of nematode-induced galls in medicago truncatula is related to water status and primary metabolism modifications. | root-knot nematodes are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. during a compatible interaction, these nematodes induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells (gcs). these metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. we analyzed the modifications of water status, ionic content and accumulation of metabolites in development and ma ... | 2015 | 25617326 |
| expression of phenylalanine ammonia lyase genes in maize lines differing in susceptibility to meloidogyne incognita. | maize is a well-known host for meloidogyne incognita, and there is substantial variation in host status among maize genotypes. in previous work it was observed that nematode reproduction increased in the moderately susceptible maize inbred line b73 when the zmlox3 gene from oxylipid metabolism was knocked out. additionally, in this mutant line, use of a nonspecific primer for phenyl alanine ammonialyase (pal) genes indicated that expression of these genes was reduced in the mutant maize plants w ... | 2014 | 25580029 |
| physiological effects of meloidogyne incognita infection on cotton genotypes with differing levels of resistance in the greenhouse. | greenhouse tests were conducted to evaluate (i) the effect of meloidogyne incognita infection in cotton on plant growth and physiology including the height-to-node ratio, chlorophyll content, dark-adapted quantum yield of photosystem ii, and leaf area; and (ii) the extent to which moderate or high levels of resistance to m. incognita influenced these effects. cultivars fibermax 960 br (susceptible to m. incognita) and stoneville 5599 br (moderately resistant) were tested together in three trials ... | 2014 | 25580028 |
| response of resistant and susceptible bell pepper (capsicum annuum) to a southern california meloidogyne incognita population from a commercial bell pepper field. | to determine the presence and level of root-knot nematode (meloidogyne spp.) infestation in southern california bell pepper (capsicum annuum) fields, soil and root samples were collected in april and may 2012 and analyzed for the presence of root-knot nematodes. the earlier samples were virtually free of root-knot nematodes, but the later samples all contained, sometimes very high numbers, of root-knot nematodes. nematodes were all identified as m. incognita. a nematode population from one of th ... | 2014 | 25580027 |
| pyramiding taro cystatin and fungal chitinase genes driven by a synthetic promoter enhances resistance in tomato to root-knot nematode meloidogyne incognita. | meloidogyne incognita, one of the major root-knot nematode (rkn) species in agriculture, attacks many plant species, causing severe economic losses. genetic engineering of plants with defense-responsive genes has been demonstrated to control rkn. these studies, however, focused on controlling rkn at certain growth stages. in the present study, a dual gene overexpression system, utilizing a plant cysteine proteinase inhibitor (cecpi) and a fungal chitinase (pjchi-1), was used to transform tomato ... | 2015 | 25575993 |
| control of meloidogyne incognita using mixtures of organic acids. | this study sought to control the root-knot nematode (rkn) meloidogyne incognita using benign organo-chemicals. second-stage juveniles (j2) of rkn were exposed to dilutions (1.0%, 0.5%, 0.2%, and 0.1%) of acetic acid (aa), lactic acid (la), and their mixtures (mx). the nematode bodies were disrupted severely and moderately by vacuolations in 0.5% of mx and single organic acids, respectively, suggesting toxicity of mx may be higher than aa and la. the mortality of j2 was 100% at all concentrations ... | 2014 | 25506312 |
| nematicidal activities of 4-quinolone alkaloids isolated from the aerial part of triumfetta grandidens against meloidogyne incognita. | the methanol extract of the aerial part of triumfetta grandidens (tiliaceae) was highly active against meloidogyne incognita, with second-stage juveniles (j2s) mortality of 100% at 500 μg/ml at 48 h post-exposure. two 4-quinolone alkaloids, waltherione e (1), a new alkaloid, and waltherione a (2), were isolated and identified as nematicidal compounds through bioassay-guided fractionation and instrumental analysis. the nematicidal activities of the isolated compounds against m. incognita were eva ... | 2015 | 25494674 |
| enhanced synthesis of antioxidant enzymes, defense proteins and leghemoglobin in rhizobium-free cowpea roots after challenging with meloydogine incognita. | the root knot nematodes (rkn), meloydogine spp., particularly meloidogyne incognita and meloidogyne javanica species, parasitize several plant species and are responsible for large annual yield losses all over the world. only a few available chemical nematicides are still authorized for rkn control owing to environmental and health reasons. thus, plant resistance is currently considered the method of choice for controlling rkn, and research performed on the molecular interactions between plants ... | 2014 | 28250394 |
| overexpression of mic-3 indicates a direct role for the mic gene family in mediating upland cotton (gossypium hirsutum) resistance to root-knot nematode (meloidogyne incognita). | transgene-based analysis of the mic-3 gene provides the first report of a cotton gene having a direct role in mediating cotton resistance to root-knot nematode. major quantitative trait loci have been mapped to upland cotton (gossypium hirsutum l.) chromosomes 11 and 14 that govern the highly resistant phenotype in response to infection by root-knot nematode (rkn; meloidogyne incognita); however, nearly nothing is known regarding the underlying molecular determinants of this rkn-resistant phenot ... | 2015 | 25376794 |
| screening and histopathological characterization of korean carrot lines for resistance to the root-knot nematode meloidogyne incognita. | in total, 170 carrot lines developed in korea were screened for resistance to meloidogyne incognita race 1 to select parental genetic resources useful for the development of nematode-resistant carrot cultivars. using the gall index (gi), gall formation was examined on carrot roots inoculated with approximately 1,000 second-stage juveniles of the nematode 7 weeks after inoculation. sixty-one carrot lines were resistant (gi ≤ 1.0), while the other 109 were susceptible (gi > 1.0) with coefficient o ... | 2014 | 25288988 |
| the feeding behavior of adult root-knot nematodes (meloidogyne incognita) in rose balsam and tomato. | meloidogyne incognita is a parasitic root-knot nematode that causes considerable yield loss in a wide range of plants. in this study we documented the movement of adult female nematodes for more than 2 hr in micro-slices of infected tomato (solanum lycopersicum) and rose balsam (impatiens balsamina) plants using light and video microscopy. stylet thrusting was followed by short pumping actions of the esophagus, dorsal esophageal gland ampulla, and metacorpal bulb. regular thrusting was normally ... | 2014 | 25276004 |
| identification of msp1 gene variants in populations of meloidogyne incognita using pcr-dgge. | effectors of root-knot nematodes are essential for parasitism and prone to recognition by adapted variants of the host plants. this selective pressure initiates hypervariability of effector genes. diversity of the gene variants within nematode populations might correlate with host preferences. in this study we developed a method to compare the distribution of variants of the effector gene msp1 among populations of meloidogyne incognita. primers were designed to amplify a 234-bp fragment of msp1. ... | 2014 | 25276001 |
| mitochondrial haplotype-based identification of ethanol-preserved root-knot nematodes from africa. | the asexual root-knot nematodes (rkns) (meloidogyne spp.) exemplified by meloidogyne incognita are widespread and damaging pests in tropical and subtropical regions worldwide. comparison of amplification products of two adjacent polymorphic regions of the mitochondrial genome using dna extracts of characterized rkn strains, including 15 different species, indicate that several species are derived from the same or closely related female lineages. nevertheless, m. javanica, m. enterolobii, m. inco ... | 2015 | 25271352 |
| nematicidal activity of paecilomyces lilacinus 6029 cultured on karanja cake medium. | antagonistic fungi parasitize root-knot nematodes through secretion of extracellular hydrolytic enzymes and secondary metabolites. in present study, in vitro bioassay showed that paecilomyces lilacinus 6029 culture filtrate from karanja cake medium killed 100% meloidogyne incognita larvae while only 78.28% mortality was recorded by czapeck-dox filtrate within 12 h of exposure. the filtrate, irrespective of culture medium, was found to be more nematotoxic when incubated for 15 days. fourier trans ... | 2014 | 25193498 |
| benzothiadiazole effect in the compatible tomato-meloidogyne incognita interaction: changes in giant cell development and priming of two root anionic peroxidases. | bth application is effective in root-knot nematode-tomato interaction in a way that involves a delay in the formation of nematode feeding site and triggers molecular responses at several levels. the compatible interaction between root-knot nematodes and their hosts requires the nematode to overcome plant defense systems so that a sophisticated permanent feeding site (giant cells) can be produced within the host roots. it has been suggested that activators of plant defenses may provide a novel ma ... | 2014 | 25085693 |
| ovicidal activity of lactic acid produced by lysobacter capsici ys1215 on eggs of root-knot nematode, meloidogyne incognita. | lysobacter capsici ys1215 isolated from soil previously showed nematicidal potential for biological control of the root-knot nematode. in this study, lactic acid, a nematicidal compound, was isolated from culture filtrate of ys1215, and its ovicidal activity was investigated. purification and identification of lactic acid were performed by a series of column chromatographies and identified by (1)h and (13)c nmr spectra and gc-ms analysis. our results showed that bacterial culture filtrate contai ... | 2014 | 25085571 |
| effect of supreme 15 cs against meloidogyne incognita on tomato. | pot and laboratory experiments were conducted to study the efficacy of supreme 15 cs in varying doses for management of root- knot nematode, m. incognita in tomato. the results indicated that supreme 15 cs containing alkali metal n-(n-propyl) dithiocarbamate proved to be more effective in improving plant growth characters and reducing root-knot index and final nematode population. among the doses tested, higher dose of 0.5 % (w/w) was more effective than lower doses. | 2014 | 25035599 |
| development of enzyme linked immunosorbent assay (elisa) for the detection of root-knot nematode meloidogyne incognita. | root-knot nematodes (meloidogyne incognita) are obligate, sedentary plant endoparasites that are extremely polyphagous in nature and cause severe economic losses in agriculture. hence, it is essential to control the parasite at an early stage. for any control strategy to be effective, an early and accurate diagnosis is of paramount importance. immunoassays have the inherent advantages of sensitivity and specificity; have the potential to identify and quantify these plant-parasitic nematodes. hen ... | 2014 | 25035590 |
| effect of simultaneous water deficit stress and meloidogyne incognita infection on cotton yield and fiber quality. | both water deficit stress and meloidogyne incognita infection can reduce cotton growth and yield, and drought can affect fiber quality, but the effect of nematodes on fiber quality is not well documented. to determine whether nematode parasitism affects fiber quality and whether the combined effects of nematode and drought stress on yield and quality are additive (independent effects), synergistic, or antagonistic, we conducted a study for 7 yr in a field infested with m. incognita. a split-plot ... | 2014 | 24987162 |
| management of meloidogyne incognita with chemicals and cultivars in cotton in a semi-arid environment. | management of meloidogyne incognita (root-knot nematode) in cotton in the united states was substantially affected by the decision to stop production of aldicarb by its principle manufacturer in 2011. the remaining commercially available tools to manage m. incognita included soil fumigation, nematicide seed treatments, postemergence nematicide application, and cultivars partially resistant to m. incognita. small plot field studies were conducted on a total of nine sites from 2011-2013 to examine ... | 2014 | 24987161 |
| tulipaline a: structure-activity aspects as a nematicide and v-atpase inhibitor. | carbonyl groups are known to form covalent adducts with endogenous proteins, but so far, their nematicidal mechanism of action of has been overlooked. the nematicidal activity of ten lactones was tested in vitro against the root knot nematodes meloidogyne incognita and meloidogynearenaria. in particular, the saturated lactones α-methylene-γ-butyrolactone or tulipaline a (1) and γ-butyrolactone (3) were active against m. incognita with an ec50/48h of 19.3±10.0 and 40.0±16.2mg/l respectively. more ... | 2014 | 24974115 |