Publications

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aureomycin in theileria parva infection. 195313025470
connective tissue reactions in acute fatal east coast fever (theileria parva) of cattle. 196013687137
attempts to adapt theileria parva to laboratory animals. 19635896628
observations on the resistance of jersey-nganda calves to east coast fever (theileria parva). 19635896629
cyclic development and longevity of theileria parva in the tick rhipicephalus appendiculatus. 196414242265
the immunisation of cattle against east coast fever (theileria parva infection) using tetracyclines: a review of the literature and a reappraisal of the method. 196514344196
the transmission of theileria parva (theiler, 1904) by rhipicephalus carnivoralis walker, 1965. 19664957988
transition of developmental stages of theileria parva in vitro at high temperature. 19664960955
a preliminary electron microscopic study of east coast fever (theileria parva infection). 19664959286
[fine structure of merozoites of theileria parva]. 19674971422
[electronmicroscopical studies of the multiplication of theileria parva in cattle]. 19674971423
preliminary experiments with antigens of theileria parva (theiler, 1904). 19675624605
a mild form of east coast fever (theileria parva infection) becoming virulent on passage through cattle. 19685652740
theileria parva: possible serological test for east coast fever. 19685726858
the development of theileria parva in the salivary glands of the tick, rhipicephalus appendiculatus. 19684984381
[serological studies on theileria parva infection in cattle]. 19684180841
the use of parasympathomimetic drugs to stimulate salivation in the tick rhipicephalus appendiculatus, and the transmission of theileria parva using saliva obtained by this method from infected ticks. 19694986780
theileria parva: kinetics of replication. 19694975359
serological response in cattle to east coast fever (theileria parva infection) as measured by the indirect fluorescent antibody test. 19694902152
ifection of rhipicephalus appendiculatus ticks with theileria parva, using a rabbit's head perfusion technique. 19705498934
infection of the tick rhipicephalus appendiculatus with theileria parva using an artificial feeding technique. 19705499333
theileria parva: comparative infection rates of adult and nymphal rhipicephalus appendiculatus. 19714995423
the fine structure of tick stages of theileria parva. 19715002523
application of the indirect fluorescent antibody test in experimental east coast fever (theileria parva infection of cattle). 19714934252
transformation of reticulum cells to lymphoblasts in cultures of bovine spleen infected with theileria parva. 19714323780
growth characteristics of theileria parva-infected bovine lymphoblast cultures. 19714106174
duration of immunity to east coast fever (theileria parva infection of cattle). 19725039345
east coast fever: field challenge of cattle immunised against theileria parva (muguga). 19724210445
uptake of 3 h-thymidine during artificial feeding of rhipicephalus appendiculatus ticks infected with theileria parva. 19724622849
growth of theileria parva-infected bovine lymphoid cells in irradiated mice. 19724502803
the indirect fluorescent antibody test for experimental east coast fever (theileria parva infection of cattle). evaluatin of dried blood samples as a source of antibody. 19724556468
a capillary-tube agglutination test for the detection and titration of theileria parva and theileria mutans antibodies in bovine serum. 19724564332
the indirect fluorescent antibody test for experimental east coast fever (theileria parva infection of cattle). evaluation of a cell culture schizont antigen. 19724564334
some effects of irradiation on theileria parva in the salivary glands of the tick rhipicephalus appendiculatus. 19724626453
the duration of emission of infective particles of theileria parva by infected ticks fed artificially. 19724627336
the indirect fluorescent antibody test for experimental east coast fever (theileria parva infection of cattle). detection of antibodies to schizonts in dried blood samples. 19724628843
attempts to grow tissue culture cells, infected with theileria parva, in laboratory animals. 19724630814
east coast fever of cattle: 60 co irradiation of infective particles of theileria parva. 19734196860
east coast fever: correlation between the morphology and infectivity of theileria parva developing in its tick vector. 19734198658
east coast fever: the infectivity for cattle of infective particles of theileria parva harvested in various substrates. 19734200007
cryopreservation of infective particles of theileria parva. 19734200405
letter: infection and transformation of bovine lymphoid cells in vitro by infective particles of theileria parva. 19734200607
studies on colostral antibodies to theileria parva using the indirect fluorescent antibody test. 19734200673
observations on the cross-immunity between theileria lawrencei (serengeti) and theileria parva (muguga) in cattle. 19734202666
duration of serological response to the indirect fluorescent antibody test of cattle recovered from theileria parva infection. 19734217920
the use of fluorescent antibody technqiues to detect theileria parva in the salivary glands of the tick rhipicephalus appendicalatus. 19734196560
responses of the bovine lymphatic system to infection by theileria parva. i. histology and ultrastructure of lymph nodes in experimentally-infected calves. 19734199457
responses of the bovine lymphatic system to infection by theileria parva. ii. changes in the central lymph in experimentally-infected calves. 19734199458
theileria parva: comparative infectivity of a ground tick stabilate and a classical 10-tick challenge. 19734215109
some experiments on the transmission of theileria mutans (theiler, 1906) and theileria parva (theiler, 1904) by the ticks amblyomma variegatum (fabricius, 1794) and rhipicephalus appendiculatus neumann, 1901, in uganda. 19744607395
response of whole-body irradiated mice to inoculation of theileria parva-infected bovine lymphoid cells. 19744218245
east coast fever: 60co-irradiation of theileria parva in its tick vector, rhipicephalus appendiculatus. 19744213045
east coast fever: quantitative studies of theileria parva in cattle. 19744213046
the specific immunoglobulin response in cattle immunized with isolated theileria parva antigens. 19744213482
the specific immunoglobulin response in cattle immunized with theileria parva (muguga) stabilate. 19744213483
theileria parva: variation in the infection rate of the vector tick, rhipicephalus appendiculatus. 19744213496
east coast fever: titration in cattle of suspensions of theileria parva derived from ticks. 19744214585
growth of theileria parva-infected bovine lymphoid cells in whole-body irradiated mice. 19744206061
immunochemical studies on east coast fever. i. partial segregation and characterization of the theileria parva schizont antigen. 19744214909
immunochemical studies on east coast fever. ii. partial segregation and characterization of the theileria parva piroplasm antigen. 19744214910
immunochemical studies on east coast fever. iii. development of an indirect hemagglutination assay using theileria parva piroplasm antigen. 19744214911
establishment of theileria parva-infected lymphoblastoid cell lines using homologous feeder layers. 19744856520
cell fusion, using sendai virus, to effect inter-species transfer of a cell-associated parasite (theileria parva). 19744369833
effect of humoral antibodies on the course of theileria parva infection (east coast fever) of cattle.a study was made of the effects of serum and gamma-globulins from vaccinated cattle (donor cattle) given to cattle (principals) exposed to theileria parva infection (east coast fever (ecf)). the principals were treated with normal serum, with serum containing high antibody titer against t parva (as evaluated by the indirect fluorescent antibody (ifa) technique), or with globulins prepared from normal and from immune serums. for exposure, the principals were inoculated with standardized suspensio ...197547727
heterotransplantation of theileria parva-infected cells to athymic mice. 1975804142
the specific immunoglobulin response in cattle to theileria parva (muguga) infection.cattle were inoculated with dilutions of theileria parva (muguga) stabilate without concurrent antibiotic treatment. the dilutions were selected to induce severe east coast fever infections, which in some cases would lead to death. serum samples were collected and the complement fixation (cf), indirect haemagglutination (iha) and indirect immunofluorescence (ifa) tests were used to detect t. parva serum antibodies. sera were fractionated on sephadex g200 to determine the activities of specific i ...1975804155
the effect of 3, 6, bis-dimethylaminoacridine on cultured bovine lymphoid cells infected with theileria parva and in cattle experimentally infected with east coast fever. 1975805506
theileria parva: effects of irradiation on a culture of parasitized bovine lymphoid cells. 1975807485
hybrid cells, infected with theileria parva, formed by fusion of hamster and mouse cells with parasitised bovine lymphoid cells.bovine lymphoid cells from a culture line parasitised with theileria parva were fused to mouse heart (mh) and baby hamster kidney (bhk) cells, grown as monolayers, using inactivated sendai virus. the resultant heterokaryons contained macroschizonts of t parva. macroschizonts also occurred in cells that apparently contained only bhk or mh nuclei. parasites underwent varying degrees of development; in some cases microschizonts resulted, and in other cases massive atypical parasite masses were form ...1975809820
the effect of leucocytes from cattle immunized against east coast fever on the migration of bovine lymphoblasts infected with theileria parva. 1975812298
comparative growth of bovine lymphosarcoma cells and lymphoid cells infected with theileria parva in athymic (nude) mice. 19751173457
adaptation and possible attenuation of theileria parva-infected cells grown in irradiated mice. 1976815278
simulation in vitro of bovine host cycle of theileria parva. 1976818570
attempts to produce theileria parva-infected mouse cells using cell fusion techniques.ehrlich ascites tumour (eat) cells were fused with theileria parva-infected bovine lymphoid (c2) cells using inactivated sendai virus. a variety of techniques were employed to try and increase fusion percentage and harvest of heterokaryons. there was no evidence that pre-exposure of c2 cells to sendai virus or phytohaemagglutinin improved fusion percentage, but the percentage was higher when 10(8) c2 cells were fused with 10(7) eat cells, as compared with a ratio of 10(7) c2: 10(6) eat. an incre ...1976184506
[electron microscope studies on developmental stages of theileria parva (theiler, 1904) in the intestine of the tick hyalomma anatolicum excavatum (koch, 1844) (author's transl)].the developmental stages of theileria parva within the intestine of ticks were studied by electron microscopy. on the 2nd-4th day post repletionem (p.r.) the intestine of the ticks contained numerous stages, which were very similar to microgamonts and microgametes of haemosporidia. from these results it seemed likely that gamogony of theileria parva takes place in the intestine of ticks, too. the microgamont-like stages were spear-head-like; they measured about 10.5 mum in length with a maximum ...1976821188
chemotherapy of theileria parva infection. 1976934316
the effect of calf lymph and bovine red blood cells on in vitro cultivation of theileria parva-infected lymphoid cells.calf lymph introduced to cultures of theileria parva-infected lymphoid cells caused a stimulation of both cells and parasites resulting in the formation of rapidly dividing cells and the developmental stages of the parasite. the presence of piroplasms was confirmed when washed bovine red blood corpuscles were added to the culture1976968954
studies on theileriidae (sporozoa) in tanzania. iv. a field trial on immunization against east coast fever (theileria parva infection of cattle).seven cattle, immunized by an infection and treatment method with 3 strains of theileria parva (muguga, kiambu 5 and serengeti transformed), and five controls were exposed to natural tick infestation in tanzania, at a site where 2 precontrols had previously contracted fatal east coast fever within 3 weeks. all controls became infected with fatal ecf within a month of exposure. one immunized animal survived, two died from a theileria mutans infection and one from a babesia bigemina infection. the ...1976982549
an ultrastructural study of heterokaryons derived from theileria parva-infected bovine lymphoblasts and ehrlich ascites tumour cells.an ultrastructural study was made of sendai virus induced heterokaryons derived from theileria parva-infected lymphoblasts and ehrlich ascites tumour cells. when fusion occurred, parasites were successfully integrated into the cytoplasm of the resulting heterokaryons where they appeared as morphologically normal macroschizonts. homokaryon formation was also noted. this occurred frequently between ehrlich ascites tumour cells and rarely with lymphoblasts. a small proportion of heterokaryons conta ...1976987607
studies on theileriidae (sporozoa) in tanzania. v. preliminary experiments on a new method for infecting ticks with theileria parva and theileria mutans.bovine blood with a high parasitaemia of theileria mutans was injected through the cuticle into fully engorged nymphs of amblyomma variegatum which had recently detached from a rabbit. after moulting, they transmitted this parasite to a susceptible calf. rhipicephalus appendiculatus transmitted theileria parva in the same way, but results were negative with a. variegatum and t. parva. this method of infecting ticks appears promising for the isolation of theileria strains from the field, and migh ...19761006800
immunization of cattle against theileria parva. 1977596297
the serological relationship between theileria parva (muguga) and theileria lawrence from rhodesia.thirty-eight sera from cattle from a herd in rhodesia naturally infected with theileria lawrencei were titrated with the indirect immunofluorescent test against schizont antigens of t parva (muguga) and t lawrencei from rhodesia. t lawrencei was found to be indistinguishable from t parva with the test.1977878256
immunization against east coast fever (theileria parva infection of cattle) by infection and treatment: chemoprophylaxis with n-pyrrolidinomethyl tetracycline.cattle were immunized against east coast fever by the inoculation of a tick-derived stabilate of theileria parva infective particles and the intramuscular injection, at daily intervals, of n-pyrrolidinomethyl tetracycline at a dose level of 5 mg/kg. four doses of the tetracycline, starting on the day of inoculation of the infective stabilate, minimised the ecf reactions in the cattle during immunization. immunity to homologous t. parva stabilate challenge was demonstrated in 27 out of 27 cattle ...1977910283
theileria parva: purine and pyrimidine metabolism and the action of folate antagonists in parasitized bovine lymphoid cells. 1977138597
development of theileria parva (theiler, 1904) in the gut of rhipicephalus appendiculatus (neumann, 1901). 1977415285
the use of tetracyclines on the chemotherapy of experimental east coast fever (theileria parva infection of cattle).in a series of experiments, groups of cattle, undergoing patent east coast fever (ecf) reactions induced by the inoculation of tick-derived stabilates of theileria parva infective particles, were treated by parenteral administration of 2 tetracyclines. ten cattle were treated with n-pyrrolidinomethyl tetracycline at 15 mg/kg for 5 days, starting on the first day macroschizonts were detected in lymph node biopsy smears. nine of these treated cattle survived ecf reactions induced by an inoculum wh ...1977414390
theileria parva piroplasmosis in mice, and its attempted transmission with rhipicephalus appendiculatus ticks.theileria parva piroplasm infection of mouse erythrocytes was detected, following intraperitoneal inoculation of mice with irradiated cultures of t. parva-infected bovine lymphoid cells. infection could not be transmitted to cattle using rhipicephalus appendiculatus ticks.1977414389
studies on theileriidae (sporozoa) in tanzania. x. a large-scale field trial on immunization against cattle theileriosis.50 cattle immunized by an infection and treatment method with 3 strains of theileria parva (mugaga, kiambu 5 and serengeti transformed), together with 19 controls, were exposed to natural tick infestation in tanzania, in a site where a continuous influx of infected ticks from different regions of the country may be expected to occur. exposure lasted for 2 months, monitoring continued for 3 more weeks after exposure ended. all 19 controls contracted east coast fever and died. 40 of the 50 immuniz ...1977414388
east coast fever immunisation trials in uganda: field exposure of zebu cattle immunized with three isolates of theileria parva. 1977413226
studies on theileriidae (sporozoa) in tanzania. viii. experiments with african buffalo (syncerus caffer).it has been found that african buffalo may remain infective carriers of theileria parva lawrencei for at least 5 years. this infection is now known to exist in buffalo in 3 sites in northern tanzania. it was shown that buffalo can be infected with haematoxenus veliferus and theileria mutans of cattle and retransmission of these parasites from buffalo to cattle was successful. the species of haematoxemus reported in wild buffalo in central and east africa is likely to be h. veliferus, while the p ...1977410128
studies on theileriidae (sporozoa) in tanzania. vii. additional note on the transmission of theileria parva.rhipicephalus evertsi successfully transmitted fatal east coast fever (theileria parva infection) in one experiment. a second one gave negative results, as did an attempted transmission of t. parva by amblyomma variegatum.1977407682
detection of antibodies in trypanosome-infected cattle by means of a microplate enzyme-linked immunosorbent assay.a micromodification of the enzyme-linked immunosorbent assay (elisa) was evaluated for its potential application in the immunodiagonsis of bovine trypanosomiasis. serum samples from infected and non-infected zebu cattle and samples from friesian cattle with experimental infections were exmained for the presence of trypanosomal antibodies. there were significant differences between the microelisa values obtained with samples from infected and non-infected cattle. during the course of infection mi ...1977333677
studies on theileriidae (sporozoa) in tanzania. vi. second field trial on immunization against cattle theileriosis.nine cattle, immunized by an infection and treatment method with 3 strains of theileria parva (muguga, kiambu 5 and serengeti transformed) and nine controls, were exposed to natural tick infestation in tanzania. all controls contracted fatal east coast fever within 2 months of exposure. all immunized animals survived the period of exposure (over 2 months), but later one died of ecf, one of heart-water, one of an undetermined cause (not a theileriosis), and one disappeared from the herd. the 5 re ...1977324053
purification and characterization of precipitating antigens from theileria parva.precipitating antigens from theileria parva have been partially purified. two antigens from each of the schizont and piroplasm stages of the parasite were identified; the major antigens from the two stages shared the same specificity. the antigens showed considerable molecular heterogeneity, almost certainly a result of the preparative method, and they always contained large amounts of dna. the piroplasm antigens were of parasite nuclear origin and the schizont antigens were probably of the same ...197770504
east coast fever: cultivation in vitro of cell-free schizonts and merozoites of theileria parva and their immunogenicity in cattle.cell-free schizonts and merozoites of theileria parva and theileria lawrencei, derived from parasitized lymphoid cell lines, were propagated in vitro using enriched medium. use of radioisotopic markers showed that the bovine cell-independent parasites passed through a limited but marked replication by day 4. if normal bovine rbc were inoculated in vitro with the cell-free merozoites, development of piroplasms in the rbc occurred. electron microscopic studies of the merozoites and piroplasms reve ...1978415644
initial studies on the properties of a bovine lymphoid cell culture line infected with theileria parva. 1978154370
electron microscopic studies on the development of kinetes of theileria parva theiler, 1904 in the gut of the vector ticks rhipicephalus appendiculatus neumann, 1901.the development of motile stages, called kinetes, from a stationary stage (regarded as zygote) has been followed in theileria parva by means of electron miscroscopy. this process started after moult of the tick nymphs which had sucked on highly infected calves, i.e. about 20 days after repletion (a.r.) of the ticks. the transformation took place by formation of a growing protrusion (= anlage) into an inner, enlarging vacuole. during this process the limiting membrane of the enlarging vacuole ser ...197828653
in vitro studies on the action of menoctone and other compounds on theileria parva and t. annulata.bovine lymphoblastoid cell cultures permanently infected with theileria parva and t. annulata were used in an in vitro screen to test a wide range of compounds for chemotherapeutic activity against these parasites. only two compounds, menoctone and methotrexate, showed significant activity, which has subsequently been confirmed by tests in infected cattle. menoctone was about 100 times more active than methotrexate. mixtures of menoctone and various other other compounds were tested, but showed ...1978104669
antibody-dependent cell-mediated cytotoxicity in cattle: activity against 51cr-labeled chicken erythrocytes coated with protozoal antigens.bovine mononuclear cells in the presence of bovine anti-chicken erythrocyte sera at high dilutions induce release of chromium-51 from labeled chicken erythrocytes. bovine effector cells are capable of recognizing both bovine immunoglobulin g(1) and bovine immunoglobulin g(2); in contrast, human effector cells only recognize immunoglobulin g(1). effector cell activity of bovine mononuclear cells is equally distributed between peripheral blood and spleen. as in other species, thymus and lymph node ...1978103832
histopathologic and electron microscopic studies of cutaneous lesions in calves with experimentally induced east coast fever (theileriosis).skin lesions of bovine east coast fever were examined by light and electron microscopy at 120 hours after attachment of rhipicephalus appendiculatus ticks infected with theileria parva. lesions included epidermal ulcer, hemorrhage, edema, necrosis, and inflammatory cell infiltration by mostly polymorphonuclear and mononuclear (lymphoid) cells. the trophozoite state of t parva was observed in a parasitophorous vacuole and feeding on neutrophil granules. schizonts, some in budding process, and mer ...197898077
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