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microbial metabolites with insecticidal properties.a screen of fungi for insecticidal activity revealed the ability of aspergillus versicolor to make versimide, methyl-alpha-(methylsuccinimido)acrylate, a novel contact insecticide. the larvicidal activities of alternaria tenuis and fusarium lateritium were found to be due to tenuazonic acid and diacetoxyscirpenol, respectively. thiolutin, cycloheximide, rubratoxin, patulin, trichothecin, an actinomycin, and scirpene-producing fungi also had insecticidal activity.19724628800
[ribonuclease fl1 from fusarium lateriticum. isolation, substrate specificity and amino acid sequence].extracellular rnase fl1 has been purified from the culture filtrate of fusarium lateritium. the enzyme has been obtained in the electrophoretically homogeneous state with the yield about 90% and 300 fdd degree of purification. rnase fl1 is a guanyl specific enzyme (ec 3.1.27.3) with the specific activity on rna 1420 units/mg of protein. the total primary structure of the rnase has been determined by the automated edman degradation of two non-fractionated peptide hydrolysates produced by trypsin ...19883142486
[a new isoform of intracellular rnaase of fl2 fusarium lateritium. characteristics and determining the primary structure]. 19892806059
enniatin synthetases from different fusaria exhibiting distinct amino acid specificities.enniatin synthetases from the cyclodepsipeptide producers fusarium lateritium and fusarium sambucinum were purified to homogeneity and characterized. like the previously described enniatin synthetase from fusarium scirpi both enzymes consist of a single polypeptide chain and are very similar concerning their mr (250 kdaltons) and reaction mechanism. limited proteolytic digests show only slight differences in their patterns in sds-gels. interestingly the synthetases differ in their amino acids sp ...19921399848
purification and properties of cyanide hydratase from fusarium lateritium and analysis of the corresponding chy1 gene.the filamentous fungus fusarium lateritium is cyanide tolerant, due, at least in part, to the induction by cyanide of the enzyme formamide hydrolyase (ec 4.2.1.66). this enzyme, more commonly known as cyanide hydratase, catalyses the hydration of cyanide to formamide. the enzyme was purified from f. lateritium and showed a subunit molecular mass of 43 kda (as judged by sds-page), while the native protein appeared to form aggregates of up to 1217 kda (as judged by gel-filtration and non-denaturin ...19938409923
expression of the cyanide hydratase enzyme from fusarium lateritium in escherichia coli and identification of an essential cysteine residue.the filamentous fungus fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. this enzyme catalyses the hydration of cyanide to formamide. the expression in escherichia coli of a cdna clone encoding cyanide hydratase is described. the cdna cloned was expressed as a transcriptional fusion in the expression vector pkk233-2 and a high level of activity of cyanide hydratase was detected in e. coli. site-directed mutagenesis of ...19958586260
identification and retting efficiencies of fungi isolated from dew-retted flax in the united states and europe.seven strains of filamentous fungi and one yeast were isolated from flax that was dew retted in the united states. these filamentous fungi were subcultured to purity and identified, and six appear not to have been reported earlier as isolates from dew-retted flax. five of the purified u.s. strains, two fungi isolated from flax that was dew retted in europe, and a laboratory culture of aspergillus sojae were tested for their ability to ret flax stems. the monocultures were evaluated for the degre ...199716535708
cloning and expression of a gene encoding cyanidase from pseudomonas stutzeri ak61.the gene coding for cyanidase, which catalyzes the hydrolysis of cyanide to formate and ammonia, was cloned from chromosomal dna of pseudomonas stutzeri ak61 into escherichia coli. the cyanidase gene consisted of an open reading frame of 1004 bp, and it was predicted that cyanidase was composed of 334 amino acids with a calculated molecular mass of 37,518 da. the amino acid sequence of cyanidase showed a 35.1% and 26.4% homology to aliphatic nitrilase from rhodococcus rhodochrous k22 and cyanide ...19989720205
in vivo characterization of dimethylsulfoniopropionate lyase in the fungus fusarium lateritium.a fungus, fusarium lateritium, with dimethylsulfoniopropionate (dmsp) lyase activity was isolated from both seawater and a salt marsh due to its ability to grow on dmsp (with the evolution of dimethyl sulfide) as the sole source of carbon. this is the first reported case of dmsp lyase activity in a fungus. several other common fungal genera tested did not have dmsp lyase activity. dmsp was taken up more rapidly by f. lateritium than it was utilized, leading to its intracellular accumulation. inh ...199816349475
[an alternative to conventional antibiotic therapy in respiratory infections--bioparox spray].as the main target of influenza viral aggression, the respiratory tract is subject to easier bacterial infection superimposition. the researchers from les laboratoires servier--france, managed to isolate a substance--fusafungine--from the microspore of the fungus fusarium lateritium, which demonstrates unique anti-inflammatory and antibiotic action, and is the active ingredient of bioparox spray, an inhalant. the principal indications of bioparox spray for treatment of respiratory tract infectio ...199910989675
characterisation of a cyanide hydratase gene in the phytopathogenic fungus leptosphaeria maculans.a gene encoding a cyanide hydratase was cloned from an aggressive isolate of leptosphaeria maculans, the fungus which causes blackleg disease of oilseed brassica spp. this enzyme catalyses the breakdown of hydrogen cyanide to a less toxic compound, formamide. the predicted amino acid sequence of cyanide hydratase in l. maculans is 77% and 82% identical to cyanide hydratases from two other ascomycetes, gloeocercospora sorghi and fusarium lateritium, respectively. the gene is present as a single c ...200010821180
[the hypolipidemic action of antibiotic 86/88 (enniatin b) in a hepatoblastoma g2 cell culture].a cyclodepsipeptide antibiotic 86/88 (enniatin b) with strong hypolipidemic action was isolated from the culture liquid of the fungus ina f-86/88 identified as fusarium lateritium nees var. stilboides (wr.) bilai. in the hep g2 cell culture the antibiotic suppressed 14c-acetate incorporation into cholesterol (ic50 1.75 microm), cholesterol ethers (ic50 1 microm), triglycerides (ic50 1.3 microm) and free fatty acids (ic50 2.2 microm). the most pronounced effect of the drugs, i.e. the suppression ...200010851642
characterizing the production of a wild-type and benomyl-resistant fusarium lateritium for biocontrol of eutypa lata on grapevine.benomyl-resistant (br) and wild-type (wt) strains of fusarium lateritium were examined for their tolerance to benomyl on potato dextrose agar (pda) containing benomyl and control of the eutypa lata in grapevine bioassays. the wt strain grew on pda containing 1 microg/ml benomyl at 13, 26 and 29 degrees c. the br strain grew on pda containing 10 microg/ml benomyl at 4 degrees c, on pda containing 100 microg/ml benomyl at 29 degrees c, and on pda containing 1,000 microg/ml benomyl at 13 degrees c ...200111420655
substrate-regulated cyanide hydratase (chy) gene expression in fusarium solani: the potential of a transcription-based assay for monitoring the biotransformation of cyanide complexes.the fungus fusarium solani detoxifies cyanide through induction of the cyanide hydratase gene activity (chy) in the presence of either kcn or the metal-complexed cyanides, k2ni(cn)4 or k4fe(cn)6, at ph 7.0 and 4.0 respectively. sequence analysis of the chy gene identified primers for reverse transcriptase-polymerase chain reaction (rt-pcr)-directed analysis of mrna transcripts, which demonstrated that activity correlated to the substrate-specific induction of gene expression. chy transcription w ...200212000318
canker and wilt of black locust (robinia pseudoacacia l.) caused by fusarium species.from the pathological material of black locust trees showing symptoms of wilting of the foliage or canker of the bark the following fusarium species were isolated: fusarium avenaceum (fr.) sacc., fusarium lateritium nees., fusarium semitectum berk. & rav., fusarium solani (mart.) sacc., fusarium sulphureum schlecht. (syn.: fusarium sambucinum fuckel f. 6 wollenw.) the results of the provocation infections of one-year-old black locust seedlings showed that all of the species--except fusarium sola ...200212109155
the cyanide hydratase enzyme of fusarium lateritium also has nitrilase activity.the filamentous fungus fusarium lateritium produces cyanide hydratase when grown in the presence of cyanide. the cyanide hydratase protein produced at a high level in escherichia coli shows a low but significant nitrilase activity with acetonitrile, propionitrile and benzonitrile. the nitrilase activity is sufficient for growth of the recombinant strain on acetonitrile, propionitrile or benzonitrile as the sole source of nitrogen. the recombinant enzyme shows highest nitrilase activity with benz ...200312725921
design and evaluation of a grapevine pruner for biofungicide application.eutypa lata is a significant grapevine pathogen with limited means of prevention and control. the biological control agent fusarium lateritium can prevent e. lata infection if applied directly onto a vine pruning wound. f. lateritium was suspended and stored in an invert emulsion formulation. a commercially available pruning shear was modified to dispense the formulated f. lateritium onto the cutting blade for direct application onto the pruning wound simultaneously with grapevine cutting. the m ...200515627568
gibberella xylarioides (anamorph: fusarium xylarioides), a causative agent of coffee wilt disease in africa, is a previously unrecognized member of the g. fujikuroi species complex.tracheomycosis or coffee wilt has emerged as a major disease of robusta coffee in uganda in the past 10 years. coffee wilt historically has been associated with fusarium xylarioides steyaert (teleomorph gibberella xylarioides heim and sacc.), a species that has been classified as a member of fusarium section lateritium. we investigated the molecular phylogenetics of fusarial coffee wilt isolates by generating partial dna sequences from two protein coding regions, translation elongation factor 1- ...200516389971
transformation of eutypa dieback and esca disease pathogen toxins by antagonistic fungal strains reveals a second detoxification pathway not present in vitis vinifera.eutypine, 4-hydroxybenzaldehyde, and 3-phenyllactic acid are some of the phytotoxins produced by the pathogens causing eutypa dieback and esca disease, two trunk diseases of grapevine (vitis vinifera). known biocontrol agents such as fusarium lateritium and trichoderma sp. were screened for their ability to consume these toxins. transformation time courses were performed, and an high-performance liquid chromatography-based method was developed to analyze toxin metabolism and to identify and quan ...200516131109
bioconversion of olive-mill dry residue by fusarium lateritium and subsequent impact on its phytotoxicity.the present study investigated the ability of the non-pathogenic fungus fusarium lateritium to either degrade or modify aromatic substances in olive-mill dry residue (dor) and to reduce its phytotoxicity. the 80% reduction of ethylacetate extractable phenols in dor colonized by the fungus for 20 weeks appeared to be due to polymerization reactions of phenol molecules as suggested by mass-balance ultrafiltration and size-exclusion chromatography experiments. several lignin-modifying oxidases, inc ...200516054908
isolation, characterization and cloning of a cdna encoding a new antifungal defensin from phaseolus vulgaris l. seeds.the pvd1 defensin was purified from phaseolus vulgaris (cv. pérola) seeds, basically as described by terras et al. [terras frg, schoofs hme, de bolle mfc, van leuven f, ress sb, vanderleyden j, cammue bpa, broekaer twf. analysis of two novel classes of plant antifungal proteins from radish (raphanus sativus l.) seeds. j biol chem 1992;267(22):15301-9], with some modifications. a deae-sepharose, equilibrated with 20mm tris-hcl, ph 8.0, was initially utilized for the separation of peptides after a ...200818786582
investigation of fatty acid elongation and desaturation steps in fusarium lateritium by quantitative two-dimensional deuterium nmr spectroscopy in chiral oriented media.the origin of hydrogen atoms during fatty acid biosynthesis in fusarium lateritium has been quantified by isotope tracking close to natural abundance. methyl linoleate was isolated from f. lateritium grown in natural abundance medium or in medium slightly enriched with labeled water, glucose, or acetate, and the (2)h incorporation was determined by quantitative (2)h-{(1)h} nmr in isotropic and chiral oriented solvents. thus, the individual ((2)h/(1)h)(i) ratio at each pro-r and pro-s hydrogen po ...200919213728
morphological and molecular analysis of fusarium lateritium, the cause of gray necrosis of hazelnut fruit in italy.fusarium lateritium is a globally distributed plant pathogen. it was recently reported as the causal agent of nut gray necrosis (ngn) on hazelnut. isolate characterization within f. lateritium was undertaken to investigate how morphological and molecular diversity was associated with host and geographic origin. morphological studies combined with inter-simple-sequence repeat (issr) analysis, and phylogenetic analyses using translation elongation factor 1α (tef-1α), β-tubulin genes, and nuclear r ...201121261470
[diversity and community structure of endophytic fungi from taxus chinensis var. mairei].a total of 628 endophytic fungi were isolated from 480 tissue segments of needles and branches of taxus chinensis var. mairei. according to morphological characteristics and its sequences, they represented 43 taxa in 28 genera, of which 10 hyphomycetes, 20 coelomycetes, 12 ascomycetes and 1 unknown fungus. phomopsis mali was confirmed as the dominant species. in accordance with relative frequency, alternaria alternata, aureobasidium pullulans, colletotrichum boninense, c. gloeosporioides, epicoc ...201425345060
endophytic fungi from medicinal plant bauhinia forficata: diversity and biotechnological potential.bauhinia forficata is native to south america and used with relative success in the folk medicine in brazil. the diversity, antibacterial activity, and extracellular hydrolytic enzymes of endophytic fungi associated with this plant were studied. plant samples, which included leaves, sepals, stems, and seeds, were used. ninety-five endophytic fungal were isolated (18 from leaves, 22 from sepals, 46 from stems, and nine from seeds), comprising 28 species. the most frequently isolated species were ...201526221088
purification, biochemical characterization, and antimicrobial activity of a new lipid transfer protein from coffea canephora seeds.coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. we isolated and immunolocalized cc-ltp2, a new lipid transfer protein (ltp) from coffea canephora seeds. we report its antimicrobial activity against various phytopathogenic fungi of economic importance, and against the bacterium xanthomonas euvesica ...201627813588
two new pyripyropenes from the marine fungus fusarium lateritium 2016f18-1.two new pyripyropenes, 13-dehydroxy-1,11-deacetylpyripyropene a (1) and 1-deacetylpyripyropene a (2), together with six known compounds, were isolated from a marine fungus fusarium lateritium 2016f18-1 which was associated with the sponge phyllospongia foliascens. their structures were established mainly based on nmr and ms data. their cytotoxic activities against human cancer cells cne1, cne2, hone1, sune1, glc82, and hl7702 were evaluated.201727804218
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