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differential responses of vanilla accessions to root rot and colonization by fusarium oxysporum f. sp. radicis-vanillae.root and stem rot (rsr) disease caused by fusarium oxysporum f. sp. radicis-vanillae (forv) is the most damaging disease of vanilla (vanilla planifolia and v. × tahitensis, orchidaceae). breeding programs aimed at developing resistant vanilla varieties are hampered by the scarcity of sources of resistance to rsr and insufficient knowledge about the histopathology of forv. in this work we have (i) identified new genetic resources resistant to rsr including v. planifolia inbreds and vanilla relati ...201526734032
sequence composition of bac clones and ssr markers mapped to upland cotton chromosomes 11 and 21 targeting resistance to soil-borne pathogens.genetic and physical framework mapping in cotton (gossypium spp.) were used to discover putative gene sequences involved in resistance to common soil-borne pathogens. chromosome (chr) 11 and its homoeologous chr 21 of upland cotton (g. hirsutum) are foci for discovery of resistance (r) or pathogen-induced r (pr) genes underlying qtls involved in response to root-knot nematode (meloidogyne incognita), reniform nematode (rotylenchulus reniformis), fusarium wilt (fusarium oxysporum f.sp. vasinfectu ...201526483808
the role of pathogen-secreted proteins in fungal vascular wilt diseases.a limited number of fungi can cause wilting disease in plants through colonization of the vascular system, the most well-known being verticillium dahliae and fusarium oxysporum. like all pathogenic microorganisms, vascular wilt fungi secrete proteins during host colonization. whole-genome sequencing and proteomics screens have identified many of these proteins, including small, usually cysteine-rich proteins, necrosis-inducing proteins and enzymes. gene deletion experiments have provided evidenc ...201526473835
diversity of laccase-coding genes in fusarium oxysporum genomes.multiple studies confirm laccase role in fungal pathogenicity and lignocellulose degradation. in spite of broad genomic research, laccases from plant wilt pathogen fusarium oxysporum are still not characterized. the study aimed to identify f. oxysporum genes that may encode laccases sensu stricto and to characterize the proteins in silico in order to facilitate further research on their impact on the mentioned processes. twelve sequenced f. oxysporum genomes available on broad institute of harva ...201526441870
discovery of a novel linoleate dioxygenase of fusarium oxysporum and linoleate diol synthase of colletotrichum graminicola.fungal pathogens constitute serious threats for many forms of life. the pathogenic fungi fusarium and colletotrichum and their formae speciales (f. spp.) infect many types of crops with severe consequences and fusarium oxysporum can also induce keratitis and allergic conditions in humans. these fungi code for homologues of dioxygenase-cytochrome p450 (dox-cyp) fusion proteins of the animal heme peroxidase (cyclooxygenase) superfamily. the objective was to characterize the enzymatic activities of ...201526438098
arabidopsis incurvata2 regulates salicylic acid and abscisic acid signaling, and oxidative stress responses.epigenetic regulatory states can persist through mitosis and meiosis, but the connection between chromatin structure and dna replication remains unclear. arabidopsis incurvata2 (icu2) encodes the catalytic subunit of dna polymerase α, and null alleles of icu2 have an embryo-lethal phenotype. analysis of icu2-1, a hypomorphic allele of icu2, demonstrated that icu2 functions in chromatin-mediated cellular memory; icu2-1 strongly impairs icu2 function in the maintenance of repressive epigenetic mar ...201526423959
non-random species loss in bacterial communities reduces antifungal volatile production.the contribution of low-abundance microbial species to soil ecosystems is easily overlooked because there is considerable overlap between metabolic abilities (functional redundancy) of dominant and subordinate microbial species. here we studied how loss of less abundant soil bacteria affected the production of antifungal volatiles, an important factor in the natural control of soil-borne pathogenic fungi. we provide novel empirical evidence that the loss of soil bacterial species leads to a decl ...201526405729
the effect of d123 wheat as a companion crop on soil enzyme activities, microbial biomass and microbial communities in the rhizosphere of watermelon.the growth of watermelon is often threatened by fusarium oxysporum f. sp. niveum (fon) in successively monocultured soil, which results in economic loss. the objective of this study was to investigate the effect of d123 wheat as a companion crop on soil enzyme activities, microbial biomass and microbial communities in the rhizosphere of watermelon and to explore the relationship between the effect and the incidence of wilt caused by fon. the results showed that the activities of soil polyphenol ...201526388851
role of antagonistic microorganisms and organic amendment in stimulating the defense system of okra against root rotting fungi.without application of chemical pesticides control of soilborne diseases is a great challenge. stimulation of natural plant's defense is considered as one of the most promising alternative strategy for crop protection. organic amendment of soil besides direct suppressing the pathogen, has been reported to have an influence on phytochemicals in plants. in the present study, pseudomonas aeruginosa, a plant growth promoting rhizobacterium and paecilomyces lilacinus, an egg parasite of root knot and ...201526373176
tomato i2 immune receptor can be engineered to confer partial resistance to the oomycete phytophthora infestans in addition to the fungus fusarium oxysporum.plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat (nlr)-containing proteins, to defend against invading pathogens and activate immune responses. how nlr receptors respond to pathogens is inadequately understood. we previously reported single-residue mutations that expand the response of the potato immune receptor r3a to avr3a(em), a stealthy effector from the late blight oomycete pathogen phytophthora infestans. i2, another nlr that mediates ...201526367241
antifungal activity of a synthetic cationic peptide against the plant pathogens colletotrichum graminicola and three fusarium species.a small cationic peptide (jh8944) was tested for activity against a number of pathogens of agricultural crops. jh8944 inhibited conidium growth in most of the tested plant pathogens with a dose of 50 μg/ml, although one isolate of fusarium oxysporum was inhibited at 5 μg/ml of jh8944. most conidia of fusarium graminearum were killed within 6 hours of treatment with 50 μg/ml of jh8944. germinating f. graminearum conidia required 238 μg/ml of jh8944 for 90% growth inhibition. the peptide did not c ...201526361481
expression of rice chitinase gene in genetically engineered tomato confers enhanced resistance to fusarium wilt and early blight.this is the first study reporting the evaluation of transgenic lines of tomato harboring rice chitinase (rcg3) gene for resistance to two important fungal pathogens fusarium oxysporum f. sp. lycopersici (fol) causing fusarium wilt and alternaria solani causing early blight (eb). in this study, three transgenic lines tl1, tl2 and tl3 of tomato solanum lycopersicum mill. cv. riogrande genetically engineered with rice chitinase (rcg 3) gene and their r1 progeny was tested for resistance to fol by r ...201526361473
phylogenetic diversity of human pathogenic fusarium and emergence of uncommon virulent species.fusarium species cause a broad spectrum of infections. however, little is known about the etiological agents to the species level. we identified fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.201526348828
an interspecies signaling system mediated by fusaric acid has parallel effects on antifungal metabolite production by pseudomonas protegens strain pf-5 and antibiosis of fusarium spp.pseudomonas protegens strain pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. we derived mutants of pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (dapg), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide a, and toxoflavin. these mutants were tested for inhibition of the pathogens fusarium verticil ...201526655755
illumina miseq investigations on the changes of microbial community in the fusarium oxysporum f.sp. cubense infected soil during and after reductive soil disinfestation.although reductive soil disinfestation (rsd) is increasingly used for the control of soil-borne diseases, its impact on the soil microbial community during and after rsd remains poorly understood. miseq pyrosequencing, real-time pcr and denaturing gradient gel electrophoresis were performed to investigate the changes of microbial community in the fusarium oxysporum f. sp. cubense (foc) infected soil during rsd and at the simulative banana cultivation after rsd. the results showed that rsd signif ...201526640050
fusarium oxysporum volatiles enhance plant growth via affecting auxin transport and signaling.volatile organic compounds (vocs) have well-documented roles in plant-plant communication and directing animal behavior. in this study, we examine the less understood roles of vocs in plant-fungal relationships. phylogenetically and ecologically diverse strains of fusarium oxysporum, a fungal species complex that often resides in the rhizosphere of assorted plants, produce volatile compounds that augment shoot and root growth of arabidopsis thaliana and tobacco. growth responses of a. thaliana h ...201526617587
[keratomycosis due to fusarium oxysporum treated with the combination povidone iodine eye drops and oral fluconazole].in developing countries where systemic antifungal are often unavailable, treatment of filamentous fungi infection as fusarium is sometimes very difficult to treat. we report the case of a keratomycosis due to fusarium oxysporum treated by povidone iodine eye drops and oral fluconazole. the diagnosis of abscess in the cornea was retained after ophthalmological examination for a 28-year-old man with no previous ophthalmological disease, addressed to the ophthalmological clinic at the university ho ...201526597147
roles of three fusarium oxysporum calcium ion (ca(2+)) channels in generating ca(2+) signatures and controlling growth.spatial and temporal changes of cytoplasmic calcium ions ([ca(2+)]c), caused by external stimuli, are known as the ca(2+) signature and presumably control cellular and developmental responses. multiple types of ion channels, pumps, and transporters on plasma and organellar membranes modulate influx and efflux of ca(2+) to and from the extracellular environment and internal ca(2+) stores to form ca(2+) signatures. expression of a fluorescent protein-based ca(2+) probe, cameleon yc3.60, in fusariu ...201526162966
innovative approach to the accumulation of rubrosterone by fermentation of asparagus filicinus with fusarium oxysporum.rubrosterone, possessing various remarkable bioactivities, is an insect-molting c19-steroid. however, only very small amounts are available for biological tests due to its limited content from plant sources. fungi of genus fusarium have been reported to have the ability to convert c27-steroids into c19-steroids. in this study, asparagus filicinus, containing a high content of 20-hydroxyecdysone, was utilized to accumulate rubrosterone through solid fermentation by fusarium oxysporum. the results ...201526145461
complete genome sequence of a novel dsrna mycovirus isolated from the phytopathogenic fungus fusarium oxysporum f. sp. dianthi.a novel double-stranded rna (dsrna) mycovirus, designated fusarium oxysporum f. sp. dianthi mycovirus 1 (fodv1), was isolated from a strain of the phytopathogenic fungus f. oxysporum f. sp. dianthi. the fodv1 genome had four dsrna segments, designated, from the largest to the smallest one, dsrna 1, 2 3, and 4. each one of these segments contained a single open reading frame (orf). dsrna 1 (3555 bp) and dsrna 3 (2794 bp) encoded a putative rna-dependent rna polymerase (rdrp) and a putative coat p ...201526138558
proteomic analysis of conidia germination in fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling fusarium wilt of banana.conidial germination is a crucial step of the soilborne fungus fusarium oxysporum f. sp. cubense tropical race 4 (foc tr4), a most important lethal disease of banana. in this study, a total of 3659 proteins were identified by isobaric tags for relative and absolute quantitation (itraq)-based comparative proteomic approach, of which 1009 were differentially expressed during conidial germination of the fungus at 0, 3, 7, and 11 h. functional classification and bioinformatics analysis revealed that ...201526129952
biosynthesized silver nanoparticles performing as biogenic sers-nanotags for investigation of c26 colon carcinoma cells.in this work, two classes of silver nanoparticles (agnps) were biosynthesized with the goal to assess their reliability in vitro as surface-enhanced raman scattering (sers) nanotags. mycosynthesized silver nanoparticles (magnps) and phytosynthesized silver nanoparticles (pagnps) were produced through environmentally friendly procedures by reduction of silver nitrate with fusarium oxysporum cell filtrate and azadirachta indica extract, respectively. two cell lines, namely c26 murine colon carcino ...201526123850
synthesis of novel β-keto-enol derivatives tethered pyrazole, pyridine and furan as new potential antifungal and anti-breast cancer agents.recently, a new generation of highly promising inhibitors bearing β-keto-enol functionality has emerged. reported herein is the first synthesis and use of novel designed drugs based on the β-keto-enol group embedded with heterocyclic moieties such as pyrazole, pyridine, and furan, prepared in a one-step procedure by mixed claisen condensation. all the newly synthesized compounds were characterized by ft-ir, ¹h-nmr, (13)c-nmr, esi/lc-ms, elemental analysis, and evaluated for their in vitro antipro ...201526569202
[genetic diversity analysis of fusarium oxysporum f. sp. cubense populations from china using inter-simple sequence repeats-pcr (issr-pcr) technique].we used inter-simple sequence repeats (issr) markers to reveal the genetic diversity of 95 fusarium oxysporum f. sp. cubense ( foc ) isolates from banana in china, for the rational control of the disease.201526562993
plant-microbe rhizosphere interactions mediated by rehmannia glutinosa root exudates under consecutive monoculture.under consecutive monoculture, the biomass and quality of rehmannia glutinosa declines significantly. consecutive monoculture of r. glutinosa in a four-year field trial led to significant growth inhibition. most phenolic acids in root exudates had cumulative effects over time under sterile conditions, but these effects were not observed in the rhizosphere under monoculture conditions. it suggested soil microbes might be involved in the degradation and conversion of phenolic acids from the monocu ...201526515244
fungal pathogen uses sex pheromone receptor for chemotropic sensing of host plant signals.for more than a century, fungal pathogens and symbionts have been known to orient hyphal growth towards chemical stimuli from the host plant. however, the nature of the plant signals as well as the mechanisms underlying the chemotropic response have remained elusive. here we show that directed growth of the soil-inhabiting plant pathogen fusarium oxysporum towards the roots of the host tomato (solanum lycopersicum) is triggered by the catalytic activity of secreted class iii peroxidases, a famil ...201526503056
molecular phylogeny and diversity of fusarium endophytes isolated from tomato stems.plant tissues are a known habitat for two types of fusarium species: plant pathogens and endophytes. here, we investigated the molecular phylogeny and diversity of endophytic fusaria, because endophytes are not as well studied as pathogens. a total of 543 fusarium isolates were obtained from the inside of tomato stems cultivated in soils mainly obtained from agricultural fields. we then determined partial nucleotide sequences of the translation elongation factor-1 alpha (ef-1α) genes of the isol ...201526298015
efficacy of fungal decolorization of a mixture of dyes belonging to different classes.dyes are the most difficult constituents to remove by conventional biological wastewater treatment. colored wastewater is mainly eliminated by physical and chemical procedures, which are very expensive and have drawbacks. therefore, the advantage of using biological processes, such as the biotransformation of dyes, is that they may lead to complete mineralization or formation of less toxic products. to prove the possibility of using fungal processes for decolorization and other applications, the ...201526273256
comparative proteomic analyses reveal that gnt2-mediated n-glycosylation affects cell wall glycans and protein content in fusarium oxysporum.protein n-glycosylation is a ubiquitous post-translational modification that contributes to appropriate protein folding, stability, functionality and localization. n-glycosylation has been identified as an important process for morphogenesis and virulence in several fungal pathogens including fusarium oxysporum. here we conducted comparative chemical and proteome-based analyses to better understand the physiological changes associated with protein hypo-n-glycosylation in f. oxysporum n-glycosylt ...201526254006
effect of vinegar residue compost amendments on cucumber growth and fusarium wilt.fusarium wilt of cucumber caused by fusarium oxysporum f. sp. cucumerinum j. h. owen is one of the major destructive soilborne diseases and results in considerable yield losses. methyl bromide was once the most effective disease control method but has been confirmed as harmful to the environment. using suppressive media as biological controls to assist crop growth is becoming popular. in this study, fusarium wilt of cucumber was successfully controlled by a newly identified suppressive media: vi ...201526250808
salecan enhances the activities of β-1,3-glucanase and decreases the biomass of soil-borne fungi.salecan, a linear extracellular polysaccharide consisting of β-1,3-d-glucan, has potential applications in the food, pharmaceutical and cosmetic industries. the objective of this study was to evaluate the effects of salecan on soil microbial communities in a vegetable patch. compositional shifts in the genetic structure of indigenous soil bacterial and fungal communities were monitored using culture-dependent dilution plating, culture-independent pcr-denaturing gradient gel electrophoresis (dgge ...201526247592
chemotropic sensing in fungus-plant interactions.growth of fungal hyphae is guided by a variety of chemical gradients, including nutrients, mating pheromones or host compounds. over 100 years after chemotropism was first reported in fungus-plant interactions, our knowledge on the host signals, fungal receptors and cellular pathways is still rudimentary. genetic analysis in the model organisms saccharomyces cerevisiae and neurospora crassa has provided valuable insights into the chemotropic machinery of the fungal hypha. moreover, recent studie ...201526247120
manipulating the banana rhizosphere microbiome for biological control of panama disease.panama disease caused by fusarium oxysporum f. sp. cubense infection on banana is devastating banana plantations worldwide. biological control has been proposed to suppress panama disease, though the stability and survival of bio-control microorganisms in field setting is largely unknown. in order to develop a bio-control strategy for this disease, 16s rrna gene sequencing was used to assess the microbial community of a disease-suppressive soil. bacillus was identified as the dominant bacterial ...201526242751
genetic analysis of verticillium wilt resistance in a backcross inbred line population and a meta-analysis of quantitative trait loci for disease resistance in cotton.verticillium wilt (vw) and fusarium wilt (fw), caused by the soil-borne fungi verticillium dahliae and fusarium oxysporum f. sp. vasinfectum, respectively, are two most destructive diseases in cotton production worldwide. root-knot nematodes (meloidogyne incognita, rkn) and reniform nematodes (rotylenchulus reniformis, rn) cause the highest yield loss in the u.s. planting disease resistant cultivars is the most cost effective control method. numerous studies have reported mapping of quantitative ...201526239843
compost and biochar alter mycorrhization, tomato root exudation, and development of fusarium oxysporum f. sp. lycopersici.soil amendments like compost and biochar are known to affect soil properties, plant growth as well as soil borne plant pathogens. complex interactions based on microbial activity and abiotic characteristics are supposed to be responsible for suppressive properties of certain substrates, however, the specific mechanisms of action are still widely unknown. in the present study, the main focus was on the development of the soil borne pathogen, fusarium oxysporum f.sp. lycopersici (fol) in tomato (s ...201526217373
first report on fusarium wilt of zucchini caused by fusarium oxysporum, in korea.fusarium wilt of zucchini in jeonju, korea, was first noticed in may 2013. symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. infected plants eventually died during growth. based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rdna and translation elongation factor 1α), the fungus was identified as fusarium oxysporum. pathogenicity of a representative isolate was demonstrated via ...201526190927
fusarium wilt of banana.banana (musa spp.) is one of the world's most important fruits. in 2011, 145 million metric tons, worth an estimated $44 billion, were produced in over 130 countries. fusarium wilt (also known as panama disease) is one of the most destructive diseases of this crop. it devastated the 'gros michel'-based export trades before the mid-1900s, and threatens the cavendish cultivars that were used to replace it; in total, the latter cultivars are now responsible for approximately 45% of all production. ...201526057187
development of co-dominant scar markers linked to resistant gene against the fusarium oxysporum f. sp. radicis-lycopersici.we developed highly reliable co-dominant scar markers linked to the frl gene. forl testing is difficult. the marker is expected to be quickly adapted for mas by tomato breeders. fusarium oxysporum f. sp. radicis-lycopersici causes fusarium crown and root rot (fcr), an economically important soil-borne disease of tomato. the resistance against fcr is conferred by a single dominant gene (frl) located on chromosome 9. the aim of this study was to develop molecular markers linked to the frl gene for ...201526037087
focvel1 influences asexual production, filamentous growth, biofilm formation, and virulence in fusarium oxysporum f. sp. cucumerinum.velvet genes play critical roles in the regulation of diverse cellular processes. in current study, we identified the gene focvel1, a homolog of fusarium graminearum vela, in the plant pathogenic fungus f. oxysporum f. sp. cucumerinum. this pathogen causes the destructive disease called cucumber fusarium wilt (cfw), which severely affects the production and marketing of this vegetable worldwide. transcript analyses revealed high expression of focvel1 during conidiophore development. disruption o ...201525999976
genome-wide analysis in three fusarium pathogens identifies rapidly evolving chromosomes and genes associated with pathogenicity.pathogens and hosts are in an ongoing arms race and genes involved in host-pathogen interactions are likely to undergo diversifying selection. fusarium plant pathogens have evolved diverse infection strategies, but how they interact with their hosts in the biotrophic infection stage remains puzzling. to address this, we analyzed the genomes of three fusarium plant pathogens for genes that are under diversifying selection. we found a two-speed genome structure both on the chromosome and gene grou ...201525994930
emergence of fusarioses in a university hospital in turkey during a 20-year period.fusarium species have started appearing increasingly as the main cause of infections, particularly in immunocompromised patients. in this study, we aimed to present the first epidemiological data from turkey, analyze fusariosis cases that have been monitored in a university hospital during the past 20 years, identify the responsible fusarium species, and determine antifungal susceptibilities. a total of 47 cases of fusariosis was included in the study. fusarium isolates were identified by multil ...201525994844
in vitro study of the growth, development and pathogenicity responses of fusarium oxysporum to phthalic acid, an autotoxin from lanzhou lily.continuous monoculture of lanzhou lily (lilium davidii var. unicolor cotton) results in frequent incidence of fusarium wilt caused by fusarium oxysporum. phthalic acid (pa), a principal autotoxin from root exudates of lanzhou lily, is involved in soil sickness by inducing autotoxicity. the aim of this study was to evaluate the direct allelopathic effects of pa on the growth, development and pathogenicity of f. oxysporum in vitro based on an ecologically relevant soil concentration. the results s ...201525994089
cutin from watermelon peels: a novel inducer for cutinase production and its physicochemical characterization.cutin a polymeric biological macromolecule made up of esterified polyacids which acts as an inducer of cutinase. the present work was based on screening and characterization of newer cutin source from the agro industrial wastes as inducers of cutinase production by fusarium oxysporum mtcc 2480. cutin isolated from peels of multi green colored watermelon (wmc) yielded 6.77 u/ml as compared to that 9.64 u/ml of cutinase using apple cutin. the ftir and (13)c cp-mas solid state nmr studies indicated ...201525987459
synthesis of a novel series of fluoroarene derivatives of artemisinin as potent antifungal and anticancer agent.a series of new fluoroarene derivatives of artemisinin were prepared using suzuki and sonogashira cross-coupling reactions. an antifungal and antitumor activity was evaluated against opportunistic pathogen fusarium oxysporum and hela cancer cell line. all these derivatives have shown moderate to good activity.201526099535
use of fusarium oxysporum schl cucumerinum transformed with green fluorescent protein to follow infection patterns in three cucurbit plants.fusarium oxysporum schl is a soil-borne pathogen fungus, which could cause plant wilt diseases and caused great economic losses of agricultural production all over the world. however, the species of melon wilt diseases are too complex to breed stable disease-resistant melon strain. therefore, exploring the main specialized differentiation factors of cucurbit wilt pathogen is important for elucidating the pathogenesis. in this study, we constructed green fluorescent protein (gfp)-labeled fusarium ...201527617956
characterization of vacuolar amino acid transporter from fusarium oxysporum in saccharomyces cerevisiae.fusarium oxysporum causes wilt disease in many plant families, and many genes are involved in its development or growth in host plants. a recent study revealed that vacuolar amino acid transporters play an important role in spore formation in schizosaccharomyces pombe and saccharomyces cerevisiae. to investigate the role of vacuolar amino acid transporters of this phytopathogenic fungus, the foxg_11334 (foavt3) gene from f. oxysporum was isolated and its function was characterized. transcription ...201526083447
[inhibition of amaranthus retroflexus by wasp gut fungal isolate fusarium oxysporum mf06].to study the inhibitory effect of wasp gut fungus against the radicle growth of amaranthus retroflexus for the development of herbicides from microorganisms.201526065271
effects of volatile organic compounds from streptomyces albulus njzjsa2 on growth of two fungal pathogens.a streptomyces albulus strain njzjsa2 was isolated from the forest soil sample of tzu-chin mountain (nanjing china) and identified based on its morphological and physiological properties and 16s rdna gene sequence analysis. the strain s. albulus njzjsa2 was evaluated for the production of antifungal volatile organic compounds (vocs) against two fungal pathogens. results showed that the vocs generated by s. albulus njzjsa2 inhibited mycelial growth of sclerotinia sclerotiorum (ss) and fusarium ox ...201526059065
fungal cell wall polymer based nanoparticles in protection of tomato plants from wilt disease caused by fusarium oxysporum f.sp. lycopersici.cell wall polymer (chitosan) was isolated from fusarium oxysporum f.sp. lycopersici. they were cross linked with sodium tripolyphosphate (tpp) to synthesize nanoparticles (cwp-np). the nanoparticles were characterized by ftir, dls, sem, xrd and nmr analyses. the isolated cwp-np exhibit antifungal activity under in vitro condition. the foliar application of the cwp-np to tomato plants challenged with f. oxysporum f. sp. lycopersici showed delay in wilt disease symptom expression and reduce the wi ...201526344296
comparative proteomics analyses of two races of fusarium oxysporum f. sp. conglutinans that differ in pathogenicity.fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in a variety of plants. f. oxysporum f. sp. conglutinans (foc), which comprises two races, can cause wilt disease in cabbage. compared with race 1 (52557(-tm), r1), race 2 (58385(-tm), r2) exhibits much stronger pathogenicity. here, we provide the first proteome reference maps for foc mycelium and conidia and identify 145 proteins with different abundances among the two races. of these proteins, most of the hi ...201526333982
pharmacogenomic and molecular docking studies on the cytotoxicity of the natural steroid wortmannin against multidrug-resistant tumor cells.wortmannin is a cytotoxic compound derived from the endophytic fungi fusarium oxysporum, penicillium wortmannii and penicillium funiculosum that occurs in many plants, including medicinal herbs. the rationale to develop novel anticancer drugs is the frequent development of tumor resistance to the existing antineoplasic agents. therefore, it is mandatory to analyze resistance mechanisms of novel drug candidates such as wortmannin as well to bring effective drugs into the clinic that have the pote ...201525636880
correction: genome and transcriptome analysis of the fungal pathogen fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. 201525629969
preparation and characterization of a novel extracellular polysaccharide with antioxidant activity, from the mangrove-associated fungus fusarium oxysporum.marine fungi are recognized as an abundant source of extracellular polysaccharides with novel structures. mangrove fungi constitute the second largest ecological group of the marine fungi, and many of them are new or inadequately described species and may produce extracellular polysaccharides with novel functions and structures that could be explored as a source of useful polymers. the mangrove-associated fungus fusarium oxysporum produces an extracellular polysaccharide, fw-1, when grown in pot ...201525627692
fubt, a putative mfs transporter, promotes secretion of fusaric acid in the cotton pathogen fusarium oxysporum f. sp. vasinfectum.fusaric acid (fa) is a key component in virulence and symptom development in cotton during infection by fusarium oxysporum. a putative major facilitator superfamily (mfs) transporter gene was identified downstream of the polyketide synthase gene responsible for the biosynthesis of fa in a region previously believed to be unrelated to the known fa gene cluster. disruption of the transporter gene, designated fubt, resulted in loss of fa secretion, decrease in fa production and a decrease in resist ...201525627440
a novel ionic liquid-tolerant fusarium oxysporum bn secreting ionic liquid-stable cellulase: consolidated bioprocessing of pretreated lignocellulose containing residual ionic liquid.in this study, microbial communities from chemicals polluted microhabitats were cultured with the addition of imidazolium-based ionic liquid (il) to enrich for il-tolerant microbes. a strain of fusarium oxysporum bn producing cellulase from these enrichments was capable of growing in 10% (w/v) 1-ethyl-3-methylimidazolium phosphinate, much higher than the normal il concentrations in the lignocellulose regenerated from ils. cellulase secreted by the strain showed high resistance to ils based on ph ...201525625459
activity of haliscosamine against fusarium oxysporum f.sp. melonis: in vitro and in vivo analysis.marine sponges are a potential source of new molecules with diverse biological activities. we have previously isolated a sphingosine derivative, (9z)-2-amino-docos-9-ene-1,3,13,14-tetraol (haliscosamine) from the moroccan sea sponge haliclona viscosa. the aim of this study was to test haliscosamine in vitro and in vivo for its antifungal activity against fusarium oxysporum f.sp. melonis causing fusarium wilt of melon. overall, in vitro test showed that haliscosamine has a similar effect as desog ...201525625038
characterization and stress-induced expression analysis of alfin-like transcription factors in brassica rapa.the alfin-like (al) transcription factors (tfs) family is involved in many developmental processes, including the growth and development of roots, root hair elongation, meristem development, etc. however, stress resistance-related function and the regulatory mechanism of these tfs have yet to be elucidated. this study identified 15 brassica rapa al (bral) tfs from brad database, analyzed the sequences and profiled their expression first time in response to fusarium oxysporum f. sp. conglutinans ...201525618423
synthesis and in vitro antifungal efficacy of cu-chitosan nanoparticles against pathogenic fungi of tomato.cu-chitosan nanoparticles were synthesized and evaluated for their growth promotory and antifungal efficacy in tomato (solanum lycopersicum mill). physico-chemical characterization of the developed cu-chitosan nanoparticles was carried out by dls, ftir, tem, sem-eds and aas. the study highlighted the stability and porous nature of cu-chitosan nanoparticles. laboratory synthesized nanoparticles showed substantial growth promotory effect on tomato seed germination, seedling length, fresh and dry w ...201525617841
water balance altered in cucumber plants infected with fusarium oxysporum f. sp. cucumerinum.fusarium wilt is caused by the infection and growth of the fungus fusarium oxysporum in the xylem of host plants. the physiological responses of cucumbers that are infected with fusarium oxysporum f. sp. cucumerinum (foc) was studied in pot and hydroponic experiments in a greenhouse. the results showed that although water absorption and stem hydraulic conductance decreased markedly in infected plants, large amounts of red ink accumulated in the leaves of infected cucumber plants. the transpirati ...201525579504
autophagy contributes to regulation of nuclear dynamics during vegetative growth and hyphal fusion in fusarium oxysporum.in the fungal pathogen fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. here we examined the role of autophagy in fusion-induced nuclear degradation. a search of the f. oxysporum genome database for autophagy pathway components identified putative orthologs of 16 core autophagy-related (atg) genes in yeast, including the ubiquitin-like protein at ...201525560310
[repeating the platelia™ aspergillus test in samples positive for serum galactomannan: is it necessary?].the manufacturers of the platelia™ aspergillus test recommend retesting every sample with a positive result to galactomannan detection.201525556050
effect of biosolarisation on the microbial populations of substrates infested with fusarium oxysporum by pcr-dgge.biosolarisation consists of combining solarisation and organic matter application for controlling soilborne pathogens. the effects of this control strategy on the microbial community is almost unknown and needs to be investigated with molecular tools. the aim of the research was to investigate how biosolarisation can affect the structure of the microbial populations evaluated by a culture independent method using dgge of pcr-amplified 18s-its genes-coding fragments from dna extracted directly fr ...201527141761
characterization and biocontrol potent of streptomyces sp. isolated from the rhizosphere of ononis angustissima lam.a total of 40 actinomycetes isolated from rhizosphere soils of ononis angustissima lam. were in vitro tested for their antagonism against deferent pathogenic microorganisms by streak assay. among the isolates, four (21, 2a26, 1b10 and 2c34) present a potent antagonism against both pathogenic bacteria and fungi, they were selected, identified by 16s rdna sequence analysis and phenotypic properties, and tested for their antimicrobial activity as well as their biocontrol potential against chickpea ...201527141751
pre-planting treatments with phosphite-based products against different foliar and soil-borne pathogens of vegetable crops.fifteen experimental trials were carried out under greenhouse conditions to evaluate the efficacy of preventative treatments based on phosphite salts on the following pathosystems: tomato/phytophthora nicotianae, zucchini/p. capsici, lettuce/fusarium oxysporum f.sp. iactucae, rocket/fusarium oxysporum f. sp. raphani, wild rocket/plectosphaerella cucumerina and basii/peronospora belbahrii. the possible use of phosphite salts in nursery cultivation systems is considered in comparison with chemical ...201527141741
biochemical defenses induced by mycorrhizae fungi glomus mosseae in controlling strawberry fusarium wilt.the effect of vam on reducing wilt caused by fusarium oxysporum schlecht. f.sp. fragariae winks et williams (fo) infection in strawberry and the possible mechanisms involved were investigated. two key substance involved in disease defenses, lignin and hydroxyproline-rich glycoprotein were induced and formed in the cell wall of strawberry root, and the peak content of lignin and hydroxyproline-rich glycoprotein occurred on the 25(th) day (149.52mg/g) and on the 15(th) day (10.08 mg/g). the activi ...201526998177
the application of arabidopsis thaliana in studying tripartite interactions among plants, beneficial fungal endophytes and biotrophic plant-parasitic nematodes.the research demonstrated that arabidopsis can be used as a model system for studying plant-nematode-endophyte tripartite interactions; thus, opening new possibilities for further characterizing the molecular mechanisms behind these interactions. arabidopsis has been established as an important model system for studying plant biology and plant-microbe interactions. we show that this plant can also be used for studying the tripartite interactions among plants, the root-knot nematode meloidogyne i ...201525548000
comparative study of the bioconversion process using r-(+)- and s-(-)-limonene as substrates for fusarium oxysporum 152b.this study compared the bioconversion process of s-(-)-limonene into limonene-1,2-diol with the already established biotransformation of r-(+)-limonene into α-terpineol using the same biocatalyst in both processes, fusarium oxysporum 152b. the bioconversion of the s-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. when submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limone ...201525529726
[cloning and functional characterization of pathogenesis-related pr10-1 gene in panax notoginseng].with homology cloning approaches coupling with race (rapid-amplification of cdna ends) techniques, the full-length coding sequence of pathogenesis-related protein pr10-1 with differential expression was cloned from the total rna of the root of panax notoginseng, and its function was explored furtherly. as a result, the longest 465 bp orf (named as pnpr10-1 with the accession no. kj741402 in genbank) was detected from the cloned sequence with full-length of cdna of 863 bp. the corresponding pepti ...201525975034
effects of water regime, crop residues, and application rates on control of fusarium oxysporum f. sp. cubense.biological soil disinfestation is an effective method to control soil-borne disease by flooding and incorporating with organic amendments, but field conditions and resources sometimes limited its practical application. a laboratory experiment was conducted to develop practice guidelines on controlling fusarium wilt, a widespread banana disease caused by fusarium oxysporum f. sp. cubense (foc). foc infested soil incorporated with rice or maize straw at rates of 1.5 tons/ha and 3.0 tons/ha was inc ...201525968255
the avr2-six5 gene pair is required to activate i-2-mediated immunity in tomato.plant-invading microbes betray their presence to a plant by exposure of antigenic molecules such as small, secreted proteins called 'effectors'. in fusarium oxysporum f. sp. lycopersici (fol) we identified a pair of effector gene candidates, avr2-six5, whose expression is controlled by a shared promoter. the pathogenicity of avr2 and six5 fol knockouts was assessed on susceptible and resistant tomato (solanum lycopersicum) plants carrying i-2. the i-2 nb-lrr protein confers resistance to fol rac ...201525967461
isolation of a hemagglutinin with potent antiproliferative activity and a large antifungal defensin from phaseolus vulgaris cv. hokkaido large pinto beans.lectins (hemagglutinins) are defined as sugar-binding proteins or glycoproteins with various biological activities. a 60 kda dimeric hemagglutinin with a blocked n-terminus was isolated in large yield (190 mg/60 g) from the common edible bean phaseolus vulgaris cv. hokkaido large pinto bean. its hemagglutinating, antifungal, and antitumor activities as well as the effects of carbohydrate and metal ions on its hemagglutinating activity were examined. it inhibited the proliferation of nasopharynge ...201525965006
demethylation of arsenic limits its volatilization in fungi.arsenic (as) biomethylation is increasingly being regarded as a promising method to volatize as from the environment; however, the as volatilization efficiency of most microorganisms is low. here, the speciation transformation of dimethylarsinic acid (dma) as an important methylation intermediate in the cells of fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1 were investigated. these fungal strains have been certified to volatilize as from as-loade ...201525951513
effect of eugenol against streptococcus agalactiae and synergistic interaction with biologically produced silver nanoparticles.streptococcus agalactiae (group b streptococci (gbs)) is an important infections agent in newborns associated with maternal vaginal colonization. intrapartum antibiotic prophylaxis in gbs-colonized pregnant women has led to a significant reduction in the incidence of early neonatal infection in various geographic regions. however, this strategy may lead to resistance selecting among gbs, indicating the need for new alternatives to prevent bacterial transmission and even to treat gbs infections. ...201525945115
disseminated fusariosis and hematologic malignancies, a still devastating association. report of three new cases.fungi of the genus fusarium are primarily plant pathogens and saprobes that produce disseminated infections in immunologically deficient humans. after aspergillosis, disseminated fusariosis is the second most common cause of invasive infection by filamentous fungi in patients with hematologic malignancies or those undergoing transplants of hematopoietic progenitors.201525936697
metabolic profiling of chickpea-fusarium interaction identifies differential modulation of disease resistance pathways.chickpea is the third most widely grown legume in the world and mainly used as a vegetarian source of human dietary protein. fusarium wilt, caused by fusarium oxysporum f. sp. ciceri (foc), is one of the major threats to global chickpea production. host resistance is the best way to protect crops from diseases; however, in spite of using various approaches, the mechanism of foc resistance in chickpea remains largely obscure. in the present study, non-targeted metabolic profiling at several time ...201525935544
analyses of fusarium wilt race 3 resistance in upland cotton (gossypium hirsutum l.).fusarium wilt [fusarium oxysporum f.sp. vasinfectum (fov) atk. sny & hans] represents a serious threat to cotton (gossypium spp.) production. for the last few decades, the fov pathogen has become a significant problem in uzbekistan causing severe wilt disease and yield losses of g. hirsutum l. cultivars. we present the first genetic analyses of fov race 3 resistance on uzbek cotton germplasm with a series of field and greenhouse artificial inoculation-evaluations and inheritance studies. the fie ...201525896369
synthesis, insecticidal, and fungicidal screening of some new synthetic quinoline derivatives.this paper describes the synthesis of a series of quinolines graphted with hydrazones, pyrazoles, pyridazine, phthalazine, triazepinone, semicarbazide, and thiomorpholide moieties and four metal complexes. these derivatives were screened against fusarium oxysporum and the red palm weevil (rpw) rhynchophorus ferrugineus oliver (coleopteran: curculionidae) as palm pathogens. only chlorinated quinolines were active against these organisms with hydrazones being good fungicides, while those modified ...201525895344
n2o production, a widespread trait in fungi.n2o is a powerful greenhouse gas contributing both to global warming and ozone depletion. while fungi have been identified as a putative source of n2o, little is known about their production of this greenhouse gas. here we investigated the n2o-producing ability of a collection of 207 fungal isolates. seventy strains producing n2o in pure culture were identified. they were mostly species from the order hypocreales order-particularly fusarium oxysporum and trichoderma spp.-and to a lesser extent s ...201525894103
ethanol effect on metabolic activity of the ethalogenic fungus fusarium oxysporum.fusarium oxysporum is a filamentous fungus which has attracted a lot of scientific interest not only due to its ability to produce a variety of lignocellulolytic enzymes, but also because it is able to ferment both hexoses and pentoses to ethanol. although this fungus has been studied a lot as a cell factory, regarding applications for the production of bioethanol and other high added value products, no systematic study has been performed concerning its ethanol tolerance levels.201525887038
development of loop-mediated isothermal amplification (lamp) assay for rapid detection of fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpea.fusarium oxysporum f. sp. ciceris (foc), the causal agent of fusarium wilt is a devastating pathogen of chickpea. in chickpea, various soil borne pathogens produce (s) similar symptoms, therefore cannot be distinguished easily at field level. there is real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of fusarium wilt outbreaks.201525886622
gene expression patterns and dynamics of the colonization of common bean (phaseolus vulgaris l.) by highly virulent and weakly virulent strains of fusarium oxysporum.the dynamics of root and hypocotyl colonization, and the gene expression patterns of several fungal virulence factors and plant defense factors have been analyzed and compared in the interaction of two fusarium oxysporum f. sp. phaseoli strains displaying clear differences in virulence, with a susceptible common bean cultivar. the growth of the two strains on the root surface and the colonization of the root was quantitatively similar although the highly virulent (hv) strain was more efficient r ...201525883592
fungal cytochrome p450 monooxygenases of fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered saccharomyces cerevisiae.omega hydroxy fatty acids (ω-ohfas) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. however, the terminal oxygenation of saturated or unsaturated fatty acids for the synthesis of ω-ohfas is intricate to accomplish through chemocatalysis, due to the selectivity and controlled reactivity in c-h oxygenation reactions. cytochrome p450, the ubiquitous enzyme is capable of catalyzing the select ...201525880760
priming of seeds with methyl jasmonate induced resistance to hemi-biotroph fusarium oxysporum f.sp. lycopersici in tomato via 12-oxo-phytodienoic acid, salicylic acid, and flavonol accumulation.methyl jasmonate (meja) was tested by seed treatment for its ability to protect tomato seedlings against fusarium wilt caused by the soil-borne fungal pathogen fusarium oxysporum f.sp. lycopersici. isolated from solanum lycopersicon l. seeds, cv. beta fungus was identified as f. oxysporum f.sp. lycopersici race 3 fungus by using phytopathological and molecular methods. meja applied at 0.01, 0.1 and 1 mm reduced spore germination and mycelial growth in vitro. soaking of tomato seeds in meja solut ...201525867625
glycogen catabolism, but not its biosynthesis, affects virulence of fusarium oxysporum on the plant host.the role of glycogen metabolism was investigated in the fungal pathogen fusarium oxysporum. targeted inactivation was performed of genes responsible for glycogen biosynthesis: gnn1 encoding glycogenin, gls1 encoding glycogen synthase, and gbe1 encoding glycogen branching enzyme. moreover genes involved in glycogen catabolism were deleted: gph1 encoding glycogen phosphorylase and gdb1 encoding glycogen de-branching enzyme. glycogen reserves increased steadily during growth of the wild type strain ...201525865793
mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in fusarium oxysporum f. sp. cubense.fusarium oxysporum f. sp. cubense (foc) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. however, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. in this study, we showed that null mutation of three mitogen-activated protein (map) kinase genes, designated as foslt2, fomkk2 an ...201525849862
[differential gene expression in incompatible interaction between lilium regale wilson and fusarium oxysporum f. sp. lilii revealed by combined ssh and microarray analysis].fusarium wilt, caused by a soilborne pathogen fusarium oxysporum f. sp. lilii, is the major disease of lily (lilium l.). in order to isolate the genes differentially expressed in a resistant reaction to f. oxysporum in l. regale wilson, a cdna library was constructed with l. regale root during f. oxysporum infection using the suppression subtractive hybridization (ssh), and a total of 585 unique expressed sequence tags (ests) were obtained. furthermore, the gene expression profiles in the incomp ...201525845232
[allelopathic effects of phenolic compounds of melon root exudates on fusarium oxysporum f. sp. melonis].in this study, the phenolic compounds of melon root exudates were identified by hplc and seven phenolic compounds including gallic acid, phthalic acid, syringic acid, salicylic acid, ferulic acid, benzoic acid and cinnamic acid were observed. the laboratory experiment showed that ferulic acid, benzoic acid and cinnamic acid of 0.1 and 0.25 mmol x l(-1) could significantly promote the germination of fusarium oxysporum f. sp. melonis spore while salicylic acid inhibited the spore germination to so ...201425509089
characterization of anticancer, dnase and antifungal activity of pumpkin 2s albumin.the plant 2s albumins exhibit a spectrum of biotechnologically exploitable functions. among them, pumpkin 2s albumin has been shown to possess rnase and cell-free translational inhibitory activities. the present study investigated the anticancer, dnase and antifungal activities of pumpkin 2s albumin. the protein exhibited a strong anticancer activity toward breast cancer (mcf-7), ovarian teratocarcinoma (pa-1), prostate cancer (pc-3 and du-145) and hepatocellular carcinoma (hepg2) cell lines. ac ...201424814706
analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic fusarium oxysporum cs-20.a defence response can be induced by nonpathogenic fusarium oxysporum cs-20 in several crops, but the molecular mechanism has not been clearly demonstrated. in the present study, we analysed the defence mechanism of a susceptible cucumber cultivar (cucumis sativus l. 9930) against a pathogen (f. oxysporum f. sp. cucumerinum) through the root precolonization of cs-20. a challenge inoculation assay indicated that the disease severity index (dsi) was reduced, ranging from 18.83 to 61.67 in comparis ...201424810367
retransformation of marker-free potato for enhanced resistance against fungal pathogens by pyramiding chitinase and wasabi defensin genes.multi-auto-transformation vector system has been one of the strategies to produce marker-free transgenic plants without using selective chemicals and plant growth regulators and thus facilitating transgene stacking. in the study reported here, retransformation was carried out in marker-free transgenic potato cv. may queen containing chic gene (isolated from streptomyces griseus strain hut 6037) with wasabi defensin (wd) gene (isolated from wasabia japonica) to pyramid the two disease resistant g ...201424802621
[features of interaction bacterial strains micrococcus luteus lbk1 from plants varieties/hybrids cucumber and sweet pepper and with fungus fusarium oxysporum scelecht].the article presents the results of studying the impact of bacterial strain m. luteus lbk1, stimulating the growth and development of plant varieties/hybrids of cucumber and sweet pepper on the intensity of sporulation of the fungus f. oxysporum scelecht--fusariose rot pathogen.201424800513
development of dart markers and assessment of diversity in fusarium oxysporum f. sp. ciceris, wilt pathogen of chickpea (cicer arietinum l.).fusarium oxysporum f. sp. ciceris (foc), the causal agent of fusarium wilt of chickpea is highly variable and frequent recurrence of virulent forms have affected chickpea production and exhausted valuable genetic resources. the severity and yield losses of fusarium wilt differ from place to place owing to existence of physiological races among isolates. diversity study of fungal population associated with a disease plays a major role in understanding and devising better disease control strategie ...201424912854
non-thermal plasma treatment diminishes fungal viability and up-regulates resistance genes in a plant host.reactive oxygen and nitrogen species can have either harmful or beneficial effects on biological systems depending on the dose administered and the species of organism exposed, suggesting that application of reactive species can possibly produce contradictory effects in disease control, pathogen inactivation and activation of host resistance. a novel technology known as atmospheric-pressure non-thermal plasma represents a means of generating various reactive species that adversely affect pathoge ...201424911947
the tomato wilt fungus fusarium oxysporum f. sp. lycopersici shares common ancestors with nonpathogenic f. oxysporum isolated from wild tomatoes in the peruvian andes.fusarium oxysporum is an ascomycetous fungus that is well-known as a soilborne plant pathogen. in addition, a large population of nonpathogenic f. oxysporum (npf) inhabits various environmental niches, including the phytosphere. to obtain an insight into the origin of plant pathogenic f. oxysporum, we focused on the tomato (solanum lycopersicum) and its pathogenic f. oxysporum f. sp. lycopersici (fol). we collected f. oxysporum from wild and transition solanum spp. and modern cultivars of tomato ...201424909710
differential protein accumulations in isolates of the strawberry wilt pathogen fusarium oxysporum f. sp. fragariae differing in virulence.this study was conducted to define differences in fusarium oxysporum f. sp. fragariae (fof) isolates with different virulence efficiency to strawberry at the proteome level, in combination with their differences in mycelial growth, conidial production and germination. comparative proteome analyses revealed substantial differences in mycelial proteomes between fof isolates, where the 54 differentially accumulated protein spots were consistently over-accumulated or exclusively in the highly virule ...201424907490
quantum dot and superparamagnetic nanoparticle interaction with pathogenic fungi: internalization and toxicity profile.for several years now, nanoscaled materials have been implemented in biotechnological applications related to animal (in particular human) cells and related pathologies. however, the use of nanomaterials in plant biology is far less widespread, although their application in this field could lead to the future development of plant biotechnology applications. for any practical use, it is crucial to elucidate the relationship between the nanomaterials and the target cells. in this work we have eval ...201424853082
neuroprotective effects of bikaverin on h2o2-induced oxidative stress mediated neuronal damage in sh-sy5y cell line.the generation of free radicals and oxidative stress has been linked to several neurodegenerative diseases including parkinson's disease, alzheimer's disease, huntington's disease, and amyotrophic lateral sclerosis. the use of free radical scavenging molecules for the reduction of intracellular reactive oxygen species is one of the strategies used in the clinical management of neurodegeneration. fungal secondary metabolism is a rich source of novel molecules with potential bioactivity. in the cu ...201424848007
isolation, structure determination, and antiaging effects of 2,3-pentanediol from endophytic fungus of curcuma amada and docking studies.an endophytic fungus was isolated from the rhizomes of curcuma amada (zingiberaceae), which was identified as fusarium oxysporum on the basis of its morphological and molecular characters. chromatographic separation and spectroscopic analysis of the fungal metabolite (chloroform extract) led to the identification of one pure compound having molecular formula c5h12o2, i.e., 2,3-pentanediol (1). activity analysis of compound 1 demonstrated improved antiaging (antioxidant, thermotolerance) properti ...201424515569
functional analysis of chimeric lysin motif domain receptors mediating nod factor-induced defense signaling in arabidopsis thaliana and chitin-induced nodulation signaling in lotus japonicus.the expression of chimeric receptors in plants is a way to activate specific signaling pathways by corresponding signal molecules. defense signaling induced by chitin from pathogens and nodulation signaling of legumes induced by rhizobial nod factors (nfs) depend on receptors with extracellular lysin motif (lysm) domains. here, we constructed chimeras by replacing the ectodomain of chitin elicitor receptor kinase 1 (atcerk1) of arabidopsis thaliana with ectodomains of nf receptors of lotus japon ...201424506212
induced defense-related proteins in soybean (glycine max l. merrill) plants by carnobacterium sp. sj-5 upon challenge inoculation of fusarium oxysporum.the aim of the present study was to analyze induced expression of defense-related proteins in the soybean plants by rhizobacterial stain carnobacterium sp. sj-5 upon challenge inoculation with fusarium oxysporum. determination of the enzymatic activity of the different defense-related enzymes, phenylalanine ammonia lyase (pal), lipoxygenase (lox), peroxidase (pod) and polyphenol oxidase (ppo) was performed in the major parts of glycine max l. merrill using spectrophotometric method. native-polya ...201424504695
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