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pathogenic and genetic variation in the japanese strains of fusarium oxysporum f. sp. melonis.abstract pathogenic variation among 41 japanese strains of fusarium oxysporum f. sp. melonis was analyzed by pathogenicity tests with muskmelon, oriental melon, and oriental pickling melon cultivars. based on pathogenicity to muskmelon cvs. amus and ohi and oriental melon cv. ogon 9, 41 strains were divided into 3 groups that corresponded completely to risser's races 0, 2, and 1,2y. to further characterize pathogenic variation within the forma specialis and races, strains were assayed for pathog ...199818944886
interactions of pratylenchus thornei and fusarium oxysporum f. sp. ciceris on chickpea.abstract fusarium oxysporum f. sp. ciceris and the root-lesion nematode pratylenchus thornei coinfect chickpeas in southern spain. the influence of root infection by p. thornei on the reaction of fusarium wilt-susceptible (cps 1 and pv 61) and wilt-resistant (uc 27) chickpea cultivars to f. oxysporum f. sp. ciceris race 5 was investigated under controlled and field conditions. severity of fusarium wilt was not modified by coinfection of chickpeas by p. thornei and f. oxysporum f. sp. ciceris, in ...199818944890
effect of relative humidity on sporulation of fusarium oxysporum in various formulations and effect of water on spore movement through soil.abstract fusarium oxysporum f. sp. erythroxyli is being investigated as a mycoherbicide for the narcotic plant coca. sporulation of the fungus in seven formulations containing different organic substrates and movement of its propagules through soil were studied. the formulations were a granular wheat flour/kaolin (pesta); an extruded wheat and rice flour (c-6); and five alginate pellet products containing corn cobs, soybean hull fiber, canola meal, rice flour, or rice flour plus canola oil. form ...199818944907
dispersal of formulations of fusarium oxysporum f. sp. erythroxyli and f. oxysporum f. sp. melonis by ants.abstract a natural epidemic of fusarium wilt on coca (erythroxylum coca) in peru prompted the suggestion of possibly using the pathogen fusarium oxysporum f. sp. erythroxyli as a mycoherbicide against this narcotic plant. during field trials conducted in kauai, hi, to test the pathogenicity of the coca wilt pathogen, ants were observed removing formulations from test plots. while removal of formulations by ants was considered detrimental with respect to conducting field tests, ant removal was co ...199818944963
systematic numbering of vegetative compatibility groups in the plant pathogenic fungus fusarium oxysporum. 199818944995
peanut-cotton-rye rotations and soil chemical treatment for managing nematodes and thrips.in the southeastern united states, a cotton-peanut rotation is attractive because of the high value and extensive planting of both crops in the region. the objective of this experiment was to determine the effects of cotton-peanut rotations, rye, and soil chemical treatments on management of plant-parasitic nematodes, thrips, and soilborne fungal diseases and on crop yield. peanut-cotton-rye rotations were conducted from 1988 to 1994 on tifton loamy sand (plinthic kandiudult) infested primarily ...199819274213
loss of avirulence and reduced pathogenicity of a gamma-irradiated mutant of fusarium oxysporum f. sp. lycopersici.abstract the tomato fusarium resistance gene i-2 confers resistance to f. oxy-sporum f. sp. lycopersici race 2, which expresses the corresponding aviru-lence gene avri-2. to elucidate the molecular basis of this gene-for-gene interaction, we initiated a search for the avri-2 gene. gamma irradiation mutagenesis, using (137)cs, was performed to generate an avri-2 mutant of f. oxysporum f. sp. lycopersici. to this end, a race 2 isolate was first transformed with a phleomycine resistance gene and a ...199918944636
detection of fusarium oxysporum f. sp. dianthi in carnation tissue by pcr amplification of transposon insertions.abstract strains of the carnation wilt pathogen, fusarium oxysporum f. sp. dianthi, can be distinguished by dna fingerprint patterns, using the fungal transposable elements fot1 and impala as probes for southern hybridization. the dna fingerprints correspond to three groups of f. oxysporum f. sp. dianthi strains: the first group includes isolates of races 1 and 8; the second group includes isolates of races 2, 5 and 6; and the third group includes isolates of race 4. genomic dnas flanking race-a ...199918944641
microbial antagonism at the root level is involved in the suppression of fusarium wilt by the combination of nonpathogenic fusarium oxysporum fo47 and pseudomonas putida wcs358.abstract two biological control agents, nonpathogenic fusarium oxysporum fo47 and pseudomonas putida wcs358, were evaluated for suppression of fusarium wilt of flax grown in nutrient solution and for suppression of the population density and metabolic activity of the causal organism f. oxysporum f. sp. lini strain foln3gus on root surfaces. due to the presence of an introduced gusa reporter gene construct in foln3gus, the pathogen expressed beta-glucuronidase activity that was related to its car ...199918944664
population genetic analysis corroborates dispersal of fusarium oxysporum f. sp. radicis-lycopersici from florida to europe.abstract fusarium oxysporum isolates from tomato plants displaying crown and root rot symptoms were collected in central and southern florida and analyzed using vegetative compatibility grouping (vcg) and nuclear restriction fragment length polymorphism (rflp) data. vcg 0094 of f. oxysporum f. sp. radicis-lycopersici, previously known only from northwestern europe, was predominant among 387 isolates assessed. in addition, two newly described vcgs (0098 and 0099) were detected at low frequencies. ...199918944673
spatial distribution and temporal development of fusarium crown and root rot of tomato and pathogen dissemination in field soil.abstract the spatial distribution and temporal development of tomato crown and root rot, caused by fusarium oxysporum f. sp. radicis-lycopersici, were studied in naturally infested fields in 1996 and 1997. disease progression fit a logistic model better than a monomolecular one. geostatistical analyses and semivariogram calculations revealed that the disease spreads from infected plants to a distance of 1.1 to 4.4 m during the growing season. by using a chlorate-resistant nitrate nonutilizing (n ...199918944713
ultrastructural and cytochemical aspects of the interaction between the mycoparasite pythium oligandrum and soilborne plant pathogens.abstract the interaction between the oomycete pythium oligandrum and various soilborne oomycete and fungal plant pathogens (p. ultimum, p. aphanidermatum, fusarium oxysporum f. sp. radicis-lycopersici, verticillium albo-atrum, rhizoctonia solani, and phytophthora megasperma) was studied by light and electron microscopy in order to assess the relative contribution of mycoparasitism and antibiosis in the antagonistic process. scanning electron microscope investigations of the interaction regions s ...199918944723
rapid detection of the fusarium oxysporum lineage containing the canary island date palm wilt pathogen.abstract fusarium oxysporum f. sp. canariensis causes fusarium wilt disease on the canary island date palm (phoenix canariensis). to facilitate disease management, a polymerase chain reaction diagnostic method has been developed to rapidly detect the pathogen. a partial genomic library of f. oxysporum f. sp. canariensis isolate 95-913 was used to identify a dna sequence diagnostic for a lineage containing all tested isolates of f. oxysporum f. sp. canariensis. two oligonucleotide primers were de ...199918944754
biological and molecular characterization of fusarium oxysporum f. sp. lycopersici divides race 1 isolates into separate virulence groups.abstract a collection of race 1 and race 2 isolates of fusarium oxysporum f. sp. lycopersici was screened for vegetative compatibility and characterized by random amplified polymorphic dna (rapd) analysis to establish the identity and genetic diversity of the isolates. comparison of rapd profiles revealed two main groups that coincide with vegetative compatibility groups (vcgs). in addition, several single-member vcgs were identified that could not be grouped in one of the two main rapd clusters ...199918944790
genetic diversity of fusarium oxysporum isolates from cucumber: differentiation by pathogenicity, vegetative compatibility, and rapd fingerprinting.abstract a total of 106 isolates of fusarium oxysporum obtained from diseased cucumber plants showing typical root and stem rot or fusarium wilt symptoms were characterized by pathogenicity, vegetative compatibility, and random amplified polymorphic dna (rapd). twelve isolates of other formae speciales and races of f. oxysporum from cucurbit hosts, three avirulent isolates of f. oxysporum, and four isolates of fusarium spp. obtained from cucumber were included for comparison. of the 106 isolates ...199918944791
biological control of fusarium wilt of cucumber by chitinolytic bacteria.abstract two chitinolytic bacterial strains, paenibacillus sp. 300 and streptomyces sp. 385, suppressed fusarium wilt of cucumber (cucumis sativus) caused by fusarium oxysporum f. sp. cucumerinum in nonsterile, soilless potting medium. a mixture of the two strains in a ratio of 1:1 or 4:1 gave significantly (p < 0.05) better control of the disease than each of the strains used individually or than mixtures in other ratios. several formulations were tested, and a zeolite-based, chitosan-amended f ...199918944809
in vitro evaluation on inhibitory nature of some neem formulations against plant pathogenic fungi.different neem formulations derived from the neem tree (azadirachta indica) have been found to be potential fungicides against a broad spectrum of plant pathogenic fungi. some neem formulations viz. achook (0.15% ec), bioneem (0.03% ec), nimbecidine (0.03% ec) and neemark (0.03% ec) were examined against some plant pathogenic fungi such as (fusarium oxysporum, alternaria solani, curvularia lunata, helminthosporium sp. and sclerotium rolfsii). among these achook (0.15% ec) was found to be more ac ...199912024976
[purification and characterization of antifungal peptide lp-1].an antifungal peptide lp-1 from bacillus subtilis tg26 strain was purified by acid precipitation, acetone precipitation and hi-pore reversed phase column chromatography. the molecular weight of lp-1 is 1057.3 d as determined by maldi-tof mass spectrometry, and its pi is 4.75 by pag-ief. it was also found to be thermostable. its antifungal spectrum showed that lp-1 has strong inhibitory activity against many plant pathogenic fungi, such as pythium aphanidermatum, gibberella zeae, alternaria longi ...199912555526
[preparation of immobilized penicillin v acylase from fusarium oxysporum and its properties].extracellular penicillin v acylase from fusarium oxysporum fp941 was partially purified by means of adsorption on, and elution from gamma-alumina and (hn4)2so4 fractional precipitation. the enzyme was immobilized on acrylic polymer support linkaged with covalent bond. the activity of wet immobilized enzyme was 217 iu/g, the activity yield of immobilized enzyme was 53%. optimum ph and temperature for immobilized enzyme action wer 8.0 and 55 degrees c, respectively. the enzyme was stable below 50 ...199912555562
substrate specificity of a nitroalkane-oxidizing enzyme.the flavoprotein nitroalkane oxidase from fusarium oxysporum catalyzes the oxidation of nitroalkanes to aldehydes with production of hydrogen peroxide and nitrite. the substrate specificity of the fad-containing enzyme has been determined as a probe of the active site structure. nitroalkane oxidase is active on primary and secondary nitroalkanes, with a marked preference for unbranched primary nitroalkanes. the v/k values for primary nitroalkanes increase with increasing length of the alkyl chai ...199910068453
n-acetyl derivatives of type c fumonisins produced by fusarium oxysporumfour new fumonisins, an isomer of fumonisin c1 (iso-fc1) (1) and three n-acetyl derivatives of hydroxylated fumonisin c1 (oh-fc1) (2), fumonisin c1 (fc1) (3), and iso-fc1 (4) were isolated from wheat cultures of fusarium oxysporum. one of the hydroxy groups of compound 1 is at c-3 instead of c-4 for fc1. compounds 2-4 are similar in structure to oh-fc1, fc1, and iso-fc1, respectively, except for the presence of an acetyl group at the amine of c-1.199910075786
phytotoxicity of pathogenic fungi and their mycotoxins to cereal seedling viability.aspergillus flavus, alternaria alternata and fusarium oxysporum were the pathogenic fungi most reduced cereal (barley, sorghum and wheat) seedlings. the pathogens have the ability to produce aflatoxin b1 and g1, diacetoxyscirpenol, kojic acid and tenuazonic acid that reduced seedling viability. the inhibition dose for 50% reduction (ld50) was recorded by aflatoxins at 0.83 mg l-1 for barley, 1.74 mg l-1 for wheat and 2.75 mg l-1 for sorghum. diacetoxyscirpenol produced its inhibition at 1.26 mg ...199911189766
biotechnological production of oil: fatty acid composition of microbial oil.the fatty acid profile and the fatty acid composition of microbial lipids obtained from molds revealed that oil from aspergillus sydowii, fusarium oxysporum and f. equisetti had a high percentage of unsaturated fatty acids, particularly oleic acid, and had a similarity to the edible oils, groundnut and palm oil. this study sheds light on the possibilities of exploring the use of these oils as supplement to other edible fats and for other non-edible industrial purposes.199910540990
a siderophore from pseudomonas putida type a1: structural and biological characterization.a siderophore from a root-colonizing, plant-beneficial fluorescent pseudomonas (p. putida type a1) isolated from chickpea rhizosphere was studied. culture conditions required for optimal production of the chromophore by the organism were standardized. the compound was purified by gel filtration, ion exchange and rp-hplc chromatographic procedures. the purified compound exhibited siderophore activity for p. putida and antifungal activity on phytopathogens, fusarium oxysporum f. sp. ciceri and hel ...199910561535
combined distal and lateral subungual and white superficial onychomycosis in the toenails.the 5 main types of onychomycosis are distal and lateral subungual onychomycosis (dlso), white superficial onychomycosis (wso), proximal subungual onychomycosis (pso), candida onychomycosis, and total dystrophic onychomycosis (tdo) (primary or secondary type). in the literature there is infrequent discussion about 2 types of onychomycosis present in the toenails of the same individual.199910570377
steroid transformations with fusarium oxysporum var. cubense and colletotrichum musae.the utility of two locally isolated fungi, pathogenic to banana, for steroid biotransformation has been studied. the deuteromycetes fusarium oxysporum var. cubense (imi 326069, uamh 9013) and colletotrichum musae (imi 374528, uamh 8929) had not been examined previously for this potential. in general, f. oxysporum var. cubense effected 7alpha hydroxylation on 3beta-hydroxy-delta5-steroids, 6beta, 12beta, and 15alpha hydroxylation on steroidal-4-ene-3-ones, side-chain degradation on 17alpha,21-dih ...199910576218
pseudomonas aeruginosa (grc1) as a strong antagonist of macrophomina phaseolina and fusarium oxysporum.a plant growth promotory bacterial strain, isolated from the potato rhizosphere, was characterized as pseudomonas aeruginosa (grc1). the isolate produced an hydroxamate type of siderophore after 48 h of incubation on tryptic soy medium under iron deficient conditions. the in vitro antifungal activity of p. aeruginosa was tested against two soil-borne plant pathogens, macrophomina phaseolina and fusarium oxysporum. the antagonistic behaviour of the isolate was tested by dual culture technique. th ...199910581709
cytochrome p450nor, a novel class of mitochondrial cytochrome p450 involved in nitrate respiration in the fungus fusarium oxysporum.fusarium oxysporum, an imperfect filamentous fungus performs nitrate respiration under limited oxygen. in the respiratory system, cytochrome p450nor (p450nor) is thought to catalyze the last step; reduction of nitric oxide to nitrous oxide. we examined its intracellular localization using enzymatic, spectroscopic, and immunological analyses to show that p450nor is found in both the mitochondria and the cytosol. translational fusions between the putative mitochondrial targeting signal on the amin ...199910600173
investigation of bioactivity of extracts from moroccan solitary tunicate cynthia savignyi.extracts of the tunicate cynthia savignyi from the moroccan atlantic sea have been investigated in five bioassays. the first is an antibacterial test against escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and agrobacterium tumefaciens; the second is an antifungal test against three tomato pathogenic fungi, botrytis cinerea, fusarium oxysporum and verticillium albo-atrum; the third is a test based to the ability to reduce dna peak size in procedures using an hplc system for detec ...199910624861
characterization of two novel type i ribosome-inactivating proteins from the storage roots of the andean crop mirabilis expansa.two novel type i ribosome-inactivating proteins (rips) were found in the storage roots of mirabilis expansa, an underutilized andean root crop. the two rips, named me1 and me2, were purified to homogeneity by ammonium sulfate precipitation, cation-exchange perfusion chromatography, and c4 reverse-phase chromatography. the two proteins were found to be similar in size (27 and 27.5 kd) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their isoelectric points were determined to be ...199910198104
specific expression of the fusarium transposon fot1 and effects on target gene transcription.the fot1 transposon is active in some strains of the plant pathogenic fungus fusarium oxysporum. in a high-copy-number strain that contains autonomous elements, we have detected a transcript of 1.7 kb hybridizing to fot1 in very low amounts. mapping the 3' and 5' termini of this transcript confirms that it corresponds to a fot1-specific transcript. in this strain, five independent mutants of the transgene (niad) encoding nitrate reductase have arisen by insertion of fot1 into the third intron. t ...199910200958
two xylanase genes of the vascular wilt pathogen fusarium oxysporum are differentially expressed during infection of tomato plants.two genes encoding putative family f xylanases from the tomato vascular wilt pathogen fusarium oxysporum f.sp. lycopersici have been cloned and sequenced. the two genes, designated xyl2 and xyl3, encode proteins with calculated molecular masses of 33 and 39.3 kda and isoelectric points of 8.9 and 6.7, respectively. the predicted amino acid sequences show significant homology to other family f xylanases. xyl3 contains a cellulose-binding domain in its n-terminal region. southern analysis suggeste ...199910323234
effects of methylprednisolone and cyclosporine a on fungal growth in vitro.the management of corneal transplants after mycotic keratitis often poses a therapeutic dilemma. clinicians are hesitant to use topical steroids because of their potential enhancement of fungal growth. this study seeks to evaluate the in vitro effects of methylprednisolone and cyclosporine a on the growth of various molds that often are responsible for keratomycoses.199910336034
ph regulation of recombinant glucoamylase production in fusarium venenatum jers 325, a transformant with a fusarium oxysporum alkaline (trypsin-like) protease promoter.fusarium venenatum (formerly fusarium graminearum) jers 325 produces heterologous glucoamylase (gam) under the regulation of a fusarium oxysporum alkaline (trypsin-like) protease promoter. the glucoamylase gene was used as a reporter gene to study the effects of ammonium and ph on gam production under the control of the alkaline protease promoter. between ph 4.0 and 5.8, gam production in glucose-limited chemostat cultures of jers 325 grown at a dilution rate of 0.10 h-1 (doubling time, 6.9 h) o ...199910397874
specific detection of fusarium species in blood and tissues by a pcr technique.fusarium species are opportunistic nosocomial pathogens that often cause fatal invasive mycoses. we designed a primer pair that amplifies by pcr a fragment of a gene coding for the rrna of fusarium species. the dnas of the main fusarium species and neocosmospora vasinfecta but not the dnas from 11 medically important fungi were amplified by these primers. the lower limit of detection of the pcr system was 10 fg of fusarium solani dna by ethidium bromide staining. to test the ability of this pcr ...199910405380
folyt1, a new member of the hat family, is active in the genome of the plant pathogen fusarium oxysporum.an active transposable element, folyt1, has been isolated from the tomato pathogen fusarium oxysporum f. sp. lycopersici as an insertion sequence within the coding region of the nitrate reductase gene (nit 1) in two independent mutants (co66 and co108). folyt1 was 2615 bp in length and contained 9-bp imperfect inverted terminal repeats (itrs) and 8 bp duplicated at the target site upon insertion. the element contained a long open reading frame interrupted by a single putative intron. the predict ...199910413616
genetic diversity of fusarium oxysporum strains from common bean fields in spain.fusarium wilt is an endemic disease in el barco de avila (castilla y león, west-central spain), where high-quality common bean cultivars have been cultured for the last century. we used intergenic spacer (igs) region polymorphism of ribosomal dna, electrophoretic karyotype patterns, and vegetative compatibility and pathogenicity analyses to assess the genetic diversity within fusarium oxysporum isolates recovered from common bean plants growing in fields around el barco de avila. ninety-six vege ...199910427016
production of a new d-amino acid oxidase from the fungus fusarium oxysporum.the fungus fusarium oxysporum produced a d-amino acid oxidase (ec 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. the specific d-amino acid oxidase activity was increased up to 12.5-fold with various d-amino acids or their corresponding derivatives as inducers. the best inducers were d-alanine (2.7 microkat/g of dry biomass) and d-3-aminobutyric acid (2.6 microkat/g of dry biomass). the addition of zinc ions was necessary to p ...199910427080
sensitivity of fusarium strains to chelidonium majus l. extracts.ten fusarium strains were tested for their sensitivity to extracts of chelidonium majus l. growth inhibition was measured either in solid or in liquid media. aqueous extracts had considerable inhibitory action but methanolic extracts showed the best results. root extracts were more inhibitory than shoot extracts. on the basis of growth inhibition the fusarium strains were aggregated into five classes, the extremes being fusarium culmorum plus fusarium graminearum (quite resistant) and fusarium o ...199910433471
the cell wall of fusarium oxysporum.sugar analysis of isolated cell walls from three formae speciales of fusarium oxysporum showed that they contained not only glucose and (n-acetyl)-glucosamine, but also mannose, galactose, and uronic acids, presumably originating from cell wall glycoproteins. cell wall glycoproteins accounted for 50-60% of the total mass of the wall. x-ray diffraction studies showed the presence of alpha-1, 3-glucan in the alkali-soluble cell wall fraction and of beta-1, 3-glucan and chitin in the alkali-insolub ...199910441453
in vitro and in vivo antagonism of pathogenic turfgrass fungi by streptomyces hygroscopicus strains yced9 and wye53.disease prevention is a current practice used to minimize fungal diseases of turfgrasses in lawns and golf greens. prevention is accomplished through fungicide applications, and by periodic thatch removal. during the development of a microbial biodethatch product utilizing the lignocellulose-degrading streptomyces hygroscopicus strains yced9 and wye53, we demonstrated using in vitro plate antagonism bioassays that both strains are antagonists of various turfgrass fungal pathogens. these activiti ...199910455494
biotransformation of protriptyline by filamentous fungi and yeasts.1. the potential of various fungi to metabolize protriptyline (an extensively used antidepressant) was studied to investigate similarities between mammalian and microbial metabolism. 2. metabolites produced by each organism were isolated by high-pressure liquid chromatography and identified by nuclear magnetic resonance and mass spectrometry. the metabolites identified in one or more fungi were 2-hydroxyprotriptyline, n-desmethylprotriptyline, n-acetylprotriptyline, n-acetoxyprotriptyline, 14-ox ...199910456691
mechanisms of coal solubilization by the deuteromycetes trichoderma atroviride and fusarium oxysporumthree different mechanisms can be envisaged that are used by fungi to solubilize coal: the production of alkaline substances, the extrusion of chelators and, of special interest in the scope of biotechnology, the action of enzymes. whether these mechanisms are operating separately or in various combinations has not yet been finally assessed. the two deuteromycetes fusarium oxysporum and trichoderma atroviride solubilize coal by synergistic effects of various differnt mechanisms depending on the ...199910461370
methoxybifurcarenone: an antifungal and antibacterial meroditerpenoid from the brown alga cystoseira tamariscifolia.a meroditerpenoid metabolite has been isolated from the brown alga cystoseira tamariscifolia and characterized as methoxybifurcarenone, by spectral analysis. methoxybifurcarenone possesses antifungal activity against three tomato pathogenic fungi: botrytis cinerea, fusarium oxysporum sp. mycopersici and verticillium alboatrum and antibacterial activity against agrobacterium tumefaciens and escherichia coli.199910466223
first chromatographic isolation of an antifungal thaumatin-like protein from french bean legumes and demonstration of its antifungal activity.a protein, with a molecular weight of 20 kda, and an n-terminal sequence analogous to those of thaumatin-like proteins (tlps) and thaumatins, was first isolated from the legume of the french bean phaseolus vulgaris cv kentucky wonder using a simple procedure involving affinity and ion exchange chromatography. the protein was adsorbed on both cm-sepharose and affi-gel blue gel. it was the first leguminous tlp-like protein demonstrated to exert antifungal activity against fusarium oxysporum, pleur ...199910486265
fungal utilization of organophosphate pesticides and their degradation by aspergillus flavus and a. sydowii in soil.fungal species were isolated which utilize organophosphate pesticides, viz. phosphorothioic (pirimiphos-methyl and pyrazophos), phosphorodithioic (dimethoate and malathion), phosphonic (lancer) and phosphoric (profenfos) acid derivatives. pesticide degradation was studied in vitro and in vivo (soil). aspergillus flavus, a. fumigatus, a. niger, a. sydowii, a. terreus, emericella nidulans, fusarium oxysporum and penicillium chrysogenum were isolated from pesticide-treated wheat straw. the number o ...199910489696
myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel mytilus galloprovincialis.we report here the isolation of two isoforms of a novel cysteine-rich peptide from haemocytes (isoform a of 4.438 da and b of 4.562 da) and plasma (isoform a) of the mussel, mytilus galloprovincialis. the two molecules display antibacterial activity against gram-positive bacteria, whereas only isoform b is active against the fungus fusarium oxysporum and a gram-negative bacteria escherichia coli d31. complete peptide sequences were determined by a combination of edman degradation, mass spectrome ...199910491159
tomatinase from fusarium oxysporum f. sp. lycopersici defines a new class of saponinases.plants produce a variety of secondary metabolites, many of which have antifungal activity. saponins are plant glycosides that may provide a preformed chemical barrier against phytopathogenic fungi. fusarium oxysporum f. sp. lycopersici and other tomato pathogens produce extracellular enzymes known as tomatinases, which deglycosylate alpha-tomatine to yield less toxic derivatives. we have cloned and characterized the cdna and genomic dna encoding tomatinase from the vascular pathogen of tomato f. ...199910517025
biochemical analysis of plant protection afforded by a nonpathogenic endophytic mutant of colletotrichum magnaa nonpathogenic mutant of colletotrichum magna (path-1) was previously shown to protect watermelon (citrullus lanatus) and cucumber (cucumis sativus) seedlings from anthracnose disease elicited by wild-type c. magna. disease protection was observed in stems of path-1-colonized cucurbits but not in cotyledons, indicating that path-1 conferred tissue-specific and/or localized protection. plant biochemical indicators of a localized and systemic (peroxidase, phenylalanine ammonia-lyase, lignin, and ...19999952476
cloning and characterization of pl1 encoding an in planta-secreted pectate lyase of fusarium oxysporum.a pectate lyase (pl1) from the tomato vascular wilt pathogen fusarium oxysporum f.sp. lycopersici was previously characterized, and evidence was obtained for its production in planta. the gene encoding pl1 was isolated from a genomic library of f. oxysporum f. sp. lycopersici. pl1 encodes a 240 amino-acid polypeptide with one putative n-glycosylation site and a 15 amino-acid n-terminal signal peptide. pl1 showed 89%, 67%, 55% and 56% identity with the products of the fusarium solani f.sp. pisi p ...199910022947
antibiosis plays a role in the context of direct interaction during antagonism of paenibacillus polymyxa towards fusarium oxysporum.interaction of fusarium oxysporum and paenibacillus polymyxa starts with polar attachment of bacteria to the fungal hyphae followed by the formation of a large cluster of non-motile cells embedded in an extracellular matrix in which the bacteria develop endospores. enumeration of fungal viable counts showed that less than one of 36,000 colony-forming units survived in paired cultures for 71 h. effective antagonism was not observed below ph5 and was specific for the bacterial species. development ...199910030009
transposition of the autonomous fot1 element in the filamentous fungus fusarium oxysporum.autonomous mobility of different copies of the fot1 element was determined for several strains of the fungal plant pathogen fusarium oxysporum to develop a transposon tagging system. two fot1 copies inserted into the third intron of the nitrate reductase structural gene (niad) were separately introduced into two genetic backgrounds devoid of endogenous fot1 elements. mobility of these copies was observed through a phenotypic assay for excision based on the restoration of nitrate reductase activi ...199910049918
a hemorrhagic factor (apicidin) produced by toxic fusarium isolates from soybean seeds.fifty-two isolates of fusarium species were obtained from soybean seeds from various parts of korea and identified as fusarium oxysporum, f. moniliforme, f. semitectum, f. solani, f. graminearum, or f. lateritium. these isolates were grown on autoclaved wheat grains and examined for toxicity in a rat-feeding test. nine cultures were toxic to rats. one of these, a culture of fusarium sp. strain kctc 16677, produced apicidin, an antiprotozoal agent that caused toxic effects in rats (including body ...19999872769
isolation of two antifungal compounds fromneolitsea cuipala and their activity against some plant pathogenic fungi.zeylanidine and zeylanicine isolated fromneolitsea cuipala fruit peels are found to be potential antifungal agents againsthelminthosporium oryzae, fusarium oxysporum, alternaria solani, curvularia varuciformis andcolletotrichum gleosporioides.199918461489
development and utility of cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflps) linked to the fom-2 fusarium wilt resistance gene in melon (cucumis melo l.).fusarium wilt, caused by fusarium oxysporum schlecht f. sp. melonis snyder & hans, is a worldwide soil-borne disease of melon (cucumis melo l.). resistance to races 0 and 1 of fusarium wilt is conditioned by the dominant gene fom-2. to facilitate marker-assisted backcrossing with selection for fusarium wilt resistance, we developed cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflp) markers by converting rapd markers e07 (a 1.25-kb band) and g17 (a ...199922665178
disease development following infection of tomato and basil foliage by airborne conidia of the soilborne pathogens fusarium oxysporum f. sp. radicis-lycopersici and f. oxysporum f. sp. basilici.abstract fusarium oxysporum f. sp. radicis-lycopersici, the causal agent of fusarium crown and root rot of tomato, and f. oxysporum f. sp. basilici, the causal agent of fusarium wilt in basil, are soilborne pathogens capable of producing conspicuous masses of macroconidia along the stem. the role of the airborne propagules in the epidemics of the disease in tomato plants was studied. in the field, airborne propagules of f. oxysporum f. sp. radicis-lycopersici were trapped with a selective medium ...200018943372
detection and cloning of the gene encoding a protein produced by nonpathogenic mutants of fusarium oxysporum.treatment of fusarium oxysporum with 5-azacytidine, a potent inhibitor of dna methylation, induced nonpathogenic mutants. analysis of the protein expression pattern by two-dimensional gel electrophoresis revealed a protein that is present in yeast-form cells of the mutants but absent in those of the wild-type strain. n-terminal amino acid analysis indicated that this protein is identical to a region of a polypeptide encoded by a cdna clone, sti35, previously identified as a heat shock gene in f. ...200016232860
role of cell wall-degrading enzymes in pathogenicity of fusarium oxysporum.fusarium oxysporum invades its host plants through the roots and colonizes the vascular system. it produces a great variety of cell-wall degrading enzymes (cwde), such as cellulases, xylanases, pectinases and proteases. our group has purified and characterized an endopolygalacturonase (pg1), two exopolygalacturonases (pg2 and pg3), an endoxylanase (xyl1) and an endo pectatelyase (pl1). we have isolated the following cwde-encoding genes: pg1, pgx4, pg5, xyl2, xyl3, prt1 and pl1. gene expression i ...200015762782
identification of an essential tyrosine residue in nitroalkane oxidase by modification with tetranitromethane.the flavoprotein nitroalkane oxidase from fusarium oxysporum catalyzes the oxidation of nitroalkanes to the respective aldehydes or ketones with production of nitrite and hydrogen peroxide. the enzyme is irreversibly inactivated by incubation with tetranitromethane, a tyrosine-directed reagent, at ph 7.3. the inactivation is time-dependent and shows first-order kinetics for two half-lives of inactivation. further inactivation can be achieved upon a second addition of tetranitromethane. a saturat ...200010653664
antifungal nitro compounds from skunk cabbage (lysichitum americanum) leaves treated with cupric chloride.two nitro compounds, 2-(4-methoxyphenyl)-1-nitroethane named as lysichitalexin and 2-(4-hydroxyphenyl)-1-nitroethane were isolated as stress metabolites from the leaves of lysichitum americanum hultén and st. john treated with cupric chloride. their structures were determined by spectroscopic methods and chemical reactions. the former compound showed antifungal activities against fusarium oxysporum and cladosporium herbarum. both compounds were isolated for the first time from this species and t ...200010656408
dolichin, a new chitinase-like antifungal protein isolated from field beans (dolichos lablab).an antifungal protein, possessing a molecular weight of 28 kda and an n-terminal sequence resembling chitinases, has been purified from the seeds of the field bean dolichos lablab. the procedure involved extraction with aqueous buffer, affinity chromatography on affi-gel blue gel, and ion exchange chromatography on cm-sepharose. the protein, designated dolichin, exhibited antifungal activity against the fungi fusarium oxysporum, rhizoctonia solani, and coprinus comatus. dolichin was capable of i ...200010694493
quinqueginsin, a novel protein with anti-human immunodeficiency virus, antifungal, ribonuclease and cell-free translation-inhibitory activities from american ginseng roots.a homodimeric protein designated quinqueginsin, with a molecular weight of 53 kda, has been isolated from the roots of american ginseng panax quinquefolium. it was unadsorbed on deae cellulose in low ionic strength and neutral ph, and adsorbed on affigel blue gel and sp-sepharose under similar conditions. its n-terminal sequence bore similarity to those of plant ribosome inactivating proteins and fungal ribonucleases. the protein displayed a variety of biological activities. it possessed ribonuc ...200010694500
fusarium species from nepalese rice and production of mycotoxins and gibberellic acid by selected species.infection of cereal grains with fusarium species can cause contamination with mycotoxins that affect human and animal health. to determine the potential for mycotoxin contamination, we isolated fusarium species from samples of rice seeds that were collected in 1997 on farms in the foothills of the nepal himalaya. the predominant fusarium species in surface-disinfested seeds with husks were species of the gibberella fujikuroi complex, including g. fujikuroi mating population a (anamorph, fusarium ...200010698766
tobramycin-responsive fusarium oxysporum keratitis. 200010711382
biological control of fusarial wilt of pigeon pea by bacillus brevis.a virulent strain of pigeon pea wilt pathogen was isolated from wilted pigeon pea plants and was identified as fusarium oxysporum f. sp. udum. many bacterial cultures showing antagonism to the pathogen were isolated from various ecological niches. when tested under pot and field conditions, development of fusarial wilt symptoms was prevented in pigeon pea seeds treated with one such antagonist, bacillus brevis. a formulation of b. brevis with vermiculite as a carrier had a shelf life of at least ...200010721480
growth-rate-independent production of recombinant glucoamylase by fusarium venenatum jers 325.most recombinant proteins generated in filamentous fungi are produced in fed-batch cultures, in which specific growth rate normally decreases progressively with time. because of this, such cultures are more suited to the production of products that are produced efficiently at low-growth rates (e.g., penicillin) than to products which are produced more efficiently at high-growth rates (e. g., glucoamylase). fusarium venenatum a3/5 has been transformed (jers 325) to produce aspergillus niger gluco ...200010745192
cloning and disruption of pgx4 encoding an in planta expressed exopolygalacturonase from fusarium oxysporum.fusarium oxysporum f. sp. lycopersici, the causal agent of tomato vascular wilt, produces an array of pectinolytic enzymes, including at least two exo-alpha1,4-polygalacturonases (exopgs). a gene encoding an exopg, pgx4, was isolated with degenerate polymerase chain reaction primers derived from amino acid sequences conserved in two fungal exopgs. pgx4 encodes a 454 amino acid polypeptide with nine potential n-glycosylation sites and a putative 21 amino acid n-terminal signal peptide. the deduce ...200010755298
degradation of the metal-cyano complex tetracyanonickelate (ii) by fusarium oxysporum n-10.a fungus with the ability to utilize a metalcyano compound, tetracyanonickelate (ii) ¿k2[ni (cn)4]; tcn¿, as its sole source of nitrogen was isolated from soil and identified as fusarium oxysporum n-10. both intact mycelia and cell-free extract of the strain catalyzed hydrolysis of tcn to formate and ammonia and produced formamide as an intermediate, thereby indicating that a hydratase and an amidase sequentially participated in the degradation of tcn. the enzyme catalyzing the hydration of tcn ...200010772474
foxy: an active family of short interspersed nuclear elements from fusarium oxysporum.a novel family of short interspersed nuclear elements (sines) has been identified in fusarium oxysporum. this family has been called foxy. the feature that makes foxy unique among sines is the presence of 5' terminal tetranucleotide repeats. both the number and the sequence of these repeats vary between individual members of the family. the genome of f. oxysporum f. sp. lycopersici contains at least 160 copies of foxy. in a mutant obtained upon gamma irradiation of a wild-type isolate, 13 new fo ...200010778745
nitric oxide reduction, the last step in denitrification by fusarium oxysporum, is obligatorily mediated by cytochrome p450nor.the involvement of cytochrome p450nor (p450nor) is the most striking feature of the fungal denitrifying system, and has never been shown in bacterial systems. to establish the physiological significance of the p450nor, we constructed and investigated mutants of fusarium oxysporum that lacked the gene for p450nor. we mutated the gene by targeted integration of a disrupted gene into the chromosome of f. oxysporum. the mutants were shown to contain neither p450nor protein nor nitric oxide (no) redu ...200010778754
fungicidal activity of natural and synthetic sesquiterpene lactone analogs.fungicidal activity of 36 natural and synthetic sesquiterpene lactones with guaianolide, trans, trans-germacranolide, cis, cis-germacranolide, melampolide, and eudesmanolide carbon skeletons was evaluated against the phytopathogenic fungi colletotrichum acutatum, c. fragariae, c. gloeosporioides, fusarium oxysporum, botrytis cinerea, and phomopsis sp. dose-response data for the active compounds dehydrozaluzanin c, dehydrocostuslactone, 5alpha-hydroxydehydrocostuslacone, costunolide, and zaluzani ...200010783981
molecular characterization and identification of biocontrol isolates of trichoderma spp.the most common biological control agents (bcas) of the genus trichoderma have been reported to be strains of trichoderma virens, t. harzianum, and t. viride. since trichoderma bcas use different mechanisms of biocontrol, it is very important to explore the synergistic effects expressed by different genotypes for their practical use in agriculture. characterization of 16 biocontrol strains, previously identified as "trichoderma harzianum" rifai and one biocontrol strain recognized as t. viride, ...200010788356
isolation and identification of antifungal n-butylbenzenesulphonamide produced by pseudomonas sp. ab2.an antifungal bacterial strain, isolated from a greenhouse soil sample, inhibits growth of microflora nearby. it was selected for further studies of bacterial antifungal properties. this isolate was identified as a pseudomonas sp. based on carbohydrate utilization, and other biochemical and physiological tests. petri plate assay revealed that the pseudomonas sp. exhibited antifungal activity against the plant pathogens, pythium ultimum, rhizoctonia solani, phytophthora capsici, botrytis cinerea ...200010805572
comparison of chitinase isozymes from yam tuber--enzymatic factor controlling the lytic activity of chitinases.to evaluate the anti-pathogen activity of chitinases, we developed a new method for measuring the lytic activity, and investigated the correlation of the lytic activity with the enzymatic properties by using four chitinase isozymes, chitinases e, f, h1 and g, which had been purified from yam tubers by column chromatography. chitinases e, f and h1 had high lytic activity against the plant pathogen, fusarium oxysporum, but chitinase g did not. chitinase e, which is the family 19 chitinase, was sim ...200010830483
hybridization and breeding of the benomyl resistant mutant, trichoderma harziantum antagonized to phytopathogenic fungi by protoplast fusion.a diploid strain obtained from heterokaryons of trichoderma harzianum by protoplast fusion grew on minimal medium containing 100ppm benomyl. this strain inhibited the growth of the phytopathogenic fungus fusarium oxysporum f. sp. raphani on paired cultures and also protected against radish yellows and a drop in germination induced by f. oxysporum f. sp. raphani.200010830500
genome organization in fusarium oxysporum: clusters of class ii transposons.several families of transposable elements (tes) are present in the genome of the phytopathogenic fungus fusarium oxysporum. they are present in copy numbers ranging from just a few elements to tens or hundreds per genome. sequence analysis of contiguous stretches of genomic dna surrounding insertion sites of one family revealed that they are packed with repeated sequences. we have carried out a detailed study of the composition and arrangement of these repeats in three chromosomal regions. we fo ...200010853772
nitrate reductase and nitrous oxide production by fusarium oxysporum 11dn1 under aerobic and anaerobic conditions.the fungus fusarium oxysporum 11dn1 was found to be able to grow and produce nitrous oxide on nitrate-containing medium in anaerobic conditions. the rate of nitrous oxide formation was three to six orders of magnitude lower than the rates of molecular nitrogen production by common denitrifying bacteria. acetylene and ammonia did not affect the release of nitrous oxide release. it was shown that under anaerobic conditions fast increase of nitrate reductase activity occurred, caused by the synthes ...200010856377
effect of common food preservatives on mycelial growth and spore germination of fusarium oxysporum.the growth and spore germination inhibition of fusarium oxysporum f.sp. radicis-cucumerinum by the common food additives: acetic acid, formic acid potassium sorbate, propionic acid, sorbic acid, and the fungistatic agent sec-butylamine was examined in vitro. the inhibitory efficacy of these chemicals decreased in the following order: sorbic acid, potassium sorbate, propionic acid, acetic acid, sec-butylamine and formic acid. at ph 6.4, the ed50 value for mycelium growth was: 976 ppm for sorbic a ...200010874628
mungin, a novel cyclophilin-like antifungal protein from the mung bean.a protein designated mungin, isolated from mung bean (phaseolus mungo) seeds, possessed activity against the fungi rhizoctonia solani, coprinus comatus, mycosphaerella arachidicola, botrytis cinerea, and fusarium oxysporum. the 18-kda protein also possessed a novel n-terminal sequence with similarity to cyclophilins. it exerts an inhibitory action against alpha- and beta-glucosidases suppresses [(3)h]thymidine in corporation by mouse splenocytes.200010891380
comparison of nccls and 3-(4,5-dimethyl-2-thiazyl)-2, 5-diphenyl-2h-tetrazolium bromide (mtt) methods of in vitro susceptibility testing of filamentous fungi and development of a new simplified method.the susceptibility of 30 clinical isolates belonging to six different species of filamentous fungi (aspergillus fumigatus, aspergillus flavus, scedosporium prolificans, scedosporium apiospermum, fusarium solani, and fusarium oxysporum) was tested against six antifungal drugs (miconazole, voriconazole, itraconazole, ur9825, terbinafine, and amphotericin b) with the microdilution method recommended by the national committee for clinical laboratory standards (nccls) (m38-p). the mics were compared ...200010921957
cloning and sequencing of an endoglucanase gene from scopulariopsis brevicaulis tof-1212, and its expression in saccharomyces cerevisiae.the egi gene, encoding a major endoglucanase (egi) of scopulariopsis brevicaulis tof-1212, was cloned and sequenced. the eglgene consisted of 868 bp with one intron and encoded a protein of 229 amino acids with a calculated molecular mass of 22,392 daltons. the egi was assigned to a family 45 of glycosyl hydrolases and showed high similarity with other fungal endoglucanases, especially with those of humicola grisea and fusarium oxysporum, on the basis of hydrophobic cluster analysis. the egi gen ...200010923796
purification and characterization of an extracellular alpha-l-arabinofuranosidase from fusarium oxysporum.an alpha-l-arabinofuranosidase from fusarium oxysporum f3 was purified to homogeneity by a two-step ion exchange intercalated by a gel filtration chromatography. the enzyme had a molecular mass of 66 kda and was optimally active at ph 6.0 and 60 degrees c. it hydrolyzed aryl alpha-l-arabinofuranosides and cleaved arabinosyl side chains from arabinoxylan and arabinan. there was a marked synergistic effect between the alpha-l-arabinofuranosidase and an endo-(1-->4)-beta-d-xylanase produced by f. o ...200010949693
sativin: a novel antifungal miraculin-like protein isolated from legumes of the sugar snap pisum sativum var. macrocarpon.an antifungal protein designated sativin was isolated from the legumes of the sugar snap (also known as honey pea) pisum sativum var. macrocarpon. the procedure entailed extraction, affinity chromatography on affi-gel blue gel and ion exchange chromatography on cm-sepharose. the protein exhibited a molecular weight of 38 kda in sds-polyacrylamide gel electrophoresis. it possessed an n-terminal amino acid sequence which showed similarity to those of miraculin (a sweet protein) and pisavin (a ribo ...200010968407
targeted disruption of sti35, a stress-responsive gene in phytopathogenic fungus fusarium oxysporum.sti35 is one of the heat-shock genes in fusarium oxysporum, which is a fungal pathogen for wilt disease in plants. we have isolated a genomic clone of sti35 and used it to create disruption mutations. disruption of the sti35 coding region resulted in the loss of a 32-kda protein present in heat-shocked cells. the disruption had no detectable effect on growth and development at various temperatures, nor on the ability to acquire thermotolerance in nutrient medium. but the sti35 disruptants showed ...200010977897
purification of a soluble cytochrome p450 from trichosporon montevideense.the yeast trichosporon montevideense cbs 6721 expressed large amounts of cytochrome p450 after cultivation in a glucose-peptone medium. the p450, which could be detected in the cytosolic fraction after cell breakage and ultracentrifugation, was purified to electrophoretic homogeneity and migrated in sds-page with a m(r) of 43,000. as indicated by ief, the preparation consisted of two different p450 isoforms with pi-values of 5.9 and 6.2, which were named p450ms1 and p450ms2 respectively. both is ...200010986675
fusarium oxysporum fatty-acid subterminal hydroxylase (cyp505) is a membrane-bound eukaryotic counterpart of bacillus megaterium cytochrome p450bm3.the gene of a fatty-acid hydroxylase of the fungus fusarium oxysporum (p450foxy) was cloned and expressed in yeast. the putative primary structure revealed the close relationship of p450foxy to the bacterial cytochrome p450bm3, a fused protein of cytochrome p450 and its reductase from bacillus megaterium. the amino acid sequence identities of the p450 and p450 reductase domains of p450foxy were highest (40.6 and 35.3%, respectively) to the corresponding domains of p450bm3. recombinant p450foxy e ...200010995755
development of formulations of biological agents for management of root rot of lettuce and cucumber.the effect of various carrier formulations of bacillus subtilis and pseudomonas putida were tested on germination, growth, and yield of lettuce and cucumber crops in the presence of pythium aphanidermatum and fusarium oxysporum f.sp. cucurbitacearum, respectively. survival of b. subtilis and p. putida in various carriers under refrigeration (about 0 degree c) and at room temperature (about 22 degrees c) was also studied. in all carrier formulations, b. subtilis strain bact-0 survived up to 45 da ...200011006841
potassium and sodium fluxes in the phytopathogenic fungus fusarium oxysporum var. lini.rubidium uptake in potassium-starved cells followed biphasic kinetics in the micromolar and millimolar range and was independent of the temperature. in contrast, rb(+) uptake in normal-k(+) cells followed a monophasic kinetics in the millimolar range and increased at temperatures higher than 30 degrees c. differences in the k(m) values and in the arrhenius plots of rb(+) uptake suggest different uptake systems in k(+)-starved and in normal-k(+) cells. in addition, the substantial inhibition of r ...200011014875
biolistic co-transformation of metarhizium anisopliae var. acridum strain cg423 with green fluorescent protein and resistance to glufosinate ammonium.metarhizium anisopliae var. acridum (syn. m. flavoviride) is recognized as a highly specific and virulent mycopathogen of locusts and grasshoppers and is currently being developed as a biological control agent for this group of insects in brazil. intact conidia of m. anisopliae var. acridum strain cg423 were transformed using microparticle bombardment. plasmids used were: (1) pbarks1 carrying the bar gene of streptomyces hygroscopicus fused to the aspergillus nidulans trpc promoter, encoding res ...200011024271
detection and substrate selectivity of new microbial d-amino acid oxidases.in order to screen for new microbial d-amino acid oxidase activities a selective and sensitive peroxidase/o-dianisidine assay, detecting the formation of hydrogen peroxide was developed. catalase, which coexists with oxidases in the peroxisomes or the microsomes and, which competes with peroxidase for hydrogen peroxide, was completely inhibited by o-dianisidine up to a catalase activity of 500 nkat ml(-)(1). thus, using the peroxidase/o-dianisidine assay and employing crude extracts of microorga ...200011024524
crystal structures of cytochrome p450nor and its mutants (ser286-->val, thr) in the ferric resting state at cryogenic temperature: a comparative analysis with monooxygenase cytochrome p450s.cytochrome p450nor (p450nor) is a heme enzyme isolated from the denitrifying fungus fusarium oxysporum and catalyzes the no reduction to n2o. crystal structures of the wild type and two ser286 mutants (ser286-->val, ser286-->thr) of p450nor have been determined for the ferric resting forms at a 1.7 a resolution at cryogenic temperature (100 k). we carried out three comparative analyses: (1) between the structures of p450nor at room temperature and cryogenic temperature, (2) between the structure ...200011051564
the sss colonization gene of the tomato-fusarium oxysporum f. sp. radicis-lycopersici biocontrol strain pseudomonas fluorescens wcs365 can improve root colonization of other wild-type pseudomonas spp.bacteria.we show that the disease tomato foot and root rot caused by the pathogenic fungus fusarium oxysporum f. sp. radicis-lycopersici can be controlled by inoculation of seeds with cells of the efficient root colonizer pseudomonas fluorescens wcs365, indicating that strain wcs365 is a biocontrol strain. the mechanism for disease suppression most likely is induced systemic resistance. p. fluorescens strain wcs365 and p. chlororaphis strain pcl1391, which acts through the production of the antibiotic ph ...200011059484
synthesis and antifungal activity of novel bisdithiocarbamate derivatives of carbohydrates against fusarium oxysporum f. sp. lini.novel carbamic esters possessing a carbohydrate moiety derived from glycerol or d-glucose with two n,n-diethyldithiocarbamoyl groups and a series of bisdithiocarbamic esters having a ketone or an alkyl ester have been synthesized. the in vitro activity of these new compounds was evaluated against fusarium oxysporum f. sp. lini. some of the compounds [bis[1,3-s-(n,n-diethyldithiocarbamoyl)]-1, 3-dideoxyglycerol) and diethyl n,n'-(1,3-dideoxyglycer-1, 3-diyl)bis(dithiocarbamate)] were more active ...200011087473
case report. pathohistological findings in a clinical case of disseminated infection with fusarium oxysporum.despite appropriate antimicrobial and antifungal therapy (amphotericin b), a disseminated infection with fusarium oxysporum in a 75-year-old immunocompromised patient (acute myeloid leukaemia, minimal leucocyte count of 0.5 giga l-1) led rapidly to death. a similarly fatal course of an f. oxysporum infection has been reported in several cases. fusarium oxysporum could be isolated shortly before death from blood cultures and from a swab taken from skin efflorescences. an autopsy revealed histopat ...200011105540
mating-type genes from asexual phytopathogenic ascomycetes fusarium oxysporum and alternaria alternata.mating-type (mat) loci were cloned from two asexual (mitosporic) phytopathogenic ascomycetes, fusarium oxysporum (a pyrenomycete) and alternaria alternata (a loculoascomycete), by a polymerase chain reaction (pcr)-based strategy. the conserved high mobility group (hmg) box domain found in the mat1-2-1 protein was used as a starting point for cloning and sequencing the entire mat1-2 idiomorph plus flanking regions. primer pairs designed to both flanking regions were used to amplify the opposite m ...200011106025
root colonization by phenazine-1-carboxamide-producing bacterium pseudomonas chlororaphis pcl1391 is essential for biocontrol of tomato foot and root rot.the phenazine-1-carboxamide-producing bacterium pseudomonas chlororaphis pcl1391 controls tomato foot and root rot caused by fusarium oxysporum f. sp. radicislycopersici. to test whether root colonization is required for biocontrol, mutants impaired in the known colonization traits motility, prototrophy for amino acids, or production of the site-specific recombinase, sss/xerc were tested for their root tip colonization and biocontrol abilities. upon tomato seedling inoculation, colonization muta ...200011106026
effects of mesophilic and thermophilic composts on suppression of fusarium root and stem rot of greenhouse cucumber.three composts were tested for their ability to suppress root and stem rot caused by the soil borne fungal pathogen fusarium oxysporum f. sp. radicis-cucumerinum (forc) on cucumber. two of the composts were prepared from separated dairy solids either by windrow (wds) or vermicomposting (vmc) while the third, obtained from international bio-recovery (ibr), was prepared from vegetable refuse using aerobic digestion. three sets of potting mixes were prepared by mixing the composts with sawdust at v ...200011109490
a robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive allium tuberosum.from the inner shoots of the chive allium tuberosum, a single-chained protein with a molecular weight of 36 kda and an n-terminal sequence manifesting resemblance to chitinases but lacking in cysteine residues characteristic of a cysteine-rich domain present in chitinases of other allium species, was purified. the isolation procedure entailed affinity chromatography on affi-gel blue gel, ion-exchange chromatography on deae-cellulose and mono s, and gel filtration on superdex 75. the protein was ...200011112420
ginkbilobin, a novel antifungal protein from ginkgo biloba seeds with sequence similarity to embryo-abundant protein.a novel single-chained antifungal protein with a molecular weight of 13 kda displaying an n-terminal sequence with marked similarity to embryo-abundant protein from the white spruce was isolated from the seeds of ginkgo biloba using ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-sepharose, and then gel filtration on superdex 75. the protein, designated ginkbilobin, exerted potent antifungal activity against a variety ...200011118300
surface hydrophobicity, viability and efficacy in biological control of penicillium oxalicum spores produced in aerial and submerged culture.the surface hydrophobicity, viability and biocontrol ability of penicillium oxalicum spores, produced either in aerial or submerged culture, were characterized. a phase distribution test showed that spores produced in both methods of culture were highly hydrophobic, but those produced in aerial culture were more hydrophobic. spores stored fresh at either 4 or 25 degrees c retained a high viability (80%) after 27 weeks of storage, although aerial spores survived better. freeze-drying severely aff ...200011119160
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