host-induced silencing of pathogenicity genes enhances resistance to fusarium oxysporum wilt in tomato.this study presents a novel approach of controlling vascular wilt in tomato by rnai expression directed to pathogenicity genes of fusarium oxysporum f. sp. lycopersici. vascular wilt of tomato caused by fusarium oxysporum f. sp. lycopersici leads to qualitative and quantitative loss of the crop. limitation in the existing control measures necessitates the development of alternative strategies to increase resistance in the plants against pathogens. recent findings paved way to rnai, as a promisin ...201728674943
antifungal activity and expression patterns of extracellular chitinase and β-1,3-glucanase in wickerhamomyces anomalus eg2 treated with chitin and this study, the expression patterns of extracellular chitinase and β-1,3-glucanase from cultured wickerhamomyces anomalus eg2 treated with chitin, glucan, and chemical chitinase inhibitors (kinetin, caffeine, and acetazolamide) were investigated using sds-page. relationship between enzyme expression and antifungal activity from yeast plays a very important role for biocontrol of phytopathoges. to determine antifungal activity against phytopathogens, w. anomalus eg2 was shown to strongly inhib ...201728668604
genetic diversity and antifungal susceptibility of fusarium isolates in onychomycosis.fusarium species have emerged as an important human pathogen in skin disease, onychomycosis, keratitis and invasive disease. onychomycosis caused by fusarium spp. the infection has been increasingly described in the immunocompetent and immunosuppressed hosts. considering onychomycosis is a difficult to treat infection, and little is known about the genetic variability and susceptibility pattern of fusarium spp., further studies are necessary to understand the pathogenesis and better to define th ...201728657120
novel loop-mediated isothermal amplification (lamp) assay with a universal qprobe can detect snps determining races in plant pathogenic fungi.tomato wilt pathogen fusarium oxysporum f. sp. lycopersici (fol) is grouped into three races based on their pathogenicity to different host cultivars. rapid detection and discrimination of fol races in field soils is important to prevent tomato wilt disease. although five types of point mutations in secreted in xylem 3 (six3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. the aim of this study is t ...201728652587
an evaluation method for suppression of pathogenic fusarium oxysporum by soil microorganisms using the dilution plate technique.volume 31, no. 3, page 307-313, 2016page 310, legend for fig. 4 incorrectfig. 4. growth degree of fusarium oxysporum f. sp. spinaciae for organic fertilizers at each dilution based on an estimation of the ellipse area (a) and extension length (b) of the colony. cont, compound inorganic fertilizer; sbm, steamed bone meal; cdc, cow dung compost; mi, microbial inoculant. values show mean of medians of degrees with se (n=3). the ellipse area and extension length for control plates were 4,475 mm(2) a ...201728652549
a meta-analysis of quantitative trait loci for abiotic and biotic stress resistance in tetraploid cotton.the number and location of mapped quantitative trait loci (qtl) depend on genetic populations and testing environments. the identification of consistent qtl across genetic backgrounds and environments is a pre-requisite to marker-assisted selection. this study analyzed a total of 661 abiotic and biotic stress resistance qtl based on our previous work and other publications using the meta-analysis software biomercator. it identified chromosomal regions containing qtl clusters for different resist ...201728647758
identification of a potential isr determinant from pseudomonas aeruginosa pm12 against fusarium wilt in tomato.biocontrol of plant diseases through induction of systemic resistance is an environmental friendly substitute to chemicals in crop protection measures. different biotic and abiotic elicitors can trigger the plant for induced resistance. present study was designed to explore the potential of pseudomonas aeruginosa pm12 in inducing systemic resistance in tomato against fusarium wilt. initially the bioactive compound, responsible for isr, was separated and identified from extracellular filtrate of ...201728620396
different mechanisms of trichoderma virens-mediated resistance in tomato against fusarium wilt involve the jasmonic and salicylic acid the present study, we investigated the role of trichoderma virens (triv_jsb100) spores or cell-free culture filtrate in the regulation of growth and activation of the defence responses of tomato (solanum lycopersicum) plants against fusarium oxysporum f. sp. lycopersici by the development of a biocontrol-plant-pathogen interaction system. two-week-old tomato seedlings primed with triv_jsb100 spores cultured on barley grains (bgs) or with cell-free culture filtrate (cf) were inoculated with fu ...201728605157
the arabidopsis leucine-rich repeat receptor kinase mik2/lrr-kiss connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses.plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. however, few components involved in plant cell wall integrity sensing have been described to date. using a reverse-genetic approach, we identified the arabidopsis thaliana leucine-rich repeat receptor kinase mik2 as an important regulator of cell wall damage responses triggered upon cellulose biosynthesis inhibition. indeed, loss-of-function mik2 alleles are strongly ...201728604776
fusarium infection causes genotoxic disorders and antioxidant-based damages in orobanche spp.this study aims to evaluate the toxic effects of fusarium oxysporum on root parasitic weed, orobanche spp. comparative genetic and gene expression studies were conducted on uninfected and fungus-infected orobanches. in genetic studies, isolated total dna was amplified by rapd pcr. fragment properties were analysed by gts test. according to the results, the fragment properties of control and fusarium infected (experimental) groups varied widely; and it has been observed that fusarium has genotoxi ...201728602401
in vitro and in vivo antifungal efficacy of plant based lawsone against fusarium oxysporum species complex.fusarium oxysporum is an ascomycete facultative fungus which generally affects to plants. however, it is recently known as a serious emerging opportunistic pathogen of human and other animals. f. oxysporum shows broad resistance to commonly used antifungal agents and therefore development of alternative therapeutic agents is required. in this study, we investigated the antifungal efficacy of plant based natural lawsone against pathogenic f. oxysporum. antifungal susceptibility test determined th ...201728602398
purification, characterization and retting of crotolaria juncea fibres by an alkaline pectin lyase from fusarium oxysporum mtcc 1755.using solid-state fermentation, production of an industrially important pectin lyase from a fungal strain fusarium oxysporum mtcc 1755 was attempted, which was further subjected to purification and characterization. the enzyme was purified by three steps, namely ammonium sulfate fractionation, cation-exchange chromatography on cm cellulose followed by gel filtration chromatography using sephadex g-100 column. a 16-fold purification with 31.2% yield and 3.2 u/mg specific activity was achieved. th ...201728593518
polyketide-terpene hybrid metabolites from an endolichenic fungus pestalotiopsis sp.five new polyketide-terpene hybrid metabolites (1-5) with highly functionalized groups, together with six known derivatives (6-11), were isolated from the endolichenic fungus pestalotiopsis sp. their structures were elucidated by extensive nmr experiments including (1)h, (13)c, hmqc, cosy, and hmbc. the relative configurations of the new compounds were determined by analysis of coupling constants and roesy correlations. the absolute configurations especially the secondary alcohol at c-15 in 1 an ...201728593175
specific pcr detection of fusarium oxysporum f. sp. raphani: a causal agent of fusarium wilt on radish plants.fusarium oxysporum, a causal agent of fusarium wilt, is one of the most important fungal pathogens worldwide, and detection of f. oxysporum dna at the forma specialis level is crucial for disease diagnosis and control. in this study, two novel f. oxysporum f. sp. raphani (for)-specific primer sets were designed, for1-f/for1-r and for2-f/for2-r, to target foqg_17868 and foqg_17869 orfs, respectively, which were selected based on the genome comparison of other formae speciales of f. oxysporum incl ...201728585248
biodiversity in targeted metabolomics analysis of filamentous fungal pathogens by (1)h nmr-based studies.the taxonomical classification among fungi kingdom in the last decades was evolved. in this work the targeted metabolomics study based on (1)h nmr spectroscopy combined with chemometrics tools was reported to be useful for differentiation of three model of fungal strains, which represent various genus of ascomycota (aspergillus pallidofulvus, fusarium oxysporum, geotrichum candidum) were selected in order to perform metabolomics studies. each tested species, revealed specific metabolic profile o ...201728585165
corrigendum: transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.[this corrects the article on p. 362 in vol. 8, pmid: 28367157.].201728572815
an allene oxide and 12-oxophytodienoic acid are key intermediates in jasmonic acid biosynthesis by fusarium oxysporum.fungi can produce jasmonic acid (ja) and its isoleucine conjugate in large quantities, but little is known about the biosynthesis. plants form ja from 18:3n-3 by 13s-lipoxygenase (lox), allene oxide synthase, and allene oxide cyclase. shaking cultures of fusarium oxysporum f. sp. tulipae released over 200 mg of jasmonates per liter. nitrogen powder of the mycelia expressed 10r-dioxygenase-epoxy alcohol synthase activities, which was confirmed by comparison with the recombinant enzyme. the 13s-lo ...201728572515
tobacco rattle virus-based silencing of enoyl-coa reductase gene and its role in resistance against cotton wilt disease.a tobacco rattle virus (trv)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (gossypium hirsutum). this approach was used to investigate the function of the enoyl-coa reductase (ghecr) gene in pathogen defense. amino acid sequence alignment of arabidopsis ecr with homologous sequence from g. hirsutum, g. arboreum, g. herbaceum and g. barbadense showed that ecrs are highly conserved among these species. trv-based silencing of gh ...201728567686
illumina-based sequencing analysis directed selection for actinobacterial probiotic candidates for banana potential probiotic candidates, plant vertically transmitted actinobacteria are beneficial to growth and health of host plants. new methods to isolate the actinobacterial taxa with low growth rates should be developed. based on the actinobacterial population information, the probiotic actinobacterial taxa could be directly isolated from healthy banana shoot tips. however, actinobacterial dnas with high gc contents could bias estimates of actinobacteria by pcr. in the study, two amplicon seque ...201728560514
small rna sequencing for secondary metabolite analysis in persicaria minor.persicaria minor (kesum) is an important medicinal plant and commonly found in southeast countries; malaysia, thailand, indonesia, and vietnam. this plant is enriched with a variety of secondary metabolites (sms), and among these sms, terpenoids are in high abundance. terpenoids are comprised of many valuable biomolecules which have well-established role in agriculture and pharmaceutical industry. in p. minor, for the first time, we have generated small rnas data sets, which can be used as tool ...201728560169
clean up fly ash from coal burning plants by new isolated fungi fusarium oxysporum and penicillium turkey approximately 45 million tons of coals are burned in a year and 19.3 million tons of fly ash have emerged. the bioremediation of heavy metals or different elements from fly ash makes them bio-available. however, in previous studies, requiring of long operational time and failing to show tolerance to high pulp densities of fly ash of selected fungal species makes them impractical. in this work, bioremediation of fly ash by new isolated fungi fusarium oxysporum and penicillium glabrum we ...201728554126
analysis of protein profile of tomato root infected with fusarium oxysporum f. sp lycopersici.fusarium wilt caused by fusarium oxysporum f. sp lycopersici (fol) is one of the main diseases affecting tomatoes. the bhrs 2,3 genotype of tomato is, however, resistant to this disease. a proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. protein was extracted and separated by two-dimensional electrophoresis followed by staining with coomassie brilliant blue. the proteins were ide ...201728549197
transcriptomic dissection reveals wide spread differential expression in chickpea during early time points of fusarium oxysporum f. sp. ciceri race 1 attack.plants' reaction to underground microorganisms is complex as sessile nature of plants compels them to prioritize their responses to diverse microorganisms both pathogenic and symbiotic. roots of important crops are directly exposed to diverse microorganisms, but investigations involving root pathogens are significantly less. thus, more studies involving root pathogens and their target crops are necessitated to enrich the understanding of underground interactions. present study reported the molec ...201728542579
characterization of silver nanoparticles produced by biosynthesis mediated by fusarium oxysporum under different processing conditions.the goal of this study was the biosynthesis of silver nanoparticles (snps) mediated by the fungus fusarium oxysporum, as well as the characterization of these nanoparticles including evaluation of the particles size and stability under different processing conditions. the results showed that the biosynthesis produced silver nanoparticles having a mean size of 34 nm and zeta potential values below -30 mv at the conditions used, characterizing the nanoparticles as being stable in suspension. ultra ...201728540439
detoxification of fusaric acid by the soil microbe mucor rouxii.fusarium oxysporum f. sp. vasinfectum race 4 (vcg0114), which causes root rot and wilt of cotton (gossypium hirsutum and g. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the san joaquin valley of california. this pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of f. oxysporum f. sp. vasinfectum (fov) that are indigenous to the united states. fusaric a ...201728538103
wble2 transcription factor in streptomyces griseus s4-7 plays an important role in plant protection.streptomyces griseus s4-7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for fusarium wilt suppressive soils. s. griseus s4-7 shows specific and pronounced antifungal activity against fusarium oxysporum f. sp. fragariae. in the streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wble2 function as a regulator has emerged as a new group in whi transcription ...201728523731
non-edible oil cakes as a novel substrate for dpa production and augmenting biocontrol activity of paecilomyces variotii.the present study investigated the use of waste non-edible oil cakes (jatropha, karanja, neem, and mahua) as a substrate for the growth of paecilomyces variotii and dipicolinic acid (dpa) production. previous researches proved the efficacy of dpa in suppressing certain pathogens that are deleterious to the plants in the rhizosphere. dpa production was statistical optimized by amending non-edible oil cakes in growing media as nitrogen and sugars (dextrose, glucose, and lactose) as carbon source. ...201728512455
purification and site-directed mutagenesis of linoleate 9s-dioxygenase-allene oxide synthase of fusarium oxysporum confirms the oxygenation mechanism.plants and fungi form jasmonic acid from α-linolenic acid. the first two steps of biosynthesis in plants occur by sequential transformation by 13s-lipoxygenase and allene oxide synthase (aos). the biosynthesis in fungi may follow this classical scheme, but the only fungal aos discovered so far are cytochromes p450 (cyp) fused to 8- and 9-dioxygenases (dox). in the present report, we purified recombinant 9s-dox-aos of fusarium oxysporum from cell lysate by cobalt affinity chromatography to near h ...201728502466
effectiveness of a full-scale horizontal slow sand filter for controlling phytopathogens in recirculating hydroponics: from microbial isolation to full microbiome assessment.the microbial disinfestation efficiency of an innovative horizontal-flow slow sand filter (hssf) for treating nutrient solution spent from an experimental closed-loop nursery was evaluated by means of a combination of culture-dependent and independent molecular techniques. a dense inoculum of the fungal plant pathogen fusarium oxysporum f.sp. lycopersici was applied in the fertigation system (10(6) cells per ml). indigenous and introduced populations of eubacteria and fungi were assessed in the ...201728499226
fusarium oxysporum and the fusarium wilt syndrome.the fusarium oxysporum species complex (fosc) comprises a multitude of strains that cause vascular wilt diseases of economically important crops throughout the world. although sexual reproduction is unknown in the fosc, horizontal gene transfer may contribute to the observed diversity in pathogenic strains. development of disease in a susceptible crop requires f. oxysporum to advance through a series of transitions, beginning with spore germination and culminating with establishment of a systemi ...201728489498
the endochitinase vdech from verticillium dahliae inhibits spore germination and activates plant defense responses.chitinases function in the digestion of chitin molecules, which are present principally in insects and fungi. in plants, chitinase genes play important roles in defense, and their expression can be triggered in response to both biotic and abiotic stresses. in this study, we cloned and characterized an endochitinase (vdech) from verticillium dahliae, strain vd080. the vdech coding region consists of 1845bp with two exons and one 54bp intron, encoding a 615 amino acid protein with the predicted mo ...201728483050
comparative transcriptomic analysis of race 1 and race 4 of fusarium oxysporum f. sp. cubense induced with different carbon sources.the fungal pathogen fusarium oxysporum f. sp. cubense causes fusarium wilt, one of the most destructive diseases in banana and plantain cultivars. pathogenic race 1 attacks the "gros michel" banana cultivar, and race 4 is pathogenic to the cavendish banana cultivar and those cultivars that are susceptible to foc1. to understand the divergence in gene expression modules between the two races during degradation of the host cell wall, we performed rna sequencing to compare the genome-wide transcrip ...201728468818
genome-wide analysis of autophagy-related genes in banana highlights maatg8s in cell death and autophagy in immune response to fusarium wilt.maatg8s play important roles in hypersensitive-like cell death and immune response, and autophagy is essential for disease resistance against foc in banana. autophagy is responsible for the degradation of damaged cytoplasmic constituents in the lysosomes or vacuoles. although the effects of autophagy have been extensively revealed in model plants, the possible roles of autophagy-related gene in banana remain unknown. in this study, 32 maatgs were identified in the draft genome, and the profiles ...201728451821
mediator18 and mediator20 confer susceptibility to fusarium oxysporum in arabidopsis thaliana.the conserved protein complex known as mediator conveys transcriptional signals by acting as an intermediary between transcription factors and rna polymerase ii. as a result, mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. in this report we identify the head domain subunits mediator18 and mediator20 as important susceptibility factors for fusarium oxysporum infection in arabidopsis thaliana. mutants of med18 and med20 display down- ...201728441405
interdigital intertrigo due to fusarium oxysporum.fusariosis is a fungal infection often involving the skin. various species can cause local, focally invasive, or disseminated infections. the routes of entry for fusarium species include the respiratory tract, gastrointestinal tract, toe nails, trauma to the skin, and indwelling central venous catheter.201628681012
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