Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| skin and subcutaneous mycoses in tilapia (oreochromis niloticus) caused by fusarium oxysporum in coinfection with aeromonas hydrophila. | subcutaneous mycoses in freshwater fish are rare infections usually caused by oomycetes of the genus saprolegnia and some filamentous fungi. to date, fusarium infections in farmed fish have only been described in marine fish. here, we report the presence of fusarium oxysporum in subcutaneous lesions of nile tilapia (oreochromis niloticus). histopathologic evaluation revealed granuloma formation with fungal structures, and the identity of the etiological agent was demonstrated by morphological an ... | 2015 | 26155462 |
| ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease. | the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ... | 2015 | 26417353 |
| combining uv-c treatment with biocontrol yeast to control postharvest decay of melon. | significant losses in harvested melon can be directly attributable to decay fungi. in the present study, the use of uv-c treatment combined with biocontrol yeast, pichia cecembensis, was evaluated for their ability to control postharvest decay of melon fruits after they were artificially inoculated with fusarium oxysporum and alternaria alternata. natural infection of fruit was also assessed. as a stand-alone treatment, uv-c or p. cecembensis significantly reduced fusarium rot and alternaria rot ... | 2015 | 25976334 |
| [antifungal effect of phenolic and carotenoids extracts from chiltepin (capsicum annum var. glabriusculum) on alternaria alternata and fusarium oxysporum]. | the effect of phenolic and carotenoid extracts from chiltepin fruits on mycelial growth and the inhibition of conidial germination of alternaria alternata and fusarium oxysporum were investigated in the present work. phenolic extracts inhibited mycelial growth of a.alternata by 38.46%, and significantly reduced conidial germination on the fifth day after treatment to 92% in relation to control. no significant changes were observed in the inhibition of mycelial growth in fusarium oxysporum; howev ... | 2015 | 25705046 |
| antifungal activity of violacein purified from a novel strain of chromobacterium sp. niist (mtcc 5522). | a novel strain of chromobacterium sp. niist (mtcc 5522) producing high level of purple blue bioactive compound violacein was isolated from clay mine acidic sediment. during 24 h aerobic incubation in modified luria bertani medium, around 0.6 g crude violacein was produced per gram of dry weight biomass. an inexpensive method for preparing crystalline, pure violacein from crude pigment was developed (12.8 mg violacein/l) and the pure compound was characterized by different spectrometric methods. ... | 2015 | 26428920 |
| characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents. | a plant growth-promoting bacterial strain, pm 105, isolated from a tea plantation soil from the north eastern region of india was identified as pseudomonas aeruginosa through classical and 16s ribosomal dna (rdna) gene sequencing. further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus tubingensis, candida albicans, colletotrichum gloeospori ... | 2015 | 25832181 |
| diversity of food-borne bacillus volatile compounds and influence on fungal growth. | to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition. | 2015 | 25989039 |
| [screening probiotic endophytic bacteria from medicinal plant flex cornuta and the phytopathogen-inhibiting effect]. | culturable endophytic bacteria were isolated from medicinal plant ilex cornuta by plate-spreading method, strains with strong inhibitory effect on phytopathogen were screened by confrontation culture and fermentation filtrate culture methods, and the morphological changes of phytopathogen hyphae treated with endophytic bacteria were examined by microscopy and micrograph. their phylogenetic relationships were determined by homology analysis of the 16s rdna sequences of pcr products and the taxono ... | 2015 | 26571677 |
| in vitro assessment of extracts of the lingzhi or reishi medicinal mushroom, ganoderma lucidum (higher basidiomycetes) against different plant pathogenic fungi. | five isolates of the lingzhi or reishi medicinal mushroom ganoderma lucidum (gl-1, gl-2, gl-3, gl-4, gl-5) were collected from different locations within and surrounding lahore, pakistan, to study the antifungal potential of their bioactive compounds. after studying morphology, different concentrations of the extracts were prepared in methanol and water using a soxhlet extractor. different cultures of fungal pathogens were acquired from the first fungal culture bank of pakistan, university of th ... | 2015 | 25954966 |
| identification and characterization of an antifungal protein, afafpr9, produced by marine-derived aspergillus fumigatus r9. | a fungal strain, r9, was isolated from the south atlantic sediment sample and identified as aspergillus fumigatus. an antifungal protein, afafpr9, was purified from the culture supernatant of aspergillus fumigatus r9. afafpr9 was identified to be restrictocin, which is a member of the ribosome-inactivating proteins (rips), by maldi-tof-tof-ms. afafpr9 displayed antifungal activity against plant pathogenic fusarium oxysporum, alternaria longipes, colletotrichum gloeosporioides, paecilomyces vario ... | 2015 | 25394604 |
| fungal endophyte diversity and bioactivity in the indian medicinal plant ocimum sanctum linn. | endophytic mycopopulation isolated from india's queen of herbs tulsi (ocimum sanctum) were explored and investigated for their diversity and antiphytopathogenic activity against widespread plant pathogens botrytis cinerea, sclerotinia sclerotiorum, rhizoctonia solani and fusarium oxysporum. 90 fungal isolates, representing 17 genera were recovered from 313 disease-free and surface sterilised plant segments (leaf and stem tissues) from three different geographic locations (delhi, hyderabad and mu ... | 2015 | 26529087 |
| antimicrobial and inhibition on heat-induced protein denaturation of constituents isolated from polygonatum verticillatum rhizomes. | this study was designed to assess the susceptibility of various microorganisms and inhibition on heat-induced protein denaturation against diosgenin and santonin, isolated from polygonatum verticillatum rhizomes. both diosgenin and santonin showed significant zone of inhibition when studied against various gram-positive (bacillus subtilis, bacillus cereus, staphylococcus aureus and staphylococcus epidermidis) and gram-negative bacteria (escherichia coli and salmonella typhi). in antifungal assay ... | 2015 | 25553804 |
| 2,4-di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated lactococcus sp. | the volatile organic compound 2,4-di-tert-butyl phenol (2,4 dtbp) was purified from the cell free supernatant of a newly isolated lactococcus sp. by solvent extraction and chromatographic techniques. molecular characterization of the compound by esi-ms, (1)h nmr and ftir analysis revealed the structure, c14h22o. fungicidal activity was demonstrated against aspergillus niger, fusarium oxysporum and penicillium chrysogenum by disc diffusion assay. among the cell lines tested for cytotoxicity of th ... | 2015 | 26164257 |
| detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of fusarium crown and root rot disease of tomato. | to detect and characterize broad-spectrum antipathogen activity of indigenous bacterial isolates obtained from potato soil and soya bean leaves for their potential to be developed as biofungicides to control soilborne diseases such as fusarium crown and root rot of tomato (fcrr) caused by fusarium oxysporum f. sp. radicis-lycopersici (forl). | 2015 | 25512025 |
| molecular characterization of a penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins. | we previously described an endo-acting rhamnogalacturonan (rg) lyase, termed pcrgl4a, of penicillium chrysogenum 31b. here, we describe a second rg lyase, called pcrglx. we determined the cdna sequence of the pcrglx gene, which encodes pcrglx. based on analyses using a blast search and a conserved domain search, pcrglx was found to be structurally distinct from known rg lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of nectria haematoco ... | 2015 | 25921806 |
| antagonistic activities of novel peptides from bacillus amyloliquefaciens pt14 against fusarium solani and fusarium oxysporum. | bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. this paper reports on the antifungal activity of novel peptides isolated from bacillus amyloliquefaciens pt14. reverse-phase high-performance liquid chromatography revealed that b. amyloliquefaciens pt14 produces five peptides (pt14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. in particular, pt14-3 and pt14-4a showed broa ... | 2015 | 26496638 |
| purification and identification of two antifungal cyclic peptides produced by bacillus amyloliquefaciens l-h15. | bacillus amyloliquefaciens l-h15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress fusarium oxysporum and other soil-borne fungal plant pathogens. two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. the two compounds were identified by tandem q-tof mass spectroscopy as c15 iturin a (1) and a novel cyclic peptide with a molecular weight of 852.4 da (2). both compounds showed good inhibitory activities ... | 2015 | 26123083 |
| chemical composition and antifungal activity of aaronsohnia pubescens essential oil from algeria. | in the present study, the chemical composition of the essential oil obtained from the aerial parts of aaronsohnia pubescens (desf.) k.bremer & humphries (asteraceae), a chamomile-like medicinal plant, was studied. using both gc-ms and gc-fid methods, 58 volatile compounds could be identified representing 96.6% of the total essential oil composition. the main compounds in the essential oil were monoterpene hydrocarbons such as (z)-β-ocimene (53.8%), myrcene (15.2%) and α-pinene (7.7%). moreover, ... | 2015 | 25920238 |
| studies on antimicrobial effects of four ligands and their transition metal complexes with 8-mercaptoquinoline and pyridine terminal groups. | four types of ligands (q1-q4) and their complexes (1-36) with transition metal ions have been synthesized, in which two new complexes (15 and 20) have been prepared and tested. in vitro antimicrobial activities of the ligands and their complexes were investigated against a representative panel of strains including two gram positive bacteria (sarcina ureae, staphylococcus aureus), two gram negative bacteria (escherichia coli, pseudomonas aeruginosa) and three fungi (aspergillus niger, saccharomyc ... | 2015 | 25791454 |
| isolation and expression of enolase gene in fusarium oxysporum f. sp. lycopersici. | fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. enolase genes have been isolated from bacteria and fungi, among other organisms. in this research, a large portion of the enolase, eno, gene sequence was isolated from f. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 ... | 2015 | 25349089 |
| possible mechanism of antifungal phenazine-1-carboxamide from pseudomonas sp. against dimorphic fungi benjaminiella poitrasii and human pathogen candida albicans. | investigation of antifungal mechanism of phenazine 1-carboxamide (pc) produced by a pseudomonas strain mcc2142. | 2015 | 25348290 |
| minos as a novel tc1/mariner-type transposable element for functional genomic analysis in aspergillus nidulans. | transposons constitute powerful genetic tools for gene inactivation, exon or promoter trapping and genome analyses. the minos element from drosophila hydei, a tc1/mariner-like transposon, has proved as a very efficient tool for heterologous transposition in several metazoa. in filamentous fungi, only a handful of fungal-specific transposable elements have been exploited as genetic tools, with the impala tc1/mariner element from fusarium oxysporum being the most successful. here, we developed a t ... | 2015 | 26021704 |
| in-vitro assessment of the probiotic potential of lactobacillus plantarum kcc-24 isolated from italian rye-grass (lolium multiflorum) forage. | the aim of the present study was to determine the probiotic potential of the lactic acid bacteria lactobacillus plantarum kcc-24 (l. plantarum kcc-24), that was isolated and characterized from italian ryegrass (lolium multiflorum) forage. the following experiments were performed to assess the probiotic characteristics such as antifungal activity, antibiotic susceptibility, resistance to low ph, stimulated gastric juice and bile salts, proteolytic activity, auto-aggregation, cell surface hydropho ... | 2015 | 25576238 |
| probiotic potential of lactobacillus strains with antifungal activity isolated from animal manure. | the aim of the study was to isolate and characterize the lactic acid bacteria (lab) from animal manure. among the thirty lab strains, four strains, namely, kcc-25, kcc-26, kcc-27, and kcc-28, showed good cell growth and antifungal activity and were selected for further characterization. biochemical and physiology properties of strains confirmed that the strains are related to the lactobacillus sp.; further, the 16s rrna sequencing confirmed 99.99% sequence similarity towards lactobacillus planta ... | 2015 | 26167534 |
| antimicrobial activity and chemical composition of the essential oils of portuguese foeniculum vulgare fruits. | the aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of portugal. composition of essential oils was established by gas chromatography-flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms) analysis. the obtained yields of the essential oils were found to vary greatly in the range ... | 2015 | 25973507 |
| hormonal regulation of gummosis and composition of gums from bulbs of hyacinth (hyacinthus orientalis). | hyacinth (hyacinthus orientalis) bulbs infected by fusarium oxysporum showed the symptoms of gummosis. the purpose of this study was to clarify the hormonal regulation of gummosis and composition of gums from hyacinth bulbs. the application of ethephon (2-chloroethylphosphonic acid), an ethylene-releasing compound, at 2% (w/w, in lanolin) induced gummosis in hyacinth bulbs. methyl jasmonate (ja-me) at 1.5% (w/w, in lanolin) induced gummosis as well. simultaneous application of ja-me and ethephon ... | 2015 | 25462960 |
| response of nbs encoding resistance genes linked to both heat and fungal stress in brassica oleracea. | environmental stresses, including both abiotic and biotic stresses, cause considerable yield loss in crops and can significantly affect their development. under field conditions, crops are exposed to a variety of concurrent stresses. among abiotic and biotic stresses, heat and fusarium oxysporum, are the most important factors affecting development and yield productivity of brassica oleracea. genes encoding the nucleotide-binding site (nbs) motif are known to be related to responses to abiotic a ... | 2015 | 25461701 |
| arsenic speciation transformation and arsenite influx and efflux across the cell membrane of fungi investigated using hplc-hg-afs and in-situ xanes. | microorganisms dominated speciation of arsenic (as) play an important role in the biogeochemical cycling of as. in the study, species transformation of arsenite [as(iii)] and as(iii) influx and efflux across the cell membranes of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 cells were studied using a cellular lysis plus chromatographic separation method and further the in-situ x-ray absorption near edge structure (xanes) analysis. the results in ... | 2015 | 25460757 |
| mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in fusarium oxysporum f. sp. cubense. | fusarium oxysporum f. sp. cubense (foc) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. however, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. in this study, we showed that null mutation of three mitogen-activated protein (map) kinase genes, designated as foslt2, fomkk2 an ... | 2015 | 25849862 |
| [differential gene expression in incompatible interaction between lilium regale wilson and fusarium oxysporum f. sp. lilii revealed by combined ssh and microarray analysis]. | fusarium wilt, caused by a soilborne pathogen fusarium oxysporum f. sp. lilii, is the major disease of lily (lilium l.). in order to isolate the genes differentially expressed in a resistant reaction to f. oxysporum in l. regale wilson, a cdna library was constructed with l. regale root during f. oxysporum infection using the suppression subtractive hybridization (ssh), and a total of 585 unique expressed sequence tags (ests) were obtained. furthermore, the gene expression profiles in the incomp ... | 2015 | 25845232 |
| preparation and characterization of a novel extracellular polysaccharide with antioxidant activity, from the mangrove-associated fungus fusarium oxysporum. | marine fungi are recognized as an abundant source of extracellular polysaccharides with novel structures. mangrove fungi constitute the second largest ecological group of the marine fungi, and many of them are new or inadequately described species and may produce extracellular polysaccharides with novel functions and structures that could be explored as a source of useful polymers. the mangrove-associated fungus fusarium oxysporum produces an extracellular polysaccharide, fw-1, when grown in pot ... | 2015 | 25627692 |
| fubt, a putative mfs transporter, promotes secretion of fusaric acid in the cotton pathogen fusarium oxysporum f. sp. vasinfectum. | fusaric acid (fa) is a key component in virulence and symptom development in cotton during infection by fusarium oxysporum. a putative major facilitator superfamily (mfs) transporter gene was identified downstream of the polyketide synthase gene responsible for the biosynthesis of fa in a region previously believed to be unrelated to the known fa gene cluster. disruption of the transporter gene, designated fubt, resulted in loss of fa secretion, decrease in fa production and a decrease in resist ... | 2015 | 25627440 |
| a novel ionic liquid-tolerant fusarium oxysporum bn secreting ionic liquid-stable cellulase: consolidated bioprocessing of pretreated lignocellulose containing residual ionic liquid. | in this study, microbial communities from chemicals polluted microhabitats were cultured with the addition of imidazolium-based ionic liquid (il) to enrich for il-tolerant microbes. a strain of fusarium oxysporum bn producing cellulase from these enrichments was capable of growing in 10% (w/v) 1-ethyl-3-methylimidazolium phosphinate, much higher than the normal il concentrations in the lignocellulose regenerated from ils. cellulase secreted by the strain showed high resistance to ils based on ph ... | 2015 | 25625459 |
| activity of haliscosamine against fusarium oxysporum f.sp. melonis: in vitro and in vivo analysis. | marine sponges are a potential source of new molecules with diverse biological activities. we have previously isolated a sphingosine derivative, (9z)-2-amino-docos-9-ene-1,3,13,14-tetraol (haliscosamine) from the moroccan sea sponge haliclona viscosa. the aim of this study was to test haliscosamine in vitro and in vivo for its antifungal activity against fusarium oxysporum f.sp. melonis causing fusarium wilt of melon. overall, in vitro test showed that haliscosamine has a similar effect as desog ... | 2015 | 25625038 |
| characterization and stress-induced expression analysis of alfin-like transcription factors in brassica rapa. | the alfin-like (al) transcription factors (tfs) family is involved in many developmental processes, including the growth and development of roots, root hair elongation, meristem development, etc. however, stress resistance-related function and the regulatory mechanism of these tfs have yet to be elucidated. this study identified 15 brassica rapa al (bral) tfs from brad database, analyzed the sequences and profiled their expression first time in response to fusarium oxysporum f. sp. conglutinans ... | 2015 | 25618423 |
| synthesis and in vitro antifungal efficacy of cu-chitosan nanoparticles against pathogenic fungi of tomato. | cu-chitosan nanoparticles were synthesized and evaluated for their growth promotory and antifungal efficacy in tomato (solanum lycopersicum mill). physico-chemical characterization of the developed cu-chitosan nanoparticles was carried out by dls, ftir, tem, sem-eds and aas. the study highlighted the stability and porous nature of cu-chitosan nanoparticles. laboratory synthesized nanoparticles showed substantial growth promotory effect on tomato seed germination, seedling length, fresh and dry w ... | 2015 | 25617841 |
| water balance altered in cucumber plants infected with fusarium oxysporum f. sp. cucumerinum. | fusarium wilt is caused by the infection and growth of the fungus fusarium oxysporum in the xylem of host plants. the physiological responses of cucumbers that are infected with fusarium oxysporum f. sp. cucumerinum (foc) was studied in pot and hydroponic experiments in a greenhouse. the results showed that although water absorption and stem hydraulic conductance decreased markedly in infected plants, large amounts of red ink accumulated in the leaves of infected cucumber plants. the transpirati ... | 2015 | 25579504 |
| autophagy contributes to regulation of nuclear dynamics during vegetative growth and hyphal fusion in fusarium oxysporum. | in the fungal pathogen fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. here we examined the role of autophagy in fusion-induced nuclear degradation. a search of the f. oxysporum genome database for autophagy pathway components identified putative orthologs of 16 core autophagy-related (atg) genes in yeast, including the ubiquitin-like protein at ... | 2015 | 25560310 |
| [repeating the platelia™ aspergillus test in samples positive for serum galactomannan: is it necessary?]. | the manufacturers of the platelia™ aspergillus test recommend retesting every sample with a positive result to galactomannan detection. | 2015 | 25556050 |
| pre-planting treatments with phosphite-based products against different foliar and soil-borne pathogens of vegetable crops. | fifteen experimental trials were carried out under greenhouse conditions to evaluate the efficacy of preventative treatments based on phosphite salts on the following pathosystems: tomato/phytophthora nicotianae, zucchini/p. capsici, lettuce/fusarium oxysporum f.sp. iactucae, rocket/fusarium oxysporum f. sp. raphani, wild rocket/plectosphaerella cucumerina and basii/peronospora belbahrii. the possible use of phosphite salts in nursery cultivation systems is considered in comparison with chemical ... | 2015 | 27141741 |
| biochemical defenses induced by mycorrhizae fungi glomus mosseae in controlling strawberry fusarium wilt. | the effect of vam on reducing wilt caused by fusarium oxysporum schlecht. f.sp. fragariae winks et williams (fo) infection in strawberry and the possible mechanisms involved were investigated. two key substance involved in disease defenses, lignin and hydroxyproline-rich glycoprotein were induced and formed in the cell wall of strawberry root, and the peak content of lignin and hydroxyproline-rich glycoprotein occurred on the 25(th) day (149.52mg/g) and on the 15(th) day (10.08 mg/g). the activi ... | 2015 | 26998177 |
| phenyl derivative of pyranocoumarin precludes fusarium oxysporum f.sp. lycopersici infection in lycopersicon esculentum via induction of enzymes of the phenylpropanoid pathway. | binding of phenyl derivative of pyranocoumarin (pdp) modulated activity of fungal endopolygalacturonase in silico. induced fit docking study of pdp with endopolygalacturonase (1hg8) showed a bifurcated hydrogen bond interaction with the protein at lys 244 with a docking score of -3.6 and glide energy of -37.30 kcal/mol. docking with endopolygalacturonase ii (1czf) resulted hydrogen bond formation with lys 258 with a docking score of -2.3 and glide energy of -30.42 kcal/mol. it was hypothesized t ... | 2015 | 25374140 |
| analyses of fusarium wilt race 3 resistance in upland cotton (gossypium hirsutum l.). | fusarium wilt [fusarium oxysporum f.sp. vasinfectum (fov) atk. sny & hans] represents a serious threat to cotton (gossypium spp.) production. for the last few decades, the fov pathogen has become a significant problem in uzbekistan causing severe wilt disease and yield losses of g. hirsutum l. cultivars. we present the first genetic analyses of fov race 3 resistance on uzbek cotton germplasm with a series of field and greenhouse artificial inoculation-evaluations and inheritance studies. the fie ... | 2015 | 25896369 |
| synthesis, insecticidal, and fungicidal screening of some new synthetic quinoline derivatives. | this paper describes the synthesis of a series of quinolines graphted with hydrazones, pyrazoles, pyridazine, phthalazine, triazepinone, semicarbazide, and thiomorpholide moieties and four metal complexes. these derivatives were screened against fusarium oxysporum and the red palm weevil (rpw) rhynchophorus ferrugineus oliver (coleopteran: curculionidae) as palm pathogens. only chlorinated quinolines were active against these organisms with hydrazones being good fungicides, while those modified ... | 2015 | 25895344 |
| n2o production, a widespread trait in fungi. | n2o is a powerful greenhouse gas contributing both to global warming and ozone depletion. while fungi have been identified as a putative source of n2o, little is known about their production of this greenhouse gas. here we investigated the n2o-producing ability of a collection of 207 fungal isolates. seventy strains producing n2o in pure culture were identified. they were mostly species from the order hypocreales order-particularly fusarium oxysporum and trichoderma spp.-and to a lesser extent s ... | 2015 | 25894103 |
| ethanol effect on metabolic activity of the ethalogenic fungus fusarium oxysporum. | fusarium oxysporum is a filamentous fungus which has attracted a lot of scientific interest not only due to its ability to produce a variety of lignocellulolytic enzymes, but also because it is able to ferment both hexoses and pentoses to ethanol. although this fungus has been studied a lot as a cell factory, regarding applications for the production of bioethanol and other high added value products, no systematic study has been performed concerning its ethanol tolerance levels. | 2015 | 25887038 |
| development of loop-mediated isothermal amplification (lamp) assay for rapid detection of fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpea. | fusarium oxysporum f. sp. ciceris (foc), the causal agent of fusarium wilt is a devastating pathogen of chickpea. in chickpea, various soil borne pathogens produce (s) similar symptoms, therefore cannot be distinguished easily at field level. there is real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of fusarium wilt outbreaks. | 2015 | 25886622 |
| emergence of fusarioses in a university hospital in turkey during a 20-year period. | fusarium species have started appearing increasingly as the main cause of infections, particularly in immunocompromised patients. in this study, we aimed to present the first epidemiological data from turkey, analyze fusariosis cases that have been monitored in a university hospital during the past 20 years, identify the responsible fusarium species, and determine antifungal susceptibilities. a total of 47 cases of fusariosis was included in the study. fusarium isolates were identified by multil ... | 2015 | 25994844 |
| in vitro study of the growth, development and pathogenicity responses of fusarium oxysporum to phthalic acid, an autotoxin from lanzhou lily. | continuous monoculture of lanzhou lily (lilium davidii var. unicolor cotton) results in frequent incidence of fusarium wilt caused by fusarium oxysporum. phthalic acid (pa), a principal autotoxin from root exudates of lanzhou lily, is involved in soil sickness by inducing autotoxicity. the aim of this study was to evaluate the direct allelopathic effects of pa on the growth, development and pathogenicity of f. oxysporum in vitro based on an ecologically relevant soil concentration. the results s ... | 2015 | 25994089 |
| cutin from watermelon peels: a novel inducer for cutinase production and its physicochemical characterization. | cutin a polymeric biological macromolecule made up of esterified polyacids which acts as an inducer of cutinase. the present work was based on screening and characterization of newer cutin source from the agro industrial wastes as inducers of cutinase production by fusarium oxysporum mtcc 2480. cutin isolated from peels of multi green colored watermelon (wmc) yielded 6.77 u/ml as compared to that 9.64 u/ml of cutinase using apple cutin. the ftir and (13)c cp-mas solid state nmr studies indicated ... | 2015 | 25987459 |
| pharmacogenomic and molecular docking studies on the cytotoxicity of the natural steroid wortmannin against multidrug-resistant tumor cells. | wortmannin is a cytotoxic compound derived from the endophytic fungi fusarium oxysporum, penicillium wortmannii and penicillium funiculosum that occurs in many plants, including medicinal herbs. the rationale to develop novel anticancer drugs is the frequent development of tumor resistance to the existing antineoplasic agents. therefore, it is mandatory to analyze resistance mechanisms of novel drug candidates such as wortmannin as well to bring effective drugs into the clinic that have the pote ... | 2015 | 25636880 |
| the tomato i-3 gene: a novel gene for resistance to fusarium wilt disease. | plant resistance proteins provide race-specific immunity through the recognition of pathogen effectors. the resistance genes i, i-2 and i-3 have been incorporated into cultivated tomato (solanum lycopersicum) from wild tomato species to confer resistance against fusarium oxysporum f. sp. lycopersici (fol) races 1, 2 and 3, respectively. although the fol effectors corresponding to these resistance genes have all been identified, only the i-2 resistance gene has been isolated from tomato. to isola ... | 2015 | 25740416 |
| comparison of the physiological characteristics of transgenic insect-resistant cotton and conventional lines. | the introduction of transgenic insect-resistant cotton into agricultural ecosystems has raised concerns regarding its ecological effects. many studies have been conducted to compare the differences in characteristics between transgenic cotton and conventional counterparts. however, few studies have focused on the different responses of transgenic cotton to stress conditions, especially to the challenges of pathogens. the aim of this work is to determine the extent of variation in physiological c ... | 2015 | 25737015 |
| correction: genome and transcriptome analysis of the fungal pathogen fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. | 2015 | 25629969 | |
| fusarium diversity in soil using a specific molecular approach and a cultural approach. | fusarium species are ubiquitous in soil. they cause plant and human diseases and can produce mycotoxins. surveys of fusarium species diversity in environmental samples usually rely on laborious culture-based methods. in the present study, we have developed a molecular method to analyze fusarium diversity directly from soil dna. we designed primers targeting the translation elongation factor 1-alpha (ef-1α) gene and demonstrated their specificity toward fusarium using a large collection of fungi. ... | 2015 | 25655778 |
| down-regulation of fusarium oxysporum endogenous genes by host-delivered rna interference enhances disease resistance. | fusarium oxysporum is a devastating pathogen causing extensive yield losses in a variety of crops and development of sustainable, environmentally friendly methods to improve crop resistance is crucial. we have used host-delivered rna interference (hd-rnai) technology to partially silence three different genes (fow2, frp1, and opr) in the hemi-biotrophic fungus f. oxysporum f. sp. conglutinans. expression of double stranded rna (dsrna) molecules targeting fungal pathogen genes was achieved in a n ... | 2015 | 25654075 |
| distribution of keratinophilic fungi in soil across tunisia: a descriptive study and review of the literature. | data on the frequency and distribution of keratinophilic fungi in soil of tunisia are scanty. the present survey aimed to describe the distribution of keratinophilic fungi in soils collected in tunisia. keratinophilic fungi were isolated using vanbreuseghem's hair-baiting technique from 354 soil samples collected in 15 governorates of tunisia and identified according to their morphology with further dna and maldi-tof analysis when necessary. keratinophilic fungi were isolated from 46.3 % of the ... | 2015 | 25690159 |
| fluorescence assisted selection of transformants (fast): using flow cytometry to select fungal transformants. | the availability of drug resistance markers for fungal transformation is often a limiting factor in both fungal genetics research and industrial applications. we describe a new technique using flow cytometry to select fungal transformants using well-known fluorescent proteins as markers for transformation. this new technique, fluorescence-assisted selection of transformants (fast), was used for a transformation of fusarium oxysporum with gfp as a marker targeted at a specific site on chromosome ... | 2015 | 25687933 |
| effect of biosolarisation on the microbial populations of substrates infested with fusarium oxysporum by pcr-dgge. | biosolarisation consists of combining solarisation and organic matter application for controlling soilborne pathogens. the effects of this control strategy on the microbial community is almost unknown and needs to be investigated with molecular tools. the aim of the research was to investigate how biosolarisation can affect the structure of the microbial populations evaluated by a culture independent method using dgge of pcr-amplified 18s-its genes-coding fragments from dna extracted directly fr ... | 2015 | 27141761 |
| characterization and biocontrol potent of streptomyces sp. isolated from the rhizosphere of ononis angustissima lam. | a total of 40 actinomycetes isolated from rhizosphere soils of ononis angustissima lam. were in vitro tested for their antagonism against deferent pathogenic microorganisms by streak assay. among the isolates, four (21, 2a26, 1b10 and 2c34) present a potent antagonism against both pathogenic bacteria and fungi, they were selected, identified by 16s rdna sequence analysis and phenotypic properties, and tested for their antimicrobial activity as well as their biocontrol potential against chickpea ... | 2015 | 27141751 |
| the application of arabidopsis thaliana in studying tripartite interactions among plants, beneficial fungal endophytes and biotrophic plant-parasitic nematodes. | the research demonstrated that arabidopsis can be used as a model system for studying plant-nematode-endophyte tripartite interactions; thus, opening new possibilities for further characterizing the molecular mechanisms behind these interactions. arabidopsis has been established as an important model system for studying plant biology and plant-microbe interactions. we show that this plant can also be used for studying the tripartite interactions among plants, the root-knot nematode meloidogyne i ... | 2015 | 25548000 |
| comparative study of the bioconversion process using r-(+)- and s-(-)-limonene as substrates for fusarium oxysporum 152b. | this study compared the bioconversion process of s-(-)-limonene into limonene-1,2-diol with the already established biotransformation of r-(+)-limonene into α-terpineol using the same biocatalyst in both processes, fusarium oxysporum 152b. the bioconversion of the s-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. when submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limone ... | 2015 | 25529726 |
| shifts in soil chemical properties and bacterial communities responding to biotransformed dry olive residue used as organic amendment. | dry olive residue (dor) is a waste product derived from olive oil extraction and has been proposed as an organic amendment. however, it has been demonstrated that a pre-treatment, such as its transformation by saprophytic fungi, is required before dor soil application. a greenhouse experiment was designed where 0 and 50 g kg(-1) of raw dor (dor), coriolopsis floccosa-transformed dor (cordor) and fusarium oxysporum-transformed dor (fusdor) were added to soil. analyses of the soil chemical propert ... | 2015 | 25501891 |
| roles of three fusarium oxysporum calcium ion (ca(2+)) channels in generating ca(2+) signatures and controlling growth. | spatial and temporal changes of cytoplasmic calcium ions ([ca(2+)]c), caused by external stimuli, are known as the ca(2+) signature and presumably control cellular and developmental responses. multiple types of ion channels, pumps, and transporters on plasma and organellar membranes modulate influx and efflux of ca(2+) to and from the extracellular environment and internal ca(2+) stores to form ca(2+) signatures. expression of a fluorescent protein-based ca(2+) probe, cameleon yc3.60, in fusariu ... | 2015 | 26162966 |
| fusarium wilt of banana. | banana (musa spp.) is one of the world's most important fruits. in 2011, 145 million metric tons, worth an estimated $44 billion, were produced in over 130 countries. fusarium wilt (also known as panama disease) is one of the most destructive diseases of this crop. it devastated the 'gros michel'-based export trades before the mid-1900s, and threatens the cavendish cultivars that were used to replace it; in total, the latter cultivars are now responsible for approximately 45% of all production. ... | 2015 | 26057187 |
| development of co-dominant scar markers linked to resistant gene against the fusarium oxysporum f. sp. radicis-lycopersici. | we developed highly reliable co-dominant scar markers linked to the frl gene. forl testing is difficult. the marker is expected to be quickly adapted for mas by tomato breeders. fusarium oxysporum f. sp. radicis-lycopersici causes fusarium crown and root rot (fcr), an economically important soil-borne disease of tomato. the resistance against fcr is conferred by a single dominant gene (frl) located on chromosome 9. the aim of this study was to develop molecular markers linked to the frl gene for ... | 2015 | 26037087 |
| focvel1 influences asexual production, filamentous growth, biofilm formation, and virulence in fusarium oxysporum f. sp. cucumerinum. | velvet genes play critical roles in the regulation of diverse cellular processes. in current study, we identified the gene focvel1, a homolog of fusarium graminearum vela, in the plant pathogenic fungus f. oxysporum f. sp. cucumerinum. this pathogen causes the destructive disease called cucumber fusarium wilt (cfw), which severely affects the production and marketing of this vegetable worldwide. transcript analyses revealed high expression of focvel1 during conidiophore development. disruption o ... | 2015 | 25999976 |
| molecular phylogeny and diversity of fusarium endophytes isolated from tomato stems. | plant tissues are a known habitat for two types of fusarium species: plant pathogens and endophytes. here, we investigated the molecular phylogeny and diversity of endophytic fusaria, because endophytes are not as well studied as pathogens. a total of 543 fusarium isolates were obtained from the inside of tomato stems cultivated in soils mainly obtained from agricultural fields. we then determined partial nucleotide sequences of the translation elongation factor-1 alpha (ef-1α) genes of the isol ... | 2015 | 26298015 |
| efficacy of fungal decolorization of a mixture of dyes belonging to different classes. | dyes are the most difficult constituents to remove by conventional biological wastewater treatment. colored wastewater is mainly eliminated by physical and chemical procedures, which are very expensive and have drawbacks. therefore, the advantage of using biological processes, such as the biotransformation of dyes, is that they may lead to complete mineralization or formation of less toxic products. to prove the possibility of using fungal processes for decolorization and other applications, the ... | 2015 | 26273256 |
| genome-wide analysis in three fusarium pathogens identifies rapidly evolving chromosomes and genes associated with pathogenicity. | pathogens and hosts are in an ongoing arms race and genes involved in host-pathogen interactions are likely to undergo diversifying selection. fusarium plant pathogens have evolved diverse infection strategies, but how they interact with their hosts in the biotrophic infection stage remains puzzling. to address this, we analyzed the genomes of three fusarium plant pathogens for genes that are under diversifying selection. we found a two-speed genome structure both on the chromosome and gene grou ... | 2015 | 25994930 |
| proteomic analysis of conidia germination in fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling fusarium wilt of banana. | conidial germination is a crucial step of the soilborne fungus fusarium oxysporum f. sp. cubense tropical race 4 (foc tr4), a most important lethal disease of banana. in this study, a total of 3659 proteins were identified by isobaric tags for relative and absolute quantitation (itraq)-based comparative proteomic approach, of which 1009 were differentially expressed during conidial germination of the fungus at 0, 3, 7, and 11 h. functional classification and bioinformatics analysis revealed that ... | 2015 | 26129952 |
| biosynthesized silver nanoparticles performing as biogenic sers-nanotags for investigation of c26 colon carcinoma cells. | in this work, two classes of silver nanoparticles (agnps) were biosynthesized with the goal to assess their reliability in vitro as surface-enhanced raman scattering (sers) nanotags. mycosynthesized silver nanoparticles (magnps) and phytosynthesized silver nanoparticles (pagnps) were produced through environmentally friendly procedures by reduction of silver nitrate with fusarium oxysporum cell filtrate and azadirachta indica extract, respectively. two cell lines, namely c26 murine colon carcino ... | 2015 | 26123850 |
| comparative proteomic analyses reveal that gnt2-mediated n-glycosylation affects cell wall glycans and protein content in fusarium oxysporum. | protein n-glycosylation is a ubiquitous post-translational modification that contributes to appropriate protein folding, stability, functionality and localization. n-glycosylation has been identified as an important process for morphogenesis and virulence in several fungal pathogens including fusarium oxysporum. here we conducted comparative chemical and proteome-based analyses to better understand the physiological changes associated with protein hypo-n-glycosylation in f. oxysporum n-glycosylt ... | 2015 | 26254006 |
| effect of vinegar residue compost amendments on cucumber growth and fusarium wilt. | fusarium wilt of cucumber caused by fusarium oxysporum f. sp. cucumerinum j. h. owen is one of the major destructive soilborne diseases and results in considerable yield losses. methyl bromide was once the most effective disease control method but has been confirmed as harmful to the environment. using suppressive media as biological controls to assist crop growth is becoming popular. in this study, fusarium wilt of cucumber was successfully controlled by a newly identified suppressive media: vi ... | 2015 | 26250808 |
| genetic analysis of verticillium wilt resistance in a backcross inbred line population and a meta-analysis of quantitative trait loci for disease resistance in cotton. | verticillium wilt (vw) and fusarium wilt (fw), caused by the soil-borne fungi verticillium dahliae and fusarium oxysporum f. sp. vasinfectum, respectively, are two most destructive diseases in cotton production worldwide. root-knot nematodes (meloidogyne incognita, rkn) and reniform nematodes (rotylenchulus reniformis, rn) cause the highest yield loss in the u.s. planting disease resistant cultivars is the most cost effective control method. numerous studies have reported mapping of quantitative ... | 2015 | 26239843 |
| compost and biochar alter mycorrhization, tomato root exudation, and development of fusarium oxysporum f. sp. lycopersici. | soil amendments like compost and biochar are known to affect soil properties, plant growth as well as soil borne plant pathogens. complex interactions based on microbial activity and abiotic characteristics are supposed to be responsible for suppressive properties of certain substrates, however, the specific mechanisms of action are still widely unknown. in the present study, the main focus was on the development of the soil borne pathogen, fusarium oxysporum f.sp. lycopersici (fol) in tomato (s ... | 2015 | 26217373 |
| first report on fusarium wilt of zucchini caused by fusarium oxysporum, in korea. | fusarium wilt of zucchini in jeonju, korea, was first noticed in may 2013. symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. infected plants eventually died during growth. based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rdna and translation elongation factor 1α), the fungus was identified as fusarium oxysporum. pathogenicity of a representative isolate was demonstrated via ... | 2015 | 26190927 |
| synthesis of a novel series of fluoroarene derivatives of artemisinin as potent antifungal and anticancer agent. | a series of new fluoroarene derivatives of artemisinin were prepared using suzuki and sonogashira cross-coupling reactions. an antifungal and antitumor activity was evaluated against opportunistic pathogen fusarium oxysporum and hela cancer cell line. all these derivatives have shown moderate to good activity. | 2015 | 26099535 |
| use of fusarium oxysporum schl cucumerinum transformed with green fluorescent protein to follow infection patterns in three cucurbit plants. | fusarium oxysporum schl is a soil-borne pathogen fungus, which could cause plant wilt diseases and caused great economic losses of agricultural production all over the world. however, the species of melon wilt diseases are too complex to breed stable disease-resistant melon strain. therefore, exploring the main specialized differentiation factors of cucurbit wilt pathogen is important for elucidating the pathogenesis. in this study, we constructed green fluorescent protein (gfp)-labeled fusarium ... | 2015 | 27617956 |
| comparative proteomics analyses of two races of fusarium oxysporum f. sp. conglutinans that differ in pathogenicity. | fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in a variety of plants. f. oxysporum f. sp. conglutinans (foc), which comprises two races, can cause wilt disease in cabbage. compared with race 1 (52557(-tm), r1), race 2 (58385(-tm), r2) exhibits much stronger pathogenicity. here, we provide the first proteome reference maps for foc mycelium and conidia and identify 145 proteins with different abundances among the two races. of these proteins, most of the hi ... | 2015 | 26333982 |
| adaptation of fusarium oxysporum and fusarium dimerum to the specific aquatic environment provided by the water systems of hospitals. | members of the fusarium group were recently detected in water distribution systems of several hospitals in the world. an epidemiological investigation was conducted over 2 years in hospital buildings in dijon and nancy (france) and in non-hospital buildings in dijon. the fungi were detected only within the water distribution systems of the hospital buildings and also, but at very low concentrations, in the urban water network of nancy. all fungi were identified as fusarium oxysporum species comp ... | 2015 | 25792434 |
| gene expression patterns and dynamics of the colonization of common bean (phaseolus vulgaris l.) by highly virulent and weakly virulent strains of fusarium oxysporum. | the dynamics of root and hypocotyl colonization, and the gene expression patterns of several fungal virulence factors and plant defense factors have been analyzed and compared in the interaction of two fusarium oxysporum f. sp. phaseoli strains displaying clear differences in virulence, with a susceptible common bean cultivar. the growth of the two strains on the root surface and the colonization of the root was quantitatively similar although the highly virulent (hv) strain was more efficient r ... | 2015 | 25883592 |
| fungal cytochrome p450 monooxygenases of fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered saccharomyces cerevisiae. | omega hydroxy fatty acids (ω-ohfas) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. however, the terminal oxygenation of saturated or unsaturated fatty acids for the synthesis of ω-ohfas is intricate to accomplish through chemocatalysis, due to the selectivity and controlled reactivity in c-h oxygenation reactions. cytochrome p450, the ubiquitous enzyme is capable of catalyzing the select ... | 2015 | 25880760 |
| priming of seeds with methyl jasmonate induced resistance to hemi-biotroph fusarium oxysporum f.sp. lycopersici in tomato via 12-oxo-phytodienoic acid, salicylic acid, and flavonol accumulation. | methyl jasmonate (meja) was tested by seed treatment for its ability to protect tomato seedlings against fusarium wilt caused by the soil-borne fungal pathogen fusarium oxysporum f.sp. lycopersici. isolated from solanum lycopersicon l. seeds, cv. beta fungus was identified as f. oxysporum f.sp. lycopersici race 3 fungus by using phytopathological and molecular methods. meja applied at 0.01, 0.1 and 1 mm reduced spore germination and mycelial growth in vitro. soaking of tomato seeds in meja solut ... | 2015 | 25867625 |
| glycogen catabolism, but not its biosynthesis, affects virulence of fusarium oxysporum on the plant host. | the role of glycogen metabolism was investigated in the fungal pathogen fusarium oxysporum. targeted inactivation was performed of genes responsible for glycogen biosynthesis: gnn1 encoding glycogenin, gls1 encoding glycogen synthase, and gbe1 encoding glycogen branching enzyme. moreover genes involved in glycogen catabolism were deleted: gph1 encoding glycogen phosphorylase and gdb1 encoding glycogen de-branching enzyme. glycogen reserves increased steadily during growth of the wild type strain ... | 2015 | 25865793 |
| understanding pea resistance mechanisms in response to fusarium oxysporum through proteomic analysis. | fusarium oxysporum f. sp. pisi (fop) is an important and destructive pathogen affecting pea crop (pisum sativum) throughout the world. control of this disease is achieved mainly by integration of different disease management procedures. however, the constant evolution of the pathogen drives the necessity to broaden the molecular basis of resistance to fop. our proteomic study was performed on pea with the aim of identifying proteins involved in different resistance mechanisms operating during f. ... | 2015 | 25672548 |
| rapid and efficient estimation of pea resistance to the soil-borne pathogen fusarium oxysporum by infrared imaging. | fusarium wilts are widespread diseases affecting most agricultural crops. in absence of efficient alternatives, sowing resistant cultivars is the preferred approach to control this disease. however, actual resistance sources are often overcome by new pathogenic races, forcing breeders to continuously search for novel resistance sources. selection of resistant accessions, mainly based on the evaluation of symptoms at timely intervals, is highly time-consuming. thus, we tested the potential of an ... | 2015 | 25671514 |
| [cloning and functional characterization of pathogenesis-related pr10-1 gene in panax notoginseng]. | with homology cloning approaches coupling with race (rapid-amplification of cdna ends) techniques, the full-length coding sequence of pathogenesis-related protein pr10-1 with differential expression was cloned from the total rna of the root of panax notoginseng, and its function was explored furtherly. as a result, the longest 465 bp orf (named as pnpr10-1 with the accession no. kj741402 in genbank) was detected from the cloned sequence with full-length of cdna of 863 bp. the corresponding pepti ... | 2015 | 25975034 |
| effects of water regime, crop residues, and application rates on control of fusarium oxysporum f. sp. cubense. | biological soil disinfestation is an effective method to control soil-borne disease by flooding and incorporating with organic amendments, but field conditions and resources sometimes limited its practical application. a laboratory experiment was conducted to develop practice guidelines on controlling fusarium wilt, a widespread banana disease caused by fusarium oxysporum f. sp. cubense (foc). foc infested soil incorporated with rice or maize straw at rates of 1.5 tons/ha and 3.0 tons/ha was inc ... | 2015 | 25968255 |
| the avr2-six5 gene pair is required to activate i-2-mediated immunity in tomato. | plant-invading microbes betray their presence to a plant by exposure of antigenic molecules such as small, secreted proteins called 'effectors'. in fusarium oxysporum f. sp. lycopersici (fol) we identified a pair of effector gene candidates, avr2-six5, whose expression is controlled by a shared promoter. the pathogenicity of avr2 and six5 fol knockouts was assessed on susceptible and resistant tomato (solanum lycopersicum) plants carrying i-2. the i-2 nb-lrr protein confers resistance to fol rac ... | 2015 | 25967461 |
| isolation of a hemagglutinin with potent antiproliferative activity and a large antifungal defensin from phaseolus vulgaris cv. hokkaido large pinto beans. | lectins (hemagglutinins) are defined as sugar-binding proteins or glycoproteins with various biological activities. a 60 kda dimeric hemagglutinin with a blocked n-terminus was isolated in large yield (190 mg/60 g) from the common edible bean phaseolus vulgaris cv. hokkaido large pinto bean. its hemagglutinating, antifungal, and antitumor activities as well as the effects of carbohydrate and metal ions on its hemagglutinating activity were examined. it inhibited the proliferation of nasopharynge ... | 2015 | 25965006 |
| demethylation of arsenic limits its volatilization in fungi. | arsenic (as) biomethylation is increasingly being regarded as a promising method to volatize as from the environment; however, the as volatilization efficiency of most microorganisms is low. here, the speciation transformation of dimethylarsinic acid (dma) as an important methylation intermediate in the cells of fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1 were investigated. these fungal strains have been certified to volatilize as from as-loade ... | 2015 | 25951513 |
| effect of eugenol against streptococcus agalactiae and synergistic interaction with biologically produced silver nanoparticles. | streptococcus agalactiae (group b streptococci (gbs)) is an important infections agent in newborns associated with maternal vaginal colonization. intrapartum antibiotic prophylaxis in gbs-colonized pregnant women has led to a significant reduction in the incidence of early neonatal infection in various geographic regions. however, this strategy may lead to resistance selecting among gbs, indicating the need for new alternatives to prevent bacterial transmission and even to treat gbs infections. ... | 2015 | 25945115 |
| disseminated fusariosis and hematologic malignancies, a still devastating association. report of three new cases. | fungi of the genus fusarium are primarily plant pathogens and saprobes that produce disseminated infections in immunologically deficient humans. after aspergillosis, disseminated fusariosis is the second most common cause of invasive infection by filamentous fungi in patients with hematologic malignancies or those undergoing transplants of hematopoietic progenitors. | 2015 | 25936697 |
| metabolic profiling of chickpea-fusarium interaction identifies differential modulation of disease resistance pathways. | chickpea is the third most widely grown legume in the world and mainly used as a vegetarian source of human dietary protein. fusarium wilt, caused by fusarium oxysporum f. sp. ciceri (foc), is one of the major threats to global chickpea production. host resistance is the best way to protect crops from diseases; however, in spite of using various approaches, the mechanism of foc resistance in chickpea remains largely obscure. in the present study, non-targeted metabolic profiling at several time ... | 2015 | 25935544 |
| characterization of novel trichoderma asperellum isolates to select effective biocontrol agents against tomato fusarium wilt. | the use of novel isolates of trichoderma with efficient antagonistic capacity against fusarium oxysporum f. sp. lycopersici (fol) is a promising alternative strategy to pesticides for tomato wilt management. we evaluated the antagonistic activity of 30 isolates of t. asperellum against 4 different isolates of fol. the production of extracellular cell wall degrading enzymes of the antagonistic isolates was also measured. the random amplified polymorphic dna (rapd) method was applied to assess the ... | 2015 | 25774110 |
| isolation, purification, and characterization of a stable defensin-like antifungal peptide from trigonella foenum-graecum (fenugreek) seeds. | a novel defensin-like antifungal peptide (tf-afp) with molecular mass of 10.3 kda was isolated from seeds of trigonella foenum-graecum (fenugreek) by ammonium sulfate precipitation, cation-exchange, gel-filtration, hydrophobic chromatography, and rp-hplc. mass spectroscopic analysis revealed the intact mass of the purified antifungal peptide as 10321.5 da and high similarity to plant defensins and other antifungal proteins in database search. 2d-page showed pi value to be 8.8 and absence of isof ... | 2015 | 25761687 |
| interaction of pseudostellaria heterophylla with fusarium oxysporum f.sp. heterophylla mediated by its root exudates in a consecutive monoculture system. | in this study, quantitative real-time pcr (qpcr) was used to determine the amount of fusarium oxysporum, an important replant disease pathogen in pseudostellaria heterophylla rhizospheric soil. moreover, hplc was used to identify phenolic acids in root exudates then it was further to explore the effects of the phenolic acid allelochemicals on the growth of f. oxysporum f.sp. heterophylla. the amount of f. oxysporum increased significantly in p. heterophylla rhizosphere soil under a consecutive r ... | 2015 | 25645742 |
| [antibacterial components from artificially induced dragon's blood of dracaena cambodiana]. | ten compounds were isolated from the artificially induced dragon's blood of dracaena cambodiana by various column chromatographies on silica and sephadex lh-20 gel. based on spectral analysis of nmr and ms, their structures were identified as 3, 4-dihydroxyallylbenzene (1), 3', 4', 5'-trimethoxycinnamylalcohol (2), pinoresinol (3), (2r)-7, 4'-dihydroxy-8-methylflavane (4), (2r)-7,4'-dihydroxy-5-methoxy-8-methylflavane(5),(2s)-7,3'-dihydroxy-4'-methoxy-8-methylflavane(6) ,(2s)-4',7-dihydroxy-6, 8 ... | 2015 | 27062817 |
| identification of a novel small cysteine-rich protein in the fraction from the biocontrol fusarium oxysporum strain cs-20 that mitigates fusarium wilt symptoms and triggers defense responses in tomato. | the biocontrol effect of the non-pathogenic fusarium oxysporum strain cs-20 against the tomato wilt pathogen f. oxysporum f. sp. lycopersici (fol) has been previously reported to be primarily plant-mediated. this study shows that cs-20 produces proteins, which elicit defense responses in tomato plants. three protein-containing fractions were isolated from cs-20 biomass using size exclusion chromatography. exposure of seedling roots to one of these fractions prior to inoculation with pathogenic f ... | 2015 | 26779237 |