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antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa.plants harbor endophytes with potential bioactivity. markhamia tomentosa (benth) k. schum ex. engl. (bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities.201727937112
field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens.effective disease management strategies to prevent catastrophic crop losses require rapid, sensitive, and multiplexed detection methods for timely decision making. to address this need, a rapid, highly specific and sensitive point-of-care method for multiplex detection of plant pathogens was developed by taking advantage of surface-enhanced raman scattering (sers) labeled nanotags and recombinase polymerase amplification (rpa), which is a rapid isothermal amplification method with high specifici ...201627403651
parasitic weed management by using strigolactone-degrading fungi.seed germination is a key phase of the parasitic plant life cycle that is stimulated by the secondary metabolites, mainly strigolactones (sls), secreted by the host roots. interventions during this stage would be particularly suitable for parasitic weed management practices, as blocking these chemical signals would prevent seed germination and thus parasite attack. four fungal strains with different ecological functions were considered for their possible ability to metabolise sls: fusarium oxysp ...201626757233
cremenolide, a new antifungal, 10-member lactone from trichoderma cremeum with plant growth promotion activity.trichoderma based products are considered an alternative to synthetic pesticides and fertilizers. these trichoderma spp. are among the most studied and applied fungal bcas in industry and agriculture and are known to secrete several secondary metabolites with different biological activities. the analysis of metabolic profiles (the 'metabolome') of trichoderma species is complex because of the wide range of compounds produced and the molecular activities identified, including the recently determi ...201626728227
characterization, expression patterns and functional analysis of the mapk and mapkk genes in watermelon (citrullus lanatus).mitogen-activated protein kinase (mapk) cascades, which consist of three functionally associated protein kinases, namely mekks, mkks and mpks, are universal signaling modules in all eukaryotes and have been shown to play critical roles in many physiological and biochemical processes in plants. however, little or nothing is known about the mpk and mkk families in watermelon.201526700161
lunatin, a novel lectin with antifungal and antiproliferative bioactivities from phaseolus lunatus billb.a novel lectin with a molecular mass of 24.3kda, designated lunatin, was isolated from edible seeds of phaseolus lunatus billb. the purification scheme consisted of ammonium sulfate precipitation, affinity chromatography, ion exchange chromatography, and gel filtration chromatography. the lectin is a glycoprotein, as determined by staining with periodic acid-schiff (pas), and its n-terminal amino acid sequence was determined to be daviyrgpgdlhtgs. lunatin exhibited hemagglutinating activity towa ...201627164500
isolation and antifungal activity of methyl 2,3-dihydroxybenzoate from paenibacillus elgii hoa73.the aim of the present study is to describe the purification and identification of methyl 2,3-dihydroxybenzoate (m2,3db), isolated for the first time from paenibacillus elgii hoa73, and to subsequently investigate its antifungal activity against important plant pathogens. the results show that m2,3db can be purified by many different chromatographic techniques and is identified as methyl 2,3-dihydroxybenzoate based on nuclear magnetic resonance (nmr) and liquid chromatography-mass spectrometry ( ...201626796297
experimental protocol for the recovery and evaluation of bioactive compounds of tarbush against postharvest fruit fungi.the aim of this study was to recover and evaluate in vitro the antifungal activity of bioactive compounds of tarbush flourensia cernua against fruit postharvest fungi and their antioxidant capacity. a yield of 15% of bioactive compounds of tarbush was obtained by infusion method and heating using water as solvent. a concentration of 4000 mg/l showed a higher antioxidant activity against the abts radical (3.21 μmol/g) in comparison with the dpph radical (7.62 μmol/g); however the dpph radical sho ...201626769505
the shewanella algae strain ym8 produces volatiles with strong inhibition activity against aspergillus pathogens and aflatoxins.aflatoxigenic aspergillus fungi and associated aflatoxins are ubiquitous in the production and storage of food/feed commodities. controlling these microbes is a challenge. in this study, the shewanella algae strain ym8 was found to produce volatiles that have strong antifungal activity against aspergillus pathogens. gas chromatography-mass spectrometry profiling revealed 15 volatile organic compounds (vocs) emitted from ym8, of which dimethyl trisulfide was the most abundant. we obtained authent ...201526500631
purification and characterization of a lectin from green split peas (pisum sativum).lectins have captured the attention of a large number of researchers on account of their various exploitable activities, including antitumor, immunomodulatory, antifungal, as well as hiv reverse transcriptase inhibitory activities. a mannose/glucose-specific lectin was isolated from green split peas (a variety of pisum sativum) and characterized. the purification step involved anion-exchange chromatography on a deae-cellulose column, cation-exchange chromatography on an sp-sepharose column, and ...201526304129
synergistic action of a metalloprotease and a serine protease from fusarium oxysporum f. sp. lycopersici cleaves chitin-binding tomato chitinases, reduces their antifungal activity, and enhances fungal virulence.as part of their defense strategy against fungal pathogens, plants secrete chitinases that degrade chitin, the major structural component of fungal cell walls. some fungi are not sensitive to plant chitinases because they secrete chitin-binding effector proteins that protect their cell wall against these enzymes. however, it is not known how fungal pathogens that lack chitin-binding effectors overcome this plant defense barrier. here, we investigated the ability of fungal tomato pathogens to cle ...201525915453
naturally produced citral can significantly inhibit normal physiology and induce cytotoxicity on magnaporthe grisea.given the importance of finding alternatives to synthetic fungicides, the antifungal effects of natural product citral on six plant pathogenic fungi (magnaporthe grisea, gibberella zeae, fusarium oxysporum, valsa mali, botrytis cinerea, and rhizoctonia solani) were determined. mycelial growth rate results showed that citral possessed high antifungal activities on those test fungi with ec50 values ranging from 39.52 to 193.00 µg/ml, which had the highest inhibition rates against m. grisea. furthe ...201525752425
phylogenic analysis revealed an expanded c₂h₂-homeobox subfamily and expression profiles of c₂h₂ zinc finger gene family in verticillium dahliae.c2h2 zinc finger (czf) proteins are a major class of transcription factors that play crucial roles in fungal growth, development, various stress responses, and virulence. little genome-wide data is available regarding the roles of czf proteins in verticillium dahliae, a destructive pathogen that causes vascular wilt disease in more than 200 plant species. we identified a total of 79 typical czf genes in v. dahliae. comparative analysis revealed that four plant pathogenic fungi, v. dahliae, fusar ...201525725127
solubility, photostability and antifungal activity of phenylpropanoids encapsulated in cyclodextrins.effects of the encapsulation in cyclodextrins (cds) on the solubility, photostability and antifungal activities of some phenylpropanoids (pps) were investigated. solubility experiments were carried out to evaluate the effect of cds on pps aqueous solubility. loading capacities and encapsulation efficiencies of freeze-dried inclusion complexes were determined. moreover, photostability assays for both inclusion complexes in solution and solid state were performed. finally, two of the most widespre ...201626593522
the importance of chorismate mutase in the biocontrol potential of trichoderma parareesei.species of trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. in addition to these abilities, trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. here we study the role in biocontrol of the t. parareesei tparo7 gene, encoding a chorismate mutase (cm), a s ...201526579090
the abc transporter abcg29 is involved in h2o2 tolerance and biocontrol traits in the fungus clonostachys rosea.for successful biocontrol interactions, biological control organisms must tolerate toxic metabolites produced by themselves or plant pathogens during mycoparasitic/antagonistic interactions, by host plant during colonization of the plant, and xenobiotics present in the environment. atp-binding cassette (abc) transporters can play a significant role in tolerance of toxic compounds by mediating active transport across the cellular membrane. this paper reports on functional characterization of an a ...201626520102
in vitro screening of antifungal activity of marine sponge extracts against five phytopathogenic fungi.the aim of our research is the screening of extracts of marine sponges for their antifungal activity against phytopathogenic fungi. the in vitro screening of hydroalcoholic and organic extracts of ten marine sponges from atlantic coast of morocco against five phytopathogenic fungi (fusarium oxysporum f.sp. melonis, fusarium oxysporum f.sp. radicis-lycopersici, fusarium oxysporum f.sp. ciceris, botrytis cinerea and penicillium digitatum) showed that only two sponges (haliclona viscosa and cynachi ...201425392799
design, synthesis and fungicidal activities of some novel pyrazole derivatives.in order to discover new compounds with good fungicidal activities, 32 pyrazole derivatives were designed and synthesized. the structures of the target compounds were confirmed by 1h-nmr, 13c-nmr, and high-resolution electrospray ionization mass spectrometry (hr-esi-ms), and their fungicidal activities against botrytis cinerea, rhizoctonia solani kuhn, valsa mali miyabe et yamada, thanatephorus cucumeris (frank) donk, fusarium oxysporum (s-chl) f.sp. cucumerinum owen, and fusarium graminearum sc ...201425203055
isolation and characteristics of protocatechuic acid from paenibacillus elgii hoa73 against botrytis cinerea on strawberry fruits.this study was undertaken to describe purification, identification, and characteristics of protocatechuic acid (pca) isolated for the first time from paenibacillus elgii hoa73 against botrytis cinerea (the cause of gray mold disease on strawberry fruit). pca was purified by different chromatographic techniques and identified as pca (3,4-dihydroxybenzoic acid) by nuclear magnetic resonance and liquid chromatography-mass spectrometry analyses. pca displayed potent antifungal activity against b. ci ...201525081931
molecular inversion probe: a new tool for highly specific detection of plant pathogens.highly specific detection methods, capable of reliably identifying plant pathogens are crucial in plant disease management strategies to reduce losses in agriculture by preventing the spread of diseases. we describe a novel molecular inversion probe (mip) assay that can be potentially developed into a robust multiplex platform to detect and identify plant pathogens. a mip has been designed for the plant pathogenic fungus fusarium oxysporum f.sp. conglutinans and the proof of concept for the effi ...201425343255
antifungal activity of paenibacillus kribbensis strain t-9 isolated from soils against several plant pathogenic fungi.the bacterial strain t-9, which shows strong antifungal activity, is isolated from the soils of samcheok, gangwondo and identified as paenibacillus kribbensis according to morphological and taxonomic characteristics and 16s rrna gene sequence analysis. the p. kribbensis strain t-9 strongly inhibits the growth of various phytopathogenic fungi including botrytis cinerea, colletotricum acutatum, fusarium oxysporum f. sp. radicis-lycopersici, magnaporthe oryzae, phytophthora capsici, rhizoctonia sol ...201425288992
pcr-based specific techniques used for detecting the most important pathogens on strawberry: a systematic review.strawberry diseases are a major limiting factor that severely impact plant agronomic performance. regarding limitations of traditional techniques for detection of pathogens, researchers have developed specific dna-based tests as sensitive and specific techniques. the aim of this review is to provide an overview of polymerase chain reaction (pcr)-based methods used for detection or quantification of the most widespread strawberry pathogens, such as fusarium oxysporum f.sp. fragariae, phytophthora ...201525588564
tandem combination of trigonella foenum-graecum defensin (tfgd2) and raphanus sativus antifungal protein (rsafp2) generates a more potent antifungal protein.plant defensins are small (45 to 54 amino acids) positively charged antimicrobial peptides produced by the plant species, which can inhibit the growth of a broad range of fungi at micro-molar concentrations. these basic peptides share a common characteristic three-dimensional folding pattern with one α-helix and three β-sheets that are stabilized by eight disulfide-linked cysteine residues. instead of using two single-gene constructs, it is beneficial when two effective genes are made into a sin ...201324022215
isolation of a trypsin-chymotrypsin inhibitor and its functional properties.a novel trypsin inhibitor with thermal and ph stability, designated merrtine, was isolated from glycine max l. merr. the procedure involved ammonium sulfate precipitation, ion-exchange chromatography on cm-sephadex c-50, and affinity chromatography on affi-gel blue gel. the 20 n-terminal amino acid sequences were determined to be deyskpccdlcmctrrcppq, demonstrating high homology with the sequence of bowman-birk type trypsin inhibitors. the molecular mass and isoelectric point of the inhibitor we ...201424499360
the elicitation effect of pathogenic fungi on trichodermin production by trichoderma brevicompactum.the effects of six species of phytopathogenic fungi mycelia as elicitors on trichodermin yield by trichoderma brevicompactum were investigated. neither nonviable nor viable mycelia of botrytis cinerea, alternaria solani, colletotrichum lindemuthianum, and thanatephorus cucumeris demonstrated any elicitation on the accumulation of trichodermin. however, the production of trichodermin was increased by the presence of viable/nonviable rhizoctonia solani and fusarium oxysporum mycelia. the strongest ...201324385883
induced resistance to botrytis cinerea in capsicum annuum by a fusarium crude elicitor fraction, free of proteins.fusarium oxysporum f. sp. lycopersici (fol) induces resistance in pepper against the airborne pathogen botrytis cinerea and the soil-borne pathogen verticillium dahliae. however, its practical use is limited due to its pathogenicity to other crops. in this study we tested several fractions of a heat-sterilised crude fol-elicitor preparation to protect pepper against b. cinerea and v. dahliae. only the protein-free insoluble fraction of the preparation reduced b. cinerea infection. however, none ...201324112636
the top 10 fungal pathogens in molecular plant pathology.the aim of this review was to survey all fungal pathologists with an association with the journal molecular plant pathology and ask them to nominate which fungal pathogens they would place in a 'top 10' based on scientific/economic importance. the survey generated 495 votes from the international community, and resulted in the generation of a top 10 fungal plant pathogen list for molecular plant pathology. the top 10 list includes, in rank order, (1) magnaporthe oryzae; (2) botrytis cinerea; (3) ...201222471698
prp8 inteins in species of the genus botrytis and other ascomycetes.the mobile elements termed inteins have a sporadic distribution in microorganisms. it is unclear how these elements are maintained. inteins are intervening protein sequences that autocatalytically excise themselves from a precursor. excision is a post-translational process referred to as 'protein splicing' in which the sequences flanking the intein are ligated, reforming the mature host protein. some inteins contain a homing endonuclease domain (heg) that is proposed to facilitate propagation of ...201222285471
expression of the ginseng pgpr10-1 in arabidopsis confers resistance against fungal and bacterial infection.korean ginseng (panax ginseng c. a. meyer) consists of nine cultivars from three jakyung, chungkyung, and hwangsook lines. among three previously identified pr-10 homologs from ginseng (pgpr10-1, pgpr10-2, and pgpr10-3), we found that the exact same sequence of pgpr10-2 exist in all tested nine cultivars. but a deletion and snp was found in american ginseng (panax quinquefolius). pr-10 proteins are known to be small and cytosolic, and showed similar three-dimensional structure. here we show that ...201222750300
purification, characterization of a ckchn134 protein from cynanchum komarovii seeds and synergistic effect with cktlp against verticillium dahliae.cynanchum komarovii al iljinski is a desert plant that has been used as analgesic, anthelminthic, and antidiarrheal, but also as herbal medicine to treat cholecystitis in people. in this work, an antifungal protein with sequence homology to chitinase was isolated from c. komarovii seeds and named ckchn134. the three-dimensional structure prediction of ckchn134 indicated that the protein has a loop domain formed a thin cleft, which is able to bind molecules and substrates. the protein and cktlp s ...201222532259
isolation and identification of the antimicrobial agent beauvericin from the endophytic fusarium oxysporum 5-19 with nmr and esi-ms/ms.endophytic microbe has been proved to be one of rich sources of bioactive natural products with potential application for new drug and pesticide discovery. one cyclodepsipeptide, beauvericin, was firstly isolated from the fermentation broth of fusarium oxysporum 5-19 endophytic on edgeworthia chrysantha linn. its chemical structure was unambiguously identified by a combination of spectroscopic methods, such as hresi-ms and (1)h and (13)c nmr. esi-ms/ms was successfully used to elucidate the spli ...201627413733
expression of recombinant earli1, a hybrid proline-rich protein of arabidopsis, in escherichia coli and its inhibition effect to the growth of fungal pathogens and saccharomyces cerevisiae.earli1 is an arabidopsis gene with pleiotropic effects previously shown to have auxiliary functions in protecting plants against freezing-induced cellular damage and promoting germinability under low-temperature and salinity stresses. here we determined whether recombinant earli1 protein has anti-fungal activity. recombinant earli1 protein lacking its signal peptide was produced in escherichia coli bl21(de3) using isopropyl β-d-1-thiogalactopyranoside (iptg) induction and the prokaryotic express ...201222759515
antifungal properties of hypericin, hypericin tetrasulphonic acid and fagopyrin on pathogenic fungi and spoilage yeasts.the role of hypericin-mediated photodynamic antimicrobial properties on pathogenic fungi and photodynamic therapy for human cancer cells is known. antifungal properties of hypericum perforatum l. (hypericaceae) and fagopyrum esculentum moench. (polygonaceae) extracts were also studied. the different polarities of solvents can cause complication in the identification of antifungal effects of separate biologically active compounds. in recent experimental work, we compared antifungal properties of ...201627564138
draft genome sequence of broad-spectrum antifungal bacterium burkholderia gladioli strain ngj1, isolated from healthy rice seeds.we report here the draft genome sequence of burkholderia gladioli strain ngj1. the strain was isolated from healthy rice seeds and exhibits broad-spectrum antifungal activity against several agriculturally important pathogens, including rhizoctonia solani, magnaporthe oryzae, venturia inaequalis, and fusarium oxysporum.201526205861
extraction, identification and antimicrobial activity of a new furanone, grifolaone a, from grifola frondosa.a furanone (1), (s)-methyl 2-(2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl)acetate, was isolated from the edible mushroom grifola frondosa. mass spectrometry and nmr analyses were used to elucidate the structure of this compound, and its absolute configuration was determined using circular dichroism spectroscopy. compound 1 exhibited specific antifungal activity against the plant pathogens, fusarium oxysporum, gibberella zeae and piricularia oryzae and the opportunistic human pathogen, pse ...201626374926
a new lupane-type triterpenoid fatty acid ester and other isolates from ophiorrhiza shendurunii.a new pentacyclic triterpenoid fatty acid ester, lupan-20-ol-3(β)-yl hexadecanoate (1), together with lupan-20-ol-3(β)-yl acetate (2), olean-18-en-3(β)-yl hexadecanoate (3), dotriacontanoic acid (4), stigmasterol (5), rubiadin (6), nonadecanoic acid (7), palmitic acid (8) and camptothecin (9) were isolated from the hexane and chloroform extracts of ophiorrhiza shendurunii from south india. structures of the isolates were determined by (1)h, (13)c, (13)c dept, (1)h-(1)h cosy, hmbc, hsqc, noesy nm ...201626979490
different forms of apolipophorin iii in galleria mellonella larvae challenged with bacteria and fungi.apolipophorin iii (apolp-iii), a lipid-binding protein and an insect homolog of human apolipoprotein e, plays an important role in lipid transport and immune response in insects. in the present study, we have demonstrated a correlation in time between changes in the apolp-iii abundance occurring in the hemolymph, hemocytes, and fat body after immunization of galleria mellonella larvae with gram-negative bacteria escherichia coli, gram-positive bacteria micrococcus luteus, yeast candida albicans, ...201525579437
a new model of in vitro fungal biofilms formed on human nail fragments allows reliable testing of laser and light therapies against onychomycosis.onychomycoses represent approximately 50 % of all nail diseases worldwide. in warmer and more humid countries like brazil, the incidence of onychomycoses caused by non-dermatophyte molds (ndm, including fusarium spp.) or yeasts (including candida albicans) has been increasing. traditional antifungal treatments used for the dermatophyte-borne disease are less effective against onychomycoses caused by ndm. although some laser and light treatments have demonstrated clinical efficacy against onychom ...201525471266
combination of fluconazole with silver nanoparticles produced by fusarium oxysporum improves antifungal effect against planktonic cells and biofilm of drug-resistant candida albicans.silver nanoparticles (agnps) have been extensively studied because of their anti-microbial potential. here, we evaluated the effect of biologically synthesized silver nanoparticles (agnpbio) alone and in combination with fluconazole (flc) against planktonic cells and biofilms of flc-resistant candida albicans agnpbio exhibited a fungicidal effect, with a minimal inhibitory concentration (mic) and fungicidal concentration ranging from 2.17 to 4.35 μg/ml. the combination of agnpbio and flc reduced ...201626092103
antimicrobial activity of engineered shrimp ovarian peritrophin fragments from fenneropenaeus merguiensis.shrimp ovarian peritrophin (sop), a major protein in jelly layer and cortical rods, plays a role in egg protection after spawning. previous study, sequence of sop gene from fenneropenaeus merguiensis (fm-sop) was composed of domain a and domain b. the sop domain a contains amino acid sequences between 1-80 of fm-sop. the domain a had six conserved cysteines which have been found in many antimicrobial peptides. the molecular weight of purified rsop-a protein was about 9 kda. the sop domain b cont ...201525159507
miltefosine is effective against candida albicans and fusarium oxysporum nail biofilms in vitro.onychomycosis is a fungal nail infection that represents ∼50 % of all nail disease cases worldwide. clinical treatment with standard antifungals frequently requires long-term systemic therapy to avoid chronic disease. onychomycosis caused by non-dermatophyte moulds, such as fusarium spp., and yeasts, such as candida spp., is particularly difficult to treat, possibly due to the formation of drug-resistant fungal biofilms on affected areas. here, we show that the alkylphospholipid miltefosine, use ...201526404553
comparative functional characterization of a novel benzoate hydroxylase cytochrome p450 of fusarium oxysporum.focyp53a19, a novel cytochrome p450 capable of performing benzoate hydroxylation, was identified and characterized from the ascomycete fusarium oxysporum f.sp. lycopersici. comparative functional analysis of focyp53a19 with the heterologous and homologous cytochrome p450 reductases (cpr) such as saccharomyces cerevisiae (sccpr), candida albicans (cacpr) and f. oxysporum (focpr) revealed novel catalytic properties. the catalytic efficiency and substrate specificity of focyp53a19 were significantl ...201525659633
the wor1-like protein fgp1 regulates pathogenicity, toxin synthesis and reproduction in the phytopathogenic fungus fusarium graminearum.wor1 is a gene for a conserved fungal regulatory protein controlling the dimorphic switch and pathogenicity determents in candida albicans and its ortholog in the plant pathogen fusarium oxysporum, called sge1, is required for pathogenicity and expression of key plant effector proteins. f. graminearum, an important pathogen of cereals, is not known to employ switching and no effector proteins from f. graminearum have been found to date that are required for infection. in this study, the potentia ...201222693448
disseminated phaeohyphomycosis in a dog.phaeohyphomycosis is a rare but emerging disease caused by dematiaceous fungi. here we describe the case of an immunosuppressed dog with disseminated phaeohyphomycosis secondary to bipolaris spicifera infection. regionally extensive infiltration of the paw pads, skin, myocardium, liver, renal interstitium and diaphragm was identified on histopathology. candida glabrata and fusarium oxysporum were also cultured from multiple sites post-mortem. the dog was treated with fluconazole, itraconazole, t ...201728275534
[biological activity of lipids and photosynthetic pigments of sargassum pallidum c. agardh].the biological activity of lipids and photosynthetic pigments of the kelp sargassum pallidum (turner) c. agardh has been studied. free fatty acids and their esters demonstrated considerable antimicrobial activity against bacteria (staphylococcus aureus[ital] and escherichia coli), yeast-like fungi (candida albicans), and opportunistic pathogenic (aspergilius niger) and phytopathogenic (fusarium oxysporum, and septoria glycines) fungi. glyceroglycolipids and neutral lipids demonstrated moderate a ...201425272757
insights into fungal communities in composts revealed by 454-pyrosequencing: implications for human health and safety.fungal community composition in composts of lignocellulosic wastes was assessed via 454-pyrosequencing of its1 libraries derived from the three major composting phases. ascomycota represented most (93%) of the 27,987 fungal sequences. a total of 102 genera, 120 species, and 222 operational taxonomic units (otus; >97% similarity) were identified. thirty genera predominated (ca. 94% of the sequences), and at the species level, sequences matching chaetomium funicola and fusarium oxysporum were the ...201323785368
prospecting fungal parasites of the potato cyst nematode globodera pallida using a rapid screening technique.seven filamentous fungal species were isolated from individual eggs of globodera pallida cysts collected from infested fields in shelley idaho, usa and identified as chaetomium globosum, fusarium oxysporum, fusarium solani, fusarium tricinctum, microdochium bolleyi, purpureocillium lilacinum, and plectosphaerella cucumerina. their ability to reduce infection by g. pallida in planta were assessed in simple, reproducible micro-rhizosphere chambers (micro-rocs). all fungi reduced g. pallida infecti ...201728375550
bioconjugation of gold and silver nanoparticles synthesized by fusarium oxysporum and their use in rapid identification of candida species by using bioconjugate-nano-polymerase chain reaction.we developed a bioconjugate-nano-pcr as a rapid and specific method for identification of candida species in less time. this requires very low concentration of master mix and dna sample of candida albicans in conjugation with gold nanoparticles (aunps) and silver nanoparticles (agnps). we report a modification of the pcr assay with nanoparticles that allows the detection of high fidelity amplification of its-rdna and beta (beta) tubulin gene of candida species from low concentrated dna in short ...201324266252
characterization of an endophytic whorl-forming streptomyces from catharanthus roseus stems producing polyene macrolide antibiotic.an endophytic whorl-forming streptomyces sp. designated as ts3ro having antifungal activity against a large number of fungal pathogens, including sclerotinia sclerotiorum, rhizoctonia solani, colletotrichum gloeosporioides, cryphonectria parasitica, fusarium oxysporum, pyrenophora tritici-repentis, epidermophyton floccosum, and trichophyton rubrum, was isolated from surface-sterilized catharanthus roseus stems. preliminary identification showed that streptomyces cinnamoneus subsp. sparsus was it ...201222524528
hybrid molecules of carvacrol and benzoyl urea/thiourea with potential applications in agriculture and medicine.benzoyl phenyl urea, a class of insect growth regulator's acts by inhibiting chitin synthesis. carvacrol, a naturally occurring monoterpenoid is an effective antifungal agent. we have structurally modified carvacrol (2-methyl-5-[1-methylethyl] phenol) by introducing benzoylphenyl urea linkage. two series of benzoylcarvacryl thiourea (bctu, 4a-f) and benzoylcarvacryl urea (bcu, 5a-f) derivatives were prepared and characterized by elemental analysis, ir, (1)h and (13)c nmr and mass spectroscopy. d ...201222850211
candida and fusarium species known as opportunistic human pathogens from customer-accessible parts of residential washing machines.energy constraints have altered consumer practice regarding the use of household washing machines. washing machines were developed that use lower washing temperatures, smaller amounts of water and biodegradable detergents. these conditions may favour the enrichment of opportunistic human pathogenic fungi. we focused on the isolation of fungi from two user-accessible parts of washing machines that often contain microbial biofilms: drawers for detergents and rubber door seals. out of 70 residentia ...201525749362
diversity of endophytic fungi in glycine max.endophytic fungi are microorganisms that live within plant tissues without causing disease during part of their life cycle. with the isolation and identification of these fungi, new species are being discovered, and ecological relationships with their hosts have also been studied. in glycine max, limited studies have investigated the isolation and distribution of endophytic fungi throughout leaves and roots. the distribution of these fungi in various plant organs differs in diversity and abundan ...201526111593
marine-derived penicillium in korea: diversity, enzyme activity, and antifungal properties.the diversity of marine-derived penicillium from korea was investigated using morphological and multigene phylogenetic approaches, analyzing sequences of the internal transcribed spacer region, β-tubulin gene, and rna polymerase subunit ii gene. in addition, the biological activity of all isolated strains was evaluated. we tested for the extracellular enzyme activity of alginase, endoglucanase, and β-glucosidase, and antifungal activity against two plant pathogens (colletotrichum acutatum and fu ...201424908060
identification of fungal phytopathogens using fourier transform infrared-attenuated total reflection spectroscopy and advanced statistical methods.the early diagnosis of phytopathogens is of a great importance; it could save large economical losses due to crops damaged by fungal diseases, and prevent unnecessary soil fumigation or the use of fungicides and bactericides and thus prevent considerable environmental pollution. in this study, 18 isolates of three different fungi genera were investigated; six isolates of colletotrichum coccodes, six isolates of verticillium dahliae and six isolates of fusarium oxysporum. our main goal was to dif ...201222352668
application and bioactive properties of cati, a trypsin inhibitor from capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells.during the last few years, a growing number of antimicrobial peptides have been isolated from plants and particularly from seeds. recent results from our laboratory have shown the purification of a new trypsin inhibitor, named cati, from chilli pepper (capsicum annuum l.) seeds. this study aims to evaluate the antifungal activity and mechanism of action of cati on phytopathogenic fungi and detect the presence of protease inhibitors in other species of this genus.201728139827
a novel and effective streptomyces sp. n2 against various phytopathogenic fungi.phytopathogenic fungi would induce a variety of plant diseases, resulting in a severe reduction of agricultural output. however, the current plant disease control is mainly dependent on the environmentally and healthily hazardous chemical fungicides. thus, the present work aimed to isolate an effective antagonistic microorganism against various soilborne phytopathogenic fungi. by dual culture with rhizoctonia solani, a novel streptomyces specie, streptomyces sp. n2, was screened out from a total ...201526306529
manganese lipoxygenase of f. oxysporum and the structural basis for biosynthesis of distinct 11-hydroperoxy stereoisomers.the biosynthesis of jasmonates in plants is initiated by 13s-lipoxygenase (lox), but details of jasmonate biosynthesis by fungi, including fusarium oxysporum, are unknown. the genome of f. oxysporum codes for linoleate 13s-lox (foxlox) and for f. oxysporum manganese lox (fo-mnlox), an uncharacterized homolog of 13r-mnlox of gaeumannomyces graminis. we expressed fo-mnlox and compared its properties to cg-mnlox from colletotrichum gloeosporioides. electron paramagnetic resonance and metal analysis ...201526113537
identification of volatile compounds produced by the bacterium burkholderia tropica that inhibit the growth of fungal pathogens.it has been documented that bacteria from the burkholderia genera produce different kinds of compounds that inhibit plant pathogens, however in burkholderia tropica, an endophytic diazotrophic and phosphate-solubilizing bacterium isolated from a wide diversity of plants, the capacity to produce antifungal compounds has not been evaluated. in order to expand our knowledge about burkholderia tropica as a potential biological control agent, we analyzed 15 different strains of this bacterium to eval ...201523680857
composition and antipathogenic activities of the twig essential oil of chamaecyparis formosensis from taiwan.in this study, antipathogenic activities of the twig essential oil and its constituents from chamaecyparis formosensis matsum were evaluated in vitro against six plant pathogenic fungi. the essential oil from the fresh twigs was isolated using hydrodistillation in a clevenger-type apparatus, and characterized by gc-fid and gc-ms. twenty-five compounds were identified, representing 98.9% of the oil. the main components were beta-eudesmol (25.1%), tau-muurolol (21.6%), elemol (15.0%), totarol (14. ...201222908586
antifungal activities of triterpenoids from the roots of astilbe myriantha diels.during a search for plant extracts that possess activity against the fungi that cause diseases in plants, a 95% ethanol extract from the roots of astilbe myriantha diels showed inhibitory activity against colletotrichum gloeosporioides. bioassay-guided fractionation of the 95% ethanol extract led to the isolation of seven triterpenoids (1-7). their structures were elucidated by spectroscopy and by a comparison with literature data. among these compounds, 3β,6β,24-trihydroxyurs-12-en-27-oic acid ...201125212161
elucidation of biocontrol mechanisms of trichoderma harzianum against different plant fungal pathogens: universal yet host specific response.in the present study, different transcripts of trichoderma harzianum thhp-3 were evaluated for their response against four fungal pathogens fusarium oxysporum, colletotrichum capsici, colletotrichum truncatum and gloesercospora sorghi using rt-qpcr. the time course study of t. harzianum transcripts related to signal transduction, lytic enzymes, secondary metabolites and various transporters revealed variation in expression against four fungal pathogens. in a broader term, the transcripts were up ...201727856319
biological characteristics and antimicrobial activity of endophytic streptomyces sp. tqr12-4 isolated from elite citrus nobilis cultivar ham yen of vietnam.ham yen orange (citrus nobilis lour) is the highly valuable commercial fruit of vietnam. with the blooming of fruit production and farming area, this specialty crop is facing threats from several serious diseases; therefore the search for new effective biocontrollers is required to prevent the existing excessive use of fertilizers and plant protection chemicals. endophytic actinomycetes are of great scientific interest due to their high potential of application in agriculture and pharmaceutical ...201627795709
investigating the compatibility of the biocontrol agent clonostachys rosea ik726 with prodigiosin-producing serratia rubidaea s55 and phenazine-producing pseudomonas chlororaphis toza7.this study was carried out to assess the compatibility of the biocontrol fungus clonostachys rosea ik726 with the phenazine-producing pseudomonas chlororaphis toza7 or with the prodigiosin-producing serratia rubidaea s55 against fusarium oxysporum f. sp. radicis-lycopersici. the pathogen was inhibited by both strains in vitro, whereas c. rosea displayed high tolerance to s. rubidaea but not to p. chlororaphis. we hypothesized that this could be attributed to the atp-binding cassette (abc) protei ...201626860841
endophytic fungi isolated from oil-seed crop jatropha curcas produces oil and exhibit antifungal activity.jatropha curcas l., a perennial plant grown in tropics and subtropics is popularly known for its potential as biofuel. the plant is reported to survive under varying environmental conditions having tolerance to stress and an ability to manage pest and diseases. the plant was explored for its endophytic fungi for use in crop protection. endophytic fungi were isolated from leaf of jatropha curcas, collected from new delhi, india. four isolates were identified as colletotrichum truncatum, and other ...201323409154
synthesis of 4'-thiosemicarbazonegriseofulvin and its effects on the control of enzymatic browning and postharvest disease of fruits.4'-thiosemicarbazonegriseofulvin, a new thiosemicarbazide derivative of griseofulvin, was synthesized and evaluated for its potential in the control of enzymatic browning and postharvest disease of fruits. browning on fruits is mainly due to the enzymatic oxidation of phenolic compounds catalyzed by tyrosinase. 4'-thiosemicarbazonegriseofulvin could effectively inhibit the activity of tyrosinase, and its 50% inhibitory concentration (ic(50)) against tyrosinase was determined to be 37.8 μm. it wa ...201223025498
suppressive effects of mycoviral proteins encoded by magnaporthe oryzae chrysovirus 1 strain a on conidial germination of the rice blast fungus.magnaporthe oryzae chrysovirus 1 strain a (mocv1-a) is the causal agent of growth repression and attenuated virulence (hypovirulence) of the rice blast fungus, magnaporthe oryzae. we previously revealed that heterologous expression of the mocv1-a orf4 protein resulted in cytological damage to the yeasts saccharomyces cerevisiae and cryptococcus neoformans. since the orf4 protein is one of the components of viral particles, we evaluated the inhibitory effects of the purified virus particle agains ...201627329666
synergistic effect of dazomet soil fumigation and clonostachys rosea against cucumber fusarium wilt.soil fumigation and biological control are two control measures frequently used against soilborne diseases. in this study, the chemical fumigant dazomet was applied in combination with the biocontrol agent (bca) clonostachys rosea 67-1 to combat cucumber wilt caused by fusarium oxysporum f. sp. cucumerinum kw2-1. when the mycoparasite c. rosea 67-1 was applied after dazomet fumigation, disease control reached 100%, compared with 88.1 and 69.8% for dazomet and 67-1 agent, respectively, applied al ...201424941326
low utility of pediatric isolator blood culture system for detection of fungemia in children: a 10-year review.the use of the wampole isolator 1.5-ml pediatric blood culture tube for the detection of fungemia in children was assessed by a 10-year retrospective review at two pediatric hospitals, the hospital for sick children in toronto, canada, and the children's medical center of dallas, texas. over this period, a total of 9,442 pediatric isolator specimens were processed, with yeast or yeast-like organisms recovered in 297 (3.1%) of the specimens (151 [1.6%] unique clinical episodes) and filamentous or ...201627307462
differential response of extracellular proteases of trichoderma harzianum against fungal phytopathogens.in the present study, production of extracellular proteases by trichoderma harzianum was evaluated based on the relative gene expression and spectrophotometric assay. the fungal isolates were grown in czapek dox broth medium supplemented with deactivated mycelium of plant fungal pathogens such as fusarium oxysporum, colletotrichum capsici, gloeocercospora sorghi, and colletotrichum truncatum. the maximum protease activity was detected after 48 h of incubation against colletotrichum spp. similarl ...201627278806
natural products as sources of new fungicides (ii): antiphytopathogenic activity of 2,4-dihydroxyphenyl ethanone derivatives.a series of 17 simple 1-(2,4-dihydroxyphenyl) ethanones were synthesised, and their structures characterised by (1)h, (13)c nmr and esi-ms. their in vitro antifungal activities were evaluated against five phytopathogenic fungi including glomerella cingulate, botrytis cirerea, fusarium graminearum, curvularia lunata and fusarium oxysporum f. sp. vasinfectum by the mycelial growth inhibition assay. compounds 2g and 2h exhibited broad-spectrum inhibitory activity against the mycelial growth of the ...201625978007
in vitro antifungal potentials of bioactive compound oleic acid, 3-(octadecyloxy) propyl ester isolated from lepidagathis cristata willd. (acanthaceae) inflorescence.to identify bioactive compound oleic acid, 3-(octadecyloxy) propyl ester from lepidagathis cristata willd. (l. cristata) and to assess antifungal potentials of the isolated compound.201425312119
synthesis and in vitro antifungal activities of new 2-aryl-6,7-methylenedioxy-3,4-dihydroisoquinolin-2-ium bromides.2-aryl-3,4-dihydroisoquinolin-2-iums might be considered as a class of simple analogues of natural quaternary benzo[c]phenanthridine alkaloids. in this paper, 26 new 2-aryl-6,7-methylenedioxy-3,4-dihydroisoquinolin-2-ium bromides with various substituents in n-aromatic ring were synthesized from commercially available 1,3-benzodioxole in good to excellent yields. all the compounds were elucidated by ms, high resolution (hr)-ms, ir, (1)h- and (13)c-nmr analysis, and evaluated for antifungal activ ...201323666271
delftia tsuruhatensis wgr-uom-bt1, a novel rhizobacterium with pgpr properties from rauwolfia serpentina (l.) benth. ex kurz also suppresses fungal phytopathogens by producing a new antibiotic-amtm.the bacterial strain designated as wgr-uom-bt1 isolated from rhizosphere of rauwolfia serpentina exhibited broad-spectrum antifungal activity and also improved early plant growth. based on morphological, biochemical and 16s rrna gene sequence analyses, the strain bt1 was identified as delftia tsuruhatensis (kf727978). under in vitro conditions, the strain bt1 suppressed the growth of wide range of fungal phytopathogens. purified antimicrobial metabolite from the strain bt1 was identified as nitr ...201526258398
clustering of two genes putatively involved in cyanate detoxification evolved recently and independently in multiple fungal lineages.fungi that have the enzymes cyanase and carbonic anhydrase show a limited capacity to detoxify cyanate, a fungicide employed by both plants and humans. here, we describe a novel two-gene cluster that comprises duplicated cyanase and carbonic anhydrase copies, which we name the cca gene cluster, trace its evolution across ascomycetes, and examine the evolutionary dynamics of its spread among lineages of the fusarium oxysporum species complex (hereafter referred to as the fosc), a cosmopolitan cla ...201525663439
silver nanoparticle production by the fungus fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts.the microbial synthesis of nanoparticles is a green chemistry approach that combines nanotechnology and microbial biotechnology. the aim of this study was to obtain silver nanoparticles (snps) using aqueous extract from the filamentous fungus fusarium oxysporum as an alternative to chemical procedures and to evaluate its antifungal activity. snps production increased in a concentration-dependent way up to 1 mm silver nitrate until 30 days of reaction. monodispersed and spherical snps were predom ...201424714966
recombinant exochitinase of the thermophilic mould myceliopthora thermophila bja: characteristics and utility in generating n-acetyl glucosamine and in biocontrol of phytopathogenic fungi.chitinase from the thermophilic mould myceliopthora thermophila bja (mtchit) is an acid tolerant, thermostable and organic solvent stable biocatalyst which does not require any metal ions for its activity. to produce high enzyme titres, reduce fermentation time and overcome the need for induction, this enzyme has been heterologously expressed under gap promoter in the gras yeast, pichia pastoris. the production medium supplemented with the permeabilizing agent tween-20 supported two-fold higher ...201727689686
green synthesis, characterisation and biological evaluation of agnps using agave americana, mentha spicata and mangifera indica aqueous leaves extract.the current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (agnps) of agave americana, mentha spicata and mangifera indica leaves and to screen them for biological activities. the ultraviolet-visible spectroscopic analysis revealed that λ-max for agnps range from 350-500 nm. all agnps possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in x-ray diffraction measurements an ...201627676375
comparative antibacterial activity of silver nanoparticles synthesised by biological and chemical routes with pluronic f68 as a stabilising agent.the authors report the comparative antibacterial activity of silver nanoparticles synthesised by biological (using fusarium oxysporum) and chemical routes in the presence and absence of pluronic f68 as a stabilising agent. the production of silver nanoparticles was evidenced by uv-visible spectra, with absorbance at about 420 nm in the case of both biological and chemical synthesis. x-ray diffraction pattern confirmed the presence of face-centred cubic structure (fcc plane). the nanoparticles ch ...201627463790
[identification of pathogens causing root rot disease on ligusticum chuanxiong in sichuan].to identify the pathogens causing root rot disease on ligusticum chuanxiong from the main producing areas in sichuan, and to provide gist of management of this disease in the future.201526495640
[overexpression, homology modeling and coenzyme docking studies of the cytochrome p450nor2 from cylindrocarpon tonkinense].cytochrome p450nor catalyzes an unusual reaction that transfers electrons from nadp/nadph to bound heme directly. to improve the expression level of p450nor2 from cylindrocarpon tonkinense (c.p450nor2), escherichia coli system was utilized to substitute the yeast system we constructed for expression of the p450nor2 gene, and the protein was purified in soluble form using ni(+)-nta affinity chromatography. in contrast to p450nor from fusarium oxysporum (f.p450nor) and p450nor1 from cylindrocarpon ...201627239859
seed vectored endophytic bacteria modulate development of rice seedlings.the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens.201728375579
novel phl-producing genotypes of finger millet rhizosphere associated pseudomonads and assessment of their functional and genetic diversity.genetic diversity of phld gene, an essential gene in the biosynthesis of 2,4-diacetylphloroglucinol, was studied by restriction fragment length polymorphism (rflp) in 20 phl-producing pseudomonads isolated from finger millet rhizosphere. rflp analysis of phld gene displayed three patterns with haeiii and taqi enzymes. phld gene sequence closely correlated with rflp results and revealed the existence of three new genotypes g, h and i. further, the phylogenetic and concatenated sequence analysis o ...201424819774
microbial host selection and periplasmic folding in escherichia coli affect the biochemical characteristics of a cutinase from fusarium oxysporum.a cutinase from the mesophilic fungus fusarium oxysporum (focut5a) was functionally expressed in different hosts and their recombinant products were characterized regarding their activity, thermostability and tolerance in organic solvents. the cutinase gene cut5a was expressed in the bl21 and origami 2 escherichia coli strains and the resulting protein was folded either in the cytoplasm or in the periplasmic space, with the aim of correct formation of disulfide bonds. increase of thermostability ...201627302766
structural and functional studies of a fusarium oxysporum cutinase with polyethylene terephthalate modification potential.cutinases are serine hydrolases that degrade cutin, a polyester of fatty acids that is the main component of plant cuticle. these biocatalysts have recently attracted increased biotechnological interest due to their potential to modify and degrade polyethylene terephthalate (pet), as well as other synthetic polymers.201526291558
comparative analysis of biosynthesised and chemosynthesised silver nanoparticles with special reference to their antibacterial activity against pathogens.the present study reports the synthesis of silver nanoparticles (agnps) using both biological and chemical routes to find out the best method for control of their size and activity. the fungal agent (fusarium oxysporum) and the plant (azadirachta indica) were found to be the best source for agnps synthesis. both biosynthesis and chemosynthesis were achieved by challenging filtrate with agno3 (1 mm) solution. the synthesised nanoparticles were characterised by ultraviolet-visible spectroscopy, fo ...201526023154
microbial contamination of domiciliary nebulisers and clinical implications in chronic obstructive pulmonary disease.domiciliary nebulisers are widely used in chronic obstructive pulmonary disease (copd) but nebuliser cleaning practice has not been assessed in patients with copd who are often elderly and may have severe disease and multiple comorbidities. we aimed to evaluate microbial contamination of home nebulisers used by patients with copd.201425478172
chitinase a from stenotrophomonas maltophilia shows transglycosylation and antifungal activities.stenotrophomonas maltophilia chitinase (stmchia and stmchib) genes were cloned and expressed as soluble proteins of 70.5 and 41.6 kda in escherichia coli. ni-nta affinity purified stmchia and stmchib were optimally active at ph 5.0 and 7.0, respectively and exhibited broad range ph activity. stmchia and stmchib had an optimum temperature of 40°c and are stable up to 50 and 40°c, respectively. hydrolytic activity on chitooligosaccharides indicated that stmchia was an endo-acting enzyme releasing ...201323428818
galactose oxidase from fusarium oxysporum--expression in e. coli and p. pastoris and biochemical characterization.a gene coding for galactose 6-oxidase from fusarium oxysporum g12 was cloned together with its native preprosequence and a c-terminal his-tag, and successfully expressed both in escherichia coli and pichia pastoris. the enzyme was subsequently purified and characterized. among all tested substrates, the highest catalytic efficiency (kcat/km) was found with 1-methyl-β-d-galactopyranoside (2.2 mm(-1) s(-1)). the michaelis constant (km) for d-galactose was determined to be 47 mm. optimal ph and tem ...201424967652
the rhizospheres of traditional medicinal plants in panxi, china, host a diverse selection of actinobacteria with antimicrobial properties.actinobacteria are a prolific source of antibiotics. since the rate of discovery of novel antibiotics is decreasing, actinobacteria from unique environments need to be explored. in particular, actinobacterial biocontrol strains from medicinal plants need to be studied as they can be a source of potent antibiotics. we combined culture-dependent and culture-independent methods in analyzing the actinobacterial diversity in the rhizosphere of seven traditional medicinal plant species from panxi, chi ...201222286515
characterization of a β-1,3-glucan recognition protein from the beet armyworm, spodoptera exigua (insecta: lepidoptera: noctuidae).the β-1,3-glucan recognition protein gene from spodoptera exigua (seβgrp) was cloned and characterized. the cdna of this gene is 1 644 nucleotides in length and the predicted polypeptide is 491 amino acids (aa) in length, with a calculated molecular mass of 54.8 kda. the first 22 aa encode a predicted secretion signal peptide. a blast search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of seβgrp revealed that this protein is most similar to the β-1,3-glucan recognit ...201323956146
an iron 13s-lipoxygenase with an α-linolenic acid specific hydroperoxidase activity from fusarium oxysporum.jasmonates constitute a family of lipid-derived signaling molecules that are abundant in higher plants. the biosynthetic pathway leading to plant jasmonates is initiated by 13-lipoxygenase-catalyzed oxygenation of α-linolenic acid into its 13-hydroperoxide derivative. a number of plant pathogenic fungi (e.g. fusarium oxysporum) are also capable of producing jasmonates, however, by a yet unknown biosynthetic pathway. in a search for lipoxygenase in f. oxysporum, a reverse genetic approach was use ...201323741422
a non-cyclic baboon θ-defensin derivative exhibiting antimicrobial activity against the phytopathogen verticillium dahliae.θ-defensins are the only natural cyclic proteins found in primates. they have strong antimicrobial activity related to their trisulfide ladders and macrocyclic conformation. a non-cyclic baboon θ-defensin (btd) was synthesized by substituting valine with phenylalanine at position 17, at the c-terminal end of the btd; this was termed "btd-s." the antimicrobial activities of this synthetic peptide were investigated against escherichia coli and two cotton phytopathogens: verticillium dahliae and fu ...201322903319
molecular cloning and functional analysis of a recombinant ribosome-inactivating protein (alpha-momorcharin) from momordica charantia.alpha-momorcharin (α-mc), a member of the ribosome-inactivating protein (rip) family, has been used not only as antiviral, antimicrobial, and antitumor agents, but also as toxicant to protozoa, insects, and fungi. in this study, we expressed the protein in escherichia coli rosetta (de3) plyss strain and purified it by nickel-nitrilotriacetic acid affinity chromatography. a total of 85 mg of homogeneous protein was obtained from 1 l culture supernatant of rosetta (de3) plyss, showing a high recov ...201222262229
heterotrophic bioleaching of sulfur, iron, and silicon impurities from coal by fusarium oxysporum fe and exophiala spinifera fm with growing and resting cells.coal is the most abundant fossil fuel containing sulfur and other elements which promote environmental pollution after burning. also the silicon impurities make the transportation of coal expensive. in this research, two isolated fungi from oil contaminated soil with accessory number kf554100 (fusarium oxysporum fe) and kc925672 (exophiala spinifera fm) were used for heterotrophic biological leaching of coal. the leaching were detected by ftir, chns, xrf analyzer and compared with iron and sulfa ...201626883128
wildly growing asparagus (asparagus officinalis l.) hosts pathogenic fusarium species and accumulates their mycotoxins.asparagus officinalis l. is an important crop in many european countries, likely infected by a number of fusarium species. most of them produce mycotoxins in plant tissues, thus affecting the physiology of the host plant. however, there is lack of information on fusarium communities in wild asparagus, where they would definitely have considerable environmental significance. therefore, the main scientific aim of this study was to identify the fusarium species and quantify their typical mycotoxins ...201626687343
fusarium species isolated from pennisetum clandestinum collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201425686222
<i>fusarium</i> species isolated from <i>pennisetum clandestinum</i> collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201428235286
root-associated fungi of rosa rugosa grown on the frontal dunes of the baltic sea coast in lithuania.the aim of the present study was to assess fungal communities associated with fine living roots of rosa rugosa thunb grown on the frontal dunes of curonian spit at the baltic sea coast in lithuania. the roots of r. rugosa were sampled at five sites (nida, preila, pervalka, juodkrante and smiltyne) situated at a distance ca. 5-15 km from each other. direct amplification, cloning and sequencing of fungal its rrna from the fine roots resulted in 134 high-quality sequences, representing 31 fungal ta ...201424402365
chiral phosphinate degradation by the fusarium species: scope and limitation of the process.biodegradable capacities of fungal strains of fusarium oxysporum (dsmz 2018) and fusarium culmorum (dsmz 1094) were tested towards racemic mixture of chiral 2-hydroxy-2-(ethoxyphenylphosphinyl) acetic acid-a compound with two stereogenic centres. the effectiveness of decomposition was dependent on external factors such as temperature and time of the process. optimal conditions of complete mineralization were established. both fusarium species were able to biodegrade every isomer of tested compou ...201324324893
transposition of the miniature inverted-repeat transposable element mimp1 in the wheat pathogen fusarium culmorum.high-throughput methods are needed for functional genomics analysis in fusarium culmorum, the cause of crown and foot rot on wheat and a type b trichothecene producer. our aim was to develop and test the efficacy of a double-component system based on the ability of the impala transposase to transactivate the miniature inverted-repeat transposable element mimp1 of fusarium oxysporum. we report, for the first time, the application of a tagging system based on a heterologous transposon and of splin ...201222897438
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