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2,4-di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated lactococcus sp.the volatile organic compound 2,4-di-tert-butyl phenol (2,4 dtbp) was purified from the cell free supernatant of a newly isolated lactococcus sp. by solvent extraction and chromatographic techniques. molecular characterization of the compound by esi-ms, (1)h nmr and ftir analysis revealed the structure, c14h22o. fungicidal activity was demonstrated against aspergillus niger, fusarium oxysporum and penicillium chrysogenum by disc diffusion assay. among the cell lines tested for cytotoxicity of th ...201526164257
antimicrobial activity and chemical composition of the essential oils of portuguese foeniculum vulgare fruits.the aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of portugal. composition of essential oils was established by gas chromatography-flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms) analysis. the obtained yields of the essential oils were found to vary greatly in the range ...201525973507
chemical composition and antifungal activity of aaronsohnia pubescens essential oil from algeria.in the present study, the chemical composition of the essential oil obtained from the aerial parts of aaronsohnia pubescens (desf.) k.bremer & humphries (asteraceae), a chamomile-like medicinal plant, was studied. using both gc-ms and gc-fid methods, 58 volatile compounds could be identified representing 96.6% of the total essential oil composition. the main compounds in the essential oil were monoterpene hydrocarbons such as (z)-β-ocimene (53.8%), myrcene (15.2%) and α-pinene (7.7%). moreover, ...201525920238
studies on antimicrobial effects of four ligands and their transition metal complexes with 8-mercaptoquinoline and pyridine terminal groups.four types of ligands (q1-q4) and their complexes (1-36) with transition metal ions have been synthesized, in which two new complexes (15 and 20) have been prepared and tested. in vitro antimicrobial activities of the ligands and their complexes were investigated against a representative panel of strains including two gram positive bacteria (sarcina ureae, staphylococcus aureus), two gram negative bacteria (escherichia coli, pseudomonas aeruginosa) and three fungi (aspergillus niger, saccharomyc ...201525791454
efficacy of oryza sativa husk and quercus phillyraeoides extracts for the in vitro and in vivo control of fungal rot disease of white yam (dioscorea rotundata poir).tuber rot disease is a major constraint to white yam (dioscorea rotundata) production, accounting for 50-60% of annual yield losses in nigeria. the main method of control using synthetic fungicides is being discouraged due to human and environmental health hazards. the potential of oryza sativa husk (osh) and quercus phillyraeoides (qp) extracts for the in vitro and in vivo control of six virulent rot-causing fungal pathogens, lasiodiplodia theobromae, aspergillus niger, rhizoctonia solani, peni ...201425674452
in-vitro antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinone isolated from aegle marmelos (linn.) correa.plant metabolites have wide applications and have the potential to cure different diseases caused by microorganisms. the aim of the study was to evaluate the antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinine isolated from aegle marmelos (linn.) correa.201425359605
application of silica nanoparticles in maize to enhance fungal resistance.in this study, maize treated with nanosilica (20-40 nm) is screened for resistance against phytopathogens such as fusarium oxysporum and aspergillus niger and compared with that of bulk silica. the resistivity is measured for disease index and expression of plant responsive compounds such as total phenols, phenylalanine ammonia lyase, peroxidase and polyphenol oxidase. the results indicate that nanosilica-treated plant shows a higher expression of phenolic compounds (2056 and 743 mg/ml) and a lo ...201425082220
influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities.the results given in the literature are conflicting when considering the relationship between antimicrobial activity and chitosan characteristics. to be able to clarify, we prepared fifteen homogeneous chitosans with different acetylation degrees (da) and molecular weights (mw) by reacetylation of a fully deacetylated chitin under homogeneous conditions. they were tested at different ph values for their antimicrobial activities against four gram-negative bacteria (escherichia coli, pseudomonas a ...201424929684
control of spoilage fungi by protective lactic acid bacteria displaying probiotic properties.thirty-six lactic acid bacteria belong to lactococcus, lactobacillus, enterococcus, and pediococcus were isolated, and the spectrum of antifungal activity was verified against fusarium oxysporum (kacc 42109), aspergillus niger (kacc 42589), fusarium moniliforme (kacc 08141), penicillium chrysogenum (nii 08137), and the yeast candida albicans (mtcc 3017). three isolates, identified as pediococcus pentosaceus (tg2), lactobacillus casei (dy2), and lactococcus (bsn) were selected further, and their ...201424532445
antimicrobial hyperbranched poly(ester amide)/polyaniline nanofiber modified montmorillonite nanocomposites.there has been growing interest in the use of nanomaterials featuring potent of antimicrobial activity in the biomedical domain. it still remains a challenge for the researchers to develop an efficient nanocomposite possessing antimicrobial efficacy against broad spectrum microbes including bacteria, fungi as well as algal consortium, posing serious challenges for the human survival. in addressing the above problem, we report the fabrication of bio-based hyperbranched poly(ester amide) (hbpea)/p ...201424411352
antifungal and antioxidant pyrrole derivative from piper pedicellatum.in continuation of our search for efficient pest control natural products from the flora of the south eastern sub-himalayan biodiversity region, we have investigated wild edible piper pedicellatum c. dc (piperaceae) from arunachal pradesh, india against five important plant pathogenic fungi through an activity guided method, and a new compound, pedicellamide, was isolated. the structure was determined on the basis of extensive spectroscopic studies and confirmed by x-ray crystallography. the com ...201324354199
antifungal cyclic lipopeptides from bacillus amyloliquefaciens strain bo5a.a bioassay-guided fractionation of bacillus amyloliquefaciens strain bo5a afforded the isolation of two new cyclic lipopeptides (1 and 2) as the major lipid constituents (>60%) of the chcl3-meoh (2:1) extract. the chemical structures of the isolated metabolites were elucidated by spectroscopic methods, including 1d and 2d nmr spectroscopy, mass spectrometry (ms), secondary ion mass spectrometry (ms1, ms2), and chemical degradation. the compounds are members of the surfactins family and are based ...201324164115
preparative thin-layer chromatographic separation followed by identification of antifungal compound in cassia laevigata by rp-hplc and gc-ms.several species of the genus cassia are known for their antioxidant, antimicrobial and antidiabetic activities, but some of the lesser-known cassia species, e.g. c. renigera, c. biflora and c. laevigata have not been studied for their biological activities.201423749313
antifungal activity and cytotoxicity of isolated compounds from leaves of breonadia salicina.breonadia salicina is used traditionally to treat wounds, ulcers, fevers, headaches, and fungal infections. the aim of this study was to investigate the antifungal activity of the plant extract and compounds isolated there from.201323727185
antifungal attributes of siderophore produced by the pseudomonas aeruginosa jas-25.siderophores produced by pseudomonas sp. play a vital role in controlling several plant diseases. public concern about chemical pesticides has fostered an interest to seek alternative control strategies. production of siderophore by pseudomonas aeruginosa jas-25 in king b medium was estimated at 400 nm and was partially purified by gel filtration chromatography using sephadex g 25. antifungal activity and inhibition of spore germination was studied against the phytopathogens like fusarium oxyspo ...201423686966
antimicrobial activity of pleurocidin is retained in plc-2, a c-terminal 12-amino acid fragment.an analysis of a series of five peptides composed of various portions of the pleurocidin (plc) sequence identified a l2-amino acid fragment from the c-terminus of plc, designated plc-2, as the smallest fragment that retained a antimicrobial activity comparable to that of the parent compound. mic tests in vitro with low-ionic-strength medium showed that plc-2 has potent activity against pseudomonas aeruginosa, escherichia coli and staphylococcus aureus but not against enterococcus faecalis. the a ...201323603258
a novel and highly efficient method for genetic transformation of fungi employing shock waves.genetic transformation of filamentous fungi is an essential tool in many areas such as biotechnology, medicine, phytopathology and genetics. however, available protocols to transform fungi are inefficient, laborious and have low reproducibility. we report the use of underwater shock waves as a novel method to transform filamentous fungi. an experimental piezoelectric shock wave generator was designed to expose fungal conidia to heterologous dna. the device was successfully tested in aspergillus ...201323583899
synthesis, structural and fungicidal studies of hydrazone based coordination compounds.the coordination compounds of the co(ii), ni(ii) and cu(ii) metal ions derived from imine based ligand, benzil bis(carbohydarzone) were structurally and pharmaceutically studied. the compounds have the general stoichiometry [m(l)]x(2) and [co(l)x(2)], where m=ni(ii) and cu(ii), and x=no(3)(-) and cl(-) ions. the analytical techniques like elemental analyses, molar conductance measurements, magnetic susceptibility measurements, ir, uv/visible, nmr, esi mass and epr were used to study the compound ...201323261608
surface enhanced raman scattering, antibacterial and antifungal active triangular gold nanoparticles.shape controlled syntheses of gold nanoparticles have attracted a great deal of attention as their optical, electronic, magnetic and biological properties are strongly dependent on the size and shape of the particles. here is a report on the surface enhanced raman scattering (sers) activity of cinnamomum zeylanicum leaf broth reduced gold nanoparticles consisting of triangular and spherical like particles, using 2-aminothiophenol (2-atp) and crystal violet (cv) as probe molecules. nanoparticles ...201323220525
biochemical-, biophysical-, and microarray-based antifungal evaluation of the buffer-mediated synthesized nano zinc oxide: an in vivo and in vitro toxicity study.here we describe a simple, novel method of zinc oxide nanoparticle (znp) synthesis and physicochemical characterization. the dose-dependent antifungal effect of znps, compared to that of micronized zinc oxide (mzno), was studied on two pathogenic fungi: aspergillus niger and fusarium oxysporum. superoxide dismutase (sod) activity, ascorbate peroxidase activity, catalase activity, glutathione reductase (gr) activity, thiol content, lipid peroxidation, and proline content in znp-treated fungal sam ...201223163331
design, synthesis, spectral and biological evaluation of novel 1-allyl substituted 2,6-diphenylpiperidin-4-ones and its derivatives of oximes/oxime ethers.a series of 1-allyl-2,6-diphenylpiperidin-4-one oximes 17-24 and 1-allyl-2,6-diphenylpiperidin-4-one o-benzyloximes 25-32 were synthesized from respective 1-allyl-2,6-diphenylpiperidin-4-ones 9-16. the structure and the conformations have been investigated for all the synthesized compounds 9-32 by analytical and spectral techniques (ir, mass, 1h, 13c, 2d nmr and x-ray diffraction). based on the proton nmr analysis, all the synthesized compounds 9-32, exposed chair conformation except compounds 2 ...201223031593
differential chlorate inhibition of chaetomium globosum germination, hyphal growth, and perithecia synthesis.chaetomium globosum kunze:fr is a dermatophytic, dematiaceous fungus that is ubiquitous in soils, grows readily on cellulolytic materials, and is commonly found on water-damaged building materials. chlorate affects nitrogen metabolism in fungi and is used to study compatibility among anamorphic fungi by inducing nit mutants. the effect of chlorate toxicity on c. globosum was investigated by amending a modified malt extract agar (mea), oat agar, and carboxymethyl cellulose agar (cmc) with various ...201222903379
new 1,3,4-bisthiadiazolines: synthesis, characterization and antimicrobial evaluations.the bisthiadiazolines 4a-4g have been synthesized in good yields from the cyclization reactions of bisthiosemicarbazones 3a-3g with acetic anhydride. the condensation reaction of dibenzaldehydes 2a-2g with thiosemicarbazide in alcoholic medium provided 3a-3g and former were obtained from the o-alkylation of 3-hydroxybenzaldehyde with suitable 1,ω-dibromoalkanes under alkaline conditions in the presence of dry etoh/dmf. the intermediates 3a-3g and bishetrocyclics 4a-4g were also screened for thei ...201222832010
bioprospecting micromonospora from kaziranga national park of india and their anti-infective potential.large number of strains was isolated from soils of kaziranga national park of north-east india using selective isolation procedure. they were assigned to the genus micromonospora on the basis of their typical colonial and pigmentation features. the taxonomic identities of the isolates were confirmed on the basis of their molecular characters (16srdna). a total of one hundred micromonospora strains were isolated during the present investigation. the diagnostic cell wall sugar and amino acids were ...201222806196
testing of the safety and the effectiveness of using samjeongpharmacopuncture solution as eye drops.this experimental study was designed to investigate the safety and the effectiveness of samjeong pharmacopuncture solution (sps) manufactured by using a the lowtemperature extract on process.201225780632
genes encoding hevein-like defense peptides in wheat: distribution, evolution, and role in stress response.hevein-like plant defense peptides wamp-1a/b with a unique 10-cys motif are found in the wheat triticum kiharae seeds. three different wamp genomic and cdna sequences were derived from t. kiharae; no introns were spotted in the protein-coding regions of the genes. the deduced wamp precursor proteins consist of a signal peptide, mature peptide (wamp) and c-terminal prosequence. origin of wamps from class i/iv chitinases via deletion of the catalytic domain is proposed based on homology between th ...201222227377
evaluation of certain food additives.this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, including flavouring agents, with a view to concluding as to safety concerns and to preparing specifications for identity and purity. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of and assessment of dietary exposure to food additives, including flavouring agents. a summary follows of the commi ...201223600165
fungal infections among diabetic foot ulcer- patients attending diabetic clinic in kenyatta national hospital, kenya.to isolate and identify fungal pathogens associated with dermatophytoses in diabetic patients and identify the spectrum of yeasts colonising diabetic foot ulcers at kenyatta national hospital.201124968597
molecular characterization of a penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins.we previously described an endo-acting rhamnogalacturonan (rg) lyase, termed pcrgl4a, of penicillium chrysogenum 31b. here, we describe a second rg lyase, called pcrglx. we determined the cdna sequence of the pcrglx gene, which encodes pcrglx. based on analyses using a blast search and a conserved domain search, pcrglx was found to be structurally distinct from known rg lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of nectria haematoco ...201525921806
isolation and expression of enolase gene in fusarium oxysporum f. sp. lycopersici.fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. enolase genes have been isolated from bacteria and fungi, among other organisms. in this research, a large portion of the enolase, eno, gene sequence was isolated from f. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 ...201525349089
sequence analysis and heterologous expression of the wool cuticle-degrading enzyme encoding genes in fusarium oxysporum 26-1.two protease-like proteins, krta and krtc, were identified in fusarium oxysporum 26-1. genes coding these proteins, krta and krtc, were isolated and characterized. recombinant krta (rkrta) and krtc (rkrtc) were successfully expressed in aspergillus oryzae and secreted. the combination of rkrta and rkrtc completely removed the cuticle of wool fibers.201424360406
identification of antifungal niphimycin from streptomyces sp. kp6107 by screening based on adenylate kinase assay.microbial culture extracts are used for natural product screening to find antifungal lead compounds. a microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (ak) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi. a culture extract of streptomyces sp. strain kp6107 lysed cells of fusarium oxysporum f.sp. lycopersici which resulted in high ak activity. the active ingr ...201322915202
degradation of terbuthylazine, difenoconazole and pendimethalin pesticides by selected fungi cultures.contamination of waters by xenobiotic compounds such as pesticides presents a serious environmental problem with substantial levels of pesticides now contaminating european water resources. the aim of this work was to evaluate the ability of the fungi fusarium oxysporum, aspergillus oryzae, lentinula edodes, penicillium brevicompactum and lecanicillium saksenae, for the biodegradation of the pesticides terbuthylazine, difenoconazole and pendimethalin in batch liquid cultures. these pesticides ar ...201222878100
molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia.endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems.201627099679
phytotoxic, antifungal and immunosuppressive metabolites from aspergillus terreus qt122 isolated from the gut of dragonfly.insect gut microbes have been considered as a resource for bioactive metabolites. the aim of this study was to characterize the compounds of a fungus aspergillus terreus qt122 associated with the gut of dragonfly. five main phytotoxic, antifungal, and immunosuppressive substances were isolated from the fungus qt122. the structures of such compounds were identified as emodin (1), 1-methyl emodin (2), terrein (3), methyl 6-acetyl-4-methoxy-7,8-dihydroxynaphthalene-2-carboxylate (4), and dihydrogeo ...201727844127
[suppression of three soil-borne diseases of cucumber by a rhizosphere fungal strain].to understand the effect of rhizosphere fungi on soil-borne diseases of cucumber, 16 fungal, strains from rhizosphere soil were investigated for the antagonistic activity to three soilborne pathogenic fungi with dual culture method and for suppression of cucumber diseases caused by the pathogens in pot experiments. four strains showed antagonism to one or more pathogenic fungi tested. the strain jcl143, identified as aspergillus terreus, showed strong antagonistic activity to the three pathogeni ...201527112016
[using galleria mellonella as an in vivo model to study the virulence of some bacterial and fungal agents].non-vertebrate hosts, such as galleria mellonella, namely wax moth, have been used to study microbial virulence and host defense. this organism has advantages as it is economical, ethically expedient and easy to handle. here we describe an experimental in vivo study using the larvae of galleria mellonella infected with some bacterial and fungal pathogens. in this study, extended-spectrum beta-lactamase (esbl) producing and non-producing escherichia coli, klebsiella pneumoniae and pseudomonas aer ...201526313278
subcutaneous mycoses: an aetiological study of 15 cases in a tertiary care hospital at dibrugarh, assam, northeast india.subcutaneous mycoses are a group of fungal infections of dermis and subcutaneous tissue which consist of sporotrichosis, chromoblastomycosis, phaeohyphomycosis, hyalohyphomycosis, mycetoma, subcutaneous zygomycosis, rhinosporidiosis, lobomycosis and disseminated penicilliosis. a total of 46 consecutive patients with clinically suspected subcutaneous mycoses attending various departments of assam medical college and hospital were included in this prospective study to know the prevalence of subcut ...201525596859
[superficial mycoses in patients from anzoátegui state, venezuela, period 2002-2012].superficial fungal diseases that affect the skin and its appendages are frequently seen in basic triage and in dermatology services. these diseases are distributed in venezuela with an incidence of 92.9%. the aim of this study was to determine the genera and species that cause dermatomycoses in residents of anzoátegui state, venezuela, during the period 2002-2012. a total of 4257 patients with a presumptive diagnosis of superficial mycoses were studied, with ages from 7 months to 79 years. the o ...201425558751
discovery of a linoleate 9s-dioxygenase and an allene oxide synthase in a fusion protein of fusarium oxysporum.fusarium oxysporum is a devastating plant pathogen that oxidizes c₁₈ fatty acids sequentially to jasmonates. the genome codes for putative dioxygenase (dox)-cytochrome p450 (cyp) fusion proteins homologous to linoleate diol synthases (ldss) and the allene oxide synthase (aos) of aspergillus terreus, e.g., foxb_01332. recombinant foxb_01332 oxidized 18:2n-6 to 9s-hydroperoxy-10(e),12(z)-octadecadienoic acid by hydrogen abstraction and antarafacial insertion of molecular oxygen and sequentially to ...201324082064
conversion of fusaric acid to fusarinol by aspergillus tubingensis: a detoxification reaction.the fungus fusarium oxysporum causes wilt diseases of plants and produces a potent phytotoxin fusaric acid (fa), which is also toxic to many microorganisms. an aspergillus tubingensis strain with high tolerance to fa was isolated from soil and designated as cdrat01. hplc analysis of culture filtrates from a. tubingensis isolate cdrat01 grown with the addition of fa indicated the formation of a metabolite over time that was associated with a decrease of fa. spectral analysis and chemical synthesi ...201424352475
fungal endophytes in germinated seeds of the common bean, phaseolus vulgaris.we conducted a survey of fungal endophytes in 582 germinated seeds belonging to 11 colombian cultivars of the common bean (phaseolus vulgaris). the survey yielded 394 endophytic isolates belonging to 42 taxa, as identified by sequence analysis of the ribosomal dna internal transcribed spacer (its) region. aureobasidium pullulans was the dominant endophyte, isolated from 46.7 % of the samples. also common were fusarium oxysporum, xylaria sp., and cladosporium cladosporioides, but found in only 13 ...201627109374
molecular identification of fungal pathogens in nodular skin lesions of cats.in a retrospective study, we investigated 52 formalin-fixed, paraffin-embedded (ffpe) samples from cats with histologically confirmed cutaneous and subcutaneous mycoses to determine if the pathogens could be identified by molecular methods. aim of the study was to obtain a deep understanding of the spectrum of infectious agents, which, as we hypothesized, was not available by histopathology alone. detection of feline and fungal dna was achieved in 92.3% and 94.2% of the samples, respectively. mo ...201525550386
identification and production of azotobacter vinelandii and its antifungal activity against fusarium oxysporum.the phytopathogenic fusarium species are one of the leading causes of loss in agricultural productivity. in search of an efficient bacterial antagonist, 19 soil isolates of azotobacter sp. were screened for antagonistic activity against fusarium oxysporum by agar well diffusion assay. the potential strain was identified as azotobacter vinelandii by 16s rrna sequencing. optimum conditions for culturing a. vinelandii to obtain maximum antifungal activity were determined by varying temperature, ph, ...201324620576
plant growth-promoting endophytic bacteria versus pathogenic infections: an example of bacillus amyloliquefaciens rwl-1 and fusarium oxysporum f. sp. lycopersici in tomato.fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic bacillus amyloliquefaciens rwl-1 against pathogenic fusarium oxysporum f. sp. lycopersici. the results revealed significant suppressio ...201728321368
identification of root-secreted compounds involved in the communication between cucumber, the beneficial bacillus amyloliquefaciens, and the soil-borne pathogen fusarium oxysporum.colonization of plant growth-promoting rhizobacteria (pgpr) is critical for exerting their beneficial effects on the plant. root exudation is a major factor influencing the colonization of both pgpr and soil-borne pathogens within the root system. however, the tripartite interaction of pgpr, plant roots, and soil-borne pathogens is poorly understood. we screened root exudates for signals that mediate tripartite interactions in the rhizosphere. in a split-root system, we found that root colonizat ...201727937752
bacillus species (bt42) isolated from coffea arabica l. rhizosphere antagonizes colletotrichum gloeosporioides and fusarium oxysporum and also exhibits multiple plant growth promoting activity.colletotrichum and fusarium species are among pathogenic fungi widely affecting coffea arabica l., resulting in major yield loss. in the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing colletotrichum gloeosporioides and fusarium oxysporum as well as promotes plant growth.201627863465
molecular characterization of alkaline protease of bacillus amyloliquefaciens sp1 involved in biocontrol of fusarium oxysporum.an alkaline protease gene was amplified from genomic dna of bacillus amyloliquefaciens sp1 which was involved in effective biocontrol of fusarium oxysporum. we investigated the antagonistic capacity of protease of b. amyloliquifaciens sp1, under in vitro conditions. the 5.62 fold purified enzyme with specific activity of 607.69u/mg reported 24.14% growth inhibition of f. oxysporum. however, no antagonistic activity was found after addition of protease inhibitor i.e. pmsf (15mm) to purified enzym ...201627294522
antagonistic activities of novel peptides from bacillus amyloliquefaciens pt14 against fusarium solani and fusarium oxysporum.bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. this paper reports on the antifungal activity of novel peptides isolated from bacillus amyloliquefaciens pt14. reverse-phase high-performance liquid chromatography revealed that b. amyloliquefaciens pt14 produces five peptides (pt14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. in particular, pt14-3 and pt14-4a showed broa ...201526496638
purification and identification of two antifungal cyclic peptides produced by bacillus amyloliquefaciens l-h15.bacillus amyloliquefaciens l-h15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress fusarium oxysporum and other soil-borne fungal plant pathogens. two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. the two compounds were identified by tandem q-tof mass spectroscopy as c15 iturin a (1) and a novel cyclic peptide with a molecular weight of 852.4 da (2). both compounds showed good inhibitory activities ...201526123083
detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of fusarium crown and root rot disease of tomato.to detect and characterize broad-spectrum antipathogen activity of indigenous bacterial isolates obtained from potato soil and soya bean leaves for their potential to be developed as biofungicides to control soilborne diseases such as fusarium crown and root rot of tomato (fcrr) caused by fusarium oxysporum f. sp. radicis-lycopersici (forl).201525512025
isolation of antifungal bacteria from japanese fermented soybeans, natto.an inhibitory effect of a traditional japanese fermented food, natto, was found against plant pathogens such as rhizoctonia solani and fusarium oxysporum, and the bacteria which showed inhibition were isolated from the natto. among isolated bacteria, bc-1 and gac exhibited a strong antagonistic effect in vitro against plant pathogens on an agar medium. the supernatant of bacterial culture also showed strong activity against r. solani, which meant the antimicrobial substances were produced and se ...201325078814
isolation of bacillus amyloliquefaciens s20 and its application in control of eggplant bacterial wilt.bacterial strain s20 was isolated and identified as bacillus amyloliquefaciens based on physiological and biochemical characteristics and a 16s rrna gene sequence analysis. strain s20 inhibits the growth of fusarium oxysporum and ralstonia solanacearum. some genes associated with the synthesis of some lipopeptides were detected in strain s20 by pcr. iturins a were identified as the main antagonistic substrates by analysis with electrospray ionization mass spectrometry/collision-induced dissociat ...201424632400
enhanced rhizosphere colonization of beneficial bacillus amyloliquefaciens sqr9 by pathogen infection.root exudates play important roles in root-soil microorganism interactions and can mediate tripartite interactions of beneficial microorganisms-plant-pathogen in the rhizosphere. however, the roles of organic acid components in this process have not been well studied. in this study the colonization of a plant growth-promoting rhizobacterium, bacillus amyloliquefaciens sqr9, on cucumber root infected by fusarium oxysporum f. sp. cucumerinum j. h. owen (foc) was investigated. chemotaxis and biofil ...201424612247
bacillus amyloliquefaciens q-426 as a potential biocontrol agent against fusarium oxysporum f. sp. spinaciae.in recent years, bacillus species have received considerable attention for the biological control of many fungal diseases. in this study, bacillus amyloliquefaciens q-426 was tested for its potential use against a variety of plant pathogens. our screen for genes involved in the biosynthesis of antifungal agents revealed that the fen and bmy gene clusters are present in the q-426 genome. lipopeptides such as bacillomycin d, fengycin a, and fengycin b were purified from the bacterial culture broth ...201423553741
contribution of bacillomycin d in bacillus amyloliquefaciens sqr9 to antifungal activity and biofilm formation.bacillus amyloliquefaciens strains are capable of suppressing soilborne pathogens through the secretion of an array of lipopeptides and root colonization, and biofilm formation ability is considered a prerequisite for efficient root colonization. in this study, we report that one of the lipopeptide compounds (bacillomycin d) produced by the rhizosphere strain bacillus amyloliquefaciens sqr9 not only plays a vital role in the antagonistic activity against fusarium oxysporum but also affects the e ...201323160135
isolation and molecular characterization of a novel strain of bacillus with antifungal activity from the sorghum rhizosphere.we looked for bacterial strains with antifungal activity in the sorghum rhizosphere. a prescreening procedure to search for hemolytic activity among the isolated strains allowed us to detect good fungitoxic activity in a bacterial isolate that we named um96. this bacterial isolate showed strong growth inhibition in bioassays against the pathogens diaporthe phaseolorum, colletotrichum acutatum, rhizoctonia solani, and fusarium oxysporum. the supernatant of isolate um96 also showed strong hemolyti ...201222869080
antifungal activity of bacillus amyloliquefaciens njn-6 volatile compounds against fusarium oxysporum f. sp. cubense.bacillus amyloliquefaciens njn-6 produces volatile compounds (vocs) that inhibit the growth and spore germination of fusarium oxysporum f. sp. cubense. among the total of 36 volatile compounds detected, 11 compounds completely inhibited fungal growth. the antifungal activity of these compounds suggested that vocs can play important roles over short and long distances in the suppression of fusarium oxysporum.201222685147
the ultrasound-assisted extraction and identification of antifungal substances from b. amyloliquefaciens strain njn-6 suppressing fusarium oxysporum.the primary mechanism underlying antagonism among microorganisms is the production of antagonistic substances called antibiotics that inhibit the growth of pathogens. in this study, the antagonistic substances produced by the bacillus amyloliquefaciens strain njn-6 that had antifungal activity against fusarium oxysporum were extracted and identified. the active antifungal substance was extracted from dried leavening with ultrasound-assisted extraction (uae), using n -butanol as the extractant. h ...201222581589
use of two bacteria for biological control of bayoud disease caused by fusarium oxysporum in date palm (phoenix dactylifera l) seedlings.the bayoud, caused by fusarium oxysporum f. sp. albedinis (foa), is the most destructive disease of date palm (phoenix dactylifera l) in morocco and algeria, with no effective control strategy yet available. in this work, two bacteria, bacillus amyloliquefaciens strain ag1 (ag) and burkholderia cepacia strain cs5 (cs), were examined for their potential to control this disease. both bacterial strains inhibited both growth and sporulation of foa. they released compounds into the culture medium, wh ...201222480991
production of bacillomycin- and macrolactin-type antibiotics by bacillus amyloliquefaciens njn-6 for suppressing soilborne plant pathogens.bacillus amyloliquefaciens strains have been used as biocontrol agents for the suppression of several soilborne plant pathogens. a clearer understanding of the antagonistic mechanisms of action of these bacteria will facilitate their use in the control of plant diseases. antagonistic substances were isolated from the fermentation broth of b. amyloliquefaciens strain njn-6 cultures. these compounds were preconcentrated using an xad-16 column and were purified using reversed-phase high-performance ...201222385216
design, synthesis and antimicrobial activity of 6-n-substituted chitosan derivatives.three novel 6-n-substituted chitosan derivatives were designed and synthesised and characterized by ftir and nmr. the degree of substitution was calculated by elemental analysis results. the antimicrobial activities of the target compounds were evaluated by twofold serial broth dilution method and poisoned food technique. the antifungal activities of 6-aminoethylamino-6-deoxy chitosan (3), 6-butylamino-6-deoxy chitosan (4) and 6-pyridyl-6-deoxy chitosan (5) were significantly increased against r ...201627506558
[effects of continuous application of bio-organic fertilizer on banana production and cultural microflora of bulk soil in orchard with serious disease incidence].a field experiment was conducted for two years to investigate the effects of different fertilization applications on the suppression of banana fusarium wilt disease, crop yield, fruit quality and culturable microflora in a banana orchard which has been monocultured with banana for 12 years and suffered serious banana fusarium wilt disease. the fertilizers included chemical fertilizer (cf), cow manure compost (cm), pig manure compost (pm) and bio-organic fertilizer (bio). the banana soil microflo ...201526094464
activity of flavanones isolated from rhododendron hainanense against plant pathogenic fungi.in a search for naturally occurring antimicrobial compounds in medicinal plants and herbs, seven flavanones were isolated from the aerial parts of rhododendron hainanense and were tested for their antimicrobial activities against six bacteria and six plant pathogenic fungi. within the series of flavanones tested, farrerol (1) displayed moderate antibacterial activities against bacillus cereus, b. subtilis, pseudomonas aeruginosa, staphylococcus aureus, escherichia coli and erwinia carotovora, wi ...201627319130
diversity of indigenous endophytic bacteria associated with the roots of chinese cabbage (brassica campestris l.) cultivars and their antagonism towards pathogens.the study aimed to reveal the diversity of endophytic bacteria in the roots of chinese cabbage (cc) cultivated in two areas in korea, namely, seosang-gun (ss) and haenam-gun (hn), and also in a transgenic plant (tp) from the laboratory. a total of 653 colonies were isolated from the interior of cc roots, comprising 118, 302, and 233 isolates from ss, hn, and tp samples, respectively. based on 16s rrna gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-g+c gram- ...201627095454
antimicrobial activity of ammodaucus leucotrichus fruit oil from algerian sahara.three fruit oil samples of ammodaucus leucotrichus cosson & durieu from algerian sahara were obtained by hydrodistillation and analyzed by gc(ri), gc-ms and 13c nmr spectroscopy. the main compounds were perillaldehyde (87.0-87.9%) and limonene (7.4-8.2%). the antimicrobial effect of the essential oil was evaluated against bacteria, yeasts and filamentous fungi. high antibacterial activity was observed against escherichia coli, staphylococcus aureus. enterobacter cloaceae, bacillus cereus and sal ...201425026729
purification and identification of two antifungal cyclic dipeptides from bacillus cereus subsp. thuringiensis associated with a rhabditid entomopathogenic nematode especially against fusarium oxysporum.the cell-free culture filtrate of bacillus cereus subsp. thuringiensis associated with an entomopathogenic nematode (epn), rhabditis (oscheius) sp., exhibited strong antimicrobial activity. the ethyl acetate extract of the bacterial culture filtrate was purified by silica gel column chromatography to obtain two cyclic dipeptides (cdps). the structure and absolute stereochemistry of this compound were determined based on extensive spectroscopic analyses (fabms, (1)h nmr, (13)c nmr, (1)h-(1)h cosy ...201423402421
[diversity and antimicrobial activities of actinomycetes from pesticide-contaminated spots in shandong peninsula].in order to study diversity and find antimicrobial activities of actinomycetes from pesticide-contaminated spots in shandong peninsula.201222799208
synthesis, spectral studies and biological evaluation of 2-aminonicotinic acid metal complexes.we synthesized 2-aminonicotinic acid (2-ana) complexes with metals such as co(ii), fe(iii), ni(ii), mn(ii), zn(ii), ag(i),cr(iii), cd(ii) and cu(ii) in aqueous media. the complexes were characterized and elucidated using ft-ir, uv-vis, a fluorescence spectrophotometer and thermo gravimetric analysis (tga). tga data showed that the stoichiometry of complexes was 1:2 metal/ligand except for ag(i) and mn(ii) where the ratio was 1:1. the metal complexes showed varied antibacterial, fungicidal and ne ...201626945123
isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of medicago truncatula plants.in the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of medicago truncatula plants. of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10(-2)). hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic dna, yielding 88 haplotypes. the similarity coefficient of nei and li showed a polymorphic diversity ranging from 0.3 to 1. additionally, 8 o ...201425062484
heterologous coexpression of vitreoscilla hemoglobin and bacillus megaterium glucanase in streptomyces lydicus a02 enhanced its production of antifungal metabolites.streptomyces lydicus a02 is a novel producer of commercially important polyene macrocyclic antibiotic natamycin and a potential biocontrol agent to several plant fungal diseases, including wilt caused by fusarium oxysporum f. spp. to improve the natamycin production and the antifungal activity of s. lydicus a02, we coexpressed gene vgb encoding vitreoscilla hemoglobin (vhb) and bglc encoding bacillus megaterium l103 glucanase, both under the control of the strong constitutive erme* promoter, in ...201526453475
antimicrobial constituents from three endophytic fungi.to evaluate the antimicrobial potential of extracts of the endophytic fungi plectophomella sp., physalospora sp., and crataegus monogyna (c. monogyna) and study the tentative identification of their active constituents.201425312126
production and characterization of iturinic lipopeptides as antifungal agents and biosurfactants produced by a marine pinctada martensii-derived bacillus mojavensis b0621a.bacillus mojavensis b0621a was isolated from a pearl oyster pinctada martensii collected from south china sea. while screening for cyclic lipopeptides potentially useful as lead compounds for biological control against soil-bone fungal plant pathogens, three lipopeptides were isolated and purified from the fermentation broth of b. mojavensis b0621a via vacuum flash chromatography coupled with reversed-phase high performance liquid chromatography (rp-hplc). the structural characterization and ide ...201424699814
biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from bacillus pumilus jubch08 displaying antagonism against phytopathogenic fusarium oxysporum.the present investigation highlights the process parameters influencing the submerged fermentation of chitinase by bacillus pumilus jubch08, purification and characterization of the enzyme and determination of antagonistic activity of the bacterium against fusarium oxysporum. medium supplemented with 0.5 % chitin and peptone, at initial ph 8.0, when incubated at 35 °c for 72 h favored highest chitinase production. the enzyme was purified 25.1-fold to homogeneity. the chitinase was found to be th ...201628330157
trans-cinnamic acid and xenorhabdus szentirmaii metabolites synergize the potency of some commercial fungicides.development of novel approaches for the control of fungal phytopathogens is desirable. in this study we hypothesized that the combination of commercial fungicides with certain enhancing agents could result in synergistic levels of control. prior research has indicated that trans-cinnamic-acid (tca), a metabolite of the bacteria photorhabdus luminescens and metabolites of xenorhabdus szentirmaii are particularly toxic to various phytpathogenic fungi when compared to metabolites of other xenorhabd ...201728322849
improved nadph regeneration for fungal cytochrome p450 monooxygenase by co-expressing bacterial glucose dehydrogenase in resting-cell biotransformation of recombinant yeast.fungal cytochrome p450 (cyp) enzymes catalyze versatile monooxygenase reactions and play a major role in fungal adaptations owing to their essential roles in the production avoid metabolites critical for pathogenesis, detoxification of xenobiotics, and exploitation avoid substrates. although fungal cyp-dependent biotransformation for the selective oxidation avoid organic compounds in yeast system is advantageous, it often suffers from a shortage avoid intracellular nadph. in this study, we aimed ...201627666994
diversity and antimicrobial activity of endophytic fungi isolated from cephalotaxus hainanensis li, a well-known medicinal plant in china.about 1051 endophytic fungi were isolated from leaves, branches, barks and stems of cephalotaxus hainanensis li from four sites in hainan, china. the fungi were identified as 21 genera by morphology and its sequences. one dominant species was phomopsis quercella in hainan tropical botanical garden and bawangling nature reserve, with relative frequency of 42·06 and 34·88% respectively. another dominant species was colletotrichum boninense in wuzhishan and jianfengling nature reserves, with relati ...201526280451
rhizosphere inhibition of cucumber fusarium wilt by different surfactin- excreting strains of bacillus subtilis.bacillus subtilis b006 strain effectively suppresses the cucumber fusarium wilt caused by fusarium oxysporum f. sp. cucumerinum (foc). the population dynamics of foc, strain b006 and its surfactin over-producing mutant b841 and surfactin-deficient mutant b1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopeptides excreted by these strains in suppressing foc. results showed that b. subtilis strain b006 effectively suppressed the disease in ...201526060433
control of root rot and wilt diseases of roselle under field conditions.roselle (hibiscus sabdariffa l.) is one of the most important medicinal crops in many parts of the world. in this study, the effects of microelements, antioxidants, and bioagents on fusarium oxysporum, f. solani, and macrophomina phaseolina, the causal pathogens of root rot and wilt diseases in roselle, were examined under field conditions. preliminary studies were carried out in vitro in order to select the most effective members to be used in field control trials. our results showed that micro ...201425606010
plant methyl salicylate induces defense responses in the rhizobacterium bacillus subtilis.bacillus subtilis is a rhizobacterium that promotes plant growth and health. cultivation of b. subtilis with an uprooted weed on solid medium produced pleat-like architectures on colonies near the plant. to test whether plants emit signals that affect b. subtilis colony morphology, we examined the effect of plant-related compounds on colony morphology. bacillus subtilis formed mucoid colonies specifically in response to methyl salicylate, which is a plant-defense signal released in response to p ...201525181478
characterization of volatile constituents from origanum onites and their antifungal and antibacterial activity.essential oils obtained by hydrodistillation (hd) and microwave-assisted hd (mwhd) of origanum onites aerial parts were analyzed by gc and gcims. thirty-one constituents representing 98.6% of the water-distilled oil and 52 constituents representing 99.6% of the microwave-distilled oil were identified. carvacrol (76.8% hd and 79.2% mwhd) and thymol (4.7% hd and 4.4% mwhd) were characterized as major constituents in both essential oils. separation of carvacrol and thymol was achieved by overpressu ...201424645494
combination of endophytic bacillus and beauveria for the management of fusarium wilt and fruit borer in tomato.most of the approaches for biocontrol of pests and diseases have used a single biocontrol agent as antagonist to a single pest or pathogen. this accounts for the inconsistency in the performance of biocontrol agents. the development of a bioformulation possessing a mixture of bioagents could be a viable option for the management of major pests and diseases in crop plants.201424376014
bioassays guided isolation of compounds from chaetomium globosum.the aim of the present study was to evaluate different biological activities of the fungus chaetomium globosum (family chaetomiaceae). the evaluation was done through testing its antimicrobial, antioxidant and anticancer effects. c. globosum was isolated from the cucumber soil (rhizosphere) and caused inhibition of the mycelial growth of fusarium solani, rhizoctonia solani and sclerotium rolfsii in the biculture test. petroleum ether and ethyl acetate extracts of the liquid culture of c. globosu ...201424361402
a novel antifungal protein of bacillus subtilis b25.bacillus subtilis b25 was isolated from banana rhizosphere soil. it has been confirmed for b25 to have stronger antagonism against fusarium oxysporum f.sp.cubense, additionally b25 has good inhibitory to plant pathogens, including corynespora cassiicola, alternaria solani, botrytis cinerea and colletotrichum gloeosporioides on potato dextrose agar (pda) plates. the antagonistic substance can be extracted from cell-free culture broth supernatants by 70% (w/v) (nh4)2 so4 saturation. clear blank ba ...201324255843
identification and characterization of an anti-fungi fusarium oxysporum f. sp. cucumerium protease from the bacillus subtilis strain n7.a newly discovered alkaline antifungal protease named p6 from bacillus subtilis n7 was purified and partially characterized. b. subtilis n7 culture filtrates were purified by 30-60% (nh4)2so4 precipitation, anion-exchange chromatography and gel filtration chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) revealed a single band of 41.38 kda. peptide sequence of protease p6 was determined using a 4800 plus maldi tof/tof™ analyzer system. self-formed adaptor pcr ( ...201323812816
synthesis, antimicrobial evaluation and spectroscopic characterization of novel imidazolone, triazole and triazinone derivatives.the reactions of 2-phenyl-4-arylmethylene-2-oxazolin-5-ones (1a, b) and 2-phenyl-4-arylazo-2-oxazolin-5-ones (8a, b) with p-aminoazobenzene derivatives (2a-c) gave the corresponding imidazolone derivatives (4a-f) and triazole derivatives (10a-f), respectively. also, the reaction of 1a with o-aminophenol to give the imidazolone derivative 5 was studied. the reaction of 1a with 2,4-dinitrophenylhydrazine gave the corresponding 1,2,4-triazine derivatives 14a-c, respectively. the newly synthesized c ...201222796528
biological activities of a mixture of biosurfactant from bacillus subtilis and alkaline lipase from fusarium oxysporum.in this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from bacillus subtilis and an alkaline lipase from fusarium oxysporum (al/bs mix) on several types of microorganisms, as well as their abilities to remove listeria innocua atcc 33093 biofilm from stainless steel coupons. the al/bs mix had a surface tension of around 30 mn.m(-1), indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component. t ...201124031642
isolation, evaluation and characterization of bacillus subtilis from cotton rhizospheric soil with biocontrol activity against fusarium oxysporum.present investigation is based on the isolation of bacillus subtilis from cotton rhizosphere and their evaluation as biocontrol agent against fusarium oxysporum. the production of extracellular hydrolytic enzyme was studied for determining the antagonism. 43% of 21 isolates were identified under the b. subtilis group on the basis of biochemical characterization. 38% isolates showed competitive activity against fusarium oxysporum and exhibit more than 50% mycelial inhibition in dual culture bioas ...201024026922
improved catalytic and antifungal activities of bacillus thuringiensis cells with surface display of chi9602δsp.the cell-surface display of chitinase chi9602δsp was constructed on bacillus thuringiensis bmb171 by using two repeat n-terminal regions of autolysin (mbgn)2 as the anchoring motif. this study aimed to prepare a chitinolytic whole-cell b. thuringiensis biocatalyst with improved catalytic and antifungal activities.201727782360
lipopeptide biosurfactant from bacillus thuringiensis pak2310: a potential antagonist against fusarium oxysporum.the aims of the study were to evaluate the effects of a biosurfactant obtained from a novel bacillus thuringiensis on fusarium oxysporum to determine the morphological changes in the structure of the fungi and its biofilm in the presence of the biosurfactant and to evaluate the toxicity of the biosurfactant on hep-2 human epithelial cell lines.201525456418
heterologous expression of the antifungal β-chitin binding protein cbp24 from bacillus thuringiensis and its synergistic action with bacterial chitinases.the genome sequence analysis of bacillus thuringiensis serovar konkukian s4 has shown to contain two chitinases (chi74, chi39) and two chitin-binding proteins (cbp50 and cbp24). the chi74, chi39 and cbp50 have been characterized previously. the chitin-binding protein cbp24 was cloned and heterologously expressed in escherichia coli. the recombinant protein was purified using a ni-nta purification system. the purified protein was used to study its substrate binding activity using crystalline chit ...201525182053
bacillus thuringiensis strain 199 can induce systemic resistance in tomato against fusarium wilt.the research work was performed to investigate the potential of bacillus thuringiensis strain 199 to induce systemic resistance in tomato against fusarium wilt. roots of two-week-old seedlings of tomato plants were primed with bacterial strain. after 10 days of transplantation, some pots of tomato seedlings were provided with inoculum of fusarium oxysporum lycopersici according to experimental design to induce disease. after 15 days of incubation period, plants challenged with f. oxysporum lycop ...201324294498
phylogenetic diversity of bacterial endophytes of panax notoginseng with antagonistic characteristics towards pathogens of root-rot disease complex.endophytes play an important role in protection of host plants from infection by phytopathogens. endophytic bacteria were isolated from five different parts (root, stem, petiole, leaf and seed) of panax notoginseng and evaluated for antagonistic activity against fusarium oxysporum, ralstonia sp. and meloidogyne hapla, three major pathogens associated with root-rot disease complex of p. notoginseng. from 1000 endophytic bacterial strains evaluated in vitro, 104 strains exhibited antagonistic prop ...201322987248
the antagonistic effect of banana bunchy top virus multifunctional protein b4 against fusarium oxysporum.the viral-induced banana bunchy top disease and the fungal-induced banana blight are two major causes of concern for industrial scale production of bananas. banana blight is particularly troublesome, affecting ∼80% of crops worldwide. strict guidelines and protocols are in place in order to ameliorate the effects of this devastating disease, yet little success has been achieved. from the data presented here, we have found that banana bunchy top virus (bbtv)-infected bananas are more resistant to ...201626369403
impact of diseases on export and smallholder production of banana.banana (musa spp.) is one of the world's most valuable primary agricultural commodities. exported fruit are key commodities in several producing countries yet make up less than 15% of the total annual output of 145 million metric tons (mmt). transnational exporters market fruit of the cavendish cultivars, which are usually produced in large plantations with fixed infrastructures and high inputs of fertilizers, pesticides, and irrigation. in contrast, smallholders grow diverse cultivars, often fo ...201526002290
nematode control related to fusarium wilt in soybean and root rot and zinc deficiency in corn.nematode and disease problems of irrigated, double-cropped soybean and corn, and zinc deficiency of corn were investigated. ethylene dibromide, phenamiphos, and aldicarb were equally effective for controlling nematodes and increasing yields of corn planted minimum-till and soybean planted in a moldboard plow prepared seedbed. the residual effects on yields of nematicides applied to the preceeding crop occurred during 3 years for soybean and 1 year for corn. fusarium wilt symptoms of soybean that ...198519294099
extraction and separation of active ingredients in schisandra chinensis (turcz.) baill and the study of their antifungal effects.schisandra chinensis extracts (ses) have traditionally been used as an oriental medicine for the treatment of various human diseases, however, their further application in the biocontrol of plant disease remains poorly understood. this study was conducted to develop eco-friendly botanical pesticides from extracts of s. chinensis and assess whether they could play a key role in plant disease defense. concentrated active fractions (se-i, se-ii, and se-iii) were obtained from s. chinensis via speci ...201627152614
enhancement of seed dehiscence by seed treatment with talaromyces flavus gg01 and gg04 in ginseng (panax ginseng).seed dehiscence of ginseng (panax ginseng c. a. mayer) is affected by moisture, temperature, storage conditions and microbes. several microbes were isolated from completely dehisced seed coat of ginseng cultivars, chunpoong and younpoong at gumsan, korea. we investigated the potential of five talaromyces flavus isolates from the dehiscence of ginseng seed in four traditional stratification facilities. the isolates showed antagonistic activities against fungal plant pathogens, such as cylindrocar ...201728167883
a new depigmenting-antifungal methylated grindelane from grindelia chiloensis.the new methylated grindelane diterpenoid, 7β-hydroxy-8(17)-dehydrogrindelic acid (1b), together with the known 7α-hydroxy-8(17)-dehydrogrindelic acid (2a), 6-oxogrindelic acid (3a), 4β-hydroxy-6-oxo-19-norgrindelic (4a), 19-hydroxygrindelic acid (5a), 18-hydroxygrindelic acid (6a), 4α-carboxygrindelic acid (7a), 17-hydroxygrindelic acid (8a), 6α-hydroxygrindelic acid (9a), 8,17-bisnor-8-oxagrindelic acid (10a), 7α,8α-epoxygrindelic acid (11a), and strictanonic acid (12a) as methyl esters were o ...201728135782
antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa.plants harbor endophytes with potential bioactivity. markhamia tomentosa (benth) k. schum ex. engl. (bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities.201727937112
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