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the distribution of miniature impala elements and six genes in the fusarium genus is suggestive of horizontal gene transfer.the mimp family of miniature inverted-repeat transposable elements was previously found only in genomes of fusarium oxysporum and is contextually associated with virulence genes in this species. through extensive comparative analysis of 83 f. oxysporum and 52 other fusarium genomes, we uncovered the distribution of different mimp families throughout the genus. we show that (i) mimps are not exclusive to f. oxysporum; (ii) pathogenic isolates generally possess more mimps than non-pathogenic strai ...201728744785
the distribution and host range of the banana fusarium wilt fungus, fusarium oxysporum f. sp. cubense, in asia.fusarium oxysporum formae specialis cubense (foc) is a soil-borne fungus that causes fusarium wilt, which is considered to be the most destructive disease of bananas. the fungus is believed to have evolved with its host in the indo-malayan region, and from there it was spread to other banana-growing areas with infected planting material. the diversity and distribution of foc in asia was investigated. a total of 594 f. oxysporum isolates collected in ten asian countries were identified by vegetat ...201728719631
comparative study on antifungal activities of chitosan nanoparticles and chitosan silver nano composites against fusarium oxysporum species complex.though the metal nanoparticles (nps) have been shown favorable results against fungal diseases, erratic environmental toxicity of nps have raised serious concerns against their applications. hence, it is vital to modify antifungal compounds into safe substitutes over synthetic chemicals. in this study, antifungal effects of chitosan nanoparticles (cnps) and chitosan silver nanocomposites (cagncs) were compared against fusarium oxysporum species complex. cnps and cagncs were synthesized, characte ...201728709896
endophytic fungi harbored in panax notoginseng: diversity and potential as biological control agents against host plant pathogens of root-rot disease.endophytic fungi play an important role in balancing the ecosystem and boosting host growth. in the present study, we investigated the endophytic fungal diversity of healthy panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of p. notoginseng.201728701877
modulation of inter-kingdom communication by phcbsr quorum sensing system in ralstonia solanacearum phylotype i strain gmi1000.ralstonia solanacearum is a ubiquitous soil-borne plant pathogenic bacterium, which frequently encounters and interacts with other soil cohabitants in competition for environmental niches. ralsolamycin, which is encoded by the rmy genes, has been characterized as a novel inter-kingdom interaction signal that induces chlamydospore development in fungi. in this study, we provide the first genetic evidence that the rmy gene expression is controlled by the phcbsr quorum sensing (qs) system in strain ...201728690607
role of fusaric acid in the development of 'fusarium wilt' symptoms in tomato: physiological, biochemical and proteomic perspectives.fusarium wilt is one of the most prevalent and damaging diseases of tomato. among various toxins secreted by the fusarium oxysporum f. sp. lycopersici (causal agent of fusarium wilt of tomato), fusaric acid (fa) is suspected to be a potent pathogenicity factor in tomato wilt disease development. with this rationale the present study was carried out with physiological, biochemical and proteomic perspectives. treatment of fa was given to the leaves of tomato directly through infiltration to show t ...201728683401
host-induced silencing of pathogenicity genes enhances resistance to fusarium oxysporum wilt in tomato.this study presents a novel approach of controlling vascular wilt in tomato by rnai expression directed to pathogenicity genes of fusarium oxysporum f. sp. lycopersici. vascular wilt of tomato caused by fusarium oxysporum f. sp. lycopersici leads to qualitative and quantitative loss of the crop. limitation in the existing control measures necessitates the development of alternative strategies to increase resistance in the plants against pathogens. recent findings paved way to rnai, as a promisin ...201728674943
antifungal activity and expression patterns of extracellular chitinase and β-1,3-glucanase in wickerhamomyces anomalus eg2 treated with chitin and glucan.in this study, the expression patterns of extracellular chitinase and β-1,3-glucanase from cultured wickerhamomyces anomalus eg2 treated with chitin, glucan, and chemical chitinase inhibitors (kinetin, caffeine, and acetazolamide) were investigated using sds-page. relationship between enzyme expression and antifungal activity from yeast plays a very important role for biocontrol of phytopathoges. to determine antifungal activity against phytopathogens, w. anomalus eg2 was shown to strongly inhib ...201728668604
genetic diversity and antifungal susceptibility of fusarium isolates in onychomycosis.fusarium species have emerged as an important human pathogen in skin disease, onychomycosis, keratitis and invasive disease. onychomycosis caused by fusarium spp. the infection has been increasingly described in the immunocompetent and immunosuppressed hosts. considering onychomycosis is a difficult to treat infection, and little is known about the genetic variability and susceptibility pattern of fusarium spp., further studies are necessary to understand the pathogenesis and better to define th ...201728657120
novel loop-mediated isothermal amplification (lamp) assay with a universal qprobe can detect snps determining races in plant pathogenic fungi.tomato wilt pathogen fusarium oxysporum f. sp. lycopersici (fol) is grouped into three races based on their pathogenicity to different host cultivars. rapid detection and discrimination of fol races in field soils is important to prevent tomato wilt disease. although five types of point mutations in secreted in xylem 3 (six3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. the aim of this study is t ...201728652587
an evaluation method for suppression of pathogenic fusarium oxysporum by soil microorganisms using the dilution plate technique.volume 31, no. 3, page 307-313, 2016page 310, legend for fig. 4 incorrectfig. 4. growth degree of fusarium oxysporum f. sp. spinaciae for organic fertilizers at each dilution based on an estimation of the ellipse area (a) and extension length (b) of the colony. cont, compound inorganic fertilizer; sbm, steamed bone meal; cdc, cow dung compost; mi, microbial inoculant. values show mean of medians of degrees with se (n=3). the ellipse area and extension length for control plates were 4,475 mm(2) a ...201728652549
a meta-analysis of quantitative trait loci for abiotic and biotic stress resistance in tetraploid cotton.the number and location of mapped quantitative trait loci (qtl) depend on genetic populations and testing environments. the identification of consistent qtl across genetic backgrounds and environments is a pre-requisite to marker-assisted selection. this study analyzed a total of 661 abiotic and biotic stress resistance qtl based on our previous work and other publications using the meta-analysis software biomercator. it identified chromosomal regions containing qtl clusters for different resist ...201728647758
identification of a potential isr determinant from pseudomonas aeruginosa pm12 against fusarium wilt in tomato.biocontrol of plant diseases through induction of systemic resistance is an environmental friendly substitute to chemicals in crop protection measures. different biotic and abiotic elicitors can trigger the plant for induced resistance. present study was designed to explore the potential of pseudomonas aeruginosa pm12 in inducing systemic resistance in tomato against fusarium wilt. initially the bioactive compound, responsible for isr, was separated and identified from extracellular filtrate of ...201728620396
different mechanisms of trichoderma virens-mediated resistance in tomato against fusarium wilt involve the jasmonic and salicylic acid pathways.in the present study, we investigated the role of trichoderma virens (triv_jsb100) spores or cell-free culture filtrate in the regulation of growth and activation of the defence responses of tomato (solanum lycopersicum) plants against fusarium oxysporum f. sp. lycopersici by the development of a biocontrol-plant-pathogen interaction system. two-week-old tomato seedlings primed with triv_jsb100 spores cultured on barley grains (bgs) or with cell-free culture filtrate (cf) were inoculated with fu ...201728605157
the arabidopsis leucine-rich repeat receptor kinase mik2/lrr-kiss connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses.plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. however, few components involved in plant cell wall integrity sensing have been described to date. using a reverse-genetic approach, we identified the arabidopsis thaliana leucine-rich repeat receptor kinase mik2 as an important regulator of cell wall damage responses triggered upon cellulose biosynthesis inhibition. indeed, loss-of-function mik2 alleles are strongly ...201728604776
fusarium infection causes genotoxic disorders and antioxidant-based damages in orobanche spp.this study aims to evaluate the toxic effects of fusarium oxysporum on root parasitic weed, orobanche spp. comparative genetic and gene expression studies were conducted on uninfected and fungus-infected orobanches. in genetic studies, isolated total dna was amplified by rapd pcr. fragment properties were analysed by gts test. according to the results, the fragment properties of control and fusarium infected (experimental) groups varied widely; and it has been observed that fusarium has genotoxi ...201728602401
in vitro and in vivo antifungal efficacy of plant based lawsone against fusarium oxysporum species complex.fusarium oxysporum is an ascomycete facultative fungus which generally affects to plants. however, it is recently known as a serious emerging opportunistic pathogen of human and other animals. f. oxysporum shows broad resistance to commonly used antifungal agents and therefore development of alternative therapeutic agents is required. in this study, we investigated the antifungal efficacy of plant based natural lawsone against pathogenic f. oxysporum. antifungal susceptibility test determined th ...201728602398
purification, characterization and retting of crotolaria juncea fibres by an alkaline pectin lyase from fusarium oxysporum mtcc 1755.using solid-state fermentation, production of an industrially important pectin lyase from a fungal strain fusarium oxysporum mtcc 1755 was attempted, which was further subjected to purification and characterization. the enzyme was purified by three steps, namely ammonium sulfate fractionation, cation-exchange chromatography on cm cellulose followed by gel filtration chromatography using sephadex g-100 column. a 16-fold purification with 31.2% yield and 3.2 u/mg specific activity was achieved. th ...201728593518
polyketide-terpene hybrid metabolites from an endolichenic fungus pestalotiopsis sp.five new polyketide-terpene hybrid metabolites (1-5) with highly functionalized groups, together with six known derivatives (6-11), were isolated from the endolichenic fungus pestalotiopsis sp. their structures were elucidated by extensive nmr experiments including (1)h, (13)c, hmqc, cosy, and hmbc. the relative configurations of the new compounds were determined by analysis of coupling constants and roesy correlations. the absolute configurations especially the secondary alcohol at c-15 in 1 an ...201728593175
specific pcr detection of fusarium oxysporum f. sp. raphani: a causal agent of fusarium wilt on radish plants.fusarium oxysporum, a causal agent of fusarium wilt, is one of the most important fungal pathogens worldwide, and detection of f. oxysporum dna at the forma specialis level is crucial for disease diagnosis and control. in this study, two novel f. oxysporum f. sp. raphani (for)-specific primer sets were designed, for1-f/for1-r and for2-f/for2-r, to target foqg_17868 and foqg_17869 orfs, respectively, which were selected based on the genome comparison of other formae speciales of f. oxysporum incl ...201728585248
biodiversity in targeted metabolomics analysis of filamentous fungal pathogens by (1)h nmr-based studies.the taxonomical classification among fungi kingdom in the last decades was evolved. in this work the targeted metabolomics study based on (1)h nmr spectroscopy combined with chemometrics tools was reported to be useful for differentiation of three model of fungal strains, which represent various genus of ascomycota (aspergillus pallidofulvus, fusarium oxysporum, geotrichum candidum) were selected in order to perform metabolomics studies. each tested species, revealed specific metabolic profile o ...201728585165
corrigendum: transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.[this corrects the article on p. 362 in vol. 8, pmid: 28367157.].201728572815
an allene oxide and 12-oxophytodienoic acid are key intermediates in jasmonic acid biosynthesis by fusarium oxysporum.fungi can produce jasmonic acid (ja) and its isoleucine conjugate in large quantities, but little is known about the biosynthesis. plants form ja from 18:3n-3 by 13s-lipoxygenase (lox), allene oxide synthase, and allene oxide cyclase. shaking cultures of fusarium oxysporum f. sp. tulipae released over 200 mg of jasmonates per liter. nitrogen powder of the mycelia expressed 10r-dioxygenase-epoxy alcohol synthase activities, which was confirmed by comparison with the recombinant enzyme. the 13s-lo ...201728572515
tobacco rattle virus-based silencing of enoyl-coa reductase gene and its role in resistance against cotton wilt disease.a tobacco rattle virus (trv)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (gossypium hirsutum). this approach was used to investigate the function of the enoyl-coa reductase (ghecr) gene in pathogen defense. amino acid sequence alignment of arabidopsis ecr with homologous sequence from g. hirsutum, g. arboreum, g. herbaceum and g. barbadense showed that ecrs are highly conserved among these species. trv-based silencing of gh ...201728567686
illumina-based sequencing analysis directed selection for actinobacterial probiotic candidates for banana plants.as potential probiotic candidates, plant vertically transmitted actinobacteria are beneficial to growth and health of host plants. new methods to isolate the actinobacterial taxa with low growth rates should be developed. based on the actinobacterial population information, the probiotic actinobacterial taxa could be directly isolated from healthy banana shoot tips. however, actinobacterial dnas with high gc contents could bias estimates of actinobacteria by pcr. in the study, two amplicon seque ...201728560514
small rna sequencing for secondary metabolite analysis in persicaria minor.persicaria minor (kesum) is an important medicinal plant and commonly found in southeast countries; malaysia, thailand, indonesia, and vietnam. this plant is enriched with a variety of secondary metabolites (sms), and among these sms, terpenoids are in high abundance. terpenoids are comprised of many valuable biomolecules which have well-established role in agriculture and pharmaceutical industry. in p. minor, for the first time, we have generated small rnas data sets, which can be used as tool ...201728560169
clean up fly ash from coal burning plants by new isolated fungi fusarium oxysporum and penicillium glabrum.in turkey approximately 45 million tons of coals are burned in a year and 19.3 million tons of fly ash have emerged. the bioremediation of heavy metals or different elements from fly ash makes them bio-available. however, in previous studies, requiring of long operational time and failing to show tolerance to high pulp densities of fly ash of selected fungal species makes them impractical. in this work, bioremediation of fly ash by new isolated fungi fusarium oxysporum and penicillium glabrum we ...201728554126
analysis of protein profile of tomato root infected with fusarium oxysporum f. sp lycopersici.fusarium wilt caused by fusarium oxysporum f. sp lycopersici (fol) is one of the main diseases affecting tomatoes. the bhrs 2,3 genotype of tomato is, however, resistant to this disease. a proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. protein was extracted and separated by two-dimensional electrophoresis followed by staining with coomassie brilliant blue. the proteins were ide ...201728549197
transcriptomic dissection reveals wide spread differential expression in chickpea during early time points of fusarium oxysporum f. sp. ciceri race 1 attack.plants' reaction to underground microorganisms is complex as sessile nature of plants compels them to prioritize their responses to diverse microorganisms both pathogenic and symbiotic. roots of important crops are directly exposed to diverse microorganisms, but investigations involving root pathogens are significantly less. thus, more studies involving root pathogens and their target crops are necessitated to enrich the understanding of underground interactions. present study reported the molec ...201728542579
characterization of silver nanoparticles produced by biosynthesis mediated by fusarium oxysporum under different processing conditions.the goal of this study was the biosynthesis of silver nanoparticles (snps) mediated by the fungus fusarium oxysporum, as well as the characterization of these nanoparticles including evaluation of the particles size and stability under different processing conditions. the results showed that the biosynthesis produced silver nanoparticles having a mean size of 34 nm and zeta potential values below -30 mv at the conditions used, characterizing the nanoparticles as being stable in suspension. ultra ...201728540439
detoxification of fusaric acid by the soil microbe mucor rouxii.fusarium oxysporum f. sp. vasinfectum race 4 (vcg0114), which causes root rot and wilt of cotton (gossypium hirsutum and g. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the san joaquin valley of california. this pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of f. oxysporum f. sp. vasinfectum (fov) that are indigenous to the united states. fusaric a ...201728538103
wble2 transcription factor in streptomyces griseus s4-7 plays an important role in plant protection.streptomyces griseus s4-7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for fusarium wilt suppressive soils. s. griseus s4-7 shows specific and pronounced antifungal activity against fusarium oxysporum f. sp. fragariae. in the streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wble2 function as a regulator has emerged as a new group in whi transcription ...201728523731
non-edible oil cakes as a novel substrate for dpa production and augmenting biocontrol activity of paecilomyces variotii.the present study investigated the use of waste non-edible oil cakes (jatropha, karanja, neem, and mahua) as a substrate for the growth of paecilomyces variotii and dipicolinic acid (dpa) production. previous researches proved the efficacy of dpa in suppressing certain pathogens that are deleterious to the plants in the rhizosphere. dpa production was statistical optimized by amending non-edible oil cakes in growing media as nitrogen and sugars (dextrose, glucose, and lactose) as carbon source. ...201728512455
purification and site-directed mutagenesis of linoleate 9s-dioxygenase-allene oxide synthase of fusarium oxysporum confirms the oxygenation mechanism.plants and fungi form jasmonic acid from α-linolenic acid. the first two steps of biosynthesis in plants occur by sequential transformation by 13s-lipoxygenase and allene oxide synthase (aos). the biosynthesis in fungi may follow this classical scheme, but the only fungal aos discovered so far are cytochromes p450 (cyp) fused to 8- and 9-dioxygenases (dox). in the present report, we purified recombinant 9s-dox-aos of fusarium oxysporum from cell lysate by cobalt affinity chromatography to near h ...201728502466
effectiveness of a full-scale horizontal slow sand filter for controlling phytopathogens in recirculating hydroponics: from microbial isolation to full microbiome assessment.the microbial disinfestation efficiency of an innovative horizontal-flow slow sand filter (hssf) for treating nutrient solution spent from an experimental closed-loop nursery was evaluated by means of a combination of culture-dependent and independent molecular techniques. a dense inoculum of the fungal plant pathogen fusarium oxysporum f.sp. lycopersici was applied in the fertigation system (10(6) cells per ml). indigenous and introduced populations of eubacteria and fungi were assessed in the ...201728499226
fusarium oxysporum and the fusarium wilt syndrome.the fusarium oxysporum species complex (fosc) comprises a multitude of strains that cause vascular wilt diseases of economically important crops throughout the world. although sexual reproduction is unknown in the fosc, horizontal gene transfer may contribute to the observed diversity in pathogenic strains. development of disease in a susceptible crop requires f. oxysporum to advance through a series of transitions, beginning with spore germination and culminating with establishment of a systemi ...201728489498
the endochitinase vdech from verticillium dahliae inhibits spore germination and activates plant defense responses.chitinases function in the digestion of chitin molecules, which are present principally in insects and fungi. in plants, chitinase genes play important roles in defense, and their expression can be triggered in response to both biotic and abiotic stresses. in this study, we cloned and characterized an endochitinase (vdech) from verticillium dahliae, strain vd080. the vdech coding region consists of 1845bp with two exons and one 54bp intron, encoding a 615 amino acid protein with the predicted mo ...201728483050
comparative transcriptomic analysis of race 1 and race 4 of fusarium oxysporum f. sp. cubense induced with different carbon sources.the fungal pathogen fusarium oxysporum f. sp. cubense causes fusarium wilt, one of the most destructive diseases in banana and plantain cultivars. pathogenic race 1 attacks the "gros michel" banana cultivar, and race 4 is pathogenic to the cavendish banana cultivar and those cultivars that are susceptible to foc1. to understand the divergence in gene expression modules between the two races during degradation of the host cell wall, we performed rna sequencing to compare the genome-wide transcrip ...201728468818
genome-wide analysis of autophagy-related genes in banana highlights maatg8s in cell death and autophagy in immune response to fusarium wilt.maatg8s play important roles in hypersensitive-like cell death and immune response, and autophagy is essential for disease resistance against foc in banana. autophagy is responsible for the degradation of damaged cytoplasmic constituents in the lysosomes or vacuoles. although the effects of autophagy have been extensively revealed in model plants, the possible roles of autophagy-related gene in banana remain unknown. in this study, 32 maatgs were identified in the draft genome, and the profiles ...201728451821
mediator18 and mediator20 confer susceptibility to fusarium oxysporum in arabidopsis thaliana.the conserved protein complex known as mediator conveys transcriptional signals by acting as an intermediary between transcription factors and rna polymerase ii. as a result, mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. in this report we identify the head domain subunits mediator18 and mediator20 as important susceptibility factors for fusarium oxysporum infection in arabidopsis thaliana. mutants of med18 and med20 display down- ...201728441405
nonsteroidal anti-inflammatory drugs (nsaids) inhibit the growth and reproduction of chaetomium globosum and other fungi associated with water-damaged buildings.indoor mold due to water damage causes serious human respiratory disorders, and the remediation to homes, schools, and businesses is a major expense. prevention of mold infestation of building materials would reduce health problems and building remediation costs. nonsteroidal anti-inflammatory drugs (nsaids) inhibit yeasts and a limited number of filamentous fungi. the purpose of this research was to determine the possible inhibitory activity of nonsteroidal anti-inflammatory drugs (nsaids) on g ...201728795317
chemical composition and antifungal activity of hedera helix leaf ethanolic extract.the 50% ethanol extract obtained from hedera helix leaves was investigated regarding the presence and quantity of polyphenols, sterols and in vitro antifungal activity against phytopathogenic fungi. the chemical analysis revealed the presence of rutin, quercetin and kaempferol in the non-hydrolysed sample and quercetin and kaempferol in the hydrolysed sample and stigmasterol in the ivy leaf extract (nonhydrolysed sample). the antifungal activity against phytopathogenic fungi (aspergillus niger, ...201728605982
isolation of a potential biocontrol agent paenibacillus polymyxa nsy50 from vinegar waste compost and its induction of host defense responses against fusarium wilt of cucumber.fusarium wilt caused by fusarium oxysporum f. sp. cucumerinum (foc) is one of the major destructive soil-borne diseases infecting cucumber. in this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. the 16s rdna gene demonstrated that the biocontrol agents were paenibacillus polymyxa (p. polymyxa), bacillus amyloliquefaciens (b. amyloliquefaciens) and bacillus ...201728647117
in vitro antimicrobial activities and mechanism of 1-octen-3-ol against food-related bacteria and pathogenic fungi.1-octen-3-ol, known as mushroom alcohol, is a natural product extracted from fungi and plants. its antimicrobial activities against five common food-related bacteria and two pathogenic fungi were evaluated in this paper, including staphylococcus aureus, bacillus subtilis, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, fusarium tricinctum and fusarium oxysporum. the results showed that 1-octen-3-ol had a strong antibacterial activity against the tested bacteria, especially ...201728794307
c-coordinated o-carboxymethyl chitosan metal complexes: synthesis, characterization and antifungal efficacy.a novel type of o-carboxymethyl chitosan schiff bases (o-cspx) was synthesized via a condensation reaction. after the coordination reaction of cupric ions, zinc ions and nickel ions, metal complexes (o-cspx-m) were achieved. the theoretical structure of o-cspx-m calculated by gaussian 09 reveals that the copper ions and nickel ions underwent dsp(2) hybridization, the zinc ions underwent sp(3) hybridization, and they all coordinated by the carbon atom in the p-π conjugate group. then, the structu ...201728774807
disease resistance to multiple fungal and oomycete pathogens evaluated using a recombinant inbred line population in pepper.incorporating disease resistance into cultivars is a primary focus of modern breeding programs. resistance to pathogens is often introgressed from landrace or wild individuals with poor fruit quality into commercial-quality cultivars. sites of multiple disease resistance (mdr) are regions or "hotspots" of the genome with closely linked genes for resistance to different pathogens that could enable rapid incorporation of resistance. an f2-derived f6 recombinant inbred line population from a cross ...201728762287
elucidation of the biosynthetic pathway of cis-jasmone in lasiodiplodia theobromae.in plants, cis-jasmone (cj) is synthesized from α-linolenic acid (la) via two biosynthetic pathways using jasmonic acid (ja) and iso-12-oxo-phytodienoic acid (iso-opda) as key intermediates. however, there have been no reports documenting cj production by microorganisms. in the present study, the production of fungal-derived cj by lasiodiplodia theobromae was observed for the first time, although this production was not observed for botrytis cinerea, verticillium longisporum, fusarium oxysporum, ...201728751737
production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source.the aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi (wrf) using peels or discarded potato as the sole nutrient source.201728718996
the antibiotic polymyxin b exhibits novel antifungal activity against fusarium species.the genus fusarium comprises many species, including fusarium oxysporum, fusarium solani, fusarium graminearum and fusarium verticillioides, and causes severe infections in plants and humans. in clinical settings, fusarium is the third most frequent mould to cause invasive fungal infections after aspergillus and the mucorales. f. solani and f. oxysporum are the most prevalent fusarium spp. causing clinical disease. however, few effective antifungal drugs are available to treat human and plant fu ...201728433743
antimicrobial textiles: biogenic silver nanoparticles against candida and xanthomonas.this paper introduces cotton fibers impregnated with biogenic silver nanoparticles (agnps), synthesized from a fusarium oxysporum fungal filtrate (ff) solution, and open up the possibility for their use in medical environment and agriculture clothing as means to avoid microbial spreading. after thorough agnps characterization, regarding their physical, chemical and biochemical properties, minimum inhibitory concentrations (mic) against some human and orange tree pathogens were determined. we rep ...201728415502
fungal endophytes isolated from protium heptaphyllum and trattinnickia rhoifolia as antagonists of fusarium oxysporum.control of fungal pathogens is mainly addressed by the use of chemically synthesized fungicides which result in environmental pollution, developing resistance after prolonged use. in this context, endophytes have been recognized as potential biocontrollers, and also as a promising source of antifungal metabolites. therefore, as part of our research on phytopathogen controllers, 355 fungal endophytes were isolated from protium heptaphyllum and trattinnickia rhoifolia (burseraceae), both ethnobota ...201728495036
evaluation of multifarious plant growth promoting traits, antagonistic potential and phylogenetic affiliation of rhizobacteria associated with commercial tea plants grown in darjeeling, india.plant growth promoting rhizobacteria (pgpr) are studied in different agricultural crops but the interaction of pgpr of tea crop is not yet studied well. in the present study, the indigenous tea rhizobacteria were isolated from seven tea estates of darjeeling located in west bengal, india. a total of 150 rhizobacterial isolates were screened for antagonistic activity against six different fungal pathogens i.e. nigrospora sphaerica (kj767520), pestalotiopsis theae (itcc 6599), curvularia eragostid ...201728771547
production, characterization, and antifungal activity of a biosurfactant produced by rhodotorula babjevae ys3.sophorolipids are one of the most promising glycolipid biosurfactants and have been successfully employed in bioremediation and various other industrial sectors. they have also been described to exhibit antimicrobial activity against different bacterial species. nevertheless, previous literature pertaining to the antifungal activity of sophorolipids are limited indicating the need for further research to explore novel strains with wide antimicrobial activity. a novel yeast strain, rhodotorula ba ...201728558761
the powdery mildew-resistant arabidopsis mlo2 mlo6 mlo12 triple mutant displays altered infection phenotypes with diverse types of phytopathogens.arabidopsis thaliana mlo2 mlo6 mlo12 triple mutant plants exhibit complete immunity against infection by otherwise virulent obligate biotrophic powdery mildew fungi such as golovinomyces orontii. while this phenotype is well documented, the interaction profile of the triple mutant with other microbes is underexplored and incomplete. here, we thoroughly assessed and quantified the infection phenotypes of two independent powdery mildew-resistant triple mutant lines with a range of microbes. these ...201728839137
preparation and characterization of novel cationic chitosan derivatives bearing quaternary ammonium and phosphonium salts and assessment of their antifungal properties.chitosan is an abundant and renewable polysaccharide, its derivatives exhibit attractive bioactivities and the wide applications in various biomedical fields. in this paper, two novel cationic chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized via trimethylation, chloride acetylation, and quaternization with tricyclohexylphosphine and triphenylphosphine. the structures and properties of synthesized products in the reactions were characterized by ftir sp ...201728858241
design, synthesis of novel chitosan derivatives bearing quaternary phosphonium salts and evaluation of antifungal activity.two novel chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized, including tricyclohexylphosphonium acetyl chitosan chloride (tcpacsc) and triphenylphosphonium acetyl chitosan chloride (tppacsc), and characterized by ftir, (1)h nmr, and (13)c nmr spectra. the degree of substitution was also calculated by elemental analysis results. their antifungal activities against colletotrichum lagenarium, watermelon fusarium, and fusarium oxysporum were investigated i ...201728445700
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds.natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars.201728480436
a novel α-galactosidase from fusarium oxysporum and its application in determining the structure of the gum arabic side chain.we previously reported that fusarium oxysporum 12s produces two bifunctional proteins, foap1 and foap2, with α-d-galactopyranosidase (gpase) and β-l-arabinopyranosidase (apase) activities. the aim of this paper was to purify a third gpase, fogp1, from culture supernatant of f. oxysporum 12s, to characterize it, and to determine its mode of action towards gum arabic. a cdna encoding fogp1 was cloned and the protein was overexpressed in escherichia coli. module sequence analysis revealed the prese ...201728554382
structure-function analysis of the fusarium oxysporum avr2 effector allows uncoupling of its immune-suppressing activity from recognition.plant pathogens employ effector proteins to manipulate their hosts. fusarium oxysporum f. sp. lycopersici (fol), the causal agent of tomato wilt disease, produces effector protein avr2. besides being a virulence factor, avr2 triggers immunity in i-2 carrying tomato (solanum lycopersicum). fol strains that evade i-2 recognition carry point mutations in avr2 (e.g. avr2(r45h) ), but retain full virulence. here we investigate the virulence function of avr2 and determine its crystal structure. transg ...201728857169
a mobile pathogenicity chromosome in fusarium oxysporum for infection of multiple cucurbit species.the genome of fusarium oxysporum (fo) consists of a set of eleven 'core' chromosomes, shared by most strains and responsible for housekeeping, and one or several accessory chromosomes. we sequenced a strain of fo f.sp. radicis-cucumerinum (forc) using pacbio smrt sequencing. all but one of the core chromosomes were assembled into single contigs, and a chromosome that shows all the hallmarks of a pathogenicity chromosome comprised two contigs. a central part of this chromosome contains all identi ...201728831051
comparison of transcriptome profiles by fusarium oxysporum inoculation between fusarium yellows resistant and susceptible lines in brassica rapa l.resistant and susceptible lines in brassica rapa have different immune responses against fusarium oxysporum inoculation. fusarium yellows caused by fusarium oxysporum f. sp. conglutinans (foc) is an important disease of brassicaceae; however, the mechanism of how host plants respond to foc is still unknown. by comparing with and without foc inoculation in both resistant and susceptible lines of chinese cabbage (brassica rapa var. pekinensis), we identified differentially expressed genes (degs) b ...201728819684
pathological interrelations of soil-borne diseases in cucurbits caused by fusarium species and meloidogyne incognita.pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by fusarium isolates (fi) and the root-knot nematode (rkn), meloidogyne incognita were characterized by the fusarium disease severity index (di), rkn gall index (gi) and eggmass index (ei) in inoculation tests using fi and rkn. virulence of fi as determined by di at 4 weeks after inoculation was mostly in the higher order of fusarium proliferatum f6, f5 and fusarium oxys ...201728811758
investigating the diversity of effector genes in the banana pathogen, fusarium oxysporum f.sp. cubense, reveals evidence of horizontal gene transfer.it is hypothesised that the virulence of phytopathogenic fungi is mediated through the secretion of small effector proteins that interfere with the defence responses of the host plant. in fusarium oxysporum, one family of effectors, the secreted in xylem (six) genes, have been identified. we sought to characterise the diversity and evolution of the six genes in the banana-infecting lineages of f. oxysporum f.sp. cubense (foc). whole-genome sequencing data was generated for the 23 genetic lineage ...201728802020
chickpea-fusarium oxysporum interaction transcriptome reveals differential modulation of plant defense strategies.fusarium wilt is one of the major biotic stresses reducing chickpea productivity. the use of wilt-resistant cultivars is the most appropriate means to combat the disease and secure productivity. as a step towards understanding the molecular basis of wilt resistance in chickpea, we investigated the transcriptomes of wilt-susceptible and wilt-resistant cultivars under both fusarium oxysporum f.sp. ciceri (foc) challenged and unchallenged conditions. transcriptome profiling using longsage provided ...201728798320
synthesis, characterization and antifungal activity of coumarin-functionalized chitosan derivatives.four novel coumarin-functionalized chitosan derivatives 4a-4d were synthesized via condensation reactions of thiosemicarbazide chitosan with coumarin derivatives. their structures were confirmed by ft-ir, (13)c nmr, xrd and elemental analysis. their antifungal activities against three kinds of phytopathogens, alternaria solani sorauer (a. solani), fusarium oxysporum f.sp. vasinfectum (f. oxysporum) and fusarium moniliforme (f. moniliforme), were tested using the mycelial growth rate in vitro at ...201728797818
rna sequencing reveals that endoplasmic reticulum stress and disruption of membrane integrity underlie dimethyl trisulfide toxicity against fusarium oxysporum f. sp. cubense tropical race 4.fusarium wilt of banana, a destructive disease that affects banana production, is caused by fusarium oxysporum f. sp. cubense tropical race 4 (foc tr4). in a previous study, we confirmed the strong inhibitory effects of chinese leek (allium tuberosum) on the incidence of this disease. sulfur compounds are the primary antifungal constituents of chinese leek. among these, dimethyl trisulfide (dt) was the most abundant and exhibited the strongest inhibition of foc tr4 growth and development. in the ...201728790985
bacterial diseases of bananas and enset: current state of knowledge and integrated approaches toward sustainable management.bacterial diseases of bananas and enset have not received, until recently, an equal amount of attention compared to other major threats to banana production such as the fungal diseases black leaf streak (mycosphaerella fijiensis) and fusarium wilt (fusarium oxysporum f. sp. cubense). however, bacteria cause significant impacts on bananas globally and management practices are not always well known or adopted by farmers. bacterial diseases in bananas and enset can be divided into three groups: (1) ...201728785275
synthesis of cdse quantum dots using fusarium oxysporum.cdse quantum dots are often used in industry as fluorescent materials. in this study, cdse quantum dots were synthesized using fusarium oxysporum. the cadmium and selenium concentration, ph, and temperature for the culture of f. oxysporum (fusarium oxysporum) were optimized for the synthesis, and the cdse quantum dots obtained from the mycelial cells of f. oxysporum were observed by transmission electron microscopy. ultra-thin sections of f. oxysporum showed that the cdse quantum dots were preci ...201628773975
interdigital intertrigo due to fusarium oxysporum.fusariosis is a fungal infection often involving the skin. various species can cause local, focally invasive, or disseminated infections. the routes of entry for fusarium species include the respiratory tract, gastrointestinal tract, toe nails, trauma to the skin, and indwelling central venous catheter.201628681012
antioxidant and antifungal activity of selected medicinal plant extracts against phytopathogenic fungi.medicinal plants are used by many ethnic groups as a source of medicine for the treatment of various ailments in both humans and domestic animals. these plants produce secondary metabolites that have antimicrobial properties, thus screening of medicinal plants provide another alternative for producing chemical fungicides that are relatively non-toxic and cost-effective.201628852739
application of serratia marcescens rz-21 significantly enhances peanut yield and remediates continuously cropped peanut soil.continuous cropping practices cause a severe decline in peanut yield. the aim of this study was to investigate the remediation effect of serratia marcescens on continuously cropped peanut soil. a pot experiment was conducted under natural conditions to determine peanut agronomic indices, soil microorganism characteristics, soil enzyme activities and antagonism ability to typical pathogens at different growth stages. four treatments were applied to red soil as follows: an active fermentation liqu ...201625640613
combined action of the major secreted exo- and endopolygalacturonases is required for full virulence of fusarium oxysporum.the genome of the tomato pathogen fusarium oxysporum f. sp. lycopersici encodes eight different polygalacturonases (pgs): four endopgs and four exopgs. quantitative real-time reverse transcription-polymerase chain reaction (rt-pcr) revealed that endopgs pg1 and pg5 and exopgs pgx4 and pgx6 are expressed at significant levels during growth on citrus pectin, polygalacturonic acid or the monomer galacturonic acid, as well as during the infection of tomato plants. the remaining pg genes exhibit low ...201626060046
the neutral metallopeptidase nmp1 of trichoderma guizhouense is required for mycotrophy and self-defence.trichoderma guizhouense njau 4742 (harzianum clade) can suppress the causative agent of banana wild disease fusarium oxysporum f. sp. cubense 4 (foc4). to identify genes involved in this trait, we used t-dna insertional mutagenesis and isolated one mutant that was unable to overgrow foc4 and had reduced antifungal ability. using the high-efficiency thermal asymmetric interlaced-pcr, the t-dna was located in the terminator of a neutral metalloprotease gene (encoding a merops family m35 protease), ...201626118314
microbial and biochemical basis of a fusarium wilt-suppressive soil.crops lack genetic resistance to most necrotrophic pathogens. to compensate for this disadvantage, plants recruit antagonistic members of the soil microbiome to defend their roots against pathogens and other pests. the best examples of this microbially based defense of roots are observed in disease-suppressive soils in which suppressiveness is induced by continuously growing crops that are susceptible to a pathogen, but the molecular basis of most is poorly understood. here we report the microbi ...201626057845
comparative genomics of fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the fom-2 resistance gene in melon.development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. these proteins secreted by pathogens are called 'avirulence' proteins. their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. we have used genome sequ ...201626305378
detailed mapping of a resistance locus against fusarium wilt in cultivated eggplant (solanum melongena).this is the first report on genetic mapping of a resistance locus against fusarium wilt caused by the plant pathogen fusarium oxysporum f. sp. melongenae in cultivated eggplant.201626582508
identification of i-7 expands the repertoire of genes for resistance to fusarium wilt in tomato to three resistance gene classes.the tomato i-3 and i-7 genes confer resistance to fusarium oxysporum f. sp. lycopersici (fol) race 3 and were introgressed into the cultivated tomato, solanum lycopersicum, from the wild relative solanum pennellii. i-3 has been identified previously on chromosome 7 and encodes an s-receptor-like kinase, but little is known about i-7. molecular markers have been developed for the marker-assisted breeding of i-3, but none are available for i-7. we used an rna-seq and single nucleotide polymorphism ...201626177154
photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi fusarium oxysporum, fusarium moniliforme and fusarium solani.the search for alternatives to control microorganisms is necessary both in clinical and agricultural areas. antimicrobial photodynamic treatment (apdt) is a promising light-based approach that can be used to control both human and plant pathogenic fungi. in the present study, we evaluated the effects of photodynamic treatment with red light and four phenothiazinium photosensitizers (ps): methylene blue (mb), toluidine blue o (tbo), new methylene blue n (nmbn) and the phenothiazinium derivative s ...201627623154
antibody-mediated pathogen resistance in plants.the methods described in this chapter were developed in order to produce transgenic plants expressing pathogen-specific single-chain variable fragment (scfv) antibodies fused to antifungal peptides (afps), conferring resistance against fungal pathogens. we describe the selection from a phage display library of avian scfv antibodies that recognize cell surface proteins on fungi from the genus fusarium, and the construction of scfv-afp fusion protein constructs followed by their transient expressi ...201626614296
identification of pathogenicity-related genes in fusarium oxysporum f. sp. cepae.pathogenic isolates of fusarium oxysporum, distinguished as formae speciales (f. spp.) on the basis of their host specificity, cause crown rots, root rots and vascular wilts on many important crops worldwide. fusarium oxysporum f. sp. cepae (foc) is particularly problematic to onion growers worldwide and is increasing in prevalence in the uk. we characterized 31 f. oxysporum isolates collected from uk onions using pathogenicity tests, sequencing of housekeeping genes and identification of effect ...201626609905
evolution of nine microsatellite loci in the fungus fusarium oxysporum.the evolution of nine microsatellites and one minisatellite was investigated in the fungus fusarium oxysporum and sister taxa fusarium redolens and fusarium verticillioides. compared to other organisms, fungi have been reported to contain fewer and less polymorphic microsatellites. mutational patterns over evolutionary time were studied for these ten loci by mapping changes in core repeat numbers onto a phylogeny based on the sequence of the conserved translation elongation factor 1-α gene. the ...201626661928
reducing environmental risk of excessively fertilized soils and improving cucumber growth by caragana microphylla-straw compost application in long-term continuous cropping systems.continuous cropping is a common agricultural practice in the word. in china, farmers often apply excessive fertilizers to fields in an attempt to maintain yields in continuous cropping systems. however, this practice often results in high nutrient concentrations in soils, nutrient pollution in leaching water and more crop disease. here, we investigated 8 different soils from continuously cropped cucumbers in northern china that grouped into those with extremely high nutrient levels (ehnl) and th ...201626657371
liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of fusarium oxysporum.fusarium oxysporum l11 is a non-pathogenic soil-borne fungal strain that yielded an extract that showed antifungal activity against phytopathogens. in this study, reversed-phase high-performance liquid chromatography (rp-hplc) coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry (api-qtof-ms) was applied for the comprehensive profiling of the metabolites from the extract. the employed sources were electrospray (esi), atmospheric pressure chemic ...201626655791
a conserved co-chaperone is required for virulence in fungal plant pathogens.the maize pathogenic fungus ustilago maydis experiences endoplasmic reticulum (er) stress during plant colonization and relies on the unfolded protein response (upr) to cope with this stress. we identified the u. maydis co-chaperone, designated dnj1, as part of this conserved cellular response to er stress. ∆dnj1 cells are sensitive to the er stressor tunicamycin and display a severe virulence defect in maize infection assays. a dnj1 mutant allele unable to stimulate the atpase activity of chape ...201626487566
fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics.molecular changes elicited by plants in response to fungal attack and how this affects plant-pathogen interaction, including susceptibility or resistance, remain elusive. we studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and fusarium oxysporum f. sp. ciceri (foc), using quantitative label-free proteomics and nmr-based metabolomics. results demonstrated differential expression of proteins and metabolites upon foc inoculations in the resist ...201626801007
isolation of n2 -fixing rhizobacteria from lolium perenne and evaluating their plant growth promoting traits.twenty one dinitrogen (n2 )-fixing bacteria were isolated from the rhizosphere of lolium perenne grown for more than 10 years without n-fertilization. the nearly complete sequence of the 16s rrna gene of each strain and pairwise alignments among globally aligned sequences of the 16s rrna genes clustered them into nine different groups. out of the 21 strains, 11 were members of genus bacillus, 3 belonged to each one of genera paenibacillus and pseudoxanthomonas, and the remaining 2 strains to eac ...201626781208
suicidal germination for parasitic weed control.parasitic weeds of the genera striga and orobanche spp. cause severe yield losses in agriculture, especially in developing countries and the mediterranean. seeds of these weeds germinate by a chemical signal exuded by the roots of host plants. the radicle thus produced attaches to the root of the host plant, which can then supply nutrients to the parasite. there is an urgent need to control these weeds to ensure better agricultural production. the naturally occurring chemical signals are strigol ...201626733056
oxysporizoline, an antibacterial polycyclic quinazoline alkaloid from the marine-mudflat-derived fungus fusarium oxysporum. 201626732255
the role of strigolactones during plant interactions with the pathogenic fungus fusarium oxysporum.strigolactones (sls) do not influence spore germination or hyphal growth of fusarium oxysporum. mutant studies revealed no role for sls but a role for ethylene signalling in defence against this pathogen in pea. strigolactones (sls) play important roles both inside the plant as a hormone and outside the plant as a rhizosphere signal in interactions with mycorrhizal fungi and parasitic weeds. what is less well understood is any potential role sls may play in interactions with disease causing micr ...201626725046
a hemocyanin-derived antimicrobial peptide from the penaeid shrimp adopts an alpha-helical structure that specifically permeabilizes fungal membranes.hemocyanins are respiratory proteins with multiple functions. in diverse crustaceans hemocyanins can release histidine-rich antimicrobial peptides in response to microbial challenge. in penaeid shrimp, strictly antifungal peptides are released from the c-terminus of hemocyanins.201626708991
isolation and identification of biocontrol agent streptomyces rimosus m527 against fusarium oxysporum f. sp. cucumerinum.actinomycetes have received considerable attention as biocontrol agents against fungal plant pathogens and as plant growth promoters. in this study, a total of 320 actinomycetes were isolated from various habitats in china. among which, 77 strains have been identified as antagonistic activities against fusarium oxysporum f. sp. cucumerinum which usually caused fusarium wilt of cucumber. of these, isolate actinomycete m527 not only displayed broad-spectrum antifungal activity but also showed the ...201627192632
a new biotype of fusarium oxysporum f. sp. lycopersici race 2 emerged by a transposon-driven mutation of avirulence gene avr1.emergence of races in fusarium oxysporum f. sp. lycopersici (fol) is caused by loss or mutation of at least one avirulence (avr) gene. the product of avr1 is a small protein (avr1) secreted by fol in tomato xylem sap during infection. this protein triggers fol race 1 specific resistance (i) in tomato, indicating that avr1 is an avr gene. deletion of avr1 in race 1 resulted in the emergence of race 2, and an additional mutation in avr2 generated race 3. previously, we reported a new biotype of ra ...201627190160
post-transcriptional silencing of the sge1 gene induced by a dsrna hairpin in fusarium oxysporum f. sp cubense, the causal agent of panama disease.fusarium oxysporum f. sp cubense (foc), the causal agent of panama disease, is responsible for economic losses in banana crops worldwide. the identification of genes that effectively act on pathogenicity and/or virulence may contribute to the development of different strategies for disease control and the production of resistant plants. the objective of the current study was to analyze the importance of sge1 gene expression in foc virulence through post-transcriptional silencing using a double-s ...201627173186
hyphal chemotropism in fungal pathogenicity.the ability to grow as filamentous hyphae defines the lifestyle of fungi. hyphae are exposed to a variety of chemical stimuli such as nutrients or signal molecules from mating partners and host organisms. how fungi sense and process this chemical information to steer hyphal growth is poorly understood. saccharomyces cerevisiae and neurospora crassa have served as genetic models for the identification of cellular components functioning in chemotropism. a recent study in the pathogen fusarium oxys ...201627150623
association of putative fungal effectors in fusarium oxysporum with wilt symptoms in soybean.fungi within the fusarium oxysporum species complex can cause root rot, seedling blight, and wilt of soybean. isolates recovered from soybean vary in aggressiveness and also the type of symptoms they produce. the aim of this study was to identify genetic markers to detect aggressive soybean wilt isolates. eighty isolates collected primarily from soybean were tested in the greenhouse for their ability to produce wilt symptoms using susceptible 'jack' soybean. the same 80 isolates were assessed fo ...201627146104
jasmonate signalling and defence responses in the model legume medicago truncatula-a focus on responses to fusarium wilt disease.jasmonate (ja)-mediated defences play important roles in host responses to pathogen attack, in particular to necrotrophic fungal pathogens that kill host cells in order to extract nutrients and live off the dead plant tissue. the root-infecting fungal pathogen fusarium oxysporum initiates a necrotrophic growth phase towards the later stages of its lifecycle and is responsible for devastating fusarium wilt disease on numerous legume crops worldwide. here we describe the use of the model legume me ...201627135231
h1-a, a compound isolated from fusarium oxysporum inhibits hepatitis c virus (hcv) ns3 serine protease.the present study was aimed to isolate the active compounds from the fermentation products of fusarium oxysporum, which had hepatitis c virus (hcv) ns3 protease inhibitory activity. a bioactive compound was isolated by reverse-phase silica-gel column chromatography, silica-gel column chromatography, semi-preparative reverse-phase high performance liquid chromatography (hplc), and then its molecular structure was elucidated based on the spectrosopic analysis. as a result, the compound (h1-a, 1) e ...201627114318
detection of plant growth enhancing features in psychrotolerant yeasts from patagonia (argentina).this study explores the biotechnological potential for plant production of twelve psychrotolerant yeasts strains from northwest-patagonia. these strains were isolated from different substrates associated with nothofagus sp. in native forests and vaccinium sp. in a commercial plantation. yeasts characterization was performed using in vitro assays to evaluate the production of auxin-like compounds and siderophores, ability to solubilize inorganic phosphate and to reduce common plant pathogen growt ...201627106520
regiospecific synthesis of prenylated flavonoids by a prenyltransferase cloned from fusarium oxysporum.due to their impressive pharmaceutical activities and safety, prenylated flavonoids have a high potent to be applied as medicines and nutraceuticals. biocatalysis is an effective technique to synthesize prenylated flavonoids. the major concern of this technique is that the microbe-derived prenyltransferases usually have poor regiospecificity and generate multiple prenylated products. in this work, a highly regiospecific prenyltransferase (fopt1) was found from fusarium oxysporum. it could recogn ...201627098599
new fusaric acid derivatives from the endophytic fungus fusarium oxysporum and their phytotoxicity to barley leaves.chemical investigation of the endophytic fungus fusarium oxysporum isolated from fruits of drepanocarpus lunatus afforded eight new fusaric acid derivatives, fusaricates a-g, 1-7, and 10-hydroxy-11-chlorofusaric acid, 8, along with four known compounds. their structures were elucidated by one- and two-dimensional nmr as well as ms data and by comparison with the literature. the absolute configurations of fusaricates c-e, 3-5, were determined using chiral gc-ms. fusaricates a-g, 1-7, represent th ...201627050289
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