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effects of management practices on nematode and fungi populations and okra yield.okra was grown in field plots of tifton loamy sand naturally infested with the nematodes meloidogyne incognita and criconemoides ornalus and the pathogenic fungi fusarium oxysporum, f. solani, f. roseum, and pythium spp. plots were treated with various soil pesticides and left exposed or covered with biodegradable paper film mulch under trickle irrigation. soil was assayed for nematodes and fungi, and plant roots were examined for root-rot and insect damage. fewer nematodes and fungi generally w ...197719305580
the occurrence of 12, 13-epoxytrichothecenes in seeds of safflower infected with fusarium oxysporum f. sp. carthami.from the seeds of safflower (carthamus tinctorius linn.), infected with fusarium oxysporum f. sp. carthami, 3 toxic compounds have been isolated in quantities sufficient to cause mycotoxicosis on prolonged ingestion. 2 of these have been identified as diacetoxyscirpenol and t-2 toxin, while the third one has also been partially characterized as a 12, 13-epoxytrichothecene. additionally, the incidence of secondary fusarial infection of healthy seeds due to contamination with the infected ones has ...1977862770
effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph.fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ...197718085
studies on sterol-ester hydrolase from fusarium oxysporum. i. partial purification and properties.1. a search for a long chain fatty acyl sterol-ester hydrolase in microorganisms led to the isolation from soil of five strains belonging to fusarium sp. which produced strong activity in the culture medium. 2. the cholesterol esterase from fusarium oxysporum igh-2 was purified about 270-fold by means of cacl2 precipitation and sephadex g-75 column chromatography. 3. the cholesterol esterase was activated by adekatol and triton x-100. it was inhibited by lecithin and lysolecithin, and completely ...197719426
utilization of kerosene by a hydrocarbon-assimilating strain of fusarium oxisporum.fusarium oxysporum ifo 6384 was grown in three different media, one containing glucose and two containing kerosene as carbon source. one of the kerosene media contained also tween 60. the harvested dry mats were weighed at various stages of growth. substitution of glucose with kerosene led to a sharp drop in the production of fungal mycelia. analysis of the mat samples representing maximum yields indicated that the mycelia grown in the kerosene media were richer in free amino acid and poorer in ...1977602572
effect of three nematicides on the growth of some phytopathogenic bacteria and fungi.the effect of three nematicides, aldicarb, fensulfothion, and phenamiphos at four concentrations (1, 5, 25, and 125 ppm) was tested on the growth of five bacteria, agrobacterium tumefaciens, corynebacterium fascians, erwinia carotovora, pseudomonas solanacearum, and streptomyces scabies and four fungi, fusarium oxysporum f. sp. vasinfectum, fusarium solani, rhizoctonia solani, and sclerotium bataticola. of the bacteria, p. solanacearum was most affected by the chemicals at all concentrations, wh ...1977143857
regulation of pyruvate kinases from fusarium oxysporum.two types of pyruvate kinases were found in fusarium oxysporum. one type (inducible) was present mainly during the early stages of growth on glucose or sucrose and displayed michaelis-menten kinetics with respect to phosphoenolpyruvate and adenosine diphosphate. the major type (constitutive) was present under all conditions of growth and displayed in the absence of potassium ions, a sigmoidal substrate saturation curve when phosphoenolpyruvate was used as the variable substrate. in the presence ...197721735
effect of different concentrations of dl-isoleucine, dl-valine, and dl-alanine on growth and sporulation in fusarium oxysporum f. udum (butl.) sn. et h.d1-alanine and dl-valine, when added as an extra nitrogen for fortifying the already present inorganic nitrogen source, actually acted as growth retardant for f. oxysporum f. udum (butl.) sn. et h. sporulation of microconidia was indifferently affected by these two amino acids. di-valine stimulated microconidial formation in young cultures only. in both young and old cultures the lowest concentration of dl-valine depressed macronidial sporulation. in old cultures the lowest concentration of vali ...1977610233
toxicological approaches to the metabolites of fusaria. xi. trichothecenes and zearalenone from fusarium species isolated from river sediments.fifty isolates of fusarium species from river sediments were examined on their producibility of mycotoxins such as trichothecenes and zearalenone. the most frequent fungal isolate was fusarium oxysporum, followed by f. solani and f. lateritium. when cultured on czapek-dox medium supplemented with peptone, more than half of these strains were suspected to produce trichothecene mycotoxins through toxicity to mice and reticulocyte assay method. one of toxic isolates, f. lateritium 5036, was proved ...1977916296
circular dichroism of fusarium lipoxygenase from fusarium oxysporum. 197833673
purification and properties of nitroalkane oxidase from fusarium oxysporum.a nitroalkane-oxidizing enzyme, which was inducibly formed by addition of nitroethane to the medium was purified to homogeneity from an extract of fusarium oxysporum (ifo 5942) with an overall yield of about 20%. the enzyme catalyzed the oxidative denitrification of 1-nitropropane as follows: ch2(no2)ch2ch3 + o2 + h2o leads to ohcch2ch3 + hno2 + h2o2. in addition to 1-nitropropane, 3-nitro-2-pentanol, 2-nitropropane, and nitrocyclohexane are good substrates; the enzyme is designated "nitroalkane ...197822538
crystallization and positional specificity of hydroperoxidation of fusarium lipoxygenase.a lipoxygenase obtained from the fungus fusarium oxysporum was purified and crystallized. using the purified enzyme, the positional specificity of linoleate peroxidation was studied. linoleate hydroperoxides were converted into the corresponding trimethylsilyl derivative by reduction, catalytic hydrogenation and treatment with hexamethyldisilazane/trimethylchlorosilane/pyridine and then analyzed by combined gas-liquid chromatography-mass spectrometry. fusarium lipoxygenase was found to produce 9 ...1978100142
hydroxymethylglutaryl-coenzyme a reductase. purification and properties of the enzyme from fusarium oxysporum.the hydroxymethylglutaryl-coenzyme a reductase (mevalonate:nadp+ oxidoreductase, ec 1.1.1.34) system in fusarium oxysporum, a soil inhabiting plant pathogen, has been examined. two forms of the enzyme catalyzing the conversion of hydroxymethylglutaryl-coenzyme a were obtained in the supernatant after precipitation at 75% (nh4)2so4 saturation of the soluble culture extract which was previously separated from cell wall, mitochondria and microsomes. the two forms of the enzyme were separated electr ...197826407
effect of c.c.c. [2-(chloroethyl)] trimethyl ammonium chloride on growth and sporulation in fusarium oxysporum f. udum (butl.) sn. et h.in fusarium oxysporum f. udum sporulation of macroconidia was completely inhibited due to the influence of c.c.c. [2-(chloroethyl)] trimethyl ammonium chloride). microconidial proliferation, on the other hand, was adversely affected and with increase in concentration of this compound a parallel and proportional decrease in the amount of microconidia was found. size of microconidia, too, showed a concomitant decline. although the size of the chlamydospores was also reduced, a tremendous increase ...1978664938
effect of different cultural conditions on the chemical composition of lipids of fusarium oxysporum. 1978723227
lipid metabolism in fusarium oxysporum. 1978723228
the influence of water-logging stage upon the paddy soil mycoflora in the ukraine.the soil fungal flora of paddy soils from the southern districts of the ukraine was investigated. from 287 soil samples 170 species were isolated. they belonged to the following genera: oomycetes 1.2%, zygomycetes 8.8%, ascomycetes 5.8%, deuteromycetes 84.2%. certain fungi were found to be dominant forms, among them mortierella alpina peyron., penicillium funiculosum thom, p. rugulosum thom, p. chrysogenum thom, acremonium strictum w. gams, acr. breve w. gams, alternaria alternata (fr.) riessler ...1978726714
growth temperature-dependent stearoyl coenzyme a desaturase activity of fusarium oxysporum microsomes.the characteristics of the microsomal stearoyl coa desaturase (ec 1.14.99.5) of vegetative fusarium oxysporum cells grown at different temperatures were studied. the enzyme had an unusual preference for nadph (km = 38 micrometers) over nadh (km = 89 micrometers) as electron donor, and a relatively high optimum ph of 8.3. enzyme activity was highest in microsomes from cells grown at 37 degrees c and lowest in cells grown at 15 degrees c. this result correlated well with the observed changes in ol ...197835271
influence of soil fumigation on the fusarium-root-knot nematode disease complex of cotton in california.for control of the root-knot nematode, meloidogyne incognita, and the pathogenic wilt fungus, fusarium oxysporum, on cotton, soil fumigants were applied in the field at conventional and higher rates. conventional rates suppressed fusarium wilt but higher rates gave quicker early growth, better stands, less stand loss over the season, a lower percentage of plants infected with wilt, fewer plants with vascular discoloration, and fewer nematodes. the best treatment about doubled the yields of untre ...197819305846
interaction of population levels of fusarium oxysporum f. sp. vasinfectum and meloidogyne incognita on cotton.in autoclaved greenhouse soil without fusarium oxysporum f. sp. vasinfectum, meloidogyne incognita did not cause leaf or vascular discoloration of 59-day-old cotton plants. plants had root galls with as few as 50 meloidogyne larvae per plant. root galling was directly proportional to the initial nematode population level. fusarium wilt symptoms occurred without nematodes with 77,000 fungus propagules or more per gram of soil. as few as 50 meloidogyne larvae accompanying 650 fungus propagules cau ...197919305546
populations of the spinach wilt pathogen, fusarium oxysporum f. sp. spinaciae, in the root tissues, rhizosphere, and soil in the field.populations of fusarium oxysporum f. sp. spinaciae in root tissues and rhizosphere soil of diseased spinach plants were higher than in the root tissues and rhizosphere soil of healthy plants. populations in soil rhizosphere were higher than in nonrhizosphere soil. the fungus populations were very low in the root tissues of the nonsusceptible strawberry, broccoli, chinese cabbage, and mustard grown in the infested field. the populations were low at the beginning of the season, increased, and rema ...1979436018
production of 3-acetoxyscirpene-4,15-diol from anguidine (4,15-diacetoxyscirpene-3-ol) by fusarium oxysporum f.sp. vasinfectum.growing cells of fusarium oxysporum f.sp. vasinfectum (atcc 7808) formed 3-acetoxyscirpene-4,15-diol from anguidine (4,15-diacetoxyscirpene-3-ol) by way of the intermediates triacetoxyscirpene, 3,4-diacetoxyscirpene-15-ol and 3,15-diacetoxyscirpene-4-ol. the new 3-acetoxy analog was found to be less active than anguidine and the other monoacetoxy derivatives when tested against a series of fungal strains and against hela cells in vitro.1979453837
the effect of temperature and age of strains of fusarium oxysporum on its enzymatic activity.the activities of pectin-methyl-esterase, polygalacturonase, cellulase, amylase, saccharase, and protease of strains of fusarium oxysporum (schlecht.) f.sp. pisi (linford) were studied. the selected strains showed different symptoms and different degrees of pathogenicity on the host plant. the measurements were performed during the growth of strains at constant temperatures and, in another experiment, on the day at different temperatures at which the individual strains were grown. activities wer ...1979473966
different manifestations of the pathogenity of some strains of fusarium oxysporum f. sp. pisi.different cultivation and morphology characteristics were found in 10 monospore isolates of fusarium oxysporum f. sp. pisi, obtained from yellowing and wilting plants of pea (pisum sativum l.). the isolates of the fungus were obtained from distant geographical regions of czechoslovakia and from various cultivars and hybrids of pea. after inoculation of roots, followed by constant conditions of incubation of the meteor and jupiter cultivars having their origin at the plant-breeding station at luz ...1979473967
the fine structure of mature and germinating chlamydospores of fusarium oxysporum.a number of features not described previously has been revealed in electron-microscope studies of mature chlamydospores of fusarium oxysporum. on the maturation of one isolate, many spores formed a thick matrix-like layer containing electron-dense particles on the exterior surface of the spore wall. in thin sections of mature chlamydospores of the same isolate, cisternae of endoplasmic reticulum surrounding, and in close apposition to, the limiting boundary of the lipid bodies were revealed. the ...1979476555
studies on the extracellular cellulase (cx) from fusarium oxysporum. 1979535933
isolation of metarrhizium anisopliae, beauveria tenella and fusarium oxysporum (deuteromycetes) and their pathogenicity to culex fatigans and anopheles stephensi. 1979535971
vitamin requirements of fusarium oxysporum f. sp. vasinfectum.the effect of eight water-soluble vitamins on germination, germ-tube extension, growth, and sporulation of fusarium oxysporum schl. f.sp. vasinfectum (atk.) snyder and hansen, was studied. each vitamin was utilized in eight different concentrations. the fungus responded favourably to all of the utilized vitamins in almost all the concentrations where germination, growth, and sporulation were substantially greater than the controls. among the vitamins used, the fungus appeared to be highly sensit ...1979543346
changes in metabolic activities of fusarium oxysporum f. fabae and rhizoctonia solani in response to dithan a-40 fungicide.the effect of different concentrations of dithan a-40 fungicide on the metabolic activities of the wilt fungus fusarium oxysporum f. fabae and the root rot agent rhizoctonia solani was studied. all toxicant concentrations reduced energy generation, total phosphorus and nitrogen content of both fungi. in addition, the toxicant caused a shift in free amino acids pool. as a result of these changes, the mycelium dry weight of both fungi was greatly reduced. r. solani was more sensitive to the toxic ...1979543918
effect of leaf extract of garlic on fusarium oxysporum f. sp. ciceri, sclerotinia sclerotiorum and on gram seeds. 1979574190
fusarium oxysporum endophthalmitis.a 27-year-old man sustained a thorn injury to his left eye and a culture-proven fusarium oxysporum endophthalmitis developed. this was successfully treated with a vitrectomy, intravitreal and intravenous amphotericin b, and oral flucytosine. the patient maintains 20/20 vision at this time in his left eye. we recommend combined therapy for this filamentous fungal infection.1979310293
comparative studies with regard to the influence of carbon and nitrogen ratio on sporulation in fusarium oxysporum and fusarium moniliforme v. subglutinans.carbon/nitrogen ratio as a factor for sporulation, expressed in terms of magnitude of population variation of macroconidia and microconidia in the cultures of eusarium oxysporum schlecht ex. fr., fusarium moniliforme v. subglutinans wr. and rg., and of chlamydospores (only in fusarium oxysporum) was investigated. it has been found that the amount of carbon source shapes the course of macro- and micro. conidial production in a linear fashion, being enhanced parallel to the increase in its amount- ...1979543920
opportunistic fungal infection by fusarium oxysporum in a renal transplant patient.a white female with chronic glomerular nephritis received a renal transplant in 1971. after 1 year, livido reticularis (lr) developed and in 1976 erythematous, painful nodules formed on the lr and ulcerated. the patient also suffered diffuse calcification of the major blood vessels and small arterioles of the extremities with progressive necrosis and gangrene of the fingers. hyperparathyroidism was evident. the necrotic ulcers yielded candida albicans and fusarium oxysporum; both organisms were ...1979394364
fusarium oxysporum endophthalmitis. 1979313780
developmental regulation of invertase isozymes in fusarium oxysporum.fusarium invertase has two isozymes; one is developmentally regulated, whereas the other exists throughout the developmental stages of fusarium.1979457615
lipid involvement in oleoyl coa desaturase activity of fusarium oxysporum microsomes.desaturation of oleoyl coa by the microsomal fraction of fusarium oxysporum hyphal cells required o2, nadph, mgcl2, and the addition of either bovine serum albumin or the 105 000g supernatant fraction. in the absence of reduced nucleotide, [14c]oleoyl coa was rapidly incorporated into phospholipid and triacylglycerol and hydrolyzed to free fatty acids. after addition of nadph, oleate was desaturated at the normal rate. analysis of the distribution of [14c]oleate and [14c]linoleate between differ ...19806104537
effect of volatile and gaseous metabolites of germinating pea seeds on micromycetes.differences in the effect of volatile and gaseous metabolites of germinating pea seeds on the germination of spores of mucor racemosus and macroconidia of fusarium oxysporum are described. germination of spores of m. racemosus was inhibited by seed metabolites whereas germination of macroconidia of f. oxysporum was stimulated during the first two days and inhibition occurred only after further two days of germination of the seeds. a pronounced inhibition of germination of spores of both micromyc ...19806769770
volatile and gaseous metabolites released by germinating seeds of lentil and maize cultivars with different susceptibilities to fusariosis and smut.the effect of volatile and gaseous metabolites released by germinating seeds of lentil cultivars more and less susceptible to fusariosis on the germination of spores of mucor racemosus, trichoderma viride, verticillium dahliae and botrytis cinerea was found to depend rather on the fungal genus than on the lentil cultivar. however, spores of fusarium oxysporum reacted more sensitively during germination to the presence of exudates of both cultivars, when the more susceptible lentil displayed a st ...19807189738
ethylene production by ectomycorrhizal fungi, fusarium oxysporum f. sp. pini, and by aseptically synthesized ectomycorrhizae and fusarium-infected douglas-fir roots.the ectomycorrhizal fungi cenococcum geophilum, hebeloma crustuliniforme, and laccaria laccata produced ethylene in vitro in modified melin-norkrans liquid medium only if amended with 2.5-10mm methionine; pisolithus tinctorius failed to produce ethylene unless the cultures were renewed with fresh methionine-amended medium before ethylene assay. an additional 19 ectomycorrhizal fungi, plus five isolates of fusarium oxysporum f. sp. pini, all produced ethylene in renewed and (or) nonrenewed media. ...19807214223
the effect of the herbicide atrazine on rhizosphere microflora of broad bean plants, infested with fusarium oxysporum f. fabae and rhizoctonia solani.the effect of the herbicide atrazine on fusarium oxysporum f. fabae and rhizoctonia solani, the causative agents of wilt and rot, respectively, as well as rhizosphere microflora of broad bean plants was studied. the herbicide was applied at intervals of 30, 20, 10, and 0 days before sowing. atrazine application reduced both densities and per cent of infection of these two pathogenic fungi, r. solani, however, was sensitive to atrazine itself, while f. oxysporum was affected by atrazine by-produc ...19807376757
a case of nondermatophytic toe onychomycosis caused by fusarium oxysporum.the case history of a patient with nondermatophytic onychomycosis of the great toenail due to fusarium oxysporum is presented. the occurrence of fusarium onychomycosis, although not rare, is infrequent. this fungus may not be recovered from clinical material if the specimen is cultured only on media containing cycloheximide.19806445716
sterol ester hydrolase in fusarium oxysporum.two electrophoretically different forms of sterol ester hydrolase (ec 3.1.1.13) were obtained from the cytoplasmic extract of the mycelia of fusarium oxysporum. the entities, estimated at 60,000 (i) and 15,000 (ii) molecular weights, were obtained in sephadex g100 column chromatography of the ammonium sulfate precipitate from the cytoplasmic extract. a third form iii, 75,000 mw, was obtained from the culture filtrate. the activity of the enzyme was increased by triton x-100 and was not inhibited ...19817242270
[date palm and fusariosis. viii.--parasitism of "fusarium oxysporum" f. sp. "albedinis" by an actinomycete (author's transl)].fortuitous growth of an actinomycete on fusarium oxysporum f. sp. albedinis culture has shown a host-parasite process. as a response to the actinomycete, the fungus produces thallospores with various forms which can germinate faster than the non-parasited f. o. albedinis microconidies. however, the strains obtained from thallospores showed as sensible as the mother strain towards actinomycete action.19817294610
methionine transport by mycelia of fusarium oxysporum f. sp. lycopersici.mycelia of fusarium oxysporum f. sp. lycopersici accumulated l-methionine by an energy-dependent process, and the energy required for uptake may be derived from either respiration or glycolysis. the ph optimum for transport was 4 and the temperature optimum was 35 degrees c. the apparent km for methionine uptake was 2.5-3.3 microm and the vmax 0.24-0.30 mmol . min-1 . mg dry weight-1. s-adenosylhomocysteine (ado-hcys) was the major metabolic product of methionine although s-adenosylmethionine (a ...19817296408
enzymatic and microbial preparation of d-xylulose from d-xylose.a high-d-xylulose mixture (d-xylose-d-xylulose = 33:67) was prepared from the cold ethanol extract of preisomerized d-xylose solution (d-xylose-d-xylulose = 77:23). fusarium oxysporum f. sp. lini and aspergillus niger were demonstrated to preferentially utilize d-xylose in the mixture of d-xylose and d-xylulose. chromatographically pure d-xylulose was thus obtained in 90% yield. a high-d-xylulose mixture was also incubated with rhodotorula toruloides, klebsiella pneumoniae, candida utilis, or mu ...198116345816
influence of meloidogyne incognita on fusarium wilt of tomato at or below the minimum temperature for wilt development.'bonny best' tomato plants were grown at 16, 21, or 24 c for 28 d in soil infested with either of two isolates of fusarium oxysporum f. sp. lycopersici race 1 and meloidogyne incognita. significant levels of fusarium wilt occurred at all temperatures including 16 c, which has not been reported previously. one fusarium isolate resulted in the highest levels of disease incidence at 21 and 24 c in the presence of root-knot nematodes, and at 24 c when the nematodes were not present. at 16 c there wa ...198119300723
influence of nonhosts, crucifers, and fungal parasites on field populations of heterodera schachtii.heterodera schaehtii egg number decline under nonhosts was surveyed for 3-4 years at soil depths of 0-30 cm and 30-60 cm in three fields in the imperial valley, california. in the two fields continously cropped to alfalfa, annual decline rates were 49 and 63%, respectively, and did not differ (p = 0.05) between depths. in the third field, cropped to annual nonhosts and fallowed, decline rates of 56 and 80% at 0-30-cm and 30-60-cm depths, respectively, were significantly different (p = 0.05). egg ...198119300739
suppression of alfalfa growth by concommitant populations of pratylenchus penetrans and two fusarium species.growth of alfalfa (medicago sativa cv. vernal) seedlings was compared after inoculation with combinations of either pratylenchus penetrans and fusarium soloni or p. penetrans and f. oxysporum f. sp. medicaginis. a synergistic disease interaction occurred in alfalfa when f. oxysporum and p. penetrans were added simultaneously to the soil. alfalfa growth was suppressed at all inoculum levels of p. penetrans and f. oxysporum, but not with f. solani. seedlings inoculated with the nematode alone gave ...198219295722
enniatin synthetase, a novel type of multifunctional enzyme catalyzing depsipeptide synthesis in fusarium oxysporum.enniatin synthetase, a multifunctional enzyme catalyzing depsipeptide formation in fusarium oxysporum was purified to 98% homogeneity as judged by analytical disc gel electrophoresis. the enzyme consists of a single polypeptide chain of a molecular weight of about 250 000. similar to a number of peptide synthetases and to fatty acid synthetase the enzyme contains 4'-phosphopantetheine as a prosthetic group. studies on substrate specificity revealed that the enzyme is capable of synthesizing enni ...19826895851
8006-i, an antibiotic from amblyosporium spongiosum (pers.) hughes sensu pirozynski. ii. biological properties.8006-i is an antibacterial antibiotic with a rather broad spectrum of activity. the minimum inhibitory concentrations for the most sensitive bacteria are in the range of one to ten micrograms/ml. yeasts are not affected by concentrations up to 100 micrograms/ml. some filamentous fungi like fusarium oxysporum and mucor miehei are inhibited at 100 micrograms/ml. in ehrlich carcinoma ascitic cells the incorporation of uridine and leucine and to a lesser extent that of thymidine is reduced. in isola ...19826178718
development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues.we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ...19836195179
3'-hydroxylation of 4'-methoxyisoflavones by fusarium oxysporum f. lycopersici.3'-hydroxylation of isoflavones by fusarium oxysporum f. lycopersici mainly proceeds with 4'-methoxy-7-hydroxyderivatives; this reaction is used for quantitative conversion of 14c-labelled isoflavones.19836649792
purification and properties of a cytochrome p-450 of a fungus, fusarium oxysporum.a cytochrome p-450 was isolated from a fungus, fusarium oxysporum, which grew on a medium containing soybean oil as a sole carbon source. it was found as a heme protein that possess lipoxygenase activity, and seemed to exist in the soluble fraction of cell-free extracts. the cytochrome revealed multiplicity and could be separated into a least 3 fractions (a, b, and c). two of them, termed fusarium p-450a and -b, were highly purified. the complex of the ferrous fusarium p-450 with carbon monoxide ...19836654854
microbial degradation of trifluralin by aspergillus carneus, fusarium oxysporum and trichoderma viride.3h-trifluralin was synthesized by condensation of 3h-4-chloro-3,5-dinitro-alpha, alpha, alpha-trifluorotoluene with di-n-propylamine. after incubation of trifluralin with aspergillus carneus, fusarium oxysporum and trichoderma viride for 10 days, a small percentage (less than 10%) of unchanged herbicide was recovered in the extractable fraction. this indicates a fairly rapid degradation of the herbicide by the fungal species. other than trifluralin, the culture medium contained at least five lab ...19836682873
syntheses of some 4,4'-biphenylenedihydrazone-3-(3-methyl)-2-pyrazolin-4,5-dione derivatives and their antimicrobial potentialities.a new series of bispyrazolones were obtained upon treating the bishydrazone derivative 1 with dimethylsulphate, acetic anhydride, benzenesulphonyl chloride, piperidine, morpholine, n-methylaniline and formaldehyde respectively. a mannich base was prepared from the interaction of 4-phenylhydrazone-3-methyl-2-pyrazolin-5-one and n-butylamine. the condensation of an n-hydroxy methyl derivative with ethyl acetoacetate and diethyl malonate led to the formation of the corresponding n-methyl derivative ...19836867077
iron-chelating compounds produced by soil pseudomonads: correlation with fungal growth inhibition.strains of pseudomonas putida, pseudomonas sp., and pseudomonas aeruginosa were examined for their ability to grow in the presence of the iron chelator, ethylenediamine-di-(o-hydroxyphenylacetic acid). in vitro fungal inhibition assays showed that the isolates varied in their ability to inhibit the growth of representative fungal plant pathogens. fungal inhibition in vitro was superior to that of previously reported pseudomonas sp. studies with fusarium oxysporum forma sp. lycopersici and a susc ...198316346334
conversion of helminthosporium sacchari toxin to toxoids by beta-galactofuranosidase from helminthosporium.helminthosporium sacchari produces a host-selective toxin and structurally related nontoxic compounds, here referred to as ;toxoids.' toxin and the three toxoids were each isolated to a high level of purity and were hydrolyzed under acidic conditions. the released galactose was measured by a galactose oxidase/peroxidase assay. toxin was found to contain four units of galactose per molecule, as previously reported. toxoids i, ii, and iii contained one, two, and three units of galactose, respectiv ...198316663037
ultrastructural changes caused by fusarium oxysporum f. sp. lycopersici in meloidogyne javanica induced giant cells in fusarium resistant and susceptible tomato cultivars.tomato (lycopersicon esculentum mill.) seedlings, susceptible (cv. pearson a-i improved) and resistant (cv. pearson improved) to race 1 fusarium oxysporum f. sp. lycopersici (sacc.) snyd &hans., were inoculated with meloidogyne javanica (trueb) chitwood second-stage juveniles and 3 weeks later with race 1 f. oxysporum f. sp. lycopersici spores. one week after fungal inoculation, no fungus was visible in root tissue of the tomato cultivars and the giant cells were normal. two weeks after fungal i ...198319295778
mineralization of cellulose in the presence of chitin and assemblages of microflora and fauna in soil.nitrogen content is an important factor controlling decomposition of resistant substrates. we examined the decomposition of purified cellulose in the presence of a structurally similar, resistant organic n compound, chitin. carbon-14-labelled cellulose was added to sterile ari-dried sandy loam soil in flasks, half of which were also amended with purified chitin. grassland soil organisms-a fungus (fusarium oxysporum) or a bacterium (flavobacterium sp.) with or without their respective nematode gr ...198328310541
hydrolysis of xylan and fermentation of xylose to ethanol.the focus in the development of pulping processes has usually been exclusively on cellulose. however, hemicellulose could serve as a valuable source of hexose and pentose sugars. consequently, it should not be destroyed in a process designed for very high cellulose fibre yields. novel procedures developed for production of ethanol by the fermentation of pentoses as well as hexoses provide new possibilities of hemicellulose utilization. many fungi produce extracellular hemicellulases. in the pres ...198414545698
effect of a new fungicide (pndp) on the minor nucleotide content of fusarium oxysporum.the minor nucleotide constituents of rrna from the plant pathogenic fungus fusarium oxysporum were investigated. when the fungus was grown for 8 days in aerated liquid culture the following minor nucleotides were identified from 18s and 25s rrna: m6a, m2a , m2(6)a, m1g, m2g , m2(2)g, m3c , cm, m5c, m3u , um, cm5u , rt and psi. pndp (+/- threo-1-phenyl-2-nitro-1,3 diacetoxy-propane), a new fungicide of unknown biochemical mode of action, caused a great decrease in the minor nucleotide content of ...19846741453
mitochondrial dna in fusarium oxysporum is a 46.5 kilobase pair circular molecule.purified mitochondria were obtained from the phytopathogenic fungus fusarium oxysporum f. sp. lycopersici by mechanical disruption of protoplasts, followed by differential and density gradient centrifugation. dna, extracted from the mitochondria, was shown by electron microscopy and restriction endonuclease analysis to be a 46.5 kilobase pair circular molecule.19846098631
uptake and retention of 14c-aroclor 1254 in the amphipod, gammarus tigrinus, fed contaminated fungus, fusarium oxysporum. 19853919657
mycotoxins producing fungi and mycoflora of air-dust from taif, saudi arabia.using the dilution plate method, 70 species and 31 genera were collected from 20 dust samples on glucose (28 genera and 64 species) and cellulose czapek's agar (22 genera and 46 species) at 28 degrees c. the most common fungi were aspergillus niger, a. flavus, a. flavus var. columnaris, phoma glomerata, fusarium oxysporum, penicillium chrysogenum and mucor racemosus; and a. nidulans, phoma humicola, drechslera spicifera and stachybotrys chartarum on the two media, respectively. toxicity test sho ...19853935928
subterminal hydroxylation of fatty acids by a cytochrome p-450-dependent enzyme system from a fungus, fusarium oxysporum.the cell-free extract of a cytochrome p-450-producing fungus, fusarium oxysporum, was found to catalyze the hydroxylation of fatty acids. three product isomers were formed from a single fatty acid. the products from lauric acid were identified by mass-spectrometry as 9-, 10-, and 11-hydroxydodecanoic acids, and those from palmitic acid as 13-, 14-, and 15-hydroxyhexadecanoic acids. the ratio of the isomers formed was 50 : 36 : 14 in the case of laurate hydroxylation, and 37 : 47 : 16 in the case ...19854019433
synthesis and spectroscopic and fungicidal characterization of hydroxamic acids and their metal chelates.hydroxamic acid chelates of the type ml2, ml2', and ml2" where m = cu(ii), ni(ii) or co(ii) and l = n,2'-diphenylacetohydroxamic acid (n,2'-dpaha), l' = 2,2'-diphenylacetohydroxamic acid (2,2'-dpaha), and l" = 2-phenylacetohydroxamic acid (2-paha) have been isolated and characterized on the basis of elemental analysis and infrared and magnetic data. these metal chelates were screened for their fungicidal activity. the testing against fungi has been carried out by slide germination technique agai ...19854045444
chitinase-gold complex used to localize chitin ultrastructurally in tomato root cells infected by fusarium oxysporum f. sp. radicis-lycopersici, compared with a chitin specific gold-conjugated lectin.a cytochemical technique for the ultrastructural localization of chitin in tomato root cells infected by fusarium oxysporum f. sp. radicis-lycopersici is reported. chitinase was complexed to colloidal gold and thin sections were incubated with the enzyme-gold complex. this technique yielded a more uniform distribution of gold particles over the fungus wall, compared to that obtained with the lectin-gold technique. both techniques revealed no labelling of the fungus cytoplasm, except for organell ...19852411684
nematode control related to fusarium wilt in soybean and root rot and zinc deficiency in corn.nematode and disease problems of irrigated, double-cropped soybean and corn, and zinc deficiency of corn were investigated. ethylene dibromide, phenamiphos, and aldicarb were equally effective for controlling nematodes and increasing yields of corn planted minimum-till and soybean planted in a moldboard plow prepared seedbed. the residual effects on yields of nematicides applied to the preceeding crop occurred during 3 years for soybean and 1 year for corn. fusarium wilt symptoms of soybean that ...198519294099
correlations between in vivo resistance to fusarium and in vitro response to fungal elicitors and toxic substances in carnation.with the aim of ascertaining the existance of a correlation between in vivo resistance to fusarium oxysporum f. sp. dianthi and in vitro response to fungal elicitors and toxic substances, phenylalanine ammonialyase and phytoalexin accumulation, on one hand, and resistance to culture filtrate, on the other, were assayed in "in vitro" cultures of three susceptible and four resistant dianthus caryophyllus cultivars. cultivars showing varying degrees of resistance in vivo either tolerated higher cul ...198524254112
a single dominant gene for fusarium wilt resistance in protoplast-derived tomato plants.tomato plants resistant to the fungal pathogen, fusarium oxysporum f. sp. lycopersici, race 2, were obtained using in vitro selection against fusaric acid, a non-specific toxin, as well as non-challenged cells. protoplasts were isolated from cotyledonary tissue of tomato cv. 'uc-82', which is susceptible to fusarium race 2. protoplasts were challenged with the toxin, and the resistant calli were further subjected to the toxin. plants regenerated from toxin-resistant calli were screened for resis ...198624240846
mycoflora and mycotoxin-producing fungi of air-dust particles from egypt.using the dilution-plate method, 27 genera and 64 species were collected from 20 air-dust samples on glucose - (24 genera and 57 species) and cellulose - (21 genera and 45 species) czapek's agar at 28 degrees c. there are basic similarities between the mycoflora of air-dust on the two media and the most prevalent species were aspergillus niger, a. flavus, a. ochraceus, a. terreus, a. versicolor, penicillium chrysogenum, p. funiculosum, alternaria alternata, cladosporium herbarum, fusarium oxyspo ...19862938009
mycological survey of korean cereals and production of mycotoxins by fusarium isolates.the fungal species isolated from korean cereals (barley, polished barley, wheat, rye, and malt) were alternaria spp., aspergillus spp., chaetomium spp., drechslera spp., epicoccum sp., fusarium spp., and penicillium spp., etc. the number of fusarium strains isolated was 36, and their ability to produce fusarium mycotoxins on rice was tested. nivalenol (niv) was produced by fusarium graminearum (7 of 9 isolates), fusarium oxysporum (3 of 10 isolates), and fusarium spp. (7 of 15 isolates). of 15 i ...19862947538
extensive subcutaneous hyphomycosis caused by fusarium oxysporum.subcutaneous granulomatous lesions by hyaline moulds are rare. this report describes a case of hyalohyphomycosis by fusarium oxysporum. the patient was a 16-year-old sri lankan girl who had had extensive lesions in both arms almost from infancy. the fungus was demonstrated in koh mounts and in histopathological sections. it was isolated repeatedly from her lesions over a period of 10 months. combined therapy with ketoconazole and 5-fluorocytosine resulted in marked improvement. an inherent immun ...19863723288
primary intranasal fusarium infection. potential for confusion with rhinocerebral zygomycosis.fusarium species are saprophytic fungi that may colonize human skin and nails and may rarely cause invasive infections in traumatized tissue and in debilitated and immunocompromised patients. we report herein a case of invasive intranasal fusarium oxysporum infection in a diabetic patient. this unusual presentation potentially can be confused with early rhinocerebral zygomycosis clinically and histologically. distinguishing morphologic features and the possible role of diabetes in promoting this ...19863755330
parasitic invasion of fusarium oxysporum in an arterial ulcer in an otherwise healthy patient. 19863762587
linear plasmidlike dna in the plant pathogenic fungus fusarium oxysporum f. sp. conglutinans.double-stranded, 1.9-kilobase-pair (kbp) dna molecules were found in 18 strains representing three pathogenic races of fusarium oxysporum f. sp. conglutinans. the dna element (pfoxc1) from a race 1 strain and the dna element (pfoxc2) from a race 2 strain were shown by restriction endonuclease mapping to be linear. pfoxc2 was found in mitochondrial preparations and appears to have blocked 5' termini, as it was sensitive to 3'----5' exonuclease iii but insensitive to 5'----3' lambda exonuclease. t ...19863015880
fusarium oxysporum in red sea bream (pagrus sp.). 19863503145
specificity of fungal lipase in hydrolytic cleavage of oil.lipase isolated from the fungus fusarium oxysporum f. sp. lini has shown specificity to saturated acids. in vitro hydrolytic splitting of cotton seed, ground-nut and fungal (f. oxysporum) oil substrates by the enzyme preparation from this fungus, revealed preferential fatty acid specificity. the enzyme attacked the triglyceride molecule, liberating 90-97% of saturated fatty acids, irrespective of length of the carbon chain.19873439435
cutaneous infection caused by fusarium solani and fusarium oxysporum. 19873325538
synthesis and biological activity of isodithiobiurets, dithiobiurets, and dithiazoles.a series of isodithiobiurets, dithiobiurets, and dithiazoles was synthesized and tested for biological activity. generally, the compounds potentiated the hypnosis induced by pentobarbitone (50 mg/kg ip) in albino mice and exhibited antifungal and insecticidal activity against fusarium oxysporum and periplanata americana, respectively. some compounds showed anticonvulsant and analgesic activity in albino rats.19873508539
purification and characterization of a beta-galactosidase from fusarium oxysporum var. lini.beta-d-galactosidase was purified from a cellular extract of fusarium oxysporum var. lini by heat shock and successive chromatography on deae-cellulose de-52 and sephadex g-100. the purified enzyme was homogeneous on sds gel electrophoresis. it was inhibited by divalent cations such as zn++, mg++, and ca++. the michaelis constant and maximum velocity values for o-nitrophenyl beta-d-galacto-pyranoside were 6.76 mm and 816.7 mumol x mg protein-1 x min-1. the isoelectric point was 3.83, and the opt ...19873114341
the response of fusarium oxysporum to dithane m-45 and pseudomonas aeruginosa.the interaction of the fungicide dithane m-45 (active ingredient mancozeb), with pseudomonas aeruginosa strain not sensitive to the compound, was studied. pseudomonas aeruginosa produced a fungicidal compound inhibiting the growth of fusarium oxysporum. simultaneously pseudomonas aeruginosa degraded the ebis (ethyleneisothiocyaniane) formed from the spontaneous decomposition of the fungicide which is highly toxic for fusarium oxysporum. when the fungicide was used as seed dressing together with ...19872447753
mycoflora and trichothecene toxins of paddy grains from egypt.120 species and 38 genera were collected from 64 samples of paddy grains on glucose- and cellulose-czapek's agar at 28 degrees c. the total count of glycophilic and cellulose-decomposing fungi fluctuated between 216-29760; and 124-11320 colonies/g paddy grains on the two media, respectively. on glucose agar, the most common species were aspergillus niger, a. flavus, a. sydowi, a. terreus, a. fumigatus, a. ochraceus, penicillium chrysogenum, p. corylophilum, fusarium oxysporum, alternaria alterna ...19873696188
mycotoxins produced by fusarium oxysporum in the seeds of brassica campestris during storage.three recognized mycotoxins, viz. diaxetoxyscirpenol, t-2 toxin and zearalenone, along with the fatty esters of these toxins were isolated from the flask culture extractives of fusarium oxysporum schlecht (imi 273220) as also from seeds of brassica campestris var. sarson (mustard) infected with this fungus in storage. evaluation of biological activity and toxicity of the extractives and the effects of prolonged ingestion of the mouldy seeds by animals suggest that the infected seeds may present ...19872952882
mycoflora and vitamin content of sun-dried food condiments in nigeria.the fungi most frequently isolated (of 20-100% incidence) from fresh okra, pepper fruits and melon seeds are botryodiplodia theobromae, rhizopus stolonifer, trichoderma harzianum, mucor mucedo and fusarium oxysporum. the major mycoflora of the fruits which had been sun-dried for twenty days are comprised of aspergillus spp and penicillium spp which initially had formed the minor components (of less than 20% incidence) of the colonising population. the effect of sun-drying on the fruits was a sta ...19873507682
antibiotic activity of the pyrenocines.pyrenocine a, a phytotoxin produced by pyrenochaeta terrestris (hansen) gorenz, walker and larson, possesses general antibiotic activity against plants, fungi, and bacteria. effective doses for 50% inhibition (ed50s) are 4 micrograms/ml for onion seedling elongation; 14, 20, 20, and 25 micrograms/ml for the germination of asexual spores of fusarium oxysporum f. sp. cepae, fusarium solani f. sp. pisi, mucor hiemalis, and rhizopus stolonifer, respectively. pyrenocine a also inhibits the linear myc ...19873594310
growth and disease response of soybeans from early maturity groups to ozone and fusarium oxysporum.the single and combined effects of ozone (o(3)) and fusarium oxysporum on growth and disease expression of soybean genotypes differing in foliar sensitivity to o(3) were studied in the greenhouse. o(3) had no effect on root and hypocotyl rot severity of pi 153.283 (o(3)-sensitive, s) or pi 189.907 (o(3)-tolerant, t) maturity group i soybean lines. plants of both genotypes infected with f. oxysporum and exposed to o(3) had greater reductions in relative growth rate (rgr), net assimilation rate (n ...198715092691
the influence of various carbon and nitrogen sources on oil production by fusarium oxysporum.the oil-synthesizing capacity of fusarium oxysporum, cultivated on basal nutrient medium, was evaluated using different carbon and nitrogen sources. in one of the media, molasses was also used as a principal carbon source. media containing glucose and ammonium nitrate were found to be most efficient for oil production. fatty acid profile of the fungal oil indicated the presence of a wide range of fatty acids ranging from c8 to c24. fatty acid composition largely depends on the type of carbon and ...198718425683
in vitro selection of alfalfa plants resistant to fusarium oxysporum f. sp. medicaginis.from two lines of medicago sativa characterized by a high regeneration capability, calli resistant to culture filtrate of fusarium oxysporum f. sp. medicaginis have been selected. in these calli regeneration capability was greatly reduced and only one plant per callus was recovered. regenerated plants have been evaluated for resistance to culture filtrate and for in vivo resistance to the pathogen. three plants out of eight were resistant to the fungus and a high correlation between resistance t ...198724240327
fusarium wilt suppression and agglutinability of pseudomonas putida.mutants of pseudomonas putida (agg) that lack the ability to agglutinate with components present in washes of bean and cucumber roots showed limited potential to protect cucumber plants against fusarium oxysporum f. sp. cucumerinum. however, a higher level of protection was observed against fusarium wilt in cucumber plants coinoculated with the parental bacterium (agg), which was agglutinable. the agg mutants did not colonize the roots of cucumber plants as extensively as the agg parental isolat ...198816347713
chitosan-colloidal gold complexes as polycationic probes for the detection of anionic sites by transmission and scanning electron microscopy.a new cationic colloidal gold complex has been developed for ultrastructural localization of cell surface anionic sites by transmission and scanning electron microscopy. the marker is prepared by labelling gold particles of suitable sizes (6 to 70 nm in diameter) with chitosan, a polymer of beta (1----4)-linked d-glucosamine. using human red blood cells as a model, chitosan-gold complexes were shown to be specific for anionic sites and at ph 2 for sialic acid residues. the binding capacity of co ...19883063695
effects of cyclopropenoid fatty acids on fungal growth and lipid composition.cyclopropenoid fatty acids (cpe) isolated from sterculia foetida oil by urea clathration and reverse phase high performance liquid chromatography (hplc) were introduced into fungal cultures. stearate levels in phospholipids and triacylglycerols from ustilago maydis sporidia rose considerably in response to 30 microm cpe. in addition, cpe themselves were incorporated into glycerolipid fractions. sterol composition was unaffected. changes in lipid composition were accompanied by inhibition of dry ...19883131612
[cutaneous localizations of a fusarium oxysporum septicemia in a patient with bone marrow aplasia]. 19883165609
effect of antibiotics on synthesis of cell wall degrading enzymes by fusarium oxysporum and fusarium moniliforme. 19883170259
effect of herbicides on growth of some microscopic soil fungus species.the effect of 14 herbicides and two herbicide combinations of three microscopic soil fungus species was investigated by miller's method. (i) none of the 14 herbicides and 2 combinations belonging to four derivative groups induced the growth of aspergillus niger, fusarium oxysporum and rhizopus nigricans; (ii) higher doses of the herbicides prevented the growth of all test organisms significantly, the triasin derivative hungazin pk being the less and tiocarbamate derivatives alirox b 80 ec, aneld ...19883245414
disseminated cutaneous and peritoneal hyalohyphomycosis caused by fusarium species: three cases and review of the literature.three recent cases of hyalohyphomycosis caused by fusarium sp. illustrate differing aspects of infections produced by these organisms. one patient was undergoing continuous ambulatory peritoneal dialysis when peritonitis developed caused by fusarium moniliforme. removal of the catheter and amphotericin b were used in successful management. in a neutropenic patient on therapy for leukemia, multiple persistent infections occurred including jk diptheroids, and candida albicans sepsis. finally, nume ...19883278237
[hyperestrogenism in swine due to natural poisoning with zearalenone].a field case is described in which all prepuberal swine of a group of 20 pigs and 11 sows showed marked estrogenic effects. these consisted of enlarged mammary glands, swelled tumefacient vulva, and greatly enlarged internal reproductive organs. the corn used to feed these animals was found to contain 56 ppm zearalenone. deoxynivalenol (4.9 ppm) was found in the corn; t-2 toxin, nivalenol, fusarenon-x, diacetoxyscirpenol, aflatoxins and ochratoxins were absent. identity of z was confirmed by tlc ...19882976513
isolation and purification of a hemorrhagic factor (wortmannin) from fusarium oxysporum (n17b).an isolate of fusarium oxysporum schlecht, emend. synd. et hans. n17b isolated from a grassy area in lakselv, norway (arctic region) produced a toxin in culture when grown on rice in the laboratory. this new toxin, which was given the trivial name of h-1 (indicating hemorrhagic factor), caused toxic effects in rats, including food refusal, weight loss, hemorrhage in the stomach, intestines, heart, and thymus, and finally death. the uv spectrum of h-1 showed 210, 254, and 292 nm as absorption max ...19883389818
effect of streptomycin on extracellular proteases production by fusarium oxysporum. 19883076587
fusarium oxysporum keratitis.we describe herein a patient with a rare occurrence of mycotic keratitis caused by fusarium oxysporum. the fungus destroyed the cornea, and a perforating corneal graft had to be performed. the drug of choice in such diagnosed cases is natamycin. a comparison between fusarium oxysporum and fusarium solani is discussed.19892919034
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