Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| antifungal activities of triterpenoids from the roots of astilbe myriantha diels. | during a search for plant extracts that possess activity against the fungi that cause diseases in plants, a 95% ethanol extract from the roots of astilbe myriantha diels showed inhibitory activity against colletotrichum gloeosporioides. bioassay-guided fractionation of the 95% ethanol extract led to the isolation of seven triterpenoids (1-7). their structures were elucidated by spectroscopy and by a comparison with literature data. among these compounds, 3β,6β,24-trihydroxyurs-12-en-27-oic acid ... | 2011 | 25212161 |
| fungal infections among diabetic foot ulcer- patients attending diabetic clinic in kenyatta national hospital, kenya. | to isolate and identify fungal pathogens associated with dermatophytoses in diabetic patients and identify the spectrum of yeasts colonising diabetic foot ulcers at kenyatta national hospital. | 2011 | 24968597 |
| molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran. | fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ... | 2011 | 23961146 |
| inactivation of snt2, a bah/phd-containing transcription factor, impairs pathogenicity and increases autophagosome abundance in fusarium oxysporum. | the soil-borne, asexual fungus fusarium oxysporum f.sp. melonis (fom) is a causal agent of muskmelon wilt disease. the current study focused on the most virulent race of fom-race 1,2. the tagged mutant d122, generated by agrobacterium tumefaciens-mediated transformation, caused the delayed appearance of initial wilt disease symptoms, as well as a 75% reduction in pathogenicity. d122 was impaired in the gene product homologous to the snt2-like transcription factor of schizosaccharomyces pombe. in ... | 2011 | 21535351 |
| characterization of plant-growth-promoting traits of acinetobacter species isolated from rhizosphere of pennisetum glaucum. | a total of 31 acinetobacter isolates were obtained from the rhizosphere of pennisetum glaucum and evaluated for their plant-growth-promoting traits. two isolates, namely acinetobacter sp. pucm1007 and a. baumannii pucm1029, produced indole acetic acid (10-13 microgram/ml). a total of 26 and 27 isolates solubilized phosphates and zinc oxide, respectively. among the mineral-solubilizing strains, a. calcoaceticus pucm1006 solubilized phosphate most efficiently (84 mg/ml), whereas zinc oxide was sol ... | 2011 | 21715961 |
| innate immune responses of a scleractinian coral to vibriosis. | scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are amongst the most diverse of all ecosystems. over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographical widespread and a major threat to this ecosystem. although coral immunity has been the subject of increasing study, the available ... | 2011 | 21536670 |
| indicator organisms for assessing sanitization during composting of plant wastes. | the potential for using plant pathogens and seeds as indicator organisms for assessing sanitization of plant wastes during composting was tested in bench-scale flask and large-scale systems. plasmodiophora brassicae was unsuitable due to high temperature tolerance in dry to moist composts, and detection of viable inoculum post-composting using bioassay plants not corresponding with that using taqman® pcr, possibly due to preservation of nucleic acids at elevated temperatures. several other plant ... | 2011 | 21546235 |
| identification and evaluation of two diagnostic markers linked to fusarium wilt resistance (race 4) in banana (musa spp.). | fusarium wilt caused by the fungus fusarium oxysporum f. sp. cubense race 4 (foc4) results in vascular tissue damage and ultimately death of banana (musa spp.) plants. somaclonal variants of in vitro micropropagated banana can hamper success in propagation of genotypes resistant to foc4. early identification of foc4 resistance in micropropagated banana plantlets is difficult, however. in this study, we identified sequence-characterized amplified region (scar) markers of banana associated with re ... | 2011 | 21547366 |
| Cloning of novel cellulases from cellulolytic fungi: Heterologous expression of a family 5 glycoside hydrolase from Trametes versicolor in Pichia pastoris. | Total cDNA isolated from cellulolytic fungi cultured in cellulose was examined for the presence of sequences encoding for endoglucanases. Novel sequences encoding for glycoside hydrolases (GHs) were identified in Fusarium oxysporum, Ganoderma applanatum and Trametes versicolor. The cDNA encoding for partial sequences of GH family 61 cellulases from F. oxysporum and G. applanatum shares 58 and 68% identity with endoglucanases from Glomerella graminicola and Laccaria bicolor, respectively. A new G ... | 2011 | 22142722 |
| Characterization of five fungal endophytes producing Cajaninstilbene acid isolated from pigeon pea [Cajanus cajan (L.) Millsp]. | Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L) among the endophytes tested after incubation for five days. Both morphological ... | 2011 | 22102911 |
| Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Cultures by Oligosaccharides from Its Endophytic Fungus Fusarium oxysporum Dzf17. | The effects of the oligosaccharides from the endophytic fungus Fusarium oxysporum Dzf17 as elicitors on diosgenin production in cell suspension cultures of its host Dioscorea zingiberensis were investigated. Three oligosaccharides, DP4, DP7 and DP10, were purified from the oligosaccharide fractions DP2-5, DP5-8 and DP8-12, respectively, which were prepared from the water-extracted mycelial polysaccharide of the endophytic fungus F. oxysporum Dzf17. When the cell cultures were treated with fracti ... | 2011 | 22183887 |
| constitutive expression, purification and characterization of a phosphoglucomutase from fusarium oxysporum. | the phosphoglucomutase gene from a wild type fusarium oxysporum strain (f3), was homologously expressed, under the control of the constitutive promoter of gpda of aspergillus nidulans. the transformant produced elevated levels of phosphoglucomutase activity compared to the wild type, a fact that facilitated the subsequent purification procedure. the enzyme (fopgm) was purified to homogeneity applying three anion exchange and one gel filtration chromatography steps. the native enzyme revealed a m ... | 2011 | 22112903 |
| longitudinal melanonychia caused by fusarium oxysporum in immunocompetent patient. | 2011 | 22074386 | |
| studies on fungal cultural filtrates against adult culex quinquefasciatus (diptera: culicidae) a vector of filariasis. | entomopathogenic fungi have significant potential to control mosquito population. the culture filtrates of fusarium oxysporum, lagenidium giganteum, trichophyton ajelloi, and culicinomyces clavisporus were evaluated against adults of cx. quinquefasciatus. the culture filtrates were obtained by filtering the broth through whatman-1 filter paper. these culture filtrates of c. clavisporus have been found significantly pathogenic with lc(50)-2.5, lc(90)-7.24, and lc(99)-8.7 ml, respectively, after e ... | 2011 | 22132305 |
| a resource for the in silico identification of fungal polyketide synthases from predicted fungal proteomes. | the goal of this study was to develop a tool specifically designed to identify iterative polyketide synthases (ipkss) from predicted fungal proteomes. a fungi-based pks prediction model, specifically for fungal ipkss, was developed using profile hidden markov models (phmms) based on two essential ipks domains, the β-ketoacyl synthase (ks) domain and acyltransferase (at) domain, derived from fungal ipkss. this fungi-based pks prediction model was initially tested on the well-annotated proteome of ... | 2011 | 22112245 |
| synthetic molecular mimics of naturally occurring cyclopentenones exhibit antifungal activity towards pathogenic fungi. | the naturally occurring reactive electrophilic species 12-oxo-phytodienoic acid (12-oxo-pda) is a potent antifungal agent, whereas the plant growth regulator jasmonic acid, which is synthesized from 12-oxo-pda, is ineffective. to address what structural features of the molecule endow it with antifungal activity, we synthesized a series of molecular mimics of 12-oxo-pda varying in the length of the alkyl chain at its c-4 ring position. the octyl analogue (4-octyl cyclopentenone) was the most effe ... | 2011 | 21921102 |
| molecular identification of two vegetative compatibility groups of fusarium oxysporum f. sp. cepae. | fusarium oxysporum f. sp. cepae (focep), which causes basal rot of onions, consists of seven vegetative compatibility groups (vcgs 0420 to 0426) and several single-member vcgs (smvs). focep populations in south africa and colorado each comprise one main vcg, namely vcg 0425 and 0421, respectively. the aim of this study was to develop sequence-characterized amplified region (scar) markers for the identification of vcgs 0425 and 0421, using 79 previously characterized f. oxysporum isolates. a seco ... | 2011 | 21970568 |
| synthesis of pyrazolo[3,4-b]pyridines under microwave irradiation in multi-component reactions and their antitumor and antimicrobial activities - part 1. | an efficient one-pot synthesis in multi-component system (mrcs) for the preparation of pyrazolo[3,4-b]pyridine derivatives from the reaction of 5-amino-1-phenyl-3-(pyridin-3-yl)-1h-pyrazole with 4-anisaldehyde and p-substituted β-ketonitriles or with pyruvic acid and some aromatic aldehydes in acetic acid medium. the reactions were carried out by two different techniques, conventional heating and microwave irradiation. these compounds were screened for their antibacterial activity against gram-p ... | 2011 | 22178093 |
| phylogeny and pathogenicity of fusarium oxysporum isolates from cottonseed imported from australia into california for dairy cattle feed. | a unique biotype of the fusarium wilt pathogen, fusarium oxysporum schlecht. f.sp. vasinfectum (atk) sny. & hans., found in australia in 1993 is favored by neutral or alkaline heavy soils and does not require plant parasitic nematodes to cause disease. this makes it a threat to 4-6 million acres of usa upland cotton ( gossypium hirsutum l.) that is grown on heavy alkaline soil and currently is not affected by fusarium wilt. in 2001-2002, several shiploads of live cottonseed were imported into ca ... | 2011 | 22004096 |
| expression and functional analysis of two osmotin (pr5) isoforms with differential antifungal activity from piper colubrinum: prediction of structure-function relationship by bioinformatics approach. | osmotin, a pathogenesis-related antifungal protein, is relevant in induced plant immunity and belongs to the thaumatin-like group of proteins (tlps). this article describes comparative structural and functional analysis of the two osmotin isoforms cloned from phytophthora-resistant wild piper colubrinum. the two isoforms differ mainly by an internal deletion of 50 amino acid residues which separates them into two size categories (16.4 kda-pcosm1 and 21.5 kda-pcosm2) with pi values 5.6 and 8.3, r ... | 2011 | 22207456 |
| genetic diversity of fusarium oxysporum f.sp. cubense isolates (foc) of india by inter simple sequence repeats (issr) analysis. | to find out the genetic diversity of indian foc isolates of banana, a total of 107 isolates of fusarium which includes 98 foc isolates obtained from different banana growing regions of india and seven foc isolates belong to all known vcgs obtained from australia and two non-pathogenic fusarium oxysporum (npfo) isolates were subjected to issr analysis. in the initial screening of issr primers, out of 34, 10 primers which generated more polymorphic bands were selected for further analysis. the phy ... | 2011 | 21983913 |
| systemically induced resistance and microbial competitive exclusion: implications on biological control. | the root-knot nematode, meloidogyne incognita, is among the most damaging agricultural pests, particularly to tomato. the mutualistic endophytes fusarium oxysporum strain fo162 (fo162) and rhizobium etli strain g12 (g12) have been shown to systemically induce resistance towards m. incognita. by using triple-split-root tomato plants, spatially-separated but simultaneous inoculation of both endophytes did not lead to additive reductions in m. incognita infection. more importantly, spatially-separa ... | 2011 | 21899390 |
| comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens. | the vascular wilt fungi verticillium dahliae and v. albo-atrum infect over 200 plant species, causing billions of dollars in annual crop losses. the characteristic wilt symptoms are a result of colonization and proliferation of the pathogens in the xylem vessels, which undergo fluctuations in osmolarity. to gain insights into the mechanisms that confer the organisms' pathogenicity and enable them to proliferate in the unique ecological niche of the plant vascular system, we sequenced the genomes ... | 2011 | 21829347 |
| the frp1 f-box gene has different functions in sexuality, pathogenicity and metabolism in three fungal pathogens. | plant-pathogenic fungi employ a variety of infection strategies; as a result, fungi probably rely on different sets of proteins for successful infection. the f-box protein frp1, only present in filamentous fungi belonging to the sordariomycetes, leotiomycetes and dothideomycetes, is required for nonsugar carbon catabolism and pathogenicity in the root-infecting fungus fusarium oxysporum. to assess the role of frp1 in other plant-pathogenic fungi, frp1 deletion mutants were generated in fusarium ... | 2011 | 21722294 |
| Different active biomolecules involved in biosynthesis of gold nanoparticles by three fungus species. | In this paper, the intracellular gold nanoparticles were biosynthesized using three fungi including Aureobasidium pullulans (A. pullulans), Fusarium sp. and Fusarium oxysporum (F. oxysporum) after immersion the fungal cells in AuCl4- ions solution. UV-vis and FTIR spectrum, and biochemical compositions analysis of Au nano-fungal cells suggested that active biomolecules of reducing sugar of A. pullulans, and proteins in Fusarium sp. and F. oxysporum were tested positive of providing the function ... | 2011 | 21702362 |
| purification and characterization of an extracellular 24 kda chitobiosidase from the mycoparasitic fungus trichoderma saturnisporum. | a trichoderma saturnisporum hamill isolate gitx-panog (c) exhibiting strong chitinolytic and antifungal activity against fusarium oxysporum f.sp. dianthi, the causal agent of vascular wilt in carnation was used to purify extracellular chitobiosidase using czapek-dox broth amended with the fungal mycelium as the carbon source. the protein was purified by precipitation with ammonium sulphate, followed by deae-cellulose anion-exchange and sephacryl s-200 high resolution gel filtration chromatograph ... | 2011 | 21953631 |
| evaluation of luminex xtag fungal analyte-specific reagents for rapid identification of clinically relevant fungi. | invasive fungal infections (ifi) remain a serious threat to immunocompromised hosts. current diagnostic methods, including fungal culture and antigen detection, are slow and often lack specificity. rapid diagnostic tools with increased sensitivity and specificity could improve the care of patients with ifi. recently, luminex molecular diagnostics (toronto, canada) developed 23 analyte-specific reagents (asrs) for the detection of the most common clinically relevant fungi. this study's objective ... | 2011 | 21880976 |
| [Identification of soil-borne fungi using Fourier transform infrared spectroscopy]. | Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium monili forme, Fusarium semitectum, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from ... | 2011 | 22007392 |
| GMC oxidoreductase, a highly expressed protein in a potent biocontrol agent Fusarium oxysporum Cong:1-2, is dispensable for biocontrol activity. | A spontaneous non-pathogenic variant (Cong:1-2) derived from Fusarium oxysporum f. sp. conglutinans (Cong: 1-1), a causal agent of cabbage yellows, carries biocontrol activity for cabbage yellows. We found a GMC oxidoreductase (ODX1) among the proteins expressed much more in Cong:1-2 than Cong:1-1 by 2D-DIGE comparison. GMC oxidoreductases have been reported to be involved in biocontrol activity of several plant pathogenic fungi. The gene encoding ODX1 in Cong:1-2 was cloned, and targeted disrup ... | 2011 | 21914969 |
| Three new compounds from soil actinomycete Streptomyces albospinus 15-4-2. | Three new compounds, 2-methyl-2,5,6-bornantriol (1), 4,4'-(3-hydroxypropane-1,1-diyl)diphenol (2), and 7-(4-methoxybenzyl)-4,5,6,7-tetrahydro-1,3-oxazepine-5,6-diol (3), were isolated from the fermentation broth of the soil actinomycete Streptomyces albospinus 15-4-2. Their structures were completely elucidated using the combination of 1D, 2D NMR techniques (COSY, HMQC, HMBC, and ROESY), and HR-ESI-MS analysis. None of the compounds 1-3 showed any inhibitory effect on Fusarium oxysporum f.sp. cu ... | 2011 | 21972804 |
| effects of (14)c-labelled precursor feeding on production of beauvericin, enniatins h, i, and mk1688 by fusarium oxysporum kfcc11363p. | the effects of (14)c-labelled precursor feeding on the production of cyclic hexadepsipeptides were investigated by the mycelium of f. oxysporum kfcc11363p producing beauvericin along with enniatins h, i, and mk1688. most (14)c-phenylalanine and (14)c-valine were incorporated easily into the biosynthetic pathway of (14)c-labelled beauvericin in vivo by the mycelium. however, only a small amount of (14)c-labelled enniatins could be detected by feeding of (14)c-valine. when l-valine was fed as a pr ... | 2011 | 22019403 |
| galleria mellonella as model host for the trans-kingdom pathogen fusarium oxysporum. | fusarium oxysporum, the causal agent of vascular wilt disease, affects a wide range of plant species and can produce disseminated infections in humans. f. oxysporum f. sp. lycopersici isolate fgsc 9935 causes disease both on tomato plants and immunodepressed mice, making it an ideal model for the comparative analysis of fungal virulence on plant and animal hosts. here we tested the ability of fgsc 9935 to cause disease in the greater wax moth galleria mellonella, an invertebrate model host that ... | 2011 | 21907298 |
| The antifungal, cytotoxic, antitermite and insecticidal activities of Zizyphus jujube. | Plants are very useful, self-generating machines, producing a variety of useful bioactive products. Keeping in view this idea, the crude methanolic extract and various fractions of Zizyphus jujuba were screened for antifungal, cytotoxic, antitermite and insecticidal activities. Low activity was shown by the crude methanolic extract (12%), n-hexane (9%), chloroform (20%) and ethyl acetate (14%) fraction against Penicillium notatum. Low activity was shown by the n-hexane fraction against Aspergill ... | 2011 | 21959810 |
| characterization of the gene encoding pisatin demethylase (fopda1) in fusarium oxysporum. | the pea pathogen fusarium oxysporum f. sp. pisi is able to detoxify pisatin produced as a defense response by pea, and the gene encoding this detoxification mechanism, fopda1, was 82% identical to the cytochrome p450 pisatin demethylase pda1 gene in nectria haematococca. a survey of f. oxysporum f. sp. pisi isolates demonstrated that, as in n. haematococca, the pda gene of f. oxysporum f. sp. pisi is generally located on a small chromosome. in n. haematococca, pda1 is in a cluster of pea pathoge ... | 2011 | 22066900 |
| antifungal metabolites from the roots of diospyros virginiana by overpressure layer chromatography. | a preparative overpressure layer chromatography (oplc) method was successfully used for the separation of two new natural compounds, 4-hydroxy-5,6-dimethoxynaphthalene-2-carbaldehyde (1) and 12,13-didehydro-20,29-dihydrobetulin (2) together with nine known compounds, including 7-methyljuglone (3), diospyrin (4), isodiospyrin (5), shinanolone (6), lupeol (7), betulin (8), betulinic acid (9), betulinaldehyde (10), and ursolic acid (11) from the acetone extract of the roots of diospyros virginiana. ... | 2011 | 22162171 |
| Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113. | Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) inc ... | 2011 | 21972146 |
| expression of rice thaumatin-like protein gene in transgenic banana plants enhances resistance to fusarium wilt. | the possibility of controlling fusarium wilt-caused by fusarium oxysporum sp. cubensec (race 4)-was investigated by genetic engineering of banana plants for constitutive expression of rice thaumatin-like protein (tlp) gene. transgene was introduced to cauliflower-like bodies' cluster, induced from meristemic parts of male inflorescences, using particle bombardment with plasmid carrying a rice tlp gene driving by the camv 35s promoter. hygromycin b was used as the selection reagent. the presence ... | 2011 | 22183565 |
| Arsenic biotransformation by arsenic-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1. | Bioremediation of arsenic (As)-contaminated soil using microorganisms has been a focus of research because it is environment friendly and cost-effective. The As-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were exposed to 50 mg l(-1) of As(V), and the biotransformation of As and the concomitant variance of Eh and pH in the media were studied. Fresh weights of all three isolates increased and then decreased depended on cultivation ... | 2011 | 21920586 |
| a highly conserved effector in fusarium oxysporum is required for full virulence on arabidopsis. | secreted-in-xylem (six) proteins of the vascular wilt pathogen fusarium oxysporum f. sp. lycopersici are secreted during infection of tomato and function in virulence or avirulence. f. oxysporum formae speciales have specific host ranges but the roles of six proteins in diverse hosts are unknown. we identified homologs of f. oxysporum f. sp. lycopersici six1, six4, six8, and six9 in the genome of arabidopsis infecting isolate fo5176. a six4 homolog (termed fo5176-six4) differed from that of f. o ... | 2011 | 21942452 |
| Peptidotriazoles with antimicrobial activity against bacterial and fungal plant pathogens. | We designed and prepared peptidotriazoles based on the antimicrobial peptide BP100 (LysLysLeuPheLysLysIleLeuLysTyrLeu-NH(2)) by introducing a triazole ring in the peptide backbone or onto the side chain of a selected residue. These compounds were screened for their in vitro growth inhibition of bacterial and fungal phytopathogens, and for their cytotoxic effects on eukaryotic cells and tobacco leaves. Their proteolytic susceptibility was also analyzed. The antibacterial activity and the hemolysi ... | 2011 | 22198367 |
| structural characterization of the o-chain polysaccharide from an environmentally beneficial bacterium pseudomonas chlororaphis subsp. aureofaciens strain m71. | pseudomonas chlororaphis subsp. aureofaciens strain m71 was isolated from the root of a tomato plant and it was able to control in vivo fusarium oxysporum f. sp. radicis-lycopersici responsible for the tomato crown and root rot. recently, strain m71 was evaluated even for its efficacy in controlling seiridium cardinale, the causal agent of bark canker of common cypress (cupressus sempervirens l.). strain m71 ability to persist on the tomato rhizosphere and on the aerial part of cypress plants co ... | 2011 | 22036123 |
| a comparative in silico analysis on frequency and distribution of microsatellites in coding regions of three formae speciales of fusarium oxysporum and development of est-ssr markers for polymorphism studies. | fusarium oxysporum is a ubiquitous species complex of soil-borne plant pathogens comprising of many different formae speciales, each characterized by a high degree of host specificity. in the present investigation, we surveyed microsatellites in the available express sequence tags and transcript sequences of three formae speciales of f. oxysporum viz. melonis (fom), cucumerium (foc), and lycopersici (fol). the relative abundance and density of microsatellites were higher in fom when compared wit ... | 2011 | 22171944 |
| Application of PCR-Denaturing-Gradient Gel Electrophoresis (DGGE) Method to Examine Microbial Community Structure in Asparagus Fields with Growth Inhibition due to Continuous Cropping. | Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still controversial. We aimed to develop a technique for the biological field diagnosis of growth inhibition due to continuous cropping. Therefore, in this study, fungal community structure ... | 2011 | 22200640 |
| rho1 and other gtp-binding proteins are associated with vesicles carrying glucose oxidase activity from fusarium oxysporum f. sp. lycopersici. | in eucaryotic cells, the delivery of a secreted protein to the plasma membrane via vesicles must include transport, recognition, and fusion events. proteins exposed on the cytoplasmic face of the secretory vesicles play a role in these events; these include the gtp-binding proteins, which are crucial components in this process. fractions enriched with vesicles carrying glucose oxidase (gox) activity from fusarium oxysporum f. sp. lycopersici, a soilborne fungal pathogen causing vascular wilt on ... | 2011 | 21203842 |
| isolation and characterization of an exopolygalacturonase from fusarium oxysporum f.sp. cubense race 1 and race 4. | fusarium wilt is an economically devastating disease that affects banana production. although cavendish banana cultivars are resistant to fusarium oxysporum f.sp. cubense race 1 (foc1) and maitain banana production after gros michel was destructed by race 1, a new race race 4 (foc4) was found to infect cavendish. | 2011 | 21920035 |
| identification and functional characterization of indole-3-acetamide-mediated iaa biosynthesis in plant-associated fusarium species. | the plant hormone indole-3-acetic acid (iaa) can be synthesized from tryptophan via the intermediate indole-3-acetamide (iam). the two genes, iaam (encoding tryptophan monooxygenase) and iaah (encoding indole-3-acetamide hydrolase) that constitute the iam pathway have been described in plant-associated bacteria. we have identified putative homologs of the bacterial iaam and iaah genes in four fusarium species -fusarium proliferatum, fusarium verticillioides, fusarium fujikuroi, and fusarium oxys ... | 2011 | 22079545 |
| identification of limiting factors for the optimum growth of fusarium oxysporum in liquid medium. | fusarium oxysporum is a highly ubiquitous species that infects a wide range of hosts causing various diseases such as vascular wilts, yellows, rots, and damping-off. despite the immense economic significance of this phytopathogen, few workers have reported growth studies in this genus in submerged culture. in the present study, several parameters such as change in media ph, biomass, pattern of substrate utilization, viability of the fungal cells, and protein content were observed over a period o ... | 2011 | 21976815 |
| direct hydride shift mechanism and stereoselectivity of p450(nor) confirmed by qm/mm calculations. | nitric oxide reductase (p450(nor)) found in fusarium oxysporum catalyzes the reduction of nitric oxide to n(2)o in a multistep process. the reducing agent, nadh, is bound in the distal pocket of the enzyme, and direct hydride transfer occurs from nadh to the nitric oxide bound heme enzyme, forming intermediate i. here we studied the possibility of hydride transfer from nadh to both the nitrogen and oxygen of the heme-bound nitric oxide, using quantum chemical and combined quantum mechanics/mol ... | 2011 | 22148861 |
| ERK1/2 and AKT pathway were activated during (3R,6R)-Bassiatin (1)-induced apoptosis in MCF-7 cells. | (3R,6R)-bassiatin(1) was isolated from the endogenous fungus Fusarium oxysporum J8-1-2. Our previous studies show that (3R,6R)-bassiatin(1) is a novel antiestrogen agent which is cytotoxic to estrogen receptor (ER)-positive breast cancer MCF-7 cells via cell cycle arrest and induction of apoptosis. (3R,6R)-Bassiatin(1) could suppress the protein and mRNA expression of estrogen receptor (ERa) and estrogen responsive gene cyclinD1 and PR leading to cell cycle arrest and apoptosis. In the present ... | 2011 | 22150072 |
| the presence of fungi on contact electrical stimulation electrodes and ultrasound transducers in physiotherapy clinics. | to evaluate the presence of fungi on contact electrodes and ultrasound transducers from physiotherapy clinics. | 2011 | 22051582 |
| Cloning and characterization of a bifunctional glycosyl hydrolase from an antagonistic Pseudomonas putida strain P3(4). | A fluorescent pseudomonad strain P3(4) showing chitinolysis on chitinase detection agar and antagonism against Fusarium oxysporum f.sp dianthi causing vascular wilt of carnation was isolated from pea rhizosphere soil. PCR primers specific for glycosyl hydrolase family 5 (GH5) of Pseudomonas putida isolate KT2440 amplified a 947 bp fragment of the GH5 gene from P3(4). Cloning of this gene into Escherichia coli M15 using an expression vector pQE-30UA and screening on chitin and chitosan detection ... | 2011 | 21953214 |
| [the effect of the formulation on the shelf-life of biopesticides based on colombian isolates of trichoderma koningiopsis th003 and trichoderma asperellum th034.] | background: four biopesticide prototypes formulated as dispersible granules and dry powders based on 2 colombian isolates of trichoderma koningiopsis (th003) and t. asperellum (th034) were developed. these microorganisms have antagonist activity against fusarium oxysporum f. sp. lycopersici and rhizoctonia solani with a reduction in incidence of between 70 and 100% in tomato crops and potato crops, respectively. aim: to determine the effect of the formulation on the shelf-life of 4 biopesticides ... | 2011 | 22116019 |
| salicylic acid is a modulator of catalase isozymes in chickpea plants infected with fusarium oxysporum f. sp. ciceri. | the relationship between salicylic acid level catalases isoforms chickpea cv. iccv-10 infected with fusarium oxysporum f. sp. ciceri was investigated. pathogen-treated chickpea plants showed high levels of sa compared with the control. two isoforms of catalases in shoot extract (cat-is and cat-iis) and single isoform in root extract (cat-r) were detected in chickpea. cat-is and cat-r activities were inhibited in respective extracts treated with pathogen whereas, cat-iis activity was not inhibite ... | 2011 | 22245913 |
| biocontrol potential of siderophore producing heavy metal resistant alcaligenes sp. and pseudomonas aeruginosa rzs3 vis-à-vis organophosphorus fungicide. | in present study in vitro phytopathogen suppression activity of siderophoregenic preparations of ni and mn resistant alcaligenes sp. stc1 and pseudomonas aeruginosa rzs3 sh-94b isolated from soil were found superior over the chemical pesticide. siderophore rich culture broth and siderophore rich supernatant exerted antifungal activity against aspergillus niger ncim 1025, aspergillus flavus ncim 650, fusarium oxysporum ncim 1281, alternaria alternata ari 715, cercospora arachichola, metarhizium a ... | 2011 | 22754001 |
| identification and biocontrol efficacy of streptomyces miharaensis producing filipin iii against fusarium wilt. | a number of bacterial strains were isolated from the internal tissue of trapa japonica. of these, strain kpe62302h, which had a 16s rdna sequence identical to that of streptomyces miharaensis showed antifungal activity against several plant pathogens. treatment of seeds with strain kpe62302h induced a significant reduction in the incidence of fusarium wilt in tomato plants compared with untreated controls. an antifungal substance (fp-1) was purified from the culture extract of strain kpe62302h u ... | 2011 | 22460913 |
| evaluation of plant-growth-promoting rhizobacteria, acibenzolar-s-methyl and hymexazol for integrated control of fusarium crown and root rot on tomato. | plant growth-promoting rhizobacteria (pgpr) can be potential agents for biological control of plant pathogens, while their combined use with conventional pesticides may increase their efficacy and broaden the disease control spectrum. the effect of four different bacillus sp. pgpr strains (b. subtilis gb03 and fzb24, b. amyloliquefaciens in937a and b. pumilus se34) applied individually and in mixtures, as well as in combined use with acibezolar-s-methyl (asm) and hymexazol, on plant growth promo ... | 2011 | 22307860 |
| phylogenomic and functional domain analysis of polyketide synthases in fusarium. | fusarium species are ubiquitous in nature, cause a range of plant diseases, and produce a variety of chemicals often referred to as secondary metabolites. although some fungal secondary metabolites affect plant growth or protect plants from other fungi and bacteria, their presence in grain-based food and feed is more often associated with a variety of diseases in plants and in animals. many of these structurally diverse metabolites are derived from a family of related enzymes called polyketide s ... | 2011 | 22289777 |
| biotic and abiotic stress tolerance in transgenic tomatoes by constitutive expression of s-adenosylmethionine decarboxylase gene. | recent findings have implicated the role of polyamines (putrescine, spermidine and spermine) in stress tolerance. therefore, the present work was carried out with the goal of generating transgenic tomato plants with human s-adenosylmethionine decarboxylase (samdc) gene, a key gene involved in biosynthesis of polyamines, viz. spermidine and spermine and evaluating the transgenic plants for tolerance to both biotic and abiotic stresses. several putative transgenic tomato plants with normal phenoty ... | 2011 | 23573001 |
| [anaerobic growth ability and alcohol fermentation activity of microscopic fungi]. | the method proposed in this study was used to isolate fungi grown under anaerobic conditions and to reveal distinctions in their abundance and species composition in different habitats. the ability of micromycetes of different taxa to grow under anaerobic conditions and ensure alcohol fermentation was determined for a representative sample (344 strains belonging to more than 60 species). the group of fungi growing under anaerobic conditions included species with high, moderate, and low fermentat ... | 2011 | 22808743 |
| enhancing effect of shimizuomyces paradoxus on seed germination and seedling growth of canola, plant growth of cucumber, and harvest of tomato. | shimizuomyces paradoxus showed no inhibitory effect against plant pathogen fungi, such as fusarium oxysporum f. sp. lycopersici and alternaria solani. the s. paradoxus culture filtrate showed higher seed germination and seedling growth rates in canola than distilled water and potato-dextrose broth. a conidial suspension of 1.0×10(4)/ml resulted in the highest growth stimulating effects on total plant length, and fresh and dry weight of shoots and roots in cucumber, when compared to the highest s ... | 2011 | 22783066 |
| inhibitory effects of essential oils of medicinal plants from growth of plant pathogenic fungi. | plant cells produce a vast amount of secondary metabolites. production of some compounds is restricted to a single species. some compounds are nearly always found only in certain specific plant organs and during a specific developmental period of the plant. some secondary metabolites of plants serve as defensive compounds against invading microorganisms. nowadays, it is attempted to substitute the biological and natural agents with chemically synthesized fungicides. in the present research, the ... | 2011 | 22702190 |
| transformation of pwwo in rhizobium leguminosarum dpt to engineer toluene degrading ability for rhizoremediation. | rhizoremediation of organic xenobiotics is based on interactions between plants and their associated micro-organisms. the present work was designed to engineer a bacterial system having toluene degradation ability along with plant growth promoting characteristics for effective rhizoremediation. pwwo harboring the genes responsible for toluene breakdown was isolated from pseudomonas putida mtcc 979 and successfully transformed in rhizobium dpt. this resulted in a bacterial strain (dpt(t)) which h ... | 2011 | 23729882 |
| in vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens. | the aim of this study was to examine the effect of five naturally occurring compounds from essential oils on 10 different species of mycotoxigenic fungi involved in several plant diseases. the antifungal activities of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol were observed in vitro on fusarium subglutinans, fusarium cerealis, fusarium verticillioides, fusarium proliferatum, fusarium oxysporum, fusarium sporotrichioides, aspergillus tubingensis, aspergillus carbonarius, ... | 2011 | 22257275 |
| arbuscular mycorrhizal fungal spores host bacteria that affect nutrient biodynamics and biocontrol of soil-borne plant pathogens. | the aim of this research was to isolate and characterize bacteria from spores of arbuscular mycorrhizal fungi (amf). we designated these bacteria 'probable endobacteria' (pe). three bacterial strains were isolated from approximately 500 spores of gigaspora margarita (becker and hall) using a hypodermic needle (diameter, 200 μm). the bacteria were identified by morphological methods and on the basis of ribosomal gene sequences as bacillus sp. (ktcigm01), bacillus thuringiensis (ktcigm02), and pae ... | 2011 | 23213368 |
| structural and biochemical changes in salicylic-acid-treated date palm roots challenged with fusarium oxysporum f. sp. albedinis. | histochemical and ultrastructural analyses were carried out to assess structural and biochemical changes in date palm roots pretreated with salicylic acid (sa) then inoculated with fusarium oxysporum f. sp. albedinis (foa). flavonoids, induced proteins, and peroxidase activity were revealed in root tissues of sa-treated plants after challenge by foa. these reactions were closely associated with plant resistance to foa. host reactions induced after inoculation of sa-treated plants with foa includ ... | 2011 | 22567327 |
| purification and characterization of a cktlp protein from cynanchum komarovii seeds that confers antifungal activity. | cynanchum komarovii al iljinski is a desert plant that has been used as analgesic, anthelminthic and antidiarrheal, but also as a herbal medicine to treat cholecystitis in people. we have found that the protein extractions from c. komarovii seeds have strong antifungal activity. there is strong interest to develop protein medication and antifungal pesticides from c. komarovii for pharmacological or other uses. | 2011 | 21364945 |
| functional characterization of the recombinant antimicrobial peptide trx-ace-amp1 and its application on the control of tomato early blight disease. | ace-amp1 is a potent antifungal peptide found in onion (allium cepa) seeds with sequence similarity to plant lipid transfer proteins. transgenic plants over-expressing ace-amp1 gene have enhanced disease resistance to some fungal pathogens. however, mass production in heterologous systems and in vitro application of this peptide have not been reported. in this study, ace-amp1 was highly expressed in a prokaryotic escherichia coli system as a fusion protein. the purified protein inhibited the gro ... | 2011 | 21380518 |
| interactions of arabidopsis and m. truncatula with the same pathogens differ in dependence on ethylene and ethylene response factors. | microbial pathogens inflict large losses to agriculture annually and thus mechanisms of plant resistance and how to deploy them to enhance disease resistance in crops are foci of much research interest. we recently described the important role of ethylene and ethylene response transcription factors (erfs), particularly mterf1-1, in mediating resistance to the fungal pathogen rhizoctonia solani in the model legume, medicago truncatula. previous studies on the closely related aterf14, a master reg ... | 2011 | 21389781 |
| heterologous expression of transaldolase gene tal from saccharomyces cerevisiae in fusarium oxysporum for enhanced bioethanol production. | the filamentous fungus fusarium oxysporum is known for its ability to ferment xylose-producing ethanol. however, efficiency of xylose utilization and ethanol yield was low. in this study, the transaldolase gene from saccharomyces cerevisiae has been successfully expressed in f. oxysporum by an agrobacterium tumefaciens-mediated transformation method. the enzymatic activity of the recombinant fungus (cs28pcam-sctal4) was 0.195 times higher than that of the wild-type strain (cs28). the recombinant ... | 2011 | 21394668 |
| cloning and optimized expression of a gh-11 xylanase from fusarium oxysporum in pichia pastoris. | the endo-1,4-ß-xylanase gene xyn11a from fusarium oxysporum, member of the fungal glycosyl hydrolase (gh) family 11, was cloned and expressed in pichia pastoris. the mature xylanase gene, which generates after the excision of one intron and the secreting signal peptide, was placed under the control of an alcohol oxidase promoter (aox1) in the plasmid ppiczac. the final construction was integrated into the genome of the methylotrophic yeast p. pastoris x33 and the ability to produce xylanase acti ... | 2011 | 21402188 |
| biomimetic conversion of (-)-fusoxypyridone and (-)-oxysporidinone to (-)-sambutoxin: further evidence for the structure of the tricyclic pyridone alkaloid, (-)-fusoxypyridone. | biomimetic-type reactions of the tricyclic pyridone alkaloid, (-)-fusoxypyridone [(-)-4,6'-anhydrooxysporidinone] (1), recently encountered in an endophytic strain of fusarium oxysporum, and (-)-oxysporidinone (2) afforded (-)-sambutoxin (3) and an analogue of 1, identified as (-)-1'(6')-dehydro-4,6'-anhydrooxysporidinone (4), thus confirming the structure previously proposed for 1 and suggesting that 1-3 bear the same relative stereochemistry. oxidation of 4 with 2,3-dichloro-5,6-dicyano-1,4-be ... | 2011 | 21419624 |
| chemical constituents of picea neoveitchii. | four flavonoids, 5,7,4'-trihydroxy-3,8,-dimethoxy-6-c-methylflavone (1), 5,8,4'-trihydroxy-3,7-dimethoxy-6-c-methylflavone (2), 7-methoxy-6-c-methylkaempferol (3) and kaempferol-7-o-(2?-e-p-coumaroyl)-a-l-arabinofuranoside (4), together with 15 known compounds, were isolated from the twigs and leaves of picea neoveitchii mast. their structures were elucidated on the basis of analyses of spectroscopic data. compound 4 showed strong anti-fungal activity against fusarium oxysporum whereas compounds ... | 2011 | 21334699 |
| distinct colonization patterns and cdna-aflp transcriptome profiles in compatible and incompatible interactions between melon and different races of fusarium oxysporum f. sp. melonis. | fusarium oxysporum f. sp. melonis snyd. & hans. (fom) causes fusarium wilt, the most important infectious disease of melon (cucumis melo l.). the four known races of this pathogen can be distinguished only by infection on appropriate cultivars. no molecular tools are available that can discriminate among the races, and the molecular basis of compatibility and disease progression are poorly understood. resistance to races 1 and 2 is controlled by a single dominant gene, whereas only partial polyg ... | 2011 | 21338485 |
| surface-modified sulfur nanoparticles: an effective antifungal agent against aspergillus niger and fusarium oxysporum. | surface-modified sulfur nanoparticles (snps) of two different sizes were prepared via a modified liquid-phase precipitation method, using sodium polysulfide and ammonium polysulfide as starting material and polyethylene glycol-400 (peg-400) as the surface stabilizing agent. surface topology, size distribution, surface modification of snps with peg-400, quantitative analysis for the presence of sulfur in nanoformulations, and thermal stability of snps were determined by atomic force microscopy (a ... | 2011 | 21350853 |
| in planta and soil quantification of fusarium oxysporum f. sp. ciceris and evaluation of fusarium wilt resistance in chickpea with a newly developed quantitative polymerase chain reaction assay. | fusarium wilt of chickpea caused by fusarium oxysporum f. sp. ciceris can be managed by risk assessment and use of resistant cultivars. a reliable method for the detection and quantification of f. oxysporum f. sp. ciceris in soil and chickpea tissues would contribute much to implementation of those disease management strategies. in this study, we developed a real-time quantitative polymerase chain reaction (q-pcr) protocol that allows quantifying f. oxysporum f. sp. ciceris dna down to 1 pg in s ... | 2011 | 21219129 |
| horizontal gene and chromosome transfer in plant pathogenic fungi affecting host range. | plant pathogenic fungi adapt quickly to changing environments including overcoming plant disease resistance genes. this is usually achieved by mutations in single effector genes of the pathogens, enabling them to avoid recognition by the host plant. in addition, horizontal gene transfer (hgt) and horizontal chromosome transfer (hct) provide a means for pathogens to broaden their host range. recently, several reports have appeared in the literature on hgt, hct and hybridization between plant path ... | 2011 | 21223323 |
| antifungal lipopeptides from bacillus amyloliquefaciens strain bo7. | three new lipopeptides (1-3) were isolated from the organic extract of bacillus amyloliquefaciens strain (bo7). these compounds represented the major constituents (>60%) of the total cell lipids extractable with chcl(3)/meoh (2:1). elucidation of their chemical structure was carried out by spectroscopic analyses, including 1d and 2d nmr spectroscopy, mass spectrometry (ms), and secondary ion mass spectrometry (ms/ms), along with chemical degradation. the compounds are members of the surfactins f ... | 2011 | 21235220 |
| a novel antioestrogen agent (3r,6r)-bassiatin inhibits cell proliferation and cell cycle progression by repressing cyclin d1 expression in 17β-oestradiol-treated mcf-7 cells. | bassiatin [(3r,6r)-bassiatin] was isolated from the endogenous fungus fusarium oxysporum j8-1-2. we found that oestrogen-dependent cell growth was inhibited by bassiatin in mcf-7 erα (oestrogen receptor α)-positive breast cancer cells. in addition, the mrna and protein levels of erα and the oestrogen-responsive gene cyclin d1 were down-regulated by bassiatin in the presence of 17β-oestradiol. co-treatment of 17β-oestradiol and bassiatin increased phospho-cyclin d1 (thr286), an indicator of cycli ... | 2011 | 21241249 |
| rapid detection of aspergillus flavus in rice using biofunctionalized carbon nanotube field effect transistors. | in the present study, we have used carbon nanotube field effect transistors (fet) that have been functionalized with protein g and igg to detect aspergillus flavus in contaminated milled rice. the adsorbed protein g on the carbon nanotubes walls enables the igg anti-aspergillus antibodies to be well oriented and therefore to display full antigen binding capacity for fungal antigens. a solution of tween 20 and gelatine was used as an effective blocking agent to prevent the non-specific binding of ... | 2011 | 20632162 |
| interactions between various microbes and ginseng botanicals. | three kinds of interactions occur between ginseng botanicals and microorganisms: a) spoilage of the botanical by various fungi (e.g., aspergillus, penicillium, alternaria, and eurotium species) and bacteria; b) transformation of ginsenosides into more bioactive forms by bacteria such as intrasporangium sp. gs603, microbacterium sp. gs514, caulobacter leidyia, bifidobacterium sp. int57, bifidobacterium sp. sj32, fusobacterium sp. and bacteroides sp., and moulds (e.g., aspergillus niger, fusarium ... | 2011 | 21254831 |
| fusarium oxysporum and its bacterial consortium promote lettuce growth and expansin a5 gene expression through microbial volatile organic compound (mvoc) emission. | fusarium oxysporum msa 35 [wild-type (wt) strain] is a nonpathogenic fusarium strain, which exhibits antagonistic activity to plant pathogenic f. oxysporum isolates. the fungus lives in association with a consortium of ectosymbiotic bacteria. the wt strain, when cured of the bacterial symbionts [the cured (cu) form], is pathogenic, causing wilt symptoms similar to those of pathogenic f. oxysporum f. sp. lactucae. both wt and cu msa 35 strains produce microbial volatile organic compounds (mvocs), ... | 2011 | 21255049 |
| zingipain, a cysteine protease from zingiber ottensii valeton rhizomes with antiproliferative activities against fungi and human malignant cell lines. | the objective of this study was to investigate the activity of a protein identified as cysteine protease, purified from zingiber ottensii valeton rhizomes, in terms of antiproliferation against fungi, bacteria, and human malignant cell lines. by means of buffer extraction followed by (nh(4))(2)so(4) precipitation and ion-exchange chromatography, the obtained dominant protein (designated f50) was submitted to non-denaturing and reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (s ... | 2011 | 21442550 |
| monitoring of pathogenic and non-pathogenic fusarium oxysporum strains during tomato plant infection. | monitoring of pathogenic strains of fusarium oxysporum (fox), which cause wilt and rots on agricultural and ornamental plants, is important for predicting disease outbreaks. since both pathogenic and non-pathogenic strains of fox are ubiquitous and are able to colonize plant roots, detection of fox dna in plant material is not the ultimate proof of an ongoing infection which would cause damage to the plant. we followed the colonization of tomato plants by strains fox f. sp. radicis-lycopersici z ... | 2011 | 21255375 |
| reverse transcription of the pfoxc mitochondrial retroplasmids of fusarium oxysporum is protein primed. | 2011 | 21255388 | |
| current status of the taxonomic position of fusarium oxysporum formae specialis cubense within the fusarium oxysporum complex. | fusarium oxysporum is an asexual fungal species that includes human and animal pathogens and a diverse range of nonpathogens. pathogenic and nonpathogenic strains of this species can be distinguished from each other with pathogenicity tests, but not with morphological analysis or sexual compatibility studies. substantial genetic diversity among isolates has led to the realization that f. oxysporum represents a complex of cryptic species. f. oxysporum f. sp cubense (foc), causal agent of fusarium ... | 2011 | 21256980 |
| distinction of fusarium oxysporum fungal isolates (strains) using ftir-atr spectroscopy and advanced statistical methods. | fusarium is a large fungi genus of a large variety of species and strains which inhabits soil and vegetation. it is distributed worldwide and affiliated to both warm and cold weather. fusarium oxysporum species, for instance, cause the fusarium wilt disease of plants, which appears as a leaf wilting, yellowing and eventually plant death. early detection and identification of these pathogens are very important and might be critical for their control. previously, we have managed to differentiate a ... | 2011 | 21258677 |
| loss of camp-dependent protein kinase a affects multiple traits important for root pathogenesis by fusarium oxysporum. | the soilborne fungal pathogen fusarium oxysporum causes vascular wilt and root rot diseases in many plant species. we investigated the role of cyclic amp-dependent protein kinase a of f. oxysporum (focpka) in growth, morphology, and root attachment, penetration, and pathogenesis in arabidopsis thaliana. affinity of spore attachment to root surfaces of a. thaliana, observed microscopically and measured by atomic force microscopy, was reduced by a loss-of-function mutation in the gene encoding the ... | 2011 | 21261464 |
| synthesis and anti-microbial evaluation of some novel 1,2,4-triazole derivatives. | triazoles with different substituent groups are found to possess diverse applications in the field of medicine and industry. a series of 4-(substituted ethanoyl)amino-3-mercapto-5-(4-nitro)phenyl-1,2,4-triazoles (nu-1 to nu-15) were synthesized as novel antimicrobial agents starting from 4-nitrobenzoic acid. the chemical structures of these newly synthesized compounds were elucidated by ir, 1h nmr, 13c nmr, fab+ -ms spectral data and elemental analysis. their antimicrobial activities against sta ... | 2011 | 21485294 |
| predicting methyl iodide emission, soil concentration, and pest control in a two-dimensional chamber system. | due to ever-increasing state and federal regulations, the future use of fumigants is predicted on reducing negative environmental impacts while offering sufficient pestcontrol efficacy. to foster the development of a best management practice, an integrated tool is needed to simultaneously predict fumigant movement and pest control without having to conduct elaborate and costly experiments. the objective of this study was (i) to present a two-dimensional (2-d) mathematical model to describe both ... | 2011 | 21488499 |
| synthesis and antimicrobial activity of some new 4-hetarylpyrazole and furo[2,3-c]pyrazole derivatives. | in continuation of our efforts to find a new class of antimicrobial agents, a series of 4-hetarylpyrazoles and furo[2,3-c]pyrazoles were prepared via the reaction of 2-chloro-1-(5-hydroxy-3-methyl-1-phenyl-1h-pyrazol-4-yl)ethanone (1) with an appropriate nucleophilic reagents. these compounds were screened for their antibacterial activity against gram-positive bacteria (bacillus subtilis and bacillus thuringiensis), gram-negative bacteria (escherichia coli and pseudomonas aeruginosa) and antifun ... | 2011 | 21489661 |
| auxin signaling and transport promote susceptibility to the root-infecting fungal pathogen fusarium oxysporum in arabidopsis. | fusarium oxysporum is a root-infecting fungal pathogen that causes wilt disease on a broad range of plant species, including the model plant arabidopsis thaliana. currently, very little is known about the molecular or physiological processes that are activated in the host during infection and the roles these processes play in resistance and susceptibility to f. oxysporum. in this study, we analyzed global gene expression profiles of f. oxysporum-infected arabidopsis plants. genes involved in jas ... | 2011 | 21281113 |
| development of disease-resistant marker-free tomato by r/rs site-specific recombination. | the selection marker genes, imparting antibiotic or herbicide resistance, in the final transgenics have been criticized by the public and considered a hindrance in their commercialization. multi-auto-transformation (mat) vector system has been one of the strategies to produce marker-free transgenic plants without using selective chemicals and plant growth regulators (pgrs). in the study reported here, isopentenyltransferase (ipt) gene was used as a selection marker and wasabi defensin (wd) gene, ... | 2011 | 21293863 |
| chemical constituents from endophytic fungus fusarium oxysporum. | a new oxysporidinone analogue (1) and a new 3-hydroxyl-2-piperidinone derivative (2), along with the known compounds (-)-4,6'-anhydrooxysporidinone (3), (+)-fusarinolic acid (4), gibepyrone d (5), beauvercin (6),cerevisterol (7), fusaruside (8), and (2s,2'r,3r,3'e,4e,8e)-1-o-d-glucopyranosyl-2-n-(2'-hydroxy-3'-octadecenoyl)-3-hydroxy-9-methyl-4,8-sphingadienine (9) were isolated from fusarium oxysporum. compounds 1-9 were evaluated for cytotoxicity using the mtt method against cancer cell lines, ... | 2011 | 21497643 |
| evaluations of shorter exposures of contact lens cleaning solutions against fusarium oxysporum species complex and fusarium solani species complex to simulate inappropriate usage. | an outbreak of fusarium keratitis in contact lens users resulted in withdrawal of renu with moistureloc solution, although the exact cause of the outbreak remains enigmatic. we evaluated current and discontinued multipurpose cleaning solutions (mpss; moistureloc, equate, multiplus, and optifree express) against plankton- and biofilm-derived cells of fusarium oxysporum species complex (fosc) and f. solani species complex (fssc). the methods included a traditional assay based on cfu counts and a n ... | 2011 | 21300826 |
| an antifungal peptide with antiproliferative activity toward tumor cells from red kidney beans. | a 7.3-kda antifungal peptide was purified from dried red kidney beans. the purification procedure entailed ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on cm-cellulose, followed by fast protein liquid chromatography-gel filtration on superdex 75. the peptide was unadsorbed on deae-cellulose but adsorbed on affi-gel blue gel and cm-cellulose. it exhibited a molecular mass of 7.3 kda in gel filtration and also in sds-polya ... | 2011 | 21309741 |
| disease control effect of strevertenes produced by streptomyces psammoticus against tomato fusarium wilt. | during screening of microorganisms producing antifungal metabolites, streptomyces psammoticus strain kp1404 was isolated. the culture extract of this strain showed potent disease control efficacy against fusarium wilt on tomato plants. the antifungal metabolites st-1 and st-2 were isolated from the culture extract using a variety of chromatographic procedures. on the basis of ms and nmr spectrometric analysis, the structures of the antifungal active compounds st-1 and st-2 were determined to be ... | 2011 | 21314121 |
| asymmetric total synthesis of 6-tuliposide b and its biological activities against tulip pathogenic fungi. | the structure-activity relationship was investigated to evaluate the antifungal activities of tuliposides and tulipalins against tulip pathogenic fungi. 6-tuliposide b was effectively synthesized via the asymmetric baylis-hillman reaction. tuliposides and tulipalins showed antifungal activities against most of the strains tested at high concentrations (2.5 mm), while botrytis tulipae was resistant to tuliposides. tulipalin formation was involved in the antifungal activity, tulipalin a showed hig ... | 2011 | 21512240 |
| a novel antifungal peptide from foxtail millet seeds. | antifungal proteins (afp) help plants to combat phytopathogenic fungi and thus protect plants from the devastating damage caused by fungal infections and prevent massive economic losses. to date, several proteins with antibacterial and/or antifungal properties have been isolated and characterized from different plant species and tissues; however, there are no reports concerning the antifungal peptide from foxtail millet seeds. | 2011 | 21445868 |
| steroids and an oxylipin from an algicolous isolate of aspergillus flavus. | a new oxylipin, (8e,12z)-10,11-dihydroxyoctadeca-8,12-dienoic acid (1), a new steroid, 3ß,4a-dihydroxy-26-methoxyergosta-7,24(28)-dien-6-one (2), and four known steroids, episterol (3), (22e,24r)-ergosta-7,22-dien-3ß,5a,6a-triol (4), (22e,24r)-ergosta-5,22-dien-3ß-ol (5), and (22e,24r)-ergosta-4,6,8(14),22-tetraen-3-one (6), were isolated from the cultures of aspergillus flavus, an endophytic fungus isolated from the marine red alga corallina officinalis. their structures and relative stereochem ... | 2011 | 21452344 |
| glutathione reductase/glutathione is responsible for cytotoxic elemental sulfur tolerance via polysulfide shuttle in fungi. | fungi that can reduce elemental sulfur to sulfide are widely distributed, but the mechanism and physiological significance of the reaction have been poorly characterized. here, we purified elemental sulfur-reductase (sr) and cloned its gene from the elemental sulfur-reducing fungus fusarium oxysporum. we found that nadph-glutathione reductase (gr) reduces elemental sulfur via glutathione as an intermediate. a loss-of-function mutant of the sr/gr gene generated less sulfide from elemental sulfur ... | 2011 | 21474441 |