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molecular evidence for the involvement of a polygalacturonase-inhibiting protein, ghpgip1, in enhanced resistance to verticillium and fusarium wilts in cotton.polygalacturonase-inhibiting protein (pgip), belonging to a group of plant defence proteins, specifically inhibits endopolygalacturonases secreted by pathogens. herein, we showed that purified ghpgip1 is a functional inhibitor of verticillium dahliae and fusarium oxysporum f. sp. vasinfectum, the two fungal pathogens causing cotton wilt. transcription of ghpgip1 was increased in cotton upon infection, wounding, and treatment with defence hormone and h2o2. resistance by ghpgip1 was examined by it ...201728079053
enzyme inhibitory metabolites from endophytic penicillium citrinum isolated from boswellia sacra.fungal endophytes establish an important niche within the host plant through the secretion of chemical constituents. isolation of bioactive metabolites could be a vital source for inhibiting the function of enzymes such as α-glucosidase and urease. the present study aimed to elucidate the potential of endophytes associated with boswellia sacra through bioassay-guided isolation and identification of secondary metabolites with enzyme inhibitory ability. endophytic fungal strains viz. penicillium c ...201728220202
biocontrol potential of halotolerant bacterial chitinase from high yielding novel bacillus pumilus mcb-7 autochthonous to mangrove ecosystem.the multifaceted role of chitinase in medicine, agriculture, environmental remediation and various other industries greatly demands the isolation of high yielding chitinase producing microorganisms with improved properties. the current study aimed to investigate the isolation, characterization and biocontrol prospective of chitinase producing bacterial strains autochthonous to the extreme conditions of mangrove ecosystems. among the 51 bacterial isolates screened, bacillus pumilus mcb-7 with hig ...201728364802
streptomyces luozhongensis sp. nov., a novel actinomycete with antifungal activity and antibacterial activity.a novel actinomycete strain, designated trm 49605(t), was isolated from a desert soil sample from lop nur, xinjiang, north-west china, and characterised using a polyphasic taxonomic approach. the strain exhibited antifungal activity against the following strains: saccharomyces cerevisiae, curvularia lunata, aspergillus flavus, aspergillus niger, fusarium oxysporum, penicillium citrinum, candida albicans and candida tropicalis; antibacterial activity against bacillus subtilis, staphylococcus epid ...201727752797
diversity, phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with monarda citriodora l.present study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of the monarda citriodora cerv. ex lag. (lamiaceae/labiatae). anticancer and antimicrobial potential of isolated endophytes have also been investigated.201728264654
identification of root-secreted compounds involved in the communication between cucumber, the beneficial bacillus amyloliquefaciens, and the soil-borne pathogen fusarium oxysporum.colonization of plant growth-promoting rhizobacteria (pgpr) is critical for exerting their beneficial effects on the plant. root exudation is a major factor influencing the colonization of both pgpr and soil-borne pathogens within the root system. however, the tripartite interaction of pgpr, plant roots, and soil-borne pathogens is poorly understood. we screened root exudates for signals that mediate tripartite interactions in the rhizosphere. in a split-root system, we found that root colonizat ...201727937752
phytotoxic, antifungal and immunosuppressive metabolites from aspergillus terreus qt122 isolated from the gut of dragonfly.insect gut microbes have been considered as a resource for bioactive metabolites. the aim of this study was to characterize the compounds of a fungus aspergillus terreus qt122 associated with the gut of dragonfly. five main phytotoxic, antifungal, and immunosuppressive substances were isolated from the fungus qt122. the structures of such compounds were identified as emodin (1), 1-methyl emodin (2), terrein (3), methyl 6-acetyl-4-methoxy-7,8-dihydroxynaphthalene-2-carboxylate (4), and dihydrogeo ...201727844127
plant growth-promoting endophytic bacteria versus pathogenic infections: an example of bacillus amyloliquefaciens rwl-1 and fusarium oxysporum f. sp. lycopersici in tomato.fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic bacillus amyloliquefaciens rwl-1 against pathogenic fusarium oxysporum f. sp. lycopersici. the results revealed significant suppressio ...201728321368
trans-cinnamic acid and xenorhabdus szentirmaii metabolites synergize the potency of some commercial fungicides.development of novel approaches for the control of fungal phytopathogens is desirable. in this study we hypothesized that the combination of commercial fungicides with certain enhancing agents could result in synergistic levels of control. prior research has indicated that trans-cinnamic-acid (tca), a metabolite of the bacteria photorhabdus luminescens and metabolites of xenorhabdus szentirmaii are particularly toxic to various phytpathogenic fungi when compared to metabolites of other xenorhabd ...201728322849
improved catalytic and antifungal activities of bacillus thuringiensis cells with surface display of chi9602δsp.the cell-surface display of chitinase chi9602δsp was constructed on bacillus thuringiensis bmb171 by using two repeat n-terminal regions of autolysin (mbgn)2 as the anchoring motif. this study aimed to prepare a chitinolytic whole-cell b. thuringiensis biocatalyst with improved catalytic and antifungal activities.201727782360
enhancement of seed dehiscence by seed treatment with talaromyces flavus gg01 and gg04 in ginseng (panax ginseng).seed dehiscence of ginseng (panax ginseng c. a. mayer) is affected by moisture, temperature, storage conditions and microbes. several microbes were isolated from completely dehisced seed coat of ginseng cultivars, chunpoong and younpoong at gumsan, korea. we investigated the potential of five talaromyces flavus isolates from the dehiscence of ginseng seed in four traditional stratification facilities. the isolates showed antagonistic activities against fungal plant pathogens, such as cylindrocar ...201728167883
a new depigmenting-antifungal methylated grindelane from grindelia chiloensis.the new methylated grindelane diterpenoid, 7β-hydroxy-8(17)-dehydrogrindelic acid (1b), together with the known 7α-hydroxy-8(17)-dehydrogrindelic acid (2a), 6-oxogrindelic acid (3a), 4β-hydroxy-6-oxo-19-norgrindelic (4a), 19-hydroxygrindelic acid (5a), 18-hydroxygrindelic acid (6a), 4α-carboxygrindelic acid (7a), 17-hydroxygrindelic acid (8a), 6α-hydroxygrindelic acid (9a), 8,17-bisnor-8-oxagrindelic acid (10a), 7α,8α-epoxygrindelic acid (11a), and strictanonic acid (12a) as methyl esters were o ...201728135782
antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa.plants harbor endophytes with potential bioactivity. markhamia tomentosa (benth) k. schum ex. engl. (bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities.201727937112
disseminated phaeohyphomycosis in a dog.phaeohyphomycosis is a rare but emerging disease caused by dematiaceous fungi. here we describe the case of an immunosuppressed dog with disseminated phaeohyphomycosis secondary to bipolaris spicifera infection. regionally extensive infiltration of the paw pads, skin, myocardium, liver, renal interstitium and diaphragm was identified on histopathology. candida glabrata and fusarium oxysporum were also cultured from multiple sites post-mortem. the dog was treated with fluconazole, itraconazole, t ...201728275534
prospecting fungal parasites of the potato cyst nematode globodera pallida using a rapid screening technique.seven filamentous fungal species were isolated from individual eggs of globodera pallida cysts collected from infested fields in shelley idaho, usa and identified as chaetomium globosum, fusarium oxysporum, fusarium solani, fusarium tricinctum, microdochium bolleyi, purpureocillium lilacinum, and plectosphaerella cucumerina. their ability to reduce infection by g. pallida in planta were assessed in simple, reproducible micro-rhizosphere chambers (micro-rocs). all fungi reduced g. pallida infecti ...201728375550
seed vectored endophytic bacteria modulate development of rice seedlings.the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens.201728375579
application and bioactive properties of cati, a trypsin inhibitor from capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells.during the last few years, a growing number of antimicrobial peptides have been isolated from plants and particularly from seeds. recent results from our laboratory have shown the purification of a new trypsin inhibitor, named cati, from chilli pepper (capsicum annuum l.) seeds. this study aims to evaluate the antifungal activity and mechanism of action of cati on phytopathogenic fungi and detect the presence of protease inhibitors in other species of this genus.201728139827
recombinant exochitinase of the thermophilic mould myceliopthora thermophila bja: characteristics and utility in generating n-acetyl glucosamine and in biocontrol of phytopathogenic fungi.chitinase from the thermophilic mould myceliopthora thermophila bja (mtchit) is an acid tolerant, thermostable and organic solvent stable biocatalyst which does not require any metal ions for its activity. to produce high enzyme titres, reduce fermentation time and overcome the need for induction, this enzyme has been heterologously expressed under gap promoter in the gras yeast, pichia pastoris. the production medium supplemented with the permeabilizing agent tween-20 supported two-fold higher ...201727689686
elucidation of biocontrol mechanisms of trichoderma harzianum against different plant fungal pathogens: universal yet host specific response.in the present study, different transcripts of trichoderma harzianum thhp-3 were evaluated for their response against four fungal pathogens fusarium oxysporum, colletotrichum capsici, colletotrichum truncatum and gloesercospora sorghi using rt-qpcr. the time course study of t. harzianum transcripts related to signal transduction, lytic enzymes, secondary metabolites and various transporters revealed variation in expression against four fungal pathogens. in a broader term, the transcripts were up ...201727856319
[fusarium species associated with basal rot of garlic in north central mexico and its pathogenicity].garlic in mexico is one of the most profitable vegetable crops, grown in almost 5,451ha; out of which more than 83% are located in zacatecas, guanajuato, sonora, puebla, baja california and aguascalientes. blossom-end rot caused by fusarium spp is widely distributed worldwide and has been a limiting factor in onion and garlic production regions, not only in mexico but also in other countries. the presence of fusarium oxysporum has been reported in guanajuato and aguascalientes. fusarium culmorum ...201727590608
comparison of dna microarray, loop-mediated isothermal amplification (lamp) and real-time pcr with dna sequencing for identification of fusarium spp. obtained from patients with hematologic malignancies.the performance of three molecular biology techniques, i.e., dna microarray, loop-mediated isothermal amplification (lamp), and real-time pcr were compared with dna sequencing for properly identification of 20 isolates of fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. dna microarray, lamp and real-time pcr identified 16 (80%) out of 20 samples as fusarium solani species complex (fssc) and four (20%) as fusarium spp. t ...201728324245
multiple garlic (allium sativum l.) micrornas regulate the immunity against the basal rot fungus fusarium oxysporum f. sp. cepae.the basal plate rot fungus, fusarium oxysporum f. sp. cepae (foc), is the most devastating pathogen posing a serious threat to garlic (allium sativum l.) production worldwide. micrornas (mirnas) are key modulators of gene expression related to development and defense responses in eukaryotes. however, the mirna species associated with garlic immunity against foc are yet to be explored. in the present study, a small rna library developed from foc infected resistant garlic line was sequenced to ide ...201728224922
antimicrobial activity of medicinal plants correlates with the proportion of antagonistic endophytes.medicinal plants are known to harbor potential endophytic microbes, due to their bioactive compounds. in a first study of ongoing research, endophytic bacteria were isolated from two medicinal plants, hypericum perforatum and ziziphora capitata with contrasting antimicrobial activities from the chatkal biosphere reserve of uzbekistan, and their plant-specific traits involved in biocontrol and plant growth promotion were evaluated. plant extracts of h. perforatum exhibited a remarkable activity a ...201728232827
medium ph in submerged cultivation modulates differences in the intracellular protein profile of fusarium oxysporum.fusarium oxysporum is a filamentous fungus that damages a wide range of plants and thus causes severe crop losses. in fungal pathogens, the genes and proteins involved in virulence are known to be controlled by environmental ph. here, we report the influence of culture-medium ph (5, 6, 7, and 8) on the production of degradative enzymes involved in the pathogenesis of f. oxysporum urm 7401 and on the 2d-electrophoresis profile of intracellular proteins in this fungus. f. oxysporum urm 7401 was gr ...201728281888
antifungal activity, yield, and composition of ocimum gratissimum essential oil.ocimum gratissimum l. or clove basil, belongs to the lamiaceae family, has various desirable uses and applications. beyond its aromatic, seasoning, and medicinal applications, this plant also has antimicrobial activity. this study was aimed at assessing the antifungal activity, yield, and composition of the essential oil (eo) of o. gratissimum. the species was cultivated in garden beds with dystrophic red latosol soil type containing high organic-matter content. the eo was obtained by hydrodisti ...201728362991
members of gammaproteobacteria as indicator species of healthy banana plants on fusarium wilt-infested fields in central america.culminating in the 1950's, bananas, the world's most extensive perennial monoculture, suffered one of the most devastating disease epidemics in history. in latin america and the caribbean, fusarium wilt (fw) caused by the soil-borne fungus fusarium oxysporum f. sp. cubense (foc), forced the abandonment of the gros michel-based export banana industry. comparative microbiome analyses performed between healthy and diseased gros michel plants on fw-infested farms in nicaragua and costa rica revealed ...201728345666
suppression of fusarium wilt of cucumber by ammonia gas fumigation via reduction of fusarium population in the field.cucumber plants subjected to consecutive monoculture for 9 years were found to suffer from severe fusarium wilt disease, caused by the soil-borne fungus fusarium oxysporum f. sp. cucumerinum j. h. owen. in the present study, greenhouse experiments were performed to evaluate the influence of ammonia gas fumigation on fusarium wilt suppression, fungal abundance and fungal community composition. results showed that ammonia gas fumigation remarkably reduced disease incidence from 80% to 27%, resulti ...201728230182
concurrent methylation and demethylation of arsenic by fungi and their differential expression in the protoplasm proteome.microbial methylation and demethylation are central to arsenic's (as) biogeochemical cycling. here, the transformations of monomethylarsonic acid (mma(v)) (50 mg l(-1)) for 15 days in cells of as-methylating fungi, fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1, were evaluated, and trace concentrations of as(iii) and as(v) were observed in fungal cell extracts. trace amounts of dma(v) were also detected in mma(v) and p. janthinellum sm-12f4 incuba ...201728336093
effector gene suites in some soil isolates of fusarium oxysporum are not sufficient predictors of vascular wilt in tomato.seventy-four f. oxysporum soil isolates were assayed for known effector genes present in an f. oxysporum f.sp. lycopersici race 3 tomato wilt strain (fol mn-25) obtained from the same fields in manatee county, florida. based on the presence or absence of these genes, four haplotypes were defined, two of which represented 96% of the surveyed isolates. these two most common effector haplotypes contained either all or none of the assayed race 3 effector genes. we hypothesized that soil isolates wit ...201728323535
nitrate increased cucumber tolerance to fusarium wilt by regulating fungal toxin production and distribution.cucumber fusarium wilt, induced by fusarium oxysporum f. sp. cucumerinum (foc), causes severe losses in cucumber yield and quality. nitrogen (n), as the most important mineral nutrient for plants, plays a critical role in plant-pathogen interactions. hydroponic assays were conducted to investigate the effects of different n forms (nh₄⁺ vs. no₃(‒)) and supply levels (low, 1 mm; high, 5 mm) on cucumber fusarium wilt. the no₃(‒)-fed cucumber plants were more tolerant to fusarium wilt compared with ...201728287458
the plasma membrane-localized sucrose transporter ibsweet10 contributes to the resistance of sweet potato to fusarium oxysporum.sweet (sugars will eventually be exported transporter) proteins, a novel family of sugar transporters, mediate the diffusion of sugars across cell membranes and acts as key players in sucrose phloem loading. manipulation of sweet genes in plants leads to various effects on resistance to biotic and abiotic stresses due to disruption of sugar efflux and changes in sugar distribution. in this study, a member of the sweet gene family, ibsweet10, was cloned from the sweet potato line nd98. mrna expre ...201728261250
response of atnpr1-expressing cotton plants to fusarium oxysporum f. sp. vasinfectum isolates.in our earlier investigation, we had demonstrated that transgenic cotton plants expressing atnpr1 showed significant tolerance to fusarium oxysporum f. sp. vasinfectum, isolate 11 (fov11) and several other pathogens. the current study was designed to further characterize the nature of the protection provided by atnpr1 expression and its limitations. green fluorescent protein-expressing fov11 was generated and used to study the progression of the disease within the plant. the results show that th ...201728250590
down-regulatory effect of thymus vulgaris l. on growth and tri4 gene expression in fusarium oxysporum strains.the aims of this study were to evaluate the efficacy of thymus vulgaris (t. vulgaris) essential oil on the fungal growth and tri4 gene expression in fusarium oxysporum (f. oxysporum) strains. the oil was obtained by water-distillation using a clevenger-type system. the chemical composition of the essential oil was obtained by gas chromatography- mass spectroscopy (gc-ms) and by retention indices. the antifungal activity was evaluated by broth microdilution assay. a quantitative real time rt-pcr ...201728062283
expression and distribution of extensins and agps in susceptible and resistant banana cultivars in response to wounding and fusarium oxysporum.banana fusarium wilt caused by fusarium oxysporum f. sp. cubense (foc) is soil-borne disease of banana (musa spp.) causing significant economic losses. extensins and arabinogalactan proteins (agps) are cell wall components important for pathogen defence. their significance for foc resistance in banana was not reported so far. in this study, two banana cultivars differing in foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and agps aft ...201728218299
involvement of salicylic acid, ethylene and jasmonate acid signaling pathways in susceptibility of tomato for fusarium oxysporum.phytohormones such as salicylic acid (sa), ethylene (et) and jasmonic acid (ja) play key roles in plant defense following pathogen attack. involvement of these hormones in susceptibility following fusarium oxysporum (fo) infection has mostly been studied in arabidopsis thaliana. fo, however, causes vascular wilt disease in a broad range of crops, including tomato (solanum lycopersicum). surprisingly little is known about the involvement of these phytohormones in susceptibility of tomato towards ...201728390170
rapd assisted selection of black gram (vigna mungo l. hepper) towards the development of multiple disease resistant germplasm.black gram (vigna mungo l. hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. the yield potential of black gram is shallow due to lack of genetic variability and biotic stress susceptibility. core biotic stress factors include mung bean yellow mosaic virus (mymv), urdbean leaf crinkle virus (uclv), wilt (fusarium oxysporum) and powdery mildew (erysiphe polygoni dc). although many studies determine resistant varieties to a parti ...201728389895
rapid and sensitive diagnoses of dry root rot pathogen of chickpea (rhizoctonia bataticola (taub.) butler) using loop-mediated isothermal amplification assay.dry root rot (drr) caused by the fungus rhizoctonia bataticola (taub.) butler, is an emerging disease in chickpea. the disease is often mistaken with other root rots like fusarium wilt, collar rot and black root rot in chickpea. therefore, its timely and specific detection is important. current detection protocols are either based on mycological methods or on protocols involving dna amplification by polymerase chain reaction (pcr). here we report the rapid and specific detection of r. bataticola ...201728218268
investigation of genetic diversity of fusarium oxysporum f. sp. fragariae using pcr-rflp.fusarium wilts of strawberry, caused by fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. reliable chemical control of strawberry fusarium wilt disease is not yet available. moreover, other well-known f. oxysporum have different genetic information from f. oxysporum f. sp. fragariae. this analysis investigates the genetic diversity of strawberry fusairu ...201728381961
fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems.the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ...201728369915
transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.fusarium oxysporum f. sp. melonis snyd. & hans race 1.2 (fom1.2) is the most virulent and yield-limiting pathogen of melon (cucumis melo l.) worldwide. current information suggest that the resistance to race 1.2 is controlled by multiple recessive genes and strongly affected by the environment. rna-seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes deployed during melon-fom1.2 interaction in the resistant doubled haploid line nad and in the ...201728367157
draft genome sequence of an isolate of fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt of eggplant.here, we present the genome sequence of an isolate (14004) of fusarium oxysporum f. sp. melongenae, an eggplant pathogen. the final assembly consists of 1,631 scaffolds with 53,986,354 bp (g+c content, 46.4%) and 16,485 predicted genes.201728209821
paenibacillus polymyxa nsy50 suppresses fusarium wilt in cucumbers by regulating the rhizospheric microbial community.paenibacillus polymyxa (p. polymyxa) nsy50, isolated from vinegar residue substrate, suppresses the growth of fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. the aim of the present study was to evaluate the effects of nsy50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to fusarium oxysporum. bacterial and fungal communities were investigated by illumina sequencing of t ...201728198807
potential of burkholderia seminalis tc3.4.2r3 as biocontrol agent against fusarium oxysporum evaluated by mass spectrometry imaging.species of genus burkholderia display different interaction profiles in the environment, causing either several diseases in plants and animals or being beneficial to some plants, promoting their growth, and suppressing phytopathogens. burkholderia spp. also produce many types of biomolecules with antimicrobial activity, which may be commercially used to protect crops of economic interest, mainly against fungal diseases. herein we have applied matrix-assisted laser desorption/ionization mass spec ...201728194740
development of a hydrolysis probe-based real-time assay for the detection of tropical strains of fusarium oxysporum f. sp. cubense race 4.fusarium oxysporum f. sp. cubense (foc) is one of the most important threats to global banana production. strategies to control the pathogen are lacking, with plant resistance offering the only long-term solution, if sources of resistance are available. prevention of introduction of foc into disease-free areas thus remains a key strategy to continue sustainable banana production. in recent years, strains of foc affecting cavendish bananas have destroyed plantations in a number of countries in as ...201728178348
a rare case of subcutaneous fusariosis in an immunocompetent patient.fusarium species can cause diseases in immunocompromised patients, whereas have rarely been reported as pathogens in immunocompetent individuals. fusarium oxysporum and f. solani species complex are the most frequent pathogenic species. primary subcutaneous hyalohyphomycosis due to these filamentous fungi is an extremely rare pathology, especially in immunocompetent patients. only 37 cases of deep fusarium infection in healthy patient have been reported in international literature. we report a c ...201728166614
small rnas regulate the biocontrol property of fluorescent pseudomonas strain psd.the production of biocontrol factors by pseudomonads is reported to be controlled at the post-transcriptional level by the gacs/gaca signal transduction pathway. this involves rna-binding translational repressor proteins, rsma and rsme, and the small regulatory rnas (srnas) rsmx, rsmy, and rsmz. while the former represses genes involved in secondary metabolite production, the latter relieves this repression at the end of exponential growth. we have studied the fluorescent pseudomonas strain psd, ...201728164793
development of mesorhizobium ciceri-based biofilms and analyses of their antifungal and plant growth promoting activity in chickpea challenged by fusarium wilt.biofilmed biofertilizers have emerged as a new improved inoculant technology to provide efficient nutrient and pest management and sustain soil fertility. in this investigation, development of a trichoderma viride-mesorhizobium ciceri biofilmed inoculant was undertaken, which we hypothesized, would possess more effective biological nitrogen fixing ability and plant growth promoting properties. as a novel attempt, we selected mesorhizobium ciceri spp. with good antifungal attributes with the assu ...201728148979
trichoderma harzianum containing 1-aminocyclopropane-1-carboxylate deaminase and chitinase improved growth and diminished adverse effect caused by fusarium oxysporum in soybean.an isolate, named trichoderma harzianum t-soybean, showed growth-promoting for soybean seedlings and induced resistance to fusarium oxysporum under greenhouse. compared to control soybean seedlings, fresh weight, dry weight, lateral root number, chlorophyll content, root activity and soluble protein of plants pretreated with t-soybean increased, but initial pod height reduced. furthermore, we found that t-soybean inhibited the growth of f. oxysporum by parasitic function. in addition, plate test ...201728135657
synthesis and antifungal activity of novel myrtenal-based 4-methyl-1,2,4-triazole-thioethers.a series of novel myrtenal derivatives bearing 1,2,4-triazole moiety were designed and synthesized by multi-step reactions in an attempt to develop potent antifungal agents. their structures were confirmed by using uv-vis, ftir, nmr, and esi-ms analysis. antifungal activity of the target compounds was preliminarily evaluated by the in vitro method against fusarium oxysporum f. sp. cucumerinum, physalospora piricola, alternaria solani, cercospora arachidicola, and gibberella zeae at 50 µg/ml. com ...201728125042
discovery of fungal denitrification inhibitors by targeting copper nitrite reductase from fusarium oxysporum.the efficient application of nitrogenous fertilizers is urgently required, as their excessive and inefficient use is causing substantial economic loss and environmental pollution. a significant amount of applied nitrogen in agricultural soils is lost as nitrous oxide (n2o) in the environment due to the microbial denitrification process. the widely distributed fungus fusarium oxysporum is a major denitrifier in agricultural soils and its denitrification activity could be targeted to reduce nitrog ...201728117584
novel 1,2,3-triazolium-functionalized starch derivatives: synthesis, characterization, and evaluation of antifungal property.four novel 1,2,3-triazolium-functionalized starch derivatives were synthesized by n-alkylating the precursor starch derivatives with 1,2,3-triazole with iodomethane based on cuprous-catalyzed azide-alkyne cycloaddition (cuaac). the detailed structural characterization was investigated by means of ftir, uv-vis, (1)h nmr, and (13)c nmr spectra. the antifungal activities of starch derivatives against colletotrichum lagenarium, fusarium oxysporum, and watermelon fusarium, were then assayed by hypha ...201728115090
a tomatinase-like enzyme acts as a virulence factor in the wheat pathogen fusarium graminearum.during their interactions with plants, fungal pathogens employ large numbers of pathogenesis-associated molecules including secreted effectors and enzymes that can degrade various defence compounds. however, in many cases, in planta targets of pathogen-produced enzymes remain unknown. we identified a gene in the wheat pathogen fusarium graminearum, encoding a putative enzyme that shows 84% sequence identity to fotom1, a tomatinase produced by the tomato pathogen fusarium oxysporum f. sp. lycoper ...201728109774
structure-activity relationship of α mating pheromone from the fungal pathogen fusarium oxysporum.during sexual development ascomycete fungi produce two types of peptide pheromones termed a and α. the α pheromone from the budding yeast saccharomyces cerevisiae, a 13-residue peptide that elicits cell cycle arrest and chemotropic growth, has served as paradigm for the interaction of small peptides with their cognate g protein-coupled receptors. however, no structural information is currently available for α pheromones from filamentous ascomycetes, which are significantly shorter and share almo ...201728100777
fusaric acid contributes to virulence of fusarium oxysporum on plant and mammalian hosts.fusaric acid (fa) is amongst the oldest identified secondary metabolites produced by fusarium species, known for a long time to display strong phytotoxicity and moderate toxicity to animal cells; however, the cellular targets of fa and its function in fungal pathogenicity remain unknown. here, we investigated the role of fa in fusarium oxysporum, a soil-borne cross-kingdom pathogen that causes vascular wilt on more than 100 plant species and opportunistic infections in humans. targeted deletion ...201728093838
tricalcium phosphate solubilization and nitrogen fixation by newly isolated aneurinibacillus aneurinilyticus ckmv1 from rhizosphere of valeriana jatamansi and its growth promotional effect.aneurinibacillus aneurinilyticus strain ckmv1 was isolated from rhizosphere of valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260mg/l), nitrogen fixation (202.91nmolethyleneml(-1)h(-1)), indole-3-acetic acid (iaa) (8.1μg/ml), siderophores (61.60%), hcn (hydrogen cyanide) production and antifungal activity. we investigated the ability of isolate ckmv1 to solubilize insoluble p via mechanism of organic acid production. high-per ...201728063921
chemical profile and defensive function of the latex of euphorbia peplus.plant latex is an endogenous fluid secreted from highly specialized laticifer cells and has been suggested to act as a plant defense system. the chemical profile of the latex of euphorbia peplus was investigated. a total of 13 terpenoids including two previously unknown diterpenoids, (2s*,3s*,4r*,5r*,6r*,8r*,l1r*,13s*,14s*,15r*, 16r*)-5,8,15-triacetoxy-3-benzoyloxy-11,16-dihydroxy-9-oxopepluane and (2r*,3r*, 4s*,5r*,7s*,8s*,9s*,l3s*,14s*,15r*)-2,5,8,9,14-pentaacetoxy-3-benzoyloxy-15-hydroxy-7-is ...201728062071
a novel peroxidase purified from marsdenia megalantha latex inhibits phytopathogenic fungi mediated by cell membrane permeabilization.an antifungal class iii peroxidase was purified from marsdenia megalantha latex (named mo-pox) using deae-cellulose and gel filtration chromatography on a superose 12 hr 10/30 column. mm-pox has an apparent molecular mass of 67.0kda and a pi of 5.2, shares identity with other peroxidases, and follows michaelis-menten kinetics. it has a high affinity for guaiacol and hydrogen peroxide. the ph and temperature optima for mm-pox were 5.0-7.0 and 60°c, respectively. the catalytic activity of mm-pox w ...201728057569
bioactive secondary metabolites with multiple activities from a fungal endophyte.in order to replace particularly biohazardous nematocides, there is a strong drive to finding natural product-based alternatives with the aim of containing nematode pests in agriculture. the metabolites produced by the fungal endophyte fusarium oxysporum 162 when cultivated on rice media were isolated and their structures elucidated. eleven compounds were obtained, of which six were isolated from a fusarium spp. for the first time. the three most potent nematode-antagonistic compounds, 4-hydroxy ...201727990770
draft genome sequence of rhizobium sp. strain tbd182, an antagonist of the plant-pathogenic fungus fusarium oxysporum, isolated from a novel hydroponics system using organic fertilizer.rhizobium sp. strain tbd182, isolated from a novel hydroponics system, is an antagonistic bacterium that inhibits the mycelial growth of fusarium oxysporum but does not eliminate the pathogen. we report the draft genome sequence of tbd182, which may contribute to elucidation of the molecular mechanisms of its fungistatic activity.201728302768
defense peptide repertoire of stellaria media predicted by high throughput next generation sequencing.being perfectly adapted to diverse environments, chickweed (stellaria media (l.) vill), a ubiquitous garden weed, grows widely in europe and north america. as opposed to the model plants, many weeds, and s. media in particular, have been poorly studied, although they are likely to contain promising components of immunity and novel resistance genes. in this study, for the first time rna-seq analysis of healthy and infected with fusarium oxysporum chickweed seedlings, as well as de novo transcript ...201728038935
the tomato i gene for fusarium wilt resistance encodes an atypical leucine-rich repeat receptor-like protein whose function is nevertheless dependent on sobir1 and serk3/bak1.we have identified the tomato i gene for resistance to the fusarium wilt fungus fusarium oxysporum f. sp. lycopersici (fol) and show that it encodes a membrane-anchored leucine-rich repeat receptor-like protein (lrr-rlp). unlike most other lrr-rlp genes involved in plant defence, the i gene is not a member of a gene cluster and contains introns in its coding sequence. the i gene encodes a loopout domain larger than those in most other lrr-rlps, with a distinct composition rich in serine and thre ...201727995670
stilbene derivatives from photorhabdus temperata sn259 and their antifungal activities against phytopathogenic fungi.chemical investigation of an insect pathogenic enterobacterium, photorhabdus temperata sn259, led to the isolation and identification of seven metabolites, which include three new compounds, 3-hydroxy-2-isopropyl-5-phenethylphenyl carbamate, 1, 2-(1-hydroxypropan-2-yl)-5-[2-phenylethyl]benzene-1,3-diol, 2, 2-(1-hydroxypropan-2-yl)-5-[(e)-2-phenylethenyl]benzene-1,3-diol, 3, and four known metabolites (4-7). their structures were elucidated on the basis of ms and nmr data and by comparison with t ...201727960253
recombinant expression, characterization, and application of a phospholipase b from fusarium oxysporum.in this study, a gene encoding a putative lipase from fusarium oxysporum was optimized via codon optimization and expressed in pichia pastoris km71. the gene product was identified as a phospholipase b (plb). the engineered p. pastoris was further cultured in a 3.6-l bioreactor. after optimization of the induction conditions, this system produced 6.6mgml(-1) protein and 6503.8uml(-1) plb activity in the culture medium. efficient expression of this plb in p. pastoris should reduce the costs of pr ...201727940286
a gbs-snp-based linkage map and quantitative trait loci (qtl) associated with resistance to fusarium oxysporum f. sp. niveum race 2 identified in citrullus lanatus var. citroides.a major qtl for resistance to fusarium oxysporum f. sp. niveum race 2 was mapped to a narrow 1.2 mb interval using a high-density gbs-snp linkage map, the first map of citrullus lanatus var. citroides. fusarium wilt, a fungal disease caused by fusarium oxysporum f. sp. niveum (fon), devastates watermelon crop production worldwide. several races, which are differentiated by host range, of the pathogen exist. resistance to fon race 2, a particularly virulent strain prevalent in the united states, ...201727803951
preparation, characterization, and bio-efficacy evaluation of controlled release carbendazim-loaded polymeric nanoparticles.synthesis and controlled release study of polymeric nanoformulation of carbendazim (2-benzimidazole carbamic acid methyl ester) using chitosan and pectin is reported in this article. the formulation was subjected to morphological, physiological, in vitro fungicide release and bio-efficacy evaluation studies. the average size of nanoparticles was found to be in the range of 70-90 nm as confirmed by transmission electron microscopy. the in vitro fungicide release of nanoformulated carbendazim was ...201727761863
first report of fusarium oxysporum species complex infection in zebrafish culturing system.fusarium oxysporum species complex (fosc) is a highly diverse fungus. recently, f. oxysporum infection was identified from zebrafish (danio rerio) culturing system in korea. initially, a rapid whitish smudge was appeared in the water with the fungal blooming on walls of fish tanks. microscopic studies were conducted on fungal hyphae, colony pigmentation and chlamydospore formation and the presence of macro- and microspores confirmed that the isolated fungus as f. oxysporum. furthermore, isolated ...201727451953
races of the celery pathogen fusarium oxysporum f. sp. apii are polyphyletic.fusarium oxysporum species complex (fosc) isolates were obtained from celery with symptoms of fusarium yellows between 1993 and 2013 primarily in california. virulence tests and a two-gene dataset from 174 isolates indicated that virulent isolates collected before 2013 were a highly clonal population of f. oxysporum f. sp. apii race 2. in 2013, new highly virulent clonal isolates, designated race 4, were discovered in production fields in camarillo, california. long-read illumina data were used ...201727938244
multiple evolutionary trajectories have led to the emergence of races in fusarium oxysporum f. sp. lycopersici.race 1 isolates of fusarium oxysporum f. sp. lycopersici (fol) are characterized by the presence of avr1 in their genomes. the product of this gene, avr1, triggers resistance in tomato cultivars carrying resistance gene i in fol race 2 and race 3 isolates, avr1 is absent, and hence they are virulent on tomato cultivars carrying i in this study, we analyzed an approximately 100-kb genomic fragment containing the avr1 locus of fol race 1 isolate 004 (fol004) and compared it to the sequenced genome ...201727913420
synthesis and fungicidal activity of tryptophan analogues - the unexpected calycanthaceous alkaloid derivatives.a series of 21 n-protected tryptophan derivatives were synthesised from tryptophan in good yields. their structures were characterised by ir, (1)h nmr, (13)c nmr, dept (90° and 135°) and ms analysis. the synthesised compounds were evaluated against a wide variety of plant pathogen fungi. compounds a19 and a21 displayed activity against fusarium oxysporum (f. oxysporum), and compound a21 showed high activity against f. oxysporum and eggplant verticillium, with ec50 values of 58.27 and 77.39 μg ml ...201727653454
antifungal activities of selected essential oils against fusarium oxysporum f. sp. lycopersici 1322, with emphasis on syzygium aromaticum essential oil.the antifungal effects of four essential oils viz., clove (syzygium aromaticum), lemongrass (cymbopogon citratus), mint (mentha × piperita) and eucalyptus (eucalyptus globulus) were evaluated against wilt causing fungus, fusarium oxysporum f. sp. lycopersici 1322. the inhibitory effect of oils showed dose-dependent activity on the tested fungus. most active being the clove oil, exhibiting complete inhibition of mycelial growth and spore germination at 125 ppm with ic50 value of 18.2 and 0.3 ppm, ...201727876218
identification, transcriptional and functional analysis of heat-shock protein 90s in banana (musa acuminata l.) highlight their novel role in melatonin-mediated plant response to fusarium wilt.as one popular fresh fruit, banana (musa acuminata) is cultivated in the world's subtropical and tropical areas. in recent years, pathogen fusarium oxysporum f. sp. cubense (foc) has been widely and rapidly spread to banana cultivated areas, causing substantial yield loss. however, the molecular mechanism of banana response to foc remains unclear, and functional identification of disease-related genes is also very limited. in this study, nine 90 kda heat-shock proteins (hsp90s) were genomewide i ...201727627033
agroindustrial composts to reduce the use of peat and fungicides in the cultivation of muskmelon seedlings.environmental concerns about peat extraction in wetland ecosystems have increased. therefore, there is an international effort to evaluate alternative organic substrates for the partial substitution of peat. the aim of this work was to use different composts (c1-c10) obtained from the fruit and vegetable processing industry (pepper, carrot, broccoli, orange, artichoke residues, sewage sludge (citric and pepper) and vineyard pruning wastes) to produce added-value composts as growing media with su ...201727197924
success evaluation of the biological control of fusarium wilts of cucumber, banana, and tomato since 2000 and future research strategies.the fusarium wilt caused by fusarium oxysporum strains is the most devastating disease of cucumber, banana, and tomato. the biological control of this disease has become an attractive alternative to the chemical fungicides and other conventional control methods. in this review, the research trends and biological control efficiencies (bce) of different microbial strains since 2000 are reviewed in detail, considering types of microbial genera, inoculum application methods, plant growth medium and ...201726810104
genetic analysis and chromosome mapping of resistance to fusarium oxysporum f. sp. niveum (fon) race 1 and race 2 in watermelon (citrullus lanatus l.).fusarium wilt (fw) caused by fusarium oxysporum f. sp. niveum (fon) is the major soilborne disease of watermelon (citrullus lanatus l.). the development and deployment of resistant cultivars is generally considered to be an effective approach to control fw. in this study, an f8 population consisting of 103 recombinant inbred lines derived from a cross between the cultivar 97103 and a wild accession pi 296341-fr was used for fon race 1 and race 2 fungal inoculations. one major qtl on chromosome 1 ...201726347205
koninginins r-s from the endophytic fungus trichoderma koningiopsis.two new metabolites named koninginins r-s (1-2) were isolated from the culture of trichoderma koningiopsis yim ph30002. their chemical structures were elucidated by the extensive spectroscopic analysis. these isolated compounds showed certain antifungal activities against phytopathogens, fusarium flocciferum and fusarium oxysporum.201727784167
sequencing of individual chromosomes of plant pathogenic fusarium oxysporum.a small chromosome in reference isolate 4287 of f. oxysporum f. sp. lycopersici (fol) has been designated as a 'pathogenicity chromosome' because it carries several pathogenicity related genes such as the secreted in xylem (six) genes. sequence assembly of small chromosomes in other isolates, based on a reference genome template, is difficult because of karyotype variation among isolates and a high number of sequences associated with transposable elements. these factors often result in misassemb ...201727919652
genetic mapping of resistance to fusarium oxysporum f. sp. tulipae in tulip.fusarium oxysporum is a major problem in the production of tulip bulbs. breeding for resistant cultivars through a conventional approach is a slow process due to the long life cycle of tulip. until now, marker-assisted selection (mas) has been hampered by the large genome size and the absence of a genetic map. this study is aimed at construction of the first genetic map for tulip and at the identification of loci associated with resistance to f. oxysporum. a cross-pollinated population of 125 in ...201725983613
endophytic fungi associated with monarda citriodora, an aromatic and medicinal plant and their biocontrol potential.the food and agriculture organization has estimated that every year considerable losses of the food crops occur due to plant diseases. although fungicides are extensively used for management of plant diseases, they are expensive and hazardous to the environment and human health. alternatively, biological control is the safe way to overcome the effects of plant diseases and to sustain agriculture. since monarda citriodora cerv. ex lag. (lamiaceae/labiatae) is known for its antifungal properties, ...201728398103
application of surface modified nano ferrite nickel in catalytic reaction (epoxidation of alkenes) and investigation on its antibacterial and antifungal activities.newly, magnetic nanoparticles have extensively been used as alternative catalyst supports, in the view of their high surface area which results in high catalyst loading capacity, high dispersion, low toxicity, environmental preservation, distinguished stability, and suitable catalyst reusing. in the present study, the magnetite nanoparticles, nife2o4@ag and nife2o4@mo, were synthesized and characterized. the antimicrobial activities and catalytic properties of synthesized nanoparticles were test ...201728575933
lipopeptides produced by bacillus subtilis as new biocontrol products against fusariosis in ornamental plants.in this study, we have investigated the effects of three lipopeptides (fengycin, surfactin and mycosubtilin) produced by different strains of bacillus subtilis against the phytopathogenic fungi fusarium oxysporum f. sp. iridacearum, which affects the ornamental bulb plant populations of iris sp. the antifungal effects were tested using minimum inhibitory concentration assay, determination of mycelium growth and spore germination inhibition rates. also, in vivo tests on infected rhizomes and scan ...201728528498
isolated bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing pcr.plant growth-promoting bacteria (pgpb) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. in this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. based on 16s rdna and biolog test analysis, the 330-2 strain was identified as bacillus subtilis. the strain produced indole-3-acetic acid, siderophores, lytic e ...201728496135
plipastatin and surfactin coproduction by bacillus subtilis pb2-l and their effects on microorganisms.to convert the lipopeptide non-producer strain bacillus subtilis pb2 into a plipastatin and surfactin coproducer, a gene expression cassette composed of a constitutive promoter (p43), functional gene sfp, and pleiotropic regulatory gene degq was integrated into the chromosomal amye locus of strain b. subtilis pb2 by homologous recombination, which generated a plipastatin and surfactin co-producer. thirteen plipastatins and fifteen surfactins were identified in lipopeptide extracts using analytic ...201728477175
rnai-mediated silencing of map kinase signalling genes (fmk1, hog1, and pbs2) in fusarium oxysporum reduces pathogenesis on tomato plants.fusarium oxysporum is a soil-borne plant fungal pathogen, and causes colossal losses in several crop plants including tomato. effective control measures include the use of harmful fungicides and resistant cultivars, but these methods have shown limited success. conventional methods to validate fungal pathogenic genes are labour intensive. therefore, an alternative strategy is required to efficiently characterize unknown pathogenic genes. rna interference (rnai) has emerged as a potential tool to ...201728800849
essential oils as biocides for the control of fungal infections and devastating pest (tuta absoluta) of tomato (lycopersicon esculentum mill.).thymus capitatus and tetraclinis articulata essential oils as well their major components (carvacrol and α-pinene) were evaluated for their antifungal and insecticidal activities. both oils showed good in vitro antifungal activity against fusarium oxysporum, aspergillus niger, penicillium sp., alternaria alternata, and botrytis cinerea, the fungi causing tomato rot. in vivo results indicate the efficacies of both essential oils and carvacrol of reduce postharvest fungal pathogens, such as b. cin ...201728422413
identification and antifungal activity of compounds from the mangrove endophytic fungus aspergillus clavatus r7.two new coumarin derivatives, 4,4'-dimethoxy-5,5'-dimethyl-7,7'-oxydicoumarin (1), 7-(γ,γ-dimethylallyloxy)-5-methoxy-4-methylcoumarin (2), a new chromone derivative, (s)-5-hydroxy-2,6-dimethyl-4h-furo[3,4-g]benzopyran-4,8(6h)-dione (5), and a new sterone derivative, 24-hydroxylergosta-4,6,8(14),22-tetraen-3-one (6), along with two known bicoumarins, kotanin (3) and orlandin (4), were isolated from an endophytic fungus aspergillusclavatus (collection no. r7), isolated from the root of myoporum b ...201728825634
a novel photosensitization treatment for the inactivation of fungal spores and cells mediated by curcumin.the global concerns regarding the emergence of fungicide-resistant strains and the impact of the excessive use of fungicidal practises on our health, food, and environment have increased, leading to a demand for alternative clean green technologies as treatments. photosensitization is a treatment that utilises a photosensitiser, light and oxygen to cause cell damage to microorganisms. the effect of photosensitization mediated by curcumin on aspergillus niger, aspergillus flavus, penicillium gris ...201728623822
coupling the pretreatment and hydrolysis of lignocellulosic biomass by the expression of beta-xylosidases.thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. the improvement of lignocellulosic enzyme cocktails has been significant in the recent years. although the needs for the reduction of the energy intensity and chemical consumption in the pretreatment step are well known, the reduction of the severity of the process strongly affects the enzymatic hydrolysis yield. to explore t ...201728710860
chemical composition and antifungal activity of hedera helix leaf ethanolic extract.the 50% ethanol extract obtained from hedera helix leaves was investigated regarding the presence and quantity of polyphenols, sterols and in vitro antifungal activity against phytopathogenic fungi. the chemical analysis revealed the presence of rutin, quercetin and kaempferol in the non-hydrolysed sample and quercetin and kaempferol in the hydrolysed sample and stigmasterol in the ivy leaf extract (nonhydrolysed sample). the antifungal activity against phytopathogenic fungi (aspergillus niger, ...201728605982
isolation of a potential biocontrol agent paenibacillus polymyxa nsy50 from vinegar waste compost and its induction of host defense responses against fusarium wilt of cucumber.fusarium wilt caused by fusarium oxysporum f. sp. cucumerinum (foc) is one of the major destructive soil-borne diseases infecting cucumber. in this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. the 16s rdna gene demonstrated that the biocontrol agents were paenibacillus polymyxa (p. polymyxa), bacillus amyloliquefaciens (b. amyloliquefaciens) and bacillus ...201728647117
nutritional composition and phytochemical, antioxidative, and antifungal activities of pergularia tomentosa l.crude extracts from a medicinal tunisian plant, pergularia tomentosa l., were the investigated natural material. butanolic extract of roots analyzed with ir spectra revealed the presence of hydroxyl, alcoholic, and carboxylic groups and sugars units. analysis of some secondary metabolites, total phenolic, flavonoids, flavonols, and procyanidins, was performed using different solvents following the increased gradient of polarity. fruits and leaves contained the highest amounts of all these compou ...201728409159
immobilization of a cutinase from fusarium oxysporum and application in pineapple flavor synthesis.in the present study, the immobilization of a cutinase from fusarium oxysporum was carried out as cross-linked enzyme aggregates. under optimal immobilization conditions, acetonitrile was selected as precipitant, utilizing 9.4 mg protein/ml and 10 mm glutaraldehyde as cross-linker. the immobilized cutinase (imfocut5a) was tested in isooctane for the synthesis of short-chain butyrate esters, displaying enhanced thermostability compared to the free enzyme. pineapple flavor (butyl butyrate) synthes ...201728403608
methanolic extract of plumbago zeylanica - a remarkable antibacterial agent against many human and agricultural pathogens.the current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of plumbago zeylanica.201728392958
in vitro antifungal, probiotic, and antioxidant functional properties of a novel lactobacillus paraplantarum isolated from fermented dates in saudi arabia.fermented foods produced using dates are used in gulf countries as beneficial and healthful foods. the beneficial microbial flora in fermented dates contributes to maintaining the nutritional properties of dates by preventing the growth of spoilage fungi. here, we examined the antifungal, probiotic, and antioxidant properties of the novel lactobacillus strain d-3 isolated from fermented dates.201728480570
chemical composition and antifungal activity of the essential oil of zhumeria majdae, heracleum persicum and eucalyptus sp. against some important phytopathogenic fungi.application of essential oils of medicinal plants is considered a safe and acceptable method for plant disease management to protect plants from pathogenic microorganisms. thus, in recent study, essential oils (eos) from zhumeria majdae, heracleum persicum (two iranian endemic plants) and eucalyptus sp. were assayed for their antifungal potential against ten phytopathogenic fungi, including fusarium graminearum, fusarium asiaticum, fusarium redolens f.sp. dianthus, fusarium verticillioides, fusa ...201728757068
isolation, identification, optimization, and metabolite profiling of streptomyces sparsus vsm-30.deep sea sediment samples of bay of bengal (visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. the actinomycetes strain vsm-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. it was identified as streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. response surface methodology regression analysis was carried out to fi ...201728669076
nonsteroidal anti-inflammatory drugs (nsaids) inhibit the growth and reproduction of chaetomium globosum and other fungi associated with water-damaged buildings.indoor mold due to water damage causes serious human respiratory disorders, and the remediation to homes, schools, and businesses is a major expense. prevention of mold infestation of building materials would reduce health problems and building remediation costs. nonsteroidal anti-inflammatory drugs (nsaids) inhibit yeasts and a limited number of filamentous fungi. the purpose of this research was to determine the possible inhibitory activity of nonsteroidal anti-inflammatory drugs (nsaids) on g ...201728795317
in vitro antimicrobial activities and mechanism of 1-octen-3-ol against food-related bacteria and pathogenic fungi.1-octen-3-ol, known as mushroom alcohol, is a natural product extracted from fungi and plants. its antimicrobial activities against five common food-related bacteria and two pathogenic fungi were evaluated in this paper, including staphylococcus aureus, bacillus subtilis, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, fusarium tricinctum and fusarium oxysporum. the results showed that 1-octen-3-ol had a strong antibacterial activity against the tested bacteria, especially ...201728794307
c-coordinated o-carboxymethyl chitosan metal complexes: synthesis, characterization and antifungal efficacy.a novel type of o-carboxymethyl chitosan schiff bases (o-cspx) was synthesized via a condensation reaction. after the coordination reaction of cupric ions, zinc ions and nickel ions, metal complexes (o-cspx-m) were achieved. the theoretical structure of o-cspx-m calculated by gaussian 09 reveals that the copper ions and nickel ions underwent dsp(2) hybridization, the zinc ions underwent sp(3) hybridization, and they all coordinated by the carbon atom in the p-π conjugate group. then, the structu ...201728774807
production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source.the aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi (wrf) using peels or discarded potato as the sole nutrient source.201728718996
infection courts in watermelon plants leading to seed infestation by fusarium oxysporum f. sp. niveum.fusarium wilt incited by fusarium oxysporum f. sp. niveum (fon) is a seed-transmitted disease that causes significant yield loss in watermelon production. the pathogen may infect watermelon seeds latently, which can be an important inoculum source and contribute to severe disease outbreak. however, information regarding infection courts of fon leading to infestation of watermelon seeds is limited. to determine how seeds in watermelon fruit can be infested by fon during the watermelon growing sea ...201728414630
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