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a novel enzyme immunoassay for conidia of fusarium oxysporum f.sp. cucumerinum f504.this study was undertaken to develop a new tool to study fusarial diseases of plants. micro- and macro-conidia of a strain (f504) of fusarium oxysporum were isolated and antiserum against the conidia was elicited in rabbits. a highly specific and sensitive competitive-type enzyme-linked immunosorbent assay (elisa) for conidia of the strain was developed using the antiserum with beta-d-galactosidase-labeled anti-rabbit igg as the secondary antibody and conidia fragments of the strain as antigen a ...19892511393
the mitochondrial genome of fusarium oxysporum.physical and genetic maps have been constructed for mtdna from strains of the fungus fusarium oxysporum representing three pathogenically specialized forms. all three mtdna maps are circular. their sizes are 45 kb for f. oxysporum f.sp. raphani and 52 kb for both f. oxysporum f.sp. conglutinans and f. oxysporum f.sp. matthioli. the genetic loci for cytochrome b, the mitochondrial 25s ribosomal rna and cytochrome oxidase subunit ii, have been identified and are similarly arranged on the three gen ...19892550987
disseminated fusarium oxysporum infection in patient with heatstroke. 19892565468
immunosuppressive effects of dietary wortmannin on rats and mice.in order to assess the effects of the fungal toxin wortmannin on the immune system, rats and mice were fed wortmannin-containing cultures of fusarium oxysporum for 1 or 2 weeks. wortmannin caused significant decreases in thymic weight, thymic lymphocyte numbers, serum igg and igm levels, the primary humoral response to t-dependent and t-independent antigens and the proliferative response of spleen cells to pokeweed mitogen. in vitro administration of wortmannin did not produce evidence of cytoto ...19892628478
ultrastructural study of galacturonic acid distribution in some pathogenic fungi using gold-complexed aplysia depilans gonad lectin.aplysia gonad lectin, isolated from the mollusc aplysia depilans, was successfully conjugated to colloidal gold and used for ultrastructural detection of galacturonic acids in some pathogenic fungi. these sugar residues were found to occur in the fibrillar sheath surrounding hyphal cells of ascocalyx abietina and in intravacuolar dense inclusions of this fungus spores. in hyphae and spores of ophiostoma ulmi, galacturonic acids were detected mainly in the outermost wall layers. in contrast, thes ...19892659155
cloning of the nitrate reductase gene (niad) of aspergillus nidulans and its use for transformation of fusarium oxysporum.an heterologous transformation system for the phytopathogenic fungus fusarium oxysporum has been developed based on the use of the aspergillus nidulans nitrate reductase gene (niad). f. oxysporum nia- mutants were easily selected by chlorate resistance. the a. nidulans niad gene was isolated from a gene library by complementation of an a. nidulans niad mutant. the cloned gene is capable of transforming f. oxysporum nia- mutants at a frequency of up to ten transformants per microgram of dna. sout ...19892670677
transformation of seven species of filamentous fungi using the nitrate reductase gene of aspergillus nidulans.a gene transfer system originally developed for fusarium oxysporum has been applied to seven species of filamentous fungi of agricultural and industrial importance. this transformation system relies on the selection of mutants deficient in nitrate reductase by positive screening. such mutants were recovered easily in all the fungi tested--without mutagenic treatments--through their resistance to chlorate. they were transformed by a plasmid vector (pan301) carrying the aspergillus nidulans wild-t ...19892673557
mycotoxin production by fusarium oxysporum and fusarium sporotrichioides isolated from baccharis spp. from brazil.fusarium oxysporum isolated from roots of and soil around baccharis species from brazil produced the trichothecenes t-2 toxin, ht-2 toxin, diacetoxyscirpenol, and 3'-oh t-2 (tc-1), whereas fusarium sporotrichioides from the same source produced t-2 toxin, ht-2 toxin, acetyl t-2, neosolaniol, tc-1, 3'-oh ht-2 (tc-3), iso-t-2, t-2 triol, t-2 tetraol, and the nontrichothecenes moniliformin and fusarin c. several unknown toxins were found but not identified. not found were macrocyclic trichothecenes ...19892705770
acute pathological effects on rats of orally administered wortmannin-containing preparations and purified wortmannin from fusarium oxysporum.the pathological effects in rats of orally administered wortmannin, a mycotoxin produced by fusarium oxysporum, were studied. weanling rats were fed a wortmannin-containing fungal culture for 4 or 5 days, or were given by intragastric gavage a single, lethal dose of extracts of a wortmannin-containing fungal culture or pure toxin. haemoglobinuria, necrosis of lymphoid tissues and death occurred in rats fed the fungal culture. administration by gavage of extracts of the wortmannin-containing cult ...19892786490
extraction and purification of dna from phytopathogenic soil-inhabiting fusarium species.working quantities of good quality dna was obtained from three pathogenic forms of the soil-inhabiting fungus, fusarium oxysporum (fo), which causes economically significant diseases in tomato crops. the mitochondrial, nuclear and ribosomal fractions from each of the dnas were separated by cesium chloride gradient centrifugations. the data suggest a decrease in nuclear dna and an increase in mitochondrial dna correlated with increasing pathogenicity among the three pathogens examined. ribosomal ...19892809080
antifungal susceptibility of 44 clinical isolates of fusarium species determined by using a broth microdilution method.the mics and minimum fungicidal concentrations of amphotericin b, natamycin, miconazole, itraconazole, and flucytosine against 17 isolates of fusarium solani, 14 isolates of fusarium moniliforme, 10 isolates of fusarium oxysporum, and 3 isolates of fusarium semitectum were determined by a broth microdilution method. amphotericin b and natamycin were the most active agents tested and failed to show any inoculum size effect. in contrast, miconazole and itraconazole showed poor inhibitory and fungi ...19892817867
fusarium oxysporum keratitis.we describe herein a patient with a rare occurrence of mycotic keratitis caused by fusarium oxysporum. the fungus destroyed the cornea, and a perforating corneal graft had to be performed. the drug of choice in such diagnosed cases is natamycin. a comparison between fusarium oxysporum and fusarium solani is discussed.19892919034
soluble, nitrate/nitrite-inducible cytochrome p-450 of the fungus, fusarium oxysporum.both soluble and microsomal fractions of fusarium oxysporum contain cytochrome p-450(p-450). we report here that the p-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal p-450 was synthesized regardless of the medium compositions. the reduced-co complex of the soluble p-450 exhibited an absorption spectrum that is different from that of the microsomal counterpart. these results indicate that the soluble p-450 is distinct fr ...19892924900
fosfonochlorin, a new antibiotic with spheroplast forming activity.a new antibiotic, fosfonochlorin, was found in the culture filtrate of four strains of fungi freshly isolated from soil samples. these strains were identified as fusarium avenaceum, fusarium oxysporum, fusarium tricinctum and talaromyces flavus. fosfonochlorin was a low molecular weight antibiotic (mw 158), soluble in water and methanol, but insoluble in acetone, ethyl acetate and chloroform. it was named after its possession of phosphorus and chlorine atoms, each one molar in its structure. the ...19892925511
osmotic, biomass, and oxygen effects on the growth rate of fusarium oxysporum using a dissolved-oxygen-controlled turbidostat.the system described is a modified hospodka's turbidostat. this device helps to measure the maximum growth rate of fungi in steady-state aerobic conditions with defined and independent concentration of dissolved oxygen, biomass, and substrate even unlimited. the principle consists of a turbidostat controlled by the dissolved oxygen concentration. the inlet medium pump operates when the dissolved oxygen concentration falls below the set point value. this method allows us to study independently ef ...198918587971
the pleiotropic phenotype of tomato cells selected for altered response to fusarium oxysporium f. sp. lycopersici cell wall components.with the aim of better understanding in vitro host-parasite interactions, tomato cell lines selected for altered response to fusarium oxysporum f. sp. lycopersici cell wall components were further characterized. particularly, their behaviour in dual culture in regard to both fungal inhibition and peroxidase activation was analysed and selected, and control cell clones were screened for esopolysaccharide content and toxin tolerance. interclonal differences in growth response to 2,4-d and dmso and ...198924225830
an isozyme marker for resistance to race 3 of fusarium oxysporum f. sp. lycopersici in tomato.the inheritance and the linkage relationship of resistance to race 3 of fusarium oxysporum f. sp. lycopersici derived from lycopersicon pennellii (la 716) were analyzed in an interspecific backcross to l. esculentum. progeny from each backcross (bc1) individual were in-oculated with race 3 and their response was measured according to a visual rating system; progeny responses were used to calculate a mean disease rating for each bc1 individual. the frequency distribution of the disease ratings wa ...198924225675
an rflp marker in tomato linked to the fusarium oxysporum resistance gene i2.the locus, i2, which in tomato confers resistance against fusarium oxysporum f. sp. lycopersici race 2, was introgressed into lycopersicon esculentum from the wild species l. pimpinellifolium (p.i. 126915). we searched for restriction fragment length polymorphisms (rflps) between nearly isogenic lines (nils) in clones that map to the region introgressed from the wild species. since i2 maps to chromosome 11, we used dna clones from this chromosome as hybridization probes to southern blots contain ...198924225839
somaclonal variant uc-t3: the expression of fusarium wilt resistance in progeny arrays of celery, apium graveolens l.first generation (s1) progeny, second generation (s2) progeny, and backcross (bc) progeny of a celery (apium graveolens l. var. dulce) somaclonal variant, uc-t3, were evaluated for resistance to the fungus fusarium oxysporum f. sp. apii, race 2 (foa2). chisquare analysis of s1 progeny showed that the expression of resistance did not fit a single-locus model. s2 progeny means were similar among families, except in a heavily infested field. the lowest ranking s2 family in both the lightly infested ...199024220975
genetic analysis of resistances to races 1 and 2 of fusarium oxysporum f. sp. lycopersici from the wild tomato lycopersicon pennellii.resistance to race 3 of fusarium wilt in the wild tomato lycopersicon pennellii (la 716) was previously found to be controlled by one major locus, i-3, tightly linked to got-2 on chromosome 7. this accession was also found to carry resistance to races 1 and 2; a genetic analysis of these resistances is reported in this paper. this analysis proceeded in two steps. first, allelism tests demonstrated that race 1 and 2 resistances carried by l. pennellii were not allelic to the i and i-2 genes origi ...199024226578
subcellular localization of chitinase and of its potential substrate in tomato root tissues infected by fusarium oxysporum f. sp. radicis-lycopersici.antiserum raised against a tomato (lycopersicon esculentum mill.) chitinase (molecular mass of 26 kilodaltons) was used as a probe to study the subcellular localization of this enzyme in tomato root tissues infected with fusarium oxysporum f. sp. radicis-lycopersici. a time-course experiment revealed that chitinase accumulated earlier in the incompatible interaction than in the compatible one. however, in both systems, chitinase deposition was largely correlated with pathogen distribution. the e ...199016667378
implication of pectic components in cell surface interactions between tomato root cells and fusarium oxysporum f. sp. radicis-lycopersici: a cytochemical study by means of a lectin with polygalacturonic acid-binding specificity.aplysia gonad lectin, a polygalacturonic acid-binding lectin isolated from the sea mollusc aplysia depilans, was complexed to colloidal gold and used for localizing polygalacturonic-acid-containing molecules in tomato root tissues infected with fusarium oxysporum f. sp. radicis-lycopersici (forl). colonization of host tissues by forl was associated with striking wall modifications including disruption and even loss of middle lamellae. according to the labeling pattern observed in host wall areas ...199016667417
regulation of sugar transport systems in fusarium oxysporum var. lini.fusarium oxysporum var. lini (atcc 10960) formed a facilitated diffusion system for glucose (k(s), about 10 mm) when grown under repressed conditions. under conditions of derepression, the same system was present together with a high-affinity (k(s), about 40 mum) active system. the maximum velocity of the latter was about 5% of that of the facilitated diffusion system. the high-affinity system was under the control of glucose repression and glucose inactivation. when lactose was the only carbon ...199016348256
keratinophilic fungi and other moulds associated with air-dust particles from egypt.one-hundred and eleven species and three species varieties belonging to 39 genera were collected from 50 dust samples on the five media used at 28 degrees c. using the hair-baiting technique with horse hair, 10 species of chrysosporium were isolated: c. asperatum, c. state of arthroderma tuberculatum, c. indicum, c. inops, c. keratinophilum, c. merdarium, c. pannorum, c. queenslandicum, c. tropicum and c. xerophilum. true dermatophytes were isolated: trichophyton verrucosum and trichophyton sp. ...19901702081
antifungal activity of some phenolic compounds on fusarium oxysporum f. sp. psidii causing guava wilt. 19902095362
formation of deglycosylated alpha-l-fucosidase by endo-beta-n-acetylglucosaminidase in fusarium oxysporum.two forms of alpha-l-fucosidase, deglycosylated and glycosylated, were found in the fucose-inducing culture broth of fusarium oxysporum. endo-beta-n-acetylglucosaminidase was also found in the same culture broth. the deglycosylated alpha-l-fucosidase was purified from the culture broth to homogeneity on polyacrylamide disc gel electrophoresis and analytical ultracentrifugation. purified deglycosylated alpha-l-fucosidase was compared in chemical composition and immunological homology with glycosy ...19902111117
efficacy of some antibiotics on fusarium oxysporum f. sp. psidii causing wilt disease of guava. 19902131331
hypotensive effects on spontaneously hypertensive rats and antifungal activity on various species of fusarium oxysporum of diethylstilbestrol-related compounds.the diethylstilbestrol-related compounds 3,3'-dihydroxy-alpha, beta-diethyldiphenylethane (i), diethylstilbestrol (ii) and hexestrol (iii) showed hypotensive effects on spontaneously hypertensive rats (shr) and antifungal activities against all fusarium oxysporum sp. tested. as previously reported, i had strong hypotensive action on normotensive rats at the dose of 10 mg/kg, while ii and iii showed weak hypotensive effects on these rats at the same dose. in this work, all three compounds also ha ...19902176575
biological activities of racemomycin-b, beta-lysine rich streptothricin antibiotic, the main component of streptomyces lavendulae op-2.racemomycin-b (rm-b), the main component of streptomyces lavendulae op-2 which is the basis of 50% of the antibiotics produced, is a streptothricin antibiotic which contains three beta-lysine moieties in the molecule. rm-b had antimicrobial activity against plant-pathogenic microorganisms and growth-inhibitory activity against the root of brassica rapa l. at the concentration of 50 ppm. it strongly inhibited the growth of pseudomonas syringae pv. tabaci ifo-3508 (minimum inhibitory concentration ...19902177682
survival and inoculum potential of conidia and chlamydospores of fusarium oxysporum f.sp. lini in soil.the kinetics of survival and inoculum potential of fusarium oxysporum f.sp. lini were studied in soil. two types of inoculum were compared: microconidia freshly harvested from a laboratory-grown culture and microchlamydospores produced in sterilized soil. introduced at the same inoculum densities into a natural soil, the two types of inoculum showed similar behavior; the inoculum densities changed little with time, at least during 100 days. however, the two types of inoculum did differ in diseas ...19902245379
isoverrucarol production by fusarium oxysporum cjs-12 isolated from corn.isoverrucarol (3,15-dihydroxy-12,13-epoxy-trichothec-9-ene) was isolated and purified from wheat cultures of a toxic strain of fusarium oxysporum cjs-12. the toxin was characterized by thin-layer chromatography, gas chromatography-mass spectrometry, and 1h and 13c nuclear magnetic resonance spectrometry. isoverrucarol caused toxic effects in rats, including loss of appetite, bodily weakness, severe mucosae of the stomach, and death, when administered orally at 10 and 20 mg/kg of body weight. the ...19902310181
in vivo rearrangement of foreign dna by fusarium oxysporum produces linear self-replicating plasmids.particular combinations of fungal strains and transformation vectors allow for fungal rearrangement of normally integrative plasmids, resulting in the creation of linear self-replicating plasmids in fusarium oxysporum. the rearrangement results in the addition of fungal dna, including telomere consensus sequences, to plasmid termini. the mechanism by which this rearrangement occurs is unclear, but it has similarities to extrachromosomal gene amplification. a dna fragment which allows for linear ...19902345140
acute toxic effects of an isolate of moniliformin-producing fusarium oxysporum and purified moniliformin on rats.an isolate of fusarium oxysporum (mt-6) was obtained from pasture soil in new zealand in 1987. the isolate was grown on rice and fed to rats in a ratio of 50% and 10% of a complete diet. all rats died within 16 hr and showed mild intestinal and thymic hemorrhage. the major identifiable toxin was moniliformin found at a concentration of 9 mg/g in the rice culture. purified crystals of moniliformin fed to rats at concentrations of 8, 5 and 3 mg/g of rat diet caused hemorrhaging in the intestines a ...19902353842
fusarium endocarditis involving aortic valve following coronary artery surgery.this article describes a case of infective aortic valve endocarditis due to fusarium oxysporum occurring 4 years after coronary artery bypass grafting. it is the first reported case of endocarditis caused by fusarium.19902354707
sti35, a stress-responsive gene in fusarium spp.a stress-induced mrna was identified in the phytopathogenic fungus fusarium oxysporum f. sp. cucumerinum. treatment of the fungus with ethanol resulted in the induction of a major mrna species encoding a protein of approximate mr 37,000. a full-length cdna clone of the induced message was obtained. rna blot analysis indicated that the mrna was induced by various other stresses, including treatment with copper(ii) chloride and heat (37 degrees c). however, it was not greatly induced by treatment ...19902376567
production, thermal stability and immobilisation of inulinase from fusarium oxysporum.fusarium oxysporum produced maximum extracellular inulinase after 9 days of its growth at 25 degrees c on a medium (ph 5.5) containing 3% fructan and 0.2% sodium nitrate. the level of this enzyme decreased on the addition of either glucose, fructose, galactose or sucrose to f. oxysporum already growing on a fructan-containing medium. a significant increase in invertase production which resulted in an increase of the invertase/inulinase (s/i) ratio, was observed on addition of inulin to this fung ...19901366387
pathological relationship of ditylenchus dipsaci and fusarium oxysporum f. sp. medicaginis on alfalfa.ditylenchus dipsaci and fusarium oxysporum f. sp. medicaginis synergistically affected the mortality and plant growth of ranger alfalfa, a cultivar susceptible to stem nematode and fusarium wilt. the nematode-fungus relationship had an additive effect on mortality and plant growth of lahontan (nematode resistant and fusarium wilt susceptible) and of moapa 69 (nematode susceptible and fusarium wilt resistant). mortality rates were 13, 16, 46, and 49% for ranger; 4, 18, 26, and 28% for lahontan; a ...199019287729
plant-parasitic nematodes and fungi associated with root rot of peas on prince edward island.eight commercial pea fields on prince edward island were sampled in june and july over a 2-year period (1986-87) to determine soil population densities and the incidence of nematodes and fungi associated with root rot of peas. root lesion nematodes (pratylenchus spp.) were the dominant endoparasitic nematodes recovered from roots and soil. low populations of the northern root-knot nematode (meloidogyne hapla) were also present. tylenchorhynchus spp. and paratylenchus spp. were recovered frequent ...199019287779
interaction between meloidogyne incognita and agrobacterium tumefaciens or fusarium oxysporum f. sp. lycopersici on tomato.agrobacterium tumefaciens stimulated and fusarium oxysporum f. sp. lycopersici inhibited development and reproduction of meloidogyne incognita when applied to the opposite split root of tomato, lycopersicon esculentum cv. tropic, plants. the lowest rate of nematode reproduction occurred after 2,000 juveniles were applied and the fungus was present in the opposite split root. the effects of all three pathogens alone on the growth of roots and shoots of tomato plants were evident, but m. incognita ...199119283119
heterologous expression of the aspergillus nidulans regulatory gene nira in fusarium oxysporum.we have isolated strains of fusarium oxysporum carrying mutations conferring a phenotype characteristic of a loss of function in the regulatory gene of nitrate assimilation (nira in aspergillus nidulans, nit-4 in neurospora crassa). one of these nir- mutants was successfully transformed with a plasmid containing the nira gene of a. nidulans. the nitrate reductase of the transformants is still inducible, although the maximum activity is lower than in the wild type. single and multiple integration ...19911756977
calcium ion regulates the release of lipase of fusarium oxysporum.the lipase production of a plant pathogenic fungus, fusarium oxysporum f. sp. lini suf 402, was induced by fat as the carbon source, and its release was stimulated by the infusion of intracellular free calcium ion with a calcium ionophore, a23187. n-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (w-7, a calmodulin inhibitor) and 1-[n,o-bis(5-isoquinolinesulfonyl)-n-methyl- l-tyrosyl]-4-phenylpiperazine (kn-62, a ca2+/calmodulin dependent protein kinase ii inhibitor) reduced the extracellular r ...19911769973
[cloning a species-specific dna probe from fusarium oxysporum fungus].a method to obtain genus-specific dna probes is suggested. it consists of specific amplification of the intergenic spacer between the 18s and 5.8s ribosomal rna genes, using primers deduced from conservative ribosomal dna sequences. the utility of the method is demonstrated on isolation of the 209 b.p. spacer fragment from the genomic dna of a plant pathogenic fungus fusarium oxysporum.19911813809
influence of temperature on zearalenone production by regional strains of fusarium graminearum and fusarium oxysporum in culture.zearalenone production by fusarium graminearum and fusarium oxysporum was studied under two temperature conditions. incubation at 25 degrees c for 4 weeks enhanced zearalenone synthesis, improving detection of zearalenone-producing strains of fusarium oxysporum. zearalenone production was either totally or partially inhibited when temperature was lowered to 12-14 degrees c during the last 2 weeks of incubation.19911832924
microbial degradation of pendimethalin.microbial degradation of pendimethalin (n-(1-ethylpropyl)-3, 4-dimethyl-2, 6-dinitroaniline) in vitro was studied. fusarium oxysporum and paecilomyces varioti, two soil fungi, in culture media degraded pendimethalin to two metabolites namely n-(1-ethylpropyl)-3, 4-dimethyl-2-nitrobenzene-1, 6-diamine (ii) and 3,4-dimethyl-2, 6-dinitroaniline (iv). rhizoctonia bataticola, another soil fungus, decomposed pendimethalin yielding only the latter metabolite (iv). fungal decomposition of pendimethalin ...19911894917
cytolysis of bacillus subtilis by fusarium oxysporum.growth of fusarium oxysporum on heat-killed bacillus subtilis cells was accompanied by the loss of bacterial cytoplasmic contents, and this 'cytolysis' could be catalysed in heat-treated bacteria by the fungal culture fluids. in electron micrographs the bacterial walls appeared undamaged, and the absence of wall-lytic enzymes was confirmed by use of isolated bacterial walls as substrate. appearance of cytolytic activity in cultures was paralleled by the production of proteolytic activity in the ...19911901903
mycotoxin production on different cultivars and lines of broad bean (vicia faba l.) seeds in egypt.one hundred different cultivars and lines of broad bean (vicia faba l.) seed samples were inoculated with aspergillus flavus link (cmi 102135) to determine varietal differences which may support or resist aflatoxin production. thin-layer chromatographic analysis of the chloroform extracts of the different seed samples revealed that 11 cultivars/lines were highly resistant to seed invasion and aflatoxin production while 9 cultivars/line showed partial resistance. the remaining 80 samples were sus ...19911906134
fusarium oxysporum: status in bioethanol production.fermentation of lignocellulosic materials to ethanol and other solvents provides an alternative way of treating wastes and producing chemical feedstocks and fuel additives. considerable efforts have been made in past 10 years to improve the process based on lignocellulosic biomass and hydrolysate that contains a complex mixture of sugars, decomposition products of sugars, and sometimes the inhibitory levels of soluble lignin. despite the relative abundance of d-xylose in crop and forest residues ...19911913845
effect of ultraviolet-induced mutants of trichoderma harzianum with altered antibiotic production on selected pathogens in vitro.mutants of trichoderma harzianum with altered antibiotic production were isolated using ultraviolet light mutagenesis. these included strains whose activity in a fusarium oxysporum spore germination assay was greater than twice that of the parental strain and one that had no detectable antifungal activity. characterisation of extracellular metabolites of these strains using thin-layer chromatography and gas-liquid chromatography showed that the strains with high activity produced only elevated l ...19911954580
glycerol utilization in fusarium oxysporum var. lini: regulation of transport and metabolism.glycerol was transported in the fungus fusarium oxysporum var. lini by a facilitated diffusion transport system with a half-saturation constant, ks, of 0.5 mm and a maximum velocity, vmax, of 0.9 mmol (g dry wt)-1 h-1 at ph 5 and 25 degrees c. 1,2-propanediol was a competitive inhibitor of glycerol transport, but the cells did not actively accumulate 1,2-propanediol. the transport system was partially constitutive. in cells grown in the presence of glucose, glycerol was not transported, indicati ...19911955848
nucleotide sequence of the unique nitrate/nitrite-inducible cytochrome p-450 cdna from fusarium oxysporum.a cdna clone for the nitrate/nitrite-inducible cytochrome p-450 (p-450) of the fungus fusarium oxysporum (tentatively termed p-450dnir) was isolated by an immunoscreening method. sequence determination revealed a polypeptide of 403 amimo acid residues (mr = 44,371), which was shown to contain the full-length sequence of the fungal p-450. the amino terminus region of the predicted sequence contained neither the signal-like, hydrophobic domain that is commonly observed in microsomal p-450s nor the ...19912037602
denitrification by the fungus fusarium oxysporum and involvement of cytochrome p-450 in the respiratory nitrite reduction.from conditions for production in fusarium oxysporum of the unique nitrate/nitrite-inducible cytochrome p-450, tentatively called p-450dnir, it was expected that the fungus is capable of metabolizing nitrate dissimilatively. here we report that f. oxysporum exhibits a distinct denitrifying ability which results in the anaerobic evolution of nitrous oxide (n2o) from nitrate or nitrite. comparison of the cell growth during denitrification indicated that the dissimilatory reduction of nitrate to ni ...19912040619
phytogrowth-inhibitory and antimicrobial activities of 3,4'-dihydroxy-alpha,beta-diethylstilbene, the isomer of diethylstilbestrol.3,4'-dihydroxy-alpha,beta-diethylstilbene (i), like diethylstilbestrol (ii), showed phytogrowth-inhibitory and antimicrobial activities. first, compound i showed strong growth-inhibitory activity against the roots of two kinds of plants. the inhibitory activity of i was almost equal to that of sodium 2,4-dichlorophenoxyacetate used as a positive control. the phytogrowth-inhibitory activity of i was much higher than that of ii. next, unlike ii, i had broad antifungal spectrum against phathogenic ...19912049805
accumulation of beta-fructosidase in the cell walls of tomato roots following infection by a fungal wilt pathogen.active defense in plants is associated with marked metabolic alterations, but little is known about the exact role of the reported changes in specific activity of several enzymes in infected plant tissues. beta-fructosidase (invertase), the enzyme that converts sucrose into glucose and fructose, increases upon infection by fungi and bacteria. to understand the relationship between fungal growth and beta-fructosidase accumulation, we used an antiserum raised against a purified deglycosylated carr ...199116668461
rflp mapping of i1, a new locus in tomato conferring resistance against fusarium oxysporum f. sp. lycopersici race 1.the inheritance and linkage relationships of a gene for resistance to fusarium oxysporum f. sp. lycopersici race 1 were analyzed. an interspecific hybrid between a resistant lycopersicon pennellii and a susceptible l. esculentum was backcrossed to l. esculentum. the genotype of each backcross-1 (bc1) plant with respect to its fusarium response was determined by means of backcross-2 progeny tests. resistance was controlled by a single dominant gene, i1, which was not allelic to i, the traditional ...199124212856
time-course study of the accumulation of hydroxyproline-rich glycoproteins in root cells of susceptible and resistant tomato plants infected by fusarium oxysporum f. sp. radicis-lycopersici.the accumulation of hydroxyproline-rich glycoproteins (hrgps) in cell walls of dicotyledonous plants is thought to be involved in the defense response to pathogens. an antiserum raised against deglycosylated hrgps from melon was used for studying the subcellular localization of these glycoproteins in susceptible and resistant tomato (lycopersicon esculentum mill.) root tissues infected by fusarium oxysporum f.sp. radicis-lycopersici. a time-course of hrgp accumulation revealed that these glycopr ...199124194071
selection for fusarium wilt disease resistance from regenerants derived from leaf callus of strawberry.resistant lines of strawberry to the fungal wilt disease caused by fusarium oxysporum f. sp. fragariae were selected strawberry plants regenerated from leaf-derived callus tissues. regenerants were transplanted to a field heavily infested with this pathogen, and normally growing plants were selected as the putative resistant lines. daughter plants produced vegetatively through runner formation of the lines were similarly tested in the pathogen-infested field over an additional three generations. ...199124221538
the in vitro physiological phenotype of tomato resistance to fusarium oxysporum f. sp. lycopersici.with the aim of dissecting host-parasite interaction processes in the system lycopersicon aesculentum-fusarium oxysporum f. sp. lycopersici we have isolated plant cell mutants having single-step alterations in their defense response. a previous analysis of the physiological phenotypes of mutant cell clones suggested that recognition is the crucial event for active defence, and that polysaccharide content, fungal growth inhibition, peroxidase induction in in vitro dual culture and ion leakage ind ...199224203038
purification and some characteristics of extracellular lipase from fusarium oxysporum f. sp. lini.an extracellular lipase was isolated from a culture filtrate of fusarium oxysporum f. sp. lini suf 402 by hydrophobic chromatography. purity of the preparation was 38-fold and recovery yield was 32%. the molecular mass of the isolated enzyme was 30 kda. the lipase had a sugar chain, and the n-terminal amino acid was modified. the optimun ph at 37°c was 7.0. the enzyme had higher activity toward p-nitrophenyl esters with long and middle chain fatty acids (c8-c18) than with a short chain fatty aci ...199227280667
correlating the effect of pretreatment on the enzymatic hydrolysis of straw.avicell, alkali-treated straw cellulose (atsc), and wheat straw were ball-milled to reduce crystallinity; wheat straw was delignified by hot (120 degrees c) sodium hydroxide solutions of various concentrations. the physically and chemically pretreated cellulosic materials were hydrolyzed by the cellulases of fusarium oxysporum strain f3. enzymic hydrolysis data were fitted by the hyperbolic correlation of holtzapple, which involves two kinetic parameters, the maximum conversion (x(max)), and the ...199218600893
foret1, a reverse transcriptase-like sequence in the filamentous fungus fusarium oxysporum.we report here the isolation of foret1, a repeated dna sequence cloned from the fungal plant pathogen fusarium oxysporum. this clone exhibits a high degree of sequence similarity with the retroviral pol genes. sequences homologous to protease, reverse transcriptase, ribonuclease h are found in that order. the overall structure is homologous to the 'gypsy' class of ltr-retrotransposons. its similarity to elements present in widely different organisms may result from its horizontal transmission in ...19921380691
purification and characterization of a novel lactonohydrolase, catalyzing the hydrolysis of aldonate lactones and aromatic lactones, from fusarium oxysporum.a novel lactonohydrolase, an enzyme that catalyzes the hydrolysis of aldonate lactones to the corresponding aldonic acids, was purified 10-fold to apparent homogeneity, with a 61% overall recovery, from fusarium oxysporum aku 3702, through a purification procedure comprising deae-sephacel, octyl-sepharose cl-4b and hydroxyapatite chromatographies and crystallization. the molecular mass of the native enzyme, as estimated by high-performance gel-permeation chromatography, is 125 kda, and the subun ...19921396712
d-xylose fermentation and catabolism in fusarium oxysporum.fusarium oxysporum exhibits different fermentative capacities when grown under different aeration conditions. highest ethanol and acetic acid yield coefficients were obtained under semi-aerobic culture conditions. effect of aerobiosis on the levels of key enzymes and intracellular intermediary metabolites of pentose phosphate pathway, tricarboxylic acid cycle and glycolysis exhibited striking correlation with the fermentative character of metabolism. the results are discussed in relation to the ...19921417916
effect of pseudobactin 358 production by pseudomonas putida wcs358 on suppression of fusarium wilt of carnations by nonpathogenic fusarium oxysporum fo47.nonpathogenic fusarium oxysporum fo47b10 combined with pseudomonas putida wcs358 efficiently suppressed fusarium wilt of carnations grown in soilless culture. this suppression was significantly higher than that obtained by inoculation of either antagonistic microorganism alone. the increased suppression obtained by fo47b10 combined with wcs358 only occurred when fo47b10 was introduced at a density high enough (at least 10 times higher than that of the pathogen) to be efficient on its own. p. put ...19921444411
induction and regulation of d-xylose catabolizing enzymes in fusarium oxysporum.in fusarium oxysporum dsm 841, the requirement of oxygen was found to be associated with the induction of key d-xylose catabolizing enzymes. nadph-linked d-xylose reductase and nad-linked xylitol dehydrogenase. both enzymes were detected in cell free extracts of f. oxysporum grown in the medium containing d-xylose, l-arabinose and d-galactose as substrate. d-xylose, l-arabinose and d-galactose were also found to be substrates for nadph-linked activities. however, unlike xylitol, neither l-arabit ...19921482390
systemic ketoconazole treatment for fusarium leg ulcers.fusarium oxysporum was isolated from a large foot ulcer in an otherwise healthy 69-year-old man. although tissue invasion could not be proven histologically, systemic antifungal treatment was administered with satisfactory response. fusarium species are common soil-inhabiting organisms and plant pathogens. in humans, fusarium is considered an opportunistic agent in skin ulcers, interdigital spaces, and burned skin, but can also cause mycotic keratitis, onychomycosis, and rarely deep-seated or di ...19921500248
mechanisms of citral phototoxicity.citral, a monoterpene aldehyde synthesized by several plant genera, has been reported to exhibit antimicrobial activity. for the first time, we report that critral exhibits uv-a (315-400 nm) light enhanced oxygen-dependent toxicity against a series of escherichia coli strains differing in dna repair and catalase proficiency. those e. coli strains carrying a gene leading to catalase deficiency (katf) are particularly sensitized to inactivation by citral and uv-a treatment when compared to catalas ...19921502265
antifungal potential of binary and mixed-ligand complexes of n,2'-diphenyl acetohydroxamic acid.chelating potential of n,2'-dpaha with 3d metal ions such as cu(ii), ni(ii), zn(ii), and cd(ii) in the presence of gly and phen has been investigated. these experiments were designed to study the role of the stability of mixed-ligand complexes in the modulation of its fungicidal potential. the mixed-ligand complexes were found to be more stable than binary complexes. enhanced stability of mixed-ligand complexes of ni(ii), co(ii), zn(ii), and cd(ii) is presumably due to pi-bonding effects. in the ...19921517732
ligational behavior of n-substituted acid hydrazides towards transition metals and potentiation of their microbiocidal activity.new complexes of cu(ii), ni(ii), and co(ii) with 3-benzoyl-1-[2-n-(substituted-2'-thienyl methylmethylene/methylene)] prop-2-ene-1-oic acid hydrazides have been synthesized and characterized by elemental analysis, molecular weight determination, molar conductance, and magnetic moment and spectroscopic techniques. conductance measurements indicate the nonionic nature of the complexes. from the spectroscopic studies, it has been concluded that the n-substituted acid hydrazides act as tridentate li ...19921522414
study on the pathogenic factors of kashin-beck disease.kashin-beck disease (kbd) is a chronic osteoarthritic disease, endemic in parts of china. its etiology is unknown. selenium deficiency, high concentration of organic matter (mainly fulvic acid) in drinking water, and severe contamination of grain by fungi have been proposed environmental causes. free radicals, possible mediators between the environmental factors and origin of kbd, have been studied in this work. drinking water from kbd-affected areas contains a higher level of semiquinone radica ...19921542136
antifungal proteins from plants. purification, molecular cloning, and antifungal properties of chitinases from maize seed.we have purified two 28-kda chitinases, designated chitinase a (chit a) and chitinase b (chit b), from maize seeds to homogeneity and isolated cdna clones encoding these two enzymes using an oligonucleotide probe based on an amino acid sequence of a peptide derived from chit a. although these two enzymes share 87% homology in their amino acid sequences, which were deduced from the nucleotide sequences of the isolated cdna clones, they are significantly different in their biochemical and in vitro ...19921551872
chitinous components of the cell wall of fusarium oxysporum.the cell wall of fusarium oxysporum f. sp. lycopersici was digested with chitinase to analyze the structure of its chitinous components. in spite of a similar acetylation degree of the cell wall components to that of 25-35% acetylated chitosan, only n-acetylglucosamine disaccharide [(glcnac)2] was obtained from chitinase hydrolyzate of the fungal cell wall by cm-sephadex c-25 column chromatography, while (glcnac)2 and several types of deacetylated chitooligosaccharides were separated from that o ...19921282049
structure of beta-d-glucans from fusarium oxysporum. 19921291060
decreased immunological responses by wortmannin-containing rice culture of fusarium oxysporum and by purified wortmannin in avian species.immunological assays were performed in young chicken and duck after they had been fed wortmannin-containing culture of fusarium oxysporum or purified wortmannin for 2 weeks. the culture significantly decreased humoral response to sheep red blood cell, cell-mediated cutaneous hypersensitivity to phytohemagglutinin and phagocytic activity in isolated peritoneal exudate adherent cells, but only when the concentration was high enough to cause concurrent reduction in body weight gain and hematocrit. ...19921294627
fot1, a new family of fungal transposable elements.we report here the discovery of a family of transposable elements, which we refer to as fot1 elements, in the fungal plant pathogen fusarium oxysporum. the first element was identified as an insertion in the gene encoding nitrate reductase. it is 1928 bp long, has 44 bp inverted terminal repeats, contains a large open reading frame and is flanked by a 2 bp (ta) target site duplication. this element shares significant structural similarities with a class of transposons that includes tc1 from caen ...19921313143
saprophytic and keratinophilic fungi isolated from desert and cultivated soils continuously exposed to cement dust particles in egypt.forty soil samples collected from desert (uncultivated) and cultivated soils, exposed continuously to cement dust were screened for their content of saprophytic and keratinophilic fungi using the hair baiting technique and the dilution plate method. using the hair baiting technique, 21 genera and 31 species were collected from 20 samples of each of desert (15 genera and 21 species) and cultivated (17 genera and 24 species) soils, on sabouraud's dextrose agar. these included chrysosporium keratin ...19921325092
identification of ca2+/calmodulin-dependent protein kinase and endogenous substrate of fusarium oxysporum.ca2+/calmodulin-dependent phosphorylation and cross-reactivity between anti-rat brain ca2+/calmodulin-dependent protein kinase ii (camk) antibody and partially purified camk from fusarium oxysporum were detected in the component of high-molecular mass (m(r) greater than 100,000). in vitro, ca2+/cam-dependent phosphorylation of only a 16-kda protein was detected. the 16-kda protein was localized in the membrane fraction. amino acid sequence of one of the peptides derived from partial hydrolysis o ...19921325938
glucose-induced activation of the plasma membrane h(+)-atpase in fusarium oxysporum.addition of glucose and other sugars to derepressed cells of the fungus fusarium oxysporum var. lini triggered activation of the plasma membrane h(+)-atpase within 5 min. glucose was the best activator while galactose and lactose had a lesser effect. the activation was not prevented by previous addition of cycloheximide and it was fully reversible when the glucose was removed. the activation process in vivo also caused changes in the kinetic properties of the enzyme. the non-activated enzyme had ...19921326595
mutational analysis of genes determining antagonism of alcaligenes sp. strain mfa1 against the phytopathogenic fungus fusarium oxysporum.alcaligenes sp. strain mfa1 inhibits microconidial germination and germination-tube elongation of fusarium oxysporum f.sp. dianthi and reduces the severity of fusarium wilt of carnation, presumably as a result of its production of a siderophore (g.y. yuen and m.n. schroth. 1986. phytopathology, 76:171-176). derivative strains of mfa1, deficient in antagonism against f. oxysporum and in iron-limited growth, were obtained by tn5 mutagenesis. the presence of a single tn5 insertion in the genomic dn ...19921327482
a fatal infection due to fusarium oxysporum in a child with wilms' tumour. case report and review of the literature.fusarium oxysporum was isolated twice from the blood culture of a 5-year-old boy with inoperable wilms' tumour (stage iv) 4 weeks after a cytoreductive therapy with actinomycin d, vincristine and adriamycin. the child died 3 weeks after the first isolation of the fungus with signs of hepatic failure and consumptive coagulopathy. the importance of infection with fusarium spp. in immunocompromised neutropenic patients and their pathogenetic role are discussed in the view of the literature.19921335549
correlation of genetic and physical structure in the region surrounding the i2 fusarium oxysporum resistance locus in tomato.the dominant gene i2 confers on tomato (lycopersicon esculentum) resistance against the fungus fusarium oxysporum f. sp. lycopersici race 2. a restriction fragment length polymorphism (rflp) marker, tg105, has recently been found to be tightly linked to i2. the potential for cloning this gene by a reverse genetics approach prompted us to describe in both genetic and physical detail the region surrounding the i2 locus on chromosome 11. we have analyzed patterns of segregation of rflp markers on c ...19921346546
isolation and characterization of a 22 kda protein with antifungal properties from maize seeds.we have purified a 22 kda protein from maize seeds to homogeneity by ammonium sulfate precipitation, chitin extraction and mono-s column chromatography. the purified protein inhibited the growth of the agronomically important pathogens of potato wilt (fusarium oxysporum) and tomato early blight (alternaria solani). sequence analysis of the purified protein showed that it has 52% homology with the sweet protein thaumatin (edens, l., hselinga, l., klok, r., ledeboer, a. m., maat, j., toonen, m. y. ...19921731773
cloning and expression of a chitinase gene from the hyperparasitic fungus aphanocladium album.recombinant clones from a cdna library of an aphanocladium album chitinase-overproducing mutant strain were isolated by screening with antiserum against a 39 kda chitinase purified from this hyperparasitic fungus. analysis of the isolated positive clones indicated that most of them carried the same cdna. a cdna from this group was used as a hybridization probe to isolate an 8 kb dna fragment from a genomic library of the wild-type strain. the chitinase 1 gene was mapped to this fragment by two i ...19921735126
stem nematode-fusarium wilt complex in alfalfa as related to irrigation management at harvest time.a high moisture level in the top 10 cm of soil at time of cutting of alfalfa increased the incidence of plant mortality and fusarium wilt in soil infested with ditylenchus dipsaci and fusarium oxysporum f. sp. medicaginis in greenhouse and field microplot studies. ranger alfalfa, susceptible to both d. dipsaci and f. oxysporum f. sp. medicaginis, was less persistent than moapa 69 (nematode susceptible and fusarium wilt resistant) and lahontan alfalfa (nematode resistant with low fusarium wilt re ...199219283002
predisposition of broadleaf tobacco to fusarium wilt by early infection with globodera tabacum tabacum or meloidogyne hapla.in greenhouse experiments, broadleaf tobacco plants were inoculated with tobacco cyst (globodera tabacum tabacum) or root-knot (meloidogyne hapla) nematodes 3, 2, or 1 week before or at the same time as fusarium oxysporum. plants infected with nematodes prior to fungal inoculation had greater fusarium wilt incidence and severity than those simultaneously inoculated. g. t. tabacum increased wilt incidence and severity more than did m. hapla. mechanical root wounding within 1 week of f. oxysporum ...199219283018
evaluation of hot water treatments for management of ditylenchus dipsaci and fungi in daffodil bulbs.treatment of daffodil (narcissus pseudonarcissus) bulbs in a 0.37% formaldehyde water solution at 44 c for 240 minutes is a standard practice in california for management of the stem and bulb nematode, ditylenchus dipsaci. recent concern over the safety of formaldehyde and growers' requests for a shorter treatment time prompted a reevaluation of the procedure. the time (y, in minutes) required to raise the temperature at the bulb center from 25 to 44 c was related to bulb circumference (x, in cm ...199319279827
the gus gene fusion system (escherichia coli beta-d-glucuronidase gene), a useful tool in studies of root colonization by fusarium oxysporum.the plant-pathogenic fungus fusarium oxysporum was successfully transformed with the beta-d-glucuronidase gene from escherichia coli (gusa) (gus system) in combination with the gene for nitrate reductase (niad) as the selectable marker. the frequency of cotransformation, as determined by gus expression on plates containing medium supplemented with 5-bromo-4-chloro-3-indolyl glucuronide (gus+), was very high (up to 75%). southern hybridization analyses of gus+ transformants revealed that single o ...19938328800
the sequence and x-ray structure of the trypsin from fusarium oxysporum.the trypsin from fusarium oxysporum is equally homologous to trypsins from streptomyces griseus, streptomyces erythraeus and to bovine trypsin. a dfp (diisopropylfluorophosphate) inhibited form of the enzyme has been crystallized from 1.4 m na2so4, buffered with citrate at ph 5.0-5.5. the crystals belong to space group p2(1) with cell parameters a = 33.43 a, b = 67.65 a, c = 39.85 a and beta = 107.6 degrees. there is one protein molecule in the asymmetric unit. x-ray diffraction data to a resolu ...19938332590
synthesis and antifungal activity of medicagenic acid saponins on plant pathogens: modification of the saccharide moiety and the 23 alpha substitution.the study of structure-antifungal activity relationships of medicagenic acid saponins was widened to include synthetic glycosides of mannose, galactose, cellobiose, and lactose as well as a 23 alpha-hydroxymethyl analog of medicagenic acid, namely, methyl 2 beta,3 beta-dihydroxy-23 alpha-hydroxymethyl-delta (12)-oleanene-28 beta-carboxylate, against sclerotium rolfsii, rhizoctonia solani, trichoderma viride, aspergillus niger, and fusarium oxysporum. the native glucose-containing saponin was a m ...19938339299
synthesis and fungitoxicity of some pyrimidine derivatives.a series of 12 pyrimidine derivatives were prepared and tested in vitro against growth, sporulation and nucleic acid content of fusarium oxysporum f. sp. lycopersici and helminthosporium oryzae. introduction of a thiazole ring together with two aryl groups into 2-aminopyrimidine brought about drastic toxicity for both fungi. pyrimidine derivatives with aryl groups alone were less toxic. nitro groups were found to enhance the toxicity of the pyrimidine derivatives especially when substituted in t ...19938365695
the nia gene of fusarium oxysporum: isolation, sequence and development of a homologous transformation system.the fusarium oxysporum gene nia, encoding nitrate reductase (nr), was isolated from a cosmid library by direct complementation of an f. oxysporum nia- mutant. the gene specifies a protein of 905 amino acids and contains a 57-bp intron. comparison of the deduced aa sequence with nr of other fungi revealed a high degree of similarity and conservation in the intron position. the cloned nia made it possible to develop the first homologous transformation system for this fungus. transformation frequen ...19938370541
new cause for false-positive results with the pastorex aspergillus antigen latex agglutination test.the pastorex aspergillus antigen test for detection of aspergillus galactomannan antigen in the sera of patients with invasive aspergillosis is used in many clinical laboratories. a serum sample contaminated with penicillium chrysogenum gave a strongly positive reaction (1:128) which was heat stable, was not eliminated by pronase treatment, and was not detected by a normal rabbit globulin control. this observation was shown to be due to cross-reactions of the monoclonal antibody eb-a2 used by th ...19938408572
cytochrome p-450 55a1 (p-450dnir) acts as nitric oxide reductase employing nadh as the direct electron donor.cytochrome p-450dnir (p-450dnir), involved in the fungal denitrification by fusarium oxysporum, was purified to homogeneity. the cytochrome p-450 (p-450) exhibited a potent nitric oxide (no) reductase activity to form nitrous oxide (n2o) employing nadh but not nadph as the sole effective electron donor. the apparent maximum turnover rate against no was estimated as high as 31,500 min-1. the stoichiometry of the reaction between no:nadh:n2o was 2:1:1. the reaction required neither an artificial e ...19938463342
a colorimetric assay for penicillin-v amidase.the hydrolysis of penicillin-v to phenoxyacetic acid and 6-aminopenicillanic acid by the fungal enzyme penicillin-v amidase is of industrial importance since the 6-aminopenicillanic acid produced is an intermediate for semisynthetic penicillins. a rapid colorimetric assay of penicillin-v amidase was developed which uses 2-nitro-5-(phenoxyacetamido)-benzoic acid as a substrate. the released chromophore, 2-amino-5-nitrobenzoic acid, was detected at 405 nm. using penicillin-v amidase from the fungu ...19938470805
production and some properties of fibrinolytic enzymes from fusarium oxysporum n.r.c.1.a mixture of yeast extract and peptone in the culture medium was the most favourable for the production of active fibrinolytic enzyme by fusarium oxysporum n.r.c.1. potassium dihydrogen phosphate had stimulating effect, while glucose, sucrose, lactose, ribose and soluble starch had adverse effect on enzyme productivity. the optimum of the fibrinolytic enzyme activity was at ph 7.0.19938480452
purification and properties of two fibrinolytic enzymes from fusarium oxysporum n.r.c.1.the crude fibrinolytic enzyme preparation from fusarium oxysporum n.r.c.1 was purified into two enzymes by ammonium sulphate precipitation followed by chromatography on sephadex g-100 and deae-cellulose. both fibrinolytic enzymes were more active on human than on bovine fibrin. the activity of the "major" enzyme component on human fibrin was 72-fold that of the "minor" enzyme component. both enzymes had the same temperature (37 degrees c) and ph (6.98) optima. the "minor" enzyme component was mo ...19938480453
antifungal properties of lectin and new chitinases from potato tubers.we have purified from potato tubers, the lectin sta devoid of chitinase activity and two chitinases devoid of lectin activity. both enzymes are 16 kda glycoproteins, and probably belong to a new family of plant chitinases. the respective antifungal properties of lectin and chitinases were studied by following their effects against early developmental stages of fusarium oxysporum, a fungal potato pathogen. here we demonstrate that: (1) lectin does not inhibit mycelial growth but irreversibly inhi ...19938044703
molds in onychomycosis.onychomycosis is a major cause of nail dystrophy. the causative organisms in onychomycosis are dermatophytes, candida and molds. a variety of molds have been isolated from nails.19938125689
antagonistic effect of nonpathogenic fusarium oxysporum fo47 and pseudobactin 358 upon pathogenic fusarium oxysporum f. sp. dianthi.pseudobactin production by pseudomonas putida wcs358 significantly improves biological control of fusarium wilt caused by nonpathogenic fusarium oxysporum fo47b10 (p. lemanceau, p. a. h. m. bakker, w. j. de kogel, c. alabouvette, and b. schippers, appl. environ. microbiol. 58:2978-2982, 1992). the antagonistic effect of fo47b10 and purified pseudobactin 358 was studied by using an in vitro bioassay. this bioassay allows studies on interactions among nonpathogenic f. oxysporum fo47b10, pathogenic ...199316348860
utilization of lactic acid by fusarium oxysporum var. lini: regulation of transport and metabolism.lactic acid was transported in fusarium oxysporum var. lini atcc 10960 by a saturable transport system that had a half-saturation constant of 56.6 +/- 7.5 mum and a maximum velocity of 0.61 +/- 0.10 mmol h g (dry weight) at 26 degrees c and ph 5.0. this transport system was inducible and was not expressed in the presence of a repressing substrate. evidence is presented that the anionic form lactate was taken up by the cells. propionic, acetic, pyruvic, and bromoacetic acids but not succinic acid ...199416349143
evolutionary divergence and conservation of trypsin.the trypsin sequences currently available in the data banks have been collected and aligned using first the amino acid sequence homology and, subsequently, the superposed crystal structures of trypsins from the cow, the bacterium streptomyces griseus and the fungus fusarium oxysporum. the phylogenetic tree constructed according to this multiple alignment is consistent with a continuous evolutionary divergence of trypsin from a common ancestor of both prokaryotes and eukaryotes. comparison of cry ...19948140095
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