TitleAbstractYear(sorted ascending)
general characterization of noncommercial microbial lipases in hydrolytic and synthetic reactions.fourteen noncommercial preparations of microbial lipases were investigated with respect to their catalytic activity for hydrolysis and synthesis of ester bonds. six of the lipases were derived from microorganisms that have not previously been described as lipase producers, and another four were characterized for the first time. the synthetic reactions were carried out in two solvents of different polarities (n-heptane and acetone) using a series of fatty acids and primary and secondary alcohols ...200515767695
organophosphorus derivatives containing piperazine dithiosemicarbazones as chemotherapeutants against fungal pathogens of sugarcane.five novel organophosphorus derivatives have been synthesized by the reactions of o,o-diethylchlorophosphate with piperazine dithiosemicarbazones. the derivatives have been characterized on the basis of analyses and spectral (ir, 1h nmr) data. fungicidal activities of these derivatives against colletotrichum falcatum, fusarium oxysporum, and curvularia pallescence have been evaluated. the screening results have been correlated with the structural features of the tested compounds. organophosphoru ...200515769154
synthesis and fungicidal activity of novel 2-oxocycloalkylsulfonylureas.a series of 2-oxocycloalkylsulfonylureas (2) have been synthesized in a six-step, three-pot reaction sequence from readily available cyclododecanone, cycloheptanone, and cyclohexanone. their structures were confirmed by ir, 1h nmr, and elemental analysis. the bioassay indicated that some of them possess certain fungicidal activity against gibberella zeae petch. in general, compounds containing a 12-membered ring (2a) are more active than those containing a 6- or 7-membered ring (2b, 2c). in the ...200515769157
fungal populations and mycotoxins in silage in assiut and sohag governorates in egypt, with a special reference to characteristic aspergilli toxins.forty silage samples were collected from assiut and sohag governorates in egypt to measure the presence of fungal population in silage. forty-three species and 2 species varieties belonging to 17 genera were isolated using glucose czapek's and sabouraud's dextrose agar media at 28 degrees c. the most prevalent genera were aspergillus (57.5 and 100 of the samples), penicillium (100 and 55%) on the two mentioned media, respectively. also, fusarium oxysporum and gibberella fujikurori were recovered ...200515770455
molecular cloning and characterization of cdna of penaeidin-like antimicrobial peptide from tiger shrimp (penaeus monodon).we report the molecular cloning and characterization of a penaeidin-like antimicrobial peptide (amp) complementary dna from the hemocytes of tiger shrimp (penaeus monodoni). a tiger shrimp amp cdna containing the entire coding region of the peptide, determined by rapid amplification of cdna 5' and 3' ends and polymerase chain reaction of the messenger rna isolated from hemocytes of the shrimp, is 683 bp in length with an open reading frame of 222 bp. the deduced amino acid sequence of this antim ...200515776314
[effect of aeration on composting of date palm residues contaminated with fusarium oxysporum f.sp. albedinis].composting of date palm (phoenix dactylifera l.) residues contaminated with fusarium f.sp oxysporum albedinis, causal agent of the vascular wilt (bayoud) of the date palm, has been achieved. the effect of the aeration of the piles by manual turning has been studied. the maintenance of an adequate humidity of 60%-70%, necessary to the good progress of the composting process, required the contribution of 11.4 l of water/kg of the dried residues. the evolution of the temperatures in the three piles ...200515782236
the pseudomonas chlororaphis pcl1391 sigma regulator psra represses the production of the antifungal metabolite phenazine-1-carboxamide.the rhizobacterium pseudomonas chlororaphis pcl1391 produces the antifungal metabolite phenazine-1-carboxamide (pcn), which is a crucial trait in its competition with the phytopathogenic fungus fusarium oxysporum f. sp. radicis-lycopersici in the rhizosphere. the expression of the pcn biosynthetic gene cluster in pcl1391 is population density-dependent and is regulated by the quorum-sensing genes phzi and phzr via synthesis of the autoinducer n-hexanoyl-l-homoserine lactone (c6-hsl). here, we de ...200515782638
morning glory systemically accumulates scopoletin and scopolin after interaction with fusarium isolate of non-pathogenic fusarium, fusarium oxysporum 101-2 (npf), induces resistance in the cuttings of morning glory against fusarium wilt caused by f. oxysporum f. sp. batatas o-17 (pf). the effect of npf on phenylpropanoid metabolism in morning glory cuttings was studied. it was found that morning glory tissues responded to treatment with npf bud-cell suspension (108 bud-cells/ml) with the activation of phenylalanine ammonia-lyase (pal). pal activity was induced faster and greater in the ...200515787250
isolation, identification and antifungal susceptibility of lemon pathogenic and non pathogenic fungi.numerous species of filamentous fungi were isolated from lemon on different plantations in the province of tucuman, argentina. the techniques suggested by the subcommittee of antifungal susceptibility of the national committee for clinical laboratory standards, (usa) were adapted. the effect of three different concentrations of the fungicides imazalil, guazatine, sopp and thiabendazole on the fungi fusarium oxysporum, fusarium moniliforme, aspergillus niger, aspergillus flavus, aspergillus clava ...200515813686
novel quorum-sensing-controlled genes in erwinia carotovora subsp. carotovora: identification of a fungal elicitor homologue in a soft-rotting new genes controlled by the quorum-sensing signal molecule n-(3-oxohexanoyl)-l-homoserine lactone (ohhl) have been identified in erwinia carotovora subsp. carotovora. using tnphoa as a mutagen, we enriched for mutants defective in proteins that could play a role in the interaction between e. carotovora subsp. carotovora and its plant hosts, and identified nipecc and its counterpart in e. carotovora subsp. atroseptica. these are members of a growing family of proteins related to nep1 from f ...200515828686
efficiency of blood culture bottles for the fungal sterility testing of corneal organ culture media.the consequences of fungal contamination of an organ cultured cornea, though exceptional, are often disastrous for the recipient. consequently, eye banks often quarantine corneas for 10 days or more before passing them for grafting. this period, though detrimental to the endothelial cell density of the delivered cornea, is necessary to detect contamination using conventional microbiological methods. the authors previously validated the use of a pair of aerobic and anaerobic blood bottles for sen ...200515834090
strain-specific and recessive qtls involved in the control of partial resistance to fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon.fusarium oxysporum f. sp. melonis (fom) causes serious economic losses in melon (cucumis melo l.). two dominant resistance genes have been identified, fom-1 and fom-2, which provide resistance to races 0 and 2 and races 0 and 1, respectively, however fom race 1.2 overcomes these resistance genes. a partial resistance to fom race 1.2 that has been found in some far east accessions is under polygenic control. a genetic map of melon was constructed to tag fom race 1.2 resistance with dna markers on ...200515834544
purification and characterization of a chitinase-like antifungal protein from black turtle bean with stimulatory effect on nitric oxide production by antifungal protein was isolated from black turtle bean, phaseolus vulgaris cv. "black turtle". the purified protein displayed an n-terminal amino acid sequence with 60-80% homology to chitinases. the isolated protein specifically inhibited two species of fungi, namely fusarium oxysporum and mycosphaerella arachidicola , among several phytopathogenic fungi tested. its antifungal activity was retained after incubation at 60 degrees c for 15 minutes, diminished after exposure to 80 degrees c, an ...200515843143
synthesis and characterization of novel 6-fluoro-4-piperidinyl-1,2-benzisoxazole amides and 6-fluoro-chroman-2-carboxamides: antimicrobial studies.novel derivatives of 6-fluoro-4-piperidinyl-1,2-benzisoxazole amides 4(i-vi) were obtained by the condensation of different acid chlorides with 6-fluoro-3-piperidin-4yl-benzo[d]isoxazole. also, 6-fluoro-chroman-2-carboxamides 6(i-iii) were synthesized by using nebulic acid chloride with different amines in presence of triethylamine as acid scavenger and dichloroethane as solvent. the synthesized compounds were characterized by ir, 1h nmr, and chn analysis. these molecules were evaluated for thei ...200515846867
antifungal properties of surangin b, a coumarin from mammea longifolia.the natural product electron transport inhibitor surangin b was examined for its ability to inhibit in vitro mycelial growth and spore germination in several species of fungi. as an inhibitor of mycelial growth, surangin b showed strongest activity against rhizoctonia solani (ic50 = 3.8 microm) and botrytis cinerea (ic50 = 11.2 microm). inhibitory effects were less pronounced in alternaria dauci, fusarium oxysporum and penicillium sp. (ic50 values > 30 microm) and absent in trichoderma harzianum ...200515856416
a dual selection based, targeted gene replacement tool for magnaporthe grisea and fusarium oxysporum.rapid progress in fungal genome sequencing presents many new opportunities for functional genomic analysis of fungal biology through the systematic mutagenesis of the genes identified through sequencing. however, the lack of efficient tools for targeted gene replacement is a limiting factor for fungal functional genomics, as it often necessitates the screening of a large number of transformants to identify the desired mutant. we developed an efficient method of gene replacement and evaluated fac ...200515893252
fungal endophytes in five medicinal plant species from kudremukh range, western ghats of india.eighteen species of endophytic fungi were isolated from bark, stem and leaf segments of five medicinal plant species growing within the kudremukh range in the western ghats of india. the dominant endophytic fungal species isolated from these plant species were curvularia clavata, c. lunata, c. pallescens and fusarium oxysporum. the highest species richness as well as frequency of colonization of endophytic fungi was found in the leaf segments, rather than the stem and bark segments, of the host ...200515900544
purification and characterization of a novel peptide with antifungal activity from bothrops jararaca venom.different peptides have been isolated from a wide range of animal species. it is has become increasingly clear that due to the development of antibiotic-resistant microbes, antibacterial and antifungal peptides have attracted the attention in recent years, in order to find new therapeutic agents. in this work, a novel peptide with high inhibitory activity against fungi growth have been isolated from the venom of the brazilian snake bothrops jararaca. a sephacryl s-100 gel filtration column was e ...200515904677
sucrose-induced lupine defense against fusarium oxysporum. sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to fusarium oxysporum.defense responses to inoculation with fusarium oxysporum schlecht f. sp. lupini were studied in embryo axes of lupinus luteus l. cv. polo cultured on a medium with sucrose (60 mm) or without it. exogenous sucrose caused a marked endogenous increase in concentrations of sucrose, glucose and fructose in embryo axes. in axes cultured with sucrose, high performance liquid chromatography (hplc) revealed generally higher levels of isoflavone glycosides (particularly until 48 h of culture) and free agl ...200515907688
suppression of meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria.four rhizobacteria selected out of over 500 isolates from rhizosphere of the vegetables in china were further studied for suppression of the root-knot nematode and soil-borne fungal pathogens in laboratory and greenhouse in belgium. they were identified as brevibacillus brevis or bacillus subtilis by biolog test and partial 16s rdna sequence comparison. they not only inhibited the radial growth of the root-infecting fungi rhizoctonia solani sx-6, pythium aphanidermatum zjp-1 and fusarium oxyspor ...200515909333
the potential efficiency of irrigation management and propargyl bromide in controlling three soil pests: tylenchulus semipenetrans, fusarium oxysporum and echinochloa crus-galli.propargyl bromide (3-bromopropyne, 3bp) is a potential alternative for methyl bromide. little information is available about its efficiency in controlling pests. the purpose of this paper is to estimate the 3bp dose required for killing three pests and to compare the efficiency of water management approaches to that of fumigation. the pests, fusarium oxysporum schlecht (fungus), echinochloa crus-galli (l) beauv (grass) and tylenchulus semipenetrans cobb (nematode) were exposed to different 3bp c ...200515912563
the rim101/pacc homologue from the basidiomycete ustilago maydis is functional in multiple ph-sensitive phenomena.a homologue of the gene encoding the transcription factor rim101 (pacc), involved in ph signal transduction in fungi, was identified in the pathogenic basidiomycete ustilago maydis. the gene (rim101) encodes a protein of 827 amino acid residues, which shows highest similarity to pacc proteins from fusarium oxysporum and aspergillus niger. the gene had the capacity to restore protease activity to rim101 mutants from yarrowia lipolytica, confirming its homologous function, and was expressed at bot ...200515947192
extraction of functional motion in trypsin crystal structures.the analysis of anisotropic atomic displacement parameters for the direct extraction of functionally relevant motion from x-ray crystal structures of fusarium oxysporum trypsin is presented. several atomic resolution structures complexed with inhibitors or substrates and determined at different ph values and temperatures were investigated. the analysis revealed a breathing-like molecular motion conserved across trypsin structures from two organisms and three different crystal forms. directional ...200516041079
visualization of interactions between a pathogenic and a beneficial fusarium strain during biocontrol of tomato foot and root rot.the soilborne fungus fusarium oxysporum f. sp. radicis-lycopersici causes tomato foot and root rot (tfrr), which can be controlled by the addition of the nonpathogenic fungus f. oxysporum fo47 to the soil. to improve our understanding of the interactions between the two fusarium strains on tomato roots during biocontrol, the fungi were labeled using different autofluorescent proteins as markers and subsequently visualized using confocal laser scanning microscopy. the results were as follows. i) ...200516042017
heavy-metal remediation by a fungus as a means of production of lead and cadmium carbonate crystals.we show here that reaction of the fungus, fusarium oxysporum, with the aqueous heavy-metal ions pb2+ and cd2+ results in the one-step formation of the corresponding metal carbonates. the metal carbonates are formed by reaction of the heavy-metal ions with co2 produced by the fungus during metabolism and thus provide a completely biological method for production of crystals of metal carbonates. the pbco3 and cdco3 crystals thus produced have interesting morphologies that are shown to arise becaus ...200516042445
taking the fungal highway: mobilization of pollutant-degrading bacteria by fungi.the capacity of fungi to serve as vectors for the dispersion of pollutant-degrading bacteria was analyzed in laboratory model systems mimicking water-saturated (agar surfaces) and unsaturated soil environments (glass-bead-filled columns). two common soil fungi (fusarium oxysporum and rhexocercosporidium sp.) forming hydrophilic and hydrophobic mycelia, respectively, and three polycyclic aromatic hydrocarbon degrading bacteria (achromobacter sp. sk1, mycobacterium frederiksbergense lb501tg, and s ...200516047804
studies on the management of root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex of green gram, vigna radiata cv ml-1108.studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (azadirachta indica) seed powder at 50 mg/kg soil, green mould (trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex on green gram, vigna radiata cv ml-1108. all the treatments significantly improved ...200516052706
biodegradation and detoxification of organophosphate insecticide, malathion by fusarium oxysporum f. sp. pisi cutinase.efficiencies of two lypolytic enzymes (fungal cutinase and yeast esterase) in malathion degradation were investigated. surprisingly, degradation rate of malathion by fungal cutinase was very high, i.e. almost 60% of initial malathion (500 mg l(-1)) was decomposed within 0.5 h, and nearly 50% of the degraded malathion disappeared within initial 15 min. with the yeast esterase, despite the same concentration, more than 65% of malathion remained even after 2-day treatment. during enzymatic degradat ...200516054903
biodegradation of dipropyl phthalate and toxicity of its degradation products: a comparison of fusarium oxysporum f. sp. pisi cutinase and candida cylindracea esterase.the efficiency of two lypolytic enzymes (fungal cutinase, yeast esterase) in the degradation of dipropyl phthalate (dprp) was investigated. the dprp-degradation rate of fungal cutinase was surprisingly high, i.e., almost 70% of the initial dprp (500 mg/l) was decomposed within 2.5 h and nearly 50% of the degraded dprp disappeared within the initial 15 min. with the yeast esterase, despite the same concentration, more than 90% of the dprp remained even after 3 days of treatment. during the enzyma ...200516059706
influence of fusaric acid on phenazine-1-carboxamide synthesis and gene expression of pseudomonas chlororaphis strain pcl1391.production of the antifungal metabolite phenazine-1-carboxamide (pcn) by pseudomonas chlororaphis strain pcl1391 is essential for the suppression of tomato foot and root rot caused by the soil-borne fungus f. oxysporum f. sp. radicis-lycopersici. the authors have shown previously that fusaric acid (fa), a phytotoxin produced by fusarium oxysporum, represses the production of pcn and of the quorum-sensing signal n-hexanoyl-l-homoserine lactone (c(6)-hsl). here they report that pcn repression by f ...200516079356
frp1 is a fusarium oxysporum f-box protein required for pathogenicity on tomato.fusarium oxysporum f. sp. lycopersici is the causal agent of tomato wilt disease. in order to identify genes involved in its pathogenicity, we performed insertional mutagenesis. mutant n40 had lost its pathogenicity completely, when tested in bioassays with tomato seedlings. molecular characterization of mutant n40 revealed that the plasmid insertion had occurred in a gene that codes for a 60.2 kda protein containing an f-box motif. the gene was therefore designated as frp1 (f-box protein requir ...200516091043
[cloning and expression of fusarium moniliforme cgmcc 0536 d-lactonohydrolase gene in escherichia coli].the total cdna obtained through reverse transcription of f. oxysporum cgmcc 0536 mrna used as template, a fragment about 1.5kb was amplied with oligo(dt)15 primer and a gene specific primer designed on the base of the sequence of both nh2-terminus and the cdna sequence encoding d-lactonohydrolase of fusarium oxysporum reported on the ncbi, then the fragment was cloned to the pmd18-t vector and sequenced. the sequence encoding d-lactonohydrolase of f. moniliforme cgmcc 0536 shows a high homology ...200516108362
[population fluctuation of main pathogens and their antagonistic bacteria in cucumber rhizosphere].the investigation on the population fluctuation of main pathogens pythium spp. and fusarium oxysporum f. cucumerinum in the rhizosphere of four cucumber varieties showed that there was a significant difference in population number of pathogens for different cucumber varieties and for different growth stages of the same variety, which was in accordance with the regular disease incidence pattern. the population number of pythium spp. was significantly higher at cucumber creeping stage than at its ...200516110670
the transcription factor ataf2 represses the expression of pathogenesis-related genes in arabidopsis.ataf2, a member of the plant-specific nac-domain transcription factor family, is highly induced in leaves at a wound site and is responsive to the wound-related phytohormones methyl jasmonate and salicylic acid, but not to abscisic acid. overexpression of ataf2 leads to an increased biomass and yellowing of the leaves, but there is no obvious phenotype in two independent ataf2 t-dna insertion lines. at the transcriptome level, ataf2 overexpression resulted in repression of a number of pathogenes ...200516115070
toxin-based in-vitro selection and its potential application to date palm for resistance to the bayoud fusarium palm (phoenix dactylifera l.) is qualified as a 'tree' of great ecological and socio-economical importance in desert oases. unfortunately, it is being decimated, especially in morocco and algeria, by a fusariosis wilt called bayoud and caused by fusarium oxysporum f. sp. albedinis (fao). controlling this disease requires the implementation of an integrated management program. breeding for resistance is one of the most promising component strategies of this program. few naturally resistant c ...200516125651
an antifungal protein from ginger rhizomes.there are very few reports on antifungal proteins from rhizomes and there is none from the family of zingiberaceae. an antifungal protein with a novel n-terminal sequence was isolated from ginger rhizomes utilizing a protocol that involved ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, and fast protein liquid chromatography on superdex 75. the protein was unadsorbed on deae-cellulose and adsorbed on affi-gel blue gel. it exhibited an apparent molecul ...200516125680
force calculations in automated docking: enzyme-substrate interactions in fusarium oxysporum cel7b.autodock is a small-molecule docking program that uses an energy function to score docked ligands. here autodock's grid-based method for energy evaluation was exploited to evaluate the force exerted by fusarium oxysporum cel7b on the atoms of docked cellooligosaccharides and a thiooligosaccharide substrate analog. coupled with the interaction energies evaluated for each docked ligand, these forces give insight into the dynamics of the ligand in the active site, and help to elucidate the relative ...200516138313
a novel homodimeric lectin from astragalus mongholicus with antifungal activity.a novel lectin (amml) was isolated from a chinese herb, i.e., the roots of astragalus mongholicus, using a combination of ammonium sulfate fraction and ion exchange chromatographies. the molecular mass of intact amml was determined to be 66,396 da by maldi-tof mass spectrometry and 61.8 kda by gel filtration, respectively. amml was a dimeric protein composed of two identical subunits each with a molecular mass of 29.6 kda. the lectin was a glycoprotein with a neutral carbohydrate content of 19.6 ...200516140255
generation and characterization of reduced virulence fusarium oxysporum f. sp. lycopersici mutants through plasmid-vector insertion.pathogenicity-impaired mutants, b02 and h15, of fusarium oxysporum f. sp. lycorpersici (fol) were obtained using restriction enzyme-mediated integration. disease severities of fusarium wilt caused by these mutants were significantly reduced, and their disease development rates were correlated with their colonization rates in tomato vessels. both b02 and h15 produced significantly smaller amounts of extracellular proteins as well as fusaric acid than the wild-type. southern blot analyses suggeste ...200516160771
immunoglobulin g antibodies against environmental moulds in a norwegian healthy population shows a bimodal distribution for aspergillus versicolor.immunoglobulin g (igg) antibodies against moulds related to indoor dampness problems are used as biomarkers to indicate exposure. in the present study, we evaluated the frequency of mould exposure in an adult healthy population by examining levels of mould-specific igg antibodies in norwegian blood donors. using enzyme-linked immunosorbent assay, 106 blood donor sera were analyzed for igg antibodies to aspergillus versicolor, penicillium chrysogenum, cladosporium herbarum, stachybotrys chartarum ...200516179015
repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of arabidopsis transcription factor gene identify transcription factors (tfs) involved in jasmonate (ja) signaling and plant defense, we screened 1,534 arabidopsis (arabidopsis thaliana) tfs by real-time quantitative reverse transcription-pcr for their altered transcript at 6 h following either methyl ja treatment or inoculation with the incompatible pathogen alternaria brassicicola. we identified 134 tfs that showed a significant change in expression, including many apetala2/ethylene response factor (ap2/erf), myb, wrky, and nac tf ...200516183832
sesquin, a potent defensin-like antimicrobial peptide from ground beans with inhibitory activities toward tumor cells and hiv-1 reverse antifungal peptide with a molecular mass around 7 kda and an n-terminal sequence highly homologous to defensin was isolated from ground beans (vigna sesquipedalis cv. 'ground bean'). the peptide was adsorbed on affi-gel blue gel and on mono s. it exerted an antifungal action on botrytis cinerea, fusarium oxysporum and mycosphaerella arachidicola; and an antibacterial action on escherichia coli b, proteus vulgaris, mycobacterium phlei and bacillus megaterium. the antimicrobial activity was inh ...200515949629
effects of interleukin-15 on antifungal responses of human polymorphonuclear leukocytes against fusarium spp. and scedosporium spp.fusarium spp. and scedosporium spp. have emerged as important fungal pathogens that are frequently resistant to antifungal compounds. we investigated the effects of human interleukin-15 (il-15) on human polymorphonuclear leukocyte (pmnl) activity against fusarium solani and fusarium oxysporum as well as scedosporium prolificans and scedosporium apiospermum. il-15 (100 ng/ml) significantly enhanced pmnl-induced hyphal damage of both fusarium spp. and s. prolificans after incubation for 22 h (p < ...200515935692
isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots.a total of 131 endophytic actinomycete strains were successfully isolated from surface-sterilized banana roots. these isolates belonged to streptomyces (n=99), streptoverticillium (n=28), and streptosporangium (n=2) spp. the remaining 2 isolates were not identified. about 18.3% of the isolates inhibited the growth of pathogenic fusarium oxysporum f. sp. cubense on banana tissue extract medium. the most frequently isolated streptomyces sp. strain s96 was similar to streptomyces griseorubiginosus. ...200515935565
practical resolution system for dl-pantoyl lactone using the lactonase from fusarium oxysporum.we developed an enzymatic resolution system for dl-pantoyl lactone that uses immobilized mycelia of fusarium oxysporum, which produce a lactone-hydrolyzing enzyme (lactonase). the lactonase catalyzes the stereospecific hydrolysis of d-pantoyl lactone. one hundred eighty repeated batch reactions (total reaction time, 3780 h) were made with mycelia entrapped in calcium alginate gels as the catalyst, in the presence of 90 mm cacl2. with a 300 gl(-1)dl-pantoyl lactone solution as the substrate, the ...200515935504
extracellular xylanases from two pathogenic races of fusarium oxysporum f. sp. ciceris: enzyme production in culture and purification and characterization of a major isoform as an alkaline endo-beta-(1,4)-xylanase of low molecular weight.fusarium oxysporum f. sp. ciceris, the causal agent of fusarium wilt of chickpea, comprises eight pathogenic races and two pathotypes. races 0 and 5, representative of the least virulent yellowing pathotype and the most virulent wilt pathotype, respectively, produced extracellular xylanases when grown on minimal medium supplemented with either 1% commercial birchwood xylan or 0.3% chickpea cell walls. the pattern of extracellular proteins analysed by denaturing polyacrylamide gel electrophoresis ...200515928976
disease suppression on greenhouse tomatoes using plant waste compost.this study investigated the disease suppression abilities of a compost amendment that was added to the conventional growing medium, yellow cedar sawdust, used in most british columbia vegetable greenhouses. the compost amendment was produced in a controlled, in-vessel process primarily from greenhouse crop waste materials. the pathogen and cultivar under study were fusarium oxysporum f. sp. radicis-lycopersici (forl) on dombito (forl-susceptible) beefsteak greenhouse tomatoes. significant reduct ...200515913017
the strawberry gene cyf1 encodes a phytocystatin with antifungal est, encoding a strawberry phytocystatin (phycys) obtained from a developing fruit of fragariaxananassa cv. elsanta has been characterized. the corresponding gene (cyf1) had three introns interrupting its orf that codes for a protein (facpi-1) of 235 amino acid residues with a putative signal peptide of 29 residues and an estimated molecular mass for the mature protein of 23.1 kda. this protein contains, besides a c-terminal extension, several motifs conserved in all members of the phycys sup ...200515897228
vulgarinin, a broad-spectrum antifungal peptide from haricot beans (phaseolus vulgaris).from the seeds of haricot beans (phaseolus vulgaris), an antifungal peptide with a molecular mass around 7 kda was purified by using a simple protocol consisting of affinity chromatography on affi-gel blue gel and gel filtration on superdex 75. this peptide named vulgarinin manifested an antifungal activity toward fungal species such as fusarium oxysporum, mycosphaerella arachidicola, physalospora piricola and botrytis cinerea, and an antibacterial action on mycobacterium phlei, bacillus megater ...200515896669
secondary metabolites from endophytic streptomyces aureofaciens cmuac130 and their antifungal activity.streptomyces aureofaciens cmuac130 was isolated from the root tissue of zingiber officinale rosc. (zingiberaceae). it was an antagonist of colletotrichum musae and fusarium oxysporum, the causative agents of anthracnose of banana and wilt of wheat, respectively. evidence for the in vitro antibiosis of s. aureofaciens cmuac130 was demonstrated by the zone of fungal-growth inhibition. microscopic observations showed thickness and bulbous structures at the edges of the inhibited fungal hyphae. the ...200515870476
pleiotropic effect of phenolic compounds content increases in transgenic flax plant.the principal goal of this paper was to generate flax (linum usitatissimum l.) plants with increased antioxidant properties. to accomplish this a vector containing a multigene construct was prepared, and transgenic plants overexpressing essential flavonoid biosynthesis pathway enzymes were generated and analyzed. the simultaneous expression of genes encoding chalcone synthase (chs), chalcone isomerase (chi), and dihydroflavonol reductase (dfr) resulted in a significant increase of flax antioxida ...200515853420
effect of herbizid and touchdown herbicides on soil fungi and on production of some extracellular enzymes.glucophilic and cellulose-decomposing fungi were significantly reduced in soil samples treated with 0.019-0.152 mg a.i./kg soil of the herbicides herbizid and touchdown. the decrease was regularly correlated with the doses of the two herbicides and persisted till the end of the experiment (12 weeks). the isolated fungi were found to be able to produce hydrolytic extracellular enzymes in solid media but with variable capabilities. the ability to produce enzymes was adversily affected by the incor ...200515957238
drifter, a novel, low copy hat-like transposon in fusarium oxysporum is activated during starvation.the facultative pathogenic fungus fusarium oxysporum is known to harbour many different transposable and/or repetitive elements. we have identified drifter, a novel dna transposon of the hat family in f. oxysporum. it was found adjoining six1-h, a truncated homolog of the six1 avirulence gene in f. oxysporum f. sp. lycopersici. absence of a target site duplication as well as the 5' part of six1-h suggests that transposition of drifter into the ancestor of six1-h was followed by loss of a chromos ...200515869889
phaseococcin, an antifungal protein with antiproliferative and anti-hiv-1 reverse transcriptase activities from small scarlet runner beans.from the seeds of small scarlet runner beans (phaseolus coccineus 'minor'), an antifungal protein with an n-terminal sequence homologous to those of defensins was isolated. the antifungal protein bound to affi-gel blue gel and mono s but it did not bind to deae-cellulose. it was further purified by gel filtration on a superdex peptide column. it exhibited a molecular mass of 5422 da as determined by mass spectrometry. the protein, designated as phaseococcin, suppressed mycelial growth in a numbe ...200515864329
isolation and identification of antifungal and antialgal alkaloids from haplophyllum sieversii.bioassay-guided fractionation of the hexane/ethyl acetate/water (h/etoac/h2o) crude extract of the aerial parts of haplophyllum sieversii was performed because of preliminary screening data that indicated the presence of growth inhibitory components against colletotrichum fragariae, colletotrichum gloeosporioides, and colletotrichum acutatum. fractionation was directed using bioautographical methods resulting in the isolation of the bioactive alkaloids flindersine, anhydroevoxine, haplamine, and ...200516190626
production of an engineered killer peptide in nicotiana benthamiana by using a potato virus x expression system.the decapeptide killer peptide (kp) derived from the sequence of a single-chain, anti-idiotypic antibody acting as a functional internal image of a microbicidal, broad-spectrum yeast killer toxin (kt) was shown to exert a strong microbicidal activity against human pathogens. with the aim to exploit this peptide to confer resistance to plant pathogens, we assayed its antimicrobial activity against a broad spectrum of phytopathogenic bacteria and fungi. synthetic kp exhibited antimicrobial activit ...200516204558
quantitative assessment of phytopathogenic fungi in various substrates using a dna macroarray.detection, identification and quantification of plant pathogens are the cornerstones of preventive plant disease management. to detect multiple pathogens in a single assay, dna array technology currently is the most suitable technique. however, for sensitive detection, polymerase chain reaction (pcr) amplification before array hybridization is required. to evaluate whether dna array technology can be used to simultaneously detect and quantify multiple pathogens, a dna macroarray was designed and ...200516232285
enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria.our group studies tomato foot and root rot, a plant disease caused by the fungus forl (fusarium oxysporum f.sp. radicis-lycopersici ). several bacteria have been described to be able to control the disease, using different mechanisms. here we describe a method that enables us to select, after application of a crude rhizobacterial mixture on a sterile seedling, those strains that reach the root tip faster than our best tomato root colonizer tested so far, the pseudomonas fluorescens biocontrol st ...200516232295
antifungal effect of some spice hydrosols.the antifungal effects of rosemary, cumin, sater (savory), basil and pickling herb hydrosols were investigated against rhizoctonia solani, fusarium oxysporum f. sp tulipae, botrytis cinerea and alternaria citri. hydrosols of sater and pickling herb showed the most relevant fungicidal activity.200516243447
onychomycosis in children: a survey of 46 cases.this is a retrospective study of the agents, clinical aspects, sources of infection and therapy of onychomycosis in children. in the period 1989-2000, we observed 46 consecutive children, until 16 years of age with onychomycosis (29 boys, 17 girls, mean age 10.8 years). dermatophytes were isolated in 30 cases (trichophyton rubrum in 22 cases, trichophyton mentagrophytes in five, epidermophyton floccosum in two and trichophyton violaceum in one) and candida spp. in 16, associated with trichophyto ...200516262881
lunatusin, a trypsin-stable antimicrobial peptide from lima beans (phaseolus lunatus l.).an anti-fungal peptide designated as lunatusin, with a molecular mass around 7kda, was purified from the seeds of chinese lima bean (phaseolus lunatus l.). the peptide was isolated using a simple protocol consisting of affinity chromatography on affi-gel blue gel and gel filtration on superdex 75. lunatusin exerted an anti-fungal activity toward fungal species such as fusarium oxysporum, mycosphaerella arachidicola and botrytis cinerea, and an antibacterial action on, bacillus megaterium, bacill ...200516269344
variation in antibiosis ability, against potato pathogens, of bacterial communities recovered from the endo- and exoroots of potato crops produced under conventional versus minimum tillage systems.the culturable component of bacterial communities found in the endoroot and associated exoroot (root zone soil) was examined in potatoes (solanum tuberosum l.) grown under either conventional or minimum tillage systems. bacterial species--abundance relationships were determined and in vitro antibiosis ability investigated to discover whether tillage practice or bacteria source (endo- or exoroot) influenced bacterial community structure and functional versatility. antibiosis abilities against phy ...200516234863
fusarium oxysporum gas1 encodes a putative beta-1,3-glucanosyltransferase required for virulence on tomato plants.glycosylphosphatidylinositol-anchored (beta)-1,3-glucanosyltransferases play active roles in fungal cell wall biosynthesis and morphogenesis and have been implicated in virulence on mammals. the role of beta-1,3-glucanosyltransferases in pathogenesis to plants has not been explored so far. here, we report the cloning and mutational analysis of the gas1 gene encoding a putative beta-1,3-glucanosyltransferase from the vascular wilt fungus fusarium oxysporum. in contrast to candida albicans, expres ...200516353549
nep1 orthologs encoding necrosis and ethylene inducing proteins exist as a multigene family in phytophthora megakarya, causal agent of black pod disease on cacao.phvytophthora megakarya is a devastating oomycete pathogen that causes black pod disease in cacao. phytophthora species produce a protein that has a similar sequence to the necrosis and ethylene inducing protein (nep1) of fusarium oxysporum. multiple copies of nep1 orthologs (pmegnep) have been identified in p. megakarya and four other phytophthora species (p. citrophthora, p. capsici, p. palmivora, and p. sojae). genome database searches confirmed the existence of multiple copies of nep1 orthol ...200516353637
rapid differentiation of fusarium oxysporum isolates using pcr-sscp with the combination of ph-variable electrophoretic medium and low temperature.differentiation of fusarium oxysporum is significantly important for unraveling the pathogenetic mechanism of fusaria wilts. in this study, isolates of f. oxysporum were screened from the soils in the rhizosphere of watermelon plant by komada medium and differentiated by sscp approach with the combination of ph-variable electrophoretic medium (tris-mes-edta (tme), ph 6.1) and low temperature (9 degrees c). we found that tme was a good electrophoretic medium and its ph value was variable over the ...200516287185
an antifungal peptide from the coconut.a chromatographic procedure consisting of ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on cm-cellulose, and gel filtration by fast performance liquid chromatography on supedex 75 was utilized to isolate a 10 kda antifungal peptide from coconut flesh. the peptide was unadsorbed on deae-cellulose, but adsorbed on affi-gel blue gel and cm-cellulose. it displayed antifungal activity against fusarium oxysporum, mycosphaerella ...200516308082
the sen1 gene of arabidopsis is regulated by signals that link plant defence responses and senescence.plant defence and senescence share many similarities as evidenced by extensive co-regulation of many genes during these responses. to better understand the nature of signals that are common to plant defence and senescence, we studied the regulation of sen1 encoding a senescence-associated protein during plant defence responses in arabidopsis. pathogen inoculations and treatments with defence-related chemical signals, salicylic acid and methyl jasmonate induced changes in sen1 transcript levels. ...200516325410
gibberella xylarioides sensu lato from coffea canephora: a new mating population in the gibberella fujikuroi species complex.gibberella xylarioides heim & saccas (presumed anamorph, fusarium xylarioides steyaert) is the causal agent of coffee wilt disease, an economically important tracheomycosis in africa. in vitro crosses carried out with congolese, ugandan, and tanzanian single-ascospore/conidial isolates originating from diseased coffea canephora/excelsa demonstrated a heterothallic mating system, controlled by a single locus with two alleles, mat-1 and mat-2. compatible isolates produced fertile perithecia within ...200516332836
[ecological effects of multifunctional micro-flora agent in environment].in this paper, strains zjy-1 (brevibacillus brevis) and zjy-116 (bacillus subtilis) with evident inhibitory effect on fusarium oxysporum f. sp. cucumerinum and with promotion effect on plant growth were screened from cucumber rhizosphere, and the feasibility of exerting the ecological effects of multifunctional micro-flora agent was studied by treating cucumber seeds with the two strains singly or mixed with chlorpyrifos-degrading strain dsp3 (alcaligenes faecali). the results showed that the tw ...200516422513
fungi and nematodes occurring in different composts used in uruguayan agriculture.forty three thermophilic, thermotolerant and mesophilic fungal species and nematodes from five trophic groups were found in four compost types. aspergillus fumigatus and a. niger were the most frequent species. plant pathogenic fungi viz. fusarium oxysporum, f. acuminatum, f. nygamai and f. solani were found in some of the composts in low frequency. the most abundant species of nematode corresponded to bacterivores. tylenchus, a phytophagous nematode was scarcely found.200516459545
suppression of fusarium wilt by combining green compost and trichoderma hamatum.fusarium wilts, caused by the fungus fusarium oxysporum, are important diseases of horticultural and agricultural crops and lead to significant yield losses. the pathogen infects the roots and colonizes the vascular tissue, leading to wilting and finally death of the plant. the objective of this study was to investigate the efficacy of amendment of green compost and a trichoderma hamatum strain against fusarium wilt of radish. the substrate effects and the effect of a trichoderma strain were tes ...200516637174
biotic relation between fusarium oxysporum schlecht. and fungi isolated from the substrate of stewartia pseudocamellia (max.).the effect of the fungi community colonizing the substrate of stewartia plants on the growth of fusarium oxysporum schlecht. was investigated. the soil samples from 2 years old stewartia cuttings were taken for analysis in the second decade of october 2002 and 2003, when the symptoms of disease appeared on stewartia plant. fungi were isolated from the substrate using the sand method (mańka k. 1974). fusarium oxysporum were isolated from root system of stewartia pseudocamellia plant. the isolates ...200516637175
pathogenicity of two species of fusarium on some cultivars of bean in greenhouse.twenty isolates of fusarium oxysporum and f. solani were isolated from the infected roots of bean in different farms of east azarbaijan and tehran provinces and their pathogenicity determined. most isolates of the fungi were identified as f. oxysporun. they caused root rot, yellowing and wilting of bean in the field. in this test, the roots of 6 cultivars of bean seedlings soaked in suspension of the 7 isolates of the fungi (a1, gogan, a2, bilverdi, a3, savojbolagh-hashtgerd, a4, field of agr. c ...200516637191
[disseminated fusariosis by fusarium oxysporum in an adult patient with acute myeloid leukemia and severe febrile neutropenia].we report the first case of disseminated fusariosis in an adult patient in chile, with a hematologic neoplasia and chemotherapy that evolved with febrile prolonged neutropenia, refractory, treated with wide spectrum antibiotics and that developed a systemic infection with sinusal, cutaneous and pulmonary compromise by fusarium oxysporum. he was refractory to antifungal treatment with amphotericin b deoxycholate and caspofungin that were administered in a sequential schedule. the fatal outcome of ...200516341358
[heterologous expression and functional identification of a chitinase gene from wheat].chitinases are functional in plant defense against pathogens and also involved in plant development as well as human disease responses. in this study a chitinase gene, wch2, from wheat was expressed transiently in tobacco leaves and stably in transgenic arabidopsis thaliana plants to evaluate its heterologous expression and potential application. western blot analysis revealed that the expressed wheat chitinase was about 30 kd as was expected (fig.1) and had hydrolytic activity towards chitin po ...200516361785
chemical composition and antioxidant, antimicrobial, and antifungal activities of the essential oil of achillea ligustica all.the chemical composition of the essential oil from flowering tops of achillea ligustica all. was studied. samples were collected in different localities of sardinia (italy) and hydrodistilled both with clevenger-type and with simultaneous distillation-extraction apparatus. the yields ranged between 0.88 +/- 0.06 and 0.43 +/- 0.02% (vol/dry wt). the essential oils were analyzed by gc-ms, and a total of 96 components were detected. from a qualitative point of view, irrelevant differences between s ...200516366708
variability of non-mutualistic filamentous fungi associated with atta sexdens rubropilosa nests.a survey of the filamentous fungi other than the symbiotic one found in association with atta sexdens rubropilosa colonies was carried out. different fungal species (27 taxa) were isolated a few days after treating the workers with toxic baits (sulfluramid; mirex-s), from 40 laboratory and 20 field nests. syncephalastrum racemosum (54%) and escovopsis weberi (21%), trichoderma harzianum (38%) and fusarium oxysporum (23%) were the prevalent species in laboratory and field nests, respectively. acr ...200516475502
first report of a novel plant lysozyme with both antifungal and antibacterial activities.a novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4kda in sds-polyacrylamide gel electrophoresis was isolated from mung bean (phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on cm-sephadex, and high-performance liquid chromatography on poros hs-20. its n-terminal sequence was very different from that of hen egg white lysozyme. its pi was estimated to be above 9.7. the specific act ...200515649419
a genetic linkage map of lens sp. based on microsatellite and aflp markers and the localization of fusarium vascular wilt resistance.microsatellites have currently become the markers of choice for molecular mapping and marker-assisted selection for key traits such as disease resistance in many crop species. we report here on the mapping of microsatellites which had been identified from a genomic library of lentil (lens culinaris medik.). the majority of microsatellite-bearing clones contained imperfect di-nucleotide repeats. a total of 41 microsatellite and 45 amplified fragment length polymorphism (aflp) markers were mapped ...200515650814
evaluation of streptomyces sp. strain g10 for suppression of fusarium wilt and rhizosphere colonization in pot-grown banana plantlets.streptomyces sp. strain g10 exhibited strong antagonism towards fusarium oxysporum f.sp. cubense (foc) races 1, 2 and 4 in plate assays by producing extracellular antifungal metabolites. treating the planting hole and roots of 4-week-old tissue-culture-derived 'novaria' banana plantlets with strain g10 suspension (10(8) cfu/ml), significantly (p < 0.05) reduced wilt severity when the plantlets were inoculated with 10(4) spores/ml foc race 4. the final disease severity index for leaf symptom (lsi ...200515650871
isolation of alliumin, a novel protein with antimicrobial and antiproliferative activities from multiple-cloved garlic bulbs.a protein designated alliumin, with a molecular mass of 13 kda and an n-terminal sequence similar to a partial sequence of glucanase, and demonstrating antifungal activity against mycosphaerella arachidicola, but not against fusarium oxysporum, was isolated from multiple-cloved garlic (allium sativum) bulbs. the protein, designated as alliumin, was purified using ion exchange chromatography on deae-cellulose, cm-cellulose and mono s, affinity chromatography on affi-gel blue gel, and gel filtrati ...200515629528
new insights into the target site and mode of action of the antifungal protein of aspergillus giganteus.the antifungal protein (afp) secreted by aspergillus giganteus exerts growth inhibitory effects on various filamentous fungi. in order to obtain more information on the mode of action of afp, we used transmission electron microscopy in this study to compare the cellular ultrastructure of the afp-sensitive aspergillus niger and of the afp-resistant penicillium chrysogenum upon afp treatment. furthermore, afp was localized by immunogold staining in both fungi. severe membrane alterations in a. nig ...200515636747
molecular identification of fusarium species in onychomycoses.fusarium species are isolated from about 3% of onychomycoses in the swiss native population. on the basis of macroscopic characters and microscopic examination of the cultures, identification of fusarium often remains difficult or uncertain because of variations from one isolate to another and overlapping characteristics between species.200515604540
expression of the fusarium oxysporum lactonase gene in aspergillus oryzae: molecular properties of the recombinant enzyme and its application.the lactonase gene of fusarium oxysporum was expressed in aspergillus oryzae for optical resolution of dl-pantoyl lactone. when the chromosomal gene encoding the full-length form of the lactonase, which has its own nh2-terminal signal peptide, was introduced in the host cells, the resulting transformant produced an enzyme of 46,600 da, which corresponded to the wild-type enzyme. in contrast, a. oryzae transformed with the cdna coding the mature enzyme produced a protein of 41,300 da. deglycosyla ...200515503009
nitric oxide reductase (p450nor) from fusarium the present review we wanted to highlight the characteristic features of cytochtome p450 nadh-no reductase (p450nor) from fusarium oxysporum which belongs to the heme-thiolate protein family. this enzyme catalyzes the reduction of two no molecules to n2o. the discovery, isolation, identification and crystallography are described in detail. special emphasis was focused on the mechanism of no reduction and possible electronic configurations of the 444 nm intermediate were discussed. among heme- ...200515598501
nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-coa dehydrogenase.while several flavoproteins will oxidize nitroalkanes in addition to their physiological substrates, nitroalkane oxidase (nao) is the only one which does not require the anionic nitroalkane. this, in addition to the induction of nao by nitroethane seen in fusarium oxysporum, suggests that oxidation of a nitroaliphatic species is the physiological role of the enzyme. mechanistic studies of the reaction with nitroethane as substrate have established many of the details of the enzymatic reaction. t ...200515581574
fusarium oxysporum g-protein beta subunit fgb1 regulates hyphal growth, development, and virulence through multiple signalling pathways.the vascular wilt fungus fusarium oxysporum causes disease in a wide variety of crops. a signalling cascade controlled by the extracellular-regulated mitogen-activated protein kinase (mapk) fmk1 was previously found to be required for plant infection. to investigate the role of the heterotrimeric g-protein beta subunit fgb1 as a putative upstream component of the fmk1 signalling cascade, we generated f. oxysporum strains carrying either a deltafgb1 loss-of-function allele or an fgb1(w115g) allel ...200515588997
a pcr-denaturing gradient gel electrophoresis approach to assess fusarium diversity in north america, asparagus (asparagus officinalis) production suffers from a crown and root rot disease mainly caused by fusarium oxysporum f. sp. asparagi and f. proliferatum. many other fusarium species are also found in asparagus fields, whereas accurate detection and identification of these organisms, especially when processing numerous samples, is usually difficult and time consuming. in this study, a pcr-denaturing gradient gel electrophoresis (dgge) method was developed to assess fusariu ...200515590089
molecular markers linked to papaya ring spot virus resistance and fusarium race 2 resistance in melon, the fom-1 gene confers monogenic resistance against the soil-borne fungus fusarium oxysporum f. sp. melonis, races 0 and 2, while the closely linked prv gene specifies resistance against the papaya ring spot virus. markers linked to these resistance (r) genes were identified using two recombinant inbred line populations, derived from crosses between cucumis melo vedrantais and c. melo pi 161375, and between c. melo vedrantais and c. melo pi 414723, respectively. using bulked segregant ...200515551034
high-nitrogen compost as a medium for organic container-grown crops.compost was tested as a medium for organic container-grown crops. nitrogen (n) loss during composting of separated cow manure (scm) was minimized using high c/n (wheat straw, ws; grape marc, gm) or a slightly acidic (orange peels, op) additives. n conservation values in the resultant composts were 82%, 95% and 98% for gm-scm, op-scm and ws-scm, respectively. physical characteristics of the composts were compatible with use as growing media. the nutritional contribution of the composts was assess ...200515491822
biological control efficiency of fusarium wilt of tomato by nonpathogenic fusarium oxysporum fo-b2 in different environments.abstract efficiency of nonpathogenic fusarium oxysporum fo-b2 for the biological control of fusarium wilt of tomato, caused by f. oxysporum f. sp. lycopersici cu1, was examined in different environments: a growth chamber with sterile soil-less medium, a greenhouse with fumigated or nonfumigated soil, and nonfumigated field plots. inoculation of fo-b2 onto tomato roots significantly reduced the severity of disease, but the efficiency of disease suppression decreased as the experimental environmen ...200518943305
foliar spray of validamycin a or validoxylamine a controls tomato fusarium wilt.abstract tomato wilt, caused by the soilborne fungus fusarium oxysporum f. sp. lycopersici, is effectively controlled by a foliar spray of validamycin a (vma) or validoxylamine a (vaa) (>/=10 mug/ml); however, neither vma nor vaa is antifungal in vitro. in pot tests, the effect of a foliar application of vma or vaa at 100 mug/ml lasted for 64 days. plants sprayed with vma or vaa accumulated salicylic acid and had elevated expression of the systemic acquired resistance (sar) marker genes p4 (pr-1 ...200518943474
high genetic diversity among strains of fusarium oxysporum f. sp. vasinfectum from cotton in ivory coast.abstract seventeen isolates of fusarium oxysporum f. sp. vasinfectum from the ivory coast were characterized using vegetative compatibility group (vcg), restriction fragment length polymorphism of the ribosomal inter-genic spacer region (igs), and mating type (mat) idiomorph, and compared with a worldwide collection of the pathogen containing all available reference strains. some of the isolates were identical to known reference strains for all three traits, whereas others had previously unknown ...200518943549
development of crown and root rot disease of tomato under irrigation with saline water.abstract we studied the effect of water salinity on the incidence and severity of crown and root rot disease of tomato, as well as on the pathogen and on the plant's response to the pathogen. irrigation with saline water significantly increased disease severity in tomato transplants inoculated with fusarium oxysporum f. sp. radicis-lycopersici, and mineral fertilization further increased it. in one field experiment, disease incidence in plots irrigated with saline water (electrical conductivity ...200518943555
effect of mixed and single crops on disease suppressiveness of soils.abstract the effect of mixed cropping on disease suppressiveness of soils was tested for two cropping systems, brussels sprouts-barley and triticale-white clover. disease suppressiveness of field soils was evaluated in bioassays for the soilborne pathogens rhizoctonia solani, fusarium oxysporum f. sp. lini, and gaeumannomyces graminis var. tritici. for both cropping systems, mixed cropping did not enhance disease suppressiveness of the soils. in some cases, soil cropped to barley alone was signi ...200518943364
induction of beet-cyst nematode suppressiveness by the fungi dactylella oviparasitica and fusarium oxysporum in field microplots.abstract the ability of dactylella oviparasitica and fusarium oxysporum to suppress heterodera schachtii numbers was examined in field microplots. fungi were individually added to fumigated field soil that was seeded with sugar beet. four weeks later, soils were infested with h. schachtii second-stage juveniles (j2). at two harvests, 11 weeks and 19 weeks (1,469 and 2,547 degree days (base 8 degrees c), respectively) after nematode-infestation, h. schachtii cyst and egg numbers were assessed. at ...200618943750
molecular characterization of fusarium oxysporum and fusarium commune isolates from a conifer nursery.abstract fusarium species can cause severe root disease and damping-off in conifer nurseries. fusarium inoculum is commonly found in most container and bareroot nurseries on healthy and diseased seedlings, in nursery soils, and on conifer seeds. isolates of fusarium spp. can differ in virulence; however, virulence and colony morphology are not correlated. forty-one isolates of fusarium spp., morphologically indistinguishable from f. oxysporum, were collected from nursery samples (soils, healthy ...200618943501
suppression of the plant-parasitic nematode heterodera schachtii by the fungus dactylella oviparasitica.this study examined the role of the fungi dactylella oviparasitica and fusarium oxysporum in the beet-cyst nematode (heterodera schachtii) suppressiveness exhibited by a southern californian soil. in prior research, the abundance of d. oviparasitica rrna genes positively correlated with high levels of suppressiveness, whereas the abundance of f. oxysporum rrna genes positively correlated with minimal to moderate levels of suppressiveness. in this report, both fungi were added to fumigation-induc ...200618944211
a fusarium wilt resistance gene in gossypium barbadense and its effect on root-knot nematode-wilt disease complex.abstract fusarium wilt, caused by the soilborne pathogen fusarium oxysporum f. sp. vasinfectum race 1, is a vascular disease in cotton (gossypium spp.), and is a component of a disease complex with root-knot nematodes (meloidogyne incognita). genetic analysis of two interspecific crosses (g. barbadense pima s-7 x g. hirsutum acala nemx and pima s-7 x acala sj-2) showed that one major gene (designated fov1) with allele dosage effect conferred resistance to f. oxysporum f. sp. vasinfectum race 1 i ...200618943146
fusarium wilt of banana is caused by several pathogens referred to as fusarium oxysporum f. sp. cubense.abstract fusarium wilt of banana (also known as panama disease) is caused by fusarium oxysporum f. sp. cubense. where susceptible cultivars are grown, management is limited to the use of pathogen-free planting stock and clean soils. resistant genotypes exist for some applications, but resistance is still needed in other situations. progress has been made with this recalcitrant crop by traditional and nontraditional improvement programs. the disease was first reported in australia in 1876, but di ...200618943184
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