Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| novel protein from labramia bojeri a. dc. seeds homologue to kunitz-type trypsin inhibitor with lectin-like properties. | this study starts by isolating and characterizing the first protein from labramia bojeri seeds, which belong to the sapotaceae family. the purified lectin analyzed by sds-page with and without beta-mercaptoethanol shows two protein bands (m(r) = 19 and 20 kda), which cannot be resolved. protein bands have shown similar characteristics as molecular masses, determined by gel filtration and native gel; n-terminal sequences presented a difference in their isoelectric points. we have suggested that t ... | 2004 | 15675802 |
| comparative assessment of genotyping methods for study genetic diversity of fusarium oxysporum isolates. | in this study, we evaluated three pcr-based methods for the molecular typing of nonpathogenic fusarium oxysporum isolates: random amplified polymorphic dna (rapd), polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) and amplified fragment length polymorphism (aflp). the analyses were performed using 64 isolates of f. oxysporum collected from cotton-producing areas in egypt. a number of polymorphic rapd, pcr-rflp and aflp bands were scored in all isolates and the genetic ... | 2004 | 15702916 |
| effect of two systemic fungicides on cellulose decomposing fungi of tomato plants and on some enzymatic activities. | kocide 101 (77% cupric hydroxide) and ridomil plus (15% metalaxyl and 35% copper oxychloride) were used in the treatment of tomato plants. the two fungicides exerted a depressive effect on the total counts and on the individual cellulose decomposing fungal species associated with the roots and shoots of tomato. when these fungicides were incorporated in the liquid culture medium specified for growth and extracellular enzyme production by some selected fungal species, there was a significant redu ... | 2004 | 15704330 |
| relaxed primer specificity associated with reverse transcriptases encoded by the pfoxc retroplasmids of fusarium oxysporum. | the pfoxc mitochondrial retroplasmids are small, autonomously replicating linear dnas that have a telomere-like repeat of a 5-bp sequence at their termini. the plasmids are possible evolutionary precursors of the ribonucleoprotein complex telomerase, as they encode an active reverse transcriptase (rt) that is involved in plasmid replication. using an in vitro system to study reverse transcription, we show that the pfoxc rt is capable of copying in vitro-synthesized rnas by use of cdna primers or ... | 2004 | 15590832 |
| the influence of detergent, the active component of detergent and sodiumtripolyphosphate on the biochemical process of some fungi. | detergent "merix" (merima-krusevac) and its components, sodiumtripolyphosphate and ethoxyled oleyl-cetyl alcohol in defined concentrations have influence on the enzymatic activity, bioproduction of amino acids and proteins and total biomass of species aspergillus niger (a. niger) and fusarium oxysporum (f. oxysporum). detergent in concentration of 1% vol., in some cases, significantly stimulated production of the majority of amino acids. detergent and its components (ethoxyled oleyl-cetyl alcoho ... | 2004 | 15612193 |
| novel mode of resistance to fusarium infection by a mild dose pre-exposure of cadmium in wheat. | exposure of healthy wheat seeds (triticum aestivum var sonalika) to mild dose of cadmium (cd(2+)) given as 50 microm cdcl(2) for 48 h and then washed off cd(2+) offered resistance to the subsequent infection by fusarium oxysporum inoculum. seven days old seedlings having two primary leaves were aseptically inoculated with fungus, f. oxysporum (1 x 10(6)) spores. the seedlings pre-exposed to low level of cd(2+) survived the fusarium infection, while plantlets without cd(2+) stress wilted and then ... | 2004 | 15596097 |
| comparison of mesophilic and thermophilic feruloyl esterases: characterization of their substrate specificity for methyl phenylalkanoates. | the active sites of feruloyl esterases from mesophilic and thermophilic sources were probed using methyl esters of phenylalkanoic acids. only 13 out of 26 substrates tested were significant substrates for all the enzymes. lengthening or shortening the aliphatic side chain while maintaining the same aromatic substitutions completely abolished activity for both enzymes, which demonstrates the importance of the correct distance between the aromatic group and the ester bond. maintaining the phenylpr ... | 2004 | 15639097 |
| intracellular metabolite profiling of fusarium oxysporum converting glucose to ethanol. | the filamentous fungus fusarium oxysporum is known for its ability to produce ethanol by simultaneous saccharification and fermentation (ssf) of cellulose. however, the conversion rate is low and significant amounts of acetic acid are produced as a by-product. in this study, the growth characteristics of f. oxysporum were evaluated in a minimal medium using glucose as the sole carbon source in aerobic, anaerobic and oxygen-limited batch cultivations. under aerobic conditions the maximum specific ... | 2004 | 15639104 |
| aviglycine and propargylglycine inhibit conidial germination and mycelial growth of fusarium oxysporumf. sp. luffae. | two inhibitors, aviglycine and propargylglycine, were tested for their ability to suppress methionine synthesis thus inhibit conidial germination and mycelial growth of czapek-dox liquid medium grown fusarium oxysporum f. sp. luffaemum. the linear inhibition range for mycelial growth was about 7.6-762.9 microm. although aviglycine did not completely inhibit both conidial germination and mycelial growth, it showed significant inhibitory effect at 1.5 microm. the inhibition range for propargylglyc ... | 2004 | 15645176 |
| effects of the fermentation product of herbs by lactic acid bacteria against phytopathogenic filamentous fungi and on the growth of host plants. | the fermentation product of herbs by lactic acid bacteria (fhl) was assayed for antifungal activities against rosellinia necatrix, helicobasidium mompa, fusarium oxysporum, pythium graminicola and pyricularia oryzae. fhl completely inhibited the growth of r. necatrix, h. mompa, p. graminicola and p. oryzae, and reduced the growth of f. oxysporum by 35%. when the seeds of medicago sativa l. (alfalfa), asparagus officinalis l. (asparagus), brassica campestris l. (komatsuna), oryza sativa l. (rice) ... | 2004 | 16233688 |
| evaluation of nostoc strain atcc 53789 as a potential source of natural pesticides. | the cyanobacterium nostoc strain atcc 53789, a known cryptophycin producer, was tested for its potential as a source of natural pesticides. the antibacterial, antifungal, insecticidal, nematocidal, and cytotoxic activities of methanolic extracts of the cyanobacterium were evaluated. among the target organisms, nine fungi (armillaria sp., fusarium oxysporum f. sp. melonis, penicillium expansum, phytophthora cambivora, p. cinnamomi, rhizoctonia solani, rosellinia, sp., sclerotinia sclerotiorum, an ... | 2004 | 15184126 |
| disseminated fusarium oxysporum infection in hemophagocytic lymphohistiocytosis. | the portal of entry of disseminated fusarium spp. infections is still not clearly defined. we report on a disseminated fusarium oxysporum infection occurring during a long period of severe neutropenia in a child with hemophagocytic lymphohistiocytosis. a nasogastric feeding tube was the possible source of entry of the fungus. | 2004 | 15597228 |
| indigenous bacteria with antagonistic and plant-growth-promoting activities improve slow-filtration efficiency in soilless cultivation. | in tomato soilless culture, slow filtration allows one to control the development of diseases caused by pathogenic microorganisms. during the disinfecting process, microbial elimination is ensured by mechanical and biological factors. in this study, system efficacy was enhanced further to a biological activation of filter by inoculating the pozzolana grains contained in the filtering unit with 5 selected bacteria. three strains identified as pseudomonas putida and 2 as bacillus cereus came from ... | 2004 | 15381975 |
| crystallization and preliminary analysis of active nitroalkane oxidase in three crystal forms. | nitroalkane oxidase (nao), a flavoprotein cloned and purified from fusarium oxysporum, catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones, with the production of h2o2 and nitrite. in this paper, the crystallization and preliminary x-ray data analysis of three crystal forms of active nitroalkane oxidase are described. the first crystal form belongs to a trigonal space group (either p3(1)21 or p3(2)21, with unit-cell parameters a = b = 103.8, c = 487.0 a) and ... | 2004 | 15272176 |
| biological synthesis of strontium carbonate crystals using the fungus fusarium oxysporum. | the total biological synthesis of srco3 crystals of needlelike morphology arranged into higher order quasi-linear superstructures by challenging microorganisms such as fungi with aqueous sr2+ ions is described. we term this procedure "total biological synthesis" since the source of carbonate ions that react with aqueous sr2+ ions is the fungus itself. we believe that secretion of proteins during growth of the fungus fusarium oxysporum is responsible for modulating the morphology of strontianite ... | 2004 | 15274591 |
| a small, cysteine-rich protein secreted by fusarium oxysporum during colonization of xylem vessels is required for i-3-mediated resistance in tomato. | a 12 kda cysteine-rich protein is secreted by fusarium oxysporum f. sp. lycopersici during colonization of tomato xylem vessels. peptide sequences obtained with mass spectrometry allowed identification of the coding sequence. the gene encodes a 32 kda protein, designated six1 for secreted in xylem 1. the central part of six1 corresponds to the 12 kda protein found in xylem sap of infected plants. a mutant that had gained virulence on a tomato line with the i-3 resistance gene was found to have l ... | 2004 | 15387816 |
| a rapid molecular method for differentiating two special forms (lycopersici and radicis-lycopersici) of fusarium oxysporum. | two pathogenic special forms (f. sp.) of the fusarium oxysporum species complex f. sp. lycopersici (fol) and f. sp. radicis-lycopersici (forl) are morphologically indistinguishable. although they are pathogenic to the same host genus lycopersicon (tomato), and infect the same tomato cultivar, they form distinct diseases; fol causes wilt and forl causes crown rot and root rot. these two special forms apparently exist as genetically isolated populations, based on vegetative compatibility and molec ... | 2004 | 15446712 |
| anti-fungal sesquiterpenoid from the root exudate of solanum abutiloides. | the solanum abutiloides plant is highly resistant to soil-borne pathogens such as fusarium oxysporum f. sp. melongenae, verticillium dahliae, and ralstonia solanacearum. this species is utilized as a mating source of resistant cultivars and is also used as a rootstock. the root exudate of solanum abutiloides was extracted from a soil system composed of charcoal and vermiculite. anti-fungal activity was found in the extract, and an active ingredient was isolated. the chemical structure of the act ... | 2004 | 15618642 |
| novel oligosaccharides isolated from fusarium oxysporum l. rapidly induce pal activity in rubus cells. | activation of the phenolic pathway is known to be part of a defense response against cell wall-derived elicitors from pathogens. many examples of a defense response by increasing the synthesis of phenolic compound against the elicitor were demonstrated in the past, but the elicitor structure has so far been poorly characterized. our results indicate that a disaccharide fraction containing the following structure: alpha-d-mannopyranosyl (1-->2)alpha/beta-d-glucopyranosyl and alpha-d-mannopyranosy ... | 2004 | 15448725 |
| the fusarium oxysporum f. sp. ciceris/cicer arietinum pathosystem: a case study of the evolution of plant-pathogenic fungi into races and pathotypes. | the use of resistant cultivars is one of the most practical and cost-efficient strategies for managing plant diseases. however, the efficiency of resistant cultivars in disease management is limited by pathogenic variability in pathogen populations. knowledge of the evolutionary history and potential of the pathogen population may help to optimize the management of disease-resistance genes, irrespective of the breeding strategy used for their development. in this review, we examine the diversity ... | 2004 | 15248157 |
| [variation of microflora and enzyme activity in continuous cropping cucumber soil in solar greenhouse]. | variation of microflora and enzyme activity in solar greenhouse soil continuous cropping for 1, 3, 5, 7, 9 years was studied, in addition to the relationship between soil properities and microflora and enzyme activity. the results showed that number of bacteria, actinomyce as well as total microflora showed a trend with a saddle-shaped curve, while the number of fungi appeared an liner increase. continuous cropping soil microflora shifted from bacteria type to fungi type significantly, of which ... | 2004 | 15362624 |
| tomatidine and lycotetraose, hydrolysis products of alpha-tomatine by fusarium oxysporum tomatinase, suppress induced defense responses in tomato cells. | many fungal pathogens of tomato produce extracellular enzymes, collectively known as tomatinases, that detoxify the preformed antifungal steroidal glycoalkaloid alpha-tomatine. tomatinase from the vascular wilt pathogen of tomato fusarium oxysporum f. sp. lycopersici cleaves alpha-tomatine into the aglycon tomatidine (td) and the tetrasaccharide lycotetraose (lt). although modes of action of alpha-tomatine have been extensively studied, those of td and lt are poorly understood. here, we show tha ... | 2004 | 15280013 |
| coccinin, an antifungal peptide with antiproliferative and hiv-1 reverse transcriptase inhibitory activities from large scarlet runner beans. | an antifungal peptide, designated coccinin, with a molecular mass of 7kda and an n-terminal sequence resembling those of defensins, was purified from the seeds of large scarlet runner beans (phaseolus coccineus cv. 'major'). the peptide isolated was unadsorbed on deae-cellulose, and adsorbed on affi-gel blue gel and mono s. the peptide excerted antifungal activity on a number of fungal species including botrytis cinerea, coprinus comatus, fusarium oxysporum, mycosphaerella arachidicola, physalos ... | 2004 | 15572193 |
| antifungal peptides, a heat shock protein-like peptide, and a serine-threonine kinase-like protein from ceylon spinach seeds. | two antifungal peptides (designated alpha- and beta-basrubrins) with molecular masses of 4-5 kda and distinct n-terminal sequences, and a peptide and a protein with n-terminal sequences resembling heat shock protein (hsp) and serine-threonine kinase, respectively, were isolated from seeds of the ceylon spinach basella rubra. the purification procedure entailed saline extraction, (nh4)2so4 precipitation, ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ... | 2004 | 15245882 |
| gene expression profile changes in cotton root and hypocotyl tissues in response to infection with fusarium oxysporum f. sp. vasinfectum. | microarray analysis of large-scale temporal and tissue-specific plant gene expression changes occurring during a susceptible plant-pathogen interaction revealed different gene expression profile changes in cotton root and hypocotyl tissues. in hypocotyl tissues infected with fusarium oxysporum f. sp. vasinfectum, increased expression of defense-related genes was observed, whereas few changes in the expression levels of defense-related genes were found in infected root tissues. in infected roots, ... | 2004 | 15195948 |
| [germination of phytopathogenic fungi conidia]. | the autoregulation of conidium germination in phytopathogenic micromycetes of the genera fusarium, botrytis, and bipolaris was studied. it was shown that trichoderma longibrachiatum was less competitive than fusarium oxysporum after their simultaneous inoculation but inhibited the phytopathogen growth in the case of earlier introduction. in the latter case, no autoinhibition of the germination of f. oxysporum conidia occurred; moreover, cooperative effect was observed, i.e., the number of germin ... | 2004 | 15521169 |
| response of embryo axes of germinating seeds of yellow lupine to fusarium oxysporum. | defence responses of embryo axes of lupinus luteus l. cv. polo were studied 48-96 h after inoculation with fusarium oxysporum schlecht f.sp. lupini. the infection restricted the growth of embryo axes, the lengths of infected embryo axes 72 and 96 h after inoculation were 11 and 12 mm less in the controls, respectively, while their masses c. 0.03 g less than in the controls. the concentration of h2o2 in embryo axes of inoculated germinating seeds was higher than in the control. this was probably ... | 2004 | 15246062 |
| colonization and persistence of a plant growth-promoting bacterium pseudomonas fluorescens strain cs85, on roots of cotton seedlings. | pseudomonas fluorescens cs85, which was previously isolated from the rhizosphere of cotton seedlings, acts as both a plant growth-promoting bacterium and a biocontrol agent against cotton pathogens, including rhizoctonia solani, colletotrichum gossypii, fusarium oxysporum f sp. vasinfectum, and verticillium dahliae. strain cs85 was labeled separately with luxab and gusa. the labeled strains were stably maintained and had high levels of expression of the marker genes, luxab and gusa, after succes ... | 2004 | 15381971 |
| onychomycosis caused by fusarium solani and fusarium oxysporum in são paulo, brazil. | fusarium species are common soil saprophytes and plant pathogens that have been frequently reported as etiologic agents of opportunistic infections in humans. we report eight cases of onychomycosis caused by fusarium solani (4) and fusarium oxysporum (4) in são paulo, brazil. these species were isolated from toenails in all cases. the infections were initially considered to be caused by dermatophytes. the clinical appearance of the affected toenails was leukonychia or distal subungual hyperkerat ... | 2004 | 15180157 |
| antifungal compounds from the root and root exudate of zea mays. | a maize plant (zea mays) planted in a test tube was found to inhibit the growth of the soil-borne plant pathogen, fusarium oxysporum f. sp. melongenae. the antifungal compounds, 6-methoxybenzoxazolinone and 6,7-dimethoxybenzoxazolinone, were isolated from an ethanol extract of zea mays roots, and (6r)-7,8-dihydro-3-oxo-alpha-ionone and (6r,9r)-7,8-dihydro-3-oxo-alpha-ionol were isolated from the root exudate. | 2004 | 15215605 |
| frequency and diversity of fungi colonizing tissues of upland cotton. | a study of the mycoflora of upland cotton in alabama was conducted throughout the 2000 and 2001 growing seasons. plants were sampled at seedling, first bloom, full bloom, and maturity stages of development. thirty-seven genera representing 58 species of fungi were isolated, including 9 species of fusarium. fusarium oxysporum, f. solani, and f. equiseti were the most common members of this genus occurring at all four sampling stages in both years. eight species accounted for 67% of the total fung ... | 2004 | 15180159 |
| 1-phenyl-3-toluyl-4-[ortho-1'-(n-ethyl-2'-methylpropylamine)]phenylpyrazole, synthesis and evaluation of the in vitro antifungal activity against botrytis cinerea and fusarium oxysporum. | a novel antifungal pyrazole derivative was synthesized. designated 1-phenyl-3-toluyl-4-[ortho-1'-(n-ethyl-2'-methylpropylamine)]phenylpyrazole, the compound exerted an antifungal effect toward botrytis cinerea and fusarium oxysporum. in fact, our results clearly show that mycelial growth and conidial germination of both fungi were blocked by the compound. indeed, a 96-well microbioassay procedure was used for fast and easy evaluation of minimal inhibitory concentration (mic). the mic values for ... | 2004 | 15337431 |
| light- and singlet oxygen-mediated antifungal activity of phenylphenalenone phytoalexins. | the light-induced singlet oxygen production and antifungal activity of phenylphenalenone phytoalexins isolated from infected banana plants (musa acuminata) are reported. upon absorption of light energy all studied phenylphenalenones sensitise the production of singlet oxygen in polar and non-polar media. antifungal activity of these compounds towards fusarium oxysporum is enhanced in the presence of light. these results, together with the correlation of ic50 values under illumination with the qu ... | 2004 | 15239009 |
| ethylene response factor 1 mediates arabidopsis resistance to the soilborne fungus fusarium oxysporum. | ethylene response factor 1 (erf1) is a transcriptional factor from arabidopsis thaliana that regulates plant resistance to the necrotrophic fungi botrytis cinerea and plectosphaerella cucumerina and whose overexpression enhances resistance to these fungi. here, we show that erf1 also mediates arabidopsis resistance to the soilborne fungi fusarium oxysporum sp. conglutinans and f. oxysporum f. sp. lycopersici, because its constitutive expression in arabidopsis confers enhanced resistance to these ... | 2004 | 15242170 |
| in vitro fungitoxic activity of larrea divaricata cav. extracts. | to evaluate the fungitoxic activity of larrea divaricata cav. extract and one of its components against yeasts and fungi. this activity was compared with the action of ketoconazole, a known synthetic antimycotic. | 2004 | 15189281 |
| actinchinin, a novel antifungal protein from the gold kiwi fruit. | an antifungal protein designated actinchinin, with an n-terminal sequence different from that of the thaumatin-like antifungal protein from green kiwi fruit, was isolated from the gold kiwi fruit. the antifungal protein, unlike its counterpart from green kiwi fruit, did not exert antifungal activity against botrytis cinerea, but was active against fusarium oxysporum which was unresponsive to thaumatin-like protein from green kiwi fruit. actinchinin was isolated using a protocol that comprised io ... | 2004 | 15245867 |
| effect of static magnetic field on growth and sporulation of some plant pathogenic fungi. | growth and sporulation of phytopathogenic microscopic fungi were studied under a static magnetic field. the applied flux densities were 0.1, 0.5, and 1 mt. the magnetic field decreased the growth of colonies by 10% using this flux density region. at 0.1 mt flux density, the deviations are significant, p =.001, while in other cases the deviations generally are not significant. at the same time, the number of the developed conidia of alternaria alternata and curvularia inaequalis increased by 68-1 ... | 2004 | 15114642 |
| an analysis of the phylogenetic distribution of the pea pathogenicity genes of nectria haematococca mpvi supports the hypothesis of their origin by horizontal transfer and uncovers a potentially new pathogen of garden pea: neocosmospora boniensis. | the filamentous fungus nectria haematococca mating population vi (mpvi) contains a cluster of genes required to cause disease on pea. this cluster of pea pathogenicity genes (the pep cluster) is located on a supernumerary chromosome that is dispensable for normal growth in culture. the genes in the pep cluster have a different g+c content and codon usage compared with the genes located on the other chromosomes and a non-homogeneous distribution within the species. these features suggest that the ... | 2004 | 15118835 |
| antifungal constituents of clytostoma ramentaceum and mansoa hirsuta. | ethanol extracts of clytostoma ramentaceum bur. & k. schum and mansoa hirsuta dc. (bignoniaceae) inhibited the growth of standardized cultures of aspergillus niger and fusarium oxysporum, at concentrations of 400 microg and 500 microg, in bioautographic assays. the activity-guided fractionation of c. ramentaceum extract afforded ursolic acid and 2-(3',4'-dihydroxyphenyl) ethanol, both active against the test fungi (100 microg). these compounds are reported for the first time in c. ramentaceum an ... | 2004 | 15287070 |
| immobilization of biogenic gold nanoparticles in thermally evaporated fatty acid and amine thin films. | we have recently demonstrated the biological synthesis of gold nanoparticles by the reduction of aqueous chloroaurate ions by the fungus fusarium oxysporum and with extract of geranium (pelargonium graveolens) leaf. in this paper, we demonstrate the immobilization of biogenic gold nanoparticles in lipid thin films deposited by thermal evaporation. the charge on the gold nanoparticles synthesized by both the fungus and the geranium plant extract is used to facilitate their immobilization in both ... | 2004 | 15120279 |
| siderophore production by a marine pseudomonas aeruginosa and its antagonistic action against phytopathogenic fungi. | a marine isolate of fluorescent pseudomonas sp. having the ability to produce the pyoverdine type of siderophores under low iron stress (up to 10 microm iron in the succinate medium) was identified as pseudomonas aeruginosa by using biolog breathprint and siderotyping. pyoverdine production was optimum at 0.2% (w/v) succinate, ph 6.0, in an iron-deficient medium. studies carried out in vitro revealed that purified siderophores and pseudomonas culture have good antifungal activity against the pla ... | 2004 | 15304753 |
| a ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom pleurotus sajor-caju. | a 12 kda ribonuclease preferential for poly u and with much lower activity toward poly a, poly g and poly c was isolated from fresh fruiting bodies of the mushroom pleurotus sajor-caju. a purification procedure involving ion exchange chromatography on cm-cellulose, affinity chromatography on red-sepharose and heparin-sepharose, and fast protein liquid chromatography-gel filtration on superdex 75 was used. the ribonuclease was adsorbed on all of the first three types of chromatographic media. it ... | 2004 | 15003351 |
| fungus-elicited metabolites from plants as an enriched source for new leishmanicidal agents: antifungal phenyl-phenalenone phytoalexins from the banana plant (musa acuminata) target mitochondria of leishmania donovani promastigotes. | two antifungal phenyl-phenalenone phytoalexins isolated from the banana plant (musa acuminata) elicited with the fungus fusarium oxysporum, together with a methoxy derivative of one of them and two epoxide precursors of their chemical synthesis, were tested for leishmanicidal activity on leishmania donovani promastigotes and l. infantum amastigotes. drugs inhibited proliferation of both forms of the parasite with a 50% lethal concentration range between 10.3 and 68.7 micro g/ml. their lethal mec ... | 2004 | 15105102 |
| enzymatic liberation of lycotetraose from the solanum glycoalkaloid alpha-tomatine. | the branched tetrasaccharide, o-beta-d-glucopyranosyl-(1-->2)-o-[beta-d-xylopyranosyl-(1-->3)]-o-beta-d-glucopyranosyl-(1-->4)-d-galactose (lycotetraose) is a key constituent of many biologically interesting natural products. described herein is a convenient enzymatic preparation of lycotetraose from the readily available solanum glycoalkaloid alpha-tomatine. the preparation makes use of the recombinant endo-glycosidase, tomatinase, from the plant pathogen fusarium oxysporum f. sp. lycopersici. | 2004 | 15337463 |
| a non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean. | a non-specific lipid transfer peptide (nsltp) with antimicrobial activity was isolated from the mung bean (phaseolus mungo) seeds. the procedure entailed aqueous extraction, ion exchange chromatography on cm-sephadex and high performance liquid chromatography (hplc) on poros-hs-20. the peptide exhibited a molecular mass of 9.03 kda in mass spectrometry. it exerted antifungal action toward fusarium solani, fusarium oxysporum, pythium aphanidermatum and sclerotium rolfsii, and antibacterial action ... | 2004 | 15350690 |
| alveolarin, a novel antifungal polypeptide from the wild mushroom polyporus alveolaris. | an antifungal polypeptide, with a molecular mass of 28 kda as judged by gel filtration and appearing as a single band with a molecular mass of 14 kda in sodium dodecyl suflate-polyacrylamide gel electrophoresis, was isolated from fresh fruiting bodies of the mushroom polyporus alveolaris. the antifungal polypeptide, designated as alveolarin, demonstrated an inhibitory action on mycelial growth in botrytis cinerea, fusarium oxysporum, mycosphaerella arachidicola and physalospora piricola. alveola ... | 2004 | 15165727 |
| development of selective media for the isolation and enumeration of alternaria species from soil and plant debris. | a new semi-selective medium, acidified weak potato-dextrose agar (awpda) with mertect (active ingredient: thiabendazole), was developed for the isolation and enumeration of alternaria species from samples of soil and plant debris. the medium was selected based on growth inhibition tests against alternaria and several other commonly encountered saprobic fungi utilizing three antifungal agents, botran (active ingredient: dichloran), bayleton (active ingredient: triadimefon), and mertect, and two b ... | 2004 | 15381969 |
| eryngin, a novel antifungal peptide from fruiting bodies of the edible mushroom pleurotus eryngii. | an antifungal peptide with a molecular mass of 10k da was isolated from fruiting bodies of the mushroom pleurotus eryngii. the peptide, designated as eryngin, inhibited mycelial growth in fusarium oxysporum and mycosphaerella arachidicola. it was unadsorbed on deae-cellulose and adsorbed on affi-gel blue gel and s-sepharose. its n-terminal sequence demonstrated some similarity to the antifungal protein from the mushroom lyophyllum shimeiji and little resemblance to thaumatin and thaumatin-like p ... | 2004 | 15003349 |
| the role of the jasmonate response in plant susceptibility to diverse pathogens with a range of lifestyles. | plants defend themselves against attack from insects and pathogens with various resistance strategies. the jasmonate and salicylate signaling pathways are two induced responses that protect plants against these attackers. knowledge of the range of organisms that are affected by each response is important for understanding how plants coordinate their defenses against multiple attackers and the generality of effect of different resistance mechanisms. the jasmonate response is known to protect plan ... | 2004 | 15133157 |
| fusion proteins comprising a fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. | in planta expression of recombinant antibodies recognizing pathogen-specific antigens has been proposed as a strategy for crop protection. we report the expression of fusion proteins comprising a fusarium-specific recombinant antibody linked to one of three antifungal peptides (afps) as a method for protecting plants against fungal diseases. a chicken-derived single-chain antibody specific to antigens displayed on the fusarium cell surface was isolated from a pooled immunocompetent phage display ... | 2004 | 15146196 |
| antimicrobial effects of quercus ilex l. extract. | the antimicrobial activities of the methanol extract of quercus ilex l. (pirnal oak) leaves were tested in vitro against a wide range of human and plant-associated microorganisms. a total of 132 microbial organisms belonging to 55 bacteria and five fungi and yeast species were studied using a disc-diffusion method and microdilution assays. the results were evaluated as inhibition zones around the disc impregnated with q. ilex extract at a concentration of 300 micro l/ml. the results showed that ... | 2004 | 15103667 |
| combination therapy of disseminated fusarium oxysporum infection with terbinafine and amphotericin b. | a case of disseminated infection with fusarium oxysporum following chemotherapy of acute myelogenous leukemia is reported. antifungal treatment was successful with a 13-day course of oral terbinafine 250 mg t.i.d. in combination with amphotericin b deoxycholate 1.0-1.5 mg/kg qd and subsequently intravenous liposomal amphotericin b 5 mg/kg qd. preceding monotherapy with amphotericin b deoxycholate 1.0-1.5 mg/kg qd had not stopped the progression of infection. the combination therapy described her ... | 2004 | 14648020 |
| isolation of allicepin, a novel antifungal peptide from onion (allium cepa) bulbs. | from the bulbs of the onion allium cepa, a novel antifungal peptide distinct from the antimicrobial peptide previously reported from onion seeds was isolated. the antifungal peptide, designated allicepin, was purified with a procedure that involved aqueous extraction, ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel and fplc-gel filtration on superdex 75. allicepin was unadsorbed on deae-cellulose and adsorbed on affi-gel blue gel. the molecular weight ... | 2004 | 15113089 |
| ren1 is required for development of microconidia and macroconidia, but not of chlamydospores, in the plant pathogenic fungus fusarium oxysporum. | the filamentous fungus fusarium oxysporum is a soil-borne facultative parasite that causes economically important losses in a wide variety of crops. f. oxysporum exhibits filamentous growth on agar media and undergoes asexual development producing three kinds of spores: microconidia, macroconidia, and chlamydospores. ellipsoidal microconidia and falcate macroconidia are formed from phialides by basipetal division; globose chlamydospores with thick walls are formed acrogenously from hyphae or by ... | 2004 | 15020411 |
| construction of a hindiii bacterial artificial chromosome library and its use in identification of clones associated with disease resistance in chickpea. | a chickpea ( cicer arietinum l.) bacterial artificial chromosome (bac) library from germplasm line, flip 84-92c, was constructed to facilitate positional cloning of disease resistance genes and physical mapping of the genome. the bac library has 23,780 colonies and was calculated to comprise approximately 3.8 haploid-genome equivalents. studies on 120 randomly chosen clones revealed an average insert size of 100 kb and no empty clones. colony hybridization using the rubp carboxylase large subuni ... | 2004 | 14564396 |
| specific detection of the toxigenic species fusarium proliferatum and f. oxysporum from asparagus plants using primers based on calmodulin gene sequences. | fusarium proliferatum and fusarium oxysporum are the causal agents of a destructive disease of asparagus called fusarium crown and root rot. f. proliferatum from asparagus produces fumonisin b1 and b2, which have been detected as natural contaminants in infected asparagus plants. polymerase chain reaction (pcr) assays were developed for the rapid identification of f. proliferatum and f. oxysporum in asparagus plants. the primer pairs are based on calmodulin gene sequences. the pcr products from ... | 2004 | 14757245 |
| synthesis, absolute stereochemistry and molecular design of the new antifungal and antibacterial antibiotic produced by streptomyces sp.201. | the absolute stereochemistry of the new antifungal and antibacterial antibiotic produced by streptomyces sp.201 has been established by achieving the total synthesis of the product. a series of analogues have also been synthesized by changing the side chain and their bioactivity assessed against different microbial strains. among them, 1e (r = c8h17) was found to be the most potent with mic of 8 microg/ml against mycobacterium tuberculosis, 12 microg/ml against escherichia coli and 16 microg/ml ... | 2004 | 15177476 |
| nitrous oxide-forming codenitrification catalyzed by cytochrome p450nor. | intact cells of the denitrifying fungus fusarium oxysporum were previously shown to catalyze codenitrification to form a hybrid nitrous oxide (n2o) species from nitrite and other nitrogen compounds such as azide and ammonia. here we show that cytochrome p450nor can catalyze the codenitrification reaction to form n2o from nitric oxide (no) but not nitrite, and azide or ammonia. the results show that the direct substrate of the codenitrification by intact cells should not be nitrite but no, which ... | 2004 | 14981321 |
| fusarium species and fusarium wilt pathogens associated with native gossypium populations in australia. | fusarium isolates were extracted from stems and rhizosphere soils of 79 populations of four gossypium species native to two regions of inland australia in 2001. six fusarium species were isolated from 31 (3%) of the 919 stem samples. f. semitectum was predominant, occurring mostly in g. bickii populations and accounting for 81% of the isolates recovered. twenty fusarium species were isolated from 68 (87%) of the 78 composite soil samples, with f. solani (71%), f. compactum (8%), f. oxysporum (5% ... | 2004 | 15035503 |
| extracellular matrix protein gene, emp1, is required for appressorium formation and pathogenicity of the rice blast fungus, magnaporthe grisea. | magnaporthe grisea, the causal fungus of rice blast, forms a specialized infection structure called an appressorium that is crucial for host plant penetration. a cdna clone of m. grisea, showing strong sequence homology to fem1 of fusarium oxysporum and encoding an extracellular matrix protein, was isolated during an expressed sequence tag (est) analysis of an appressorium cdna library and named extracellular matrix protein 1 (emp1). sequence analysis of the corresponding genomic clone revealed ... | 2004 | 15055545 |
| a new class (penaeidin class 4) of antimicrobial peptides from the atlantic white shrimp (litopenaeus setiferus) exhibits target specificity and an independent proline-rich-domain function. | a highly pure, chemically defined representative of a new class of antimicrobial peptide from the atlantic white shrimp (litopenaeus setiferus), penaeidin class 4 [pen4-1 (penaeidin class 4 isoform 1)], was produced synthetically. chemical synthesis was achieved by native ligation from two separate domains yielding a bioactive peptide that reflected the characteristics of native penaeidin. synthetic pen4-1 proved to be an effective antimicrobial peptide, particularly against the broad-spectrum p ... | 2004 | 15084144 |
| potential role of pathogen signaling in multitrophic plant-microbe interactions involved in disease protection. | multitrophic interactions mediate the ability of fungal pathogens to cause plant disease and the ability of bacterial antagonists to suppress disease. antibiotic production by antagonists, which contributes to disease suppression, is known to be modulated by abiotic and host plant environmental conditions. here, we demonstrate that a pathogen metabolite functions as a negative signal for bacterial antibiotic biosynthesis, which can determine the relative importance of biological control mechanis ... | 2004 | 15006813 |
| fine mapping of the tomato i-3 gene for fusarium wilt resistance and elimination of a co-segregating resistance gene analogue as a candidate for i-3. | the i-3 gene from the wild tomato species lycopersicon pennellii confers resistance to race 3 of the devastating vascular wilt pathogen fusarium oxysporum f. sp. lycopersici. as an initial step in a positional cloning strategy for the isolation of i-3, we converted restriction fragment length polymorphism and conserved orthologue set markers, known genes and a resistance gene analogue (rga) mapping to the i-3 region into pcr-based sequence characterised amplified region (scar) and cleaved amplif ... | 2004 | 15045176 |
| improved technique for rapid and non-destructive in vitro differentiation between resistant and susceptible banana clones of fusarium oxysporum f. sp. cubense. | culture filtrates of fusarium oxysporum f. sp. cubense were applied to field-grown banana leaves of susceptible and resistant clones. the difference in leaf lesions, measured after 48 h, varied from 13 to 51 mm2 depending on the composition of the growth medium. | 2004 | 15049365 |
| cloning and functional expression of a mungbean defensin vrd1 in pichia pastoris. | it was shown previously that a bacterially expressed mungbean defensin vrcrp exhibited both antifungal and insecticidal activities. to isolate this protein in a large quantity for its characterization, the defensin cdna was expressed in pichia pastoris and the recombinant defensin (rvrd1) was purified. the recombinant vrd1 was shown to inhibit the growth of fungi such as fusarium oxysporum, pyricularia oryza, rhizoctonia solani, and trichophyton rubrum and development of bruchid larva. the prote ... | 2004 | 15080630 |
| physical and biochemical interactions of soil fungi with asbestos fibers. | iron, a structural component of most asbestos, is thought to play a crucial role in asbestos toxicity. surface iron favors fiber uptake by mammalian cells and promotes the generation of free radicals with consequent dna and lipid damage. in some cases, chemical removal of iron from asbestos fibers has reduced several cellular responses to asbestos. since iron represents an essential element for soil microorganisms, many of them have developed mechanisms to scavenge this element from poorly solub ... | 2004 | 15095889 |
| fungal ammonia fermentation, a novel metabolic mechanism that couples the dissimilatory and assimilatory pathways of both nitrate and ethanol. role of acetyl coa synthetase in anaerobic atp synthesis. | fungal ammonia fermentation is a novel dissimilatory metabolic mechanism that supplies energy under anoxic conditions. the fungus fusarium oxysporum reduces nitrate to ammonium and simultaneously oxidizes ethanol to acetate to generate atp (zhou, z., takaya, n., nakamura, a., yamaguchi, m., takeo, k., and shoun, h. (2002) j. biol. chem. 277, 1892-1896). we identified the aspergillus nidulans genes involved in ammonia fermentation by analyzing fungal mutants. the results showed that assimilatory ... | 2004 | 14722082 |
| a one-step method to convert vectors into binary vectors suited for agrobacterium-mediated transformation. | bacterial artificial chromosomes (bacs) are widely used for the construction of physical maps, positional-cloning and whole-genome sequencing strategies. unfortunately, their use for functional genomics is limited, as currently there is no efficient method to use bacs directly for complementation. we describe a novel strategy for one-step conversion of any bac into a binary bac (bibac). using agrobacterium tumefaciens, these bibacs can be efficiently transformed to virtually all organisms, inclu ... | 2004 | 14745506 |
| fusarium oxysporum as a multihost model for the genetic dissection of fungal virulence in plants and mammals. | fungal pathogens cause disease in plant and animal hosts. the extent to which infection mechanisms are conserved between both classes of hosts is unknown. we present a dual plant-animal infection system based on a single strain of fusarium oxysporum, the causal agent of vascular wilt disease in plants and an emerging opportunistic human pathogen. injection of microconidia of a well-characterized tomato pathogenic isolate (isolate 4287) into the lateral tail vein of immunodepressed mice resulted ... | 2004 | 14977985 |
| molecular mapping of fusarium oxysporum f. sp. ciceris race 3 resistance gene in chickpea. | sequence-tagged microsatellite site (stms) and sequence-tagged site (sts) markers linked closely to fusarium oxysporum f. sp. ciceris race 3 resistance gene in chickpea were identified, and linkage between three wilt resistance genes was elucidated. the resistance to race 3 in chickpea germplasm accession wr-315 was inherited as a single gene, designated foc-3, in 100 f(7) recombinant inbred lines derived from the cross of wr-315 (resistant) x c-104 (susceptible). the foc-3 gene was mapped 0.6 c ... | 2004 | 14689189 |
| cerebroside elicitor confers resistance to fusarium disease in various plant species. | abstract in the rice blast fungus pathosystem, cerebroside, a compound categorized as a sphingolipid, was found in our previous study to be a non-racespecific elicitor, which elicits defense responses in rice. here we describe that cerebroside c is produced in diverse strains of fusarium oxysporum, a common soilborne agent of wilt disease affecting a wide range of plant species. in addition, some type of cerebroside elicitor involving cerebroside a, b, or c was detected in other soilborne phytop ... | 2004 | 18943100 |
| fusarium foetens, a new species pathogenic to begonia elatior hybrids (begonia x hiemalis) and the sister taxon of the fusarium oxysporum species complex. | a new disease recently was discovered in begonia elatior hybrid (begonia × hiemalis) nurseries in the netherlands. diseased plants showed a combination of basal rot, vein yellowing and wilting and the base of collapsing plants was covered by unusually large masses of fusarium macroconidia. a species of fusarium was isolated consistently from the discolored veins of leaves and stems. it differed morphologically from f. begoniae, a known agent of begonia flower, leaf and stem blight. the fusarium ... | 2004 | 21148861 |
| stepwise evolution of races in fusarium oxysporum f. sp. ciceris inferred from fingerprinting with repetitive dna sequences. | abstract plant pathogens often exhibit variation in virulence, the ability to cause disease on host plants with specific resistance, evident from the diversity of races observed within pathogen species. the evolution of races in asexual fungal pathogens has been hypothesized to occur in a stepwise fashion, in which mutations to virulence accumulate sequentially in clonal lineages, resulting in races capable of overcoming multiple host plant resistance genes or multiple resistant cultivars. in th ... | 2004 | 18943970 |
| genetic diversity of pathogenic and nonpathogenic populations of fusarium oxysporum isolated from carnation fields in argentina. | abstract in order to elucidate the origin of fusarium oxysporum f. sp. dianthi in argentina, the genetic diversity among pathogenic isolates together with co-occurring nonpathogenic isolates on carnation was investigated. in all, 151 isolates of f. oxysporum were obtained from soils and carnation plants from several horticultural farms in argentina. the isolates were characterized using vegetative compatibility group (vcg), intergenic spacer (igs) typing, and pathogenicity tests on carnation. se ... | 2004 | 18943491 |
| effect of moisture on thermal inactivation of soilborne pathogens under structural solarization. | abstract structural solarization of greenhouses for sanitation by closing them involves dry heating to 60 degrees c and higher with a consequent low relative humidity (rh) ( approximately 15%), thus requiring an extended period for thermal inactivation of pathogens. in an attempt to enhance pathogen control by increasing moisture during the hot hours of the day, various regimes of inoculum moistening were studied. however, wetting inoculum of fusarium oxysporum f. sp. melonis and f. oxysporum f. ... | 2004 | 18943535 |
| quantification of root and stem colonization of watermelon by fusarium oxysporum f. sp. niveum and its use in evaluating resistance. | abstract colonization of watermelon root and stem tissues by fusarium oxysporum f. sp. niveum race 1 and its relationship to the apparent resistance to fusarium wilt was investigated. in each of 2 years, 17 differentially susceptible watermelon cultivars and one accession were tested in the greenhouse, and 7 cultivars also were tested in the field. colonization by a chlorate-resistant marked isolate of the fungus was assayed by plating homogenized tissue samples on a selective medium. six days a ... | 2004 | 18943103 |
| polygenic inheritance of partial resistance to fusarium oxysporum f. sp. melonis race 1.2 in melon. | abstract fusarium oxysporum f. sp. melonis is responsible for fusarium wilt of melon. race 1.2 strains overcome two dominant resistance genes (fom-1 and fom-2) and are further divided into two types depending on the symptoms they cause, yellowing or wilting. partial resistance to f. oxysporum f. sp. melonis race 1.2 was studied by using a recombinant inbred line (ril) population that was developed by single seed descent from an f(1) hybrid between 'isabelle', a partially resistant line, and a su ... | 2004 | 18943703 |
| integrated management of fusarium wilt of chickpea with sowing date, host resistance, and biological control. | abstract a 3-year experiment was conducted in field microplots infested with fusarium oxysporum f. sp. ciceris race 5 at córdoba, spain, in order to assess efficacy of an integrated management strategy for fusarium wilt of chickpea that combined the choice of sowing date, use of partially resistant chickpea genotypes, and seed and soil treatments with biocontrol agents bacillus megaterium rgaf 51, b. subtilis gb03, nonpathogenic f. oxysporum fo 90105, and pseudomonas fluorescens rg 26. advancing ... | 2004 | 18943071 |
| interaction of rotylenchulus reniformis with seedling disease pathogens of cotton. | the impact of 10 fusarium species in concomitant association with rotylenchulus reniformis on cotton seedling disease was examined under greenhouse conditions. in experiment 1, fungal treatments consisted of fusarium chlamydosporum, f. equiseti, f. lateritium, f. moniliforme, f. oxysporum, f. oxysporum f.sp. vasinfectum, f. proliferatum, f. semitectum, f. solani, and f. sporotrichioides; rhizoctonia solani; and thielaviopsis basicola. the experimental design was a 2 x 14 factorial consisting of ... | 2004 | 19262802 |
| high genetic diversity among strains of fusarium oxysporum f. sp. vasinfectum from cotton in ivory coast. | abstract seventeen isolates of fusarium oxysporum f. sp. vasinfectum from the ivory coast were characterized using vegetative compatibility group (vcg), restriction fragment length polymorphism of the ribosomal inter-genic spacer region (igs), and mating type (mat) idiomorph, and compared with a worldwide collection of the pathogen containing all available reference strains. some of the isolates were identical to known reference strains for all three traits, whereas others had previously unknown ... | 2005 | 18943549 |
| biological control efficiency of fusarium wilt of tomato by nonpathogenic fusarium oxysporum fo-b2 in different environments. | abstract efficiency of nonpathogenic fusarium oxysporum fo-b2 for the biological control of fusarium wilt of tomato, caused by f. oxysporum f. sp. lycopersici cu1, was examined in different environments: a growth chamber with sterile soil-less medium, a greenhouse with fumigated or nonfumigated soil, and nonfumigated field plots. inoculation of fo-b2 onto tomato roots significantly reduced the severity of disease, but the efficiency of disease suppression decreased as the experimental environmen ... | 2005 | 18943305 |
| foliar spray of validamycin a or validoxylamine a controls tomato fusarium wilt. | abstract tomato wilt, caused by the soilborne fungus fusarium oxysporum f. sp. lycopersici, is effectively controlled by a foliar spray of validamycin a (vma) or validoxylamine a (vaa) (>/=10 mug/ml); however, neither vma nor vaa is antifungal in vitro. in pot tests, the effect of a foliar application of vma or vaa at 100 mug/ml lasted for 64 days. plants sprayed with vma or vaa accumulated salicylic acid and had elevated expression of the systemic acquired resistance (sar) marker genes p4 (pr-1 ... | 2005 | 18943474 |
| effect of mixed and single crops on disease suppressiveness of soils. | abstract the effect of mixed cropping on disease suppressiveness of soils was tested for two cropping systems, brussels sprouts-barley and triticale-white clover. disease suppressiveness of field soils was evaluated in bioassays for the soilborne pathogens rhizoctonia solani, fusarium oxysporum f. sp. lini, and gaeumannomyces graminis var. tritici. for both cropping systems, mixed cropping did not enhance disease suppressiveness of the soils. in some cases, soil cropped to barley alone was signi ... | 2005 | 18943364 |
| development of crown and root rot disease of tomato under irrigation with saline water. | abstract we studied the effect of water salinity on the incidence and severity of crown and root rot disease of tomato, as well as on the pathogen and on the plant's response to the pathogen. irrigation with saline water significantly increased disease severity in tomato transplants inoculated with fusarium oxysporum f. sp. radicis-lycopersici, and mineral fertilization further increased it. in one field experiment, disease incidence in plots irrigated with saline water (electrical conductivity ... | 2005 | 18943555 |
| nitrogen-responsive genes are differentially regulated in planta during fusarium oxyspsorum f. sp. lycopersici infection. | summary nitrogen is an essential growth component whose availability will limit microbial spread, and as such it serves as a key control point in dictating an organism's adaptation to various environments. little is known about fungal nutrition in planta. to enhance our understanding of this process we examined the transcriptional adaptation of fusarium oxysporum f. sp. lycopersici, the causal agent for vascular wilt in tomato, during nutritional stress and plant colonization. using rt-pcr and m ... | 2005 | 20565671 |
| effect of iron availability on induction of systemic resistance to fusarium wilt of chickpea by pseudomonas spp. | selected isolates of pseudomonas fluorescens (pf4-92 and pfrsc5) and p. aeruginosa (parsg18 and parsg27) were examined for growth promotion and induced systemic resistance against fusarium wilt of chickpea. significant increase in plant height was observed in pseudomonas treated plants. however, plant growth was inhibited when isolates of pseudomonas were used in combination with fusarium oxysporum f. sp. ciceri (focrs1). it was also observed that the pseudomonas spp. was colonized in root of ch ... | 2005 | 24049472 |
| pathogenicity of two species of fusarium on some cultivars of bean in greenhouse. | twenty isolates of fusarium oxysporum and f. solani were isolated from the infected roots of bean in different farms of east azarbaijan and tehran provinces and their pathogenicity determined. most isolates of the fungi were identified as f. oxysporun. they caused root rot, yellowing and wilting of bean in the field. in this test, the roots of 6 cultivars of bean seedlings soaked in suspension of the 7 isolates of the fungi (a1, gogan, a2, bilverdi, a3, savojbolagh-hashtgerd, a4, field of agr. c ... | 2005 | 16637191 |
| suppression of fusarium wilt by combining green compost and trichoderma hamatum. | fusarium wilts, caused by the fungus fusarium oxysporum, are important diseases of horticultural and agricultural crops and lead to significant yield losses. the pathogen infects the roots and colonizes the vascular tissue, leading to wilting and finally death of the plant. the objective of this study was to investigate the efficacy of amendment of green compost and a trichoderma hamatum strain against fusarium wilt of radish. the substrate effects and the effect of a trichoderma strain were tes ... | 2005 | 16637174 |
| fungi and nematodes occurring in different composts used in uruguayan agriculture. | forty three thermophilic, thermotolerant and mesophilic fungal species and nematodes from five trophic groups were found in four compost types. aspergillus fumigatus and a. niger were the most frequent species. plant pathogenic fungi viz. fusarium oxysporum, f. acuminatum, f. nygamai and f. solani were found in some of the composts in low frequency. the most abundant species of nematode corresponded to bacterivores. tylenchus, a phytophagous nematode was scarcely found. | 2005 | 16459545 |
| biotic relation between fusarium oxysporum schlecht. and fungi isolated from the substrate of stewartia pseudocamellia (max.). | the effect of the fungi community colonizing the substrate of stewartia plants on the growth of fusarium oxysporum schlecht. was investigated. the soil samples from 2 years old stewartia cuttings were taken for analysis in the second decade of october 2002 and 2003, when the symptoms of disease appeared on stewartia plant. fungi were isolated from the substrate using the sand method (mańka k. 1974). fusarium oxysporum were isolated from root system of stewartia pseudocamellia plant. the isolates ... | 2005 | 16637175 |
| an antifungal peptide from the coconut. | a chromatographic procedure consisting of ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on cm-cellulose, and gel filtration by fast performance liquid chromatography on supedex 75 was utilized to isolate a 10 kda antifungal peptide from coconut flesh. the peptide was unadsorbed on deae-cellulose, but adsorbed on affi-gel blue gel and cm-cellulose. it displayed antifungal activity against fusarium oxysporum, mycosphaerella ... | 2005 | 16308082 |
| force calculations in automated docking: enzyme-substrate interactions in fusarium oxysporum cel7b. | autodock is a small-molecule docking program that uses an energy function to score docked ligands. here autodock's grid-based method for energy evaluation was exploited to evaluate the force exerted by fusarium oxysporum cel7b on the atoms of docked cellooligosaccharides and a thiooligosaccharide substrate analog. coupled with the interaction energies evaluated for each docked ligand, these forces give insight into the dynamics of the ligand in the active site, and help to elucidate the relative ... | 2005 | 16138313 |
| chemical composition and antioxidant, antimicrobial, and antifungal activities of the essential oil of achillea ligustica all. | the chemical composition of the essential oil from flowering tops of achillea ligustica all. was studied. samples were collected in different localities of sardinia (italy) and hydrodistilled both with clevenger-type and with simultaneous distillation-extraction apparatus. the yields ranged between 0.88 +/- 0.06 and 0.43 +/- 0.02% (vol/dry wt). the essential oils were analyzed by gc-ms, and a total of 96 components were detected. from a qualitative point of view, irrelevant differences between s ... | 2005 | 16366708 |
| the sen1 gene of arabidopsis is regulated by signals that link plant defence responses and senescence. | plant defence and senescence share many similarities as evidenced by extensive co-regulation of many genes during these responses. to better understand the nature of signals that are common to plant defence and senescence, we studied the regulation of sen1 encoding a senescence-associated protein during plant defence responses in arabidopsis. pathogen inoculations and treatments with defence-related chemical signals, salicylic acid and methyl jasmonate induced changes in sen1 transcript levels. ... | 2005 | 16325410 |
| gibberella xylarioides sensu lato from coffea canephora: a new mating population in the gibberella fujikuroi species complex. | gibberella xylarioides heim & saccas (presumed anamorph, fusarium xylarioides steyaert) is the causal agent of coffee wilt disease, an economically important tracheomycosis in africa. in vitro crosses carried out with congolese, ugandan, and tanzanian single-ascospore/conidial isolates originating from diseased coffea canephora/excelsa demonstrated a heterothallic mating system, controlled by a single locus with two alleles, mat-1 and mat-2. compatible isolates produced fertile perithecia within ... | 2005 | 16332836 |
| onychomycosis in children: a survey of 46 cases. | this is a retrospective study of the agents, clinical aspects, sources of infection and therapy of onychomycosis in children. in the period 1989-2000, we observed 46 consecutive children, until 16 years of age with onychomycosis (29 boys, 17 girls, mean age 10.8 years). dermatophytes were isolated in 30 cases (trichophyton rubrum in 22 cases, trichophyton mentagrophytes in five, epidermophyton floccosum in two and trichophyton violaceum in one) and candida spp. in 16, associated with trichophyto ... | 2005 | 16262881 |
| [ecological effects of multifunctional micro-flora agent in environment]. | in this paper, strains zjy-1 (brevibacillus brevis) and zjy-116 (bacillus subtilis) with evident inhibitory effect on fusarium oxysporum f. sp. cucumerinum and with promotion effect on plant growth were screened from cucumber rhizosphere, and the feasibility of exerting the ecological effects of multifunctional micro-flora agent was studied by treating cucumber seeds with the two strains singly or mixed with chlorpyrifos-degrading strain dsp3 (alcaligenes faecali). the results showed that the tw ... | 2005 | 16422513 |
| variability of non-mutualistic filamentous fungi associated with atta sexdens rubropilosa nests. | a survey of the filamentous fungi other than the symbiotic one found in association with atta sexdens rubropilosa colonies was carried out. different fungal species (27 taxa) were isolated a few days after treating the workers with toxic baits (sulfluramid; mirex-s), from 40 laboratory and 20 field nests. syncephalastrum racemosum (54%) and escovopsis weberi (21%), trichoderma harzianum (38%) and fusarium oxysporum (23%) were the prevalent species in laboratory and field nests, respectively. acr ... | 2005 | 16475502 |
| rapid differentiation of fusarium oxysporum isolates using pcr-sscp with the combination of ph-variable electrophoretic medium and low temperature. | differentiation of fusarium oxysporum is significantly important for unraveling the pathogenetic mechanism of fusaria wilts. in this study, isolates of f. oxysporum were screened from the soils in the rhizosphere of watermelon plant by komada medium and differentiated by sscp approach with the combination of ph-variable electrophoretic medium (tris-mes-edta (tme), ph 6.1) and low temperature (9 degrees c). we found that tme was a good electrophoretic medium and its ph value was variable over the ... | 2005 | 16287185 |
| [heterologous expression and functional identification of a chitinase gene from wheat]. | chitinases are functional in plant defense against pathogens and also involved in plant development as well as human disease responses. in this study a chitinase gene, wch2, from wheat was expressed transiently in tobacco leaves and stably in transgenic arabidopsis thaliana plants to evaluate its heterologous expression and potential application. western blot analysis revealed that the expressed wheat chitinase was about 30 kd as was expected (fig.1) and had hydrolytic activity towards chitin po ... | 2005 | 16361785 |