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rapid synthesis of silver nanoparticles from fusarium oxysporum by optimizing physicocultural conditions.synthesis of silver nanoparticles (snps) by fungi is emerging as an important branch of nanotechnology due to its ecofriendly, safe, and cost-effective nature. in order to increase the yield of biosynthesized snps of desired shape and size, it is necessary to control the cultural and physical parameters during the synthesis. we report optimum synthesis of snps on malt extract glucose yeast extract peptone (mgyp) medium at ph 9-11, 40-60°c, and 190.7 lux and in sun light. the salt concentrations, ...201324222751
selection for fusarium wilt disease resistance from regenerants derived from leaf callus of strawberry.resistant lines of strawberry to the fungal wilt disease caused by fusarium oxysporum f. sp. fragariae were selected strawberry plants regenerated from leaf-derived callus tissues. regenerants were transplanted to a field heavily infested with this pathogen, and normally growing plants were selected as the putative resistant lines. daughter plants produced vegetatively through runner formation of the lines were similarly tested in the pathogen-infested field over an additional three generations. ...199124221538
somaclonal variant uc-t3: the expression of fusarium wilt resistance in progeny arrays of celery, apium graveolens l.first generation (s1) progeny, second generation (s2) progeny, and backcross (bc) progeny of a celery (apium graveolens l. var. dulce) somaclonal variant, uc-t3, were evaluated for resistance to the fungus fusarium oxysporum f. sp. apii, race 2 (foa2). chisquare analysis of s1 progeny showed that the expression of resistance did not fit a single-locus model. s2 progeny means were similar among families, except in a heavily infested field. the lowest ranking s2 family in both the lightly infested ...199024220975
rohitukine, a chromone alkaloid and a precursor of flavopiridol, is produced by endophytic fungi isolated from dysoxylum binectariferum hook.f and amoora rohituka (roxb).wight & arn.rohitukine, a chromone alkaloid, has gained considerable international attention in recent years because of its novel semi-synthetic derivative, flavopiridol and p-276-00. both these molecules are in advanced stages of clinical development and trial for cancer treatment. recently, flavopiridol was approved as an orphan drug for treatment of chronic lymphocytic leukemia cancer. the natural occurrence of rohitukine is restricted to only four plant species, amoora rohituka and dysoxylum binectarife ...201424215673
rflp mapping of i1, a new locus in tomato conferring resistance against fusarium oxysporum f. sp. lycopersici race 1.the inheritance and linkage relationships of a gene for resistance to fusarium oxysporum f. sp. lycopersici race 1 were analyzed. an interspecific hybrid between a resistant lycopersicon pennellii and a susceptible l. esculentum was backcrossed to l. esculentum. the genotype of each backcross-1 (bc1) plant with respect to its fusarium response was determined by means of backcross-2 progeny tests. resistance was controlled by a single dominant gene, i1, which was not allelic to i, the traditional ...199124212856
interactions between the entomopathogenic nematode heterorhabditis sonorensis (nematoda: heterorhabditidae) and the saprobic fungus fusarium oxysporum (ascomycota: hypocreales).in this study, we assessed the effect of the saprobic fungus, fusarium oxysporum (ascomycota: hypocreales) on the fitness of the entomopathogenic nematode heterorhabditis sonorensis (caborca strain). sand column assays were considered to evaluate the effect of fungal mycelia on infective juvenile (ij) movement and host access. additionally, we investigated the effect of fungal spores on the nematodes' ability to search for a host, its virulence, penetration efficiency and reproduction. three app ...201424211424
the in vitro physiological phenotype of tomato resistance to fusarium oxysporum f. sp. lycopersici.with the aim of dissecting host-parasite interaction processes in the system lycopersicon aesculentum-fusarium oxysporum f. sp. lycopersici we have isolated plant cell mutants having single-step alterations in their defense response. a previous analysis of the physiological phenotypes of mutant cell clones suggested that recognition is the crucial event for active defence, and that polysaccharide content, fungal growth inhibition, peroxidase induction in in vitro dual culture and ion leakage ind ...199224203038
time-course study of the accumulation of hydroxyproline-rich glycoproteins in root cells of susceptible and resistant tomato plants infected by fusarium oxysporum f. sp. radicis-lycopersici.the accumulation of hydroxyproline-rich glycoproteins (hrgps) in cell walls of dicotyledonous plants is thought to be involved in the defense response to pathogens. an antiserum raised against deglycosylated hrgps from melon was used for studying the subcellular localization of these glycoproteins in susceptible and resistant tomato (lycopersicon esculentum mill.) root tissues infected by fusarium oxysporum f.sp. radicis-lycopersici. a time-course of hrgp accumulation revealed that these glycopr ...199124194071
biochemical characterization of fruit-specific pathogenesis-related antifungal protein from basrai banana.pathogenesis-related/thaumatin like (pr-5/tl) antifungal protein from basrai banana was purified by using a simple protocol consisting of ammonium sulphate precipitation, affinity chromatography (affi-gel blue gel), q-sepharose chromatography and gel filtration on sephadex g-75. the purified protein with acidic character (pi 6.67) has molecular weight of 21.155 kda, as determined by maldi-tof mass spectrometry. the purified protein shared n-terminal sequence homology with other tlps. crude banan ...201424192113
modification of competence for in vitro response to fusarium oxysporum in tomato cells. i. selection from a susceptible cultivar for high and low polysaccharide content.plant cell walls play a major role in the outcome of host-parasite interactions. wall fragments released from the plant, and/or the fungal pathogen, can act respectively as endogenous and exogenous elicitors of the defence response, and other wall components, such as callose, lignin, or hydroxyproline-rich glycoproteins, can inhibit pathogen penetration and/or spreading. we have previously demonstrated that calli from tomato cultivars resistant in vivo to fusarium oxysporum f.sp. lycopersici sho ...199424190533
modification of competence for in vitro response to fusarium oxysporum in tomato cells. ii. effect of the integration of agrobacterium tumefaciens genes for auxin and cytokinin synthesis.we have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (lycopersicon esculentum) cells to defend themselves against the fungal pathogen fusarium oxysporum f. sp. lycopersici. calluses from cvs 'davis' and 'red river', respectively resistant and susceptible to fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from agrobacterium tumefaciens, were used. the integration of agrobacterium hormone-related genes into susceptible cv 'red ...199424185887
a streptomyces chitosanase is active in transgenic tobacco.growth inhibition towards rhizopus nigricans, fusarium oxysporum f. sp. radicis-lycopersici, verticillium albo-atrum and pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, streptomyces sp, strain n174. the corresponding gene, with its own signal peptide, was inserted into pbi121.7 shuttle vector to transform tobacco. transgenic plants were analysed for chitosanase activity by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis assay. two major and on ...199524185780
synthesis and antifungal properties of (4-tolyloxy)-pyrimidyl-α-aminophosphonates chitosan derivatives.a novel class of α-aminophosphonate chitosan derivatives was investigated. these chitosan derivatives consist of (4-tolyloxy)-pyrimidyl-dimethyl-α-amino-phosphonate chitosan (α-atpmcs) and (4-tolyloxy)-pyrimidyl-diethyl-α-aminophosphonate chitosan (α-atpecs). their structures were well defined. antifungal activity of them against some crop-threatening pathogenic fungi was tested in vitro. the derivatives were found to have a broad-spectrum antifungal activity that was obviously enhanced compared ...201424183805
routine mapping of fusarium wilt resistance in bc(1) populations of arabidopsis thaliana.susceptibility to fusarium wilt disease varies among wild accessions of arabidopsis thaliana. six resistance to fusarium oxysporum (rfo) quantitative trait loci (qtls) controlling the resistance of accession columbia-0 (col-0) and susceptibility of taynuilt-0 to fusarium oxysporum forma specialis matthioli (fom) are detected in a recombinant population derived from a single backcross of the f1 hybrid (bc1). in particular, the rfo1 qtl appears to interact with three other loci, rfo2, rfo4 and rfo ...201324172069
antifungal cyclic lipopeptides from bacillus amyloliquefaciens strain bo5a.a bioassay-guided fractionation of bacillus amyloliquefaciens strain bo5a afforded the isolation of two new cyclic lipopeptides (1 and 2) as the major lipid constituents (>60%) of the chcl3-meoh (2:1) extract. the chemical structures of the isolated metabolites were elucidated by spectroscopic methods, including 1d and 2d nmr spectroscopy, mass spectrometry (ms), secondary ion mass spectrometry (ms1, ms2), and chemical degradation. the compounds are members of the surfactins family and are based ...201324164115
biofilm formation by fusarium oxysporum f. sp. cucumerinum and susceptibility to environmental stress.to the authors' knowledge, most studies on biofilm formation have focused on bacteria and yeasts. so far, biofilm formation by fungal plant pathogen has not been reported. in this study, the biofilm-forming capacity of fusarium oxysporum f. sp. cucumerinum was evaluated. for biofilm quantification, a colorimetric 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2h-tetrazolium-hydroxide (xtt) reduction assay was used to observe metabolic activity. fluorescence and confocal scann ...201424164057
generating phenotypic diversity in a fungal biocatalyst to investigate alcohol stress tolerance encountered during microbial cellulosic biofuel production.consolidated bioprocessing (cbp) of lignocellulosic biomass offers an alternative route to renewable energy. the crop pathogen fusarium oxysporum is a promising fungal biocatalyst because of its broad host range and innate ability to co-saccharify and ferment lignocellulose to bioethanol. a major challenge for cellulolytic cbp-enabling microbes is alcohol inhibition. this research tested the hypothesis that agrobacterium tumefaciens--mediated transformation (atmt) could be exploited as a tool to ...201324147009
antifungal activity of microgramma vacciniifolia rhizome lectin on genetically distinct fusarium oxysporum f. sp. lycopersici races.fusarium oxysporum f. sp. lycopersici races 1, 2, and 3 deteriorate tomato crops since they cause a vascular wilt. lectins are carbohydrate-binding proteins with hemagglutinating and antifungal activities. this work reports that microgramma vacciniifolia rhizome lectin (mvrl) inhibits f. oxysporum f. sp. lycopersici race 3 growth (61 %) more intensely than of races 1 (55 %) and 2 (45 %). the hemagglutinating activity of mvrl was inhibited by glycoprotein preparations from mycelia of races 1, 2, ...201424142386
insights from the draft genome of paenibacillus lentimorbus nrrl b-30488, a promising plant growth promoting bacterium.paenibacillus lentimorbus nrrl b-30488, a plant growth-promoting bacterium was isolated from sahiwal cow's milk. the strain shows antagonism against phytopathogens, fusarium oxysporum f. sp. ciceri and alternaria solani. its genome contains gene clusters involved in nonribosomal synthesis of secondary metabolites involved in antimicrobial activities. the genome sequence of p. lentimorbus nrrl b-30488 provides the genetic basis for application of this bacterial strain in plant growth promotion, p ...201324140292
protein extraction and gel-based separation methods to analyze responses to pathogens in carnation (dianthus caryophyllus l).we are currently using a 2-de-based proteomics approach to study plant responses to pathogenic fungi by using the carnation (dianthus caryophyllus l)-fusarium oxysporum f. sp. dianthi pathosystem. it is clear that the protocols for the first stages of a standard proteomics workflow must be optimized to each biological system and objectives of the research. the optimization procedure for the extraction and separation of proteins by 1-de and 2-de in the indicated system is reported. this strategy ...201424136548
exopolysaccharides and antimicrobial biosurfactants produced by paenibacillus macerans tku029.paenibacillus macerans tku029 can produce exopolysaccharides (epss; 3.46 g/l) and a biosurfactant (1.78 g/l) in a medium with 2 % (w/v) squid pen powder as the sole carbon/nitrogen source. the biosurfactant can reduce the surface tension of water from 72.30 to 35.34 mn/m at a concentration of 2.76 g/l and reach an emulsification index of 56 % after a 24-h reaction with machine oil. this biosurfactant is stable at 121 °c for 20 min, over a ph range from 3 to 11, and in <5 % salt solutions. it als ...201424122708
long noncoding rnas responsive to fusarium oxysporum infection in arabidopsis thaliana.short noncoding rnas have been demonstrated to play important roles in regulation of gene expression and stress responses, but the repertoire and functions of long noncoding rnas (lncrnas) remain largely unexplored, particularly in plants. to explore the role of lncrnas in disease resistance, we used a strand-specific rna-sequencing approach to identify lncrnas responsive to fusarium oxysporum infection in arabidopsis thaliana. antisense transcription was found in c. 20% of the annotated a. thal ...201424117540
induced resistance to botrytis cinerea in capsicum annuum by a fusarium crude elicitor fraction, free of proteins.fusarium oxysporum f. sp. lycopersici (fol) induces resistance in pepper against the airborne pathogen botrytis cinerea and the soil-borne pathogen verticillium dahliae. however, its practical use is limited due to its pathogenicity to other crops. in this study we tested several fractions of a heat-sterilised crude fol-elicitor preparation to protect pepper against b. cinerea and v. dahliae. only the protein-free insoluble fraction of the preparation reduced b. cinerea infection. however, none ...201324112636
transcriptome and expression profile analysis of highly resistant and susceptible banana roots challenged with fusarium oxysporum f. sp. cubense tropical race 4.banana wilt disease, caused by the fungal pathogen fusarium oxysporum f. sp. cubense 4 (foc4), is regarded as one of the most devastating diseases worldwide. cavendish cultivar 'yueyoukang 1' was shown to have significantly lower disease severity and incidence compared with susceptible cultivar 'brazilian' in greenhouse and field trials. de novo sequencing technology was previously performed to investigate defense mechanism in middle resistant 'nongke no 1' banana, but not in highly resistant cu ...201324086302
discovery of a linoleate 9s-dioxygenase and an allene oxide synthase in a fusion protein of fusarium oxysporum.fusarium oxysporum is a devastating plant pathogen that oxidizes c₁₈ fatty acids sequentially to jasmonates. the genome codes for putative dioxygenase (dox)-cytochrome p450 (cyp) fusion proteins homologous to linoleate diol synthases (ldss) and the allene oxide synthase (aos) of aspergillus terreus, e.g., foxb_01332. recombinant foxb_01332 oxidized 18:2n-6 to 9s-hydroperoxy-10(e),12(z)-octadecadienoic acid by hydrogen abstraction and antarafacial insertion of molecular oxygen and sequentially to ...201324082064
crude extract of fusarium oxysporum induces apoptosis and structural alterations in the skin of healthy rats.we evaluate the biological and physicochemical effects of a fusarium oxysporum crude extract (ce) on the skin of healthy rats. the ce is topically applied and subsequently the skin is collected after 3, 6, 12, and 24 h. the samples are analyzed by fourier transform infrared photoacoustic spectroscopy (ftir-pas) and histomorphometric analysis. terminal dutp nick end labeling (tunel assay) is performed to detect both the cells in apoptosis and proliferation. there is a thickening of the epidermis ...201324077666
integrated management strategies for tomato fusarium wilt.fusarium wilt is caused by the fungal pathogens, fusarium oxysporum or fusarium solani. it is a devastating disease that affects many important food and vegetable crops and a major source of loss to farmers worldwide. initial strategies developed to combat this devastating plant disease include the use of cultural, physical and chemical control. none of these strategies have been able to give the best results of completely ameliorating the situation except for the cultural method which is mainly ...201324077535
synthesis and bioactivities of novel thioether/sulfone derivatives containing 1,2,3-thiadiazole and 1,3,4-oxadiazole/thiadiazole moiety.a series of new thioether/sulfone compounds containing 1,2,3-thiadiazole and 1,3,4-oxadiazole/1,3,4-thiadiazole moiety were synthesized, the structures of all products were confirmed by ir, (1)h nmr, (13)c nmr, and element analysis. preliminary antifungal activity test showed that compound 8a exhibited moderate antifungal activity against fusarium oxysporum at 50μg/ml. preliminary antiviral activity results showed that compounds 7a, 7c, 7d, 8a, and 9a displayed high antiviral activity against to ...201324070781
proteomic analysis of fusarium oxysporum f. sp. cubense tropical race 4-inoculated response to fusarium wilts in the banana root cells.fusarium wilt of banana is one of the most destructive diseases in the world. this disease has caused heavy losses in major banana production areas. except for molecular breeding methods based on plant defense mechanisms, effective methods to control the disease are still lacking. dynamic changes in defense mechanisms between susceptible, moderately resistant, and highly resistant banana and fusarium oxysporum f. sp. cubense tropical race 4 (foc4) at the protein level remain unknown. this resear ...201324070062
isolation, purification and characterization of vinblastine and vincristine from endophytic fungus fusarium oxysporum isolated from catharanthus roseus.endophytic fungi reside in a symbiotic fashion inside their host plants, mimic their chemistry and interestingly, produce the same natural products as their hosts and are thus being screened for the production of valuable compounds like taxol, camptothecin, podophyllotoxin, etc. vinblastine and vincristine are excellent anti-cancer drugs but their current production using plants is non-abundant and expensive. in order to make these drugs readily available to the patients at affordable prices, we ...201324066024
fusarium oxysporum f.sp. ciceri race 1 induced redox state alterations are coupled to downstream defense signaling in root tissues of chickpea (cicer arietinum l.).reactive oxygen species are known to play pivotal roles in pathogen perception, recognition and downstream defense signaling. but, how these redox alarms coordinate in planta into a defensive network is still intangible. present study illustrates the role of fusarium oxysporum f.sp ciceri race1 (foc1) induced redox responsive transcripts in regulating downstream defense signaling in chickpea. confocal microscopic studies highlighted pathogen invasion and colonization accompanied by tissue damage ...201324058463
microwave assisted synthesis of dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones; synthesis, in vitro antimicrobial and anticancer activities of novel coumarin substituted dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones.the present article describes the synthesis of dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-one (2a-h) under microwave irradiation. the product was obtained in excellent yield (74-94%) in a shorter reaction time (2 min). these molecules (2a, b) further reacted with various substituted 4-bromomethylcoumarins (3a-f) to yield a new series of coumarin substituted dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones (4a-h). the structure of all the synthesized compounds were confirmed by spectral studies an ...201324056147
spectroscopic and biological approach in the characterization of a novel 14-membered [n4] macrocyclic ligand and its palladium(ii), platinum(ii), ruthenium(iii) and iridium(iii) complexes.a novel, tetradentate nitrogen donor [n4] macrocyclic ligand, i.e. 3,5,14,16-tetramethyl-2,6,13,17-tetraazatricyclo[12,0,0(7-12)] cosa-1(22),2,5,7,9,11,13,16,18,20-decaene(l), has been synthesized and characterized by elemental analyses, ir, mass, and (1)h nmr spectral studies. complexes of pd(ii), pt(ii), ru(iii) and ir(iii) have been prepared and characterized by elemental analyses, molar conductance measurements, magnetic susceptibility measurements, ir, mass, electronic spectral and thermal ...201424051297
increased incidence of invasive fusariosis with cutaneous portal of entry, brazil.invasive fusariosis (if) is an infection with fusarium spp. fungi that primarily affects patients with hematologic malignancies and hematopoietic cell transplant recipients. a cutaneous portal of entry is occasionally reported. we reviewed all cases of if in brazil during 2000-2010, divided into 2 periods: 2000-2005 (period 1) and 2006-2010 (period 2). we calculated incidence rates of if and of superficial infections with fusarium spp. fungi identified in patients at a dermatology outpatient uni ...201324050318
effect of iron availability on induction of systemic resistance to fusarium wilt of chickpea by pseudomonas spp.selected isolates of pseudomonas fluorescens (pf4-92 and pfrsc5) and p. aeruginosa (parsg18 and parsg27) were examined for growth promotion and induced systemic resistance against fusarium wilt of chickpea. significant increase in plant height was observed in pseudomonas treated plants. however, plant growth was inhibited when isolates of pseudomonas were used in combination with fusarium oxysporum f. sp. ciceri (focrs1). it was also observed that the pseudomonas spp. was colonized in root of ch ...200524049472
comparison of rapd, aflp, and ef-1α sequences for the phylogenetic analysis of fusarium oxysporum and its formae speciales in korea.although fursarium oxysporum causes diseases in economically important plant hosts, identification of f. oxysporum formae speciales has been difficult due to confusing phenotypic classification systems. to resolve these complexity, we evaluated genetic relationship of nine formae speciales of f. oxysporum with random amplified polymorphic dna (rapd), amplified fragment length polymorphism (aflp), and translation elongation factor-1 alpha (ef-1α) gene. in addition, the correlation between mycotox ...200624039470
fusarium keratitis in brazil: genotyping, in vitro susceptibilities, and clinical outcomes.the purpose of this paper is to describe clinical characteristics and determine correlations between clinical outcomes and antifungal susceptibility among molecularly characterized ocular fusarium isolates in brazil.201324039389
surveillance of bacterial and fungal infections in the postoperative period following liver transplantation: a series from 2005-2011.orthotopic liver transplantation (olt) is a life-saving procedure for the treatment of many end-stage diseases, but infectious and acute rejection episodes remain major causes of morbidity and mortality. bacterial and fungal infections can be due to intra-abdominal, biliary, respiratory, urinary, wound, central venous catheters (cvc) or unknown sources. using the computerized database of our microbiology laboratory, we analyzed all the bacterial and fungal infections in the first three months fo ...201324034031
evaluation of antifungal activity in essential oil of the syzygium aromaticum (l.) by extraction, purification and analysis of its main component eugenol.antifungal properties of some essential oils have been well documented. clove oil is reported to have strong antifungal activity against many fungal species. in this study we have evaluated antifungal potential of essential oil of syzygium aromaticum (l.) against some common fungal pathogens of plants and animals namely, fusarium moniliforme ncim 1100, fusarium oxysporum mtcc 284, aspergillus sp., mucor sp., trichophyton rubrum and microsporum gypseum. all fungal species were found to be inhibit ...201124031751
inoculation of tomato seedlings with trichoderma harzianum and arbuscular mycorrhizal fungi and their effect on growth and control of wilt in tomato seedlings.a green house study was conducted to investigate the ability of an isolate of trichoderma harzianum (p52) and arbuscular mycorrhizal fungi (amf) in enhancing growth and control of a wilt pathogen caused by fusarium oxysporum f. sp. lycopersici in tomato seedlings. the plants were grown in plastic pots filled with sterilized soils. there were four treatments applied as follows; p52, amf, amf + p52 and a control. a completely randomized design was used and growth measurements and disease assessmen ...201124031662
biological activities of a mixture of biosurfactant from bacillus subtilis and alkaline lipase from fusarium oxysporum.in this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from bacillus subtilis and an alkaline lipase from fusarium oxysporum (al/bs mix) on several types of microorganisms, as well as their abilities to remove listeria innocua atcc 33093 biofilm from stainless steel coupons. the al/bs mix had a surface tension of around 30 mn.m(-1), indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component. t ...201124031642
identification of an antifungal metabolite produced by a potential biocontrol actinomyces strain a01.actinomyces strain a01 was isolated from soil of a vegetable field in the suburb of beijing, china. according to the morphological, cultural, physiological and biochemical characteristics, and 16s rdna sequence analysis, strain a01 was identified as streptomyces lydicus. in the antimicrobial spectrum test strain a01 presented a stable and strong inhibitory activity against several plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, monilinia laxa, etc. however, no antibacterial ...200824031293
inoculum padronization for the production of cutinase by fusarium oxysporum.cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. the widespread use of this enzyme depends on the development of an efficient and low-cost production system. one of the most important steps in a fermentation process is the standardization of the inoculum characteristics. in this study, the production of cutinase by fusarium oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum pda ph. t ...200824031183
synthesis and bioactivity evaluation of novel arylimines containing a 3-aminoethyl-2-[(p-trifluoromethoxy)anilino]-4(3h)-quinazolinone moiety.twenty-seven novel (e)-3-[2-arylideneaminoethyl]-2-[4-(trifluoromethoxy)anilino]-4(3h)-quinazolinone derivatives were synthesized by reacting various aromatic aldehydes with intermediate 6. the target compounds were characterized by (1)h nmr, (3)c nmr, ir, and elemental analysis. bioassay results revealed that some of the compounds have strong antifungal activities against six fungi ( gibberella zeae , fusarium oxysporum , clematis mandshurica , paralepetopsis sasakii , phytophthora infestans , ...201324028303
isolation, evaluation and characterization of bacillus subtilis from cotton rhizospheric soil with biocontrol activity against fusarium oxysporum.present investigation is based on the isolation of bacillus subtilis from cotton rhizosphere and their evaluation as biocontrol agent against fusarium oxysporum. the production of extracellular hydrolytic enzyme was studied for determining the antagonism. 43% of 21 isolates were identified under the b. subtilis group on the basis of biochemical characterization. 38% isolates showed competitive activity against fusarium oxysporum and exhibit more than 50% mycelial inhibition in dual culture bioas ...201024026922
fusarium pathogenomics.fusarium is a genus of filamentous fungi that contains many agronomically important plant pathogens, mycotoxin producers, and opportunistic human pathogens. comparative analyses have revealed that the fusarium genome is compartmentalized into regions responsible for primary metabolism and reproduction (core genome), and pathogen virulence, host specialization, and possibly other functions (adaptive genome). genes involved in virulence and host specialization are located on pathogenicity chromoso ...201324024636
tandem combination of trigonella foenum-graecum defensin (tfgd2) and raphanus sativus antifungal protein (rsafp2) generates a more potent antifungal protein.plant defensins are small (45 to 54 amino acids) positively charged antimicrobial peptides produced by the plant species, which can inhibit the growth of a broad range of fungi at micro-molar concentrations. these basic peptides share a common characteristic three-dimensional folding pattern with one α-helix and three β-sheets that are stabilized by eight disulfide-linked cysteine residues. instead of using two single-gene constructs, it is beneficial when two effective genes are made into a sin ...201324022215
inhibition of cereal rust fungi by both class i and ii defensins derived from the flowers of nicotiana alata.defensins are a large family of small, cysteine-rich, basic proteins, produced by most plants and plant tissues. they have a primary function in defence against fungal disease, although other functions have been described. this study reports the isolation and characterization of a class i secreted defensin (nad2) from the flowers of nicotiana alata, and compares its antifungal activity with the class ii defensin (nad1) from n. alata flowers, which is stored in the vacuole. nad2, like all other c ...201424015961
characterization of fusarium oxysporum isolated from paprika in korea.in the present study we first report in korea the identification and characterization of fusarium oxysporum isolated from rotten stems and roots of paprika (capsicum annuum var. grossum) at masan, kyungsangnamdo in 2006. the fungal species produced white aerial mycelia accompanying with dark violet pigment on pda. the optimal temperature and ph for the growth of the species was 25℃ and ph 7, respectively. microscopic observation of one of isolates of the species shows that its conidiophores are ...200724015078
[bletilla striata pseudobulb rot pathogen identification and its biological characteristic].to identify the pathogen of bletilla striata pseudobulb rot and provide foundation for formulation effective control measures.201324010310
inhibitory effect of algal extracts on mycelial growth of the tomato-wilt pathogen, fusarium oxysporum f. sp. lycopersici.the present study was undertaken to explore the inhibitory effect of cyanobacterial extracts of nostoc commune fa-103 against the tomato-wilt pathogen, fusarium oxysporum f. sp. lycopersici. in an optimal medium, cell growth, antifungal activity, and antifungal compound production could be increased 2.7-fold, 4.1-fold, and 13.4-fold, respectively. a crude algal extract had a similar effect as mancozeb at the recommended dose, both in laboratory and pot tests. in vitro and in vivo fungal growth, ...200823997634
effect of some food preservatives on the lipolytic activity of beef luncheon fungi.beef luncheon meat is one of the most popular meals in several countries in the world including egypt. thirty one fungal species and 3 species varieties were recovered from 30 samples of beef luncheon meat collected from different supermarkets in qena. alternaria, aspergillus, emericella, mucor, mycosphaerella, penicillium and rhizopus were the most common genera on the two types of media. from the above genera, the most prevalent species were alternaria alternate, aspergillus flavus, a. fumigat ...200823997619
ecological and physiological studies on soil fungi at western region, libya.sixty three species and 5 varieties belonging to 30 fungal genera were collected from 75 soil samples. cultivated (29 genera and 58 species + 5 var.), desert (22 and 35 + 2 var.) and saline soil (21 and 41 + 1 var.) fungi were recovered on glucose-, cellulose- and 50% sucrose-czapek's agar at 28℃. the most common genera were alternaria, aspergillus, emericella, fusarium, mycosphaerella, nectria and penicillium. the most prevalent species from the three types of soils on the three types of media ...200823997599
molecular detection and genotyping of fusarium oxysporum f. sp. psidii isolates from different agro-ecological regions of india.twenty one isolates of fusarium oxysporum f. sp. psidii (fop), causing a vascular wilt in guava (psidium guajava l.), were collected from different agro-ecological regions of india. the pathogenicity test was performed in guava seedlings, where the fop isolates were found to be highly pathogenic. all 21 isolates were confirmed as f. oxysporum f. sp. psidii by a newly developed, species-specific primer against the conserved regions of 28s rdna and the intergenic spacer region. rapd and pcr-rflp w ...201323990290
cross-talk interactions of exogenous nitric oxide and sucrose modulates phenylpropanoid metabolism in yellow lupine embryo axes infected with fusarium oxysporum.the aim of the study was to examine cross-talk of exogenous nitric oxide (no) and sucrose in the mechanisms of synthesis and accumulation of isoflavonoids in embryo axes of lupinus luteus l. cv. juno. it was verified whether the interaction of these molecules can modulate the defense response of axes to infection and development of the pathogenic fungus fusarium oxysporum f. sp. lupini. sucrose alone strongly stimulated a high level of genistein glucoside in axes pretreated with exogenous nitric ...201323987816
construction of a new gfp vector and its use for fusaruim oxysporum transformation.in this study, the gfp fragment as a reporter gene had integrated into the form plasmid vector pbc-hygro which contains an expressive promoter of the fungus to facilitate the transformation of fusarium oxysporum. the resultant plasmid pbc-hygro-gfp was identified by digestion with enzymes. binary plasmids pbc-hygro-gfp were transformed into f. oxysporum by using the peg-cacl2 mediated transformation technique. results show that the recombinant plasmid pbc-hygro-gfp was constructed correctly. the ...201223961215
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...201123961146
characterization of a β-1,3-glucan recognition protein from the beet armyworm, spodoptera exigua (insecta: lepidoptera: noctuidae).the β-1,3-glucan recognition protein gene from spodoptera exigua (seβgrp) was cloned and characterized. the cdna of this gene is 1 644 nucleotides in length and the predicted polypeptide is 491 amino acids (aa) in length, with a calculated molecular mass of 54.8 kda. the first 22 aa encode a predicted secretion signal peptide. a blast search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of seβgrp revealed that this protein is most similar to the β-1,3-glucan recognit ...201323956146
use of a passive bioreactor to reduce water-borne plant pathogens, nitrate, and sulfate in greenhouse effluent.the goal of this study was to evaluate the use of passive bioreactors to reduce water-borne plant pathogens (pythium ultimum and fusarium oxysporum) and nutrient load (no(-) 3 and so(2-) 4) in greenhouse effluent. sterilized and unsterilized passive bioreactors filled with a reactive mixture of organic carbon material were used in three replicates. after a startup period of 2 (sterilized) or 5 (unsterilized) weeks, the bioreactor units received for 14 weeks a reconstituted commercial greenhouse ...201323947714
effects of electrokinetic operation mode on removal of polycyclic aromatic hydrocarbons (pahs), and the indigenous fungal community in pah-contaminated soil.electrokinetic remediation is an emerging physical remediation technology for the removal of heavy metals and organic chemicals from contaminated soil. we set up a soil chamber (24 × 12 × 8 cm) with two stainless steel electrodes (12 × 0.5 cm), and a constant voltage gradient of 1.0 v cm(-1) or 2.0 v cm(-1) was applied to study the effects of unidirectional and altered directional electric field operation modes on the moisture content and ph, the removal rate of pahs, and the abundance and diver ...201323947706
birth, death and horizontal transfer of the fumonisin biosynthetic gene cluster during the evolutionary diversification of fusarium.fumonisins are a family of carcinogenic secondary metabolites produced by members of the fusarium fujikuroi species complex (ffsc) and rare strains of fusarium oxysporum. in fusarium, fumonisin biosynthetic genes (fum) are clustered, and the cluster is uniform in gene organization. here, sequence analyses indicated that the cluster exists in five different genomic contexts, defining five cluster types. in fum gene genealogies, evolutionary relationships between fusaria with different cluster typ ...201323937442
rhizobacterium-mediated growth promotion and expression of stress enzymes in glycine max l. merrill against fusarium wilt upon challenge inoculation.wilt disease of soybean caused by a very common soil-borne fungus, fusarium oxysporum is one of the most destructive diseases of the crop. the aim of the present study was to characterize plant growth-promotion activities and induced resistance of a rhizobacterial strain for the soybean plant against f. oxysporum. rhizobacterium strain sj-5 exhibited plant growth-promotion characteristics and antagonistic activity against the test pathogen on dual plate assay. it was identified as a carnobacteri ...201423933805
contamination of bananas with beauvericin and fusaric acid produced by fusarium oxysporum f. sp. cubense.fusarium wilt, caused by the fungal pathogen fusarium oxysporum f. sp. cubense (foc), is one of the most destructive diseases of banana. toxins produced by foc have been proposed to play an important role during the pathogenic process. the objectives of this study were to investigate the contamination of banana with toxins produced by foc, and to elucidate their role in pathogenesis.201323922960
fusarium oxysporum infection assays in arabidopsis.increased susceptibility to fusarium oxysporum is one of the most conspicuous characteristics of the arabidopsis mutants lacking the heterotrimeric g protein β and γ1 subunits. the molecular mechanisms placing these g proteins in the plant innate immunity network are yet to be discovered. however, a method to test susceptibility to and disease progression of an important plant pathogen, such as f. oxysporum, is of central importance to many plant defense studies. the optimized protocol presented ...201323913036
[effects of nitrogen application rate on faba bean fusarium wilt and rhizospheric microbial metabolic functional diversity].a field plot experiment was conducted to study the effects of different nitrogen (n) application rates on the microbial functional diversity in faba bean rhizosphere and the relationships between the microbial functional diversity and the occurrence of faba bean fusarium wilt. four nitrogen application rates were installed, i. e. , n0(0 kg hm-2 , n1 (56. 25 kg hm-2) , n2(112. 5 kg hm-2), and n3 (168.75 kg hm-2), and biolog microbial analysis system was applied to study the damage of faba bean fu ...201323898671
biochemical markers assisted screening of fusarium wilt resistant musa paradisiaca (l.) cv. puttabale micropropagated clones.an efficient protocol was standardized for screening of panama wilt resistant musa paradisiaca cv. puttabale clones, an endemic cultivar of karnataka, india. the synergistic effect of 6-benzyleaminopurine (2 to 6 mg/l) and thidiazuron (0.1 to 0.5 mg/l) on ms medium provoked multiple shoot induction from the excised meristem. an average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/l 6-benzyleaminopurine and 0.3 mg/l thidiazuron concentrations. elongation of shoots observed on 5 mg/ ...201323898552
characterization of antifungal activity of the gh-46 subclass iii chitosanase from bacillus circulans mh-k1.we characterized the antifungal activity of the bacillus circulans subclass iii mh-k1 chitosanase (mh-k1 chitosanase), which is one of the most intensively studied glycoside hydrolases (ghs) that belong to gh family 46. mh-k1 chitosanase inhibited the growth of zygomycetes fungi, rhizopus and mucor, even at 10 pmol (0.3 μg)/ml culture probably via its fungistatic effect. the amino acid substitution e37q abolished the antifungal activity of mh-k1 chitosanase, but retained binding to chitotriose. ...201323892828
the antagonism activity of bacteria isolated from potato cultivated soil.soil-borne fungal and bacterial root pathogens can cause serious losses to agricultural crops. resistant plant varieties are not available for several soil-borne pathogens and chemical control is often insufficiently effective in soil. the enhancement of disease suppressive properties of soils will limit disease development, thus, being of great importance for sustainable agriculture as well as organic farming systems. the aim of this research is to find and identify suppressive soils in the sét ...201223878982
characterization of chlordecone-tolerant fungal populations isolated from long-term polluted tropical volcanic soil in the french west indies.the insecticide chlordecone is a contaminant found in most of the banana plantations in the french west indies. this study aims to search for fungal populations able to grow on it. an andosol heavily contaminated with chlordecone, perfused for 1 year in a soil-charcoal system, was used to conduct enrichment cultures. a total of 103 fungal strains able to grow on chlordecone-mineral salt medium were isolated, purified, and deposited in the miae collection (microorganismes d'intérêt agro-environne ...201423872892
slow sand filters effectively reduce phytophthora after a pathogen switch from fusarium and a simulated pump failure.slow sand filtration has been shown to effectively reduce phytophthora zoospores in irrigation water. this experiment tested the reduction of phytophthora colony forming units (cfus) by slow sand filtration systems after switching the pathogen contaminating plant leachate from fusarium to phytophthora and the resilience of the system to a short period without water, as might be caused by a pump failure. the slow sand filtration system greatly reduced phytophthora cfus and transmission after swit ...201323866129
plant defensin type 1 (pdf1): protein promiscuity and expression variation within the arabidopsis genus shed light on zinc tolerance acquisition in arabidopsis halleri.plant defensins are recognized for their antifungal properties. however, a few type 1 defensins (pdf1s) were identified for their cellular zinc (zn) tolerance properties after a study of the metal extremophile arabidopsis halleri. in order to investigate whether different paralogues would display specialized functions, the a. halleri pdf1 family was characterized at the functional and genomic levels. eleven pdf1s were isolated from a. halleri. their ability to provide zn tolerance in yeast cells ...201323865749
structural and functional diversity of rhizobacteria associated with rauwolfia spp. across the western ghat regions of karnataka, india.the present study carried out with denaturing gradient gel electrophoresis of dna extracted from rhizosphere soils of rauwolfia spp. collected from western ghat (wg) regions of karnataka indicated that pseudomonas sp. was prevalently found followed by methylobacterium sp., bacillus sp. and uncultured bacteria. a total of 200 rhizobacteria were isolated from 58 rhizosphere soil samples comprising of 15 different bacterial genera. the shannon weaver diversity index (h') and simpson's diversity ind ...201423864441
comparative mapping of raphanus sativus genome using brassica markers and quantitative trait loci analysis for the fusarium wilt resistance trait.fusarium wilt (fw), caused by the soil-borne fungal pathogen fusarium oxysporum is a serious disease in cruciferous plants, including the radish (raphanus sativus). to identify quantitative trait loci (qtl) or gene(s) conferring resistance to fw, we constructed a genetic map of r. sativus using an f2 mapping population derived by crossing the inbred lines '835' (susceptible) and 'b2' (resistant). a total of 220 markers distributed in 9 linkage groups (lgs) were mapped in the raphanus genome, cov ...201323864230
in vitro activity of miltefosine as a single agent and in combination with voriconazole or posaconazole against uncommon filamentous fungal pathogens.antifungal treatment of uncommon filamentous fungal infections is problematic. this study determined the in vitro susceptibility of miltefosine, as a single agent and in combination with posaconazole or voriconazole, against these pathogens.201323861311
proteomic identification of potential target proteins regulated by the scf(f) (bp1) -mediated proteolysis pathway in fusarium oxysporum.f-box proteins function in the recruitment of proteins for scf ubiquitination and proteasome degradation. here, we studied the role of fbp1, a nonessential f-box protein of the tomato pathogen fusarium oxysporum f. sp. lycopersici. the δfbp1 mutant showed a significant delay in the production of wilt symptoms on tomato plants and was impaired in invasive growth on cellophane membranes and on living plant tissue. to search for target proteins recruited by fbp1, a combination of sodium dodecylsulp ...201323855991
targeting iron acquisition blocks infection with the fungal pathogens aspergillus fumigatus and fusarium oxysporum.filamentous fungi are an important cause of pulmonary and systemic morbidity and mortality, and also cause corneal blindness and visual impairment worldwide. utilizing in vitro neutrophil killing assays and a model of fungal infection of the cornea, we demonstrated that dectin-1 dependent il-6 production regulates expression of iron chelators, heme and siderophore binding proteins and hepcidin in infected mice. in addition, we show that human neutrophils synthesize lipocalin-1, which sequesters ...201323853581
identification of immunity related genes to study the physalis peruviana--fusarium oxysporum pathosystem.the cape gooseberry (physalisperuviana l) is an andean exotic fruit with high nutritional value and appealing medicinal properties. however, its cultivation faces important phytosanitary problems mainly due to pathogens like fusarium oxysporum, cercosporaphysalidis and alternaria spp. here we used the cape gooseberry foliar transcriptome to search for proteins that encode conserved domains related to plant immunity including: nbs (nucleotide binding site), cc (coiled-coil), tir (toll/interleukin ...201323844210
fusaric acid induction of programmed cell death modulated through nitric oxide signalling in tobacco suspension cells.fusaric acid (fa) is a nonhost-selective toxin mainly produced by fusarium oxysporum, the causal agent of plant wilt diseases. we demonstrate that fa can induce programmed cell death (pcd) in tobacco suspension cells and the fa-induced pcd is modulated by nitric oxide (no) signalling. cells undergoing cell death induced by fa treatment exhibited typical characteristics of pcd including cytoplasmic shrinkage, chromatin condensation, dna fragmentation, membrane plasmolysis, and formation of small ...201323838885
optimization of media and temperature for enhanced antimicrobial production by bacteria associated with rhabditis sp.entomopathogenic nematodes, belonging to the family heterorhabditis and steinernematidae, are reported to be symbiotically associated with specific bacteria and the secondary metabolites produced by these bacteria possess antimicrobial activity. in this study, bacteria were isolated from nematodes belonging to the family rhabditidae, and the antimicrobial activity was tested against four bacteria viz. bacillus subtilis mtcc 2756, staphylococcus aureus mtcc 902, escherichia coli mtcc 2622, and ps ...201323825731
analysis of hairpin rna transgene-induced gene silencing in fusarium oxysporum.hairpin rna (hprna) transgenes can be effective at inducing rna silencing and have been exploited as a powerful tool for gene function analysis in many organisms. however, in fungi, expression of hairpin rna transcripts can induce post-transcriptional gene silencing, but in some species can also lead to transcriptional gene silencing, suggesting a more complex interplay of the two pathways at least in some fungi. because many fungal species are important pathogens, rna silencing is a powerful te ...201323819794
disseminated fusariosis secondary to neuroblastoma with fatal outcome.disseminated fusariosis is an uncommon clinical condition in immunocompromised patients. we report a fatal case of disseminated fusariosis secondary to neuroblastoma in a male patient, 15 years old, who underwent a bone marrow transplant. the patient was admitted to the pediatric intensive care unit (picu) of a public hospital in recife-pe, brazil, presenting bone marrow aplasia, severe leukopenia, and thrombocytopenia. after 15 days, the patient developed right knee effusion. synovial fluid and ...201323813153
identification and characterization of an anti-fungi fusarium oxysporum f. sp. cucumerium protease from the bacillus subtilis strain n7.a newly discovered alkaline antifungal protease named p6 from bacillus subtilis n7 was purified and partially characterized. b. subtilis n7 culture filtrates were purified by 30-60% (nh4)2so4 precipitation, anion-exchange chromatography and gel filtration chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) revealed a single band of 41.38 kda. peptide sequence of protease p6 was determined using a 4800 plus maldi tof/tof™ analyzer system. self-formed adaptor pcr ( ...201323812816
diversity in receptor-like kinase genes is a major determinant of quantitative resistance to fusarium oxysporum f.sp. matthioli.resistance to wilt fungus fusarium oxysporum f.sp. matthioli (fom) is a polygenic trait in arabidopsis thaliana. rfo3 is one of six quantitative trait loci accounting for the complete resistance of accession columbia-0 (col-0) and susceptibility of accession taynuilt-0 (ty-0). we find that col-0 and ty-0 alleles of rfo3 are representative of two common variants in wild arabidopsis accessions, that resistance and susceptibility to fom are ancestral features of the two variants and that resistance ...201323790083
insights into fungal communities in composts revealed by 454-pyrosequencing: implications for human health and safety.fungal community composition in composts of lignocellulosic wastes was assessed via 454-pyrosequencing of its1 libraries derived from the three major composting phases. ascomycota represented most (93%) of the 27,987 fungal sequences. a total of 102 genera, 120 species, and 222 operational taxonomic units (otus; >97% similarity) were identified. thirty genera predominated (ca. 94% of the sequences), and at the species level, sequences matching chaetomium funicola and fusarium oxysporum were the ...201323785368
antibacterial activity of extracellular compounds produced by a pseudomonas strain against methicillin-resistant staphylococcus aureus (mrsa) strains.the emergence of multidrug-resistant bacteria is a world health problem. staphylococcus aureus, including methicillin-resistant s. aureus (mrsa) strains, is one of the most important human pathogens associated with hospital and community-acquired infections. the aim of this work was to evaluate the antibacterial activity of a pseudomonas aeruginosa-derived compound against mrsa strains.201323773484
antifungal, insecticidal, and plant growth promoting potential of streptomyces hydrogenans dh16.in the present study, an actinobacterium strain, possessing antagonistic activity against different fungal phytopathogens viz. colletotrichum acutatum, cladosporium herbarum, alternaria brassicicola, exserohilum sp., alternaria mali, colletotrichum gleospoiroides, alternaria alternata, cercospora sp., fusarium oxysporum f.sp. dianthi and fusarium moniliformae, was isolated from soil and identified as streptomyces hydrogenans dh16. application of culture supernatant (5%)/cells (10(7)  cfu ml(-1) ...201423765423
streptomyces rochei acta1551, an indigenous greek isolate studied as a potential biocontrol agent against fusarium oxysporum f.sp. lycopersici.many studies have shown that several greek ecosystems inhabit very interesting bacteria with biotechnological properties. therefore streptomyces isolates from diverse greek habitats were selected for their antifungal activity against the common phytopathogenic fungus fusarium oxysporum. the isolate encoded acta1551, member of streptomyces genus, could strongly suppress the fungal growth when examined in antagonistic bioassays in vitro. the isolate was found phylogenetically relative to streptomy ...201323762841
modified primers for the identification of nonpathogenic fusarium oxysporum isolates that have biological control potential against fusarium wilt of cucumber in taiwan.previous investigations demonstrated that fusarium oxysporum (fo), which is not pathogenic to cucumbers, could serve as a biological control agent for managing fusarium wilt of cucumber caused by fo f. sp. cucumerinum (foc) in taiwan. however, thus far it has not been possible to separate the populations of pathogenic fo from the nonpathogenic isolates that have biological control potential through their morphological characteristics. although these two populations can be distinguished from one ...201323762289
fusariotoxins in asparagus - their biosynthesis and migration.asparagus is often infected by fungi of the fusarium genus, a causal agent of crown and root rot, which decreases the quantity and quality of spears. fusarium oxysporum and fusarium proliferatum are the most severe asparagus pathogens, well known as mycotoxin producers, mainly fumonisins and moniliformin. the present study was undertaken to estimate fumonisin b1, moniliformin and ergosterol concentrations in asparagus tissue. moreover, the possibility of toxin transport to the edible asparagus p ...201323756238
preparative thin-layer chromatographic separation followed by identification of antifungal compound in cassia laevigata by rp-hplc and gc-ms.several species of the genus cassia are known for their antioxidant, antimicrobial and antidiabetic activities, but some of the lesser-known cassia species, e.g. c. renigera, c. biflora and c. laevigata have not been studied for their biological activities.201423749313
an iron 13s-lipoxygenase with an α-linolenic acid specific hydroperoxidase activity from fusarium oxysporum.jasmonates constitute a family of lipid-derived signaling molecules that are abundant in higher plants. the biosynthetic pathway leading to plant jasmonates is initiated by 13-lipoxygenase-catalyzed oxygenation of α-linolenic acid into its 13-hydroperoxide derivative. a number of plant pathogenic fungi (e.g. fusarium oxysporum) are also capable of producing jasmonates, however, by a yet unknown biosynthetic pathway. in a search for lipoxygenase in f. oxysporum, a reverse genetic approach was use ...201323741422
transformation of pwwo in rhizobium leguminosarum dpt to engineer toluene degrading ability for rhizoremediation.rhizoremediation of organic xenobiotics is based on interactions between plants and their associated micro-organisms. the present work was designed to engineer a bacterial system having toluene degradation ability along with plant growth promoting characteristics for effective rhizoremediation. pwwo harboring the genes responsible for toluene breakdown was isolated from pseudomonas putida mtcc 979 and successfully transformed in rhizobium dpt. this resulted in a bacterial strain (dpt(t)) which h ...201123729882
phenazine antibiotic production and antifungal activity are regulated by multiple quorum-sensing systems in pseudomonas chlororaphis subsp. aurantiaca stfrb508.a number of gram-negative bacteria have a quorum-sensing system and produce the n-acylhomoserine lactone (ahl) as a signal molecule. pseudomonas chlororaphis subsp. aurantiaca stfrb508 produces one of the phenazine derivatives, phenazine-1-carboxylic acid (pca). whole-genome sequencing of stfrb508 revealed the presence of two sets of ahl-synthase and ahl-receptor gene, phzir and aurir. the mutation of phzi drastically decreased pca production, but the mutation of auri did not affect pca producti ...201323727350
antifungal activity and cytotoxicity of isolated compounds from leaves of breonadia salicina.breonadia salicina is used traditionally to treat wounds, ulcers, fevers, headaches, and fungal infections. the aim of this study was to investigate the antifungal activity of the plant extract and compounds isolated there from.201323727185
[fusariosis diagnosed in the laboratory of an uh in tunisia: epidemiological, clinical and mycological study].the genus fusarium, initially known for its important agro-economic impact, is more and more often implicated in human pathology. in fact, multiples allergic, toxic and infectious manifestations are more reported in immunocompetent and immunocompromised hosts. the objective of our study was to analyse the epidemiological, mycological and clinical features of fusariosis reported in our chu. eighty-seven cases of fusarium infections were collected: 34 cases of onychomycosis (39%), 26 cases of inte ...201323725904
lipolytic system of the tomato pathogen fusarium oxysporum f. sp. lycopersici.the lipolytic profile of fusarium oxysporum f. sp lycopersici was studied by in silico search and biochemical enzyme activity analyses. twenty-five structural secreted lipases were predicted based on the conserved pentapeptide gly-x-ser-x-gly-, characteristic of fungal lipases, and secretion signal sequences. moreover, a predicted lipase regulatory gene was identified in addition to the previously characterized ctf1. the transcription profile of thirteen lipase genes during tomato plant coloniza ...201323718123
arabidopsis thaliana resistance to fusarium oxysporum 2 implicates tyrosine-sulfated peptide signaling in susceptibility and resistance to root infection.in the plant arabidopsis thaliana, multiple quantitative trait loci (qtls), including rfo2, account for the strong resistance of accession columbia-0 (col-0) and relative susceptibility of taynuilt-0 (ty-0) to the vascular wilt fungus fusarium oxysporum forma specialis matthioli. we find that rfo2 corresponds to diversity in receptor-like protein (rlp) genes. in col-0, there is a tandem pair of rlp genes: rfo2/at1g17250 confers resistance while rlp2 does not. in ty-0, the highly diverged rfo2 lo ...201323717215
fungal endophytes from three cultivars of panax ginseng meyer cultivated in korea.in order to investigate the diversity of endophytes, fungal endophytes in panax ginseng meyer cultivated in korea were isolated and identified using internal transcribed spacer (its) sequences of ribosomal dna. three cultivars of 3-year-old ginseng roots (chunpoong, yunpoong, and gumpoong) were used to isolate fungal endophytes. surface sterilized ginseng roots were placed on potato dextrose agar plates supplemented with ampicilin and streptomycin to inhibit bacterial growth. overall, 38 fungal ...201223717111
hyperkeratotic warty skin lesion of foot caused by fusarium oxysporum.fusarium species are common soil-inhabiting organisms and plant pathogens. human infections are usually precipitated by local or systemic predisposing factors, and disseminated infection is associated with impaired immune responses. skin infections caused by fusarium spp. include keratitis, onychomycosis, mycetoma, painful discrete erythematous nodules. hyperkeratotic skin lesions caused by fusarium spp. are, however, rarely reported. we report a case of hyperkeratotic verrucous warty skin lesio ...201323716829
epi-trichosetin, a new undecaprenyl pyrophosphate synthase inhibitor, produced by fusarium oxysporum fki-4553.a new compound, designated epi-trichosetin (1), was isolated along with the known compound trichosetin (2) from the culture broth of fusarium oxysporum fki-4553 by solvent extraction, silica gel column chromatography and reversed-phase hplc. the structure of 1 was elucidated by comparing various spectral data with those of 2, revealing that 1 was a stereoisomer of 2. compounds 1 and 2 inhibited the undecaprenyl pyrophosphate synthase activity of staphylococcus aureus with ic50 values of 83 and 3 ...201323715038
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