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toxicological approaches to the metabolites of fusaria. xi. trichothecenes and zearalenone from fusarium species isolated from river sediments.fifty isolates of fusarium species from river sediments were examined on their producibility of mycotoxins such as trichothecenes and zearalenone. the most frequent fungal isolate was fusarium oxysporum, followed by f. solani and f. lateritium. when cultured on czapek-dox medium supplemented with peptone, more than half of these strains were suspected to produce trichothecene mycotoxins through toxicity to mice and reticulocyte assay method. one of toxic isolates, f. lateritium 5036, was proved ...1977916296
the occurrence of 12, 13-epoxytrichothecenes in seeds of safflower infected with fusarium oxysporum f. sp. carthami.from the seeds of safflower (carthamus tinctorius linn.), infected with fusarium oxysporum f. sp. carthami, 3 toxic compounds have been isolated in quantities sufficient to cause mycotoxicosis on prolonged ingestion. 2 of these have been identified as diacetoxyscirpenol and t-2 toxin, while the third one has also been partially characterized as a 12, 13-epoxytrichothecene. additionally, the incidence of secondary fusarial infection of healthy seeds due to contamination with the infected ones has ...1977862770
isolation of ethyl p-methoxycinnamate, the major antifungal principle of curcumba zedoaria.an antifungal principle of the dried rhizomes of curcuma zedoaria was extracted with hot ethanol. by successive chromatography on neutral alumina and silica gel, three antibiotic compounds a, b, and c, all active against trichophyton rubrum, aspergillus niger and saccharomyces cerevisiae, were obtained in chemically pure form. by uv, ir, pmr and ms analysis, the structure of the most abundant one of these compounds (c, 69.8%; h, 6,8%; and 0.23.4%) was assigned as ethyl p-methoxycinnamate (epmc). ...1976785141
the influence of water-logging stage upon the paddy soil mycoflora in the ukraine.the soil fungal flora of paddy soils from the southern districts of the ukraine was investigated. from 287 soil samples 170 species were isolated. they belonged to the following genera: oomycetes 1.2%, zygomycetes 8.8%, ascomycetes 5.8%, deuteromycetes 84.2%. certain fungi were found to be dominant forms, among them mortierella alpina peyron., penicillium funiculosum thom, p. rugulosum thom, p. chrysogenum thom, acremonium strictum w. gams, acr. breve w. gams, alternaria alternata (fr.) riessler ...1978726714
lipid metabolism in fusarium oxysporum. 1978723228
effect of different cultural conditions on the chemical composition of lipids of fusarium oxysporum. 1978723227
effect of c.c.c. [2-(chloroethyl)] trimethyl ammonium chloride on growth and sporulation in fusarium oxysporum f. udum (butl.) sn. et h.in fusarium oxysporum f. udum sporulation of macroconidia was completely inhibited due to the influence of c.c.c. [2-(chloroethyl)] trimethyl ammonium chloride). microconidial proliferation, on the other hand, was adversely affected and with increase in concentration of this compound a parallel and proportional decrease in the amount of microconidia was found. size of microconidia, too, showed a concomitant decline. although the size of the chlamydospores was also reduced, a tremendous increase ...1978664938
effect of different concentrations of dl-isoleucine, dl-valine, and dl-alanine on growth and sporulation in fusarium oxysporum f. udum (butl.) sn. et h.d1-alanine and dl-valine, when added as an extra nitrogen for fortifying the already present inorganic nitrogen source, actually acted as growth retardant for f. oxysporum f. udum (butl.) sn. et h. sporulation of microconidia was indifferently affected by these two amino acids. di-valine stimulated microconidial formation in young cultures only. in both young and old cultures the lowest concentration of dl-valine depressed macronidial sporulation. in old cultures the lowest concentration of vali ...1977610233
utilization of kerosene by a hydrocarbon-assimilating strain of fusarium oxisporum.fusarium oxysporum ifo 6384 was grown in three different media, one containing glucose and two containing kerosene as carbon source. one of the kerosene media contained also tween 60. the harvested dry mats were weighed at various stages of growth. substitution of glucose with kerosene led to a sharp drop in the production of fungal mycelia. analysis of the mat samples representing maximum yields indicated that the mycelia grown in the kerosene media were richer in free amino acid and poorer in ...1977602572
effect of leaf extract of garlic on fusarium oxysporum f. sp. ciceri, sclerotinia sclerotiorum and on gram seeds. 1979574190
comparative studies with regard to the influence of carbon and nitrogen ratio on sporulation in fusarium oxysporum and fusarium moniliforme v. subglutinans.carbon/nitrogen ratio as a factor for sporulation, expressed in terms of magnitude of population variation of macroconidia and microconidia in the cultures of eusarium oxysporum schlecht ex. fr., fusarium moniliforme v. subglutinans wr. and rg., and of chlamydospores (only in fusarium oxysporum) was investigated. it has been found that the amount of carbon source shapes the course of macro- and micro. conidial production in a linear fashion, being enhanced parallel to the increase in its amount- ...1979543920
changes in metabolic activities of fusarium oxysporum f. fabae and rhizoctonia solani in response to dithan a-40 fungicide.the effect of different concentrations of dithan a-40 fungicide on the metabolic activities of the wilt fungus fusarium oxysporum f. fabae and the root rot agent rhizoctonia solani was studied. all toxicant concentrations reduced energy generation, total phosphorus and nitrogen content of both fungi. in addition, the toxicant caused a shift in free amino acids pool. as a result of these changes, the mycelium dry weight of both fungi was greatly reduced. r. solani was more sensitive to the toxic ...1979543918
vitamin requirements of fusarium oxysporum f. sp. vasinfectum.the effect of eight water-soluble vitamins on germination, germ-tube extension, growth, and sporulation of fusarium oxysporum schl. f.sp. vasinfectum (atk.) snyder and hansen, was studied. each vitamin was utilized in eight different concentrations. the fungus responded favourably to all of the utilized vitamins in almost all the concentrations where germination, growth, and sporulation were substantially greater than the controls. among the vitamins used, the fungus appeared to be highly sensit ...1979543346
isolation of metarrhizium anisopliae, beauveria tenella and fusarium oxysporum (deuteromycetes) and their pathogenicity to culex fatigans and anopheles stephensi. 1979535971
studies on the extracellular cellulase (cx) from fusarium oxysporum. 1979535933
the fine structure of mature and germinating chlamydospores of fusarium oxysporum.a number of features not described previously has been revealed in electron-microscope studies of mature chlamydospores of fusarium oxysporum. on the maturation of one isolate, many spores formed a thick matrix-like layer containing electron-dense particles on the exterior surface of the spore wall. in thin sections of mature chlamydospores of the same isolate, cisternae of endoplasmic reticulum surrounding, and in close apposition to, the limiting boundary of the lipid bodies were revealed. the ...1979476555
different manifestations of the pathogenity of some strains of fusarium oxysporum f. sp. pisi.different cultivation and morphology characteristics were found in 10 monospore isolates of fusarium oxysporum f. sp. pisi, obtained from yellowing and wilting plants of pea (pisum sativum l.). the isolates of the fungus were obtained from distant geographical regions of czechoslovakia and from various cultivars and hybrids of pea. after inoculation of roots, followed by constant conditions of incubation of the meteor and jupiter cultivars having their origin at the plant-breeding station at luz ...1979473967
the effect of temperature and age of strains of fusarium oxysporum on its enzymatic activity.the activities of pectin-methyl-esterase, polygalacturonase, cellulase, amylase, saccharase, and protease of strains of fusarium oxysporum (schlecht.) f.sp. pisi (linford) were studied. the selected strains showed different symptoms and different degrees of pathogenicity on the host plant. the measurements were performed during the growth of strains at constant temperatures and, in another experiment, on the day at different temperatures at which the individual strains were grown. activities wer ...1979473966
developmental regulation of invertase isozymes in fusarium oxysporum.fusarium invertase has two isozymes; one is developmentally regulated, whereas the other exists throughout the developmental stages of fusarium.1979457615
production of 3-acetoxyscirpene-4,15-diol from anguidine (4,15-diacetoxyscirpene-3-ol) by fusarium oxysporum f.sp. vasinfectum.growing cells of fusarium oxysporum f.sp. vasinfectum (atcc 7808) formed 3-acetoxyscirpene-4,15-diol from anguidine (4,15-diacetoxyscirpene-3-ol) by way of the intermediates triacetoxyscirpene, 3,4-diacetoxyscirpene-15-ol and 3,15-diacetoxyscirpene-4-ol. the new 3-acetoxy analog was found to be less active than anguidine and the other monoacetoxy derivatives when tested against a series of fungal strains and against hela cells in vitro.1979453837
populations of the spinach wilt pathogen, fusarium oxysporum f. sp. spinaciae, in the root tissues, rhizosphere, and soil in the field.populations of fusarium oxysporum f. sp. spinaciae in root tissues and rhizosphere soil of diseased spinach plants were higher than in the root tissues and rhizosphere soil of healthy plants. populations in soil rhizosphere were higher than in nonrhizosphere soil. the fungus populations were very low in the root tissues of the nonsusceptible strawberry, broccoli, chinese cabbage, and mustard grown in the infested field. the populations were low at the beginning of the season, increased, and rema ...1979436018
opportunistic fungal infection by fusarium oxysporum in a renal transplant patient.a white female with chronic glomerular nephritis received a renal transplant in 1971. after 1 year, livido reticularis (lr) developed and in 1976 erythematous, painful nodules formed on the lr and ulcerated. the patient also suffered diffuse calcification of the major blood vessels and small arterioles of the extremities with progressive necrosis and gangrene of the fingers. hyperparathyroidism was evident. the necrotic ulcers yielded candida albicans and fusarium oxysporum; both organisms were ...1979394364
fusarium oxysporum endophthalmitis. 1979313780
fusarium oxysporum endophthalmitis.a 27-year-old man sustained a thorn injury to his left eye and a culture-proven fusarium oxysporum endophthalmitis developed. this was successfully treated with a vitrectomy, intravitreal and intravenous amphotericin b, and oral flucytosine. the patient maintains 20/20 vision at this time in his left eye. we recommend combined therapy for this filamentous fungal infection.1979310293
properties of spin labelled membranes of fusarium oxysporum f. sp. lycopersici.growth temperature-induced compositional changes in membranes of fusarium oxysporum provided a test system for study of the relationship between physical properties and composition. growth at 15 degrees c was characterized by a decrease in phospholipid content relative to sterol content, a shift in phospholipid composition from phosphatidylcholine to phosphatidylethanolamine and a marked enhancement in the amount of polyunsaturated fatty acids in the phospholipid and triglyceride classes. uptake ...1976182261
chemical mutagenesis of fusarium oxysporum f. sp. lycopersici: non-selected changes in pathogenicity of auxotrophic mutants.single and multiple auxotrophic mutants of the fusarium oxysporum f. sp.lycopersici strains which cause fusarium crown-rot and fusarium wilt of tomato were obtained by chemical mutagenesis with nitrous acid, nitrosoguandidine and ethylmethaneulphonate. the mutagenesis and selection procedures, adapted for use with a plant pathogenic fungus, are described. changes in pathogenicity were observed when the auxotrophs were compared with the wild type but no correlation was observed between changes in ...1975167104
effect of three nematicides on the growth of some phytopathogenic bacteria and fungi.the effect of three nematicides, aldicarb, fensulfothion, and phenamiphos at four concentrations (1, 5, 25, and 125 ppm) was tested on the growth of five bacteria, agrobacterium tumefaciens, corynebacterium fascians, erwinia carotovora, pseudomonas solanacearum, and streptomyces scabies and four fungi, fusarium oxysporum f. sp. vasinfectum, fusarium solani, rhizoctonia solani, and sclerotium bataticola. of the bacteria, p. solanacearum was most affected by the chemicals at all concentrations, wh ...1977143857
crystallization and positional specificity of hydroperoxidation of fusarium lipoxygenase.a lipoxygenase obtained from the fungus fusarium oxysporum was purified and crystallized. using the purified enzyme, the positional specificity of linoleate peroxidation was studied. linoleate hydroperoxides were converted into the corresponding trimethylsilyl derivative by reduction, catalytic hydrogenation and treatment with hexamethyldisilazane/trimethylchlorosilane/pyridine and then analyzed by combined gas-liquid chromatography-mass spectrometry. fusarium lipoxygenase was found to produce 9 ...1978100142
growth temperature-dependent stearoyl coenzyme a desaturase activity of fusarium oxysporum microsomes.the characteristics of the microsomal stearoyl coa desaturase (ec 1.14.99.5) of vegetative fusarium oxysporum cells grown at different temperatures were studied. the enzyme had an unusual preference for nadph (km = 38 micrometers) over nadh (km = 89 micrometers) as electron donor, and a relatively high optimum ph of 8.3. enzyme activity was highest in microsomes from cells grown at 37 degrees c and lowest in cells grown at 15 degrees c. this result correlated well with the observed changes in ol ...197835271
circular dichroism of fusarium lipoxygenase from fusarium oxysporum. 197833673
hydroxymethylglutaryl-coenzyme a reductase. purification and properties of the enzyme from fusarium oxysporum.the hydroxymethylglutaryl-coenzyme a reductase (mevalonate:nadp+ oxidoreductase, ec 1.1.1.34) system in fusarium oxysporum, a soil inhabiting plant pathogen, has been examined. two forms of the enzyme catalyzing the conversion of hydroxymethylglutaryl-coenzyme a were obtained in the supernatant after precipitation at 75% (nh4)2so4 saturation of the soluble culture extract which was previously separated from cell wall, mitochondria and microsomes. the two forms of the enzyme were separated electr ...197826407
purification and properties of nitroalkane oxidase from fusarium oxysporum.a nitroalkane-oxidizing enzyme, which was inducibly formed by addition of nitroethane to the medium was purified to homogeneity from an extract of fusarium oxysporum (ifo 5942) with an overall yield of about 20%. the enzyme catalyzed the oxidative denitrification of 1-nitropropane as follows: ch2(no2)ch2ch3 + o2 + h2o leads to ohcch2ch3 + hno2 + h2o2. in addition to 1-nitropropane, 3-nitro-2-pentanol, 2-nitropropane, and nitrocyclohexane are good substrates; the enzyme is designated "nitroalkane ...197822538
regulation of pyruvate kinases from fusarium oxysporum.two types of pyruvate kinases were found in fusarium oxysporum. one type (inducible) was present mainly during the early stages of growth on glucose or sucrose and displayed michaelis-menten kinetics with respect to phosphoenolpyruvate and adenosine diphosphate. the major type (constitutive) was present under all conditions of growth and displayed in the absence of potassium ions, a sigmoidal substrate saturation curve when phosphoenolpyruvate was used as the variable substrate. in the presence ...197721735
studies on sterol-ester hydrolase from fusarium oxysporum. i. partial purification and properties.1. a search for a long chain fatty acyl sterol-ester hydrolase in microorganisms led to the isolation from soil of five strains belonging to fusarium sp. which produced strong activity in the culture medium. 2. the cholesterol esterase from fusarium oxysporum igh-2 was purified about 270-fold by means of cacl2 precipitation and sephadex g-75 column chromatography. 3. the cholesterol esterase was activated by adekatol and triton x-100. it was inhibited by lecithin and lysolecithin, and completely ...197719426
effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph.fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ...197718085
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