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biocontrol potential of halotolerant bacterial chitinase from high yielding novel bacillus pumilus mcb-7 autochthonous to mangrove ecosystem.the multifaceted role of chitinase in medicine, agriculture, environmental remediation and various other industries greatly demands the isolation of high yielding chitinase producing microorganisms with improved properties. the current study aimed to investigate the isolation, characterization and biocontrol prospective of chitinase producing bacterial strains autochthonous to the extreme conditions of mangrove ecosystems. among the 51 bacterial isolates screened, bacillus pumilus mcb-7 with hig ...201728364802
antifungal activity, yield, and composition of ocimum gratissimum essential oil.ocimum gratissimum l. or clove basil, belongs to the lamiaceae family, has various desirable uses and applications. beyond its aromatic, seasoning, and medicinal applications, this plant also has antimicrobial activity. this study was aimed at assessing the antifungal activity, yield, and composition of the essential oil (eo) of o. gratissimum. the species was cultivated in garden beds with dystrophic red latosol soil type containing high organic-matter content. the eo was obtained by hydrodisti ...201728362991
members of gammaproteobacteria as indicator species of healthy banana plants on fusarium wilt-infested fields in central america.culminating in the 1950's, bananas, the world's most extensive perennial monoculture, suffered one of the most devastating disease epidemics in history. in latin america and the caribbean, fusarium wilt (fw) caused by the soil-borne fungus fusarium oxysporum f. sp. cubense (foc), forced the abandonment of the gros michel-based export banana industry. comparative microbiome analyses performed between healthy and diseased gros michel plants on fw-infested farms in nicaragua and costa rica revealed ...201728345666
concurrent methylation and demethylation of arsenic by fungi and their differential expression in the protoplasm proteome.microbial methylation and demethylation are central to arsenic's (as) biogeochemical cycling. here, the transformations of monomethylarsonic acid (mma(v)) (50 mg l(-1)) for 15 days in cells of as-methylating fungi, fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1, were evaluated, and trace concentrations of as(iii) and as(v) were observed in fungal cell extracts. trace amounts of dma(v) were also detected in mma(v) and p. janthinellum sm-12f4 incuba ...201728336093
biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from bacillus pumilus jubch08 displaying antagonism against phytopathogenic fusarium oxysporum.the present investigation highlights the process parameters influencing the submerged fermentation of chitinase by bacillus pumilus jubch08, purification and characterization of the enzyme and determination of antagonistic activity of the bacterium against fusarium oxysporum. medium supplemented with 0.5 % chitin and peptone, at initial ph 8.0, when incubated at 35 °c for 72 h favored highest chitinase production. the enzyme was purified 25.1-fold to homogeneity. the chitinase was found to be th ...201628330157
comparison of dna microarray, loop-mediated isothermal amplification (lamp) and real-time pcr with dna sequencing for identification of fusarium spp. obtained from patients with hematologic malignancies.the performance of three molecular biology techniques, i.e., dna microarray, loop-mediated isothermal amplification (lamp), and real-time pcr were compared with dna sequencing for properly identification of 20 isolates of fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. dna microarray, lamp and real-time pcr identified 16 (80%) out of 20 samples as fusarium solani species complex (fssc) and four (20%) as fusarium spp. t ...201728324245
effector gene suites in some soil isolates of fusarium oxysporum are not sufficient predictors of vascular wilt in tomato.seventy-four f. oxysporum soil isolates were assayed for known effector genes present in an f. oxysporum f.sp. lycopersici race 3 tomato wilt strain (fol mn-25) obtained from the same fields in manatee county, florida. based on the presence or absence of these genes, four haplotypes were defined, two of which represented 96% of the surveyed isolates. these two most common effector haplotypes contained either all or none of the assayed race 3 effector genes. we hypothesized that soil isolates wit ...201728323535
trans-cinnamic acid and xenorhabdus szentirmaii metabolites synergize the potency of some commercial fungicides.development of novel approaches for the control of fungal phytopathogens is desirable. in this study we hypothesized that the combination of commercial fungicides with certain enhancing agents could result in synergistic levels of control. prior research has indicated that trans-cinnamic-acid (tca), a metabolite of the bacteria photorhabdus luminescens and metabolites of xenorhabdus szentirmaii are particularly toxic to various phytpathogenic fungi when compared to metabolites of other xenorhabd ...201728322849
plant growth-promoting endophytic bacteria versus pathogenic infections: an example of bacillus amyloliquefaciens rwl-1 and fusarium oxysporum f. sp. lycopersici in tomato.fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic bacillus amyloliquefaciens rwl-1 against pathogenic fusarium oxysporum f. sp. lycopersici. the results revealed significant suppressio ...201728321368
mineralization of cellulose in the presence of chitin and assemblages of microflora and fauna in soil.nitrogen content is an important factor controlling decomposition of resistant substrates. we examined the decomposition of purified cellulose in the presence of a structurally similar, resistant organic n compound, chitin. carbon-14-labelled cellulose was added to sterile ari-dried sandy loam soil in flasks, half of which were also amended with purified chitin. grassland soil organisms-a fungus (fusarium oxysporum) or a bacterium (flavobacterium sp.) with or without their respective nematode gr ...198328310541
rhizosphere and root-infecting fungi and the design of ecological field experiments.as part of a wider study into the role of soil fungi in the ecology of the winter annual grass, vulpia ciliata ssp. ambigua (le gall) stace & auquier, we applied the fungicides benomyl and prochloraz to three natural populations of the grass growing in east anglia, united kingdom. the rhizosphere and rootinfecting fungi associated with the three populations were analysed each month between february and may 1992 when plants set seed. there were marked differences between the fungal floras associa ...199528306878
draft genome sequence of rhizobium sp. strain tbd182, an antagonist of the plant-pathogenic fungus fusarium oxysporum, isolated from a novel hydroponics system using organic fertilizer.rhizobium sp. strain tbd182, isolated from a novel hydroponics system, is an antagonistic bacterium that inhibits the mycelial growth of fusarium oxysporum but does not eliminate the pathogen. we report the draft genome sequence of tbd182, which may contribute to elucidation of the molecular mechanisms of its fungistatic activity.201728302768
nitrate increased cucumber tolerance to fusarium wilt by regulating fungal toxin production and distribution.cucumber fusarium wilt, induced by fusarium oxysporum f. sp. cucumerinum (foc), causes severe losses in cucumber yield and quality. nitrogen (n), as the most important mineral nutrient for plants, plays a critical role in plant-pathogen interactions. hydroponic assays were conducted to investigate the effects of different n forms (nh₄⁺ vs. no₃(‒)) and supply levels (low, 1 mm; high, 5 mm) on cucumber fusarium wilt. the no₃(‒)-fed cucumber plants were more tolerant to fusarium wilt compared with ...201728287458
medium ph in submerged cultivation modulates differences in the intracellular protein profile of fusarium oxysporum.fusarium oxysporum is a filamentous fungus that damages a wide range of plants and thus causes severe crop losses. in fungal pathogens, the genes and proteins involved in virulence are known to be controlled by environmental ph. here, we report the influence of culture-medium ph (5, 6, 7, and 8) on the production of degradative enzymes involved in the pathogenesis of f. oxysporum urm 7401 and on the 2d-electrophoresis profile of intracellular proteins in this fungus. f. oxysporum urm 7401 was gr ...201728281888
disseminated phaeohyphomycosis in a dog.phaeohyphomycosis is a rare but emerging disease caused by dematiaceous fungi. here we describe the case of an immunosuppressed dog with disseminated phaeohyphomycosis secondary to bipolaris spicifera infection. regionally extensive infiltration of the paw pads, skin, myocardium, liver, renal interstitium and diaphragm was identified on histopathology. candida glabrata and fusarium oxysporum were also cultured from multiple sites post-mortem. the dog was treated with fluconazole, itraconazole, t ...201728275534
diversity, phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with monarda citriodora l.present study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of the monarda citriodora cerv. ex lag. (lamiaceae/labiatae). anticancer and antimicrobial potential of isolated endophytes have also been investigated.201728264654
the plasma membrane-localized sucrose transporter ibsweet10 contributes to the resistance of sweet potato to fusarium oxysporum.sweet (sugars will eventually be exported transporter) proteins, a novel family of sugar transporters, mediate the diffusion of sugars across cell membranes and acts as key players in sucrose phloem loading. manipulation of sweet genes in plants leads to various effects on resistance to biotic and abiotic stresses due to disruption of sugar efflux and changes in sugar distribution. in this study, a member of the sweet gene family, ibsweet10, was cloned from the sweet potato line nd98. mrna expre ...201728261250
response of atnpr1-expressing cotton plants to fusarium oxysporum f. sp. vasinfectum isolates.in our earlier investigation, we had demonstrated that transgenic cotton plants expressing atnpr1 showed significant tolerance to fusarium oxysporum f. sp. vasinfectum, isolate 11 (fov11) and several other pathogens. the current study was designed to further characterize the nature of the protection provided by atnpr1 expression and its limitations. green fluorescent protein-expressing fov11 was generated and used to study the progression of the disease within the plant. the results show that th ...201728250590
identification of biomarkers for resistance to fusarium oxysporum f. sp. cubense infection and in silico studies in musa paradisiaca cultivar puttabale through proteomic approach.panama wilt caused by fusarium oxysporum f. sp. cubense (foc) is one of the major disease constraints of banana production. previously, we reported the disease resistance musa paradisiaca cv. puttabale clones developed from ethylmethanesulfonate and foc culture filtrate against foc inoculation. here, the same resistant clones and susceptible clones were used for the study of protein accumulation against foc inoculation by two-dimensional gel electrophoresis (2-de), their expression pattern and a ...201628248219
<i>fusarium</i> species isolated from <i>pennisetum clandestinum</i> collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201428235286
antimicrobial activity of medicinal plants correlates with the proportion of antagonistic endophytes.medicinal plants are known to harbor potential endophytic microbes, due to their bioactive compounds. in a first study of ongoing research, endophytic bacteria were isolated from two medicinal plants, hypericum perforatum and ziziphora capitata with contrasting antimicrobial activities from the chatkal biosphere reserve of uzbekistan, and their plant-specific traits involved in biocontrol and plant growth promotion were evaluated. plant extracts of h. perforatum exhibited a remarkable activity a ...201728232827
suppression of fusarium wilt of cucumber by ammonia gas fumigation via reduction of fusarium population in the field.cucumber plants subjected to consecutive monoculture for 9 years were found to suffer from severe fusarium wilt disease, caused by the soil-borne fungus fusarium oxysporum f. sp. cucumerinum j. h. owen. in the present study, greenhouse experiments were performed to evaluate the influence of ammonia gas fumigation on fusarium wilt suppression, fungal abundance and fungal community composition. results showed that ammonia gas fumigation remarkably reduced disease incidence from 80% to 27%, resulti ...201728230182
multiple garlic (allium sativum l.) micrornas regulate the immunity against the basal rot fungus fusarium oxysporum f. sp. cepae.the basal plate rot fungus, fusarium oxysporum f. sp. cepae (foc), is the most devastating pathogen posing a serious threat to garlic (allium sativum l.) production worldwide. micrornas (mirnas) are key modulators of gene expression related to development and defense responses in eukaryotes. however, the mirna species associated with garlic immunity against foc are yet to be explored. in the present study, a small rna library developed from foc infected resistant garlic line was sequenced to ide ...201728224922
effectiveness of neutral electrolyzed water on incidence of fungal rot on tomato fruits ( solanum lycopersicum l.).we assessed the effect of neutral electrolyzed water (new) on the incidence of rot on tomato ( solanum lycopersicum l.) fruits inoculated with fusarium oxysporum , galactomyces geotrichum , and alternaria sp. at sites with lesions. the inoculated fruits were treated with new at 10, 30, and 60 mg liter(-1) active chlorine, with copper oxychloride fungicide, and with sterile distilled water (control) for 3, 5, and 10 min. in the experiment with f. oxysporum , 50 to 80% of the control fruits and 50 ...201628221850
enzyme inhibitory metabolites from endophytic penicillium citrinum isolated from boswellia sacra.fungal endophytes establish an important niche within the host plant through the secretion of chemical constituents. isolation of bioactive metabolites could be a vital source for inhibiting the function of enzymes such as α-glucosidase and urease. the present study aimed to elucidate the potential of endophytes associated with boswellia sacra through bioassay-guided isolation and identification of secondary metabolites with enzyme inhibitory ability. endophytic fungal strains viz. penicillium c ...201728220202
expression and distribution of extensins and agps in susceptible and resistant banana cultivars in response to wounding and fusarium oxysporum.banana fusarium wilt caused by fusarium oxysporum f. sp. cubense (foc) is soil-borne disease of banana (musa spp.) causing significant economic losses. extensins and arabinogalactan proteins (agps) are cell wall components important for pathogen defence. their significance for foc resistance in banana was not reported so far. in this study, two banana cultivars differing in foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and agps aft ...201728218299
rapid and sensitive diagnoses of dry root rot pathogen of chickpea (rhizoctonia bataticola (taub.) butler) using loop-mediated isothermal amplification assay.dry root rot (drr) caused by the fungus rhizoctonia bataticola (taub.) butler, is an emerging disease in chickpea. the disease is often mistaken with other root rots like fusarium wilt, collar rot and black root rot in chickpea. therefore, its timely and specific detection is important. current detection protocols are either based on mycological methods or on protocols involving dna amplification by polymerase chain reaction (pcr). here we report the rapid and specific detection of r. bataticola ...201728218268
draft genome sequence of an isolate of fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt of eggplant.here, we present the genome sequence of an isolate (14004) of fusarium oxysporum f. sp. melongenae, an eggplant pathogen. the final assembly consists of 1,631 scaffolds with 53,986,354 bp (g+c content, 46.4%) and 16,485 predicted genes.201728209821
paenibacillus polymyxa nsy50 suppresses fusarium wilt in cucumbers by regulating the rhizospheric microbial community.paenibacillus polymyxa (p. polymyxa) nsy50, isolated from vinegar residue substrate, suppresses the growth of fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. the aim of the present study was to evaluate the effects of nsy50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to fusarium oxysporum. bacterial and fungal communities were investigated by illumina sequencing of t ...201728198807
potential of burkholderia seminalis tc3.4.2r3 as biocontrol agent against fusarium oxysporum evaluated by mass spectrometry imaging.species of genus burkholderia display different interaction profiles in the environment, causing either several diseases in plants and animals or being beneficial to some plants, promoting their growth, and suppressing phytopathogens. burkholderia spp. also produce many types of biomolecules with antimicrobial activity, which may be commercially used to protect crops of economic interest, mainly against fungal diseases. herein we have applied matrix-assisted laser desorption/ionization mass spec ...201728194740
development of a hydrolysis probe-based real-time assay for the detection of tropical strains of fusarium oxysporum f. sp. cubense race 4.fusarium oxysporum f. sp. cubense (foc) is one of the most important threats to global banana production. strategies to control the pathogen are lacking, with plant resistance offering the only long-term solution, if sources of resistance are available. prevention of introduction of foc into disease-free areas thus remains a key strategy to continue sustainable banana production. in recent years, strains of foc affecting cavendish bananas have destroyed plantations in a number of countries in as ...201728178348
enhancement of seed dehiscence by seed treatment with talaromyces flavus gg01 and gg04 in ginseng (panax ginseng).seed dehiscence of ginseng (panax ginseng c. a. mayer) is affected by moisture, temperature, storage conditions and microbes. several microbes were isolated from completely dehisced seed coat of ginseng cultivars, chunpoong and younpoong at gumsan, korea. we investigated the potential of five talaromyces flavus isolates from the dehiscence of ginseng seed in four traditional stratification facilities. the isolates showed antagonistic activities against fungal plant pathogens, such as cylindrocar ...201728167883
a rare case of subcutaneous fusariosis in an immunocompetent patient.fusarium species can cause diseases in immunocompromised patients, whereas have rarely been reported as pathogens in immunocompetent individuals. fusarium oxysporum and f. solani species complex are the most frequent pathogenic species. primary subcutaneous hyalohyphomycosis due to these filamentous fungi is an extremely rare pathology, especially in immunocompetent patients. only 37 cases of deep fusarium infection in healthy patient have been reported in international literature. we report a c ...201728166614
small rnas regulate the biocontrol property of fluorescent pseudomonas strain psd.the production of biocontrol factors by pseudomonads is reported to be controlled at the post-transcriptional level by the gacs/gaca signal transduction pathway. this involves rna-binding translational repressor proteins, rsma and rsme, and the small regulatory rnas (srnas) rsmx, rsmy, and rsmz. while the former represses genes involved in secondary metabolite production, the latter relieves this repression at the end of exponential growth. we have studied the fluorescent pseudomonas strain psd, ...201728164793
methyl salicylate level increase in flax after fusarium oxysporum infection is associated with phenylpropanoid pathway activation.flax (linum usitatissimum) is a crop plant valued for its oil and fiber. unfortunately, large losses in cultivation of this plant are caused by fungal infections, with fusarium oxysporum being one of its most dangerous pathogens. among the plant's defense strategies, changes in the expression of genes of the shikimate/phenylpropanoid/benzoate pathway and thus in phenolic contents occur. among the benzoates, salicylic acid, and its methylated form methyl salicylate play an important role in regul ...201628163709
development of mesorhizobium ciceri-based biofilms and analyses of their antifungal and plant growth promoting activity in chickpea challenged by fusarium wilt.biofilmed biofertilizers have emerged as a new improved inoculant technology to provide efficient nutrient and pest management and sustain soil fertility. in this investigation, development of a trichoderma viride-mesorhizobium ciceri biofilmed inoculant was undertaken, which we hypothesized, would possess more effective biological nitrogen fixing ability and plant growth promoting properties. as a novel attempt, we selected mesorhizobium ciceri spp. with good antifungal attributes with the assu ...201728148979
application and bioactive properties of cati, a trypsin inhibitor from capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells.during the last few years, a growing number of antimicrobial peptides have been isolated from plants and particularly from seeds. recent results from our laboratory have shown the purification of a new trypsin inhibitor, named cati, from chilli pepper (capsicum annuum l.) seeds. this study aims to evaluate the antifungal activity and mechanism of action of cati on phytopathogenic fungi and detect the presence of protease inhibitors in other species of this genus.201728139827
a new depigmenting-antifungal methylated grindelane from grindelia chiloensis.the new methylated grindelane diterpenoid, 7β-hydroxy-8(17)-dehydrogrindelic acid (1b), together with the known 7α-hydroxy-8(17)-dehydrogrindelic acid (2a), 6-oxogrindelic acid (3a), 4β-hydroxy-6-oxo-19-norgrindelic (4a), 19-hydroxygrindelic acid (5a), 18-hydroxygrindelic acid (6a), 4α-carboxygrindelic acid (7a), 17-hydroxygrindelic acid (8a), 6α-hydroxygrindelic acid (9a), 8,17-bisnor-8-oxagrindelic acid (10a), 7α,8α-epoxygrindelic acid (11a), and strictanonic acid (12a) as methyl esters were o ...201728135782
trichoderma harzianum containing 1-aminocyclopropane-1-carboxylate deaminase and chitinase improved growth and diminished adverse effect caused by fusarium oxysporum in soybean.an isolate, named trichoderma harzianum t-soybean, showed growth-promoting for soybean seedlings and induced resistance to fusarium oxysporum under greenhouse. compared to control soybean seedlings, fresh weight, dry weight, lateral root number, chlorophyll content, root activity and soluble protein of plants pretreated with t-soybean increased, but initial pod height reduced. furthermore, we found that t-soybean inhibited the growth of f. oxysporum by parasitic function. in addition, plate test ...201728135657
synthesis and antifungal activity of novel myrtenal-based 4-methyl-1,2,4-triazole-thioethers.a series of novel myrtenal derivatives bearing 1,2,4-triazole moiety were designed and synthesized by multi-step reactions in an attempt to develop potent antifungal agents. their structures were confirmed by using uv-vis, ftir, nmr, and esi-ms analysis. antifungal activity of the target compounds was preliminarily evaluated by the in vitro method against fusarium oxysporum f. sp. cucumerinum, physalospora piricola, alternaria solani, cercospora arachidicola, and gibberella zeae at 50 µg/ml. com ...201728125042
discovery of fungal denitrification inhibitors by targeting copper nitrite reductase from fusarium oxysporum.the efficient application of nitrogenous fertilizers is urgently required, as their excessive and inefficient use is causing substantial economic loss and environmental pollution. a significant amount of applied nitrogen in agricultural soils is lost as nitrous oxide (n2o) in the environment due to the microbial denitrification process. the widely distributed fungus fusarium oxysporum is a major denitrifier in agricultural soils and its denitrification activity could be targeted to reduce nitrog ...201728117584
novel 1,2,3-triazolium-functionalized starch derivatives: synthesis, characterization, and evaluation of antifungal property.four novel 1,2,3-triazolium-functionalized starch derivatives were synthesized by n-alkylating the precursor starch derivatives with 1,2,3-triazole with iodomethane based on cuprous-catalyzed azide-alkyne cycloaddition (cuaac). the detailed structural characterization was investigated by means of ftir, uv-vis, (1)h nmr, and (13)c nmr spectra. the antifungal activities of starch derivatives against colletotrichum lagenarium, fusarium oxysporum, and watermelon fusarium, were then assayed by hypha ...201728115090
a tomatinase-like enzyme acts as a virulence factor in the wheat pathogen fusarium graminearum.during their interactions with plants, fungal pathogens employ large numbers of pathogenesis-associated molecules including secreted effectors and enzymes that can degrade various defence compounds. however, in many cases, in planta targets of pathogen-produced enzymes remain unknown. we identified a gene in the wheat pathogen fusarium graminearum, encoding a putative enzyme that shows 84% sequence identity to fotom1, a tomatinase produced by the tomato pathogen fusarium oxysporum f. sp. lycoper ...201728109774
structure-activity relationship of α mating pheromone from the fungal pathogen fusarium oxysporum.during sexual development ascomycete fungi produce two types of peptide pheromones termed a and α. the α pheromone from the budding yeast saccharomyces cerevisiae, a 13-residue peptide that elicits cell cycle arrest and chemotropic growth, has served as paradigm for the interaction of small peptides with their cognate g protein-coupled receptors. however, no structural information is currently available for α pheromones from filamentous ascomycetes, which are significantly shorter and share almo ...201728100777
fusaric acid contributes to virulence of fusarium oxysporum on plant and mammalian hosts.fusaric acid (fa) is amongst the oldest identified secondary metabolites produced by fusarium species, known for a long time to display strong phytotoxicity and moderate toxicity to animal cells; however, the cellular targets of fa and its function in fungal pathogenicity remain unknown. here, we investigated the role of fa in fusarium oxysporum, a soil-borne cross-kingdom pathogen that causes vascular wilt on more than 100 plant species and opportunistic infections in humans. targeted deletion ...201728093838
comparative transcriptomics atlases reveals different gene expression pattern related to fusarium wilt disease resistance and susceptibility in two vernicia species.vernicia fordii (tung oil tree) is a promising industrial crop. unfortunately, the devastating fusarium wilt disease has caused its great losses, while its sister species (vernicia montana) is remarkably resistant to this pathogen. however, the genetic mechanisms underlying this difference remain largely unknown. we here generated comparative transcriptomic atlases for different stages of fusarium oxysporum infected vernicia root. the transcriptomes of v. fordii and v. montana were assembled de ...201628083008
molecular evidence for the involvement of a polygalacturonase-inhibiting protein, ghpgip1, in enhanced resistance to verticillium and fusarium wilts in cotton.polygalacturonase-inhibiting protein (pgip), belonging to a group of plant defence proteins, specifically inhibits endopolygalacturonases secreted by pathogens. herein, we showed that purified ghpgip1 is a functional inhibitor of verticillium dahliae and fusarium oxysporum f. sp. vasinfectum, the two fungal pathogens causing cotton wilt. transcription of ghpgip1 was increased in cotton upon infection, wounding, and treatment with defence hormone and h2o2. resistance by ghpgip1 was examined by it ...201728079053
uptake of the fusarium effector avr2 by tomato is not a cell autonomous event.pathogens secrete effector proteins to manipulate the host for their own proliferation. currently it is unclear whether the uptake of effector proteins from extracellular spaces is a host autonomous process. we study this process using the avr2 effector protein from fusarium oxysporum f. sp. lycopersici (fol). avr2 is an important virulence factor that is secreted into the xylem sap of tomato following infection. besides that, it is also an avirulence factor triggering immune responses in plants ...201628066471
tricalcium phosphate solubilization and nitrogen fixation by newly isolated aneurinibacillus aneurinilyticus ckmv1 from rhizosphere of valeriana jatamansi and its growth promotional effect.aneurinibacillus aneurinilyticus strain ckmv1 was isolated from rhizosphere of valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260mg/l), nitrogen fixation (202.91nmolethyleneml(-1)h(-1)), indole-3-acetic acid (iaa) (8.1μg/ml), siderophores (61.60%), hcn (hydrogen cyanide) production and antifungal activity. we investigated the ability of isolate ckmv1 to solubilize insoluble p via mechanism of organic acid production. high-per ...201728063921
down-regulatory effect of thymus vulgaris l. on growth and tri4 gene expression in fusarium oxysporum strains.the aims of this study were to evaluate the efficacy of thymus vulgaris (t. vulgaris) essential oil on the fungal growth and tri4 gene expression in fusarium oxysporum (f. oxysporum) strains. the oil was obtained by water-distillation using a clevenger-type system. the chemical composition of the essential oil was obtained by gas chromatography- mass spectroscopy (gc-ms) and by retention indices. the antifungal activity was evaluated by broth microdilution assay. a quantitative real time rt-pcr ...201728062283
chemical profile and defensive function of the latex of euphorbia peplus.plant latex is an endogenous fluid secreted from highly specialized laticifer cells and has been suggested to act as a plant defense system. the chemical profile of the latex of euphorbia peplus was investigated. a total of 13 terpenoids including two previously unknown diterpenoids, (2s*,3s*,4r*,5r*,6r*,8r*,l1r*,13s*,14s*,15r*, 16r*)-5,8,15-triacetoxy-3-benzoyloxy-11,16-dihydroxy-9-oxopepluane and (2r*,3r*, 4s*,5r*,7s*,8s*,9s*,l3s*,14s*,15r*)-2,5,8,9,14-pentaacetoxy-3-benzoyloxy-15-hydroxy-7-is ...201728062071
a novel peroxidase purified from marsdenia megalantha latex inhibits phytopathogenic fungi mediated by cell membrane permeabilization.an antifungal class iii peroxidase was purified from marsdenia megalantha latex (named mo-pox) using deae-cellulose and gel filtration chromatography on a superose 12 hr 10/30 column. mm-pox has an apparent molecular mass of 67.0kda and a pi of 5.2, shares identity with other peroxidases, and follows michaelis-menten kinetics. it has a high affinity for guaiacol and hydrogen peroxide. the ph and temperature optima for mm-pox were 5.0-7.0 and 60°c, respectively. the catalytic activity of mm-pox w ...201728057569
defense peptide repertoire of stellaria media predicted by high throughput next generation sequencing.being perfectly adapted to diverse environments, chickweed (stellaria media (l.) vill), a ubiquitous garden weed, grows widely in europe and north america. as opposed to the model plants, many weeds, and s. media in particular, have been poorly studied, although they are likely to contain promising components of immunity and novel resistance genes. in this study, for the first time rna-seq analysis of healthy and infected with fusarium oxysporum chickweed seedlings, as well as de novo transcript ...201728038935
correction: transcription factors encoded on core and accessory chromosomes of fusarium oxysporum induce expression of effector genes.[this corrects the article doi: 10.1371/journal.pgen.1006401.].201628033317
proteomic analysis reveals the positive roles of the plant-growth-promoting rhizobacterium nsy50 in the response of cucumber roots to fusarium oxysporum f. sp. cucumerinum inoculation.plant-growth-promoting rhizobacteria (pgpr) can both improve plant growth and enhance plant resistance against a variety of environmental stresses. to investigate the mechanisms that pgpr use to protect plants under pathogenic attack, transmission electron microscopy analysis and a proteomic approach were designed to test the effects of the new potential pgpr strain paenibacillus polymyxa nsy50 on cucumber seedling roots after they were inoculated with the destructive phytopathogen fusarium oxys ...201628018395
leveraging the attributes of mucor hiemalis-derived silver nanoparticles for a synergistic broad-spectrum antimicrobial platform.driven by the need to engineer robust surface coatings for medical devices to prevent infection and sepsis, incorporation of nanoparticles has surfaced as a promising avenue to enhance non-fouling efficacy. microbial synthesis of such nanoscale metallic structures is of substantive interest as this can offer an eco-friendly, cost-effective, and sustainable route for further development. here we present a mucor hiemalis-derived fungal route for synthesis of silver nanoparticles, which display sig ...201628018316
the tomato i gene for fusarium wilt resistance encodes an atypical leucine-rich repeat receptor-like protein whose function is nevertheless dependent on sobir1 and serk3/bak1.we have identified the tomato i gene for resistance to the fusarium wilt fungus fusarium oxysporum f. sp. lycopersici (fol) and show that it encodes a membrane-anchored leucine-rich repeat receptor-like protein (lrr-rlp). unlike most other lrr-rlp genes involved in plant defence, the i gene is not a member of a gene cluster and contains introns in its coding sequence. the i gene encodes a loopout domain larger than those in most other lrr-rlps, with a distinct composition rich in serine and thre ...201727995670
bioactive secondary metabolites with multiple activities from a fungal endophyte.in order to replace particularly biohazardous nematocides, there is a strong drive to finding natural product-based alternatives with the aim of containing nematode pests in agriculture. the metabolites produced by the fungal endophyte fusarium oxysporum 162 when cultivated on rice media were isolated and their structures elucidated. eleven compounds were obtained, of which six were isolated from a fusarium spp. for the first time. the three most potent nematode-antagonistic compounds, 4-hydroxy ...201727990770
non-canonical helitrons in fusarium oxysporum.helitrons are eukaryotic rolling circle transposable elements that can have a large impact on host genomes due to their copy-number and their ability to capture and copy genes and regulatory elements. they occur widely in plants and animals, and have thus far been relatively little investigated in fungi.201627990178
antibacterial combination of oleoresin from copaifera multijuga hayne and biogenic silver nanoparticles towards streptococcus agalactiae.streptococcus agalactiae (group b streptococcus - gbs) remains a leading cause of neonatal infections and an important cause of invasive infections in adults with underlying conditions.201627978809
controlled biosynthesis of silver nanoparticles using nitrate reductase enzyme induction of filamentous fungus and their antibacterial evaluation.the controlled synthesis of silver nanoparticles (agnps) using cell-free filtrate of fusarium oxysporum fungus was investigated. the effect of fungal incubation period on nanoparticle formation and nitrate reductase enzyme activity was studied using uv-visible spectroscopy and harley assay, respectively. the highest agnp formation was observed in the cell-free filtrate of biomass harvested at the early stationary phase where the nr enzyme activity is the maximum. mixing of the cell-free filtrate ...201627966375
stilbene derivatives from photorhabdus temperata sn259 and their antifungal activities against phytopathogenic fungi.chemical investigation of an insect pathogenic enterobacterium, photorhabdus temperata sn259, led to the isolation and identification of seven metabolites, which include three new compounds, 3-hydroxy-2-isopropyl-5-phenethylphenyl carbamate, 1, 2-(1-hydroxypropan-2-yl)-5-[2-phenylethyl]benzene-1,3-diol, 2, 2-(1-hydroxypropan-2-yl)-5-[(e)-2-phenylethenyl]benzene-1,3-diol, 3, and four known metabolites (4-7). their structures were elucidated on the basis of ms and nmr data and by comparison with t ...201727960253
effects of iron and boron combinations on the suppression of fusarium wilt in banana.the effects of mineral nutrient on banana wilt disease, which are the result of a competitive relationship between host plants and pathogens, can affect the interactions of plants with microorganisms. to investigate the mineral nutrient effect, hydroponic experiments were conducted in glasshouse containing combinations of low, medium, and high iron (fe) and boron (b) concentrations, followed by pathogen inoculation. high fe and b treatment significantly reduced the disease index and facilitated ...201627941854
recombinant expression, characterization, and application of a phospholipase b from fusarium oxysporum.in this study, a gene encoding a putative lipase from fusarium oxysporum was optimized via codon optimization and expressed in pichia pastoris km71. the gene product was identified as a phospholipase b (plb). the engineered p. pastoris was further cultured in a 3.6-l bioreactor. after optimization of the induction conditions, this system produced 6.6mgml(-1) protein and 6503.8uml(-1) plb activity in the culture medium. efficient expression of this plb in p. pastoris should reduce the costs of pr ...201727940286
races of the celery pathogen fusarium oxysporum f. sp. apii are polyphyletic.fusarium oxysporum species complex (fosc) isolates were obtained from celery with symptoms of fusarium yellows between 1993 and 2013 primarily in california. virulence tests and a two-gene dataset from 174 isolates indicated that virulent isolates collected before 2013 were a highly clonal population of f. oxysporum f. sp. apii race 2. in 2013, new highly virulent clonal isolates, designated race 4, were discovered in production fields in camarillo, california. long-read illumina data were used ...201727938244
identification of root-secreted compounds involved in the communication between cucumber, the beneficial bacillus amyloliquefaciens, and the soil-borne pathogen fusarium oxysporum.colonization of plant growth-promoting rhizobacteria (pgpr) is critical for exerting their beneficial effects on the plant. root exudation is a major factor influencing the colonization of both pgpr and soil-borne pathogens within the root system. however, the tripartite interaction of pgpr, plant roots, and soil-borne pathogens is poorly understood. we screened root exudates for signals that mediate tripartite interactions in the rhizosphere. in a split-root system, we found that root colonizat ...201727937752
antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa.plants harbor endophytes with potential bioactivity. markhamia tomentosa (benth) k. schum ex. engl. (bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities.201727937112
rna-seq transcriptome response of flax (linum usitatissimum l.) to the pathogenic fungus fusarium oxysporum f. sp. lini.fusarium oxysporum f. sp. lini is a hemibiotrophic fungus that causes wilt in flax. along with rust, fusarium wilt has become an important factor in flax production worldwide. resistant flax cultivars have been used to manage the disease, but the resistance varies, depending on the interactions between specific cultivars and isolates of the pathogen. this interaction has a strong molecular basis, but no genomic information is available on how the plant responds to attempted infection, to inform ...201627933082
proliferation of the biocontrol agent fusarium oxysporum f. sp. strigae and its impact on indigenous rhizosphere fungal communities in maize under different agro-ecologies.our objectives were to (1) monitor the proliferation of the biocontrol agent (bca) fusarium oxysporum f. sp. strigae strain "foxy-2", an effective soil-borne bca against the parasitic weed striga hermonthica, in the rhizosphere of maize under different agro-ecologies, and (2) investigate its impact on indigenous rhizosphere fungal community abundance and composition. field experiments were conducted in busia and homa bay districts in western kenya during two cropping seasons to account for effec ...201627928553
sequencing of individual chromosomes of plant pathogenic fusarium oxysporum.a small chromosome in reference isolate 4287 of f. oxysporum f. sp. lycopersici (fol) has been designated as a 'pathogenicity chromosome' because it carries several pathogenicity related genes such as the secreted in xylem (six) genes. sequence assembly of small chromosomes in other isolates, based on a reference genome template, is difficult because of karyotype variation among isolates and a high number of sequences associated with transposable elements. these factors often result in misassemb ...201727919652
chemical profiling, antimicrobial and insecticidal evaluations of polygonum hydropiper l.the emergence of multidrug resistant (mdr) pathogens is of great concern to the global health community. our ability to effectively treat diseases is based on the discovery of potent drugs for the treatment of these challenging diseases. traditional medicines are one of the major sources for the discovery of safe, effective and economical drug candidates. in order to validate its antibacterial, antifungal and insecticidal potentials with respect to traditional uses, we have screened for the firs ...201627919287
identification, characterization and phylogenetic analysis of antifungal trichoderma from tomato rhizosphere.the use of trichoderma isolates with efficient antagonistic activity represents a potentially effective and alternative disease management strategy to replace health hazardous chemical control. in this context, twenty isolates were obtained from tomato rhizosphere and evaluated by their antagonistic activity against four fungal pathogens (fusarium oxysporum f. sp. lycopersici, alternaria alternata, colletotrichum gloeosporoides and rhizoctonia solani). the production of extracellular cell wall d ...201627917337
divergent expression patterns in two vernicia species revealed the potential role of the hub gene vmap2/erf036 in resistance to fusarium oxysporum in vernicia montana.tung oil tree (vernicia fordii) is a promising industrial oil crop; however, this tree is highly susceptible to fusarium wilt disease. conversely, vernicia montana is resistant to the pathogen. the apetala2/ethylene-responsive element binding factor (ap2/erf) transcription factor superfamily has been reported to play a significant role in resistance to fusarium oxysporum. in this study, comprehensive analysis identified 75 and 81 putative vf/vmap2/erf transcription factor-encoding genes in v. fo ...201627916924
multiple evolutionary trajectories have led to the emergence of races in fusarium oxysporum f. sp. lycopersici.race 1 isolates of fusarium oxysporum f. sp. lycopersici (fol) are characterized by the presence of avr1 in their genomes. the product of this gene, avr1, triggers resistance in tomato cultivars carrying resistance gene i in fol race 2 and race 3 isolates, avr1 is absent, and hence they are virulent on tomato cultivars carrying i in this study, we analyzed an approximately 100-kb genomic fragment containing the avr1 locus of fol race 1 isolate 004 (fol004) and compared it to the sequenced genome ...201727913420
insights into the regulation of rhizosphere bacterial communities by application of bio-organic fertilizer in pseudostellaria heterophylla monoculture regime.the biomass and quality of pseudostellariae heterophylla suffers a significant decline under monoculture. since rhizosphere miobiome plays crucial roles in soil health, deep pyrosequencing combined with qpcr was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. the results showed compared with the 1st-year planted (fp), 2nd-year monoculture of p. heterophylla (sp) led to a significant decline in yield and resulted in a s ...201627899917
evaluation of corneal cross-linking for treatment of fungal keratitis: using confocal laser scanning microscopy on an ex vivo human corneal model.some previous reports have established the use of photoactivated chromophore-induced corneal cross-linking (pack-cxl) in treating fungal keratitis. the results of these case reports have often been conflicting. to systematically study the effect of pack-cxl in the management of fusarium keratitis, we have developed an ex vivo model of human corneal infection using eye-banked human corneas.201627898982
effects of polysaccharide elicitors from endophytic fusarium oxysporum fat9 on the growth, flavonoid accumulation and antioxidant property of fagopyrum tataricum sprout cultures.the purpose of this study was to evaluate the effects of four different fungal polysaccharides, named water-extracted mycelia polysaccharide (wps), sodium hydroxide-extracted mycelia polysaccharide (sps), hydrochloric-extracted mycelia polysaccharide (aps), and exo-polysaccharide (eps) obtained from the endophytic fusarium oxysporum fat9 on the sprout growth, flavonoid accumulation, and antioxidant capacity of tartary buckwheat. without visible changes in the appearance of the sprouts, the exoge ...201627897983
antifungal activities of selected essential oils against fusarium oxysporum f. sp. lycopersici 1322, with emphasis on syzygium aromaticum essential oil.the antifungal effects of four essential oils viz., clove (syzygium aromaticum), lemongrass (cymbopogon citratus), mint (mentha × piperita) and eucalyptus (eucalyptus globulus) were evaluated against wilt causing fungus, fusarium oxysporum f. sp. lycopersici 1322. the inhibitory effect of oils showed dose-dependent activity on the tested fungus. most active being the clove oil, exhibiting complete inhibition of mycelial growth and spore germination at 125 ppm with ic50 value of 18.2 and 0.3 ppm, ...201727876218
control of wilt and rot pathogens of tomato by antagonistic pink pigmented facultative methylotrophic delftia lacustris and bacillus spp.the studies on the biocontrol potential of pink pigmented facultative methylotrophic (ppfm) bacteria other than the genus methylobacterium are scarce. in the present study, we report three facultative methylotrophic isolates; ppo-1, ppt-1, and ppb-1, respectively, identified as delftia lacustris, bacillus subtilis, and bacillus cereus by 16s rrna gene sequence analysis. hemolytic activity was tested to investigate the potential pathogenicity of isolates to plants and humans, the results indicate ...201627872630
efficient continuous biosynthesis of silver nanoparticles by activated sludge micromycetes with enhanced tolerance to metal ion toxicity.the method for producing agnps by granules of activated sludge micromycetes with enhanced tolerance to metal ion toxicity - penicillium glabrum, fusarium nivale and fusarium oxysporum has been developed; the optimum conditions for agnp biosynthesis being found: the ag(+) ion concentration, duration of the contact of microbial cells with silver ions, a growth phase of microorganisms, medium composition, a рн value, mixing conditions, and also lighting intensity. the effect of cl(-), so4(2-) and h ...201627866609
bacillus species (bt42) isolated from coffea arabica l. rhizosphere antagonizes colletotrichum gloeosporioides and fusarium oxysporum and also exhibits multiple plant growth promoting activity.colletotrichum and fusarium species are among pathogenic fungi widely affecting coffea arabica l., resulting in major yield loss. in the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing colletotrichum gloeosporioides and fusarium oxysporum as well as promotes plant growth.201627863465
heat shock transcription factors in banana: genome-wide characterization and expression profile analysis during development and stress response.banana (musa acuminata) is one of the most popular fresh fruits. however, the rapid spread of fungal pathogen fusarium oxysporum f. sp. cubense (foc) in tropical areas severely affected banana growth and production. thus, it is very important to identify candidate genes involved in banana response to abiotic stress and pathogen infection, as well as the molecular mechanism and possible utilization for genetic breeding. heat stress transcription factors (hsfs) are widely known for their common in ...201627857174
elucidation of biocontrol mechanisms of trichoderma harzianum against different plant fungal pathogens: universal yet host specific response.in the present study, different transcripts of trichoderma harzianum thhp-3 were evaluated for their response against four fungal pathogens fusarium oxysporum, colletotrichum capsici, colletotrichum truncatum and gloesercospora sorghi using rt-qpcr. the time course study of t. harzianum transcripts related to signal transduction, lytic enzymes, secondary metabolites and various transporters revealed variation in expression against four fungal pathogens. in a broader term, the transcripts were up ...201727856319
transcription factors encoded on core and accessory chromosomes of fusarium oxysporum induce expression of effector genes.proteins secreted by pathogens during host colonization largely determine the outcome of pathogen-host interactions and are commonly called 'effectors'. in fungal plant pathogens, coordinated transcriptional up-regulation of effector genes is a key feature of pathogenesis and effectors are often encoded in genomic regions with distinct repeat content, histone code and rate of evolution. in the tomato pathogen fusarium oxysporum f. sp. lycopersici (fol), effector genes reside on one of four acces ...201627855160
antifungal lipopeptides produced by bacillus sp. fjat-14262 isolated from rhizosphere soil of the medicinal plant anoectochilus roxburghii.this study aimed to develop biocontrol bacillus and explore bacterial biocontrol substances. according to the blood agar test, strain fjat-14262 was screened as a biosurfactant-producer. the biosurfactant-producing ability of fjat-14262 was further confirmed by the oil spreading tests because of its amphipathic character. furthermore, its fermentation supernatant could decrease the surface tension from 74.1 to 32.7 mn m(-1). fourier transform infrared spectroscopy (ft-ir) analysis indicated that ...201627854037
genome wide transcriptome profiling of fusarium oxysporum f sp. ciceris conidial germination reveals new insights into infection-related genes.vascular wilt caused by fusarium oxysporum f. sp. ciceris (foc) is a serious disease of chickpea (cicer arietinum l.) accounting for approximately 10-15% annual crop loss. the fungus invades the plant via roots, colonizes the xylem vessels and prevents the upward translocation of water and nutrients. infection is initiated by conidia that invade the host tissue often by penetration of intact epidermal cells. here, we report the characterization of the transcriptome of foc sequenced using illumin ...201627853284
phytotoxic, antifungal and immunosuppressive metabolites from aspergillus terreus qt122 isolated from the gut of dragonfly.insect gut microbes have been considered as a resource for bioactive metabolites. the aim of this study was to characterize the compounds of a fungus aspergillus terreus qt122 associated with the gut of dragonfly. five main phytotoxic, antifungal, and immunosuppressive substances were isolated from the fungus qt122. the structures of such compounds were identified as emodin (1), 1-methyl emodin (2), terrein (3), methyl 6-acetyl-4-methoxy-7,8-dihydroxynaphthalene-2-carboxylate (4), and dihydrogeo ...201727844127
transcriptome analysis of the fungal pathogen fusarium oxysporum f. sp. medicaginis during colonisation of resistant and susceptible medicago truncatula hosts identifies differential pathogenicity profiles and novel candidate effectors.pathogenic members of the fusarium oxysporum species complex are responsible for vascular wilt disease on many important crops including legumes, where they can be one of the most destructive disease causing necrotrophic fungi. we previously developed a model legume-infecting pathosystem based on the reference legume medicago truncatula and a pathogenic f. oxysporum forma specialis (f. sp.) medicaginis (fom). to dissect the molecular pathogenicity arsenal used by this root-infecting pathogen, we ...201627809762
a gbs-snp-based linkage map and quantitative trait loci (qtl) associated with resistance to fusarium oxysporum f. sp. niveum race 2 identified in citrullus lanatus var. citroides.a major qtl for resistance to fusarium oxysporum f. sp. niveum race 2 was mapped to a narrow 1.2 mb interval using a high-density gbs-snp linkage map, the first map of citrullus lanatus var. citroides. fusarium wilt, a fungal disease caused by fusarium oxysporum f. sp. niveum (fon), devastates watermelon crop production worldwide. several races, which are differentiated by host range, of the pathogen exist. resistance to fon race 2, a particularly virulent strain prevalent in the united states, ...201727803951
biological characteristics and antimicrobial activity of endophytic streptomyces sp. tqr12-4 isolated from elite citrus nobilis cultivar ham yen of vietnam.ham yen orange (citrus nobilis lour) is the highly valuable commercial fruit of vietnam. with the blooming of fruit production and farming area, this specialty crop is facing threats from several serious diseases; therefore the search for new effective biocontrollers is required to prevent the existing excessive use of fertilizers and plant protection chemicals. endophytic actinomycetes are of great scientific interest due to their high potential of application in agriculture and pharmaceutical ...201627795709
antifungal, anticancer and aminopeptidase inhibitory potential of a phenazine compound produced by lactococcus bsn307.a bioactive compound was purified from the culture medium of a new strain of lactococcus bsn307 by solvent extraction followed by chromatographic techniques. this bioactive compound was identified to belong to phenazine class of compounds by ms, nmr and ftir. the phenazine compound showed antifungal activity against aspergillus niger, penicillium chrysogenum as well as fusarium oxysporum by disc diffusion assay in addition to antioxidant potential as demonstrated by dpph scavenging assay. the co ...201627784936
koninginins r-s from the endophytic fungus trichoderma koningiopsis.two new metabolites named koninginins r-s (1-2) were isolated from the culture of trichoderma koningiopsis yim ph30002. their chemical structures were elucidated by the extensive spectroscopic analysis. these isolated compounds showed certain antifungal activities against phytopathogens, fusarium flocciferum and fusarium oxysporum.201727784167
improved catalytic and antifungal activities of bacillus thuringiensis cells with surface display of chi9602δsp.the cell-surface display of chitinase chi9602δsp was constructed on bacillus thuringiensis bmb171 by using two repeat n-terminal regions of autolysin (mbgn)2 as the anchoring motif. this study aimed to prepare a chitinolytic whole-cell b. thuringiensis biocatalyst with improved catalytic and antifungal activities.201727782360
preparation, characterization, and bio-efficacy evaluation of controlled release carbendazim-loaded polymeric nanoparticles.synthesis and controlled release study of polymeric nanoformulation of carbendazim (2-benzimidazole carbamic acid methyl ester) using chitosan and pectin is reported in this article. the formulation was subjected to morphological, physiological, in vitro fungicide release and bio-efficacy evaluation studies. the average size of nanoparticles was found to be in the range of 70-90 nm as confirmed by transmission electron microscopy. the in vitro fungicide release of nanoformulated carbendazim was ...201727761863
naphthalene acetic acid potassium salt (naa-k(+)) affects conidial germination, sporulation, mycelial growth, cell surface morphology, and viability of fusarium oxysporum f. sp. radici-lycopersici and f. oxysporum f. sp. cubense in vitro.the response to exogenous addition of naphthalene acetic acid potassium salt (naa-k(+)) to fusarium oxysporum f. sp radici-lycopersici atcc 60095 and f. oxysporum f. sp. cubense isolated from michoacan mexico soil is reported. the in vitro study showed that naa-k(+) might be effective in the control of fusarium oxysporum. exogenous application of naa-k(+) affected both spores and mycelium stages of the fungi. viability testing using acridine orange and propidium iodide showed that naa-k(+) posse ...201627754669
streptomyces luozhongensis sp. nov., a novel actinomycete with antifungal activity and antibacterial activity.a novel actinomycete strain, designated trm 49605(t), was isolated from a desert soil sample from lop nur, xinjiang, north-west china, and characterised using a polyphasic taxonomic approach. the strain exhibited antifungal activity against the following strains: saccharomyces cerevisiae, curvularia lunata, aspergillus flavus, aspergillus niger, fusarium oxysporum, penicillium citrinum, candida albicans and candida tropicalis; antibacterial activity against bacillus subtilis, staphylococcus epid ...201727752797
synthesis of novel tetrazole derivatives and evaluation of their antifungal activity.with the appearance of the antifungal resistance, novel antifungal agents need to be identified. in this context new 2,5-disubstituted tetrazole derivatives containing benzothiazole, benzoxazole or phenylsulfonyl moiety were synthesized by n-alkylation of aryltetrazole with 2-[(3-chloropropyl)sulfanyl]-1,3-benzothiazole or 2-[(3-chloropropyl)sulfanyl]-1,3-benzoxazole and michael-type addition of aryltetrazole to phenyl vinyl sulfone. the chemical structures of the synthesized compounds were conf ...201627745991
identification and evidence of positive selection upon resistance gene analogs in cotton (gossypium hirsutum l.).upland cotton (gossypium hirsutum l.) is an important fiber crop species, which is intensively plagued by a plethora of phytopathogenic fungi such as fusarium oxysporum f. sp. vasinfectum (fov) causing severe wilt disease. resistance gene analogs (rgas) are the largest class of potential resistance (r) genes depicting highly conserved domains and structures in plants. additionally, rgas are pivotal components of breeding projects towards host disease resistance, serving as useful functional mark ...201627729728
synthesis and antifungal activity against fusarium oxysporum of some brassinin analogs derived from l-tryptophan: a dft/b3lyp study on the reaction mechanism.an efficient methodology to obtain novel antifungal analogs of brassinin 1 is described. starting from l-tryptophan 2, n,n'-dialkylthiourea 4, 4-[(1h-indol-3-yl)methylene]-2-sulfanylidene-1,3-thiazolidin-5-one 5 and alkyl (2s)-3-(1h-indol-3-yl)-2-{[(alkylsulfanyl)carbonothioyl]amino}propanoate 6 type compounds were obtained as main products in different ratios depending on the reaction conditions via a tandem dithiocarbamate formation and michael addition reaction. in order to understand the dep ...201627727186
biocontrol agent fusarium oxysporum f.sp. strigae has no adverse effect on indigenous total fungal communities and specific amf taxa in contrasting maize rhizospheres.we studied the effects of fusarium oxysporum f.sp. strigae (fos), a soil-borne biocontrol agent (bca) against striga hermonthica, on total fungal and arbuscular mycorrhizal fungal (amf) taxa in rhizospheres of maize in both clayey and sandy soil. effects of fos-bca 'foxy-2' were evaluated against (1) s. hermonthica presence, and (2) organic fertilization with tithonia diversifolia residues at 14, 28 and 42 d after 'foxy-2' inoculation, via dna-based quantitative pcr and trflp fingerprinting. in ...201627721900
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