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preparation and characterization of novel cationic chitosan derivatives bearing quaternary ammonium and phosphonium salts and assessment of their antifungal properties.chitosan is an abundant and renewable polysaccharide, its derivatives exhibit attractive bioactivities and the wide applications in various biomedical fields. in this paper, two novel cationic chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized via trimethylation, chloride acetylation, and quaternization with tricyclohexylphosphine and triphenylphosphine. the structures and properties of synthesized products in the reactions were characterized by ftir sp ...201728858241
structure-function analysis of the fusarium oxysporum avr2 effector allows uncoupling of its immune-suppressing activity from recognition.plant pathogens employ effector proteins to manipulate their hosts. fusarium oxysporum f. sp. lycopersici (fol), the causal agent of tomato wilt disease, produces effector protein avr2. besides being a virulence factor, avr2 triggers immunity in i-2 carrying tomato (solanum lycopersicum). fol strains that evade i-2 recognition carry point mutations in avr2 (e.g. avr2(r45h) ), but retain full virulence. here we investigate the virulence function of avr2 and determine its crystal structure. transg ...201728857169
antioxidant and antifungal activity of selected medicinal plant extracts against phytopathogenic fungi.medicinal plants are used by many ethnic groups as a source of medicine for the treatment of various ailments in both humans and domestic animals. these plants produce secondary metabolites that have antimicrobial properties, thus screening of medicinal plants provide another alternative for producing chemical fungicides that are relatively non-toxic and cost-effective.201628852739
the powdery mildew-resistant arabidopsis mlo2 mlo6 mlo12 triple mutant displays altered infection phenotypes with diverse types of phytopathogens.arabidopsis thaliana mlo2 mlo6 mlo12 triple mutant plants exhibit complete immunity against infection by otherwise virulent obligate biotrophic powdery mildew fungi such as golovinomyces orontii. while this phenotype is well documented, the interaction profile of the triple mutant with other microbes is underexplored and incomplete. here, we thoroughly assessed and quantified the infection phenotypes of two independent powdery mildew-resistant triple mutant lines with a range of microbes. these ...201728839137
a mobile pathogenicity chromosome in fusarium oxysporum for infection of multiple cucurbit species.the genome of fusarium oxysporum (fo) consists of a set of eleven 'core' chromosomes, shared by most strains and responsible for housekeeping, and one or several accessory chromosomes. we sequenced a strain of fo f.sp. radicis-cucumerinum (forc) using pacbio smrt sequencing. all but one of the core chromosomes were assembled into single contigs, and a chromosome that shows all the hallmarks of a pathogenicity chromosome comprised two contigs. a central part of this chromosome contains all identi ...201728831051
identification and antifungal activity of compounds from the mangrove endophytic fungus aspergillus clavatus r7.two new coumarin derivatives, 4,4'-dimethoxy-5,5'-dimethyl-7,7'-oxydicoumarin (1), 7-(γ,γ-dimethylallyloxy)-5-methoxy-4-methylcoumarin (2), a new chromone derivative, (s)-5-hydroxy-2,6-dimethyl-4h-furo[3,4-g]benzopyran-4,8(6h)-dione (5), and a new sterone derivative, 24-hydroxylergosta-4,6,8(14),22-tetraen-3-one (6), along with two known bicoumarins, kotanin (3) and orlandin (4), were isolated from an endophytic fungus aspergillusclavatus (collection no. r7), isolated from the root of myoporum b ...201728825634
comparison of transcriptome profiles by fusarium oxysporum inoculation between fusarium yellows resistant and susceptible lines in brassica rapa l.resistant and susceptible lines in brassica rapa have different immune responses against fusarium oxysporum inoculation. fusarium yellows caused by fusarium oxysporum f. sp. conglutinans (foc) is an important disease of brassicaceae; however, the mechanism of how host plants respond to foc is still unknown. by comparing with and without foc inoculation in both resistant and susceptible lines of chinese cabbage (brassica rapa var. pekinensis), we identified differentially expressed genes (degs) b ...201728819684
pathological interrelations of soil-borne diseases in cucurbits caused by fusarium species and meloidogyne incognita.pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by fusarium isolates (fi) and the root-knot nematode (rkn), meloidogyne incognita were characterized by the fusarium disease severity index (di), rkn gall index (gi) and eggmass index (ei) in inoculation tests using fi and rkn. virulence of fi as determined by di at 4 weeks after inoculation was mostly in the higher order of fusarium proliferatum f6, f5 and fusarium oxys ...201728811758
investigating the diversity of effector genes in the banana pathogen, fusarium oxysporum f.sp. cubense, reveals evidence of horizontal gene transfer.it is hypothesised that the virulence of phytopathogenic fungi is mediated through the secretion of small effector proteins that interfere with the defence responses of the host plant. in fusarium oxysporum, one family of effectors, the secreted in xylem (six) genes, have been identified. we sought to characterise the diversity and evolution of the six genes in the banana-infecting lineages of f. oxysporum f.sp. cubense (foc). whole-genome sequencing data was generated for the 23 genetic lineage ...201728802020
rnai-mediated silencing of map kinase signalling genes (fmk1, hog1, and pbs2) in fusarium oxysporum reduces pathogenesis on tomato plants.fusarium oxysporum is a soil-borne plant fungal pathogen, and causes colossal losses in several crop plants including tomato. effective control measures include the use of harmful fungicides and resistant cultivars, but these methods have shown limited success. conventional methods to validate fungal pathogenic genes are labour intensive. therefore, an alternative strategy is required to efficiently characterize unknown pathogenic genes. rna interference (rnai) has emerged as a potential tool to ...201728800849
chickpea-fusarium oxysporum interaction transcriptome reveals differential modulation of plant defense strategies.fusarium wilt is one of the major biotic stresses reducing chickpea productivity. the use of wilt-resistant cultivars is the most appropriate means to combat the disease and secure productivity. as a step towards understanding the molecular basis of wilt resistance in chickpea, we investigated the transcriptomes of wilt-susceptible and wilt-resistant cultivars under both fusarium oxysporum f.sp. ciceri (foc) challenged and unchallenged conditions. transcriptome profiling using longsage provided ...201728798320
synthesis, characterization and antifungal activity of coumarin-functionalized chitosan derivatives.four novel coumarin-functionalized chitosan derivatives 4a-4d were synthesized via condensation reactions of thiosemicarbazide chitosan with coumarin derivatives. their structures were confirmed by ft-ir, (13)c nmr, xrd and elemental analysis. their antifungal activities against three kinds of phytopathogens, alternaria solani sorauer (a. solani), fusarium oxysporum f.sp. vasinfectum (f. oxysporum) and fusarium moniliforme (f. moniliforme), were tested using the mycelial growth rate in vitro at ...201728797818
nonsteroidal anti-inflammatory drugs (nsaids) inhibit the growth and reproduction of chaetomium globosum and other fungi associated with water-damaged buildings.indoor mold due to water damage causes serious human respiratory disorders, and the remediation to homes, schools, and businesses is a major expense. prevention of mold infestation of building materials would reduce health problems and building remediation costs. nonsteroidal anti-inflammatory drugs (nsaids) inhibit yeasts and a limited number of filamentous fungi. the purpose of this research was to determine the possible inhibitory activity of nonsteroidal anti-inflammatory drugs (nsaids) on g ...201728795317
in vitro antimicrobial activities and mechanism of 1-octen-3-ol against food-related bacteria and pathogenic fungi.1-octen-3-ol, known as mushroom alcohol, is a natural product extracted from fungi and plants. its antimicrobial activities against five common food-related bacteria and two pathogenic fungi were evaluated in this paper, including staphylococcus aureus, bacillus subtilis, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, fusarium tricinctum and fusarium oxysporum. the results showed that 1-octen-3-ol had a strong antibacterial activity against the tested bacteria, especially ...201728794307
rna sequencing reveals that endoplasmic reticulum stress and disruption of membrane integrity underlie dimethyl trisulfide toxicity against fusarium oxysporum f. sp. cubense tropical race 4.fusarium wilt of banana, a destructive disease that affects banana production, is caused by fusarium oxysporum f. sp. cubense tropical race 4 (foc tr4). in a previous study, we confirmed the strong inhibitory effects of chinese leek (allium tuberosum) on the incidence of this disease. sulfur compounds are the primary antifungal constituents of chinese leek. among these, dimethyl trisulfide (dt) was the most abundant and exhibited the strongest inhibition of foc tr4 growth and development. in the ...201728790985
bacterial diseases of bananas and enset: current state of knowledge and integrated approaches toward sustainable management.bacterial diseases of bananas and enset have not received, until recently, an equal amount of attention compared to other major threats to banana production such as the fungal diseases black leaf streak (mycosphaerella fijiensis) and fusarium wilt (fusarium oxysporum f. sp. cubense). however, bacteria cause significant impacts on bananas globally and management practices are not always well known or adopted by farmers. bacterial diseases in bananas and enset can be divided into three groups: (1) ...201728785275
c-coordinated o-carboxymethyl chitosan metal complexes: synthesis, characterization and antifungal efficacy.a novel type of o-carboxymethyl chitosan schiff bases (o-cspx) was synthesized via a condensation reaction. after the coordination reaction of cupric ions, zinc ions and nickel ions, metal complexes (o-cspx-m) were achieved. the theoretical structure of o-cspx-m calculated by gaussian 09 reveals that the copper ions and nickel ions underwent dsp(2) hybridization, the zinc ions underwent sp(3) hybridization, and they all coordinated by the carbon atom in the p-π conjugate group. then, the structu ...201728774807
synthesis of cdse quantum dots using fusarium oxysporum.cdse quantum dots are often used in industry as fluorescent materials. in this study, cdse quantum dots were synthesized using fusarium oxysporum. the cadmium and selenium concentration, ph, and temperature for the culture of f. oxysporum (fusarium oxysporum) were optimized for the synthesis, and the cdse quantum dots obtained from the mycelial cells of f. oxysporum were observed by transmission electron microscopy. ultra-thin sections of f. oxysporum showed that the cdse quantum dots were preci ...201628773975
evaluation of multifarious plant growth promoting traits, antagonistic potential and phylogenetic affiliation of rhizobacteria associated with commercial tea plants grown in darjeeling, india.plant growth promoting rhizobacteria (pgpr) are studied in different agricultural crops but the interaction of pgpr of tea crop is not yet studied well. in the present study, the indigenous tea rhizobacteria were isolated from seven tea estates of darjeeling located in west bengal, india. a total of 150 rhizobacterial isolates were screened for antagonistic activity against six different fungal pathogens i.e. nigrospora sphaerica (kj767520), pestalotiopsis theae (itcc 6599), curvularia eragostid ...201728771547
identification and functionality prediction of pathogenesis-related protein 1 from legume family.the production and accumulation of pathogenesis-related (pr) proteins in plants is one of the important responses to biotic and abiotic stress. large number of identified pr proteins has been categorized into 17 functional families based on their structure, phylogenetics, and biological activities. however, they are not widely studied in legume crops. using 29 pr1 proteins from arabidopsis thaliana, as query, here we have predicted 92 candidate pr1 proteins through the psi-blast and hmmer progra ...201728762578
disease resistance to multiple fungal and oomycete pathogens evaluated using a recombinant inbred line population in pepper.incorporating disease resistance into cultivars is a primary focus of modern breeding programs. resistance to pathogens is often introgressed from landrace or wild individuals with poor fruit quality into commercial-quality cultivars. sites of multiple disease resistance (mdr) are regions or "hotspots" of the genome with closely linked genes for resistance to different pathogens that could enable rapid incorporation of resistance. an f2-derived f6 recombinant inbred line population from a cross ...201728762287
chemical composition and antifungal activity of the essential oil of zhumeria majdae, heracleum persicum and eucalyptus sp. against some important phytopathogenic fungi.application of essential oils of medicinal plants is considered a safe and acceptable method for plant disease management to protect plants from pathogenic microorganisms. thus, in recent study, essential oils (eos) from zhumeria majdae, heracleum persicum (two iranian endemic plants) and eucalyptus sp. were assayed for their antifungal potential against ten phytopathogenic fungi, including fusarium graminearum, fusarium asiaticum, fusarium redolens f.sp. dianthus, fusarium verticillioides, fusa ...201728757068
mitochondrial molecular markers for resistance to bayoud disease in date palm.bayoud disease, caused by fusarium oxysporum f. sp. albedinis, is a very serious and destructive disease to date palm. screening of date palm germplasm for resistance to bayoud disease is a crucial step to avoid or alleviate the disease consequences. fortunately, it was discovered that there are two mitochondrial plasmid-like dna molecules associated with susceptibility or resistance to bayoud disease. in this chapter, we present a fast, simple, and reliable technique to screen date palm germpla ...201728755230
comparative agronomic performance and reaction to fusarium wilt of lens culinaris × l. orientalis and l. culinaris × l. ervoides derivatives.the development of transgressive phenotype in the segregating populations has been speculated to contribute to niche divergence of hybrid lineages, which occurs most frequently at larger genetic distances. wild lens species are considered to be more resistant against major biotic and abiotic stresses than that of the cultivated species. in the present study, we assessed the comparative agronomic performance of lentil (lens culinaris subsp. culinaris) inter-sub-specific (l. culinaris subsp. orien ...201728751897
elucidation of the biosynthetic pathway of cis-jasmone in lasiodiplodia theobromae.in plants, cis-jasmone (cj) is synthesized from α-linolenic acid (la) via two biosynthetic pathways using jasmonic acid (ja) and iso-12-oxo-phytodienoic acid (iso-opda) as key intermediates. however, there have been no reports documenting cj production by microorganisms. in the present study, the production of fungal-derived cj by lasiodiplodia theobromae was observed for the first time, although this production was not observed for botrytis cinerea, verticillium longisporum, fusarium oxysporum, ...201728751737
fusarium keratitis in germany.fusarium keratitis is a destructive eye infection that is difficult to treat and which results in poor outcome. in the tropical and subtropical areas the infection is relatively common and associated with trauma or chronic eye diseases. however, in recent years, an increased incidence has been reported in temperate climate regions. at the german national reference center, we observed a steady increase in case numbers since 2014. here, we present the first german case series of eye infections wit ...201728747368
the distribution of miniature impala elements and six genes in the fusarium genus is suggestive of horizontal gene transfer.the mimp family of miniature inverted-repeat transposable elements was previously found only in genomes of fusarium oxysporum and is contextually associated with virulence genes in this species. through extensive comparative analysis of 83 f. oxysporum and 52 other fusarium genomes, we uncovered the distribution of different mimp families throughout the genus. we show that (i) mimps are not exclusive to f. oxysporum; (ii) pathogenic isolates generally possess more mimps than non-pathogenic strai ...201728744785
the distribution and host range of the banana fusarium wilt fungus, fusarium oxysporum f. sp. cubense, in asia.fusarium oxysporum formae specialis cubense (foc) is a soil-borne fungus that causes fusarium wilt, which is considered to be the most destructive disease of bananas. the fungus is believed to have evolved with its host in the indo-malayan region, and from there it was spread to other banana-growing areas with infected planting material. the diversity and distribution of foc in asia was investigated. a total of 594 f. oxysporum isolates collected in ten asian countries were identified by vegetat ...201728719631
production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source.the aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi (wrf) using peels or discarded potato as the sole nutrient source.201728718996
coupling the pretreatment and hydrolysis of lignocellulosic biomass by the expression of beta-xylosidases.thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. the improvement of lignocellulosic enzyme cocktails has been significant in the recent years. although the needs for the reduction of the energy intensity and chemical consumption in the pretreatment step are well known, the reduction of the severity of the process strongly affects the enzymatic hydrolysis yield. to explore t ...201728710860
comparative study on antifungal activities of chitosan nanoparticles and chitosan silver nano composites against fusarium oxysporum species complex.though the metal nanoparticles (nps) have been shown favorable results against fungal diseases, erratic environmental toxicity of nps have raised serious concerns against their applications. hence, it is vital to modify antifungal compounds into safe substitutes over synthetic chemicals. in this study, antifungal effects of chitosan nanoparticles (cnps) and chitosan silver nanocomposites (cagncs) were compared against fusarium oxysporum species complex. cnps and cagncs were synthesized, characte ...201728709896
endophytic fungi harbored in panax notoginseng: diversity and potential as biological control agents against host plant pathogens of root-rot disease.endophytic fungi play an important role in balancing the ecosystem and boosting host growth. in the present study, we investigated the endophytic fungal diversity of healthy panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of p. notoginseng.201728701877
modulation of inter-kingdom communication by phcbsr quorum sensing system in ralstonia solanacearum phylotype i strain gmi1000.ralstonia solanacearum is a ubiquitous soil-borne plant pathogenic bacterium, which frequently encounters and interacts with other soil cohabitants in competition for environmental niches. ralsolamycin, which is encoded by the rmy genes, has been characterized as a novel inter-kingdom interaction signal that induces chlamydospore development in fungi. in this study, we provide the first genetic evidence that the rmy gene expression is controlled by the phcbsr quorum sensing (qs) system in strain ...201728690607
role of fusaric acid in the development of 'fusarium wilt' symptoms in tomato: physiological, biochemical and proteomic perspectives.fusarium wilt is one of the most prevalent and damaging diseases of tomato. among various toxins secreted by the fusarium oxysporum f. sp. lycopersici (causal agent of fusarium wilt of tomato), fusaric acid (fa) is suspected to be a potent pathogenicity factor in tomato wilt disease development. with this rationale the present study was carried out with physiological, biochemical and proteomic perspectives. treatment of fa was given to the leaves of tomato directly through infiltration to show t ...201728683401
interdigital intertrigo due to fusarium oxysporum.fusariosis is a fungal infection often involving the skin. various species can cause local, focally invasive, or disseminated infections. the routes of entry for fusarium species include the respiratory tract, gastrointestinal tract, toe nails, trauma to the skin, and indwelling central venous catheter.201628681012
host-induced silencing of pathogenicity genes enhances resistance to fusarium oxysporum wilt in tomato.this study presents a novel approach of controlling vascular wilt in tomato by rnai expression directed to pathogenicity genes of fusarium oxysporum f. sp. lycopersici. vascular wilt of tomato caused by fusarium oxysporum f. sp. lycopersici leads to qualitative and quantitative loss of the crop. limitation in the existing control measures necessitates the development of alternative strategies to increase resistance in the plants against pathogens. recent findings paved way to rnai, as a promisin ...201728674943
isolation, identification, optimization, and metabolite profiling of streptomyces sparsus vsm-30.deep sea sediment samples of bay of bengal (visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. the actinomycetes strain vsm-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. it was identified as streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. response surface methodology regression analysis was carried out to fi ...201728669076
antifungal activity and expression patterns of extracellular chitinase and β-1,3-glucanase in wickerhamomyces anomalus eg2 treated with chitin and glucan.in this study, the expression patterns of extracellular chitinase and β-1,3-glucanase from cultured wickerhamomyces anomalus eg2 treated with chitin, glucan, and chemical chitinase inhibitors (kinetin, caffeine, and acetazolamide) were investigated using sds-page. relationship between enzyme expression and antifungal activity from yeast plays a very important role for biocontrol of phytopathoges. to determine antifungal activity against phytopathogens, w. anomalus eg2 was shown to strongly inhib ...201728668604
genetic diversity and antifungal susceptibility of fusarium isolates in onychomycosis.fusarium species have emerged as an important human pathogen in skin disease, onychomycosis, keratitis and invasive disease. onychomycosis caused by fusarium spp. the infection has been increasingly described in the immunocompetent and immunosuppressed hosts. considering onychomycosis is a difficult to treat infection, and little is known about the genetic variability and susceptibility pattern of fusarium spp., further studies are necessary to understand the pathogenesis and better to define th ...201728657120
novel loop-mediated isothermal amplification (lamp) assay with a universal qprobe can detect snps determining races in plant pathogenic fungi.tomato wilt pathogen fusarium oxysporum f. sp. lycopersici (fol) is grouped into three races based on their pathogenicity to different host cultivars. rapid detection and discrimination of fol races in field soils is important to prevent tomato wilt disease. although five types of point mutations in secreted in xylem 3 (six3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. the aim of this study is t ...201728652587
an evaluation method for suppression of pathogenic fusarium oxysporum by soil microorganisms using the dilution plate technique.volume 31, no. 3, page 307-313, 2016page 310, legend for fig. 4 incorrectfig. 4. growth degree of fusarium oxysporum f. sp. spinaciae for organic fertilizers at each dilution based on an estimation of the ellipse area (a) and extension length (b) of the colony. cont, compound inorganic fertilizer; sbm, steamed bone meal; cdc, cow dung compost; mi, microbial inoculant. values show mean of medians of degrees with se (n=3). the ellipse area and extension length for control plates were 4,475 mm(2) a ...201728652549
a meta-analysis of quantitative trait loci for abiotic and biotic stress resistance in tetraploid cotton.the number and location of mapped quantitative trait loci (qtl) depend on genetic populations and testing environments. the identification of consistent qtl across genetic backgrounds and environments is a pre-requisite to marker-assisted selection. this study analyzed a total of 661 abiotic and biotic stress resistance qtl based on our previous work and other publications using the meta-analysis software biomercator. it identified chromosomal regions containing qtl clusters for different resist ...201728647758
isolation of a potential biocontrol agent paenibacillus polymyxa nsy50 from vinegar waste compost and its induction of host defense responses against fusarium wilt of cucumber.fusarium wilt caused by fusarium oxysporum f. sp. cucumerinum (foc) is one of the major destructive soil-borne diseases infecting cucumber. in this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. the 16s rdna gene demonstrated that the biocontrol agents were paenibacillus polymyxa (p. polymyxa), bacillus amyloliquefaciens (b. amyloliquefaciens) and bacillus ...201728647117
a novel photosensitization treatment for the inactivation of fungal spores and cells mediated by curcumin.the global concerns regarding the emergence of fungicide-resistant strains and the impact of the excessive use of fungicidal practises on our health, food, and environment have increased, leading to a demand for alternative clean green technologies as treatments. photosensitization is a treatment that utilises a photosensitiser, light and oxygen to cause cell damage to microorganisms. the effect of photosensitization mediated by curcumin on aspergillus niger, aspergillus flavus, penicillium gris ...201728623822
identification of a potential isr determinant from pseudomonas aeruginosa pm12 against fusarium wilt in tomato.biocontrol of plant diseases through induction of systemic resistance is an environmental friendly substitute to chemicals in crop protection measures. different biotic and abiotic elicitors can trigger the plant for induced resistance. present study was designed to explore the potential of pseudomonas aeruginosa pm12 in inducing systemic resistance in tomato against fusarium wilt. initially the bioactive compound, responsible for isr, was separated and identified from extracellular filtrate of ...201728620396
chemical composition and antifungal activity of hedera helix leaf ethanolic extract.the 50% ethanol extract obtained from hedera helix leaves was investigated regarding the presence and quantity of polyphenols, sterols and in vitro antifungal activity against phytopathogenic fungi. the chemical analysis revealed the presence of rutin, quercetin and kaempferol in the non-hydrolysed sample and quercetin and kaempferol in the hydrolysed sample and stigmasterol in the ivy leaf extract (nonhydrolysed sample). the antifungal activity against phytopathogenic fungi (aspergillus niger, ...201728605982
different mechanisms of trichoderma virens-mediated resistance in tomato against fusarium wilt involve the jasmonic and salicylic acid pathways.in the present study, we investigated the role of trichoderma virens (triv_jsb100) spores or cell-free culture filtrate in the regulation of growth and activation of the defence responses of tomato (solanum lycopersicum) plants against fusarium oxysporum f. sp. lycopersici by the development of a biocontrol-plant-pathogen interaction system. two-week-old tomato seedlings primed with triv_jsb100 spores cultured on barley grains (bgs) or with cell-free culture filtrate (cf) were inoculated with fu ...201728605157
the arabidopsis leucine-rich repeat receptor kinase mik2/lrr-kiss connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses.plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. however, few components involved in plant cell wall integrity sensing have been described to date. using a reverse-genetic approach, we identified the arabidopsis thaliana leucine-rich repeat receptor kinase mik2 as an important regulator of cell wall damage responses triggered upon cellulose biosynthesis inhibition. indeed, loss-of-function mik2 alleles are strongly ...201728604776
fusarium infection causes genotoxic disorders and antioxidant-based damages in orobanche spp.this study aims to evaluate the toxic effects of fusarium oxysporum on root parasitic weed, orobanche spp. comparative genetic and gene expression studies were conducted on uninfected and fungus-infected orobanches. in genetic studies, isolated total dna was amplified by rapd pcr. fragment properties were analysed by gts test. according to the results, the fragment properties of control and fusarium infected (experimental) groups varied widely; and it has been observed that fusarium has genotoxi ...201728602401
in vitro and in vivo antifungal efficacy of plant based lawsone against fusarium oxysporum species complex.fusarium oxysporum is an ascomycete facultative fungus which generally affects to plants. however, it is recently known as a serious emerging opportunistic pathogen of human and other animals. f. oxysporum shows broad resistance to commonly used antifungal agents and therefore development of alternative therapeutic agents is required. in this study, we investigated the antifungal efficacy of plant based natural lawsone against pathogenic f. oxysporum. antifungal susceptibility test determined th ...201728602398
purification, characterization and retting of crotolaria juncea fibres by an alkaline pectin lyase from fusarium oxysporum mtcc 1755.using solid-state fermentation, production of an industrially important pectin lyase from a fungal strain fusarium oxysporum mtcc 1755 was attempted, which was further subjected to purification and characterization. the enzyme was purified by three steps, namely ammonium sulfate fractionation, cation-exchange chromatography on cm cellulose followed by gel filtration chromatography using sephadex g-100 column. a 16-fold purification with 31.2% yield and 3.2 u/mg specific activity was achieved. th ...201728593518
polyketide-terpene hybrid metabolites from an endolichenic fungus pestalotiopsis sp.five new polyketide-terpene hybrid metabolites (1-5) with highly functionalized groups, together with six known derivatives (6-11), were isolated from the endolichenic fungus pestalotiopsis sp. their structures were elucidated by extensive nmr experiments including (1)h, (13)c, hmqc, cosy, and hmbc. the relative configurations of the new compounds were determined by analysis of coupling constants and roesy correlations. the absolute configurations especially the secondary alcohol at c-15 in 1 an ...201728593175
evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms.scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus).201728587870
specific pcr detection of fusarium oxysporum f. sp. raphani: a causal agent of fusarium wilt on radish plants.fusarium oxysporum, a causal agent of fusarium wilt, is one of the most important fungal pathogens worldwide, and detection of f. oxysporum dna at the forma specialis level is crucial for disease diagnosis and control. in this study, two novel f. oxysporum f. sp. raphani (for)-specific primer sets were designed, for1-f/for1-r and for2-f/for2-r, to target foqg_17868 and foqg_17869 orfs, respectively, which were selected based on the genome comparison of other formae speciales of f. oxysporum incl ...201728585248
biodiversity in targeted metabolomics analysis of filamentous fungal pathogens by (1)h nmr-based studies.the taxonomical classification among fungi kingdom in the last decades was evolved. in this work the targeted metabolomics study based on (1)h nmr spectroscopy combined with chemometrics tools was reported to be useful for differentiation of three model of fungal strains, which represent various genus of ascomycota (aspergillus pallidofulvus, fusarium oxysporum, geotrichum candidum) were selected in order to perform metabolomics studies. each tested species, revealed specific metabolic profile o ...201728585165
application of surface modified nano ferrite nickel in catalytic reaction (epoxidation of alkenes) and investigation on its antibacterial and antifungal activities.newly, magnetic nanoparticles have extensively been used as alternative catalyst supports, in the view of their high surface area which results in high catalyst loading capacity, high dispersion, low toxicity, environmental preservation, distinguished stability, and suitable catalyst reusing. in the present study, the magnetite nanoparticles, nife2o4@ag and nife2o4@mo, were synthesized and characterized. the antimicrobial activities and catalytic properties of synthesized nanoparticles were test ...201728575933
corrigendum: transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.[this corrects the article on p. 362 in vol. 8, pmid: 28367157.].201728572815
an allene oxide and 12-oxophytodienoic acid are key intermediates in jasmonic acid biosynthesis by fusarium oxysporum.fungi can produce jasmonic acid (ja) and its isoleucine conjugate in large quantities, but little is known about the biosynthesis. plants form ja from 18:3n-3 by 13s-lipoxygenase (lox), allene oxide synthase, and allene oxide cyclase. shaking cultures of fusarium oxysporum f. sp. tulipae released over 200 mg of jasmonates per liter. nitrogen powder of the mycelia expressed 10r-dioxygenase-epoxy alcohol synthase activities, which was confirmed by comparison with the recombinant enzyme. the 13s-lo ...201728572515
tobacco rattle virus-based silencing of enoyl-coa reductase gene and its role in resistance against cotton wilt disease.a tobacco rattle virus (trv)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (gossypium hirsutum). this approach was used to investigate the function of the enoyl-coa reductase (ghecr) gene in pathogen defense. amino acid sequence alignment of arabidopsis ecr with homologous sequence from g. hirsutum, g. arboreum, g. herbaceum and g. barbadense showed that ecrs are highly conserved among these species. trv-based silencing of gh ...201728567686
illumina-based sequencing analysis directed selection for actinobacterial probiotic candidates for banana plants.as potential probiotic candidates, plant vertically transmitted actinobacteria are beneficial to growth and health of host plants. new methods to isolate the actinobacterial taxa with low growth rates should be developed. based on the actinobacterial population information, the probiotic actinobacterial taxa could be directly isolated from healthy banana shoot tips. however, actinobacterial dnas with high gc contents could bias estimates of actinobacteria by pcr. in the study, two amplicon seque ...201728560514
small rna sequencing for secondary metabolite analysis in persicaria minor.persicaria minor (kesum) is an important medicinal plant and commonly found in southeast countries; malaysia, thailand, indonesia, and vietnam. this plant is enriched with a variety of secondary metabolites (sms), and among these sms, terpenoids are in high abundance. terpenoids are comprised of many valuable biomolecules which have well-established role in agriculture and pharmaceutical industry. in p. minor, for the first time, we have generated small rnas data sets, which can be used as tool ...201728560169
production, characterization, and antifungal activity of a biosurfactant produced by rhodotorula babjevae ys3.sophorolipids are one of the most promising glycolipid biosurfactants and have been successfully employed in bioremediation and various other industrial sectors. they have also been described to exhibit antimicrobial activity against different bacterial species. nevertheless, previous literature pertaining to the antifungal activity of sophorolipids are limited indicating the need for further research to explore novel strains with wide antimicrobial activity. a novel yeast strain, rhodotorula ba ...201728558761
hairy root transgene expression analysis of a secretory peroxidase (pvpox1) from common bean infected by fusarium wilt.plant peroxidases (poxs) are one of the most important redox enzymes in the defense responses. however, the large number of different plant pox genes makes it necessary to carefully confirm the function of each paralogous pox gene in specific tissues and disease interactions. fusarium wilt is a devastating disease of common bean caused by fusarium oxysporum f. sp. phaseoli. in this study, we evaluated a peroxidase gene, pvpox1, from a resistant common bean genotype, caas260205 and provided direc ...201728554466
a novel α-galactosidase from fusarium oxysporum and its application in determining the structure of the gum arabic side chain.we previously reported that fusarium oxysporum 12s produces two bifunctional proteins, foap1 and foap2, with α-d-galactopyranosidase (gpase) and β-l-arabinopyranosidase (apase) activities. the aim of this paper was to purify a third gpase, fogp1, from culture supernatant of f. oxysporum 12s, to characterize it, and to determine its mode of action towards gum arabic. a cdna encoding fogp1 was cloned and the protein was overexpressed in escherichia coli. module sequence analysis revealed the prese ...201728554382
clean up fly ash from coal burning plants by new isolated fungi fusarium oxysporum and penicillium glabrum.in turkey approximately 45 million tons of coals are burned in a year and 19.3 million tons of fly ash have emerged. the bioremediation of heavy metals or different elements from fly ash makes them bio-available. however, in previous studies, requiring of long operational time and failing to show tolerance to high pulp densities of fly ash of selected fungal species makes them impractical. in this work, bioremediation of fly ash by new isolated fungi fusarium oxysporum and penicillium glabrum we ...201728554126
analysis of protein profile of tomato root infected with fusarium oxysporum f. sp lycopersici.fusarium wilt caused by fusarium oxysporum f. sp lycopersici (fol) is one of the main diseases affecting tomatoes. the bhrs 2,3 genotype of tomato is, however, resistant to this disease. a proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. protein was extracted and separated by two-dimensional electrophoresis followed by staining with coomassie brilliant blue. the proteins were ide ...201728549197
transcriptomic dissection reveals wide spread differential expression in chickpea during early time points of fusarium oxysporum f. sp. ciceri race 1 attack.plants' reaction to underground microorganisms is complex as sessile nature of plants compels them to prioritize their responses to diverse microorganisms both pathogenic and symbiotic. roots of important crops are directly exposed to diverse microorganisms, but investigations involving root pathogens are significantly less. thus, more studies involving root pathogens and their target crops are necessitated to enrich the understanding of underground interactions. present study reported the molec ...201728542579
characterization of silver nanoparticles produced by biosynthesis mediated by fusarium oxysporum under different processing conditions.the goal of this study was the biosynthesis of silver nanoparticles (snps) mediated by the fungus fusarium oxysporum, as well as the characterization of these nanoparticles including evaluation of the particles size and stability under different processing conditions. the results showed that the biosynthesis produced silver nanoparticles having a mean size of 34 nm and zeta potential values below -30 mv at the conditions used, characterizing the nanoparticles as being stable in suspension. ultra ...201728540439
detoxification of fusaric acid by the soil microbe mucor rouxii.fusarium oxysporum f. sp. vasinfectum race 4 (vcg0114), which causes root rot and wilt of cotton (gossypium hirsutum and g. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the san joaquin valley of california. this pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of f. oxysporum f. sp. vasinfectum (fov) that are indigenous to the united states. fusaric a ...201728538103
lipopeptides produced by bacillus subtilis as new biocontrol products against fusariosis in ornamental plants.in this study, we have investigated the effects of three lipopeptides (fengycin, surfactin and mycosubtilin) produced by different strains of bacillus subtilis against the phytopathogenic fungi fusarium oxysporum f. sp. iridacearum, which affects the ornamental bulb plant populations of iris sp. the antifungal effects were tested using minimum inhibitory concentration assay, determination of mycelium growth and spore germination inhibition rates. also, in vivo tests on infected rhizomes and scan ...201728528498
wble2 transcription factor in streptomyces griseus s4-7 plays an important role in plant protection.streptomyces griseus s4-7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for fusarium wilt suppressive soils. s. griseus s4-7 shows specific and pronounced antifungal activity against fusarium oxysporum f. sp. fragariae. in the streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wble2 function as a regulator has emerged as a new group in whi transcription ...201728523731
non-edible oil cakes as a novel substrate for dpa production and augmenting biocontrol activity of paecilomyces variotii.the present study investigated the use of waste non-edible oil cakes (jatropha, karanja, neem, and mahua) as a substrate for the growth of paecilomyces variotii and dipicolinic acid (dpa) production. previous researches proved the efficacy of dpa in suppressing certain pathogens that are deleterious to the plants in the rhizosphere. dpa production was statistical optimized by amending non-edible oil cakes in growing media as nitrogen and sugars (dextrose, glucose, and lactose) as carbon source. ...201728512455
purification and site-directed mutagenesis of linoleate 9s-dioxygenase-allene oxide synthase of fusarium oxysporum confirms the oxygenation mechanism.plants and fungi form jasmonic acid from α-linolenic acid. the first two steps of biosynthesis in plants occur by sequential transformation by 13s-lipoxygenase and allene oxide synthase (aos). the biosynthesis in fungi may follow this classical scheme, but the only fungal aos discovered so far are cytochromes p450 (cyp) fused to 8- and 9-dioxygenases (dox). in the present report, we purified recombinant 9s-dox-aos of fusarium oxysporum from cell lysate by cobalt affinity chromatography to near h ...201728502466
effectiveness of a full-scale horizontal slow sand filter for controlling phytopathogens in recirculating hydroponics: from microbial isolation to full microbiome assessment.the microbial disinfestation efficiency of an innovative horizontal-flow slow sand filter (hssf) for treating nutrient solution spent from an experimental closed-loop nursery was evaluated by means of a combination of culture-dependent and independent molecular techniques. a dense inoculum of the fungal plant pathogen fusarium oxysporum f.sp. lycopersici was applied in the fertigation system (10(6) cells per ml). indigenous and introduced populations of eubacteria and fungi were assessed in the ...201728499226
isolated bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing pcr.plant growth-promoting bacteria (pgpb) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. in this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. based on 16s rdna and biolog test analysis, the 330-2 strain was identified as bacillus subtilis. the strain produced indole-3-acetic acid, siderophores, lytic e ...201728496135
fungal endophytes isolated from protium heptaphyllum and trattinnickia rhoifolia as antagonists of fusarium oxysporum.control of fungal pathogens is mainly addressed by the use of chemically synthesized fungicides which result in environmental pollution, developing resistance after prolonged use. in this context, endophytes have been recognized as potential biocontrollers, and also as a promising source of antifungal metabolites. therefore, as part of our research on phytopathogen controllers, 355 fungal endophytes were isolated from protium heptaphyllum and trattinnickia rhoifolia (burseraceae), both ethnobota ...201728495036
fusarium oxysporum and the fusarium wilt syndrome.the fusarium oxysporum species complex (fosc) comprises a multitude of strains that cause vascular wilt diseases of economically important crops throughout the world. although sexual reproduction is unknown in the fosc, horizontal gene transfer may contribute to the observed diversity in pathogenic strains. development of disease in a susceptible crop requires f. oxysporum to advance through a series of transitions, beginning with spore germination and culminating with establishment of a systemi ...201728489498
the endochitinase vdech from verticillium dahliae inhibits spore germination and activates plant defense responses.chitinases function in the digestion of chitin molecules, which are present principally in insects and fungi. in plants, chitinase genes play important roles in defense, and their expression can be triggered in response to both biotic and abiotic stresses. in this study, we cloned and characterized an endochitinase (vdech) from verticillium dahliae, strain vd080. the vdech coding region consists of 1845bp with two exons and one 54bp intron, encoding a 615 amino acid protein with the predicted mo ...201728483050
in vitro antifungal, probiotic, and antioxidant functional properties of a novel lactobacillus paraplantarum isolated from fermented dates in saudi arabia.fermented foods produced using dates are used in gulf countries as beneficial and healthful foods. the beneficial microbial flora in fermented dates contributes to maintaining the nutritional properties of dates by preventing the growth of spoilage fungi. here, we examined the antifungal, probiotic, and antioxidant properties of the novel lactobacillus strain d-3 isolated from fermented dates.201728480570
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds.natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars.201728480436
plipastatin and surfactin coproduction by bacillus subtilis pb2-l and their effects on microorganisms.to convert the lipopeptide non-producer strain bacillus subtilis pb2 into a plipastatin and surfactin coproducer, a gene expression cassette composed of a constitutive promoter (p43), functional gene sfp, and pleiotropic regulatory gene degq was integrated into the chromosomal amye locus of strain b. subtilis pb2 by homologous recombination, which generated a plipastatin and surfactin co-producer. thirteen plipastatins and fifteen surfactins were identified in lipopeptide extracts using analytic ...201728477175
comparative transcriptomic analysis of race 1 and race 4 of fusarium oxysporum f. sp. cubense induced with different carbon sources.the fungal pathogen fusarium oxysporum f. sp. cubense causes fusarium wilt, one of the most destructive diseases in banana and plantain cultivars. pathogenic race 1 attacks the "gros michel" banana cultivar, and race 4 is pathogenic to the cavendish banana cultivar and those cultivars that are susceptible to foc1. to understand the divergence in gene expression modules between the two races during degradation of the host cell wall, we performed rna sequencing to compare the genome-wide transcrip ...201728468818
genome-wide analysis of autophagy-related genes in banana highlights maatg8s in cell death and autophagy in immune response to fusarium wilt.maatg8s play important roles in hypersensitive-like cell death and immune response, and autophagy is essential for disease resistance against foc in banana. autophagy is responsible for the degradation of damaged cytoplasmic constituents in the lysosomes or vacuoles. although the effects of autophagy have been extensively revealed in model plants, the possible roles of autophagy-related gene in banana remain unknown. in this study, 32 maatgs were identified in the draft genome, and the profiles ...201728451821
design, synthesis of novel chitosan derivatives bearing quaternary phosphonium salts and evaluation of antifungal activity.two novel chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized, including tricyclohexylphosphonium acetyl chitosan chloride (tcpacsc) and triphenylphosphonium acetyl chitosan chloride (tppacsc), and characterized by ftir, (1)h nmr, and (13)c nmr spectra. the degree of substitution was also calculated by elemental analysis results. their antifungal activities against colletotrichum lagenarium, watermelon fusarium, and fusarium oxysporum were investigated i ...201728445700
mediator18 and mediator20 confer susceptibility to fusarium oxysporum in arabidopsis thaliana.the conserved protein complex known as mediator conveys transcriptional signals by acting as an intermediary between transcription factors and rna polymerase ii. as a result, mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. in this report we identify the head domain subunits mediator18 and mediator20 as important susceptibility factors for fusarium oxysporum infection in arabidopsis thaliana. mutants of med18 and med20 display down- ...201728441405
the antibiotic polymyxin b exhibits novel antifungal activity against fusarium species.the genus fusarium comprises many species, including fusarium oxysporum, fusarium solani, fusarium graminearum and fusarium verticillioides, and causes severe infections in plants and humans. in clinical settings, fusarium is the third most frequent mould to cause invasive fungal infections after aspergillus and the mucorales. f. solani and f. oxysporum are the most prevalent fusarium spp. causing clinical disease. however, few effective antifungal drugs are available to treat human and plant fu ...201728433743
essential oils as biocides for the control of fungal infections and devastating pest (tuta absoluta) of tomato (lycopersicon esculentum mill.).thymus capitatus and tetraclinis articulata essential oils as well their major components (carvacrol and α-pinene) were evaluated for their antifungal and insecticidal activities. both oils showed good in vitro antifungal activity against fusarium oxysporum, aspergillus niger, penicillium sp., alternaria alternata, and botrytis cinerea, the fungi causing tomato rot. in vivo results indicate the efficacies of both essential oils and carvacrol of reduce postharvest fungal pathogens, such as b. cin ...201728422413
antimicrobial textiles: biogenic silver nanoparticles against candida and xanthomonas.this paper introduces cotton fibers impregnated with biogenic silver nanoparticles (agnps), synthesized from a fusarium oxysporum fungal filtrate (ff) solution, and open up the possibility for their use in medical environment and agriculture clothing as means to avoid microbial spreading. after thorough agnps characterization, regarding their physical, chemical and biochemical properties, minimum inhibitory concentrations (mic) against some human and orange tree pathogens were determined. we rep ...201728415502
infection courts in watermelon plants leading to seed infestation by fusarium oxysporum f. sp. niveum.fusarium wilt incited by fusarium oxysporum f. sp. niveum (fon) is a seed-transmitted disease that causes significant yield loss in watermelon production. the pathogen may infect watermelon seeds latently, which can be an important inoculum source and contribute to severe disease outbreak. however, information regarding infection courts of fon leading to infestation of watermelon seeds is limited. to determine how seeds in watermelon fruit can be infested by fon during the watermelon growing sea ...201728414630
nutritional composition and phytochemical, antioxidative, and antifungal activities of pergularia tomentosa l.crude extracts from a medicinal tunisian plant, pergularia tomentosa l., were the investigated natural material. butanolic extract of roots analyzed with ir spectra revealed the presence of hydroxyl, alcoholic, and carboxylic groups and sugars units. analysis of some secondary metabolites, total phenolic, flavonoids, flavonols, and procyanidins, was performed using different solvents following the increased gradient of polarity. fruits and leaves contained the highest amounts of all these compou ...201728409159
immobilization of a cutinase from fusarium oxysporum and application in pineapple flavor synthesis.in the present study, the immobilization of a cutinase from fusarium oxysporum was carried out as cross-linked enzyme aggregates. under optimal immobilization conditions, acetonitrile was selected as precipitant, utilizing 9.4 mg protein/ml and 10 mm glutaraldehyde as cross-linker. the immobilized cutinase (imfocut5a) was tested in isooctane for the synthesis of short-chain butyrate esters, displaying enhanced thermostability compared to the free enzyme. pineapple flavor (butyl butyrate) synthes ...201728403608
endophytic fungi associated with monarda citriodora, an aromatic and medicinal plant and their biocontrol potential.the food and agriculture organization has estimated that every year considerable losses of the food crops occur due to plant diseases. although fungicides are extensively used for management of plant diseases, they are expensive and hazardous to the environment and human health. alternatively, biological control is the safe way to overcome the effects of plant diseases and to sustain agriculture. since monarda citriodora cerv. ex lag. (lamiaceae/labiatae) is known for its antifungal properties, ...201728398103
methanolic extract of plumbago zeylanica - a remarkable antibacterial agent against many human and agricultural pathogens.the current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of plumbago zeylanica.201728392958
involvement of salicylic acid, ethylene and jasmonate acid signaling pathways in susceptibility of tomato for fusarium oxysporum.phytohormones such as salicylic acid (sa), ethylene (et) and jasmonic acid (ja) play key roles in plant defense following pathogen attack. involvement of these hormones in susceptibility following fusarium oxysporum (fo) infection has mostly been studied in arabidopsis thaliana. fo, however, causes vascular wilt disease in a broad range of crops, including tomato (solanum lycopersicum). surprisingly little is known about the involvement of these phytohormones in susceptibility of tomato towards ...201728390170
rapd assisted selection of black gram (vigna mungo l. hepper) towards the development of multiple disease resistant germplasm.black gram (vigna mungo l. hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. the yield potential of black gram is shallow due to lack of genetic variability and biotic stress susceptibility. core biotic stress factors include mung bean yellow mosaic virus (mymv), urdbean leaf crinkle virus (uclv), wilt (fusarium oxysporum) and powdery mildew (erysiphe polygoni dc). although many studies determine resistant varieties to a parti ...201728389895
investigation of genetic diversity of fusarium oxysporum f. sp. fragariae using pcr-rflp.fusarium wilts of strawberry, caused by fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. reliable chemical control of strawberry fusarium wilt disease is not yet available. moreover, other well-known f. oxysporum have different genetic information from f. oxysporum f. sp. fragariae. this analysis investigates the genetic diversity of strawberry fusairu ...201728381961
seed vectored endophytic bacteria modulate development of rice seedlings.the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens.201728375579
prospecting fungal parasites of the potato cyst nematode globodera pallida using a rapid screening technique.seven filamentous fungal species were isolated from individual eggs of globodera pallida cysts collected from infested fields in shelley idaho, usa and identified as chaetomium globosum, fusarium oxysporum, fusarium solani, fusarium tricinctum, microdochium bolleyi, purpureocillium lilacinum, and plectosphaerella cucumerina. their ability to reduce infection by g. pallida in planta were assessed in simple, reproducible micro-rhizosphere chambers (micro-rocs). all fungi reduced g. pallida infecti ...201728375550
fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems.the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ...201728369915
transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.fusarium oxysporum f. sp. melonis snyd. & hans race 1.2 (fom1.2) is the most virulent and yield-limiting pathogen of melon (cucumis melo l.) worldwide. current information suggest that the resistance to race 1.2 is controlled by multiple recessive genes and strongly affected by the environment. rna-seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes deployed during melon-fom1.2 interaction in the resistant doubled haploid line nad and in the ...201728367157
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