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in vitro antifungal activity of synthetic dsrna molecules against two pathogens of banana, fusarium oxysporum f. sp. cubense and mycosphaerella fijiensis.a key challenge for designing rnai-based crop protection strategies is the identification of effective target genes in the pathogenic organism. in this study, in vitro antifungal activities of a set of synthetic double-stranded rna molecules on spore germination of two major pathogenic fungi of banana, fusarium oxysporum schlecht f. sp. cubense wc snyder & hn hans (foc) and mycosphaerella fijiensis morelet (mf) were evaluated.201323471899
a calcium ion-dependent dimeric bean lectin with antiproliferative activity toward human breast cancer mcf-7 cells.in this study, a 60.8-kda dimeric lectin was isolated from the phaseolus vulgaris cv. jade bean and characterized. the lectin was bound on blue sepharose 6 and q sepharose and was finally purified by size exclusion chromatography on superdex 200. its hemagglutinating activity toward rabbit erythrocytes was dependent on divalent cations, especially calcium ions. various carbohydrates tested were devoid of any effect on the hemagglutinating activity. the lectin was stable at ph between 4.5 and 9.4 ...201323475505
volatile substances produced by fusarium oxysporum from coffee rhizosphere and other microbes affect meloidogyne incognita and arthrobotrys conoides.microorganisms produce volatile organic compounds (vocs) which mediate interactions with other organisms and may be the basis for the development of new methods to control plant-parasitic nematodes that damage coffee plants. in the present work, 35 fungal isolates were isolated from coffee plant rhizosphere, meloidogyne exigua eggs and egg masses. most of the fungal isolates belonged to the genus fusarium and presented in vitro antagonism classified as mutual exclusion and parasitism against the ...201223482720
toll-like receptors and myd88 adaptors in mytilus: complete cds and gene expression levels.tlr- and myd88-related sequences have been previously investigated in mytibase and then in new transcript reads obtained by illumina technology from the mussel, mytilus galloprovincialis. based on full cds and domain organizations of virtual translations, we identified 23 toll-like receptors (tlrs) and 3 myd88 adaptors. mgtlrs can be arranged in 4 clusters according to extra-cellular lrr domain content. mgtlr-b, -i and -k were the only ones containing a multiple cysteine cluster (mcctlr), a doma ...201323485525
comparative proteome analysis of the strawberry-fusarium oxysporum f. sp. fragariae pathosystem reveals early activation of defense responses as a crucial determinant of host resistance.fusarium wilt on strawberry caused by fusarium oxysporum f. sp. fragariae (fof) is a serious threat to commercial strawberry production worldwide. however, resistance mechanisms of strawberry against fof remain unknown. to reveal the defense responses of strawberry against fof, comparative proteome analyses were conducted to determine temporal changes in root proteomes of the resistant cv. festival and susceptible cv. camarosa from 4 to 72 h post inoculation with fof. analysis of proteins separa ...201323495785
an antifungal peptide from coffea canephora seeds with sequence homology to glycine-rich proteins exerts membrane permeabilization and nuclear localization in fungi.the superfamily of glycine-rich proteins (grps) corresponds to a large and complex group of plant proteins that may be involved in many developmental and physiological processes such as rna biogenesis, stress tolerance, pollen hydration and plant-pathogen interactions, showing defensive activity against fungi, bacteria and viruses.201323500079
synthesis and structure-activity relationship of n-(cinnamyl) chitosan analogs as antimicrobial agents.the current study focuses on the preparation of new n-(cinnamyl) chitosan derivatives as antimicrobial agents against nine types of crop-threatening pathogens. chitosan was reacted with a set of aromatic cinnamaldehyde analogs by reductive amination involving formation of the corresponding imines, followed by reduction with sodium borohydride to produce n-(cinnamyl) chitosan derivatives. the structural characterization was confirmed by (1)h and (13)c nmr spectroscopy and the degrees of substitut ...201323511055
chemical composition and antifungal activity of matricaria recutita flower essential oil against medically important dermatophytes and soil-borne pathogens.fungal infections are potential public health threats all over the world. in the present study, effect of matricaria recutita flower essential oil (eo) was evaluated against medically important dermatophytes and opportunistic saprophytes using microbioassay technique.201223518164
signaling specificity provided by the arabidopsis thaliana heterotrimeric g-protein γ subunits agg1 and agg2 is partially but not exclusively provided through transcriptional regulation.the heterotrimeric g-protein complex in arabidopsis thaliana consists of one α, one ß and three γ subunits. while two of the γ subunits, agg1 and agg2 have been shown to provide functional selectivity to the gßγ dimer in arabidopsis, it is unclear if such selectivity is embedded in their molecular structures or conferred by the different expression patterns observed in both subunits. in order to study the molecular basis for such selectivity we tested genetic complementation of agg1- and agg2 dr ...201323520518
biological interactions between soil saprotrophic fungi and ascaris suum eggs.the in vitro effect of saprotrophic soil fungi on the embryonic development of ascaris suum was evaluated. the fungi tested were aspergillus fumigatus, aspergillus terreus, penicillium citrinum, penicillium expansum, fusarium oxysporum and trichothecium roseum, isolated from children's recreation areas in the city of lodz (poland). each species was co-cultured with a. suum egg suspension (6 × 10(3)eggs/ml) at 25 ± 2°c for 60 days. each day, 100 eggs were randomly collected and their developmenta ...201323534981
functional analysis of the cars gene of fusarium fujikuroi.the ascomycete fungus fusarium fujikuroi is a model system in the investigation of the biosynthesis of some secondary metabolites, such as gibberellins, bikaverin, and carotenoids. carotenoid-overproducing mutants, generically called cars, are easily obtained in this fungus by standard mutagenesis procedures. here we report the functional characterization of gene cars, responsible for this mutant phenotype. the identity of the gene was demonstrated through the finding of mutations in six indepen ...201323543145
the intercropping partner affects arbuscular mycorrhizal fungi and fusarium oxysporum f. sp. lycopersici interactions in tomato.arbuscular mycorrhizal fungi (amf) and their bioprotective aspects are of great interest in the context of sustainable agriculture. combining the benefits of amf with the utilisation of plant species diversity shows great promise for the management of plant diseases in environmentally compatible agriculture. in the present study, amf were tested against fusarium oxysporum f. sp. lycopersici with tomato intercropped with either leek, cucumber, basil, fennel or tomato itself. arbuscular mycorrhiza ...201323549903
bacillus amyloliquefaciens q-426 as a potential biocontrol agent against fusarium oxysporum f. sp. spinaciae.in recent years, bacillus species have received considerable attention for the biological control of many fungal diseases. in this study, bacillus amyloliquefaciens q-426 was tested for its potential use against a variety of plant pathogens. our screen for genes involved in the biosynthesis of antifungal agents revealed that the fen and bmy gene clusters are present in the q-426 genome. lipopeptides such as bacillomycin d, fengycin a, and fengycin b were purified from the bacterial culture broth ...201423553741
synthesis and antifungal activities of novel nicotinamide derivatives containing 1,3,4-oxadiazole.pant diseases in agriculture are extremely difficult to control, which bring about for billions of dollars in economic losses worldwide each year; nicotinamide derivatives has attracted more and more attention in the field of pesticide due to their broad bioactivities. in an effort to discover new molecules with good antifungal activity, a series of nicotinamide derivatives containing 1,3,4-oxadiazole were synthesized and bio-assayed.201323561596
characterization and expression analysis of dirigent family genes related to stresses in brassica.the dirigent (dir) genes are playing a vital role in enhancing stress resistance in different crop plants. in this study, we collected 29 dir like genes, two from a brassica rapa cv. osome full length cdna library and 27 from the b. rapa database designated as b. rapa dirigent (brdir) like genes. sequence analysis and a comparison study of these genes confirmed that seven were dirigent and the remaining 22 were dirigent like genes. expression analysis revealed an organ specific expression of the ...201323562798
isolation and characterization of a proteinaceous antifungal compound from lactobacillus plantarum yml007 and its application as a food preservative.korean kimchi is known for its myriad of lactic acid bacteria (lab) with diverse bioactive compounds. this study was undertaken to isolate an efficient antifungal lab strain among the isolated kimchi labs. one thousand and four hundred labs isolated from different kimchi samples were initially screened against aspergillus niger. the strain exhibiting the highest antifungal activity was identified as lactobacillus plantarum yml007 by 16s rrna sequencing and biochemical assays using api 50 chl kit ...201323565693
biotic and abiotic stress tolerance in transgenic tomatoes by constitutive expression of s-adenosylmethionine decarboxylase gene.recent findings have implicated the role of polyamines (putrescine, spermidine and spermine) in stress tolerance. therefore, the present work was carried out with the goal of generating transgenic tomato plants with human s-adenosylmethionine decarboxylase (samdc) gene, a key gene involved in biosynthesis of polyamines, viz. spermidine and spermine and evaluating the transgenic plants for tolerance to both biotic and abiotic stresses. several putative transgenic tomato plants with normal phenoty ...201123573001
wound-induced pectin methylesterases enhance banana (musa spp. aaa) susceptibility to fusarium oxysporum f. sp. cubense.recent studies suggest that plant pectin methylesterases (pmes) are directly involved in plant defence besides their roles in plant development. however, the molecular mechanisms of pme action on pectins are not well understood. in order to understand how pmes modify pectins during banana (musa spp.)-fusarium interaction, the expression and enzyme activities of pmes in two banana cultivars, highly resistant or susceptible to fusarium, were compared with each other. furthermore, the spatial distr ...201323580752
a novel and highly efficient method for genetic transformation of fungi employing shock waves.genetic transformation of filamentous fungi is an essential tool in many areas such as biotechnology, medicine, phytopathology and genetics. however, available protocols to transform fungi are inefficient, laborious and have low reproducibility. we report the use of underwater shock waves as a novel method to transform filamentous fungi. an experimental piezoelectric shock wave generator was designed to expose fungal conidia to heterologous dna. the device was successfully tested in aspergillus ...201323583899
a nuclear localization for avr2 from fusarium oxysporum is required to activate the tomato resistance protein i-2.plant pathogens secrete effector proteins to promote host colonization. during infection of tomato xylem vessels, fusarium oxysporum f. sp. lycopersici (fol) secretes the avr2 effector protein. besides being a virulence factor, avr2 is recognized intracellularly by the tomato i-2 resistance protein, resulting in the induction of host defenses. here, we show that avr2 is highly expressed in root- and xylem-colonizing hyphae three days post inoculation of roots. co-expression of i-2 with avr2 dele ...201323596453
evaluation of certain food additives.this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, including flavouring agents, with a view to concluding as to safety concerns and to preparing specifications for identity and purity. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of and assessment of dietary exposure to food additives, including flavouring agents. a summary follows of the commi ...201223600165
antimicrobial activity of pleurocidin is retained in plc-2, a c-terminal 12-amino acid fragment.an analysis of a series of five peptides composed of various portions of the pleurocidin (plc) sequence identified a l2-amino acid fragment from the c-terminus of plc, designated plc-2, as the smallest fragment that retained a antimicrobial activity comparable to that of the parent compound. mic tests in vitro with low-ionic-strength medium showed that plc-2 has potent activity against pseudomonas aeruginosa, escherichia coli and staphylococcus aureus but not against enterococcus faecalis. the a ...201323603258
characterization of an acid-tolerant β-1,4-glucosidase from fusarium oxysporum and its potential as an animal feed additive.an extracellular β-glucosidase (bgl) from fusarium oxysporum was purified to homogeneity by a single chromatography step on a gel filtration column. the optimum activity of bgl on cellobiose was observed at ph 5.0 and 60 °c. under the same conditions, the k(m) and v(max) values for p-nitrophenyl β-d-glucopyranoside and cellobiose were 2.53 mm, 268 u mg protein(-1) and 20.3 mm, 193 u mg protein(-1), respectively. the f. oxysporum bgl enzyme was highly stable at acidic ph (t 1/2 = 470 min at ph 3) ...201323604557
quantitative and microscopic assessment of compatible and incompatible interactions between chickpea cultivars and fusarium oxysporum f. sp. ciceris races.fusarium wilt caused by fusarium oxysporum f. sp. ciceris, a main threat to global chickpea production, is managed mainly by resistant cultivars whose efficiency is curtailed by fusarium oxysporum f. sp. ciceris races.201323613839
the endophytic strain fusarium oxysporum fo47: a good candidate for priming the defense responses in tomato roots.the protective fusarium oxysporum strain fo47 is effective in controlling fusarium wilt in tomato. previous studies have demonstrated the role of direct antagonism and involvement of induced resistance. the aim of the present study was to investigate whether priming of plant defense responses is a mechanism by which fo47 controls fusarium wilt. an in vitro design enabled inoculation of the tap root with fo47 and the pathogenic strain (fol8) at different locations and different times. the express ...201323617416
maldi-fticr ms imaging as a powerful tool to identify paenibacillus antibiotics involved in the inhibition of plant pathogens.nowadays, microorganisms are more and more often used as biocontrol agents for crop protection against diseases. among them, bacteria of bacillus and paenibacillus genders are already used as commercial biocontrol agents. their mode of action is supposed to be related to their production of antibiotics, such as cyclic lipopeptides, which exhibit great antimicrobial activities. we chose to work with a paenibacillus polymyxa strain (pp56) very resistant to various microorganisms. the bacteria were ...201323636858
molecular cloning and expression of five glutathione s-transferase (gst) genes from banana (musa acuminata l. aaa group, cv. cavendish).three tau class magsts responded to abiotic stress, magstf1 and magstl1 responded to signaling molecules, they may play an important role in the growth of banana plantlet. glutathione s-transferases (gst) are multifunctional detoxification enzymes that participate in a variety of cellular processes, including stress responses. in this study, we report the molecular characteristics of five gst genes (magstu1, magstu2, magstu3, magstf1 and magstl1) cloned from banana (musa acuminate l. aaa group, ...201323652818
synthesis and in vitro antifungal activities of new 2-aryl-6,7-methylenedioxy-3,4-dihydroisoquinolin-2-ium bromides.2-aryl-3,4-dihydroisoquinolin-2-iums might be considered as a class of simple analogues of natural quaternary benzo[c]phenanthridine alkaloids. in this paper, 26 new 2-aryl-6,7-methylenedioxy-3,4-dihydroisoquinolin-2-ium bromides with various substituents in n-aromatic ring were synthesized from commercially available 1,3-benzodioxole in good to excellent yields. all the compounds were elucidated by ms, high resolution (hr)-ms, ir, (1)h- and (13)c-nmr analysis, and evaluated for antifungal activ ...201323666271
heterologous expression of an aspartic protease gene from biocontrol fungus trichoderma asperellum in pichia pastoris.trichoderma asperellum parasitizes a large variety of phytopathogenic fungi. the mycoparasitic activity of t. asperellum depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall and proteases which are a group of enzymes capable of degrading proteins from host. in this study, a full-length cdna clone of aspartic protease gene, taasp, from t. asperellum was obtained and sequenced. the 1,185 bp long cdna sequence was predicted to encode a 395 amino acid ...201323677580
identification of volatile compounds produced by the bacterium burkholderia tropica that inhibit the growth of fungal pathogens.it has been documented that bacteria from the burkholderia genera produce different kinds of compounds that inhibit plant pathogens, however in burkholderia tropica, an endophytic diazotrophic and phosphate-solubilizing bacterium isolated from a wide diversity of plants, the capacity to produce antifungal compounds has not been evaluated. in order to expand our knowledge about burkholderia tropica as a potential biological control agent, we analyzed 15 different strains of this bacterium to eval ...201523680857
coniochaetones e-i, new 4h-chromen-4-one derivatives from the cordyceps-colonizing fungus fimetariella sp.five new 4h-chromen-4-one derivatives coniochaetones e-i (1-5), along with the known compounds coniochaetones b (6) and a (7) have been isolated from solid cultures of the cordyceps-colonizing fungus fimetariella sp. their structures were elucidated primarily by nmr spectroscopy and the absolute configurations of compounds 1-3 were assigned using the modified mosher's method. compound 4 showed weak cytotoxic activity against hela cells with ic50 values of 72.8 μm. the co-isolated known compound ...201323685047
antifungal attributes of siderophore produced by the pseudomonas aeruginosa jas-25.siderophores produced by pseudomonas sp. play a vital role in controlling several plant diseases. public concern about chemical pesticides has fostered an interest to seek alternative control strategies. production of siderophore by pseudomonas aeruginosa jas-25 in king b medium was estimated at 400 nm and was partially purified by gel filtration chromatography using sephadex g 25. antifungal activity and inhibition of spore germination was studied against the phytopathogens like fusarium oxyspo ...201423686966
effectiveness of dyes removal by mixed fungal cultures and toxicity of their metabolites.decolorization of brilliant green (0.06 g/l), evans blue (0.15 g/l), and their mixture (total concentration 0.08 g/l, proportion 1:1 w/w) by fungi was studied. fungal strains [pleurotus ostreatus (bwph), gloeophyllum odoratum (dca), and fusarium oxysporum (g1)] were used separately and as a mixture of them. zootoxicity (daphnia magna) and phytotoxicity (lemna minor) changes were estimated after the end of experiment. mixtures of fungal strains were less effective in decolorization process than t ...201323687394
root exudates from grafted-root watermelon showed a certain contribution in inhibiting fusarium oxysporum f. sp. niveum.grafting watermelon onto bottle gourd rootstock is commonly used method to generate resistance to fusarium oxysporum f. sp. niveum (fon), but knowledge of the effect of the root exudates of grafted watermelon on this soil-borne pathogen in rhizosphere remains limited. to investigate the root exudate profiles of the own-root bottle gourd, grafted-root watermelon and own-root watermelon, recirculating hydroponic culture system was developed to continuously trap these root exudates. both conidial g ...201323700421
systemic acquired resistance in cavendish banana induced by infection with an incompatible strain of fusarium oxysporum f. sp. cubense.fusarium wilt of banana is caused by the soil-borne fungus fusarium oxysporum f. sp. cubense (foc). the fact that there are no economically viable biological, chemical, or cultural measures of controlling the disease in an infected field leads to search for alternative strategies involving activation of the plant's innate defense system. the mechanisms underlying systemic acquired resistance (sar) are much less understood in monocots than in dicots. since systemic protection of plants by attenua ...201323702248
epi-trichosetin, a new undecaprenyl pyrophosphate synthase inhibitor, produced by fusarium oxysporum fki-4553.a new compound, designated epi-trichosetin (1), was isolated along with the known compound trichosetin (2) from the culture broth of fusarium oxysporum fki-4553 by solvent extraction, silica gel column chromatography and reversed-phase hplc. the structure of 1 was elucidated by comparing various spectral data with those of 2, revealing that 1 was a stereoisomer of 2. compounds 1 and 2 inhibited the undecaprenyl pyrophosphate synthase activity of staphylococcus aureus with ic50 values of 83 and 3 ...201323715038
hyperkeratotic warty skin lesion of foot caused by fusarium oxysporum.fusarium species are common soil-inhabiting organisms and plant pathogens. human infections are usually precipitated by local or systemic predisposing factors, and disseminated infection is associated with impaired immune responses. skin infections caused by fusarium spp. include keratitis, onychomycosis, mycetoma, painful discrete erythematous nodules. hyperkeratotic skin lesions caused by fusarium spp. are, however, rarely reported. we report a case of hyperkeratotic verrucous warty skin lesio ...201323716829
fungal endophytes from three cultivars of panax ginseng meyer cultivated in korea.in order to investigate the diversity of endophytes, fungal endophytes in panax ginseng meyer cultivated in korea were isolated and identified using internal transcribed spacer (its) sequences of ribosomal dna. three cultivars of 3-year-old ginseng roots (chunpoong, yunpoong, and gumpoong) were used to isolate fungal endophytes. surface sterilized ginseng roots were placed on potato dextrose agar plates supplemented with ampicilin and streptomycin to inhibit bacterial growth. overall, 38 fungal ...201223717111
arabidopsis thaliana resistance to fusarium oxysporum 2 implicates tyrosine-sulfated peptide signaling in susceptibility and resistance to root infection.in the plant arabidopsis thaliana, multiple quantitative trait loci (qtls), including rfo2, account for the strong resistance of accession columbia-0 (col-0) and relative susceptibility of taynuilt-0 (ty-0) to the vascular wilt fungus fusarium oxysporum forma specialis matthioli. we find that rfo2 corresponds to diversity in receptor-like protein (rlp) genes. in col-0, there is a tandem pair of rlp genes: rfo2/at1g17250 confers resistance while rlp2 does not. in ty-0, the highly diverged rfo2 lo ...201323717215
lipolytic system of the tomato pathogen fusarium oxysporum f. sp. lycopersici.the lipolytic profile of fusarium oxysporum f. sp lycopersici was studied by in silico search and biochemical enzyme activity analyses. twenty-five structural secreted lipases were predicted based on the conserved pentapeptide gly-x-ser-x-gly-, characteristic of fungal lipases, and secretion signal sequences. moreover, a predicted lipase regulatory gene was identified in addition to the previously characterized ctf1. the transcription profile of thirteen lipase genes during tomato plant coloniza ...201323718123
[fusariosis diagnosed in the laboratory of an uh in tunisia: epidemiological, clinical and mycological study].the genus fusarium, initially known for its important agro-economic impact, is more and more often implicated in human pathology. in fact, multiples allergic, toxic and infectious manifestations are more reported in immunocompetent and immunocompromised hosts. the objective of our study was to analyse the epidemiological, mycological and clinical features of fusariosis reported in our chu. eighty-seven cases of fusarium infections were collected: 34 cases of onychomycosis (39%), 26 cases of inte ...201323725904
antifungal activity and cytotoxicity of isolated compounds from leaves of breonadia salicina.breonadia salicina is used traditionally to treat wounds, ulcers, fevers, headaches, and fungal infections. the aim of this study was to investigate the antifungal activity of the plant extract and compounds isolated there from.201323727185
phenazine antibiotic production and antifungal activity are regulated by multiple quorum-sensing systems in pseudomonas chlororaphis subsp. aurantiaca stfrb508.a number of gram-negative bacteria have a quorum-sensing system and produce the n-acylhomoserine lactone (ahl) as a signal molecule. pseudomonas chlororaphis subsp. aurantiaca stfrb508 produces one of the phenazine derivatives, phenazine-1-carboxylic acid (pca). whole-genome sequencing of stfrb508 revealed the presence of two sets of ahl-synthase and ahl-receptor gene, phzir and aurir. the mutation of phzi drastically decreased pca production, but the mutation of auri did not affect pca producti ...201323727350
transformation of pwwo in rhizobium leguminosarum dpt to engineer toluene degrading ability for rhizoremediation.rhizoremediation of organic xenobiotics is based on interactions between plants and their associated micro-organisms. the present work was designed to engineer a bacterial system having toluene degradation ability along with plant growth promoting characteristics for effective rhizoremediation. pwwo harboring the genes responsible for toluene breakdown was isolated from pseudomonas putida mtcc 979 and successfully transformed in rhizobium dpt. this resulted in a bacterial strain (dpt(t)) which h ...201123729882
an iron 13s-lipoxygenase with an α-linolenic acid specific hydroperoxidase activity from fusarium oxysporum.jasmonates constitute a family of lipid-derived signaling molecules that are abundant in higher plants. the biosynthetic pathway leading to plant jasmonates is initiated by 13-lipoxygenase-catalyzed oxygenation of α-linolenic acid into its 13-hydroperoxide derivative. a number of plant pathogenic fungi (e.g. fusarium oxysporum) are also capable of producing jasmonates, however, by a yet unknown biosynthetic pathway. in a search for lipoxygenase in f. oxysporum, a reverse genetic approach was use ...201323741422
preparative thin-layer chromatographic separation followed by identification of antifungal compound in cassia laevigata by rp-hplc and gc-ms.several species of the genus cassia are known for their antioxidant, antimicrobial and antidiabetic activities, but some of the lesser-known cassia species, e.g. c. renigera, c. biflora and c. laevigata have not been studied for their biological activities.201423749313
fusariotoxins in asparagus - their biosynthesis and migration.asparagus is often infected by fungi of the fusarium genus, a causal agent of crown and root rot, which decreases the quantity and quality of spears. fusarium oxysporum and fusarium proliferatum are the most severe asparagus pathogens, well known as mycotoxin producers, mainly fumonisins and moniliformin. the present study was undertaken to estimate fumonisin b1, moniliformin and ergosterol concentrations in asparagus tissue. moreover, the possibility of toxin transport to the edible asparagus p ...201323756238
modified primers for the identification of nonpathogenic fusarium oxysporum isolates that have biological control potential against fusarium wilt of cucumber in taiwan.previous investigations demonstrated that fusarium oxysporum (fo), which is not pathogenic to cucumbers, could serve as a biological control agent for managing fusarium wilt of cucumber caused by fo f. sp. cucumerinum (foc) in taiwan. however, thus far it has not been possible to separate the populations of pathogenic fo from the nonpathogenic isolates that have biological control potential through their morphological characteristics. although these two populations can be distinguished from one ...201323762289
streptomyces rochei acta1551, an indigenous greek isolate studied as a potential biocontrol agent against fusarium oxysporum f.sp. lycopersici.many studies have shown that several greek ecosystems inhabit very interesting bacteria with biotechnological properties. therefore streptomyces isolates from diverse greek habitats were selected for their antifungal activity against the common phytopathogenic fungus fusarium oxysporum. the isolate encoded acta1551, member of streptomyces genus, could strongly suppress the fungal growth when examined in antagonistic bioassays in vitro. the isolate was found phylogenetically relative to streptomy ...201323762841
antifungal, insecticidal, and plant growth promoting potential of streptomyces hydrogenans dh16.in the present study, an actinobacterium strain, possessing antagonistic activity against different fungal phytopathogens viz. colletotrichum acutatum, cladosporium herbarum, alternaria brassicicola, exserohilum sp., alternaria mali, colletotrichum gleospoiroides, alternaria alternata, cercospora sp., fusarium oxysporum f.sp. dianthi and fusarium moniliformae, was isolated from soil and identified as streptomyces hydrogenans dh16. application of culture supernatant (5%)/cells (10(7)  cfu ml(-1) ...201423765423
antibacterial activity of extracellular compounds produced by a pseudomonas strain against methicillin-resistant staphylococcus aureus (mrsa) strains.the emergence of multidrug-resistant bacteria is a world health problem. staphylococcus aureus, including methicillin-resistant s. aureus (mrsa) strains, is one of the most important human pathogens associated with hospital and community-acquired infections. the aim of this work was to evaluate the antibacterial activity of a pseudomonas aeruginosa-derived compound against mrsa strains.201323773484
insights into fungal communities in composts revealed by 454-pyrosequencing: implications for human health and safety.fungal community composition in composts of lignocellulosic wastes was assessed via 454-pyrosequencing of its1 libraries derived from the three major composting phases. ascomycota represented most (93%) of the 27,987 fungal sequences. a total of 102 genera, 120 species, and 222 operational taxonomic units (otus; >97% similarity) were identified. thirty genera predominated (ca. 94% of the sequences), and at the species level, sequences matching chaetomium funicola and fusarium oxysporum were the ...201323785368
diversity in receptor-like kinase genes is a major determinant of quantitative resistance to fusarium oxysporum f.sp. matthioli.resistance to wilt fungus fusarium oxysporum f.sp. matthioli (fom) is a polygenic trait in arabidopsis thaliana. rfo3 is one of six quantitative trait loci accounting for the complete resistance of accession columbia-0 (col-0) and susceptibility of accession taynuilt-0 (ty-0). we find that col-0 and ty-0 alleles of rfo3 are representative of two common variants in wild arabidopsis accessions, that resistance and susceptibility to fom are ancestral features of the two variants and that resistance ...201323790083
identification and characterization of an anti-fungi fusarium oxysporum f. sp. cucumerium protease from the bacillus subtilis strain n7.a newly discovered alkaline antifungal protease named p6 from bacillus subtilis n7 was purified and partially characterized. b. subtilis n7 culture filtrates were purified by 30-60% (nh4)2so4 precipitation, anion-exchange chromatography and gel filtration chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) revealed a single band of 41.38 kda. peptide sequence of protease p6 was determined using a 4800 plus maldi tof/tof™ analyzer system. self-formed adaptor pcr ( ...201323812816
disseminated fusariosis secondary to neuroblastoma with fatal outcome.disseminated fusariosis is an uncommon clinical condition in immunocompromised patients. we report a fatal case of disseminated fusariosis secondary to neuroblastoma in a male patient, 15 years old, who underwent a bone marrow transplant. the patient was admitted to the pediatric intensive care unit (picu) of a public hospital in recife-pe, brazil, presenting bone marrow aplasia, severe leukopenia, and thrombocytopenia. after 15 days, the patient developed right knee effusion. synovial fluid and ...201323813153
analysis of hairpin rna transgene-induced gene silencing in fusarium oxysporum.hairpin rna (hprna) transgenes can be effective at inducing rna silencing and have been exploited as a powerful tool for gene function analysis in many organisms. however, in fungi, expression of hairpin rna transcripts can induce post-transcriptional gene silencing, but in some species can also lead to transcriptional gene silencing, suggesting a more complex interplay of the two pathways at least in some fungi. because many fungal species are important pathogens, rna silencing is a powerful te ...201323819794
optimization of media and temperature for enhanced antimicrobial production by bacteria associated with rhabditis sp.entomopathogenic nematodes, belonging to the family heterorhabditis and steinernematidae, are reported to be symbiotically associated with specific bacteria and the secondary metabolites produced by these bacteria possess antimicrobial activity. in this study, bacteria were isolated from nematodes belonging to the family rhabditidae, and the antimicrobial activity was tested against four bacteria viz. bacillus subtilis mtcc 2756, staphylococcus aureus mtcc 902, escherichia coli mtcc 2622, and ps ...201323825731
fusaric acid induction of programmed cell death modulated through nitric oxide signalling in tobacco suspension cells.fusaric acid (fa) is a nonhost-selective toxin mainly produced by fusarium oxysporum, the causal agent of plant wilt diseases. we demonstrate that fa can induce programmed cell death (pcd) in tobacco suspension cells and the fa-induced pcd is modulated by nitric oxide (no) signalling. cells undergoing cell death induced by fa treatment exhibited typical characteristics of pcd including cytoplasmic shrinkage, chromatin condensation, dna fragmentation, membrane plasmolysis, and formation of small ...201323838885
identification of immunity related genes to study the physalis peruviana--fusarium oxysporum pathosystem.the cape gooseberry (physalisperuviana l) is an andean exotic fruit with high nutritional value and appealing medicinal properties. however, its cultivation faces important phytosanitary problems mainly due to pathogens like fusarium oxysporum, cercosporaphysalidis and alternaria spp. here we used the cape gooseberry foliar transcriptome to search for proteins that encode conserved domains related to plant immunity including: nbs (nucleotide binding site), cc (coiled-coil), tir (toll/interleukin ...201323844210
targeting iron acquisition blocks infection with the fungal pathogens aspergillus fumigatus and fusarium oxysporum.filamentous fungi are an important cause of pulmonary and systemic morbidity and mortality, and also cause corneal blindness and visual impairment worldwide. utilizing in vitro neutrophil killing assays and a model of fungal infection of the cornea, we demonstrated that dectin-1 dependent il-6 production regulates expression of iron chelators, heme and siderophore binding proteins and hepcidin in infected mice. in addition, we show that human neutrophils synthesize lipocalin-1, which sequesters ...201323853581
proteomic identification of potential target proteins regulated by the scf(f) (bp1) -mediated proteolysis pathway in fusarium oxysporum.f-box proteins function in the recruitment of proteins for scf ubiquitination and proteasome degradation. here, we studied the role of fbp1, a nonessential f-box protein of the tomato pathogen fusarium oxysporum f. sp. lycopersici. the δfbp1 mutant showed a significant delay in the production of wilt symptoms on tomato plants and was impaired in invasive growth on cellophane membranes and on living plant tissue. to search for target proteins recruited by fbp1, a combination of sodium dodecylsulp ...201323855991
in vitro activity of miltefosine as a single agent and in combination with voriconazole or posaconazole against uncommon filamentous fungal pathogens.antifungal treatment of uncommon filamentous fungal infections is problematic. this study determined the in vitro susceptibility of miltefosine, as a single agent and in combination with posaconazole or voriconazole, against these pathogens.201323861311
comparative mapping of raphanus sativus genome using brassica markers and quantitative trait loci analysis for the fusarium wilt resistance trait.fusarium wilt (fw), caused by the soil-borne fungal pathogen fusarium oxysporum is a serious disease in cruciferous plants, including the radish (raphanus sativus). to identify quantitative trait loci (qtl) or gene(s) conferring resistance to fw, we constructed a genetic map of r. sativus using an f2 mapping population derived by crossing the inbred lines '835' (susceptible) and 'b2' (resistant). a total of 220 markers distributed in 9 linkage groups (lgs) were mapped in the raphanus genome, cov ...201323864230
structural and functional diversity of rhizobacteria associated with rauwolfia spp. across the western ghat regions of karnataka, india.the present study carried out with denaturing gradient gel electrophoresis of dna extracted from rhizosphere soils of rauwolfia spp. collected from western ghat (wg) regions of karnataka indicated that pseudomonas sp. was prevalently found followed by methylobacterium sp., bacillus sp. and uncultured bacteria. a total of 200 rhizobacteria were isolated from 58 rhizosphere soil samples comprising of 15 different bacterial genera. the shannon weaver diversity index (h') and simpson's diversity ind ...201423864441
plant defensin type 1 (pdf1): protein promiscuity and expression variation within the arabidopsis genus shed light on zinc tolerance acquisition in arabidopsis halleri.plant defensins are recognized for their antifungal properties. however, a few type 1 defensins (pdf1s) were identified for their cellular zinc (zn) tolerance properties after a study of the metal extremophile arabidopsis halleri. in order to investigate whether different paralogues would display specialized functions, the a. halleri pdf1 family was characterized at the functional and genomic levels. eleven pdf1s were isolated from a. halleri. their ability to provide zn tolerance in yeast cells ...201323865749
slow sand filters effectively reduce phytophthora after a pathogen switch from fusarium and a simulated pump failure.slow sand filtration has been shown to effectively reduce phytophthora zoospores in irrigation water. this experiment tested the reduction of phytophthora colony forming units (cfus) by slow sand filtration systems after switching the pathogen contaminating plant leachate from fusarium to phytophthora and the resilience of the system to a short period without water, as might be caused by a pump failure. the slow sand filtration system greatly reduced phytophthora cfus and transmission after swit ...201323866129
characterization of chlordecone-tolerant fungal populations isolated from long-term polluted tropical volcanic soil in the french west indies.the insecticide chlordecone is a contaminant found in most of the banana plantations in the french west indies. this study aims to search for fungal populations able to grow on it. an andosol heavily contaminated with chlordecone, perfused for 1 year in a soil-charcoal system, was used to conduct enrichment cultures. a total of 103 fungal strains able to grow on chlordecone-mineral salt medium were isolated, purified, and deposited in the miae collection (microorganismes d'intérêt agro-environne ...201423872892
the antagonism activity of bacteria isolated from potato cultivated soil.soil-borne fungal and bacterial root pathogens can cause serious losses to agricultural crops. resistant plant varieties are not available for several soil-borne pathogens and chemical control is often insufficiently effective in soil. the enhancement of disease suppressive properties of soils will limit disease development, thus, being of great importance for sustainable agriculture as well as organic farming systems. the aim of this research is to find and identify suppressive soils in the sét ...201223878982
characterization of antifungal activity of the gh-46 subclass iii chitosanase from bacillus circulans mh-k1.we characterized the antifungal activity of the bacillus circulans subclass iii mh-k1 chitosanase (mh-k1 chitosanase), which is one of the most intensively studied glycoside hydrolases (ghs) that belong to gh family 46. mh-k1 chitosanase inhibited the growth of zygomycetes fungi, rhizopus and mucor, even at 10 pmol (0.3 μg)/ml culture probably via its fungistatic effect. the amino acid substitution e37q abolished the antifungal activity of mh-k1 chitosanase, but retained binding to chitotriose. ...201323892828
biochemical markers assisted screening of fusarium wilt resistant musa paradisiaca (l.) cv. puttabale micropropagated clones.an efficient protocol was standardized for screening of panama wilt resistant musa paradisiaca cv. puttabale clones, an endemic cultivar of karnataka, india. the synergistic effect of 6-benzyleaminopurine (2 to 6 mg/l) and thidiazuron (0.1 to 0.5 mg/l) on ms medium provoked multiple shoot induction from the excised meristem. an average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/l 6-benzyleaminopurine and 0.3 mg/l thidiazuron concentrations. elongation of shoots observed on 5 mg/ ...201323898552
[effects of nitrogen application rate on faba bean fusarium wilt and rhizospheric microbial metabolic functional diversity].a field plot experiment was conducted to study the effects of different nitrogen (n) application rates on the microbial functional diversity in faba bean rhizosphere and the relationships between the microbial functional diversity and the occurrence of faba bean fusarium wilt. four nitrogen application rates were installed, i. e. , n0(0 kg hm-2 , n1 (56. 25 kg hm-2) , n2(112. 5 kg hm-2), and n3 (168.75 kg hm-2), and biolog microbial analysis system was applied to study the damage of faba bean fu ...201323898671
fusarium oxysporum infection assays in arabidopsis.increased susceptibility to fusarium oxysporum is one of the most conspicuous characteristics of the arabidopsis mutants lacking the heterotrimeric g protein β and γ1 subunits. the molecular mechanisms placing these g proteins in the plant innate immunity network are yet to be discovered. however, a method to test susceptibility to and disease progression of an important plant pathogen, such as f. oxysporum, is of central importance to many plant defense studies. the optimized protocol presented ...201323913036
contamination of bananas with beauvericin and fusaric acid produced by fusarium oxysporum f. sp. cubense.fusarium wilt, caused by the fungal pathogen fusarium oxysporum f. sp. cubense (foc), is one of the most destructive diseases of banana. toxins produced by foc have been proposed to play an important role during the pathogenic process. the objectives of this study were to investigate the contamination of banana with toxins produced by foc, and to elucidate their role in pathogenesis.201323922960
rhizobacterium-mediated growth promotion and expression of stress enzymes in glycine max l. merrill against fusarium wilt upon challenge inoculation.wilt disease of soybean caused by a very common soil-borne fungus, fusarium oxysporum is one of the most destructive diseases of the crop. the aim of the present study was to characterize plant growth-promotion activities and induced resistance of a rhizobacterial strain for the soybean plant against f. oxysporum. rhizobacterium strain sj-5 exhibited plant growth-promotion characteristics and antagonistic activity against the test pathogen on dual plate assay. it was identified as a carnobacteri ...201423933805
birth, death and horizontal transfer of the fumonisin biosynthetic gene cluster during the evolutionary diversification of fusarium.fumonisins are a family of carcinogenic secondary metabolites produced by members of the fusarium fujikuroi species complex (ffsc) and rare strains of fusarium oxysporum. in fusarium, fumonisin biosynthetic genes (fum) are clustered, and the cluster is uniform in gene organization. here, sequence analyses indicated that the cluster exists in five different genomic contexts, defining five cluster types. in fum gene genealogies, evolutionary relationships between fusaria with different cluster typ ...201323937442
effects of electrokinetic operation mode on removal of polycyclic aromatic hydrocarbons (pahs), and the indigenous fungal community in pah-contaminated soil.electrokinetic remediation is an emerging physical remediation technology for the removal of heavy metals and organic chemicals from contaminated soil. we set up a soil chamber (24 × 12 × 8 cm) with two stainless steel electrodes (12 × 0.5 cm), and a constant voltage gradient of 1.0 v cm(-1) or 2.0 v cm(-1) was applied to study the effects of unidirectional and altered directional electric field operation modes on the moisture content and ph, the removal rate of pahs, and the abundance and diver ...201323947706
use of a passive bioreactor to reduce water-borne plant pathogens, nitrate, and sulfate in greenhouse effluent.the goal of this study was to evaluate the use of passive bioreactors to reduce water-borne plant pathogens (pythium ultimum and fusarium oxysporum) and nutrient load (no(-) 3 and so(2-) 4) in greenhouse effluent. sterilized and unsterilized passive bioreactors filled with a reactive mixture of organic carbon material were used in three replicates. after a startup period of 2 (sterilized) or 5 (unsterilized) weeks, the bioreactor units received for 14 weeks a reconstituted commercial greenhouse ...201323947714
characterization of a β-1,3-glucan recognition protein from the beet armyworm, spodoptera exigua (insecta: lepidoptera: noctuidae).the β-1,3-glucan recognition protein gene from spodoptera exigua (seβgrp) was cloned and characterized. the cdna of this gene is 1 644 nucleotides in length and the predicted polypeptide is 491 amino acids (aa) in length, with a calculated molecular mass of 54.8 kda. the first 22 aa encode a predicted secretion signal peptide. a blast search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of seβgrp revealed that this protein is most similar to the β-1,3-glucan recognit ...201323956146
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...201123961146
construction of a new gfp vector and its use for fusaruim oxysporum transformation.in this study, the gfp fragment as a reporter gene had integrated into the form plasmid vector pbc-hygro which contains an expressive promoter of the fungus to facilitate the transformation of fusarium oxysporum. the resultant plasmid pbc-hygro-gfp was identified by digestion with enzymes. binary plasmids pbc-hygro-gfp were transformed into f. oxysporum by using the peg-cacl2 mediated transformation technique. results show that the recombinant plasmid pbc-hygro-gfp was constructed correctly. the ...201223961215
cross-talk interactions of exogenous nitric oxide and sucrose modulates phenylpropanoid metabolism in yellow lupine embryo axes infected with fusarium oxysporum.the aim of the study was to examine cross-talk of exogenous nitric oxide (no) and sucrose in the mechanisms of synthesis and accumulation of isoflavonoids in embryo axes of lupinus luteus l. cv. juno. it was verified whether the interaction of these molecules can modulate the defense response of axes to infection and development of the pathogenic fungus fusarium oxysporum f. sp. lupini. sucrose alone strongly stimulated a high level of genistein glucoside in axes pretreated with exogenous nitric ...201323987816
molecular detection and genotyping of fusarium oxysporum f. sp. psidii isolates from different agro-ecological regions of india.twenty one isolates of fusarium oxysporum f. sp. psidii (fop), causing a vascular wilt in guava (psidium guajava l.), were collected from different agro-ecological regions of india. the pathogenicity test was performed in guava seedlings, where the fop isolates were found to be highly pathogenic. all 21 isolates were confirmed as f. oxysporum f. sp. psidii by a newly developed, species-specific primer against the conserved regions of 28s rdna and the intergenic spacer region. rapd and pcr-rflp w ...201323990290
ecological and physiological studies on soil fungi at western region, libya.sixty three species and 5 varieties belonging to 30 fungal genera were collected from 75 soil samples. cultivated (29 genera and 58 species + 5 var.), desert (22 and 35 + 2 var.) and saline soil (21 and 41 + 1 var.) fungi were recovered on glucose-, cellulose- and 50% sucrose-czapek's agar at 28℃. the most common genera were alternaria, aspergillus, emericella, fusarium, mycosphaerella, nectria and penicillium. the most prevalent species from the three types of soils on the three types of media ...200823997599
effect of some food preservatives on the lipolytic activity of beef luncheon fungi.beef luncheon meat is one of the most popular meals in several countries in the world including egypt. thirty one fungal species and 3 species varieties were recovered from 30 samples of beef luncheon meat collected from different supermarkets in qena. alternaria, aspergillus, emericella, mucor, mycosphaerella, penicillium and rhizopus were the most common genera on the two types of media. from the above genera, the most prevalent species were alternaria alternate, aspergillus flavus, a. fumigat ...200823997619
inhibitory effect of algal extracts on mycelial growth of the tomato-wilt pathogen, fusarium oxysporum f. sp. lycopersici.the present study was undertaken to explore the inhibitory effect of cyanobacterial extracts of nostoc commune fa-103 against the tomato-wilt pathogen, fusarium oxysporum f. sp. lycopersici. in an optimal medium, cell growth, antifungal activity, and antifungal compound production could be increased 2.7-fold, 4.1-fold, and 13.4-fold, respectively. a crude algal extract had a similar effect as mancozeb at the recommended dose, both in laboratory and pot tests. in vitro and in vivo fungal growth, ...200823997634
[bletilla striata pseudobulb rot pathogen identification and its biological characteristic].to identify the pathogen of bletilla striata pseudobulb rot and provide foundation for formulation effective control measures.201324010310
characterization of fusarium oxysporum isolated from paprika in korea.in the present study we first report in korea the identification and characterization of fusarium oxysporum isolated from rotten stems and roots of paprika (capsicum annuum var. grossum) at masan, kyungsangnamdo in 2006. the fungal species produced white aerial mycelia accompanying with dark violet pigment on pda. the optimal temperature and ph for the growth of the species was 25℃ and ph 7, respectively. microscopic observation of one of isolates of the species shows that its conidiophores are ...200724015078
inhibition of cereal rust fungi by both class i and ii defensins derived from the flowers of nicotiana alata.defensins are a large family of small, cysteine-rich, basic proteins, produced by most plants and plant tissues. they have a primary function in defence against fungal disease, although other functions have been described. this study reports the isolation and characterization of a class i secreted defensin (nad2) from the flowers of nicotiana alata, and compares its antifungal activity with the class ii defensin (nad1) from n. alata flowers, which is stored in the vacuole. nad2, like all other c ...201424015961
tandem combination of trigonella foenum-graecum defensin (tfgd2) and raphanus sativus antifungal protein (rsafp2) generates a more potent antifungal protein.plant defensins are small (45 to 54 amino acids) positively charged antimicrobial peptides produced by the plant species, which can inhibit the growth of a broad range of fungi at micro-molar concentrations. these basic peptides share a common characteristic three-dimensional folding pattern with one α-helix and three β-sheets that are stabilized by eight disulfide-linked cysteine residues. instead of using two single-gene constructs, it is beneficial when two effective genes are made into a sin ...201324022215
fusarium pathogenomics.fusarium is a genus of filamentous fungi that contains many agronomically important plant pathogens, mycotoxin producers, and opportunistic human pathogens. comparative analyses have revealed that the fusarium genome is compartmentalized into regions responsible for primary metabolism and reproduction (core genome), and pathogen virulence, host specialization, and possibly other functions (adaptive genome). genes involved in virulence and host specialization are located on pathogenicity chromoso ...201324024636
isolation, evaluation and characterization of bacillus subtilis from cotton rhizospheric soil with biocontrol activity against fusarium oxysporum.present investigation is based on the isolation of bacillus subtilis from cotton rhizosphere and their evaluation as biocontrol agent against fusarium oxysporum. the production of extracellular hydrolytic enzyme was studied for determining the antagonism. 43% of 21 isolates were identified under the b. subtilis group on the basis of biochemical characterization. 38% isolates showed competitive activity against fusarium oxysporum and exhibit more than 50% mycelial inhibition in dual culture bioas ...201024026922
synthesis and bioactivity evaluation of novel arylimines containing a 3-aminoethyl-2-[(p-trifluoromethoxy)anilino]-4(3h)-quinazolinone moiety.twenty-seven novel (e)-3-[2-arylideneaminoethyl]-2-[4-(trifluoromethoxy)anilino]-4(3h)-quinazolinone derivatives were synthesized by reacting various aromatic aldehydes with intermediate 6. the target compounds were characterized by (1)h nmr, (3)c nmr, ir, and elemental analysis. bioassay results revealed that some of the compounds have strong antifungal activities against six fungi ( gibberella zeae , fusarium oxysporum , clematis mandshurica , paralepetopsis sasakii , phytophthora infestans , ...201324028303
inoculum padronization for the production of cutinase by fusarium oxysporum.cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. the widespread use of this enzyme depends on the development of an efficient and low-cost production system. one of the most important steps in a fermentation process is the standardization of the inoculum characteristics. in this study, the production of cutinase by fusarium oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum pda ph. t ...200824031183
identification of an antifungal metabolite produced by a potential biocontrol actinomyces strain a01.actinomyces strain a01 was isolated from soil of a vegetable field in the suburb of beijing, china. according to the morphological, cultural, physiological and biochemical characteristics, and 16s rdna sequence analysis, strain a01 was identified as streptomyces lydicus. in the antimicrobial spectrum test strain a01 presented a stable and strong inhibitory activity against several plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, monilinia laxa, etc. however, no antibacterial ...200824031293
biological activities of a mixture of biosurfactant from bacillus subtilis and alkaline lipase from fusarium oxysporum.in this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from bacillus subtilis and an alkaline lipase from fusarium oxysporum (al/bs mix) on several types of microorganisms, as well as their abilities to remove listeria innocua atcc 33093 biofilm from stainless steel coupons. the al/bs mix had a surface tension of around 30 mn.m(-1), indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component. t ...201124031642
inoculation of tomato seedlings with trichoderma harzianum and arbuscular mycorrhizal fungi and their effect on growth and control of wilt in tomato seedlings.a green house study was conducted to investigate the ability of an isolate of trichoderma harzianum (p52) and arbuscular mycorrhizal fungi (amf) in enhancing growth and control of a wilt pathogen caused by fusarium oxysporum f. sp. lycopersici in tomato seedlings. the plants were grown in plastic pots filled with sterilized soils. there were four treatments applied as follows; p52, amf, amf + p52 and a control. a completely randomized design was used and growth measurements and disease assessmen ...201124031662
evaluation of antifungal activity in essential oil of the syzygium aromaticum (l.) by extraction, purification and analysis of its main component eugenol.antifungal properties of some essential oils have been well documented. clove oil is reported to have strong antifungal activity against many fungal species. in this study we have evaluated antifungal potential of essential oil of syzygium aromaticum (l.) against some common fungal pathogens of plants and animals namely, fusarium moniliforme ncim 1100, fusarium oxysporum mtcc 284, aspergillus sp., mucor sp., trichophyton rubrum and microsporum gypseum. all fungal species were found to be inhibit ...201124031751
surveillance of bacterial and fungal infections in the postoperative period following liver transplantation: a series from 2005-2011.orthotopic liver transplantation (olt) is a life-saving procedure for the treatment of many end-stage diseases, but infectious and acute rejection episodes remain major causes of morbidity and mortality. bacterial and fungal infections can be due to intra-abdominal, biliary, respiratory, urinary, wound, central venous catheters (cvc) or unknown sources. using the computerized database of our microbiology laboratory, we analyzed all the bacterial and fungal infections in the first three months fo ...201324034031
fusarium keratitis in brazil: genotyping, in vitro susceptibilities, and clinical outcomes.the purpose of this paper is to describe clinical characteristics and determine correlations between clinical outcomes and antifungal susceptibility among molecularly characterized ocular fusarium isolates in brazil.201324039389
comparison of rapd, aflp, and ef-1α sequences for the phylogenetic analysis of fusarium oxysporum and its formae speciales in korea.although fursarium oxysporum causes diseases in economically important plant hosts, identification of f. oxysporum formae speciales has been difficult due to confusing phenotypic classification systems. to resolve these complexity, we evaluated genetic relationship of nine formae speciales of f. oxysporum with random amplified polymorphic dna (rapd), amplified fragment length polymorphism (aflp), and translation elongation factor-1 alpha (ef-1α) gene. in addition, the correlation between mycotox ...200624039470
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