Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| steroids and an oxylipin from an algicolous isolate of aspergillus flavus. | a new oxylipin, (8e,12z)-10,11-dihydroxyoctadeca-8,12-dienoic acid (1), a new steroid, 3ß,4a-dihydroxy-26-methoxyergosta-7,24(28)-dien-6-one (2), and four known steroids, episterol (3), (22e,24r)-ergosta-7,22-dien-3ß,5a,6a-triol (4), (22e,24r)-ergosta-5,22-dien-3ß-ol (5), and (22e,24r)-ergosta-4,6,8(14),22-tetraen-3-one (6), were isolated from the cultures of aspergillus flavus, an endophytic fungus isolated from the marine red alga corallina officinalis. their structures and relative stereochem ... | 2011 | 21452344 |
| glutathione reductase/glutathione is responsible for cytotoxic elemental sulfur tolerance via polysulfide shuttle in fungi. | fungi that can reduce elemental sulfur to sulfide are widely distributed, but the mechanism and physiological significance of the reaction have been poorly characterized. here, we purified elemental sulfur-reductase (sr) and cloned its gene from the elemental sulfur-reducing fungus fusarium oxysporum. we found that nadph-glutathione reductase (gr) reduces elemental sulfur via glutathione as an intermediate. a loss-of-function mutant of the sr/gr gene generated less sulfide from elemental sulfur ... | 2011 | 21474441 |
| synthesis of a natural gamma-butyrolactone from nerylacetone by acremonium roseum and fusarium oxysporum cultures. | natural gamma-butyrolactone - (4r, 5r)-5-(4'-methyl-3'pentenyl)-4-hydroxy-5-methyl-dihydrofuran-2-one (2) was isolated as the product of microbial transformation of nerylacetone (1) by fungal strains. this product was obtained as the enantiomer (+) in high yields 24% and 61% with ee=94% and 82% by the biotransformation in the cultures of acremonium roseum am336 and fusarium oxysporum am13 respectively. | 2011 | 21485276 |
| synthesis and anti-microbial evaluation of some novel 1,2,4-triazole derivatives. | triazoles with different substituent groups are found to possess diverse applications in the field of medicine and industry. a series of 4-(substituted ethanoyl)amino-3-mercapto-5-(4-nitro)phenyl-1,2,4-triazoles (nu-1 to nu-15) were synthesized as novel antimicrobial agents starting from 4-nitrobenzoic acid. the chemical structures of these newly synthesized compounds were elucidated by ir, 1h nmr, 13c nmr, fab+ -ms spectral data and elemental analysis. their antimicrobial activities against sta ... | 2011 | 21485294 |
| predicting methyl iodide emission, soil concentration, and pest control in a two-dimensional chamber system. | due to ever-increasing state and federal regulations, the future use of fumigants is predicted on reducing negative environmental impacts while offering sufficient pestcontrol efficacy. to foster the development of a best management practice, an integrated tool is needed to simultaneously predict fumigant movement and pest control without having to conduct elaborate and costly experiments. the objective of this study was (i) to present a two-dimensional (2-d) mathematical model to describe both ... | 2011 | 21488499 |
| synthesis and antimicrobial activity of some new 4-hetarylpyrazole and furo[2,3-c]pyrazole derivatives. | in continuation of our efforts to find a new class of antimicrobial agents, a series of 4-hetarylpyrazoles and furo[2,3-c]pyrazoles were prepared via the reaction of 2-chloro-1-(5-hydroxy-3-methyl-1-phenyl-1h-pyrazol-4-yl)ethanone (1) with an appropriate nucleophilic reagents. these compounds were screened for their antibacterial activity against gram-positive bacteria (bacillus subtilis and bacillus thuringiensis), gram-negative bacteria (escherichia coli and pseudomonas aeruginosa) and antifun ... | 2011 | 21489661 |
| functional alteration of a dimeric insecticidal lectin to a monomeric antifungal protein correlated to its oligomeric status. | allium sativum leaf agglutinin (asal) is a 25-kda homodimeric, insecticidal, mannose binding lectin whose subunits are assembled by the c-terminal exchange process. an attempt was made to convert dimeric asal into a monomeric form to correlate the relevance of quaternary association of subunits and their functional specificity. using swiss-model program a stable monomer was designed by altering five amino acid residues near the c-terminus of asal. | 2011 | 21490929 |
| nuclear magnetic resonance (nmr) studies on the biosynthesis of fusaric acid from fusarium oxysporum f. sp. vasinfectum. | fusarium oxysporum is a fungal pathogen that attacks many important plants. uniquely pathogenic strains of f. oxysporum f. sp. vasinfectum were inadvertently imported into the united states on live cottonseed for dairy cattle feed. these strains produce exceptionally high concentrations of the phytotoxin fusaric acid. thus, fusaric acid may be a critical component in the pathogenicity of these biotypes. this study investigated the biosynthesis of fusaric acid using (13)c-labeled substrates inclu ... | 2011 | 21495723 |
| chemical constituents from endophytic fungus fusarium oxysporum. | a new oxysporidinone analogue (1) and a new 3-hydroxyl-2-piperidinone derivative (2), along with the known compounds (-)-4,6'-anhydrooxysporidinone (3), (+)-fusarinolic acid (4), gibepyrone d (5), beauvercin (6),cerevisterol (7), fusaruside (8), and (2s,2'r,3r,3'e,4e,8e)-1-o-d-glucopyranosyl-2-n-(2'-hydroxy-3'-octadecenoyl)-3-hydroxy-9-methyl-4,8-sphingadienine (9) were isolated from fusarium oxysporum. compounds 1-9 were evaluated for cytotoxicity using the mtt method against cancer cell lines, ... | 2011 | 21497643 |
| selection and differentiation of bacillus spp. antagonistic to fusarium oxysporum f.sp. lycopersici and alternaria solani infecting tomato. | antagonistic bacillus spp. displaying in vitro production of siderophore, chitinase, and β-1,3-glucanase were identified from dual culture assays. in independent greenhouse studies, seed bacterization and soil application of bacillus atrophaeus s2bc-2 challenge inoculated with fusarium oxysporum f.sp. lycopersici (fol) and alternaria solani (as) recorded low percent disease index of 25.3 and 28.7, respectively, over nonbacterised pathogen control (44.3 and 56.4). the low disease incidence corrob ... | 2011 | 21503737 |
| immunohistochemical analysis of cell wall hydroxyproline-rich glycoproteins in the roots of resistant and susceptible wax gourd cultivars in response to fusarium oxysporum f. sp. benincasae infection and fusaric acid treatment. | hydroxyproline-rich glycoproteins (hrgps) play a defensive role in host-pathogen interactions. however, specific roles of individual hrgps in plant defense against pathogen are poorly understood. changes in extracellular distribution and abundance of individual cell wall hrgps were investigated on root sections of two wax gourd (benincasa hispida cogn.) cultivars (fusarium wilt resistant and susceptible, respectively), which were analyzed by immunolabelling with 20 monoclonal antibodies recogniz ... | 2011 | 21505833 |
| growth responses of in vitro fusarium oxysporum f. sp. niveum to external supply of tannic acid. | allelochemicals released from root exudates or decaying residues of plants play diversified roles in ecological interactions of plant-pathogen. the objective of this work was to evaluate the allelopathic effect of an externally supplied tannic acid on soil-borne in vitro fusarium oxysporum f. sp. niveum. results showed that the tannic acid decreased the growth of the fungus up to 9.5% at 800 mg l(-1). conidial germination was reduced by 52.3% in comparison with the control. however, sporulation ... | 2010 | 21506492 |
| asymmetric total synthesis of 6-tuliposide b and its biological activities against tulip pathogenic fungi. | the structure-activity relationship was investigated to evaluate the antifungal activities of tuliposides and tulipalins against tulip pathogenic fungi. 6-tuliposide b was effectively synthesized via the asymmetric baylis-hillman reaction. tuliposides and tulipalins showed antifungal activities against most of the strains tested at high concentrations (2.5 mm), while botrytis tulipae was resistant to tuliposides. tulipalin formation was involved in the antifungal activity, tulipalin a showed hig ... | 2011 | 21512240 |
| fungistatic activity of bicyclo[4.3.0]-γ-lactones. | five optically active and sixteen racemic lactones (nine of them new) of bicyclo[4.3.0]nonane structure were synthesized. ic(50) values for the following phytopathogens were determined: aspergillus ochraceus am 456, fusarium culmorum am 282, fusarium oxysporum am 13, fusarium tricinctum am 16. effect of compound structures, especially stereogenic centers, on fungistatic activity has been discussed. the highest fungistatic activity was observed for trans-7,8-dibromo-cis-3-oxabicyclo[4.3.0]nonan-2 ... | 2011 | 21520970 |
| synthesis, structure and biological activity of novel 1,2,4-triazole mannich bases containing a substituted benzylpiperazine moiety. | a series of novel mannich bases with trifluoromethyl-1,2,4-triazole and substituted benzylpiperazine moieties were synthesized. their structures were confirmed by ir, (1) h nmr and elemental analysis. the single crystal structure of compound 4r was also determined. the preliminary bioassays showed that most of the lead compounds had low herbicidal activity against brassica campestris, echinochloa crusgalli, and kari enzyme. however, most of them exhibited significant fungicidal activity at the d ... | 2011 | 21521489 |
| rhizosphere soil microorganism populations and community structures of different watermelon cultivars with differing resistance to fusarium oxysporum f. sp. niveum. | fusarium wilt is an increasingly serious disease of watermelon that reduces crop productivity. changes in microorganism populations and bacterial and fungal community structures in rhizosphere soil of watermelon cultivars resistant or susceptible to fusarium oxysporum f. sp. niveum were investigated using a plate culture method and pcr-dgge analysis. plate culture showed that populations of culturable bacteria and actinomycetes were more abundant in the rhizosphere of the resistant watermelon cu ... | 2011 | 21529122 |
| mapping fusarium wilt race 1 resistance genes in cotton by inheritance, qtl and sequencing composition. | knowledge of the inheritance of disease resistance and genomic regions housing resistance (r) genes is essential to prevent expanding pathogen threats such as fusarium wilt [fusarium oxysporum f.sp. vasinfectum (fov) atk. sny & hans] in cotton (gossypium spp.). we conducted a comprehensive study combining conventional inheritance, genetic and quantitative trait loci (qtl) mapping, qtl marker-sequence composition, and genome sequencing to examine the distribution, structure and organization of di ... | 2011 | 21533837 |
| efficacy of microorganisms selected from compost to control soil-borne pathogens. | suppression of soil-borne plant pathogens with compost has been widely studied. compost has been found to be suppressive against several soil-borne pathogens in various cropping systems. however, an increase of some diseases due to compost usage has also been observed, since compost is a product that varies considerably in chemical, physical and biotic composition, and, consequently, also in ability to suppress soil borne diseases. new opportunities in disease management can be obtained by the s ... | 2010 | 21534476 |
| antifungal activity of rhizospheric bacteria. | fluorescent pseudomonad spp. were isolated from the rhizosphere of potato plants (algeria) by serial dilutions of rhizosphere soils on kings b medium and were tested for their antifungal activity. the antifungal activity of the pseudomonas isolated from potatoes rhizosphere was tested against pythium ultimum, rhizoctonia solani and fusarium oxysporum in dual culture with bacteria on pda. the petri dish was divided into tow, on one the bacteria was spread and on the opposite side fungal plugs wer ... | 2010 | 21534477 |
| inactivation of snt2, a bah/phd-containing transcription factor, impairs pathogenicity and increases autophagosome abundance in fusarium oxysporum. | the soil-borne, asexual fungus fusarium oxysporum f.sp. melonis (fom) is a causal agent of muskmelon wilt disease. the current study focused on the most virulent race of fom-race 1,2. the tagged mutant d122, generated by agrobacterium tumefaciens-mediated transformation, caused the delayed appearance of initial wilt disease symptoms, as well as a 75% reduction in pathogenicity. d122 was impaired in the gene product homologous to the snt2-like transcription factor of schizosaccharomyces pombe. in ... | 2011 | 21535351 |
| innate immune responses of a scleractinian coral to vibriosis. | scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are amongst the most diverse of all ecosystems. over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographical widespread and a major threat to this ecosystem. although coral immunity has been the subject of increasing study, the available ... | 2011 | 21536670 |
| indicator organisms for assessing sanitization during composting of plant wastes. | the potential for using plant pathogens and seeds as indicator organisms for assessing sanitization of plant wastes during composting was tested in bench-scale flask and large-scale systems. plasmodiophora brassicae was unsuitable due to high temperature tolerance in dry to moist composts, and detection of viable inoculum post-composting using bioassay plants not corresponding with that using taqman® pcr, possibly due to preservation of nucleic acids at elevated temperatures. several other plant ... | 2011 | 21546235 |
| identification and evaluation of two diagnostic markers linked to fusarium wilt resistance (race 4) in banana (musa spp.). | fusarium wilt caused by the fungus fusarium oxysporum f. sp. cubense race 4 (foc4) results in vascular tissue damage and ultimately death of banana (musa spp.) plants. somaclonal variants of in vitro micropropagated banana can hamper success in propagation of genotypes resistant to foc4. early identification of foc4 resistance in micropropagated banana plantlets is difficult, however. in this study, we identified sequence-characterized amplified region (scar) markers of banana associated with re ... | 2011 | 21547366 |
| fungal communities in herbaceous medicinal plants from the malnad region, southern india. | fungal communities were isolated from surface sterilized leaf segments of nine ethnopharmaceutically important medicinal herbs collected from the bhadra river project area, the malnad region, southern india. a total of 2159 isolates belonging to 55 different fungal species were isolated from 3600 leaf segments collected during the wet and dry seasons. chaetomium globosum (7.3%), aureobasidium pullulans (6.1%), cladosporium cladosporioides (3.9%), curvularia lunata (1.9%), nigrospora oryzae (1.7% ... | 2008 | 21558683 |
| effect of three organophosphorous nematicides on non-target nematodes and soil microbial community. | the toxicity of three organophosphorous nematicides, imicyafos, fosthiazate and cadusafos, to non-target organisms in soil was evaluated. imicyafos and fosthiazate had no significant inhibitory effect on the growth of fungal (fusarium oxysporum f. sp. lactucae, rhizoctonia solani and trichoderma viride) and bacterial (ralstonia solanacearum and pseudomonas fluorescens) strains in media at 12.5 to 200 mg l(-1). cadusafos, however, significantly inhibited the growth of all these strains except r. ... | 2008 | 21558727 |
| variation and phylogeny of fusarium oxysporum isolates based on nucleotide sequences of polygalacturonase genes. | the nucleotide sequences of two endopolygalacturonase genes (pg1 and pg5) and two exopolygalacturonase genes (pgx1 and pgx4), which encode members of a major family of secreted cell-wall-degrading enzymes (cwdes), were compared to detect the extent of genetic variation among isolates of fusarium oxysporum. the nucleotide variation rate in exons was 0.23-0.93%, higher than that in introns (0.01-0.64%) and untranslated regions (utrs) (0.07-0.25%), resulting in 0.05-0.31% variation in amino acids. ... | 2009 | 21566363 |
| antifungal properties of wheat histones (h1-h4) and purified wheat histone h1. | wheat ( triticum spp.) histones h1, h2, h3, and h4 were extracted, and h1 was further purified. the effect of these histones on specific fungi that may or may not be pathogenic to wheat was determined. these fungi included aspergillus flavus , aspergillus fumigatus , aspergillus niger , fusarium oxysporum , fusarium verticillioides , fusarium solani , fusarium graminearum , penicillium digitatum , penicillium italicum , and greeneria uvicola . non-germinated and germinating conidia of these fung ... | 2011 | 21595494 |
| isolation, selection and characterization of root-associated growth promoting bacteria in brazil pine (araucaria angustifolia). | araucaria angustifolia, a unique species of this genus that occurs naturally in brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. root-associated bacteria from a. angustifolia were isolated, selected and characterized for their biotechnological potential of growth promotion and biocontrol of plant pathogenic fungi. ninety-seven strains were isolated and subje ... | 2011 | 21596540 |
| natural resistance of rose petals to microbial attack. | petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ... | 2011 | 21614712 |
| application of inorganic carrier-based formulations of fluorescent pseudomonads and piriformospora indica on tomato plants and evaluation of their efficacy. | aims: fluorescent pseudomonads are widely used as bioinoculants for improving plant growth and controlling phytopathogenic fungi. piriformospora indica, a symbiotic root endophyte, also has beneficial effects on a number of plants. the present study focuses on improvement of growth yields of tomato plants and control of fusarium wilt using inorganic carrier- based formulations of two fluorescent pseudomonad strains (r62 and r81) and piriformospora indica. methods and results: the inorganic car ... | 2011 | 21624018 |
| infection of the sunagoke moss panels with fungal pathogens hampers sustainable greening in urban environments. | drought and heat tolerance of the sunagoke moss (racomitrium japonicum) and the low thermal conductivity of the dry moss tissue offer novel greening and insulation possibilities of roofs and walls to mitigate the heat island phenomenon in urban environments. however, damage may appear in the moss panels under humid conditions in japan. in this study we characterized fungi associated with the damaged areas of the sunagoke moss panels. fungi were identified by morphology and internal transcribed s ... | 2011 | 21624641 |
| isolation and characterization of two strains of fusarium oxysporum causing potato dry rot in solanum tuberosum in colombia. | background: fusarium oxysporum has worldwide distribution and causes severe vascular wilt or root rot in many plants. strains are classified into formae speciales based on their high degree of host specificity, of which multilocus sequence typing provides a fairly good estimate. aims: the main aim of this study was to identify the causal agent of an infected potato tuber in colombia. methodology and results: two f. oxysporum isolates were recovered from a potato tuber showing symptoms of dry rot ... | 2011 | 21635960 |
| a proteomic study of in-root interactions between chickpea pathogens: the root-knot nematode meloidogyne artiellia and the soil-borne fungus fusarium oxysporum f. sp. ciceris race 5. | fusarium wilt caused by the fungus fusarium oxysporum f. sp. ciceris (foc) is the main soil-borne disease limiting chickpea production. management of this disease is achieved mainly by the use of resistant cultivars. however, co-infection of a foc-resistant plant by the fungus and the root-knot nematode meloidogyne artiellia (ma) causes breakdown of the resistance and thus limits its efficacy in the control of fusarium wilt. in this work we aimed to reveal key aspects of chickpea:foc:ma interact ... | 2011 | 21640211 |
| changes and induction of aminopeptidase activities in response to pathogen infection during germination of pigeonpea (cajanas cajan) seeds. | aminopeptidases play important role in the mobilization of storage proteins at the cotyledon during seed germination. it is often referred as inducible component of defense against herbivore attack. however the role of aminopeptidase in response to pathogen attack in germinating seeds is remained to be unknown. an attempt was made to analyze change in the aminopeptidase (ec 3.4.11.1) activity during germination of pigeonpea (cajanus cajan l.) seeds by infecting the seeds with fungi. two aminopep ... | 2011 | 21640431 |
| molecular characterization of the modular chitin binding protein cbp50 from bacillus thuringiensis serovar konkukian. | bacillus thuringiensis is an insecticidal bacterium whose chitinolytic system may be exploited to improve the insecticidal system of bt-crops. a nucleotide fragment of 1368 bp from b. thuringiensis serovar konkukian s4, containing the complete coding sequence of the chitin binding protein cbp50, was cloned and sequenced. analyses have shown the protein to contain a modular structure consisting of an n-terminal cbm33 domain, two copies of a fibronectin-like domain and a c-terminal chitin binding ... | 2011 | 21647612 |
| development of a strain-specific real-time pcr assay for the detection and quantification of the biological control agent fo47 in root tissues. | being able to identify specifically a biological control agent at the strain level is not the only requirement set by regulations (ec)1107/2009, it is also necessary to study the interactions of the agent with the plant and the pathogen in the rhizosphere. fo47 is a soil-borne strain of fusarium oxysporum which has the capacity to protect several plant species against the pathogenic formae speciales of f. oxysporum inducing wilts. a strain-specific sequence-characterized amplified region marker ... | 2011 | 21658107 |
| a comparison of wild-type, old and modern tomato cultivars in the interaction with the arbuscular mycorrhizal fungus glomus mosseae and the tomato pathogen fusarium oxysporum f. sp. lycopersici. | the effect of the arbuscular mycorrhizal symbiosis (am) varies in plant cultivars. in the present study, we tested whether wild-type, old and modern tomato cultivars differ in the parameters of the am interaction. moreover, the bioprotective effect of am against the soilborne tomato pathogen fusarium oxysporum f. sp. lycopersici (fol) was tested in the different cultivars. ten tomato cultivars were inoculated with the arbuscular mycorrhizal fungus (amf) glomus mosseae alone or in combination wit ... | 2011 | 21674299 |
| pseudomonas fluorescens f113 mutant with enhanced competitive colonization ability and improved biocontrol activity against fungal root pathogens. | motility is one of the most important traits for efficient rhizosphere colonization by pseudomonas fluorescens f113rif (f113). in this bacterium, motility is a polygenic trait that is repressed by at least three independent pathways, including the gac posttranscriptional system, the wsp chemotaxis-like pathway, and the sadb pathway. here we show that the kinb gene, which encodes a signal transduction protein that together with algb has been implicated in alginate production, participates in swim ... | 2011 | 21685161 |
| the synthetic strigolactone gr24 influences the growth pattern of phytopathogenic fungi. | strigolactones that are released by plant roots to the rhizosphere are involved in both plant symbiosis with arbuscular mycorrhizal fungi and in plant infection by root parasitic plants. in this paper, we describe the response of various phytopathogenic fungi to the synthetic strigolactone gr24. when gr24 was embedded in the growth medium, it inhibited the growth of the root pathogens fusarium oxysporum f. sp. melonis, fusarium solani f. sp. mango, sclerotinia sclerotiorum and macrophomina phase ... | 2011 | 21688170 |
| Different active biomolecules involved in biosynthesis of gold nanoparticles by three fungus species. | In this paper, the intracellular gold nanoparticles were biosynthesized using three fungi including Aureobasidium pullulans (A. pullulans), Fusarium sp. and Fusarium oxysporum (F. oxysporum) after immersion the fungal cells in AuCl4- ions solution. UV-vis and FTIR spectrum, and biochemical compositions analysis of Au nano-fungal cells suggested that active biomolecules of reducing sugar of A. pullulans, and proteins in Fusarium sp. and F. oxysporum were tested positive of providing the function ... | 2011 | 21702362 |
| fusarium oxysporum adh1 has dual fermentative and oxidative functions and is involved in fungal virulence in tomato plants. | an alcohol dehydrogenase gene, adh1, has been identified in the vascular wilt fungus fusarium oxysporum f. sp. lycopersici. reverse transcription polymerase chain reaction (rt-pcr) analysis revealed that adh1 is highly expressed in mycelia grown in potato dextrose liquid medium (pdb) under hypoxic conditions, as compared to mycelia grown under aerobic conditions. one spontaneous allyl alcohol-resistant (ally(r)) mutant exhibited insertion of an incomplete f.oxysporum transposable element, while ... | 2011 | 21704720 |
| selection of antagonistic bacteria isolated from the physalis peruviana rhizosphere against fusarium oxysporum. | aims:ôçé cape gooseberries (physalis peruviana) have become increasingly important in colombia for both domestic consumption and the international export market. vascular wilting caused by fusarium oxysporum is the most damaging disease to p.-áperuviana crops in colombia. the control of this pathogen is mainly carried out by chemical and cultural practices, increasing production costs and generating resistance. therefore, the objectives of this study were to test rhizobacteria isolates from p.-á ... | 2011 | 21714836 |
| characterization of plant-growth-promoting traits of acinetobacter species isolated from rhizosphere of pennisetum glaucum. | a total of 31 acinetobacter isolates were obtained from the rhizosphere of pennisetum glaucum and evaluated for their plant-growth-promoting traits. two isolates, namely acinetobacter sp. pucm1007 and a. baumannii pucm1029, produced indole acetic acid (10-13 microgram/ml). a total of 26 and 27 isolates solubilized phosphates and zinc oxide, respectively. among the mineral-solubilizing strains, a. calcoaceticus pucm1006 solubilized phosphate most efficiently (84 mg/ml), whereas zinc oxide was sol ... | 2011 | 21715961 |
| isolation, identification, and culture optimization of a novel glycinonitrile-hydrolyzing fungus-fusarium oxysporum h3. | microbial transformation of glycinonitrile into glycine by nitrile hydrolase is of considerable interest to green chemistry. a novel fungus with high nitrile hydrolase was newly isolated from soil samples and identified as fusarium oxysporum h3 through 18s ribosomal dna, 28s ribosomal dna, and the internal transcribed spacer sequence analysis, together with morphology characteristics. after primary optimization of culture conditions including ph, temperature, carbon/nitrogen sources, inducers, a ... | 2011 | 21720838 |
| the frp1 f-box gene has different functions in sexuality, pathogenicity and metabolism in three fungal pathogens. | plant-pathogenic fungi employ a variety of infection strategies; as a result, fungi probably rely on different sets of proteins for successful infection. the f-box protein frp1, only present in filamentous fungi belonging to the sordariomycetes, leotiomycetes and dothideomycetes, is required for nonsugar carbon catabolism and pathogenicity in the root-infecting fungus fusarium oxysporum. to assess the role of frp1 in other plant-pathogenic fungi, frp1 deletion mutants were generated in fusarium ... | 2011 | 21722294 |
| insecticidal activity and fungitoxicity of plant extracts and components of horseradish (armoracia rusticana) and garlic (allium sativum). | to avoid environmental pollution and health problems caused by the use of traditional synthetic pesticides, there is a trend to search for naturally occurring toxicants from plants. among the compounds discussed for anti-fungal and insecticidal activity, the natural extracts from garlic and horseradish have attracted considerable attention. the objective of this study is to determine the insecticidal and anti-fungal activity of armoracia rusticana and allium sativum l. extracts against larvae of ... | 2011 | 21726146 |
| spectroscopic and mycological studies of co(ii), ni(ii) and cu(ii) complexes with 4-aminoantipyrine derivative. | complexes of the type [m(l)x(2)], where m=co(ii), ni(ii) and cu(ii), have been synthesized with novel no-donor schiff's base ligand, 1,4-diformylpiperazine bis(4-imino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one) which is obtained by the acid catalyzed condensation of 1,4-diformylpiperazine with 4-aminoantipyrine. the elemental analyses, molar conductance measurements, magnetic susceptibility measurements, ir, uv, nmr, mass and epr studies of the compounds led to the conclusion that the ligand acts ... | 2011 | 21752698 |
| hydroxylation of steroids by fusarium oxysporum, exophiala jeanselmei and ceratocystis paradoxa. | the potential of fusarium oxysporum var. cubense uamh 9013 to perform steroid biotransformations was reinvestigated using single phase and pulse feed conditions. the following natural steroids served as substrates: dehydroepiandrosterone (1), pregnenolone (2), testosterone (3), progesterone (4), cortisone (5), prednisone (6), estrone (7) and sarsasapogenin (8). the results showed the possible presence of c-7 and c-15 hydroxylase enzymes. this hypothesis was explored using three synthetic androst ... | 2011 | 21763336 |
| prevalence of deg nala disease in eastern india and its reproduction in buffaloes by feeding fusarium oxysporum infested rice straw. | to undertake a study on prevalence of deg nala disease in eastern states of india and to reproduce the disease in buffaloes by the fusarium spp., isolated from the affected region. | 2011 | 21771416 |
| diversity analysis of type i ketosynthase in rhizosphere soil of cucumber. | fusarium wilt [fusarium oxysporum (sch1.) f.sp. cucumerinum owen.] is a major soil-borne disease of cucumber worldwide, and can cause huge yield losses. biological control of fusarium wilt of cucumber has received considerable attention. many bacteria, particularly actinomycetes, are known to produce secondary metabolites synthesized by polyketide synthases (pkss) with a diverse range of biological activities. ketosynthase (ks) gene diversity was analyzed in samples which were collected from rhi ... | 2011 | 21780139 |
| expression of an optimized argopecten purpuratus antimicrobial peptide in e. coli and evaluation of the purified recombinant protein by in vitro challenges against important plant fungi. | antimicrobial peptides (amp) have been widely described in several organisms from different kingdoms. we recently designed and evaluated a synthetic version of an amp isolated and characterized from argopecten purpuratus hemocytes. this study describes the generation of a chimaeric gene encoding for ap-s, the use of this construct to transform e. coli strain bl21, and the evaluation of the purified recombinant ap-s (raps) as an antifungal agent. the proposed gene coding for rap-s consists of 93 ... | 2011 | 21787820 |
| effects of surface treatments and application shanks on nematode, pathogen and weed control with 1,3-dichloropropene. | background: preplant fumigation with methyl bromide (mebr) has been used for control of soilborne pests in high-value annual, perennial and nursery crops, but is being phased out. in 2007 and 2008, research trials were conducted to evaluate the effects of surface treatments and two application shanks on pest control with 1,3-dicloropropene (1,3-d) in perennial crop nurseries. results: all 1,3-d treatments controlled nematodes similarly to mebr. application of 1,3-d with virtually impermeable fil ... | 2011 | 21796757 |
| [allelopathy of garlic root exudates]. | by the method of water culture, the root exudates of cangshan garlic and caijiapo garlic were collected to study their allelopathic effects on the seed germination and seedling growth of lettuce, and on the development of pathogens fusarium oxysporum f. sp. cucumerinum and f. oxysporium f. sp. niveum. the root exudates of the two garlic cultivars promoted the lettuce seed germination and seedling growth at low concentrations (0.1 and 0.2 g x ml(-1)), but showed inhibitory effects at high concent ... | 2011 | 21812318 |
| [mixed infection caused by meloidogyne and pathogenic fungi on siraitia grosvenorii]. | to study the mixed infection caused by meloidogyne and pathogenic fungi on siraitia grosvenorii,the result can provide basis for controltion. | 2011 | 21818963 |
| apoptosis-related genes confer resistance to fusarium wilt in transgenic 'lady finger' bananas. | fusarium wilt, caused by fusarium oxysporum f. sp. cubense (foc), is one of the most devastating diseases of banana (musa spp.). apart from resistant cultivars, there are no effective control measures for the disease. we investigated whether the transgenic expression of apoptosis-inhibition-related genes in banana could be used to confer disease resistance. embryogenic cell suspensions of the banana cultivar, 'lady finger', were stably transformed with animal genes that negatively regulate apopt ... | 2011 | 21819535 |
| comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens. | the vascular wilt fungi verticillium dahliae and v. albo-atrum infect over 200 plant species, causing billions of dollars in annual crop losses. the characteristic wilt symptoms are a result of colonization and proliferation of the pathogens in the xylem vessels, which undergo fluctuations in osmolarity. to gain insights into the mechanisms that confer the organisms' pathogenicity and enable them to proliferate in the unique ecological niche of the plant vascular system, we sequenced the genomes ... | 2011 | 21829347 |
| antifungal activity and phytochemical screening of extracts from phoenix dactylifera l. cultivars. | in this study, the rachis extracts of eight date palm phoenix dactylifera l. cultivars were analysed by phytochemical screening and bioautography on fusarium oxysporum f. sp. albedinis (foa). the choice of cultivars was based on their reaction to foa (resistant, tolerant and sensitive). phytochemical screening was realised for flavonoids, tannins, alkaloids and coumarins. antifungal effects were mostly represented by dichloromethanic extracts (seven out of nine inhibition zones). the best result ... | 2011 | 21834627 |
| stable integration and expression of a plant defensin in tomato confers resistance to fusarium wilt. | -á plant defensins are small cysteine-rich peptides which belong to a group of pathogenasis related defense mechanism proteins. the proteins inhibit the growth of a broad range of microbes and are highly stable under extreme environmental stresses. tomato cultivation is affected by fungal disease such as fusarium wilt. in order to overcome fungal damages, transgenic tomato plants expressing the medicago sativa defensin gene msdef 1 under the control of the camv 35s promoter were developed. the f ... | 2010 | 21844692 |
| the arabidopsis thaliana dna-binding protein ahl19 mediates verticillium wilt resistance. | verticillium spp. are destructive soilborne fungal pathogens that cause vascular wilt diseases in a wide range of plant species. verticillium wilts are particularly notorious, and genetic resistance in crop plants is the most favorable means of disease control. in a gain-of-function screen using an activation-tagged arabidopsis mutant collection, we identified four mutants, a1 to a4, which displayed enhanced resistance toward the vascular wilt species verticillium dahliae, v. albo-atrum and v. l ... | 2011 | 21864046 |
| evaluation of luminex xtag fungal analyte-specific reagents for rapid identification of clinically relevant fungi. | invasive fungal infections (ifi) remain a serious threat to immunocompromised hosts. current diagnostic methods, including fungal culture and antigen detection, are slow and often lack specificity. rapid diagnostic tools with increased sensitivity and specificity could improve the care of patients with ifi. recently, luminex molecular diagnostics (toronto, canada) developed 23 analyte-specific reagents (asrs) for the detection of the most common clinically relevant fungi. this study's objective ... | 2011 | 21880976 |
| systemically induced resistance and microbial competitive exclusion: implications on biological control. | the root-knot nematode, meloidogyne incognita, is among the most damaging agricultural pests, particularly to tomato. the mutualistic endophytes fusarium oxysporum strain fo162 (fo162) and rhizobium etli strain g12 (g12) have been shown to systemically induce resistance towards m. incognita. by using triple-split-root tomato plants, spatially-separated but simultaneous inoculation of both endophytes did not lead to additive reductions in m. incognita infection. more importantly, spatially-separa ... | 2011 | 21899390 |
| galleria mellonella as model host for the trans-kingdom pathogen fusarium oxysporum. | fusarium oxysporum, the causal agent of vascular wilt disease, affects a wide range of plant species and can produce disseminated infections in humans. f. oxysporum f. sp. lycopersici isolate fgsc 9935 causes disease both on tomato plants and immunodepressed mice, making it an ideal model for the comparative analysis of fungal virulence on plant and animal hosts. here we tested the ability of fgsc 9935 to cause disease in the greater wax moth galleria mellonella, an invertebrate model host that ... | 2011 | 21907298 |
| GMC oxidoreductase, a highly expressed protein in a potent biocontrol agent Fusarium oxysporum Cong:1-2, is dispensable for biocontrol activity. | A spontaneous non-pathogenic variant (Cong:1-2) derived from Fusarium oxysporum f. sp. conglutinans (Cong: 1-1), a causal agent of cabbage yellows, carries biocontrol activity for cabbage yellows. We found a GMC oxidoreductase (ODX1) among the proteins expressed much more in Cong:1-2 than Cong:1-1 by 2D-DIGE comparison. GMC oxidoreductases have been reported to be involved in biocontrol activity of several plant pathogenic fungi. The gene encoding ODX1 in Cong:1-2 was cloned, and targeted disrup ... | 2011 | 21914969 |
| isolation and characterization of an exopolygalacturonase from fusarium oxysporum f.sp. cubense race 1 and race 4. | fusarium wilt is an economically devastating disease that affects banana production. although cavendish banana cultivars are resistant to fusarium oxysporum f.sp. cubense race 1 (foc1) and maitain banana production after gros michel was destructed by race 1, a new race race 4 (foc4) was found to infect cavendish. | 2011 | 21920035 |
| Arsenic biotransformation by arsenic-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1. | Bioremediation of arsenic (As)-contaminated soil using microorganisms has been a focus of research because it is environment friendly and cost-effective. The As-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were exposed to 50 mg l(-1) of As(V), and the biotransformation of As and the concomitant variance of Eh and pH in the media were studied. Fresh weights of all three isolates increased and then decreased depended on cultivation ... | 2011 | 21920586 |
| synthetic molecular mimics of naturally occurring cyclopentenones exhibit antifungal activity towards pathogenic fungi. | the naturally occurring reactive electrophilic species 12-oxo-phytodienoic acid (12-oxo-pda) is a potent antifungal agent, whereas the plant growth regulator jasmonic acid, which is synthesized from 12-oxo-pda, is ineffective. to address what structural features of the molecule endow it with antifungal activity, we synthesized a series of molecular mimics of 12-oxo-pda varying in the length of the alkyl chain at its c-4 ring position. the octyl analogue (4-octyl cyclopentenone) was the most effe ... | 2011 | 21921102 |
| a highly conserved effector in fusarium oxysporum is required for full virulence on arabidopsis. | secreted-in-xylem (six) proteins of the vascular wilt pathogen fusarium oxysporum f. sp. lycopersici are secreted during infection of tomato and function in virulence or avirulence. f. oxysporum formae speciales have specific host ranges but the roles of six proteins in diverse hosts are unknown. we identified homologs of f. oxysporum f. sp. lycopersici six1, six4, six8, and six9 in the genome of arabidopsis infecting isolate fo5176. a six4 homolog (termed fo5176-six4) differed from that of f. o ... | 2011 | 21942452 |
| Cloning and characterization of a bifunctional glycosyl hydrolase from an antagonistic Pseudomonas putida strain P3(4). | A fluorescent pseudomonad strain P3(4) showing chitinolysis on chitinase detection agar and antagonism against Fusarium oxysporum f.sp dianthi causing vascular wilt of carnation was isolated from pea rhizosphere soil. PCR primers specific for glycosyl hydrolase family 5 (GH5) of Pseudomonas putida isolate KT2440 amplified a 947 bp fragment of the GH5 gene from P3(4). Cloning of this gene into Escherichia coli M15 using an expression vector pQE-30UA and screening on chitin and chitosan detection ... | 2011 | 21953214 |
| purification and characterization of an extracellular 24 kda chitobiosidase from the mycoparasitic fungus trichoderma saturnisporum. | a trichoderma saturnisporum hamill isolate gitx-panog (c) exhibiting strong chitinolytic and antifungal activity against fusarium oxysporum f.sp. dianthi, the causal agent of vascular wilt in carnation was used to purify extracellular chitobiosidase using czapek-dox broth amended with the fungal mycelium as the carbon source. the protein was purified by precipitation with ammonium sulphate, followed by deae-cellulose anion-exchange and sephacryl s-200 high resolution gel filtration chromatograph ... | 2011 | 21953631 |
| The antifungal, cytotoxic, antitermite and insecticidal activities of Zizyphus jujube. | Plants are very useful, self-generating machines, producing a variety of useful bioactive products. Keeping in view this idea, the crude methanolic extract and various fractions of Zizyphus jujuba were screened for antifungal, cytotoxic, antitermite and insecticidal activities. Low activity was shown by the crude methanolic extract (12%), n-hexane (9%), chloroform (20%) and ethyl acetate (14%) fraction against Penicillium notatum. Low activity was shown by the n-hexane fraction against Aspergill ... | 2011 | 21959810 |
| molecular identification of two vegetative compatibility groups of fusarium oxysporum f. sp. cepae. | fusarium oxysporum f. sp. cepae (focep), which causes basal rot of onions, consists of seven vegetative compatibility groups (vcgs 0420 to 0426) and several single-member vcgs (smvs). focep populations in south africa and colorado each comprise one main vcg, namely vcg 0425 and 0421, respectively. the aim of this study was to develop sequence-characterized amplified region (scar) markers for the identification of vcgs 0425 and 0421, using 79 previously characterized f. oxysporum isolates. a seco ... | 2011 | 21970568 |
| Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113. | Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) inc ... | 2011 | 21972146 |
| Three new compounds from soil actinomycete Streptomyces albospinus 15-4-2. | Three new compounds, 2-methyl-2,5,6-bornantriol (1), 4,4'-(3-hydroxypropane-1,1-diyl)diphenol (2), and 7-(4-methoxybenzyl)-4,5,6,7-tetrahydro-1,3-oxazepine-5,6-diol (3), were isolated from the fermentation broth of the soil actinomycete Streptomyces albospinus 15-4-2. Their structures were completely elucidated using the combination of 1D, 2D NMR techniques (COSY, HMQC, HMBC, and ROESY), and HR-ESI-MS analysis. None of the compounds 1-3 showed any inhibitory effect on Fusarium oxysporum f.sp. cu ... | 2011 | 21972804 |
| identification of limiting factors for the optimum growth of fusarium oxysporum in liquid medium. | fusarium oxysporum is a highly ubiquitous species that infects a wide range of hosts causing various diseases such as vascular wilts, yellows, rots, and damping-off. despite the immense economic significance of this phytopathogen, few workers have reported growth studies in this genus in submerged culture. in the present study, several parameters such as change in media ph, biomass, pattern of substrate utilization, viability of the fungal cells, and protein content were observed over a period o ... | 2011 | 21976815 |
| genetic diversity of fusarium oxysporum f.sp. cubense isolates (foc) of india by inter simple sequence repeats (issr) analysis. | to find out the genetic diversity of indian foc isolates of banana, a total of 107 isolates of fusarium which includes 98 foc isolates obtained from different banana growing regions of india and seven foc isolates belong to all known vcgs obtained from australia and two non-pathogenic fusarium oxysporum (npfo) isolates were subjected to issr analysis. in the initial screening of issr primers, out of 34, 10 primers which generated more polymorphic bands were selected for further analysis. the phy ... | 2011 | 21983913 |
| phylogeny and pathogenicity of fusarium oxysporum isolates from cottonseed imported from australia into california for dairy cattle feed. | a unique biotype of the fusarium wilt pathogen, fusarium oxysporum schlecht. f.sp. vasinfectum (atk) sny. & hans., found in australia in 1993 is favored by neutral or alkaline heavy soils and does not require plant parasitic nematodes to cause disease. this makes it a threat to 4-6 million acres of usa upland cotton ( gossypium hirsutum l.) that is grown on heavy alkaline soil and currently is not affected by fusarium wilt. in 2001-2002, several shiploads of live cottonseed were imported into ca ... | 2011 | 22004096 |
| [Identification of soil-borne fungi using Fourier transform infrared spectroscopy]. | Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium monili forme, Fusarium semitectum, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from ... | 2011 | 22007392 |
| effects of (14)c-labelled precursor feeding on production of beauvericin, enniatins h, i, and mk1688 by fusarium oxysporum kfcc11363p. | the effects of (14)c-labelled precursor feeding on the production of cyclic hexadepsipeptides were investigated by the mycelium of f. oxysporum kfcc11363p producing beauvericin along with enniatins h, i, and mk1688. most (14)c-phenylalanine and (14)c-valine were incorporated easily into the biosynthetic pathway of (14)c-labelled beauvericin in vivo by the mycelium. however, only a small amount of (14)c-labelled enniatins could be detected by feeding of (14)c-valine. when l-valine was fed as a pr ... | 2011 | 22019403 |
| structural characterization of the o-chain polysaccharide from an environmentally beneficial bacterium pseudomonas chlororaphis subsp. aureofaciens strain m71. | pseudomonas chlororaphis subsp. aureofaciens strain m71 was isolated from the root of a tomato plant and it was able to control in vivo fusarium oxysporum f. sp. radicis-lycopersici responsible for the tomato crown and root rot. recently, strain m71 was evaluated even for its efficacy in controlling seiridium cardinale, the causal agent of bark canker of common cypress (cupressus sempervirens l.). strain m71 ability to persist on the tomato rhizosphere and on the aerial part of cypress plants co ... | 2011 | 22036123 |
| gene expression specificity of the mussel antifungal mytimycin (mytm). | we previously reported the nucleotide sequences and diversity of mytimycin (mytm) from the mediterranean mussel, mytilus galloprovincialis. using real-time pcr (q-pcr), we observed that the mytm gene was mainly expressed in circulating hemocytes and to a less extent in the mantle. in vivo challenge with bacteria or with the yeast, candida albicans, did not increase the expression as measured by q-pcr in hemocytes. by contrast, injection of the filamentous fungus, fusarium oxysporum, induced a su ... | 2012 | 22037382 |
| the presence of fungi on contact electrical stimulation electrodes and ultrasound transducers in physiotherapy clinics. | to evaluate the presence of fungi on contact electrodes and ultrasound transducers from physiotherapy clinics. | 2011 | 22051582 |
| antimicrobial peptaibols from trichoderma pseudokoningii induce programmed cell death in plant fungal pathogens. | antibiosis is one of the widespread strategies used by trichoderma spp. against plant fungal pathogens, the mechanism of which, however, remains poorly understood. peptaibols are a large family of antimicrobial peptides produced by trichoderma spp. our previous study showed that trichokonins, a type of peptaibol from trichoderma pseudokoningii smf2, exhibited antibiotic activities against plant fungal pathogens. in this study, we first demonstrated that trichokonin vi (tk vi) induced extensive a ... | 2012 | 22053006 |
| characterization of the gene encoding pisatin demethylase (fopda1) in fusarium oxysporum. | the pea pathogen fusarium oxysporum f. sp. pisi is able to detoxify pisatin produced as a defense response by pea, and the gene encoding this detoxification mechanism, fopda1, was 82% identical to the cytochrome p450 pisatin demethylase pda1 gene in nectria haematococca. a survey of f. oxysporum f. sp. pisi isolates demonstrated that, as in n. haematococca, the pda gene of f. oxysporum f. sp. pisi is generally located on a small chromosome. in n. haematococca, pda1 is in a cluster of pea pathoge ... | 2011 | 22066900 |
| longitudinal melanonychia caused by fusarium oxysporum in immunocompetent patient. | 2011 | 22074386 | |
| identification and functional characterization of indole-3-acetamide-mediated iaa biosynthesis in plant-associated fusarium species. | the plant hormone indole-3-acetic acid (iaa) can be synthesized from tryptophan via the intermediate indole-3-acetamide (iam). the two genes, iaam (encoding tryptophan monooxygenase) and iaah (encoding indole-3-acetamide hydrolase) that constitute the iam pathway have been described in plant-associated bacteria. we have identified putative homologs of the bacterial iaam and iaah genes in four fusarium species -fusarium proliferatum, fusarium verticillioides, fusarium fujikuroi, and fusarium oxys ... | 2011 | 22079545 |
| A new antifungal coumarin from Clausena excavata. | A new ?-lactone coumarin, named as excavarin-A, showing antifungal activity was isolated from the leaves of Clausena excavata by bioassay guided fractionation method. The structure was elucidated by spectroscopic data analysis and identified as 7((2E)-4(4,5-dihydro-3-methylene-2-oxo-5-furanyl)-3-methylbut-2-enyloxy) coumarin. Minimum inhibitory concentration (MIC) was determined against fifteen fungal strains pathogenic against plants and human. The least MIC was recorded against the human patho ... | 2012 | 22088496 |
| the effect of galleria mellonella apolipophorin iii on yeasts and filamentous fungi. | galleria mellonella apolipophorin iii (apolp-iii) has been implicated in the innate immune response against bacterial infections. the protein binds components of bacterial cell wall and inhibits growth of selected gram-positive and gram-negative bacteria. interaction of apolp-iii with fungal β-1,3-glucan suggests antifungal properties of the protein. in the present study, the effect of apolp-iii on the growth, metabolic activity and cell surface characteristics of selected yeasts and filamentous ... | 2012 | 22100292 |
| Characterization of five fungal endophytes producing Cajaninstilbene acid isolated from pigeon pea [Cajanus cajan (L.) Millsp]. | Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L) among the endophytes tested after incubation for five days. Both morphological ... | 2011 | 22102911 |
| a resource for the in silico identification of fungal polyketide synthases from predicted fungal proteomes. | the goal of this study was to develop a tool specifically designed to identify iterative polyketide synthases (ipkss) from predicted fungal proteomes. a fungi-based pks prediction model, specifically for fungal ipkss, was developed using profile hidden markov models (phmms) based on two essential ipks domains, the β-ketoacyl synthase (ks) domain and acyltransferase (at) domain, derived from fungal ipkss. this fungi-based pks prediction model was initially tested on the well-annotated proteome of ... | 2011 | 22112245 |
| constitutive expression, purification and characterization of a phosphoglucomutase from fusarium oxysporum. | the phosphoglucomutase gene from a wild type fusarium oxysporum strain (f3), was homologously expressed, under the control of the constitutive promoter of gpda of aspergillus nidulans. the transformant produced elevated levels of phosphoglucomutase activity compared to the wild type, a fact that facilitated the subsequent purification procedure. the enzyme (fopgm) was purified to homogeneity applying three anion exchange and one gel filtration chromatography steps. the native enzyme revealed a m ... | 2011 | 22112903 |
| [the effect of the formulation on the shelf-life of biopesticides based on colombian isolates of trichoderma koningiopsis th003 and trichoderma asperellum th034.] | background: four biopesticide prototypes formulated as dispersible granules and dry powders based on 2 colombian isolates of trichoderma koningiopsis (th003) and t. asperellum (th034) were developed. these microorganisms have antagonist activity against fusarium oxysporum f. sp. lycopersici and rhizoctonia solani with a reduction in incidence of between 70 and 100% in tomato crops and potato crops, respectively. aim: to determine the effect of the formulation on the shelf-life of 4 biopesticides ... | 2011 | 22116019 |
| studies on fungal cultural filtrates against adult culex quinquefasciatus (diptera: culicidae) a vector of filariasis. | entomopathogenic fungi have significant potential to control mosquito population. the culture filtrates of fusarium oxysporum, lagenidium giganteum, trichophyton ajelloi, and culicinomyces clavisporus were evaluated against adults of cx. quinquefasciatus. the culture filtrates were obtained by filtering the broth through whatman-1 filter paper. these culture filtrates of c. clavisporus have been found significantly pathogenic with lc(50)-2.5, lc(90)-7.24, and lc(99)-8.7 ml, respectively, after e ... | 2011 | 22132305 |
| Cloning of novel cellulases from cellulolytic fungi: Heterologous expression of a family 5 glycoside hydrolase from Trametes versicolor in Pichia pastoris. | Total cDNA isolated from cellulolytic fungi cultured in cellulose was examined for the presence of sequences encoding for endoglucanases. Novel sequences encoding for glycoside hydrolases (GHs) were identified in Fusarium oxysporum, Ganoderma applanatum and Trametes versicolor. The cDNA encoding for partial sequences of GH family 61 cellulases from F. oxysporum and G. applanatum shares 58 and 68% identity with endoglucanases from Glomerella graminicola and Laccaria bicolor, respectively. A new G ... | 2011 | 22142722 |
| direct hydride shift mechanism and stereoselectivity of p450(nor) confirmed by qm/mm calculations. | nitric oxide reductase (p450(nor)) found in fusarium oxysporum catalyzes the reduction of nitric oxide to n(2)o in a multistep process. the reducing agent, nadh, is bound in the distal pocket of the enzyme, and direct hydride transfer occurs from nadh to the nitric oxide bound heme enzyme, forming intermediate i. here we studied the possibility of hydride transfer from nadh to both the nitrogen and oxygen of the heme-bound nitric oxide, using quantum chemical and combined quantum mechanics/mol ... | 2011 | 22148861 |
| ERK1/2 and AKT pathway were activated during (3R,6R)-Bassiatin (1)-induced apoptosis in MCF-7 cells. | (3R,6R)-bassiatin(1) was isolated from the endogenous fungus Fusarium oxysporum J8-1-2. Our previous studies show that (3R,6R)-bassiatin(1) is a novel antiestrogen agent which is cytotoxic to estrogen receptor (ER)-positive breast cancer MCF-7 cells via cell cycle arrest and induction of apoptosis. (3R,6R)-Bassiatin(1) could suppress the protein and mRNA expression of estrogen receptor (ERa) and estrogen responsive gene cyclinD1 and PR leading to cell cycle arrest and apoptosis. In the present ... | 2011 | 22150072 |
| antifungal metabolites from the roots of diospyros virginiana by overpressure layer chromatography. | a preparative overpressure layer chromatography (oplc) method was successfully used for the separation of two new natural compounds, 4-hydroxy-5,6-dimethoxynaphthalene-2-carbaldehyde (1) and 12,13-didehydro-20,29-dihydrobetulin (2) together with nine known compounds, including 7-methyljuglone (3), diospyrin (4), isodiospyrin (5), shinanolone (6), lupeol (7), betulin (8), betulinic acid (9), betulinaldehyde (10), and ursolic acid (11) from the acetone extract of the roots of diospyros virginiana. ... | 2011 | 22162171 |
| Antifungal saponins from bulbs of white onion, Allium cepa L. | Three saponins, named ceposide A, ceposide B, and ceposide C were isolated from the bulbs of white onion, Allium cepa L. Elucidation of their structure was carried out by comprehensive spectroscopic analyses, including 2D NMR spectroscopy and mass spectrometry, and chemical evidences. The structures of the compounds were identified as (25R)-furost-5(6)-en-1ß,3ß,22a,26-tetraol 1-O-ß-d-xylopyranosyl 26-O-a-d-rhamnoyranosyl-(1?2)-O-ß-d-galactopyranoside (ceposide A), (25R)-furost-5(6)-en-1ß,3ß,22a, ... | 2011 | 22169018 |
| a comparative in silico analysis on frequency and distribution of microsatellites in coding regions of three formae speciales of fusarium oxysporum and development of est-ssr markers for polymorphism studies. | fusarium oxysporum is a ubiquitous species complex of soil-borne plant pathogens comprising of many different formae speciales, each characterized by a high degree of host specificity. in the present investigation, we surveyed microsatellites in the available express sequence tags and transcript sequences of three formae speciales of f. oxysporum viz. melonis (fom), cucumerium (foc), and lycopersici (fol). the relative abundance and density of microsatellites were higher in fom when compared wit ... | 2011 | 22171944 |
| synthesis of pyrazolo[3,4-b]pyridines under microwave irradiation in multi-component reactions and their antitumor and antimicrobial activities - part 1. | an efficient one-pot synthesis in multi-component system (mrcs) for the preparation of pyrazolo[3,4-b]pyridine derivatives from the reaction of 5-amino-1-phenyl-3-(pyridin-3-yl)-1h-pyrazole with 4-anisaldehyde and p-substituted β-ketonitriles or with pyruvic acid and some aromatic aldehydes in acetic acid medium. the reactions were carried out by two different techniques, conventional heating and microwave irradiation. these compounds were screened for their antibacterial activity against gram-p ... | 2011 | 22178093 |
| expression of rice thaumatin-like protein gene in transgenic banana plants enhances resistance to fusarium wilt. | the possibility of controlling fusarium wilt-caused by fusarium oxysporum sp. cubensec (race 4)-was investigated by genetic engineering of banana plants for constitutive expression of rice thaumatin-like protein (tlp) gene. transgene was introduced to cauliflower-like bodies' cluster, induced from meristemic parts of male inflorescences, using particle bombardment with plasmid carrying a rice tlp gene driving by the camv 35s promoter. hygromycin b was used as the selection reagent. the presence ... | 2011 | 22183565 |