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locus-specific microsatellite markers for the fungal chickpea pathogen didymella rabiei (anamorph) ascochyta rabiei. 200011091341
historical and contemporary multilocus population structure of ascochyta rabiei (teleomorph: didymella rabiei) in the pacific northwest of the united states.the historical and contemporary population genetic structure of the chickpea ascochyta blight pathogen, ascochyta rabiei (teleomorph: didymella rabiei), was determined in the us pacific northwest (pnw) using 17 putative aflp loci, four genetically characterized, sequence-tagged microsatellite loci (stms) and the mating type locus (mat). a single multilocus genotype of a. rabiei (mat1-1) was detected in 1983, which represented the first recorded appearance of ascochyta blight of chickpea in the p ...200414717888
the chickpea, summer cropping, and a new model for pulse domestication in the ancient near east.the widely accepted models describing the emergence of domesticated grain crops from their wild type ancestors are mostly based upon selection (conscious or unconscious) of major features related either to seed dispersal (nonbrittle ear, indehiscent pod) or free germination (nondormant seeds, soft seed coat). based on the breeding systems (self-pollination) and dominance relations between the allelomorphs of seed dispersal mode and seed dormancy, it was postulated that establishment of the domes ...200314737827
mapping quantitative trait loci in chickpea associated with time to flowering and resistance to didymella rabiei the causal agent of ascochyta blight.drought is the major constraint to chickpea (cicer arietinum l.) productivity worldwide. utilizing early-flowering genotypes and advancing sowing from spring to autumn have been suggested as strategies for drought avoidance. however, ascochyta blight (causal agent: didymella rabiei (kov.) v. arx.) is a major limitation for chickpea winter cultivation. most efforts to introgress resistance to the pathogen into kabuli germplasm resulted in relatively late flowering germplasm. with the aim to explo ...200617016689
cloning and characterization of the mating type (mat) locus from ascochyta rabiei (teleomorph: didymella rabiei) and a mat phylogeny of legume-associated ascochyta spp.degenerate primers designed to correspond to conserved regions of the high mobility group (hmg) protein encoded by the mat1-2 gene of cochliobolus heterostrophus, cochliobolus sativus, and alternaria alternata were used to amplify the portion of the sequence corresponding to the hmg box motif from ascochyta rabiei (teleomorph: didymella rabiei). a combination of tail and inverse pcr extended the mat1-2 sequence in both directions, then primers designed to mat1-2 flanking dna were used to amplify ...200312781674
production of didymella rabiei pseudothecia and dispersal of ascospores in a mediterranean climate.abstract temperature and wetness conditions required for development and maturation of didymella rabiei pseudothecia were determined in a series of experiments conducted in controlled-environmental conditions. initial stages of pseudothecium formation occurred at temperatures ranging from 5 to 15 degrees c. incubation at low temperatures was essential for subsequent pseudothecium maturation. this requirement was satisfied for chickpea stem segments incubated at 5 or 10 degrees c for three consec ...200518943358
rational management of didymella rabiei in chickpea by integration of genotype resistance and postinfection application of fungicides.abstract various aspects of the integration of genotype resistance and chemical control of ascochyta blight (caused by didymella rabiei) in chickpea were examined in field experiments from 1993 to 1999 and in greenhouse experiments. four commercially available chickpea cultivars representing a range of resistance to d. rabiei were used. the efficacy of chemical control in a highly susceptible cultivar was significantly (p < 0.01) related to the conduciveness of the environment to the pathogen. a ...200018944504
phenology of didymella rabiei development on chickpea debris under field conditions in spain.abstract the development of didymella rabiei on debris of naturally infected chickpea was investigated in four chickpea-growing areas with different climatic conditions in spain during 1987 to 1992. d. rabiei extensively colonized chickpea debris and formed pseudothecia and pycnidia. differentiation of pseudothecial initials occurred regularly across experimental locations by november, 1 month after placement of debris on the soil. ascospore maturation occurred mainly from late january to late m ...199818944877
different ecological affinities and aggressiveness patterns among didymella rabiei isolates from sympatric domesticated chickpea and wild cicer judaicum.domesticated chickpea (cicer arietinum) and its wild relative c. judaicum grow in sympatric distribution in israel and both are susceptible to ascochyta blight caused by didymella rabiei. c. arietinum was grown for millennia in drier and hotter levantine spring conditions while c. judaicum grows in the wetter and milder winters. accordingly, it is possible that d. rabiei isolates originated from c. arietinum are adjusted to the less favorable spring conditions. here, 60 isolates from both origin ...200818943229
ecological genetic divergence of the fungal pathogen didymella rabiei on sympatric wild and domesticated cicer spp. (chickpea).for millennia, chickpea (cicer arietinum) has been grown in the levant sympatrically with wild cicer species. chickpea is traditionally spring-sown, while its wild relatives germinate in the autumn and develop in the winter. it has been hypothesized that the human-directed shift of domesticated chickpea to summer production was an attempt to escape the devastating ascochyta disease caused by didymella rabiei. we estimated genetic divergence between d. rabiei isolates sampled from wild cicer juda ...201019897759
biometric analyses of the inheritance of resistance to didymella rabiei in chickpea.abstract historically, the response of chickpea (cicer arietinum l.) to didymella rabiei (causal agent of ascochyta blight) has been mainly related to as complete resistance and it was commonly assayed with qualitative (nonparametric) scales. two reciprocal populations, derived from intra-specific crosses between a moderately resistant late flowering israeli cultivar and a highly susceptible early flowering indian accession, were tested at f(3) and f(4) generations in 1998 and 1999, respectively ...200218942955
a bac/bibac-based physical map of chickpea, cicer arietinum l.chickpea (cicer arietinum l.) is the third most important pulse crop worldwide. despite its importance, relatively little is known about its genome. the availability of a genome-wide physical map allows rapid fine mapping of qtl, development of high-density genome maps, and sequencing of the entire genome. however, no such a physical map has been developed in chickpea.201020849583
differences between ascospores and conidia of didymella rabiei in spore germination and infection of chickpea.abstract studies were performed to compare the germination and infection of ascospores and conidia of didymella rabiei under different temperature and moisture conditions. germination of ascospores and conidia on cover glasses coated with water agar began after 2 h, with maximum germination (>95%) occurring in 6 h at 20 degrees c. no germination occurred at 0 and 35 degrees c. ascospores germinated more rapidly than conidia at all temperatures. germination declined rapidly as the water potential ...200718943721
genetic analyses and conservation of qtl for ascochyta blight resistance in chickpea (cicer arietinum l.).ascochyta blight (ab) caused by ascochyta rabiei (teleomorph, didymella rabiei) pass. lab. is an important fungal disease of chickpea worldwide. only moderate sources of resistance are available within the cultivated species and we hypothesized that the available sources may carry different genes for resistance, which could be pyramided to improve field resistance to ab. four divergent moderately resistant cultivars cdc frontier, cdc luna, cdc corinne, and amit were each crossed to a highly susc ...200919517090
mapping the mating type locus of ascochyta rabiei, the causal agent of ascochyta blight of chickpea.summary a genome linkage map was developed for ascochyta rabiei (pass.) labrousse, (teleomorph) didymella rabiei (kovachevski), an important pathogen causing ascochyta blight in chickpea (cicer arietinum l.). the map was constructed using 96 progeny generated from a single pseudothecium produced from a cross between a usa mat-2 isolate and an australian mat-1 isolate. the map comprised 126 molecular markers of which 69 were random amplified polymorphic dna (rapd) markers, 46 were amplified fragm ...200320569397
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