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tryptophan transport in neurospora crassa: a tryptophan-binding protein released by cold osmotic shock.osmotic shock treatment of germinated conidia of neurospora reduced the capacity for tryptophan transport in these cells approximately 90% without an appreciable loss of cell viability. tryptophan-binding proteins and alkaline phosphatase were consistently released into the osmotic shock fluid by this treatment. four lines of evidence suggest that the binding protein may be related to the tryptophan transport system. (i) it appears to be located on or near the cell surface. (ii) a decreased capa ...19705474881
feedback inhibition of l-glutamine d-fructose 6-phosphate amidotransferase by uridine diphosphate n-acetylglucosamine in neurospora crassa.the enzyme, l-glutamine d-fructose 6-phosphate amidotransferase (ec 2.6.1.16) of neurospora crassa, which catalyzes the formation of glucosamine 6-phosphate was shown to be subject to feedback inhibition by uridine diphosphate n-acetyl-d-glucosamine (udp-glcnac). the conclusion is based on the following observations. udp-glcnac, the direct precursor of chitin, did not accumulate in the cell even when its utilization for the synthesis of cell wall chitin was interrupted by the antibiotic polyoxin ...19705474877
genetically induced subcellular mislocation of neurospora mitochondrial malate dehydrogenase.among 60 ultraviolet-induced missense mutations of the structural genes that code for mitochondrial malate dehydrogenase (m-mdh, ec 1.1.1.37) of neurospora crassa, two enzyme phenotypes are observed. in a previously described class (c-mutants), m-mdh is malfunctional because of an abnormal conformation induced by association with mitochondria. we describe here a second class (k-mutants) in which the enzyme is malfunctional because of an altered subcellular location. thus, although both classes c ...19705272317
cysteine toxicity in neurospora crassa: comparison of counteraction by sulphur amino acids and iron. 19704252565
sugar transport in neurospora crassa. ii. a second glucose transport system. 19705492960
a genetic analysis of non-disjunction and mitotic recombination in neurospora crassa. 19705488985
control of recombination within the nitrate-2 locus of neurospora crassa: an unlinked dominant gene which reduces prototroph yields. 19705477713
nucleotide 'maps' of digests of deoxyribonucleic acid.various digests of (32)p-labelled dna were examined by two-dimensional ionophoresis on cellulose acetate and deae-cellulose paper. the products from digestion with pancreatic deoxyribonuclease and neurospora crassa endonuclease were qualitatively closely similar, but very complex, and were used to investigate the mapping behaviour of nucleotides in various ionophoretic systems. ionophoresis on deae-cellulose paper in triethylamine carbonate, ph 9.7, followed by ionophoresis in the second dimensi ...19705476726
pyrimidine-specific carbamyl phosphate synthetase in neurospora crassa.two carbamyl phosphate synthetases, the first an arginine-synthetic enzyme (cps(arg)) and the second a pyrimidine-synthetic enzyme (cps(pyr)), are shown to be present in neurospora. the two enzymes can be separated on the basis of size and are distinguished by several different properties. both cps(pyr) and cps(arg) have substrate requirements of adenosine triphosphate, hco(3) (-), and l-glutamine, although nh(4) (+) in high concentration will partially replace glutamine. cps(pyr) activity can b ...19705432004
an assessment of in vivo mitochondrial protein synthesis in neurospora crassa. 19705470823
purification and properties of homoserine dehydrogenase from neurospora crassa. 19705500942
the mechanism of uptake of cobalt ions by neurospora crassa.uptake of co(2+) by 3-day-old mycelia of neurospora crassa involves cell-surface binding as well as transport into the intracellular space. the surface binding is rapid and accounts for 30-40% of the total co(2+) uptake. transport of co(2+) occurs at a rate of 40mug/h per 100mg dry wt. surface binding and overall uptake show different temperature dependence. metabolic inhibitors such as azide, dinitrophenol and fluoride depress transport of co(2+). the overall uptake of co(2+) exhibits a high k( ...19705472176
methods for the study of nuclear and cytoplasmic variation in respiratory activity of neurospora crassa, and the discovery of three new genes. 19705488472
the beta-galactosidase system of neurospora crassa. ii. extracellular nature of the ph 4.2 enzyme. 19705433578
the beta-galactosidase system of neurospora crassa. i. purification and properties of the ph 4.2 enzyme. 19705433577
genetic and metabolic controls for sulfate metabolism in neurospora crassa: isolation and study of chromate-resistant and sulfate transport-negative mutants.mutants of neurospora resistant to chromate were selected and all were found to map at a single genetic locus designated as cys-13. the chromate-resistant mutants grow at a wild-type rate on minimal media but are partially deficient in the transport of inorganic sulfate, especially during the conidial stage. an unlinked mutant, cys-14, is sensitive to chromate but transports sulfate during the mycelial stage at only 25% of the wild-type rate; cys-14 also grows at a fully wild-type rate on minima ...19705429722
mutagenicity of aflatoxins in neurospora crassa. 19705424724
the gemini-xi s-4 spaceflight-radiation interaction experiment. 3. comparison of the spectra of recessive lethal mutations at specific loci in the ad-3 region in neurospora crassa. 19705421854
glucose-6-phosphate dehydrogenase and neurospora morphology.in addition to the neurospora crassa mutant col-2, mutations in two other unlinked genes, balloon and frost, lead to distinct morphological growth and abnormal glucose-6-phosphate dehydrogenases. the glucose-6-phosphate dehydrogenases from these strains have increased thermolabilities, altered kinetic parameters, and distinctive electrofocusing patterns. the altered enzyme segregates with each morphological phenotype in crosses to wild type, and there is a correlation between the severity of the ...19705271177
interconvertible forms of glycogen phosphorylase in neurospora crassa.glycogen phosphorylase in extracts of n. crassa mycelia has two interconvertible forms: one active (a form) and the other inactive (b form) in the absence of 5'-amp. the conversion of the b to the a form requires atp-mg(2+) and proceeds at higher rate in the presence of 3',5'-cyclic amp.19704317917
the pyruvate dehydrogenase complex from the mitochondrial fraction of neurospora crassa. 19704317491
effect of cycloheximide on the synthesis and modification of ribosomal rna in neurospora crassa. 19705421964
complications affecting the distinction between mendelian and maternally inheritd characters in neurospora crassa. 19705440053
inhibition by d-glutamate of growth and glutamate dehydrogenase activity of neurospora crassa. 19705476895
location of aryl sulfatase in conidia and young mycelia of neurospora crassa.aryl sulfatase (arylsulfate sulfohydrolase, ec 3.1.6.1) was found to have multiple locations in neurospora conidia. some enzyme activity remained in the supernatant when a spore suspension was centrifuged or filtered. part of the cell-bound activity could be detected by adding the assay ingredients to a suspension of intact spores (patent enzyme), and additional activity was only detectable when the spores were first treated to destroy their permeability barriers (cryptic enzyme). such treatment ...197016559101
a suppressor of the heterokaryon-incompatibility associated with mating type in neurospora crassa. 19705512565
regulation of recombination at the his-3 locus in neurospora crassa. 19705496221
allosteric interaction of d-glutamate with the glutamate dehydrogenases of neurospora crassa. 19704398827
developmental regulation of amino acid transport in neurospora crassa.conidia of neurospora crassa exhibit an ability to transport various amino acids against a concentration gradient. the conidial transport system has previously been characterized in terms of kinetics, competitions, and genetic control. this study describes the development of a new and highly active transport capability which is elaborated during the early stages of development but prior to evident germination. it has been named "postconidial" transport activity and represents as much as 20-fold ...19705489433
altered fatty acid distribution in mutants of neurospora crassa.morphological mutants of neurospora with decreased levels of reduced nicotinamide adenine dinucleotide phosphate (nadph) and reduced nicotinamide ad enine dinucleotide (nadh) contained only 20% as much of a polyunsaturated fatty acid (linolenic acid) as the wild type in both the phospholipid and neutral lipid fractions. there was an excellent correlation between linolenic acid levels and morphological appearance as a function of total nadph content, but no correlation with nadh content. the lino ...19704395199
copurification of pyrimidine-specific carbamyl phosphate synthetase and aspartate transcarbamylase of neurospora crassa. 19705472708
a nutritional method for the isolation of morphological mutants of neurospora crassa. 19705483778
new crisp genes and crisp-modifiers in neurospora crassa. 19705512362
enzyme degradation of conidial wall during germination of neurospora crassa. 19705502332
histidine-mediated control of tryptophan biosynthetic enzymes in neurospora crassa.the formation of the five tryptophan biosynthetic enzymes of neurospora crassa was shown to be derepressed in histidine-starved cells. this histidine-mediated derepression was not due to a lowered intracellular concentration of tryptophan in these cells. furthermore, histidine-mediated derepression of tryptophan enzymes was found to be coordinate and not subject to reversal by tryptophan of either exogenous or biosynthetic origin. the synthesis of tryptophan enzymes also was found to be coordina ...19705473920
alterations of the respiratory system of neurospora crassa by the mi-1 mutation.the respiratory mechanism of the cytochrome-defective mi-1 mutant of neurospora crassa was partially characterized and compared with that of the wild type. both systems of electron transport were linked to oxidative phosphorylation and showed similar characteristics with respect to substrates and components. the system operating in the cytochrome-deficient mi-1 mutant showed no characteristic alpha absorption bands of cytochromes a or b and was very unstable in vitro. the possibility that this s ...19705473916
mechanism of action of the antifugal agent polyoxin d.the antibiotic polyoxin d was shown to inhibit the incorporation of (14)c-glucosamine into cell wall chitin in neurospora crassa at levels which were comparable with those required for inhibition of fungal growth. at the same time, the antibiotic increased the accumulation of a nucleotide, which was identified as uridine diphosphate (udp)-n-acetylglucosamine, indicating inhibition of chitin synthesis. chitin synthetase (udp-n-acetylglucosamine: chitin n-acetylglucosaminyl transferase, ec 2.4.1.1 ...19705473886
[electrophoretic analysis of carotenogenic proteins of neurospora crassa]. 19705478625
amino acid transport in neurospora crassa. 3. acidic amino acid transport. 19705456980
genetically mixed perithecia and somatic recombination in a pseudowild type strain of neurospora crassa. 19705516682
topography of the ad-3 region of neurospora crassa. 19705516678
genetic and biochemical studies of distinct sulfate permease species in different developmental stages of neurospora crassa. 19705446339
mutagenic action of nitrous acid on two transversions in the histidine-3 locus of neurospora crassa. 19705424718
arginaseless neurospora: genetics, physiology, and polyamine synthesis.four arginaseless mutants of neurospora crassa have been isolated. all carry mutations which lie at a single locus, aga, on linkage group viir. a study of aga strains shows the arginase reaction to be the major, perhaps the only, route of arginine consumption in neurospora other than protein synthesis. ornithine-delta-transaminase, the second enzyme of the arginine catabolic pathway, is present and normally inducible by arginine in aga strains, and ornithine transcarbamylase, an enzyme of argini ...19705419257
purification and properties of dihydroxy acid dehydratase from soluble and mitochondrial fractions of neurospora crassa. 19704986563
characterization of neurospora crassa mitochondria prepared with a grind-mill. 19704392865
carcinogenic and noncarcinogenic polycyclic hydrocarbons in neurospora crassa and chinese hamster cells: their photodynamic effects. 19704193459
sugar transport in neurospora crassa. 19705438359
genetic fine structure of the albino (al) region of neurospora crassa. 19705480750
deficiency of dihydroxy acid dehydratase in the mitochondria of the iv-i mutants of neurospora crassa. 19704987443
amino acid transport in neurospora crassa. ii. properties of a basic amino acid transport system. 19705445671
inheritance of growth rate in neurospora crassa: crosses between previously selected lines. 19705487923
isolation of neurospora crassa endonuclease specific for single-stranded dna. 19705438327
role of enzymes in growth and morphology of neurospora crassa: cell-wall-bound enzymes and their possible role in branching.the cell wall of neurospora crassa contains bound enzymes that can digest its structural polymers. these enzymes are not present at the same levels at all stages of growth. the levels of these autolytic enzymes vary and generally show some relationship to the process of branching. these enzymes were removed from the cell wall by beta-mercaptoethanol extraction and were tested for activity against isolated cell wall fractions. such studies, as well as autolytic studies, showed that enzymes acting ...19705438053
conversion of histidine to hercynine by neurospora crassa.cell-free extracts of neurospora crassa mycelium catalyze the methylation of l-histidine to form hercynine (histidine betaine). the partially methylated compounds, alpha-n-methyl-l-histidine and alpha-n,n-dimethyl-l-histidine, are also methylated by the same enzyme preparation to form hercynine. all three methylation reactions are stimulated by the addition of s-adenosylmethionine.19705438052
correlation between reduced nicotinamide adenine dinucleotide phosphate levels and morphological changes in neurospora crassa.a colonial mutant of neurospora crassa, previously shown to be altered in the structure of glucose-6-p dehydrogenase [a reduced nicotinamide adenine dinucleotide phosphate (nadph) producing reaction], contained only 40% as much nadph in extracts as did the wild type. a partial revertant strain, when grown at 23 c, had the same total nadph content as the wild type, but, at 34 c, had lower levels of nadph as well as a colonial morphology. a revertant with complete wild-type morphology had wild-typ ...19704392398
isoenzymes and allosteric inhibition of neurospora crassa 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase. 19705416121
catalytic facilitation in vitro by two multienyzme complexes from neurospora crassa. 19704312868
localization of the beta-glucosidases in neurospora crassa.the beta-glucosidases (ec 3.2.1.21) of neurospora crassa were studied with respect to their location in conidia and young mycelia. aryl-beta-glucosidase of conidia was nearly equally divided between extracellular and bound activity. bound aryl-beta-glucosidase was almost all available to substrate. an induction procedure was used to maximize both beta-glucosidases in 4 to 6-hr cells. aryl-beta-glucosidase was entirely bound but still mostly (90%) detectable, whereas cellobiase was mostly interna ...19705413819
characterization of icr-177-induced ad-3 mutants of neurospora crassa. 19705413676
variation of recombination frequency in neurospora crassa following temperature changes prior to and during meiosis and evidence for a premeiotic sensitive stage. 19705519984
[cell nuclei histones in neurospora crassa]. 19705519212
[effect of 8-azaadenine on the metabolism of phosphorus compounds in neurospora crassa mutants auxotrophic with respect to adenine]. 19705515433
analysis of a case of mutagen specificity in neurospora crassa. 3. fractionated treatment with diepoxybutane (deb). 19705497287
selection and characterisation of mutants lacking arginase in neurospora crassa. 19705495901
conserved mrna from the conidia of neurospora crassa. 19705491723
analysis of a case of mutagen specificity in neurospora crassa. ii. interaction between treatments with diepoxybutane (deb) and ultraviolet light. 19705488083
genetic effects of n-methyl-n'-nitro-n-nitrosoguanidine in neurospora crassa. 19705480466
[correlation of accumulation of certain fractions of inorganic polyphosphates and rna in neurospora crassa ad 28-610]. 19705470181
nucleoside uptake during the germination of neurospora crassa conidia. 19705453517
[effect of antimetabolites on a neurospora crassa mutant resistant to 5-azarotic acid]. 19705433931
[enzyme patterns during conidiogenesis in neurospora crassa]. 19705429635
[selection of actidion-resistant mutants in neurospora crassa and their genetical and biochemical analysis]. 19705415255
a gene controlling the early development of protoperithecium in neurospora crassa. 19704938066
catabolite-controlled regulation of glutamate dehydrogenases of neurospora crassa. 19704391972
enzymatic properties of l-kynurenine 3-hydroxylase (ec 1.14.1.2) in neurospora crassa. 19704391570
mitochondrial peptide chain elongation factors from neurospora crassa.two complementary peptide chain elongation factors (g and t) have been isolated from a mitochondrial 100,000 g supernatant. both factors are specific for 70 s ribosomes and can be crossed with t and g factors from e. coli.197011945348
[electrophoretic and immunochemical analysis of endocellular soluble proteins of wild type neurospora crassa compared with those of one of its morphological mutants]. 19704985467
organization of polyaromatic biosynthetic enzymes in a variety of photosynthetic organisms.sucrose density gradient centrifugation was used to estimate the molecular weights and determine possible physical aggregation of the enzymes catalyzing steps 2 to 6 in pre-chorismic acid polyaromatic biosynthesis in anabaena variabilis, chlamydomonas reinhardi, euglena gracilis, nicotiana tabacum, and physcomitrella patens. in a. variabilis, the five enzymes are separable. similar results were obtained for p. patens, n. tabacum, and c. reinhardi extracts, except that dehydroshikimate reductase ...19704992369
aminoacylation of escherichia coli valine transfer ribonucleic acid by neurospora crassa phenylalanyl transfer ribonucleic acid synthetase in tris(hydroxymethyl)aminomethane hydrochloric acid and potassium cacodylate buffers. effect of salts, dimethyl sulfoxide, ethanol, and 2-mercaptoethanol. 19704909562
biochemical and histochemical localization of invertase in neurospora crassa during conidial germination and hyphal growth.the intracellular localization of neurospora invertase, an enzyme partially secreted and partially retained by neurospora at the cell periphery, was investigated. a cell wall fraction was isolated, to which 24% of the cell-bound invertase was firmly attached. a sensitive osmiophilic stain for invertase was developed and used in conjunction with the technique of indirect immunofluorescence to follow the pattern of invertase localization during the development of neurospora from the germination of ...19704192564
purification of neurospora crassa endonuclease-resistant dna-rna hybrid. 19705463194
major constituents of the conidial wall of neurospora crassa. 19705494280
mutants of neurospora crassa, escherichia coli and salmonella typhimurium specifically inhibited by carbon dioxide. 19705500023
the specific interaction of chromate with the dual sulfate permease systems of neurospora crassa. 19715550165
invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a neurospora mutant and a component of molybdenum-enzymes.an active neurospora-like assimilatory nadph-nitrate reductase (ec 1.6.6.2), which can be formed in vitro by incubation of extracts of nitrate-induced neurospora crassa mutant nit-1 with extracts of (a) certain other nonallelic nitrate reductase mutants, (b) uninduced wild type, or (c) xanthine oxidizing and liver aldehyde-oxidase systems was also formed by combination of the nit-1 extract with other acid-treated enzymes known to contain molybdenum. these molybdenum enzymes included (a) nitrogen ...19714399835
the amino acid composition and some properties of the nad + -specific glutamate dehydrogenase from neurospora crassa. 19714399934
nh2-terminal methionine in nascent peptides from neurospora crassa. 19714925709
mitochondrial rna polymerase from neurospora crassa. 19714933004
transcription of mitochondrial dna in vitro from neurospora crassa. 19714938619
fusidic acid resistance of mitochondrial g factor from neurospora crassa. 19714942120
a structural gene for neurospora crassa isocitrate lyase. 19714996780
studies with purple adenine mutants in neurospora crassa. vi. the effects of differences in genetic background on ad-3a x ad-3b crosses. 19715127402
a survey of the interaction of calcium ions with mitochondria from different tissues and species.a survey was made of the capacity of mitochondria isolated from a number of different tissues and species to accumulate ca(2+) from the suspending medium during electron transport. the species examined included the rat, mouse, rabbit, hamster, guinea pig, cow, chicken, turtle, blowfly, yeast and neurospora crassa. the tissues examined included vertebrate liver, kidney, brain, heart, spleen, thyroid and adrenal cortex, and the flight muscle of the blowfly. the mitochondria from all vertebrate tis ...19715129264
isoprenoid phenol and quinone precursors of ubiguinones and dihydroubiguinones (ubiguinones (h 2 )) in fungi.1. ten moulds and two yeasts were analysed for the presence of 2-polyprenylphenols, 2-polyprenyl(h(2))phenols, 6-methoxy-2-polyprenylphenols, 6-methoxy-2-polyprenyl(h(2))phenols, 6-methoxy-2-polyprenyl-1,4-benzoquinones, 6-methoxy-2-polyprenyl(h(2))-1,4-benzoquinones, 5-demethoxyubiquinones, 5-demethoxyubiquinones(h(2)), ubiquinones and ubiquinones(h(2)). 2. the organisms were found to be of three types: (a) those that contained only ubiquinones (aspergillus fumigatus and penicillium brevi-compa ...19715166547
a cytoplasmic protein from neurospora crassa resembling membrane proteins. 19715166638
a paracrystalline inclusion in neurospora crassa. induction by ethidium and acridine, isolation, and characterization.a paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type neurospora crassa after growth in either ethidium or euflavine. this paracrystal has been isolated and partially characterized. it appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the ph and ionic strength. when aggregated, the polypeptide forms oligomers which are arranged end-to-end into fib ...19714329522
a nuclease from neurospora crassa conidia specific for single-stranded nucleic acids. 19714269245
neurospora crassa ribosomes: effects of bound peptidyl-trna on dissociation into subunits and on phenylalanine polymerization at low mg 2+ concentration. 19714257627
in vitro mitochondrial complementation in neurospora crassa. 19715116521
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