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transformation of aspergillus oryzae using the a. niger pyrg gene.a transformation system for aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrg) was developed. transformation frequencies of up to 16 transformants per microgram of dna were obtained with the vector pab4-1, which carries the pyrg gene of a. niger. southern blotting analysis showed that vector dna sequences were integrated into the chromosomal dna, in various copy numbers and presumably at different sites. efficient cotransformation of an unselectable gene was also sh ...19873481025
molybdenum cofactor: a compound in the in vitro activation of both nitrate reductase and trimethylamine-n-oxide reductase activities in escherichia coli k12.nitrate reductase (nitrite: (acceptor) oxidoreductase, ec 1.7.99.4) and trimethylamine n-oxide reductase (nadh : trimethylamine-n-oxide oxidoreductase, ec 1.6.6.9) activities were reconstituted by incubation of the association factor fa (the putative product of the chlb gene) with the soluble extract of the chlb mutant grown anaerobically in the presence of trimethylamine n-oxide. when soluble extracts of the chlb mutant grown on 10 mm sodium tungstate, a molybdenum competitor, were used in comp ...19863524687
activation in vitro of respiratory nitrate reductase of escherichia coli k12 grown in the presence of tungstate. involvement of molybdenum cofactor.the chlorate-resistant (chlr) mutants are pleiotropically defective in molybdoenzyme activity. the inactive derivative of the molybdoenzyme, respiratory nitrate reductase, present in the cell-free extract of a chlb mutant, can be activated by the addition of protein fa, the probable active product of the chlb locus. protein fa addition, however, cannot bring about the activation if 10 mm sodium tungstate is included in the culture medium for the chlb strain. the inclusion of a heat-treated prepa ...19863525161
further characterization of trimethylamine n-oxide reductase from escherichia coli, a molybdoprotein.escherichia coli trimethylamine n-oxide (tmao) reductase i, the major enzyme among inducible tmao reductases, was purified to homogeneity by an improved method including heat treatment, ammonium sulfate precipitation, and chromatographies on bio-gel a-1.5m, deae-cellulose, and reactive blue-agarose. the molecular weight was estimated by gel filtration to be approximately 200,000. a single subunit peptide with a molecular weight of 95,000 was found by sodium dodecyl sulfate-polyacrylamide gel ele ...19863528139
biosynthesis of thiamin. different biosynthetic routes of the thiazole moiety of thiamin in aerobic organisms and anaerobic organisms.the nitrogen atom of glycine was incorporated into the thiazole moiety of thiamin in the aerobic microorganisms bacillus subtilis, pseudomonas putida, saccharomyces cerevisiae, mucor racemosus, neurospora crassa, and emericella nidulans. it was not incorporated in the case of the facultative anaerobic microorganisms escherichia coli and enterobacter aerogenes, which, however, did incorporate the nitrogen atom of tyrosine. these results show that aerobic microorganisms and facultative anaerobic m ...19873566774
complete amino-acid sequence of a functional unit from a molluscan hemocyanin (helix pomatia).from the beta c-hemocyanin (beta c-hc) of the vineyard snail, helix pomatia, the functional unit d (mr approximately equal to 50,000-55,000) was isolated by limited proteolysis and gel chromatography. a small quantity of functional unit d was obtained intact, but the major part in the form of two peptides (mr approximately equal to 43,000 and 10,000, respectively) connected by a disulfide bridge. after reduction and carboxymethylation, these were separated from each other and cleaved by conventi ...19873620107
a long polypyrimidine/polypurine tract induces an altered dna conformation on the 3' coding region of the adjacent myosin heavy chain gene.a long (147 base pairs), natural a.t rich polypyrimidine/polypurine tract has been found 55 base pairs downstream of a chicken embryonic myosin heavy chain (mhc) gene. analysis at the nucleotide level of nicks induced by s1 and neurospora crassa nucleases indicate that this long interrupted polypyrimidine/polypurine tract exists in an alternate dna structure in vitro at ph 4.5 and ph 7.5 in both supercoiled and linear plasmid dna. the polypyrimidine/polypurine tract induces this alternate struct ...19873671071
extraction and purification of molybdenum cofactor from milk xanthine oxidase.molybdenum cofactor (mocofactor) is extracted efficiently, free of impurities and in high concentrations, by acid treatment of xanthine oxidase and subsequent incubation of the precipitate with phosphate buffer containing edta, molybdate and oxygen. it is suggested that cofactor is bound to the enzyme via hydrophobic forces as well as via an oxygen-sensitive mechanism. upon extraction, the capability to complement the apo nitrate reductase of neurospora crassa nit-1 can be conserved only in the ...19873691496
molybdopterin cofactor from methanobacterium formicicum formate dehydrogenase.the molybdopterin cofactor from the formate dehydrogenase of methanobacterium formicicum was studied. the cofactor was released by guanidine denaturation of homogeneous enzyme, which also released greater than 80% of the molybdenum present in the enzyme. the anoxically isolated cofactor was nonfluorescent, but after exposure to air it fluoresced with spectra similar to those of described molybdopterin cofactors. aerobic release from acid-denatured formate dehydrogenase in the presence of i2 and ...19863700335
purification and properties of nitrate reductase from mitsuokella multiacidus.nitrate reductase of mitsuokella multiacidus (formerly bacteroides multiacidus) was solublized from the membrane fraction with 1% sodium deoxycholate and purified 40-fold by immunoaffinity chromatography on the antibody-affi-gel 10 column. the preparation showed a major band (86% of total protein) with enzyme activity and a minor band on polyacrylamide gel after disc electrophoresis in the presence of 0.1% triton x-100. sodium dodecyl sulfate-polyacrylamide gel electrophoresis gave a major band, ...19863711052
identification of molybdoproteins in clostridium pasteurianum.cells of clostridium pasteurianum whose n source is switched from nh3 to n2 accumulate large amounts of molybdenum beginning 1.5 h before the detection of nitrogenase activity. anaerobic multiphasic gel electrophoresis and anion-exchange chromatography were used to identify the molybdoproteins and molybdenum-containing components present in n2-fixing cells. in addition to molybdate, six distinct 99mo-labeled species were detected, i.e., a membrane fragment, the mofe protein of nitrogenase, forma ...19853857223
involvement of a particular species of beta-tubulin (beta 3) in conidial development in aspergillus nidulans.strains of aspergillus containing the bena22 mutation are resistant to benomyl for vegetative growth but do not produce conidia. to test whether conidiation involved an additional benomyl-sensitive tubulin (i.e., was mediated by a tubulin other than the tubulins coded for by the bena locus), a collection of mutants was produced that formed conidia in the presence of benomyl, i.e., were conidiation-resistant (cr-) mutants. we analyzed the tubulins of these cr- mutants using two-dimensional gel el ...19853897246
identification and functional analysis of beta-tubulin genes by site specific integrative transformation in aspergillus nidulans.we have cloned two different beta-tubulin sequences from the filamentous fungus aspergillus nidulans. each was used in the construction of transforming plasmids that carry the pyr4 gene of neurospora crassa. we used these plasmids to transform a pyrg-strain of aspergillus to uridine prototrophy. both plasmids were shown to integrate site specifically into the homologous chromosomal sequences. we then used transformant strains in genetic crosses to demonstrate that one of the cloned beta-tubulin ...19853897247
cloning and characterization of the three enzyme structural genes qutb, qutc and qute from the quinic acid utilization gene cluster in aspergillus nidulans.heterologous dna probes from the quinic acid gene cluster (qa) in neurospora crassa (schweizer 1981) have been used to isolate the corresponding gene cluster (qut) from aspergillus nidulans cloned in a phage lambda vector. n. crassa probes for each of the three enzyme structural genes in the cluster have been used to identify the corresponding genes within the a. nidulans cloned dna. the three genes are in the same relative sequence [dehydrogenase (1), qa-3 = qutb; dehydratase (3), qa-4 = qutc; ...19853916726
specific inhibition of mitochondrial protein synthesis influences the amount of complex i in mitochondria of rat liver and neurospora crassa directly.specific inhibition of mitochondrial protein synthesis reduces the oxidation rate of nadh-linked substrates in rat liver as well as in neurospora crassa mitochondria. the present study shows that this is due to the fact that inhibition of mitochondrial protein synthesis leads to a decrease of the concentration of active complex i. therefore, these results demonstrate that at least one of the genes for the subunits of complex i is localized on mitochondrial dna.19853934002
the mitochondrial genome of the fission yeast schizosaccharomyces pombe. the cytochrome b gene has an intron closely related to the first two introns in the saccharomyces cerevisiae cox1 gene.the dna sequence of the cob region of the schizosaccharomyces pombe mitochondrial dna has been determined. the cytochrome b structural gene is interrupted by an intron of 2526 base-pairs, which has an open reading frame of 2421 base-pairs in phase with the upstream exon. the position of the intron differs from those found in the cob genes of saccharomyces cerevisiae, aspergillus nidulans or neurospora crassa. the sch. pombe cob intron has the potential of assuming an rna secondary structure almo ...19854046021
sequence of the n-terminal formic acid fragment and location of the n-ethylmaleimide-binding site of the phosphate transport protein from beef heart mitochondria.the n-terminal formic acid fragment (fa1) of the n-[3h]ethylmaleimide-labeled and carboxymethylated bovine mitochondrial phosphate transport protein (ptpn*cm) has been purified and completely sequenced: nh2-ala-val-glu-glu-gln-tyr-ser-cys-asp-tyr10-gly-ser-gly-arg-phe- phe-ile-leu-cys- gly20-leu-gly-gly-ile-ile-ser-cys-gly-thr-thr30-his-thr -ala-leu-val-pro-leu-asp- -leu-val40-lys-cys(n-[3h]ethylmaleimide)-arg-met-gln-val-asp- cooh. by thermolysin digestion of fa1 and high-performance liquid chr ...19854066697
amino-acid sequence of nadp-specific glutamate dehydrogenase of neurospora crassa.a tentative primary structure of the nadp-specific glutamate dehydrogenase [l-glutamate: nadp oxidoreductase (deaminating), ec 1.4.1.4] from neurospora crassa has been determined. the proposed sequence contains 452 amino-acid residues in each of the identical subunits of the hexameric enzyme. comparison of the sequence with that of the bovine liver enzyme reveals considerable homology in the amino-terminal portion of the chain, including the vicinity of the reactive lysine, with only shorter str ...19744155068
isolation and characterization of chromatin from neurospora crassa.different preparations of chromatin isolated from mycelia of neurospora crassa were analyzed for dna-associated rna and proteins. the uv absorption spectra, the ultrastructure of chromatin, and the amino acid composition of the acid-extractable proteins were studied. the protein:dna ratios range from 1.5 to 2.8; the rna:dna ratios range from 0.5 to 1.24. uv absorption shows a macimum at 259 mmicro and a minimum at 238-239 mmicro. the e280/e260 ranges from 0.59 to 0.70. electron microscopy reveal ...19694186412
biochemical and histochemical localization of invertase in neurospora crassa during conidial germination and hyphal growth.the intracellular localization of neurospora invertase, an enzyme partially secreted and partially retained by neurospora at the cell periphery, was investigated. a cell wall fraction was isolated, to which 24% of the cell-bound invertase was firmly attached. a sensitive osmiophilic stain for invertase was developed and used in conjunction with the technique of indirect immunofluorescence to follow the pattern of invertase localization during the development of neurospora from the germination of ...19704192564
[griseocarnin, an antifungal antibiotic isolated from streptoverticillium criseocarneum (ia-7527)].we have been studying the antagonistic activity of the streptoverticillium griseocarneum, strain ia-7527. it produces a strong antifungal substance which we named griseocarnin. its antimicrobial power to neurospora crassa, rhodotorula, rubra, hansenula suaveolens and candida albicans deserves to be exceled. the isolated substance was compared with other antifungal antibiotics presenting close similarity with the heptaens ascosin and pa-150. the production, extraction and purification methods as ...19744220132
inhibition of tryptophan synthetase by indoleacrylic acid.indoleacrylic acid (5 x 10(-4)m in the growth medium) inhibits the growth of mycelia of neurospora crassa and causes the cells to accumulate indoleglycerol phosphate. measurement of kinetic parameters of partially purified tryptophan synthetase in the presence of indoleacrylic acid showed the following patterns of inhibition: in reaction 1, the physiological reaction, indoleglycerol phosphate + serine [formula: see text] tryptophan + glyceraldehyde-3-phosphate (unusual with respect to indoleglyc ...19724259663
regulation of exocellular proteases in neurospora crassa: induction and repression of enzyme synthesis.neurospora crassa strain 74a grown on vogel's medium containing bovine serum albumin (bsa) as principal carbon source secretes proteolytic enzymes which appear in the culture filtrate. low concentrations of sucrose (0.1%) are necessary for growth from conidia, as conidia will not germinate on bsa alone. once growth is initiated, however, protease production begins and at 5 to 6 hr growth and enzyme production are parallel. higher concentrations of sucrose (0.5-2%) repress protease synthesis. oth ...19724260559
genetic and enzymatic analysis of a gene controlling uv sensitivity in neurospora crassa. 19724261527
mutation induction in neurospora crassa incubated in mice and rats. 19724261732
[study on the conidial differentiation of neurospora crassa. ii. immunological comparison of conidiogenic wild-type antigenic structures with those of 2 aconidial mutants]. 19724262114
enzymic properties of a mutant tryptophan synthase from neurospora crassa. 19724262962
acetohydroxy acid synthetase with a ph optimum of 7.5 from neurospora crassa mitochondria: characterization and partial purification.an acetohydroxy acid synthetase (aas) has been found associated with the mitochondrial fraction of wild-type neurospora crassa. it has a ph optimum of 7.5 and is presumed to be homologous to the ph 8.0 aas that synthesizes the valine and isoleucine precursors in bacteria and yeast. the enzyme was characterized and purified 30- to 60-fold. the aas activity of intact mitochondria requires thiamine pyrophosphate (tpp), mn(2+) or mg(2+), and flavine adenine dinucleotide (fad), and is sensitive to en ...19724263405
interaction of genes controlling ultraviolet sensitivity in neurospora crassa.two independently segregating ultraviolet (uv) sensitivity genes in neurospora crassa interact synergistically resulting in uv sensitivity approximately twice that expected based on an evaluation of the sensitivities of the individual mutants. the mutant genes singly and together reduce photoreactivation (pr) in vivo although a pr enzyme is produced which exhibits normal activity in vitro.19724263406
uncoupling of the recbc atpase from dnase by dna crosslinked with psoralen.exonucleolytic cleavage of dna by the recbc dnase is accompained by a dna-dependent atp hydrolysis that ceases when the dna that has been digested to a limit. on the other hand, dna that has been crosslinked by 4,5',8-trimethylpsoralen in the presence of 360-nm light remains an effective cofactor in the atpase reaction, but is resistant to digestion by the enzyme. psoralentreated dna is degraded by pancreatic dnase, micrococcal nuclease, and escherichia coli b restriction enzyme, but not by neur ...19724263506
an inhibitor of ribonucleic acid polymerase from neurospora crassa. 19724263858
interactions of phenylalanyl transfer ribonucleic acid synthetase of neurospora crassa with valyl transfer ribonucleic acid of escherichia coli. 19724264131
[neurospora crassa pyruvate dehydrogenase: interconversion due to phosphorylation and dephosphorylation]. 19724265372
the isolation of histone from neurospora crassa. 19734266136
distinct kynureninase and hydroxykynureninase activities in microorganisms: occurrence and properties of a single physiologically discrete enzyme in yeast.(i) saccharomyces cerevisiae grown in the presence of 1.0 mm l-tryptophan slowly excreted fluorescent material that was chromatographically identifiable as 3-hydroxyanthranilate but did not excrete detectable amounts of anthranilate nor rapidly deplete the medium of l-tryptophan. under similar growth conditions, neurospora crassa rapidly excretes anthranilate and rapidly depletes the medium of l-tryptophan. (ii) chromatographic analysis of crude extracts from yeast revealed a single kynureninase ...19734266242
dissociation of mitochondrial ribosomes of neurospora crassa by a bacterial dissociation factor. 19734266973
an inhibitory factor affecting ribonuclease activity in neurospora crassa. 19734267553
the ribonuclease activities of the single-strand-specific nucleases of neurospora crassa. 19734267583
particle weights of active ribosomal subunits from neurospora crassa. 19734268077
adenylate cyclase activity in neurospora crassa. 3. modulation by glucagon and insulin. 19734268860
a nuclease from neurospora crassa conidia specific for single-stranded nucleic acids. 19714269245
in vitro formation of nitrate reductase using extracts of the nitrate reductase mutant of neurospora crassa, nit-1, and rhodospirillum rubrum.in vitro formation of reduced nicotinamide adenine dinucleotide phosphate (nadph)-nitrate reductase (nadph: nitrate oxido-reductase, ec 1.6.6.2) has been attained by using extracts of the nitrate reductase mutant of neurospora crassa, nit-1, and extracts of either photosynthetically or heterotrophically grown rhodospirillum rubrum, which contribute the constitutive component. the in vitro formation of nadph-nitrate reductase is characterized by the conversion of the flavin adenine dinucleotide ( ...19734270447
effects of yeast proteinase and its inhibitor on the inactivation of tryptophan synthase from saccharomyces cerevisiae and neurospora crassa. 19734272540
purine and pyrimidine biosynthesis in neurospora crassa and human skin fibroblasts. alteration by ribosides and ribotides of allopurinol and oxipurinol. 19744275559
metabolic products of microorganisms. 132. uptake of iron by neurospora crassa. 3. iron transport studies with ferrichrome-type compounds. 19744275989
properties of repressible alkaline phosphates from wild type and a wall-less mutant of neurospora crassa. 19744276965
immunological difference of mitochondrial and cytoplasmic ribosomes of neurospora crassa. 19744277526
isocitrate lyase from neurospora crassa. i. purification, kinetic mechanism, and interaction with inhibitors. 19744278771
isocitrate lyase from neurospora crassa. ii. composition, quaternary structure, c-terminus, and active-site modification. 19744278772
translation products in vitro of mitochondrial messenger rna from neurospora crassa. 19744279820
the modifying effects of strain and age on the mutagenic specificity of ultraviolet light in neurospora crassa.two derivatives of k3/17 ad-3a 38701; inos 37401 of neurospora crassa are described which show opposite specific reversional responses to uv. both derivatives carry the same two auxotrophic alleles and appear to differ only in a single gene which influences the pattern of mutagen specificity. the differences between the derivatives only develop after the cultures have been aged for two to four weeks. various possible explanations are considered.19744282639
mutagenic and recombinogenic activities of the food additive furylfuramide in eurkaryotes.the mutagenic and recombinogenic activities of furylfuramide, an antimicrobial food additive, were tested in a two-component heterokaryon of neurospora crassa and a diploid strain of saccharomyces cerevisiae, respectively. the results show that furylfuramide is genetically active in both eukaryotic organisms; it induces mutations in neurospora crassa and mitotic crossing over in saccharomyces cerevisiae.19744283384
feedback inhibition of glutamine synthetase of neurospora crassa by nicotinamide-adenine dinucleotide. 19684300703
effects of inorganic pyrophosphate on neurospora crassa phenylalanine transfer ribonucleic acid ligase in heterologous aminoacylation reactions. 19694309130
a paracrystalline inclusion in neurospora crassa. induction by ethidium and acridine, isolation, and characterization.a paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type neurospora crassa after growth in either ethidium or euflavine. this paracrystal has been isolated and partially characterized. it appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the ph and ionic strength. when aggregated, the polypeptide forms oligomers which are arranged end-to-end into fib ...19714329522
an exonuclease of neurospora crassa specific for single-stranded nucleic acids. 19724334920
heterologous charging of neurospora crassa phenylalanine trna by escherichia coli valyl-trna synthetase. 19724337752
neurospora crassa pyruvate dehydrogenase: interconversion by phosphorylation and dephosphorylation. 19724352021
infectious dna from herpes simplex virus: infectivity of double-stranded and single-stranded molecules.the infectious units in native and alkalidenatured preparations of dna of herpes simplex virus were characterized with respect to their sensitivity to neurospora crassa endonuclease, their sedimentation properties in high-salt, neutral sucrose gradients, and their sensitivity to hydrodynamic shearing forces. infectious molecules in native preparations were resistant to n. crassa endonuclease, sedimented at 56 s, and were highly sensitive to shearing forces. after alkaline denaturation, infectiou ...19734357884
neurospora crassa and humicola lanuginosa cytochromes c: more homology in the heme region. 19744363050
the amino acid composition of the subunit of the nadp-specific glutamate dehydrogenase from neurospora crassa. 19694390421
invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a neurospora mutant and a component of molybdenum-enzymes.an active neurospora-like assimilatory nadph-nitrate reductase (ec 1.6.6.2), which can be formed in vitro by incubation of extracts of nitrate-induced neurospora crassa mutant nit-1 with extracts of (a) certain other nonallelic nitrate reductase mutants, (b) uninduced wild type, or (c) xanthine oxidizing and liver aldehyde-oxidase systems was also formed by combination of the nit-1 extract with other acid-treated enzymes known to contain molybdenum. these molybdenum enzymes included (a) nitrogen ...19714399835
the amino acid composition and some properties of the nad + -specific glutamate dehydrogenase from neurospora crassa. 19714399934
protein fluorescence of nicotinamide nucleotide-dependent dehydrogenases.1. the decrease in the protein fluorescence (f) of neurospora crassa glutamate dehydrogenase is linearly related to the increase in the fraction of the coenzyme sites occupied by nadph (alpha) at ph6.35. under these conditions nadph causes this enzyme to dissociate to monomers. 2. there is a non-linear relationship of f to alpha for nadh binding to give the alcohol dehydrogenase-nadh-isobutyramide complex, the l-glycerol 3-phosphate dehydrogenase-nadh complex and the bovine glutamate dehydrogena ...19724404769
comparative regulation of isoenzymic 3-deoxy-d-arabino-heptulosonate 7-phosphate synthetases in microorganisms.the independent control of regulatory isoenzymes by different metabolites constitutes one well-known pattern of control in branched metabolic pathways. this pattern was previously found to be widely distributed in the aromatic amino acid pathway of microorganisms in the case of the first enzyme of the sequence, 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthetase. the comparative stability of the isoenzymes as well as the effect of aromatic amino acids in the growth medium upon the leve ...19684867215
the pathways of thiamine biosynthesis. some factors controlling synthesis of the pyrimidine moiety by thiamine auxotrophs of neurospora crassa. 19684875319
studies on the aminoacylation of valine- and alanine-specific transfer rna of escherichia coli by aminoacyl transfer rna synthetases from neurospora crassa and e. coli. 19694883911
effects of tris and dimethylsulfoxide on the aminoacylation of escherichia coli valine transfer rna by neurospora crassa phenylalanyl transfer rna synthetase. 19694890606
puromycin sensitivity of ribosomal label after incorporation of 14c-labelled amino acids into isolated mitochondria from neurospora crassa. 19694899931
regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms.1. aspergillus nidulans, neurospora crassa and escherichia coli were grown on media containing a range of concentrations of nitrate, or ammonia, or urea, or l-glutamate, or l-glutamine as the sole source of nitrogen and the glutamate dehydrogenate and glutamine synthetase of the cells measured. 2. aspergillus, neurospora and escherichia coli cells, grown on l-glutamate or on high concentrations of ammonia or on high concentrations of urea, possessed low glutamate dehydrogenase activity compared ...19694901826
codon recognition by enzymatically mischarged valine transfer ribonucleic acid.direct evidence for the adaptor hypothesis has been obtained by examining the codon recognition of a purified escherichia coli valine transfer ribonucleic acid which was enzymatically mischarged with phenylalanine labeled with carbn-14 by reaction with purified phenylalanyl-transfer ribonucleic acid synthetase from neurospora crassa. the mischarged transfer ribonucleic acid recognized the valine codons but failed to recognize the phenylalanine codon when tested in trinucleotide-directed ribosoma ...19694902680
mitochondrial and cytoplasmic ribosomes from neurospora crassa: characterization of their subunits. 19694903818
aminoacylation of escherichia coli valine transfer ribonucleic acid by neurospora crassa phenylalanyl transfer ribonucleic acid synthetase in tris(hydroxymethyl)aminomethane hydrochloric acid and potassium cacodylate buffers. effect of salts, dimethyl sulfoxide, ethanol, and 2-mercaptoethanol. 19704909562
nh2-terminal methionine in nascent peptides from neurospora crassa. 19714925709
mitochondrial rna polymerase from neurospora crassa. 19714933004
transcription of mitochondrial dna in vitro from neurospora crassa. 19714938619
fusidic acid resistance of mitochondrial g factor from neurospora crassa. 19714942120
existence of two phenylalanyl-srna synthetases in neurospora crassa. 19654955766
ribosomes and ribosomal protein from neurospora crassa. i. physical, chemical, and immunochemical properties.ribosomes from neurospora crassa, initially characterized by ultracentrifugal and immunochemical analyses, have been used to prepare ribosomal protein for physical, chemical, and immunochemical study. the acrylamide gel disc electrophoretic profiles of neurospora ribosomal protein exhibit a degree of heterogeneity comparable to what has been observed in other systems. only by chemical modification or by aggregation of the protein do alterations in the profile become apparent. disulfide-bond form ...19674962303
ribosomes and ribosomal proteins from neurospora crassa. ii. ribosomal proteins in different wild-type strains and during various stages of development. 19674965202
[proteins from neurospora crassa. 1. immunochemical analysis of the cytoplasmic proteins from neurospora crassa and various mutants]. 19684973222
[proteins from neurospora crassa. 3. agar gel and immunoelectrophoretic analysis of enzymes and isoenzymes in various neurospora crassa strains]. 19684973224
the facile isolation of a structural phospholipoprotein from hydrogenomon as facilis and neurospora crassa.the paper describes a new and gentle procedure for isolating "structural" phospholipoproteins from organisms and the first such isolation from a procaryotic microbe, hydrogenomonas facilis. the amino acid composition of its protein moiety resembles that of "structural protein" from other sources. although disc gel electrophoresis has shown the protein to be heterogeneous, this is attributed to aggregation.19694978742
organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.ultracentrifugation in sucrose density gradients was employed to estimate the molecular weights and to determine possible physical aggregation of the five enzymes catalyzing steps two to six in the prechorismic acid portion of the polyaromatic synthetic pathway in six species of bacteria: escherichia coli, salmonella typhimurium, aerobacter aerogenes, bacillus subtilis, pseudomonas aeruginosa, and streptomyces coelicolor. the five enzymes were not aggregated in extracts of any of the species exa ...19694979440
[electrophoretic and immunochemical analysis of endocellular soluble proteins of wild type neurospora crassa compared with those of one of its morphological mutants]. 19704985467
organization of polyaromatic biosynthetic enzymes in a variety of photosynthetic organisms.sucrose density gradient centrifugation was used to estimate the molecular weights and determine possible physical aggregation of the enzymes catalyzing steps 2 to 6 in pre-chorismic acid polyaromatic biosynthesis in anabaena variabilis, chlamydomonas reinhardi, euglena gracilis, nicotiana tabacum, and physcomitrella patens. in a. variabilis, the five enzymes are separable. similar results were obtained for p. patens, n. tabacum, and c. reinhardi extracts, except that dehydroshikimate reductase ...19704992369
a structural gene for neurospora crassa isocitrate lyase. 19714996780
studies with purple adenine mutants in neurospora crassa. vi. the effects of differences in genetic background on ad-3a x ad-3b crosses. 19715127402
a survey of the interaction of calcium ions with mitochondria from different tissues and species.a survey was made of the capacity of mitochondria isolated from a number of different tissues and species to accumulate ca(2+) from the suspending medium during electron transport. the species examined included the rat, mouse, rabbit, hamster, guinea pig, cow, chicken, turtle, blowfly, yeast and neurospora crassa. the tissues examined included vertebrate liver, kidney, brain, heart, spleen, thyroid and adrenal cortex, and the flight muscle of the blowfly. the mitochondria from all vertebrate tis ...19715129264
isoprenoid phenol and quinone precursors of ubiguinones and dihydroubiguinones (ubiguinones (h 2 )) in fungi.1. ten moulds and two yeasts were analysed for the presence of 2-polyprenylphenols, 2-polyprenyl(h(2))phenols, 6-methoxy-2-polyprenylphenols, 6-methoxy-2-polyprenyl(h(2))phenols, 6-methoxy-2-polyprenyl-1,4-benzoquinones, 6-methoxy-2-polyprenyl(h(2))-1,4-benzoquinones, 5-demethoxyubiquinones, 5-demethoxyubiquinones(h(2)), ubiquinones and ubiquinones(h(2)). 2. the organisms were found to be of three types: (a) those that contained only ubiquinones (aspergillus fumigatus and penicillium brevi-compa ...19715166547
a cytoplasmic protein from neurospora crassa resembling membrane proteins. 19715166638
multiple phenylalanyl-transfer ribonucleic acid synthetase activities in the cytoplasm of neurospora crassa.several trna's specific for a particular amino acid have been shown to exist in multiple, or isoaccepting, forms. there is considerable interest in establishing whether multiple aminoacyl-trna synthetases also exist. we present evidence that the cytoplasm of neurospora crassa contains three chromatographically separable phenylalanyl-trna synthetases distinct from mitochondrial phenylalanyl-trna synthetase. in addition to differences in chromatographic properties the three enzymes exhibit differe ...19695256412
the nature of photoactivation in neurospora crassa. 19675336978
a mitochondrial dihydroorotate oxidase system in neurospora crassa. 19695345581
the biosynthesis of cytidine diphosphate esters in rat liver and neurospora crassa. 19695366838
neurospora crassa glycogen phosphorylase: characterization and kinetics via a new radiochemical assay for phosphorolysis. 19695391473
purification of neurospora crassa endonuclease-resistant dna-rna hybrid. 19705463194
major constituents of the conidial wall of neurospora crassa. 19705494280
mutants of neurospora crassa, escherichia coli and salmonella typhimurium specifically inhibited by carbon dioxide. 19705500023
the specific interaction of chromate with the dual sulfate permease systems of neurospora crassa. 19715550165
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