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formation of mitochondria in neurospora crassa. a study based on mitochondrial density changes.the choline concentration used in the growth medium influences the density of mitochondria produced by the chol-1 mutant of neurospora. isopycnic centrifugation in sucrose gradients can be used to determine the density of mitochondria, and can resolve into two populations, mitochondria derived from a mixture of cells grown at low (1 microg/ml choline chloride) and high (10 microg/ml choline chloride) choline levels. in an experiment in which cells are shifted from low to high choline growth cond ...196514326126
the influence of precursor pool size on mitochondrial composition in neurospora crassa.the chemical composition of mitochondria obtained from exponentially growing neurospora can be varied by addition of choline or amino acids to the culture medium. the variation affects the phospholipid to protein ratio, and the density of mitochondria as determined by isopycnic centrifugation in sucrose gradients. these variations have been observed in biochemical mutant strains as well as wild type cultures. in a choline-requiring strain, two levels of choline supplementation to the medium have ...196514326125
an endonuclease from neurospora crassa specific for polynucleotides lacking an ordered structure. i. purification and properties of the enzyme. 196514284738
a model predicting characteristics of genetic maps in neurospora crassa. 196514287408
mutagenicity of a monofunctional alkylating agent derivative of acridine in neurospora.purple adenine (ad-3) mutants induced in neurospora crassa by 2-methoxy-6-chloro-9-(3-[ethyl-2-chloroethyl] aminopropylamino) acridine dihydrochloride (icr-170) result from intragenic alterations. they are further characterized by low frequencies of leakiness, allelic complementation, and nonpolarized complementation patterns. these characteristics distinguish them from ad-3 mutants induced by x-rays, nitrous acid, and 2-aminopurine.196514242025
induction kinetics and genetic analysis of x-ray-induced mutations in the ad-3 region of neurospora crassa. 196514294078
genetical and biochemical studies of histidine-requiring mutants of neurospora crassa. iv. linkage relationships of hist-3 mutants. 196514292155
genetical and biochemical studies of histidine-requiring mutants of neurospora crassa. 3. correspondence between biochemical characteristics and complementation map position of hist-3 mutants. 196514292154
polysaccharide components of neurospora crassa hyphal walls. 196514290954
cysteine biosynthesis in neurospora crassa. i. the metabolism of sulfite, sulfide, and cysteinesulfinic acid. 196514275135
preferential inhibition of synthesis and methylation of ribosomal rna in neurospora crassa by actidione. 196514265742
[glycine accumulation and protein biosynthesis in choline and riboflavine deficiency in neurospora crassa]. 196514315562
[plate method for the determination of choline with a strain of neurospora crassa]. 19655864838
genetic control of amino acid permeability in neurospora crassa.lester, gabriel (reed college, portland, ore.). genetic control of amino acid permeability in neurospora crassa. j. bacteriol. 91:677-684. 1966.-strains of neurospora crassa resistant to 4-methyltryptophan (4-mt) were isolated from populations of conidia exposed to ultraviolet light. in genetic crosses, 4-mt resistance behaved as a single-gene difference. resistance to 4-mt could not be attributed to a relaxation of control of the formation or the activity of the enzymes of tryptophan biosynthes ...19665883112
growth responses of neurospora crassa to increased partial pressures of the noble gases and nitrogen.buchheit, r. g. (union carbide corp., tonawanda, n.y.), h. r. schreiner, and g. f. doebbler. growth responses of neurospora crassa to increased partial pressures of the noble gases and nitrogen. j. bacteriol. 91:622-627. 1966.-growth rate of the fungus neurospora crassa depends in part on the nature of metabolically "inert gas" present in its environment. at high partial pressures, the noble gas elements (helium, neon, argon, krypton, and xenon) inhibit growth in the order: xe > kr> ar > ne > he ...19665883104
protoperithecia in neurospora crassa: technique for studying their development.a technique is described which facilitates analysis of the development of individual protoperithecia in neurospora crassa. the formation of this organelle proceeds in several clearly discernible steps beginning with the looping of a single hyphal filament and ending with a heavily pigmented, densely packed structure that is the mature protoperithecial structure. the effects of a number of environmental conditions on the development of protoperithecia in two wild types and in a female-sterile mut ...19664410944
conidiation of neurospora crassa. 196621090502
tryptophan transport in neurospora crassa. i. specificity and kinetics.wiley, w. r. (battelle memorial institute, richland, wash.), and w. h. matchett. tryptophan transport in neurospora crassa. i. specificity and kinetics. j. bacteriol. 92:1698-1705. 1966.-the transport of tryptophan in neurospora crassa is mediated by a distinct stereospecific system which is chemically specific for a family of neutral amino acids. the process shows typical saturation kinetics and a sharp decrease in the rate of tryptophan uptake at low temperatures. the q(10) for the process is ...19665958105
purification and properties of ribonuclease n1, an extracellular ribonuclease of neurospora crassa. 19665972365
circadian nature of a rhythm expressed by an invertaseless strain of neurospora crassa.a new strain of neurospora crassa which exhibits a rhythm of conidiation when growing along an agar surface in a growth tube is described. the rhythm has been shown to be circadian for it meets the following criteria: a) the period under constant environmental conditions in the dark is about 24 hours (22.7 hours at 25 degrees ); b) the period is relatively temperature-independent (q(10) is between 0.95 and 1.21 for temperature range of 18 to 35 degrees ); c) the rhythm persists in continuous dar ...19665978549
carbon dioxide stimulation of the ad-3 mutants of neurospora crassa. 19665972694
genetic alteration of pore size and other properties of the neurospora cell wall.trevithick, john r. (university of wisconsin medical school, madison), robert l. metzenberg and donald f. costello. genetic alteration of pore size and other properties of the neurospora cell wall. j. bacteriol. 92:1016-1020. 1966.-several properties of the cell walls of wild type and the osmotic mutant of neurospora crassa have been examined. the peameability of the isolated cell walls to polyethylene glycol and dextran polymers of different molecular weights was investigated by the volume of d ...19665926738
the primary biochemical effect of a morphological mutation in neurospora crassa. 19664381935
regulation of cellulase and cellobiase in neurospora crassa. 19665970513
a study of conidiation in neurospora crassa. 19665971388
[the dependence of p32 incorporation into mycelia of adenine deficient mutant of neurospora crassa on the concentration of adenine in the medium and on the presence of 8-azaadenine]. 19665995918
a cytoplasmic variant of neurospora crassa. 19665968641
genetic controls of recombination i. the recombination-2 gene of neurospora crassa. 19665230008
neurospora crassa cytochrome c. ii. chymotryptic peptides, tryptic peptides, cyanogen bromide peptides, and the complete amino acid sequence. 19665950080
neurospora crassa cytochrome c. i. purification, physical properties, amino acid composition, and peptide maps from "wild type" and "poky" strains. 19665912112
immunoelectrophoretic analysis of cytoplasmic proteins of neurospora crassa. 19665965359
internuclear effects on prototroph frequencies of some crosses in neurospora crassa. 19665961489
an endonuclease from mitochondria of neurospora crassa. 19664287861
the dihydroxy acid dehydratase of neurospora crassa. 19665946629
on the in vitro development of pseudo-tryptophan synthase activity by extracts of neurospora crassa mutant strains td6 and td120. 19665961662
hybridization in vitro between wild-type and mutant forms of glutamate dehydrogenase from neurospora crassa. 19665947152
immunochemical and enzymatic studies and glutamate dehydrogenase and a related mutant protein from neurospora crassa.roberts, d. b. (university of cambridge, cambridge, england). immunochemical and enzymatic studies on glutamate dehydrogenase and a related mutant protein from neurospora crassa. j. bacteriol. 91:1888-1895. 1966.-when an investigation was made of the inhibition of neurospora glutamate dehydrogenase by bivalent and univalent antibodies, it was shown that the enzyme inhibition is not complete even with excess antibodies. the residual activity was some three times greater with bivalent antibodies, ...19665937244
interrelationships of sulfur amino acids in the pool and cellular protein of neurospora crassa. 19665961305
a repressible alkaline phosphatase in neurospora crassa. 19665946607
on the nature of folate derivatives of neurospora crassa and the effect of sulphanilamide. 19665989524
the dna content of centrioles of neurospora crassa during divisions i and iv of ascosporogenesis. 19665959217
a new incompatibility locus in neurospora crassa. 19665919333
the utilization of leaky ad-3 mutants of neurospora crassa in heterokaryon tests for allelic complementation. 19665918504
amino acid production by amitochondrial fraction of neurospora crassa. 19665220957
inositol deficiency resulting in death: an explanation of its occurrence in neurospora crassa.the incorporation of radio-active inositol and choline into the cytoplasmzic membranes of inositol-and choline-auxotrophic mnutants of neuros-pora crassa revealed that the membrane of particles which contain proteases is relatively poor in lecithin and rich in inositol-phospholipid. in mycelia of the mutant requiring inositol, grown in a suboptimum amount of exogenous inositol, the strlctural initegrity of the protease particles is lost, and the bulk of intracellular protease activity is recover ...19665908969
evidence of a microsomal factor required for in vitro development of pseudo-triptophan synthase activity by extracts of neurospora crassa. 19665941363
cobalt toxicity and iron metabolism in neurospora crassa.1. increasing concentrations of cobalt in the medium result in increased production of an iron-binding compound and a corresponding fall in catalase activity of neurospora crassa. 2. cobalt rapidly depletes the medium of iron by enhancing the rate of iron uptake by the mycelium. 3. with toxic amounts of cobalt there is a fall in bound (59)fe and haem (59)fe as well as a decreased incorporation of [2-(14)c]glycine into the mycelial haem fraction. the production of the iron-binding compound preced ...19665938658
amino acid transport in neurospora crassa. bnwl-280. 19665301139
regulation of enzyme synthesis in neurospora crassa. bnwl-480. 19665301008
susceptibility and resistance of several fungi to microbial lysis.potgieter, h. j. (cornell university, ithaca, n.y.), and m. alexander. susceptibility and resistance of several fungi to microbial lysis. j. bacteriol. 91:1526-1532. 1966.-strains of streptomyces, nocardia, and pseudomonas capable of lysing hyphae of fusarium solani or neurospora crassa were obtained by selective culture, but attempts to isolate an organism lysing rhizoctonia solani failed. when provided with f. solani or n. crassa as carbon sources, the actinomycetes tested produced beta-(1 --> ...19665929777
nonessentiality of boron in fungi and the nature of its toxicity.an investigation was undertaken to determine whether any of the following fungi had a requirement for boron (b): saccharomyces cerevisiae, aspergillus niger, neurospora crassa, and penicillium chrysogenum. boron was unessential, and hence a study was made of the concentrations of b that reduced the growth of s. cerevisiae and p. chrysogenum and the mode of action of the b toxicity. fifty and 4000 mg b/liter, respectively, significantly (5% level) reduced the growth of the latter 2 species.in bot ...196616656256
acyl derivatives of homoserine as substrates for homocysteine synthesis in neurospora crassa, yeast, and escherichia coli. 196712325384
regulation of glutamine synthetase. ii. patterns of feedback inhibition in microorganisms.the feedback inhibition of glutamine synthetase was investigated by use of partially purified enzyme preparations from salmonella typhimurium, micrococcus sodonensis, pseudomonas fluorescens, bacillus cereus, bacillus licheniformis, clostridium pasteurianum, rhodospirillum rubrum, neurospora crassa, candida utilis, and chlorella pyrenoidosa. inhibition analyses indicated that the enzyme of each organism can be effectively regulated with mixtures of end products from the diverse pathways of gluta ...19676025412
thiamine-dependent accumulation of tetramethylpyrazine accompanying a mutation in the isoleucine-valine pathway.a mutant of corynebacterium glutamicum was found to accumulate high concentrations of a material which crystallized upon cooling of the broth. the compound was identified as tetramethylpyrazine. the mutant was found to require isoleucine, valine, leucine, and pantothenate for growth. all four requirements probably result from the loss of a single enzyme of the isoleucine-valine pathway. since similar mutants of neurospora crassa accumulate acetoin, the present mutant probably forms tetramethylpy ...19676039355
a repressible acid phosphatase in neurospora crassa. 19676040372
localization of structural polymers in the cell wall of neurospora crassa.the distribution and localization of structural polymers in the cell wall of neurospora crassa has been studied by selective removal and light and electron microscope examination. observations with the light microscope indicated that each polymer by itself can provide structural integrity to the cell wall. examination by electron microscopy showed that the cell wall consists of an outer layer of thick fibrils, identified chemically as a glucan-peptide-galactosamine complex, and an inner layer ma ...19676055990
the nature of photoactivation in neurospora crassa. 19675336978
ribosomes and ribosomal protein from neurospora crassa. i. physical, chemical, and immunochemical properties.ribosomes from neurospora crassa, initially characterized by ultracentrifugal and immunochemical analyses, have been used to prepare ribosomal protein for physical, chemical, and immunochemical study. the acrylamide gel disc electrophoretic profiles of neurospora ribosomal protein exhibit a degree of heterogeneity comparable to what has been observed in other systems. only by chemical modification or by aggregation of the protein do alterations in the profile become apparent. disulfide-bond form ...19674962303
ribosomes and ribosomal proteins from neurospora crassa. ii. ribosomal proteins in different wild-type strains and during various stages of development. 19674965202
genetic control of allelic recombination at the histidine-3 locus of neurospora crassa. 19676082625
synchronous mitochondrial division in neurospora crassa.samples of mycelium of neurospora crassa of known age were harvested from agar plates and examined with the electron microscope. the relative volume of the mitochondria was determined for mycelium of different ages. the volume measurements indicated that the mitochondria were dividing synchronously in fronts 6, 13, and 22(1/2) hr behind the growing hyphal tips. the sequence of mitochondrial division is hypothesized to include mitochondrial cupping followed by division which results in closely as ...19676064362
isolation of mutant strains of neurospora crassa from differentially surviving ascospores. 19675588793
a new white ascospore mutant of neurospora crassa. 19674968445
[on phosphorous compounds in preparations of cell walls from neurospora crassa mycelia]. 19675597389
the effects of light on a circadian rhythm of conidiation in neurospora.the expression of a circadian rhythm of conidiation by timex, a strain of neurospora crassa, is inhibited by growth in continuous white light. the action spectrum for this effect has a strong peak (with minor subpeaks) in the blue region of the visible spectrum, and a broad shoulder in the near ultraviolet. this action spectrum suggests that a carotenoid or flavin compound may be the photoreceptor, but does not allow one to determine conclusively whether the receptor is indeed a carotenoid, flav ...196716656687
photoreactivating enzyme from neurospora crassa. 19676077698
altered aspartate transcarbamylase of neurospora crassa responsible for a suppressor effect. 19676066249
ultrastructural studies of microconidium formation in neurospora crassa.microconidiating cultures of "peach-fluffy" (pe, fl; y8743m, l; fgsc #569) were fixed at various times after the initiation of growth and examined with an electron microscope. hyphae from which microconidia form are markedly vacuolated and show a much more extensive system of rough endoplasmic reticulum than young vegetative hyphae. a bulge in the hypha presages the start of microconidium formation, followed by the rupture of the outermost wall layers. a thick collar forms around the protruding ...19676066052
multiple alterations in metabolite uptake in a mutant of neurospora crassa.a temperature-conditional lethal mutant of neurospora crassa, un-t (55701), was resistant to neutral amino acid analogues by virtue of a decreased ability to transport these analogues and their natural congeners across the cell membrane. the uptake of acidic, but not basic, amino acids was also impaired, as was the uptake of potassium ions. after preincubation above the tolerated temperature, the ability to take up a still wider variety of metabolites was greatly reduced. protoplasts of the muta ...19676057809
catabolism of l-arginine in neurospora crassa. 19675583768
a survey of new morphological mutants in neurospora crassa. 19675583730
the occurrence of two dehydroquinases in neurospora crassa, one constitutive and one inducible. 19674966264
linkage relations of new morphological mutants in linkage group v of neurospora crassa. 19674868675
the serine biosynthetic pathway in neurospora crassa. 19676077050
amino acid substitutions in mutant forms of histidinol dehydrogenase from neurospora crassa.amino acid changes in the enzyme l-histidinol dehydrogenase (l-histidinol-nad oxidoreductase, ec 1.1.1.23) have been determined between the wild-type neurospora crassa and two temperature-sensitive mutants. comparison was made between amino acid analyses of peptides of differing electrophoretic and chromatographic mobilities resulting from tryptic and chymotryptic digestion of protein from wild-type and mutant k26, and wild-type and mutant k445 strains, respectively. the analyses demonstrate the ...19676056636
the size distribution of x-ray induced multilocus deletions in the ad-3 region of neurospora crassa. 19675584575
"no-growth"-complementation in forced heterokaryons from sorbose-resistant (transport-defective) neurospora crassa mutants. 19674385815
involvement of a b-type cytochrome in the assimilatory nitrate reductase of neurospora crassa. 19674384346
3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthetase as an "allosteric" function and ligand interactions with this enzyme from neurospora crassa. 19676064585
mitochondrial and cytoplasmic polysomes from neurospora crassa. 19676055444
properties of a phosphatidylmonomethylethanolamine n-methyl-transferase from neurospora crassa. 19676060457
argininosuccinate synthetase of neurospora crassa. 19676078087
actinomycin d-induced phenotypic reversion in neurospora crassa. 19676077527
phosphoglucomutase mutants and morphological changes in neurospora crassa. 19675233848
ribosomal rna and ribosomes from mitochondria of neurospora crassa. 19675233834
regulation of the "malic" enzyme in neurospora crassa. 19674383262
the accumulation of argininosuccinate in neurospora crassa. ii. inhibition of arginyl-trna synthesis by argininosuccinate. 19676053229
the accumulation of argininosuccinate in neurospora crassa. i. elevated ornithine carbamoyl transferase with high concentrations of arginine. 19676053228
ultraviolet-sensitive mutants in neurospora crassa. 19676061668
glutamic acid dehydrogenases in quiescent and germinating conidia of neurospora crassa. 19676038892
[purification of ribonucleases of neurospora crassa and their properties]. 19675626108
the catabolism of l-arginine by neurospora crassa. 19676048315
studies of a homoserineless bradytroph of neurospora crassa: demonstration of an altered aspartate beta-semialdehyde dehydrogenase. 19674383000
genetic control of the structure and activity of an enzyme aggregate in the tryptophan pathway of neurospora crassa. 19676052569
the effect of oxygen tension on growth, conidiation, and alcohol production of neurospora crassa. 19676036884
studies on malate dehydrogenases and aspartate aminotransferases from neurospora crassa. 19676035063
structure and composition of the cell wall of neurospora crassa.the structure and composition of the cell walls of hyphae of neurospora crassa were investigated by electron microscopy, chemical analysis, and x-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. the wall consists of two phases: randomly disposed skeletal microfibrils of chitin only and an amorphous matrix which contains both beta-glucans and protein. the protein contains a high percentage of the amides of aspartic and glutamic ...19676027990
lepid content of conidia of neurospora crassa. 19676060645
the role of iron in heme synthesis in neurospora crassa. 19676051578
loss of photorepair ability in conidia of neurospora crassa. 19676055393
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