Publications

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[preparation of neurospora crassa mitochondria, using a corundum disk-grinder and some of its properties]. 19694310964
preparation of isolated macroconidia of neurospora crassa. 19695371447
ferrochelatase in wild-type and in cytoplasmic mutants of neurospora crassa. 196911947230
electron-dense structures in neurospora crassa. ii. lomasome-like structures. 19695370983
electron-dense structures in neurospora crassa. i. mesosomes. 19695370982
genetic control of flanking marker behaviour in an allelic cross of neurospora crassa. 19695370979
ribonucleic acid-degrading enzymes associated with purified ribosomes from neurospora crassa mycelia. 19694306180
phosphoribosyl-aminoimidazole-succinocarboxamide synthetase from neurospora crassa. i. partial purification and properties. 19694239091
proteins of mitochondrial and cytoplasmic ribosomes from neurospora crassa. 19695777034
localization of some amino acid biosynthetic enzymes in cell fractions from the slime mutant of neurospora crassa. 19695780099
mutants in the arom gene cluster of neurospora crassa specific for biosynthetic dehydroquinase. 19695364964
[reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 2. purification and properties of aryl-alcohol: nadp-oxidoreductase from neurospora crassa]. 19694389864
[reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 1. purification and properties of aryl-aldehyde: nadp-oxidoreductase from neurospora crassa]. 19694389863
induction of nad-specific glutamate dehydrogenase in neurospora crassa by addition of glutamate to the media. 19694307386
cytoplasmic mutants selected from continuously growing cultures of neurospora crassa. 196917248432
high frequency of 3:1 tetrad ratios for the pdx-1 locus of neurospora crassa. 19695797809
non-reciprocal recombination at the pan-2 locus in neurospora crassa. 19695797808
regulation of nad-specific alcohol dehydrogenases in neurospora crassa. 19694305614
genetic determinants of circadian rhythmicity in neurospora.timex, a strain of neurospora crassa which exhibits a circadian rhythm of conidia formation in growth-tube cultures, has been found to differ from wild-type strains by two genes. one gene, inv, is responsible for an invertase deficiency, whereas the second gene, bd, is of unknown function. both genes map independently from other genes known to induce neurospora rhythmicity. the inv gene is not essential for the timex phenotype because bd strains express that phenotype on certain media. although ...19695773032
neurospora mutant exhibiting hyperproduction of amylase and invertase.a mutant strain of neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. the resulting increases of the two extracellular enzymes were prevented by actinomycin d, cycloheximide, and glycerol. the starving cells of the mutant strain produced amylase and invertase de novo, as evidenced by incorporation of radioactive amino acids i ...19695773010
photochemical studies of the carotenoid biosynthetic pathway in neurospora crassa. 19695772972
gene-enzyme relationships in neurospora invertase.a spontaneous, single-gene mutation responsible for a total lack of invertase activity in neurospora crassa is described. the mutation is believed to lie in the structural gene for invertase, since an immunologically cross-reacting protein is made by the mutant strain. in addition, there was no evidence for a defect in regulation of invertase activity or synthesis by the following criteria. (i) the invertaseless condition was recessive in heterokaryons; (ii) no invertase inhibitor was found in m ...19694975154
amino acid transport in neurospora crassa. i. properties of two amino acid transport systems. 19694975665
studies concerning the biochemical genetics and physiology of activity and allosteric inhibition mutants of neurospora crassa 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase. 19695808705
crossing-over across the centromere in the mating-type chromosome of neurospora crassa. 19695800692
a sequence of colonial mutants with practical application for mutation studies in neurospora crassa. 19695399227
amino acid induction of conidiation and morphological alterations in wild type and morphological mutants of neurospora crassa. 19695384626
treatment of three-point linkage data in neurospora crassa. 19695371748
new markers and map sequences in neurospora crassa, with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. 19695367562
interconvertible forms of glycogen synthetase in neurospora crassa. 19695363997
genetic factors affecting allelic recombination at the histidine-3 locus of neurospora crassa. 19695345763
utilization of betaine aldehyde by choline (chol-) mutants of neurospora crassa. 19694915435
regulation of amino acid assimilation in a strain of neurospora crassa lacking basic amino acid transport activity. 19695368248
[comparative study of the alanine-glyoxylate transaminase and malate synthetase in neurospora crassa]. 19695372863
ribosomal changes related to changes in the rate of growth in neurospora crassa mycelia. 19695366938
pyrimidine synthesis in neurospora crassa: regulation of enzyme activities.the regulation of several enzymes involved in pyrimidine biosynthesis in neurospora crassa has been studied. elevation of atcase (l-aspartate carbamoyltransferase) activity is found in all pyrimidine-requiring mutants when they are starved for uridine. dhoase (dihydroorotase) is an unstable enzyme, and it is impossible to conclude what type of regulation, if any, controls this enzyme. dhodehase (dihydroorotate dehydrogenase) activity shows a marked elevation in uridine-starved pyr-2 cultures, a ...19695361219
pyrimidine synthesis in neurospora crassa: gene-enzyme relationships.a new series of pyrimidine-requiring mutants of neurospora has been isolated and all enzymes involved in pyrimidine biosynthesis are represented by at least one mutant. among these mutants is included a single isolate for a new locus, pyr-6. this mutant is deficient in dihydroorotase (dhoase) and represents the only enzymatic step in orotate synthesis for which no mutant previously had been found. this mutant, which mapped genetically on the right arm of linkage group v, is unlinked to any of th ...19695361218
aging and repair in neurospora crassa studied by ultraviolet irradiation. 19695352952
isolation, characterization, and genetic analysis of osmotic mutants of neurospora crassa. 19694922974
role of molybdenum in the regulation of nitrate reductase in neurospora crassa. 19694392507
regulation of nitrate reductase activity in neurospora crassa. 19694314882
inhibition of protein synthesis in neurospora crassa by 8-azaguanine. 19694245416
[immunochemistry and electrophoresis of the conidiation of neurospora crassa]. 19695357827
polyoxin d, a competitive inhibitor of udp-n-acetylglucosamine: chitin n-acetylglucosaminyltransferase in neurospora crassa. 19695353899
particulate enzymes of the glyoxylate cycle in neurospora crassa. 19694390938
purification and properties of the aromatic (arom) synthetic enzyme aggregate of neurospora crassa. 19695354272
in vivo and in vitro complementation between dhq synthetase mutants in the arom gene cluster of neurospora crassa. 19695399249
isolation and characterization of nuclease mutants in neurospora crassa. 19695365296
purification and crystallization of ribonuclease n1 from neurospora crassa. 19695348588
mutants of neurospora crassa permeable to histidinol. 196917248455
cycloheximide resistant amino acid incorporation into mitochondrial protein from neurospora crassa in vivo. 196911947193
specificity of mitochondrial and cytoplasmic ribosomes from neurospora crassa in poly-u dependent cell free systems. 196911947164
the control of allelic recombination at histidine loci in neurospora crassa. 19695799350
the gemini-xi s-4 spaceflight radiation interaction experiment. ii. analysis of survival levels and forward-mutation frequencies in neurospora crassa. 19695797679
purification of a trifunctional enzyme, catalysing three steps of the histidine pathway, from neurospora crassa.1. a procedure is described for the purification of an enzyme from neurospora crassa that has three catalytic functions. these are 1-n-(5'-phosphoribosyl)-atp pyrophosphohydrolase, 1-n-(5'-phosphoribosyl)-amp cyclohydrolase and histidinol dehydrogenase (l-histidinol-nad oxidoreductase, ec 1.1.1.23), and are responsible for the catalysis of reactions 2, 3 and 10 in the histidine pathway. the ratio of these three catalytic activities remains approximately the same throughout the purification proce ...19694309307
urease defective mutants in neurospora crassa. 19694903630
locus-specific changes in cell wall composition characteristic of osmotic mutants of neurospora crassa.the osmotic phenotype of neurospora crassa is characterized by inhibition of growth at high osmolalities of growth medium. mutations at six osmotic loci of linkage group i were examined to assess the biochemical and physiological effects of these mutants. isolated cell walls from 23 osmotic strains were compared with the wild type with regard to quantitative levels of the following components: percentage of total dry weight, total glucose, alkali-soluble glucose, nonglucose carbohydrates, amino ...19695802619
genetic control of phenylalanine and tyrosine biosynthesis in neurospora crassa. 19695384488
the iv-3 mutants of neurospora crassa. ii. activity of acetohydroxy acid synthetase. 19695384481
the iv-3 mutants of neurospora crassa. i. genetic and biochemical characteristics. 19695384480
the isolation of mutants affecting ascus development in neurospora crassa and their analysis by a zygote complementation test. 19695806169
the mitochondrial and cytoplasmic transfer ribonucleic acids of neurospora crassa. 19695799126
variation in "map distance" in neurospora crassa. 19695798519
beta-ketoadipate coa-transferase in neurospora crassa. 19695793125
purification and characterization of a multienzyme complex in the tryptophan pathway of neurospora crassa. 19695769998
the metabolic control of nitrite reductase in neurospora crassa. 19695787239
glycogen phosphorylase of neurospora crassa. 19695787226
alkaline phosphatases of neurospora crassa. i. 19695786781
inhibition by ethionine of the growth of neurospora crassa. 19695786780
catalase of neurospora crassa. 2. electron paramagnetic resonance and chemical properties of the prosthetic group. 1969223625
effects of inorganic pyrophosphate on neurospora crassa phenylalanine transfer ribonucleic acid ligase in heterologous aminoacylation reactions. 19694309130
the amino acid composition of the subunit of the nadp-specific glutamate dehydrogenase from neurospora crassa. 19694390421
studies on the aminoacylation of valine- and alanine-specific transfer rna of escherichia coli by aminoacyl transfer rna synthetases from neurospora crassa and e. coli. 19694883911
effects of tris and dimethylsulfoxide on the aminoacylation of escherichia coli valine transfer rna by neurospora crassa phenylalanyl transfer rna synthetase. 19694890606
puromycin sensitivity of ribosomal label after incorporation of 14c-labelled amino acids into isolated mitochondria from neurospora crassa. 19694899931
regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms.1. aspergillus nidulans, neurospora crassa and escherichia coli were grown on media containing a range of concentrations of nitrate, or ammonia, or urea, or l-glutamate, or l-glutamine as the sole source of nitrogen and the glutamate dehydrogenate and glutamine synthetase of the cells measured. 2. aspergillus, neurospora and escherichia coli cells, grown on l-glutamate or on high concentrations of ammonia or on high concentrations of urea, possessed low glutamate dehydrogenase activity compared ...19694901826
codon recognition by enzymatically mischarged valine transfer ribonucleic acid.direct evidence for the adaptor hypothesis has been obtained by examining the codon recognition of a purified escherichia coli valine transfer ribonucleic acid which was enzymatically mischarged with phenylalanine labeled with carbn-14 by reaction with purified phenylalanyl-transfer ribonucleic acid synthetase from neurospora crassa. the mischarged transfer ribonucleic acid recognized the valine codons but failed to recognize the phenylalanine codon when tested in trinucleotide-directed ribosoma ...19694902680
mitochondrial and cytoplasmic ribosomes from neurospora crassa: characterization of their subunits. 19694903818
multiple phenylalanyl-transfer ribonucleic acid synthetase activities in the cytoplasm of neurospora crassa.several trna's specific for a particular amino acid have been shown to exist in multiple, or isoaccepting, forms. there is considerable interest in establishing whether multiple aminoacyl-trna synthetases also exist. we present evidence that the cytoplasm of neurospora crassa contains three chromatographically separable phenylalanyl-trna synthetases distinct from mitochondrial phenylalanyl-trna synthetase. in addition to differences in chromatographic properties the three enzymes exhibit differe ...19695256412
the facile isolation of a structural phospholipoprotein from hydrogenomon as facilis and neurospora crassa.the paper describes a new and gentle procedure for isolating "structural" phospholipoproteins from organisms and the first such isolation from a procaryotic microbe, hydrogenomonas facilis. the amino acid composition of its protein moiety resembles that of "structural protein" from other sources. although disc gel electrophoresis has shown the protein to be heterogeneous, this is attributed to aggregation.19694978742
organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.ultracentrifugation in sucrose density gradients was employed to estimate the molecular weights and to determine possible physical aggregation of the five enzymes catalyzing steps two to six in the prechorismic acid portion of the polyaromatic synthetic pathway in six species of bacteria: escherichia coli, salmonella typhimurium, aerobacter aerogenes, bacillus subtilis, pseudomonas aeruginosa, and streptomyces coelicolor. the five enzymes were not aggregated in extracts of any of the species exa ...19694979440
mutagen specificity at the ad-3a and inositol loci in neurospora crassa. 19695796512
organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi.centrifugation in sucrose density gradients of partially purified extracts from six species of fungi, i.e., rhizopus stolonifer, phycomyces nitens, absidia glauca (phycomycetes), aspergillus nidulans (ascomycetes), coprinus lagopus, and ustilago maydis (basidiomycetes), indicate that the five enzymes catalyzing steps two to six in the prechorismic acid part of the polyaromatic synthetic pathway sediment together. the sedimentation coefficients for these enzymes are very similar in the six specie ...19695802608
a mitochondrial dihydroorotate oxidase system in neurospora crassa. 19695345581
the biosynthesis of cytidine diphosphate esters in rat liver and neurospora crassa. 19695366838
neurospora crassa glycogen phosphorylase: characterization and kinetics via a new radiochemical assay for phosphorolysis. 19695391473
isolation and characterization of chromatin from neurospora crassa.different preparations of chromatin isolated from mycelia of neurospora crassa were analyzed for dna-associated rna and proteins. the uv absorption spectra, the ultrastructure of chromatin, and the amino acid composition of the acid-extractable proteins were studied. the protein:dna ratios range from 1.5 to 2.8; the rna:dna ratios range from 0.5 to 1.24. uv absorption shows a macimum at 259 mmicro and a minimum at 238-239 mmicro. the e280/e260 ranges from 0.59 to 0.70. electron microscopy reveal ...19694186412
effects of radiation during space flight on microorganisms and plants on the biosatellite ii and gemini xi missions.the results of recent experiments with the lysogenic bacteria, escherichia coli and salmonella typhimurium, the bread mold neurospora crassa and the flowering plant tradescantia on the biosatellite ii and gemini xi missions will be summarized. in the lysogenic bacteria experiment (dr. rudolf h.t. mattoni, nus corporation) on the biosatellite ii mission significant effects of space flight were found on both growth rate and the induction of prophage. in that part of the neurospora experiment on bo ...196911949688
feedback inhibition of glutamine synthetase of neurospora crassa by nicotinamide-adenine dinucleotide. 19684300703
mechanism of choline o-sulphate utilization in fungi.1. the position of the enzyme blocks in a number of parathiotrophic mutants of aspergillus nidulans a 69 and mutants a and c of a biotinless mutant of aspergillus nidulans were examined by nutritional and heterokaryosis experiments and by assay in vitro of enzyme systems and specific enzymes. 2. the mutants were in five groups: a and c blocked at sulphate transport; gamma at atp sulphurylase; iota at adenosine 5'-sulphatophosphate kinase; eta at the adenosine 3'-phosphate 5'-sulphatophosphate re ...19685637353
induced mutation in uv-sensitive mutants of aspergillus nidulans and neurospora crassa. 19685672966
an acetohydroxy acid synthetase from neurospora crassa. 19685677177
the metabolism of aromatic acids by micro-organisms. metabolic pathways in the fungi.1. the metabolic pathways of aromatic-ring fission were examined in a range of fungal genera that utilize several compounds related to lignin. 2. most of the genera, after growth on p-hydroxybenzoate, protocatechuate or compounds that are degraded to the latter (e.g. caffeate, ferulate or vanillate), rapidly oxidized these compounds, but not catechol. 3. such genera possessed a protocatechuate 3,4-oxygenase and accumulated beta-carboxymuconate as the product of protocatechuate oxidation. this en ...19685691754
the metabolism of aromatic acids by micro-organisms. a reassessment of the role of o-benzoquinone as a product of protocatechuate metabolism by fungi.1. the addition of aniline to cultures of several yeasts, fusarium oxysporum and neurospora crassa growing with protocatechuate as sole carbon source resulted in the precipitation of dianilino-o-benzoquinone (anil). this product was also formed, however, if the medium was uninoculated. 2. the physical presence of yeast cells (living or dead) increased the anil yields in debaryomyces subglobosus cultures. 3. no anil was formed if p-hydroxybenzoate was the growth substrate. 4. o-benzoquinone was a ...19685691755
thymidine kinase: evidence for its absence from neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid. 19685729618
[proteins from neurospora crassa. 1. immunochemical analysis of the cytoplasmic proteins from neurospora crassa and various mutants]. 19684973222
[proteins from neurospora crassa. 3. agar gel and immunoelectrophoretic analysis of enzymes and isoenzymes in various neurospora crassa strains]. 19684973224
comparative regulation of isoenzymic 3-deoxy-d-arabino-heptulosonate 7-phosphate synthetases in microorganisms.the independent control of regulatory isoenzymes by different metabolites constitutes one well-known pattern of control in branched metabolic pathways. this pattern was previously found to be widely distributed in the aromatic amino acid pathway of microorganisms in the case of the first enzyme of the sequence, 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthetase. the comparative stability of the isoenzymes as well as the effect of aromatic amino acids in the growth medium upon the leve ...19684867215
the pathways of thiamine biosynthesis. some factors controlling synthesis of the pyrimidine moiety by thiamine auxotrophs of neurospora crassa. 19684875319
a repressible alkaline phosphatase in neurospora crassa. ii. isolation and chemical properties. 19685653190
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