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in vitro formation of an active multienzyme complex in the tryptophan pathway of neurospora crassa.a multienzyme complex that catalyzes the anthranilate synthetase, phosphoribosylanthranilate (pra) isomerase, and indoleglycerolphosphate (ingp) synthetase reactions was produced in vitro when extracts from a tryp-1 mutant and a tryp-2 mutant of neurospora crassa were mixed. the sedimentation values and the molecular weights for the interacting components obtained from the mutants were estimated by sucrose gradients and by gel filtration on sephadex columns. the component coded for by the tryp-2 ...19695264137
enzyme activities during the asexual cycle of neurospora crassa. 3. nicotinamide adenosine diphosphate glycohydrolase. 19694391099
glutamine synthetase of neurospora crassa. inactivation by urea and protection by some substrates and allosteric effectors. 19694311182
some characteristics of the d-glucose-6-phosphate: cycloaldolase (nad+ dependent) from neurospora crassa. 19694310378
some aspects of complementation with carotenogrenic al loci in neurospora crassa. 19695357122
repressible alkaline phosphatase in neurospora crassa. 3. enzymatic properties. 19694981068
incorporation in vivo of 14c-labelled amino acids into the proteins of mitochondrial ribosomes from neurospora crassa sensitive to cycloheximide and insensitive to chloramphenicol. 19695356628
mutants affecting thymidine metabolism in neurospora crassa.when (14)c-thymidine labeled only in the ring is administered to neurospora crassa, the majority of the recovered label is found in the ribonucleic acid (rna). three mutants were isolated in which different steps are blocked in the pathway that converts the pyrimidine ring of thymidine to an rna precursor. evidence from genetic, nutritional, and accumulation studies with the three mutants shows the pathway to proceed as follows: thymidine --> thymine --> 5-hydroxymethyluracil --> 5-formyluracil ...19695344102
[cytochrome oxidase and associated with it a labelled protein from 14c-labelled mitochondria of neurospora crassa]. 19694310965
[preparation of neurospora crassa mitochondria, using a corundum disk-grinder and some of its properties]. 19694310964
preparation of isolated macroconidia of neurospora crassa. 19695371447
ferrochelatase in wild-type and in cytoplasmic mutants of neurospora crassa. 196911947230
electron-dense structures in neurospora crassa. ii. lomasome-like structures. 19695370983
electron-dense structures in neurospora crassa. i. mesosomes. 19695370982
genetic control of flanking marker behaviour in an allelic cross of neurospora crassa. 19695370979
isolation and characterization of nuclease mutants in neurospora crassa. 19695365296
purification and crystallization of ribonuclease n1 from neurospora crassa. 19695348588
genetic control of phenylalanine and tyrosine biosynthesis in neurospora crassa. 19695384488
non-reciprocal recombination at the pan-2 locus in neurospora crassa. 19695797808
regulation of nad-specific alcohol dehydrogenases in neurospora crassa. 19694305614
genetic determinants of circadian rhythmicity in neurospora.timex, a strain of neurospora crassa which exhibits a circadian rhythm of conidia formation in growth-tube cultures, has been found to differ from wild-type strains by two genes. one gene, inv, is responsible for an invertase deficiency, whereas the second gene, bd, is of unknown function. both genes map independently from other genes known to induce neurospora rhythmicity. the inv gene is not essential for the timex phenotype because bd strains express that phenotype on certain media. although ...19695773032
the iv-3 mutants of neurospora crassa. ii. activity of acetohydroxy acid synthetase. 19695384481
the iv-3 mutants of neurospora crassa. i. genetic and biochemical characteristics. 19695384480
the isolation of mutants affecting ascus development in neurospora crassa and their analysis by a zygote complementation test. 19695806169
the mitochondrial and cytoplasmic transfer ribonucleic acids of neurospora crassa. 19695799126
variation in "map distance" in neurospora crassa. 19695798519
beta-ketoadipate coa-transferase in neurospora crassa. 19695793125
purification and characterization of a multienzyme complex in the tryptophan pathway of neurospora crassa. 19695769998
the metabolic control of nitrite reductase in neurospora crassa. 19695787239
glycogen phosphorylase of neurospora crassa. 19695787226
alkaline phosphatases of neurospora crassa. i. 19695786781
inhibition by ethionine of the growth of neurospora crassa. 19695786780
ribonucleic acid-degrading enzymes associated with purified ribosomes from neurospora crassa mycelia. 19694306180
phosphoribosyl-aminoimidazole-succinocarboxamide synthetase from neurospora crassa. i. partial purification and properties. 19694239091
proteins of mitochondrial and cytoplasmic ribosomes from neurospora crassa. 19695777034
localization of some amino acid biosynthetic enzymes in cell fractions from the slime mutant of neurospora crassa. 19695780099
mutants in the arom gene cluster of neurospora crassa specific for biosynthetic dehydroquinase. 19695364964
[reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 2. purification and properties of aryl-alcohol: nadp-oxidoreductase from neurospora crassa]. 19694389864
[reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 1. purification and properties of aryl-aldehyde: nadp-oxidoreductase from neurospora crassa]. 19694389863
induction of nad-specific glutamate dehydrogenase in neurospora crassa by addition of glutamate to the media. 19694307386
cytoplasmic mutants selected from continuously growing cultures of neurospora crassa. 196917248432
high frequency of 3:1 tetrad ratios for the pdx-1 locus of neurospora crassa. 19695797809
neurospora mutant exhibiting hyperproduction of amylase and invertase.a mutant strain of neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. the resulting increases of the two extracellular enzymes were prevented by actinomycin d, cycloheximide, and glycerol. the starving cells of the mutant strain produced amylase and invertase de novo, as evidenced by incorporation of radioactive amino acids i ...19695773010
pyrimidine synthesis in neurospora crassa: gene-enzyme relationships.a new series of pyrimidine-requiring mutants of neurospora has been isolated and all enzymes involved in pyrimidine biosynthesis are represented by at least one mutant. among these mutants is included a single isolate for a new locus, pyr-6. this mutant is deficient in dihydroorotase (dhoase) and represents the only enzymatic step in orotate synthesis for which no mutant previously had been found. this mutant, which mapped genetically on the right arm of linkage group v, is unlinked to any of th ...19695361218
aging and repair in neurospora crassa studied by ultraviolet irradiation. 19695352952
isolation, characterization, and genetic analysis of osmotic mutants of neurospora crassa. 19694922974
role of molybdenum in the regulation of nitrate reductase in neurospora crassa. 19694392507
regulation of nitrate reductase activity in neurospora crassa. 19694314882
inhibition of protein synthesis in neurospora crassa by 8-azaguanine. 19694245416
[immunochemistry and electrophoresis of the conidiation of neurospora crassa]. 19695357827
polyoxin d, a competitive inhibitor of udp-n-acetylglucosamine: chitin n-acetylglucosaminyltransferase in neurospora crassa. 19695353899
particulate enzymes of the glyoxylate cycle in neurospora crassa. 19694390938
purification and properties of the aromatic (arom) synthetic enzyme aggregate of neurospora crassa. 19695354272
regulation of amino acid assimilation in a strain of neurospora crassa lacking basic amino acid transport activity. 19695368248
[comparative study of the alanine-glyoxylate transaminase and malate synthetase in neurospora crassa]. 19695372863
ribosomal changes related to changes in the rate of growth in neurospora crassa mycelia. 19695366938
pyrimidine synthesis in neurospora crassa: regulation of enzyme activities.the regulation of several enzymes involved in pyrimidine biosynthesis in neurospora crassa has been studied. elevation of atcase (l-aspartate carbamoyltransferase) activity is found in all pyrimidine-requiring mutants when they are starved for uridine. dhoase (dihydroorotase) is an unstable enzyme, and it is impossible to conclude what type of regulation, if any, controls this enzyme. dhodehase (dihydroorotate dehydrogenase) activity shows a marked elevation in uridine-starved pyr-2 cultures, a ...19695361219
effects of radiation during space flight on microorganisms and plants on the biosatellite ii and gemini xi missions.the results of recent experiments with the lysogenic bacteria, escherichia coli and salmonella typhimurium, the bread mold neurospora crassa and the flowering plant tradescantia on the biosatellite ii and gemini xi missions will be summarized. in the lysogenic bacteria experiment (dr. rudolf h.t. mattoni, nus corporation) on the biosatellite ii mission significant effects of space flight were found on both growth rate and the induction of prophage. in that part of the neurospora experiment on bo ...196911949688
catalase of neurospora crassa. 2. electron paramagnetic resonance and chemical properties of the prosthetic group. 1969223625
organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.ultracentrifugation in sucrose density gradients was employed to estimate the molecular weights and to determine possible physical aggregation of the five enzymes catalyzing steps two to six in the prechorismic acid portion of the polyaromatic synthetic pathway in six species of bacteria: escherichia coli, salmonella typhimurium, aerobacter aerogenes, bacillus subtilis, pseudomonas aeruginosa, and streptomyces coelicolor. the five enzymes were not aggregated in extracts of any of the species exa ...19694979440
effects of inorganic pyrophosphate on neurospora crassa phenylalanine transfer ribonucleic acid ligase in heterologous aminoacylation reactions. 19694309130
isolation and characterization of chromatin from neurospora crassa.different preparations of chromatin isolated from mycelia of neurospora crassa were analyzed for dna-associated rna and proteins. the uv absorption spectra, the ultrastructure of chromatin, and the amino acid composition of the acid-extractable proteins were studied. the protein:dna ratios range from 1.5 to 2.8; the rna:dna ratios range from 0.5 to 1.24. uv absorption shows a macimum at 259 mmicro and a minimum at 238-239 mmicro. the e280/e260 ranges from 0.59 to 0.70. electron microscopy reveal ...19694186412
the facile isolation of a structural phospholipoprotein from hydrogenomon as facilis and neurospora crassa.the paper describes a new and gentle procedure for isolating "structural" phospholipoproteins from organisms and the first such isolation from a procaryotic microbe, hydrogenomonas facilis. the amino acid composition of its protein moiety resembles that of "structural protein" from other sources. although disc gel electrophoresis has shown the protein to be heterogeneous, this is attributed to aggregation.19694978742
the amino acid composition of the subunit of the nadp-specific glutamate dehydrogenase from neurospora crassa. 19694390421
multiple phenylalanyl-transfer ribonucleic acid synthetase activities in the cytoplasm of neurospora crassa.several trna's specific for a particular amino acid have been shown to exist in multiple, or isoaccepting, forms. there is considerable interest in establishing whether multiple aminoacyl-trna synthetases also exist. we present evidence that the cytoplasm of neurospora crassa contains three chromatographically separable phenylalanyl-trna synthetases distinct from mitochondrial phenylalanyl-trna synthetase. in addition to differences in chromatographic properties the three enzymes exhibit differe ...19695256412
a mitochondrial dihydroorotate oxidase system in neurospora crassa. 19695345581
neurospora crassa glycogen phosphorylase: characterization and kinetics via a new radiochemical assay for phosphorolysis. 19695391473
studies on the aminoacylation of valine- and alanine-specific transfer rna of escherichia coli by aminoacyl transfer rna synthetases from neurospora crassa and e. coli. 19694883911
effects of tris and dimethylsulfoxide on the aminoacylation of escherichia coli valine transfer rna by neurospora crassa phenylalanyl transfer rna synthetase. 19694890606
puromycin sensitivity of ribosomal label after incorporation of 14c-labelled amino acids into isolated mitochondria from neurospora crassa. 19694899931
regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms.1. aspergillus nidulans, neurospora crassa and escherichia coli were grown on media containing a range of concentrations of nitrate, or ammonia, or urea, or l-glutamate, or l-glutamine as the sole source of nitrogen and the glutamate dehydrogenate and glutamine synthetase of the cells measured. 2. aspergillus, neurospora and escherichia coli cells, grown on l-glutamate or on high concentrations of ammonia or on high concentrations of urea, possessed low glutamate dehydrogenase activity compared ...19694901826
codon recognition by enzymatically mischarged valine transfer ribonucleic acid.direct evidence for the adaptor hypothesis has been obtained by examining the codon recognition of a purified escherichia coli valine transfer ribonucleic acid which was enzymatically mischarged with phenylalanine labeled with carbn-14 by reaction with purified phenylalanyl-transfer ribonucleic acid synthetase from neurospora crassa. the mischarged transfer ribonucleic acid recognized the valine codons but failed to recognize the phenylalanine codon when tested in trinucleotide-directed ribosoma ...19694902680
mitochondrial and cytoplasmic ribosomes from neurospora crassa: characterization of their subunits. 19694903818
mutagen specificity at the ad-3a and inositol loci in neurospora crassa. 19695796512
organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi.centrifugation in sucrose density gradients of partially purified extracts from six species of fungi, i.e., rhizopus stolonifer, phycomyces nitens, absidia glauca (phycomycetes), aspergillus nidulans (ascomycetes), coprinus lagopus, and ustilago maydis (basidiomycetes), indicate that the five enzymes catalyzing steps two to six in the prechorismic acid part of the polyaromatic synthetic pathway sediment together. the sedimentation coefficients for these enzymes are very similar in the six specie ...19695802608
the biosynthesis of cytidine diphosphate esters in rat liver and neurospora crassa. 19695366838
photochemical studies of the carotenoid biosynthetic pathway in neurospora crassa. 19695772972
gene-enzyme relationships in neurospora invertase.a spontaneous, single-gene mutation responsible for a total lack of invertase activity in neurospora crassa is described. the mutation is believed to lie in the structural gene for invertase, since an immunologically cross-reacting protein is made by the mutant strain. in addition, there was no evidence for a defect in regulation of invertase activity or synthesis by the following criteria. (i) the invertaseless condition was recessive in heterokaryons; (ii) no invertase inhibitor was found in m ...19694975154
amino acid transport in neurospora crassa. i. properties of two amino acid transport systems. 19694975665
studies concerning the biochemical genetics and physiology of activity and allosteric inhibition mutants of neurospora crassa 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase. 19695808705
crossing-over across the centromere in the mating-type chromosome of neurospora crassa. 19695800692
a sequence of colonial mutants with practical application for mutation studies in neurospora crassa. 19695399227
amino acid induction of conidiation and morphological alterations in wild type and morphological mutants of neurospora crassa. 19695384626
treatment of three-point linkage data in neurospora crassa. 19695371748
new markers and map sequences in neurospora crassa, with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. 19695367562
interconvertible forms of glycogen synthetase in neurospora crassa. 19695363997
genetic factors affecting allelic recombination at the histidine-3 locus of neurospora crassa. 19695345763
utilization of betaine aldehyde by choline (chol-) mutants of neurospora crassa. 19694915435
non-nuclear inheritance of uv sensitivity in neurospora crassa. 19685759970
the effect of cycloheximide on carotenoid biosynthesis in neurospora crassa. 19685704312
anthranilic acid as a feedback inhibitor of glutamine synthetase of neurospora crassa. 19685704311
the in vivo iron donor for haem synthesis in neurospora crassa. 19684239950
the formation of 5-formyluracil by cell-free preparations from neurospora crassa. 19684387266
induced macroconidia formation in neurospora crassa. 19685721155
[inclusion of adenine-8-c14 into the ribosomes and polyribosomes of germinating conidia and mycelium of a neurospora crassa 28610 ad. strain deficient in adenine]. 19685747531
in vivo regulation of intermediate reactions in the pathway of tryptophan biosynthesis in neurospora crassa.the in vivo regulation of intermediate reactions in the pathway of tryptophan synthesis in neurospora crassa was examined in a double mutant (tr-2, tr-3) which lacks the functions of the first and last enzymes in the pathway from chorismic acid to tryptophan. the double mutant can convert anthranilic acid to indole and indole-3-glycerol, and the production of these indolyl compounds by germinated conidia was used to estimate the activity of the intermediate enzymes in the pathway. indole-synthes ...19685726311
the exogenous environment as a factor in the control of the 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase of neurospora crassa. 19685729957
a new locus in the tryptophan pathway of neurospora crassa. 19685708292
specific inhibition by atp and other properites of an endonuclease of neurospora crassa. 19685701229
electrophoretic pattern of and amino acid incorporation in vitro into the insoluble mitochondrial protein of neurospora crassa wild type and mi-1 mutant. 196811945305
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