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cooperation of mitochondrial and nuclear genes specifying the mitochondrial genetic apparatus in neurospora crassa.enzymes involved in the expression of the mitochondrial genome in neurospora crassa are induced by chloramphenicol and ethidium bromide, which block transcription and translation of mitochondrial dna. it is concluded that most, if not all, proteins of the mitochondrial genetic apparatus are coded by nuclear genes, synthesized on cytoplasmic ribosomes, and controlled by a repressor-like mitochondrial gene product. a model explaining the coordination of nuclear and mitochondrial division cycles by ...19724260903
genetic characterization of diethylnitrosamine-induced purple adenine (ad-3) mutants in neurospora crassa. 19724260579
delta-aminolaevulinate dehydratase, the regulatory enzyme of the haem-biosynthetic pathway in neurospora crassa.the activity of delta-aminolaevulinate dehydratase is very low in the mould neurospora crassa compared with the activities detected in bacterial and animal systems. the enzyme is inducible in iron-deficient cultures by addition of iron and is repressed by protoporphyrin. the properties of the purified enzyme indicate its allosteric nature and susceptibility to feedback inhibition by coproporphyrinogen iii. neurospora extracts also contain a protein inhibitor of the enzyme and a small-molecule ac ...19724265023
isolation and classification of extranuclear mutants of neurospora crassa.four extranuclear mutants, [exn-1], [exn-2],[exn-4], and [stp-c], were obtained from n-methyl-n'-nitro-n-nitrosoguanidine-treated conidia and mycelium of neurospora crassa. the three exn mutants grow with a pronounced lag from conidia and ascospores and are female fertile, whereas [stp-c] has a stop-start growth phenotype and is female sterile. the mitochondria from all four mutants are deficient in cytochromes a+a(3) and b, but contain an excess of cytochrome c. on the basis of growth and ferti ...19724262391
cell wall alterations associated with the hyperproduction of extracellular enzymes in neurospora crassa.a pleiotropic mutation in neurospora (exo-1), which confers derepression of alpha-amylase, glucoamylase, beta-fructofuranosidase, and trehalase, appears to also affect the composition of the cell wall. segregants resulting from the backcross of exo-1 to the wild-type strain from which it derived are altered in the ratio of galactosamine to glucosamine in hydrolysates of isolated cell walls. conidial cell walls exhibit a marked decrease in the amount of galactosamine in both exo-1 and exo-1(+) st ...19724262302
pyruvate kinase of neurospora crassa: effect of various ligands on the rate of inactivation by protein denaturants. 19724262270
conidial repression by phenethyl alcohol and its serological consequences in neurospora crassa. 19724265955
the effects of branched chain fatty acid incorporation into neurospora crassa membranes. 19724265873
a possible causal relationship between iron deficiency, inhibition of dna synthesis and reduction of meiotic recombination frequency in neurospora crassa. 19724264533
complementation among cytoplasmic mutants of neurospora crassa. 19724262636
analysis of a case of mutagen specificity in neurospora crassa. iv. interactions between uv and three chemical mutagens. 19724262635
development of fertile fruit bodies in a single-strain culture of neurospora crassa. 19724262618
an unlinked mutation affecting control of purine metabolism in a revertant of an ad-7 auxotroph of neurospora crassa lacking the phosphoribosylpyrophosphate amidotransferase. 19724259695
genes conferring resistance to 8-aza adenine in neurospora crassa and the variability of resistant alleles in the aza-1 locus with respect to excretion of purines. 19724259694
novel mutation causing derepression of several enzymes of sulfur metabolism in neurospora crassa.a group of enzymes of sulfur metabolism (arylsulfatase, cholinesulfatase, and a number of others) are normally repressed in neurospora crassa by an abundant supply of a "favored" sulfur source such as methionine or inorganic sulfate. a mutant called scon(c) was isolated in which the formation of each of these enzymes is largely or completely nonrepressible. the structural genes for three of these enzymes have been mapped; scon(c) is not linked to any of them. it is also not linked to cys-3, anot ...19724257980
[interactions between the amino acid pool and l-amino-acid oxidase in neurospora crassa]. 19724268302
genetic control of recombination in neurospora crassa: correlated regulation in unlinked chromosome intervals. 19714258281
genetic and biochemical studies of the hexose monophosphate shunt in neurospora crassa. ii. characterization of biochemical defects of the morphological mutants colonial 2 and colonial 3. 19714397247
genetic and biochemical studies of the hexose monophosphate shunt in neurospora crassa. i. the influence of genetic defects in the pathway on colonial morphology. 19714397246
some details and effects of the premeiotic controls of recombination frequencies in neurospora crassa. 19714258173
long-term cryogenic storage of neurospora crassa spores. 19714257765
[light dependent carotenoid synthesis : vii. cycloheximide or anaerobic conditions as reversible blocks of the sequence of light-induced reactions].anaerobic conditions completely block the synthesis of carotenoids in fusarium aquaeduciuum or neurospora crassa. even after sufficient illumination of the mycelia in the presence of air, the subsequent production of carotenoids in the dark is suppressed entirely when the fungi are transferred to an oxygen-free atmosphere. in turn, restoring aerobic conditions sets off the pigment synthesis in the dark without renewed photoinduction at any time within 48 hours, although the yields of carotenoids ...197124488430
[studies on the conidial differentiation of neurospora crassa. i. chemico-structural organization of conditional conidiation of an amycelial mutant]. 19715129957
enzyme patterns and protein synthesis during synchronous conidiation in neurospora crassa. 19715111767
nh 2 -terminal residues of neurospora crassa proteins.the nh(2)-terminal amino acid composition of the soluble and ribosomal proteins from neurospora crassa mycelia and conidia was determined by the dinitrophenyl method. a nonrandom distribution of nh(2)-terminal amino acids was observed in the complex protein mixtures. glycine, alanine, and serine accounted for 75% of the nh(2)-terminal amino acids, and glycine appeared most frequently in mature proteins of mycelia. the appearance of phenylalanine as one of the major nh(2)-termini in crude conidia ...19715095291
mitochondrial biogenesis in neurospora crassa. i. an ultrastructural and biochemical investigation of the effects of anaerobiosis and chloramphenicol inhibition.the isolation of a new class of mutants permitting facultative anaerobiosis in neurospora crassa is described. backcross analyses to the obligate aerobe prototroph (an(-)) indicate single nuclear gene inheritance (an(-)/an(+)). an(+) and an(-) are indistinguishable in morphology and growth rates under aerobic conditions. anaerobic growth requires nutritional supplements that are dispensable for aerobic growth. conidiogenesis, conidial germination, and vegetative growth rate are suppressed by ana ...19714329155
dihydroorotate-quinone reductase of neurospora crassa mitochondria. 19714259267
magnesium and polyamine levels in neurospora crassa mycelia. 19714256640
biochemical model for the biological methylation of mercury suggested from methylation studies in vivo with neurospora crassa. 19714929974
reversible inactivation and dissociation of glutamine synthetase of neurospora crassa by urea. 19715553349
genetic control of multiple forms of trehalase in neurospora crassa. 19715120655
cryobiology of neurospora crassa. i. freeze response of neurospora crassa conidia. 19715120624
cryobiology of neurospora crassa. ii. alteration in freeze response of neurospora crassa conidia by additives. 19715001009
the site of cytochrome oxidase biosynthesis in neurospora crassa. 19714327452
role of lysine in carnitine biosynthesis in neurospora crassa. 19715090046
regulation of tryptophan biosynthetic enzymes in neurospora crassa.the formation of enzymatic activities involved in the biosynthesis of tryptophan in neurospora crassa was examined under various conditions in several strains. with growth-limiting tryptophan, the formation of four enzymatic activities, anthranilic acid synthetase (aas), anthranilate-5-phosphoribosylpyrophosphate phosphoribosyl transferase (prat), indoleglycerol phosphate synthetase (ingps), and tryptophan synthetase (ts) did not occur coordinately. aas and ts activities began to increase immedi ...19715563869
properties of an extracellular -galactosidase secreted by neurospora crassa.an extracellular beta-galactosidase with an activity optimum at about ph 4 was found to occur in the filtrates of neurospora crassa strain 74a when this mold was grown on certain sugars. this activity accounted for a substantial portion of the beta-galactosidase activity in the culture. the beta-galactosidase in the medium appeared to be a secreted, extracellular enzyme, not a product of cell lysis. the extracellular activity was found to have physical and kinetic properties similar to those of ...19715563865
constitutive mutants in a regulatory gene exerting positive control of quinic acid catabolism in neurospora crassa.in neurospora crassa, evidence has recently been obtained for a cluster of four closely-linked genes controlling the inducible enzymes catalyzing the first three reactions in the catabolism of quinic acid. three of these genes appear to be the structural genes for the three enzymes. the fourth gene, designated qa-1, has been interpreted as having a regulatory function, since qa-1 mutants are pleiotropic types, are noninducible for the three enzymes, and form heterocaryons which complement mutant ...19715283945
a succinic dehydrogenase activity in "mesosomes" of neurospora crassa. 19714250787
absence of a 5s rna complnent in the mitochondrial ribosomes of neurospora crassa. 19715280093
germination and growth of neurospora at low water activities.when the water activity (a(w)) of the medium is lowered by the addition of nacl or nonelectrolytes, an inhibition of germination, growth rate, and total growth is observed in neurospora crassa. inhibition of conidial germination is separable from the other effects and is caused, in large measure, by the loss from conidia in media of low water activity of a substance that is essential for their germination. the substance is detectable in the medium and is also extractable from cultures. it is dia ...197116591899
structural genes for dahp synthase isoenzymes in neurospora crassa. 19715569206
effect of rec-3 on polarity of recombination in the amination-1 locus of neurospora crassa. 19715553676
common regulation of recombination at the amination-1 and histidine-2 loci in neurospora crassa. 19715553663
sulphate transport by protoplasts of neurospora crassa. 19714270665
sugar transport in neurospora crassa. 3. an inositol requirement for the function of the glucose active transport system. 19715568189
molecular transport. i. in-vitro studies of isolated glycoprotein subunits of the amino acid transport system of neurospora crassa conidia. 19715569898
molecular transport. i. in vivo studies of transport mutants of neurospora crassa with altered amino acid competition patterns. 19715569897
mutagen specificity in neurospora crassa. 19715094801
some genetic and physiological characteristics of urease-defective strains of neurospora crassa. 19714938795
effects of mutations and growth conditions on lipid synthesis in neurospora crassa.a morphological mutant (col-2) of neurospora, which is partially deficient in glucose-6-phosphate dehydrogenase (g-6-pd) activity and has lower levels of reduced nicotinamide adenine dinucleotide phosphate (nadph), accumulated three-fold more triglycerides during log-phase growth than the wild-type strain. increased lipid deposition was not found in other strains that included slow-growing morphological mutants, nadph-deficient strains, g-6-pd-deficient mutants, wild-type revertants from col-2, ...19714400392
 in the extracts of quercus pedunculata roots, the activities of 5-dehydroquinate hydro-lyase and shikimate: nadp oxydoreductase are eluted in the same fractions whatever separation procedures are used: chromatography on sephadex g 100, deae cellulose and hydroxylapatite, centrifugation in sucrose density gradient, disc electrophoresis. these results are closely related to those obtained on the aggregate of enzymes involved in the shikimate pathway in neurospora crassa. the molecular weight of th ...197111945772
separation of mitochondrial membranes of neurospora crassa: i. localization of l-kynurenine-3-hydroxylase. 197119866780
regulation of sugar transport in neurospora crassa.sugar uptake systems in neurospora crassa are catabolically repressed by glucose. synthesis of a low k(m) glucose uptake system (system ii) in neurospora is derepressed during starvation for an externally supplied source of carbon and energy. fasting also results in the derepression of uptake systems for fructose, galactose, and lactose. in contrast to the repression observed when cells were grown on glucose, sucrose, or fructose, system ii was not repressed by growth on tryptone and casein hydr ...19715573733
kinetic characteristics of the two glucose transport systems in neurospora crassa.glucose is transported across the cell membrane of neurospora crassa by two physiologically and kinetically distinct transport systems. system ii is repressed by growth of the cells in 0.1 m glucose. system i is synthesized constitutively. the apparent k(m) for glucose uptake by system i and system ii are 25 and 0.04 mm, respectively. both uptake systems are temperature dependent, and are inhibited by nan(3) and 2,4-dinitrophenol. glucose uptake by system ii was not inhibited by fructose, galact ...19715573732
change in complementation patterns and enzyme activity of pyrimidine-3 mutants of neurospora crassa by induced reversion. 19715096222
identification of the genetic alteration at the molecular level of ultraviolet light-induced ad-3b mutants in neurospora crassa. 19715096221
hydroxylamine-induced purple adenine (ad-3) mutants in neurospora crassa. i. characterization of mutants by genetic tests. 19715096220
[isolation and partial characterization of a mitochondrially synthesized protein from neurospora crassa]. 19715092530
metabolic role, regulation of synthesis, cellular localization, and genetic control of the glyoxylate cycle enzymes in neurospora crassa.the glyoxylate shunt enzymes, isocitrate lyase and malate synthase, were present at high levels in mycelium grown on acetate as sole source of carbon, compared with mycelium grown on sucrose medium. the glyoxylate shunt activities were also elevated in mycelium grown on glutamate or casamino acids as sole source of carbon, and in amino acid-requiring auxotrophic mutants grown in sucrose medium containing limiting amounts of their required amino acid. under conditions of enhanced catabolite repre ...19715541006
synchronization of converging metabolic pathways: activation of the cystathionine gamma-synthase of neurospora crassa by methyltetrahydrofolate.methyltetrahydrofolate synchronizes the activities of the two branches of the pathway of methionine biosynthesis in neurospora crassa by serving as an essential activator of cystathionine gamma-synthase and antagonizing the feedback inhibition of this enzyme by s-adenosylmethionine. activation is specific for the methylated form of folate and increases with increasing glutamate content. the inability of extracts of me-1 and me-6 mutants to form cystathionine that has been previously reported is ...19715277076
defective production of mitochondrial ribosomes in the poky mutant of neurospora crassa.a strain of neurospora crassa containing a cytoplasmic mutation (poky) affecting mitochondrial function is shown to be deficient in small ribosomal subunits in the mitochondrion during the exponential growth phase. in the stationary growth phase, small subunits are more abundant and are present in mitochondrial ribosomal monomers. this change can be correlated with the return of mitochondrial cytochrome content to amounts approaching those of wild type mitochondria. the ribosomal defect shows an ...19715277071
a particulate fraction from neurospora crassa exhibiting aryl sulfatase activity. 19714323729
chemical and physical studies of neurospora crassa invertase. molecular weight, amino acid and carbohydrate composition, and quaternary structure. 19715539234
mutagenic activities of aflatoxin b 1 and g 1 in neurospora crassa. 19715564467
the genetics and biochemical characterization of sterility mutants in neurospora crassa. 19715564337
cysteine toxicity in neurospora crassa: the mechanism of counteraction by amino acids. 19714256680
some properties of the 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase isoenzymes from mutant strains of neurospora crassa. 19715550829
neurospora mutant deficient in tryptophanyl-transfer ribonucleic acid synthetase activity.a tryptophan auxotroph of neurospora crassa, trp-5, has been characterized as a mutant with a deficient tryptophanyl-transfer ribonucleic acid (trna) synthetase (ec 6.1.1.2) activity. when assayed by tryptophanyl-trna formation, extracts of the mutant have less than 5% of the wild-type specific activity. the adenosine triphosphate-pyrophosphate exchange activity is at about half the normal level. in the mutant derepressed levels of anthranilate synthetase and tryptophan synthetase were associate ...19715540999
partial enzyme aggregates formed by pleiotropic mutants in the arom gene cluster of neurospora crassa.molecular weights of enzymically active arom aggregates produced by pleiotropic and various arom-1 mutants of neurospora crassa have been estimated by sucrose density gradient centrifugation. in contrast to most single-gene mutants (which produce intact arom multienzyme aggregates of normal molecular weight- about 230,000), pleiotropic mutants that lack two or more of the five enzyme activities in the arom aggregate produce partial arom aggregates of molecular weights ranging from about 60,000 t ...19715276302
sites of biosynthesis of outer and inner membrane proteins of neurospora crassa mitochondria. 19715578606
saturated fatty acid requirer of neurospora crassa.dietary saturated fatty acids containing 12- to 18-carbon atoms satisfy growth requirements of neurospora crassa mutant cel (previously named ol; perkins et al., reference 11); unsaturated fatty acids are synthesized by direct desaturation when an appropriate saturate is available. odd-chain saturates, 15 carbons and 17 carbons long, satisfy the requirement, and elaidic acid (18:1 delta(9)trans) results in slow growth. oleic acid and other cis-unsaturated fatty acids do not satisfy growth requir ...19714323964
isolation and chemical properties of a repressible acid phosphatase in neurospora crassa. 19715545084
a derepressible active transport system for glucose in neurospora crassa. 19715545073
amino acid transport in neurospora crassa. iv. properties and regulation of a methionine transport system. 19715579132
the peptidyl-puromycin reaction with neurospora crassa ribosomes. the number of active ribosomes in different conditions of growth. 19715129217
conidiation in neurospora crassa. 19714325980
hydroxylamine-induced purple mutants (ad-3) in neurospora crassa. ii. identification of genetic alteration at the molecular level. 19714281762
[effect of 8-azaadenine on the accumulation of high-polymer inorganic polyphosphates in neurospora crassa]. 19714265841
demonstration of dihydro-orotate dehydrogenase in neurospora crassa hyphae with a cytochemical procedure. 19714106595
neurospora crassa ribosomes: effects of bound peptidyl-trna on dissociation into subunits and on phenylalanine polymerization at low mg 2+ concentration. 19714257627
in vitro mitochondrial complementation in neurospora crassa. 19715116521
temperature-dependent actinomycin d effect on rna synthesis during synchronous development in neurospora crassa. 19715116518
genetic control of the synthesis of repressible phosphatases in neurospora crassa. 19714332791
physical properties of glucose 6-phosphate dehydrogenase from neurospora crassa. 19714399599
in vivo synthesis, molecular weights, and proportions of mitochondrial proteins in neurospora crassa. 19715160418
active transport of l-aspartic acid in neurospora crassa. 19714258794
transfer ribonucleic acid methylases during the germination of neurospora crassa.transfer ribonucleic acid (trna) methylases were studied during the germination of spores in neurospora crassa. the total methylase capacity and base specific trna methylase activities were determined in extracts from cells harvested at various stages of germination. germinated conidia have a 65% higher methylase capacity than ungerminated conidia. three predominant methylase activities were found in the extracts, and the relative amount of each activity was different at the various stages. enzy ...19715122811
regulation of glutamate dehydrogenases in neurospora crassa as a response to carbohydrates and amino acids in the media. 19714334010
cycloheximide resistant incorporation of amino acids into a polypeptide of the cytochrome oxidase of neurospora crassa. 19714329217
[localization of 1,3-diphosphoglycerate: polyphosphate-phosphotransferase in neurospora crassa cells]. 19715124944
nh2-terminal methionine in nascent peptides from neurospora crassa. 19714925709
mitochondrial rna polymerase from neurospora crassa. 19714933004
transcription of mitochondrial dna in vitro from neurospora crassa. 19714938619
fusidic acid resistance of mitochondrial g factor from neurospora crassa. 19714942120
invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a neurospora mutant and a component of molybdenum-enzymes.an active neurospora-like assimilatory nadph-nitrate reductase (ec 1.6.6.2), which can be formed in vitro by incubation of extracts of nitrate-induced neurospora crassa mutant nit-1 with extracts of (a) certain other nonallelic nitrate reductase mutants, (b) uninduced wild type, or (c) xanthine oxidizing and liver aldehyde-oxidase systems was also formed by combination of the nit-1 extract with other acid-treated enzymes known to contain molybdenum. these molybdenum enzymes included (a) nitrogen ...19714399835
a nuclease from neurospora crassa conidia specific for single-stranded nucleic acids. 19714269245
isoprenoid phenol and quinone precursors of ubiguinones and dihydroubiguinones (ubiguinones (h 2 )) in fungi.1. ten moulds and two yeasts were analysed for the presence of 2-polyprenylphenols, 2-polyprenyl(h(2))phenols, 6-methoxy-2-polyprenylphenols, 6-methoxy-2-polyprenyl(h(2))phenols, 6-methoxy-2-polyprenyl-1,4-benzoquinones, 6-methoxy-2-polyprenyl(h(2))-1,4-benzoquinones, 5-demethoxyubiquinones, 5-demethoxyubiquinones(h(2)), ubiquinones and ubiquinones(h(2)). 2. the organisms were found to be of three types: (a) those that contained only ubiquinones (aspergillus fumigatus and penicillium brevi-compa ...19715166547
a structural gene for neurospora crassa isocitrate lyase. 19714996780
studies with purple adenine mutants in neurospora crassa. vi. the effects of differences in genetic background on ad-3a x ad-3b crosses. 19715127402
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