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particle weights of active ribosomal subunits from neurospora crassa. 19734268077
transport of c 4 -dicarboxylic acids in neurospora crassa. 19734268075
identification of sexual hormones in neurospora crassa. 19724267851
the ribonuclease activities of the single-strand-specific nucleases of neurospora crassa. 19734267583
an inhibitory factor affecting ribonuclease activity in neurospora crassa. 19734267553
adenine nucleotide levels in neurospora, as influenced by conditions of growth and by metabolic inhibitors.by a combination of luciferase and fluorescence methods adenine nucleotide pools in neurospora crassa have been examined under various conditions of growth and metabolic inhibition. during sustained exponential growth (25 c, shaking liquid cultures), the intracellular adenosine 5'-triphosphate (atp) concentration, [atp](i), rises slowly from the conidial level near 1 mm (1 mmol/kg of cell water) to a maximum of 2.0 to 2.5 mm at 14 h, after which it slowly declines. the adenosine 5'-diphosphate a ...19734267534
structure of the mitochondria of neurospora crassa as revealed by thin sectioning and freeze-etch techniques. 19724267202
dissociation of mitochondrial ribosomes of neurospora crassa by a bacterial dissociation factor. 19734266973
increased viability of a strain of neurospora crassa after treatment with ultraviolet radiation or other agents.a strain of neurospora crassa with low viability but near normal germination shows a two- to sixfold increase in the number of colonies formed after treatment with ultraviolet radiation, x radiation, nitrous acid, or heat (50 c). effective dosages of these agents produce moderate killing of the wild type.20144266244
histidine uptake in mutant strains of neurospora crassa via the general transport system for amino acids.a transport double mutant of neurospora crassa has been isolated that has only one of the three transport systems capable of l-histidine uptake. the substrate specificity of the remaining transport system, termed the general transport system, has been fully characterized with regard to the contributions to binding of the side chain, the alpha-amino group, and the carboxylate group. the positively charged alpha-amino group is necessary for binding; the negatively charged carboxylate group is of l ...19734266243
distinct kynureninase and hydroxykynureninase activities in microorganisms: occurrence and properties of a single physiologically discrete enzyme in yeast.(i) saccharomyces cerevisiae grown in the presence of 1.0 mm l-tryptophan slowly excreted fluorescent material that was chromatographically identifiable as 3-hydroxyanthranilate but did not excrete detectable amounts of anthranilate nor rapidly deplete the medium of l-tryptophan. under similar growth conditions, neurospora crassa rapidly excretes anthranilate and rapidly depletes the medium of l-tryptophan. (ii) chromatographic analysis of crude extracts from yeast revealed a single kynureninase ...19734266242
differential gene action in neurospora crassa.molecular hybridization experiments with pulse-labeled total ribonucleic acid (rna) or isolated nonribosomal rapidly labeled rna species and deoxyribonucleic acid from growth periods of 8, 16, and 24 hr of neurospora crassa showed differential transcription. hybridization competition experiments between rna species isolated from 8, 16, and 24 hr of growth showed qualitative differences in the types of rna synthesized during these periods.19734266171
life cycle of neurospora crassa viewed by scanning electron microscopy.scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of neurospora crassa shear and dodge (st. lawrence 3.1a and 74a): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and outgrowth of macroconidia. structures seen at the limit of resolution of bright-field and phase-contrast microscopes, e.g., the ribbed surface of ascospores, are well resolved. new de ...19734266170
preparation of mitochondrial membrane proteins from neurospora crassa: prevention of lipid autoxidation damage by an antioxidant. 20144266161
role of lysine and -n-trimethyllysine in carnitine biosynthesis. i. studies in neurospora crassa. 19734266139
the isolation of histone from neurospora crassa. 19734266136
conidial repression by phenethyl alcohol and its serological consequences in neurospora crassa. 19724265955
aryl sulfatase in ascospores of neurospora crassa.neurospora crassa ascospores normally do not contain aryl sulfatase even when formed under conditions of sulfur limitation. however, when one of the parental strains is the nonrepressible mutant scon(c), the resulting (mixed) ascospores contain significant levels of aryl sulfatase even when formed under conditions of sulfur abundance.19734265939
tryptophan transport in neurospora crassa by various types of mtr revertants. 19724265907
the effects of branched chain fatty acid incorporation into neurospora crassa membranes. 19724265873
[effect of 8-azaadenine on the accumulation of high-polymer inorganic polyphosphates in neurospora crassa]. 19714265841
sterol mutants of neurospora crassa: their isolation, growth characteristics and resistance to polyene antibiotics. 19734265660
the isolation of the components of the anthranilate synthetase complex from neurospora crassa. 19734265643
thymidine 2'-hydroxylation in neurospora crassa. 19724265566
genetic alterations at the molecular level in x-ray induced ad-3b mutants of neurospora crassa. 20144265447
alkaline phosphatases of neurospora crassa. 3. effects of ph and mechanism of action. 19734265429
adenylate cyclase activity in lubrol-treated membranes from neurospora crassa. 19724265406
[neurospora crassa pyruvate dehydrogenase: interconversion due to phosphorylation and dephosphorylation]. 19724265372
control of acetohydroxy acid synthetase in neurospora crassa. 19724265245
[metabolic products of microorganisms. 115. uptake of iron by neurospora crassa. i. to the specificity of iron transport]. 19734265135
delta-aminolaevulinate dehydratase, the regulatory enzyme of the haem-biosynthetic pathway in neurospora crassa.the activity of delta-aminolaevulinate dehydratase is very low in the mould neurospora crassa compared with the activities detected in bacterial and animal systems. the enzyme is inducible in iron-deficient cultures by addition of iron and is repressed by protoporphyrin. the properties of the purified enzyme indicate its allosteric nature and susceptibility to feedback inhibition by coproporphyrinogen iii. neurospora extracts also contain a protein inhibitor of the enzyme and a small-molecule ac ...19724265023
genetic and metabolic control of sulfate metabolism in neurospora crassa: a specific permease for choline-o-sulfate. 19724265021
direct induction in wild-type neurospora crassa of mutants (qa-1 c ) constitutive for the catabolism of quinate and shikimate.a color test has been developed for the selection and identification of mutants in neurospora crassa, constitutive for the three normally inducible enzymes which convert quinate to protocatechuate. by this means seven such mutants have been recovered after ultra violet irradiation of wild type and have been shown to be allelic (or very closely linked) to the qa-1(c) mutants previously obtained by other means. thus, the regulation of the synthesis of these three catabolic enzymes is indicated to ...19724264707
induction of mitochondrial rna polymerase in neurospora crassa. 19724264635
effect of 8-azaguanine on polyribosomes of neurospora crassa. 19724264601
a possible causal relationship between iron deficiency, inhibition of dna synthesis and reduction of meiotic recombination frequency in neurospora crassa. 19724264533
mutagenicity of chemical carcinogens in neurospora crassa. 19724264527
the kinetics of l-aspartate transport in neurospora crassa conidia. 19724264473
cytochrome b in neurospora crassa mitochondria. a membrane protein containing subunits of cytoplasmic and mitochondrial origin. 19724264399
permeability problems encountered when treating conidia of neurospora crassa with rna synthesis inhibitors. 19724264155
interactions of phenylalanyl transfer ribonucleic acid synthetase of neurospora crassa with valyl transfer ribonucleic acid of escherichia coli. 19724264131
[isolation and properties of plasma membranes of neurospora crassa mycelium]. 20144264117
ribosomal rna genes of neurospora: isolation and characterization.dna sequences in neurospora crassa that code for ribosomal rna have been isolated in a highly purified state by dna-rna hybridization. about 100 separate ribosomal rna genes exist in each nucleus. these genes are composed of repeated sequences that are present about 100 times per nucleus. the individual members of this group of dna sequences are essentially identical.19724264050
purification and stability of the multienzyme complex encoded in the arom gene cluster of neurospora crassa. 19724263953
an inhibitor of ribonucleic acid polymerase from neurospora crassa. 19724263858
the effect of thiourea on ureide metabolism in neurospora crassa. 19724263857
the regulation of nitrate reductase in neurospora crassa. 19724263853
adenylate cyclase activity in neurospora crassa. ii. kinetics. 19724263616
activation of membrane-bound adenylate cyclase by glucagon in neurospora crassa.membrane-bound adenylate cyclase in neurospora crassa is activated by glucagon. half-maximal effect is observed at hormone concentrations of about 10 nm. after solubilization of the enzyme with lubrol-px, the glucagon effect is lost. incubation of neurospora cells with glucagon leads to a decrease in the activity of glycogen synthetase (ec 2.4.1.11) and to an increase in the activity of glycogen phosphorylase (ec 2.4.1.1) and in the rate of glycogenolysis.20144263508
uncoupling of the recbc atpase from dnase by dna crosslinked with psoralen.exonucleolytic cleavage of dna by the recbc dnase is accompained by a dna-dependent atp hydrolysis that ceases when the dna that has been digested to a limit. on the other hand, dna that has been crosslinked by 4,5',8-trimethylpsoralen in the presence of 360-nm light remains an effective cofactor in the atpase reaction, but is resistant to digestion by the enzyme. psoralentreated dna is degraded by pancreatic dnase, micrococcal nuclease, and escherichia coli b restriction enzyme, but not by neur ...19724263506
interaction of genes controlling ultraviolet sensitivity in neurospora crassa.two independently segregating ultraviolet (uv) sensitivity genes in neurospora crassa interact synergistically resulting in uv sensitivity approximately twice that expected based on an evaluation of the sensitivities of the individual mutants. the mutant genes singly and together reduce photoreactivation (pr) in vivo although a pr enzyme is produced which exhibits normal activity in vitro.19724263406
acetohydroxy acid synthetase with a ph optimum of 7.5 from neurospora crassa mitochondria: characterization and partial purification.an acetohydroxy acid synthetase (aas) has been found associated with the mitochondrial fraction of wild-type neurospora crassa. it has a ph optimum of 7.5 and is presumed to be homologous to the ph 8.0 aas that synthesizes the valine and isoleucine precursors in bacteria and yeast. the enzyme was characterized and purified 30- to 60-fold. the aas activity of intact mitochondria requires thiamine pyrophosphate (tpp), mn(2+) or mg(2+), and flavine adenine dinucleotide (fad), and is sensitive to en ...19724263405
interactions between amino acid transport systems in neurospora crassa.mutants of neurospora crassa, selected as resistant to l-canavanine and l-thialysine, are partially deficient in the uptake of basic amino acids. neutral amino acids completely inhibit uptake of basic amino acids, and this inhibition is dependent on the activity of a neutral amino acid permease. in contradistinction, mutants resistant to 4-methyl-dl-tryptophan are partially deficient in the uptake of neutral amino acids. basic amino acids completely inhibit neutral amino acid uptake, and this in ...19724263403
the effect of phosphate buffer on the differential response of two genes in neurospora crassa to uv. 20144263301
[conidial differentiation of neurospora crassa. 3. malate dehydrogenase activity of antigenic structures and its relation to conidial competence]. 19724263229
sexuality in neurospora crassa. ii. genes affecting the sexual development cycle. 19724263084
sexuality in neurospora crassa. i. mutations to male sterility. 20144263083
enzymic properties of a mutant tryptophan synthase from neurospora crassa. 19724262962
mutagenic activity of 4-nitroquinoline 1-oxide and 4-hydroxyaminoquinoline 1-oxide in neurospora crassa. 19724262705
complementation among cytoplasmic mutants of neurospora crassa. 19724262636
analysis of a case of mutagen specificity in neurospora crassa. iv. interactions between uv and three chemical mutagens. 19724262635
[nucleus directed biosynthesis of the mitochondrial genetic apparatus in neurospora crassa]. 19724262620
development of fertile fruit bodies in a single-strain culture of neurospora crassa. 19724262618
isolation and classification of extranuclear mutants of neurospora crassa.four extranuclear mutants, [exn-1], [exn-2],[exn-4], and [stp-c], were obtained from n-methyl-n'-nitro-n-nitrosoguanidine-treated conidia and mycelium of neurospora crassa. the three exn mutants grow with a pronounced lag from conidia and ascospores and are female fertile, whereas [stp-c] has a stop-start growth phenotype and is female sterile. the mitochondria from all four mutants are deficient in cytochromes a+a(3) and b, but contain an excess of cytochrome c. on the basis of growth and ferti ...19724262391
cell wall alterations associated with the hyperproduction of extracellular enzymes in neurospora crassa.a pleiotropic mutation in neurospora (exo-1), which confers derepression of alpha-amylase, glucoamylase, beta-fructofuranosidase, and trehalase, appears to also affect the composition of the cell wall. segregants resulting from the backcross of exo-1 to the wild-type strain from which it derived are altered in the ratio of galactosamine to glucosamine in hydrolysates of isolated cell walls. conidial cell walls exhibit a marked decrease in the amount of galactosamine in both exo-1 and exo-1(+) st ...19724262302
altered species of mitochondrial transfer rna associated with the mi-1 cytoplasmic mutation in neurospora crassa. 19724262272
pyruvate kinase of neurospora crassa: effect of various ligands on the rate of inactivation by protein denaturants. 19724262270
[study on the conidial differentiation of neurospora crassa. ii. immunological comparison of conidiogenic wild-type antigenic structures with those of 2 aconidial mutants]. 19724262114
mutation induction in neurospora crassa incubated in mice and rats. 19724261732
dominance modifiers in neurospora crassa: phenocopy selection and influence of certain ascus mutants.when homozygous in zygotes, mutant alleles at the peak locus in linkage group v of neurospora crassa initiate aberrant asci that are nonlinear, in contrast to the linear asci characteristic of wild type. most mutant alleles are recessive, inasmuch as crosses of the mutant strains with wild type give linear asci. however, five different mutant alleles, when heterozygous with the wild-type allele, act in varying degrees as zygote dominants, initiating both linear and nonlinear asci, the relative p ...19724261623
genetic and enzymatic analysis of a gene controlling uv sensitivity in neurospora crassa. 19724261527
purification of two multienzyme complexes in the aromatic-tryptophan pathway of neurospora crassa. 19724261463
control of the synthesis, activity, and turnover of enzymes of sulfur metabolism in neurospora crassa. 19724261415
genes influencing selective fertilization in neurospora crassa.the mutual attraction of conidia to protoperithecia of the opposite mating type was studied genetically in crosses where a mixture of conidia from two different strains, one of which was marked by an ascospore color mutant gene tan spore (ts), was applied to protoperithecia. selective fertilization was measured as the frequency of perithecia fertilized by conidia from one strain in competition with conidia from another strain. selective fertilization by a given strain varied throughout the range ...19724261371
isolation of transfer rna genes from neurospora crassa. 19724261112
electron microscopy of nuclear membranes during somatic nuclear separation in neurospora crassa. 19724261058
the intracellular site of synthesis of mitochndrial ribosomal proteins in neurospora crassa.the intracellular site of synthesis of mitochondrial ribosomal proteins (mrp) in neurospora crassa has been investigated using three complementary approaches. (a) mitochondrial protein synthesis in vitro: tritium-labeled proteins made by isolated mitochondria were compared to (14)c-labeled marker mrp by cofractionation in a two-step procedure involving isoelectric focusing and polyacrylamide gel electrophoresis. examination of the electrophoretic profiles showed that essentially none of the peak ...19724261038
genetical and biochemical evidence for further interrelationships between the polyaromatic synthetic and the quinate-shikimate catabolic pathways in neurospora crassa. 19724261015
cooperation of mitochondrial and nuclear genes specifying the mitochondrial genetic apparatus in neurospora crassa.enzymes involved in the expression of the mitochondrial genome in neurospora crassa are induced by chloramphenicol and ethidium bromide, which block transcription and translation of mitochondrial dna. it is concluded that most, if not all, proteins of the mitochondrial genetic apparatus are coded by nuclear genes, synthesized on cytoplasmic ribosomes, and controlled by a repressor-like mitochondrial gene product. a model explaining the coordination of nuclear and mitochondrial division cycles by ...19724260903
pyruvate kinase of neurospora crassa: purification and some properties. 20144260764
genetic characterization of diethylnitrosamine-induced purple adenine (ad-3) mutants in neurospora crassa. 19724260579
regulation of arginase activity by intermediates of the arginine biosynthetic pathway in neurospora crassa.it has been found that, in neurospora crassa, arginine synthesized from exogenous citrulline was not as effectively hydrolyzed as exogenous arginine. this was explained by the observed inhibition of arginase in vitro and in vivo by citrulline. the high arginine pool formed from exogenous citrulline feedback inhibits the arginine pathway. these two factors allow exogenous citrulline to be used adventitiously and efficiently as an arginine source. finally, it was found that ornithine was a strong ...19724260560
regulation of exocellular proteases in neurospora crassa: induction and repression of enzyme synthesis.neurospora crassa strain 74a grown on vogel's medium containing bovine serum albumin (bsa) as principal carbon source secretes proteolytic enzymes which appear in the culture filtrate. low concentrations of sucrose (0.1%) are necessary for growth from conidia, as conidia will not germinate on bsa alone. once growth is initiated, however, protease production begins and at 5 to 6 hr growth and enzyme production are parallel. higher concentrations of sucrose (0.5-2%) repress protease synthesis. oth ...19724260559
simple and reliable method for replica plating neurospora crassa.the details of a simple method for replica plating neurospora crassa are described. the procedure has proved to be highly reliable for replica plating of colonies arising from either ascospores or conidia.19724260558
repair of ultraviolet light-induced damage to the deoxyribonucleic acid of neurospora crassa.a method is described for labeling a specific pyrimidine in the deoxyribonucleic acid (dna) of neurospora crassa. in cells grown in the presence of [5-(3)h]-uridine, more than 97% of the radioactivity associated with the dna had been incorporated into cytosine. the specific activity of the labeled dna was approximately 3 x 10(3) counts per min per mug. the dna was isolated by elution from hydroxyapatite columns with sodium phosphate buffer (0.40 m, ph 6.8). this procedure was used to demonstrate ...19724260557
circular dna from mitochondria of neurospora crassa. 19724260513
arginyl-transfer ribonucleic acid and synthetase of neurospora crassa. 20134260497
the separation of three allosterically inhibitable 3-deoxy-d-arabino-heptulosonate 7-phosphate synthases from extracts of neurospora crassa and the purification of the tyrosine inhibitable isoenzyme. 19724260319
super suppressors in neurospora crassa i. induction, genetic localization and relationship to a missense suppressor.genetic analyses have been made to test the feasibility of using coincident reversions to prototrophy of multiple mutants to select super suppressors (ssu) in neurospora crassa. of five double-mutant strains examined, only those mutant combinations in which both members had the properties of nonsense mutations did revert coincidently. forty-eight genetically purified coincident revertants were crossed to the wild type, and each was shown to contain a suppressor mutation. five super suppressors w ...19724260182
properties of two nuclease genes in neurospora crassa.two genes, nuc-1 and nuc-2, of neurospora crassa which were responsible for the nucleic acid digestion, were located on linkage group 1 and 2, respectively. a temperature-sensitive mutant (b1ts-2) was obtained from a nuc-2 mutant. nuclease mutants showed a reduced activity of nuclease n(3) which was found to be a complex consisting of nuclease n(3) (') and inhibitor molecule. nuclease n(3), nuclease n(3) (') and inhibitor were partially purified and estimated to have the approximate molecular we ...20144260181
sugar transport in neurospora crassa. 19724260082
regulation of nitrate reductase in neurospora crassa: regulation of transcription and translation.a technique employing cycloheximide and actinomycin d has been used for the separation of transcription and translation during the induction of nitrate reductase in neurospora crassa. nitrate reductase is found to be synthesized in low efficiency when nitrate is not provided during both transcription and translation. nitrate reductase synthesis is enhanced by nitrate. nitrate is found to induce nitrate reductase by enhancing the increase of the capacity to synthesize nitrate reductase, and ammon ...19724259976
alkaline phosphatases of neurospora crassa. ii. product inhibition studies. 19724259964
an unlinked mutation affecting control of purine metabolism in a revertant of an ad-7 auxotroph of neurospora crassa lacking the phosphoribosylpyrophosphate amidotransferase. 19724259695
genes conferring resistance to 8-aza adenine in neurospora crassa and the variability of resistant alleles in the aza-1 locus with respect to excretion of purines. 19724259694
histidine uptake in strains of neurospora crassa with normal and mutant transport systems.kinetic parameters for three systems of active histidine uptake by germinated conidia of neurospora crassa have been measured. each system appears to follow typical michaelis-menten kinetics when studied separately from the other systems. under the conditions studied, the general amino acid transport system was found to account for the major portion of histidine uptake from low concentrations. three types of transport mutants with altered growth inhibition patterns were selected in a histidine a ...19724259665
inhibition of tryptophan synthetase by indoleacrylic acid.indoleacrylic acid (5 x 10(-4)m in the growth medium) inhibits the growth of mycelia of neurospora crassa and causes the cells to accumulate indoleglycerol phosphate. measurement of kinetic parameters of partially purified tryptophan synthetase in the presence of indoleacrylic acid showed the following patterns of inhibition: in reaction 1, the physiological reaction, indoleglycerol phosphate + serine [formula: see text] tryptophan + glyceraldehyde-3-phosphate (unusual with respect to indoleglyc ...19724259663
detection of circular dna from mitochondria of neurospora crassa. 19724259385
dihydroorotate-quinone reductase of neurospora crassa mitochondria. 19714259267
separation of mitochondrial membranes of neurospora crassa. ii. submitochondrial localization of the isoleucine-valine biosynthetic pathway.separation of neurospora mitochondrial outer membranes from the inner membrane/matrix fraction was effected by digitonin treatment and discontinuous density gradient centrifugation. the solubilization of four isoleucine-valine biosynthetic enzymes was studied as a function of digitonin concentration and time of incubation in the detergent. the kinetics of the appearance of valine biosynthetic function in fractions outside of the inner membrane/matrix fraction, coupled with enzyme solubilization ...19724259051
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