PMID(sorted descending)
comparative regulation of isoenzymic 3-deoxy-d-arabino-heptulosonate 7-phosphate synthetases in microorganisms.the independent control of regulatory isoenzymes by different metabolites constitutes one well-known pattern of control in branched metabolic pathways. this pattern was previously found to be widely distributed in the aromatic amino acid pathway of microorganisms in the case of the first enzyme of the sequence, 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthetase. the comparative stability of the isoenzymes as well as the effect of aromatic amino acids in the growth medium upon the leve ...19684867215
the manipulation of mutation-induction kinetics in neurospora crassa. 19734726377
circular dichroism of a membrane protein of neurospora crassa. 19734703556
enzymatic hydrolysis of penicillin g and other phenylacetylamino compounds by neurospora crassa acylase. 19734693635
depolarization of the plasma membrane of neurospora during active transport of glucose: evidence for a proton-dependent cotransport system.intracellular microelectrodes were used to measure the effects of glucose transport on membrane voltage and membrane resistance in neurospora crassa. sudden activation of glucose uptake, via the high-affinity, derepressible system ii, results in a very large depolarization of the plasma membrane. at saturating concentrations of glucose, the depolarization averages 120 mv; it is diphasic in time, with an initial shift at rates of 100-200 mv/sec followed by a slow, spontaneous, partial repolarizat ...19744525303
protoperithecia in neurospora crassa: technique for studying their development.a technique is described which facilitates analysis of the development of individual protoperithecia in neurospora crassa. the formation of this organelle proceeds in several clearly discernible steps beginning with the looping of a single hyphal filament and ending with a heavily pigmented, densely packed structure that is the mature protoperithecial structure. the effects of a number of environmental conditions on the development of protoperithecia in two wild types and in a female-sterile mut ...19664410944
regulation of pyridine nucleotide levels and ratios in neurospora crassa. 19724405599
[the isolation and characterization of a nadh: semidehydroascorbic acid oxidoreductase from neurospora crassa]. 19724405497
formation and reduction of intermediate acyladenylate by aryl-aldehyde. nadp oxidoreductase from neurospora crassa. 19724405494
[reduction of aromatic acids to aldehydes and alcohols using enzymes from neurospora crassa]. 19724405469
protein fluorescence of nicotinamide nucleotide-dependent dehydrogenases.1. the decrease in the protein fluorescence (f) of neurospora crassa glutamate dehydrogenase is linearly related to the increase in the fraction of the coenzyme sites occupied by nadph (alpha) at ph6.35. under these conditions nadph causes this enzyme to dissociate to monomers. 2. there is a non-linear relationship of f to alpha for nadh binding to give the alcohol dehydrogenase-nadh-isobutyramide complex, the l-glycerol 3-phosphate dehydrogenase-nadh complex and the bovine glutamate dehydrogena ...19724404769
the subunit structure of the nicotinamide-adenine dinucleotide phosphate-dependent glutamate dehydrogenase of neurospora crassa. 20154403952
studies on the glutamate dehydrogenase from neurospora crassa. 20154403951
synthesis of amino acids from succinate by the mitochondria of neurospora crassa. 19724403712
regulation of the two "malic" enzymes in neurospora crassa. 19724402367
the induction of nitrite reductase in neurospora crassa. 19724402141
regulation of nitrate reductase in neurospora crassa: stability in vivo.nicotinamide adenine dinucleotide phosphate, reduced form (nadph)-nitrate reductase and its related enzyme activities, nadph-cytochrome c reductase and reduced benzyl viologen-nitrate reductase, are all induced following the transfer of ammonia-grown wild-type neurospora mycelia to nitrate medium. after nitrate reductase is induced to the maximal level, the addition of an ammonium salt to, or the removal of nitrate from, the cultures results in a rapid inactivation of nitrate reductase and its t ...20154401813
the electron transport components of wild type and poky strains of neurospora crassa. 19724401060
effects of mutations and growth conditions on lipid synthesis in neurospora crassa.a morphological mutant (col-2) of neurospora, which is partially deficient in glucose-6-phosphate dehydrogenase (g-6-pd) activity and has lower levels of reduced nicotinamide adenine dinucleotide phosphate (nadph), accumulated three-fold more triglycerides during log-phase growth than the wild-type strain. increased lipid deposition was not found in other strains that included slow-growing morphological mutants, nadph-deficient strains, g-6-pd-deficient mutants, wild-type revertants from col-2, ...19714400392
the amino acid composition and some properties of the nad + -specific glutamate dehydrogenase from neurospora crassa. 19714399934
invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a neurospora mutant and a component of active neurospora-like assimilatory nadph-nitrate reductase (ec, which can be formed in vitro by incubation of extracts of nitrate-induced neurospora crassa mutant nit-1 with extracts of (a) certain other nonallelic nitrate reductase mutants, (b) uninduced wild type, or (c) xanthine oxidizing and liver aldehyde-oxidase systems was also formed by combination of the nit-1 extract with other acid-treated enzymes known to contain molybdenum. these molybdenum enzymes included (a) nitrogen ...19714399835
physical properties of glucose 6-phosphate dehydrogenase from neurospora crassa. 19714399599
allosteric interaction of d-glutamate with the glutamate dehydrogenases of neurospora crassa. 19704398827
genetic and biochemical studies of the hexose monophosphate shunt in neurospora crassa. ii. characterization of biochemical defects of the morphological mutants colonial 2 and colonial 3. 19714397247
genetic and biochemical studies of the hexose monophosphate shunt in neurospora crassa. i. the influence of genetic defects in the pathway on colonial morphology. 19714397246
altered fatty acid distribution in mutants of neurospora crassa.morphological mutants of neurospora with decreased levels of reduced nicotinamide adenine dinucleotide phosphate (nadph) and reduced nicotinamide ad enine dinucleotide (nadh) contained only 20% as much of a polyunsaturated fatty acid (linolenic acid) as the wild type in both the phospholipid and neutral lipid fractions. there was an excellent correlation between linolenic acid levels and morphological appearance as a function of total nadph content, but no correlation with nadh content. the lino ...19704395199
characterization of neurospora crassa mitochondria prepared with a grind-mill. 19704392865
role of molybdenum in the regulation of nitrate reductase in neurospora crassa. 19694392507
correlation between reduced nicotinamide adenine dinucleotide phosphate levels and morphological changes in neurospora crassa.a colonial mutant of neurospora crassa, previously shown to be altered in the structure of glucose-6-p dehydrogenase [a reduced nicotinamide adenine dinucleotide phosphate (nadph) producing reaction], contained only 40% as much nadph in extracts as did the wild type. a partial revertant strain, when grown at 23 c, had the same total nadph content as the wild type, but, at 34 c, had lower levels of nadph as well as a colonial morphology. a revertant with complete wild-type morphology had wild-typ ...19704392398
catabolite-controlled regulation of glutamate dehydrogenases of neurospora crassa. 19704391972
formation of assimilatory nitrate reductase by in vitro inter-cistronic complementation in neurospora vitro complementation of the soluble assimilatory nicotinamide adenine dinucleotide phosphate, reduced form (nadph)-nitrate reductase was attained by mixing cell-free preparations of certain neurospora nitrate reductase mutants: induced nit-1 (uniquely possessing inducible nadph-cytochrome c reductase) with (a) uninduced or induced nit-2 or nit-3, or (b) uninduced wild type. the complementing activity of induced nit-1 is soluble while that of nit-2, nit-3, and wild type is particulate but not ...20154391854
enzymatic properties of l-kynurenine 3-hydroxylase (ec in neurospora crassa. 19704391570
[effect of some orotaldehyde derivatives as orotic acid antagonists in neurospora crassa]. 20144391358
enzyme activities during the asexual cycle of neurospora crassa. 3. nicotinamide adenosine diphosphate glycohydrolase. 19694391099
particulate enzymes of the glyoxylate cycle in neurospora crassa. 19694390938
the amino acid composition of the subunit of the nadp-specific glutamate dehydrogenase from neurospora crassa. 19694390421
[reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 2. purification and properties of aryl-alcohol: nadp-oxidoreductase from neurospora crassa]. 19694389864
[reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 1. purification and properties of aryl-aldehyde: nadp-oxidoreductase from neurospora crassa]. 19694389863
the formation of 5-formyluracil by cell-free preparations from neurospora crassa. 19684387266
"no-growth"-complementation in forced heterokaryons from sorbose-resistant (transport-defective) neurospora crassa mutants. 19674385815
[uptake of the sugars sorbose, fructose and glucose by sorbose-resistant mutants of neurospora crassa]. 19674384839
[active uptake of sugars by cells of neurospora crassa in the presence of an enzymatic system with permease properties ii]. 19674384716
[active uptake of sugars by cells of neurospora crassa in the presence of an enzymatic system with permease properties i]. 19674384715
enzyme activities during the asexual cycle of neurospora crassa. ii. nad- and nadp-dependent glutamic dehydrogenases and nicotinamide adenine dinucleotidase.three enzymes, (a) nicotinamide adenine diphosphate-dependent glutamic dehydrogenase (nad enzyme), (b) nictoinamide adenine triphosphate-dependent glutamic dehydrogenase (nadp enzyme), and (c) nicotinamide-adenine dinucleotidase (nadase), were measured in separate extracts of neurospora crassa grown in vogel's medium n and medium n + glutamate. specific activities and total units per culture of each enzyme were determined at nine separate intervals phased throughout the asexual cycle. the separa ...19684384627
involvement of a b-type cytochrome in the assimilatory nitrate reductase of neurospora crassa. 19674384346
regulation of the "malic" enzyme in neurospora crassa. 19674383262
inositol biosynthesis in neurospora crassa. 19674383057
studies of a homoserineless bradytroph of neurospora crassa: demonstration of an altered aspartate beta-semialdehyde dehydrogenase. 19674383000
the primary biochemical effect of a morphological mutation in neurospora crassa. 19664381935
changes in enzymes regulating isocitrate breakdown in neurospora crassa. 19654379827
proceedings: precursors of cytochrome oxidase in cytochrome-oxidase deficient cells of neurospora crassa. 19744376789
cyclic amp-induced tyrosinase synthesis in neurospora crassa. 20154376002
precursors of cytochrome oxidase in cytochrome-oxidase-deficient cells of neurospora crassa. comparison of the nuclear mutant cni-1, the cytoplasmic mutant mi-1, and copper-depleted wild type. 19744374355
the effect of essential amino acid deprivation on macromolecular synthesis and nucleotide pool sizes in neurospora crassa. 19744374128
rhythms of enzyme activity associated with circadian conidiation in neurospora crassa.the mycelial growth front of the band strain of neurospora grown on a solid surface exhibits a circadian rhythm of conidiation. enzyme assays on extracts from that mycelium have shown that the activities of 6 of 13 enzymes (nicotinamide adenine dinucleotide nucleosidase, isocitrate lyase, citrate synthase, glyceraldehydephosphate dehydrogenase, phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase) and soluble-protein content oscillate with the visible morphological change. the r ...19744373437
requirement for specific carbon sources in the low temperature induction of glycerol kinase in neurospora crassa. 19744373295
hyphal tips of wild-type and spreading colonial mutants of neurospora crassa. 19744372967
expression of the mitochondria genome in wild type and in extracellular mutants of neurospora crassa. 19734372151
revertants and secondary arom-2 mutants induced in non-complementing mutants in the arom gene cluster of neurospora crassa.extensive genetical and biochemical studies have been performed with revertants and secondary arom-2 mutants induced in two different primary non-complementing mutants which map within the arom gene cluster of neurospora crassa. these studies indicate that mutant m54 but not m25 can revert by super-suppressor mutations in unlinked genes, thus confirming previous evidence that m54 contains a nonsense codon. at least three new super suppressors of m54 have been detected. all four super suppressors ...19744371577
cross-pathway regulation: tryptophan-mediated control of histidine and arginine biosynthetic enzymes in neurospora neurospora crassa, the starvation of tryptophan mutants for tryptophan resulted in the derepression of tryptophan, histidine, and arginine biosynthetic enzymes. this tryptophan-mediated derepression of histidine and arginine biosynthetic enzymes occurred despite the fact that the tryptophan-starved cells had a higher intracellular concentration of histidine and arginine than did nonstarved cells.19744368541
cross-pathway regulation: histidine-mediated control of histidine, tryptophan, and arginine biosynthetic enzymes in neurospora neurospora crassa, histidine starvation of histidine mutants resulted in derepression of histidine, tryptophan, and arginine biosynthetic enzymes. the same tripartite derepression occurred in wild-type strain 74a when it was grown in medium supplemented with 3-amino-1,2,4-triazole, an inhibitor of histidine biosynthesis. histidine-mediated derepression of tryptophan and arginine biosynthetic enzymes was not due to a lowered intracellular concentration of tryptophan or arginine, respectively. ...19744368540
the efficacy of various mutagens and polyene antibiotics for the induction and isolation of sterol mutants of neurospora crassa. 19744366322
proteinaceous hexagonal inclusions in hyphae of whetzelinia sclerotiorum and neurospora crassa. 19744366209
the biogenesis of mitochondria in neurospora crassa: ultrastructural changes induced by nutrients. 19744365709
a neurospora crassa morphological mutant showing reduced adenylate cyclase activity. 19744365561
temperature-sensitive pleiotropic revertants from a mutant in the arom gene cluster of neurospora crassa.genetical and biochemical studies have been performed with revertants induced in a polyaromatic mutant (no. 58) in the arom gene cluster of neurospora crassa. in addition to complete and partial revertants able to grow on minimal at both 25 degrees and 35 degrees , temperature-sensitive revertants capable of growth on minimal at 25 degrees but not at 35 degrees have been recovered. one of these revertants has been shown to lack biosynthetic dehydroquinase activity at both temperatures (utilizing ...19744365170
isolation and characterization of an unsaponifiable lipid from neurospora crassa. 20154364989
the cytidine mechanism and methylation pathway in the formation of n-methylated ethanolamine phosphoglycerides in neurospora crassa. 19744364967
the isolation and partial characterization of two novel sphingolipids from neurospora crassa: di(inositolphosphoryl)ceramide and ((gal)3glu)ceramide. 19744364652
3-hydroxy-3-methylglutaryl coa reductase and mevalonate kinase of neurospora crassa.two enzymes of polyisoprenoid synthesis, 3-hydroxy-3-methylglutaryl coenzyme a (hmg coa) reductase (mevalonate:nadp oxidoreductase [acylating coa], ec and mevalonate kinase (atp:mevalonate 5-phosphotransferase, ec, are present in the microsomal and soluble fractions of neurospora crassa, respectively. hmg coa reductase specifically uses nadph as reductant and has a k(m) for dl-hmg coa of 30 micro m. the activities of hmg coa reductase and mevalonate kinase are low in conidia ...19744363966
neurospora crassa and humicola lanuginosa cytochromes c: more homology in the heme region. 19744363050
neurospora crassa protein kinase. purification, properties, and kinetic mechanism. 19744362679
the relationship between atp and an electrogenic pump in the plasma membrane of neurospora crassa. 19734360924
the suppression of ad-3b mutants by supersuppressors in neurospora crassa.the action of eight suppersuppressors has been tested on 76 ad-3b mutants which were induced by base-pair transition mutagens. thirteen mutants were found to be suppressible by at least one ssu gene. most of the suppressible mutants were found to belong to a class in which one would expect to find nonsense mutants (polar complementing or noncomplementing classes, with at at the mutant site). however, suppression was observed in other classes, including those containing presumed missense mutants. ...19734360386
studies of respiratory components and oxidative phosphorylation in mitochondria of mi-1 neurospora crassa.oxidative phosphorylation has been demonstrated with mitochondria of the mi-1 respiratory mutant of neurospora crassa. the p/o ratios observed with these mitochondria were approximately 0.8 with citrate and 0.4 with either externally added reduced nicotinamide adenine dinucleotide (nadh), succinate, or ascorbate-tetramethyl-p-phenylenediamine (tpd). these p/o ratios suggest that there are only two sites of phosphorylation in mitochondria isolated from young (20 to 24 h) cultures of the mi-1 muta ...19744359654
ethylene glycol-induced alteration of conidial germination in neurospora nutrient medium containing 3.22 m ethylene glycol or glycerol, conidia of neurospora crassa grow as single cells, without forming the germ tubes characteristic of normal morphological germination. ethylene glycol is more effective than glycerol in producing this response. after growth in ethylene glycol medium for a suitable time, the cells are easily disrupted by an abrupt decrease in osmotic pressure. osmotic disruption yields intact nuclei and mitochondria, although mitochondrial fractions ...19744359649
products of mitochondrial protein synthesis in neurospora crassa. determination of equimolar amounts of three products in cytochrome oxidase on the basis of amino-acid analysis. 19734359388
membrane permeability and the loss of germination factor from neurospora crassa at low water activities.neurospora crassa conidia incubating in buffer at low water activities (a(w)) release a germination-essential component as well as 260-nm absorbing and ninhydrin-positive materials, regardless of whether an electrolyte or non-electrolyte is used to reduce a(w). chloroform and antibiotics known to increase cell-membrane permeability have a similar effect. this suggests that membrane damage occurs in media of low a(w) and that an increase in permeability is responsible for the release of cellular ...19744358044
infectious dna from herpes simplex virus: infectivity of double-stranded and single-stranded molecules.the infectious units in native and alkalidenatured preparations of dna of herpes simplex virus were characterized with respect to their sensitivity to neurospora crassa endonuclease, their sedimentation properties in high-salt, neutral sucrose gradients, and their sensitivity to hydrodynamic shearing forces. infectious molecules in native preparations were resistant to n. crassa endonuclease, sedimented at 56 s, and were highly sensitive to shearing forces. after alkaline denaturation, infectiou ...19734357884
chemically induced reversion and suppression of methionine-i alleles of neurospora crassa. 20154357302
mitochondrial protein synthesis and cyanide-resistant respiration in copper-depleted, cytochrome oxidase-deficient neurospora crassa. 19734356494
altered mitochondrial respiration in a chromosomal mutant of neurospora crassa.a mutant of neurospora crassa (cni-1) has been isolated that has two pathways of mitochondrial respiration. one pathway is sensitive to cyanide and antimycin a, the other is sensitive only to salicyl hydroxamic acid. respiration can proceed through either pathway and both pathways together in this mutant account for greater than 90% of all mitochondrial respiration. the cni-1 mutation segregates as a nuclear gene in crosses to other strains of neurospora. absorption spectra of isolated mitochond ...19734355489
structure and plugging of septa of wild type and spreading colonial mutants of neurospora crassa. 19734354924
electron microscopy of selectively stimulated microconidiogenesis in wild type neurospora crassa. 19734354923
cyclic 3',5'-amp phosphodiesterase of neurospora crassa. 19734354445
neurospora crassa pyruvate dehydrogenase: interconversion by phosphorylation and dephosphorylation. 19724352021
alternative methods of preparation and analysis of quasicrystalline protein from neurospora crassa. 19734351946
enzymatic properties of a repressible acid phosphatase in neurospora crassa. 19734350735
regulation of glycogen phosphorylase a phosphatase in neurospora crassa. 19734350266
selection of respiratory mutants of neurospora crassa.a method is described that permits the isolation of mutants that are defective in mitochondrial respiration. the techniques of inositol-less death and overlay with 2,3,5-triphenyl-2h-tetrazolium chloride are utilized to select for mutant colonies. colonies that survive inositol-less death and fail to reduce the tetrazolium dye are then tested polarographically for cyanide-sensitive respiration. a preliminary characterization of three mutants obtained by this method is presented. the mutants have ...19734349028
genetic analysis of purple adenine (ad-3) mutants induced by methyl methanesulfonate in neurospora crassa. 19734348927
behavior of cycloaldolase from neurospora crassa towards substrate analogs and aldolase inhibitors. 20154346519
ubiquinone function in neurospora crassa.mitochondria of cytoplasmic respiratory mutants [mi-1] (poky) and [mi-4] contain about a fourfold molar excess of ubiquinone as compared to the wild-type strain of neurospora crassa. in the wild type and [mi-1] cultures the concentration of ubiquinone remains constant during the exponential and stationary phase of growth. in [mi-4] cultures it markedly decreases in the stationary phase. the reduction of ubiquinone by substrates is approximately the same in the three strains tested and amounts 60 ...19724344917
different pool sizes of the precursor polypeptides of cytochrome oxidase from neurospora crassa. 19724344829
regulation of inositol biosynthesis in vivo by pyrophosphate in neurospora crassa. 19724344827
inhibition of the assembly of cytochrome oxidase in neurospora crassa by chloramphenicol. 19724344826
[polyphosphate depolymerase activity in cells of neurospora crassa (28610 as)]. 20164344204
effect of pyrrolnitrin on electron transport and oxidative phosphorylation in mitochondria isolated from neurospora crassa.pyrrolnitrin, at low concentrations, uncouples oxidative phosphorylation in neurospora mitochondria. at higher concentrations, pyrrolnitrin inhibits electron transport both in the flavine region and through cytochrome oxidase.19724343822
[localization and possible physiological role of polyphosphate depolymerases in neurospora crassa cells]. 19724343579
adenylate cyclase activity in neurospora crassa. i. general properties. 19724343160
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