Publications

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the specific interaction of chromate with the dual sulfate permease systems of neurospora crassa. 19715550165
isolation and chemical properties of a repressible acid phosphatase in neurospora crassa. 19715545084
a derepressible active transport system for glucose in neurospora crassa. 19715545073
metabolic role, regulation of synthesis, cellular localization, and genetic control of the glyoxylate cycle enzymes in neurospora crassa.the glyoxylate shunt enzymes, isocitrate lyase and malate synthase, were present at high levels in mycelium grown on acetate as sole source of carbon, compared with mycelium grown on sucrose medium. the glyoxylate shunt activities were also elevated in mycelium grown on glutamate or casamino acids as sole source of carbon, and in amino acid-requiring auxotrophic mutants grown in sucrose medium containing limiting amounts of their required amino acid. under conditions of enhanced catabolite repre ...19715541006
neurospora mutant deficient in tryptophanyl-transfer ribonucleic acid synthetase activity.a tryptophan auxotroph of neurospora crassa, trp-5, has been characterized as a mutant with a deficient tryptophanyl-transfer ribonucleic acid (trna) synthetase (ec 6.1.1.2) activity. when assayed by tryptophanyl-trna formation, extracts of the mutant have less than 5% of the wild-type specific activity. the adenosine triphosphate-pyrophosphate exchange activity is at about half the normal level. in the mutant derepressed levels of anthranilate synthetase and tryptophan synthetase were associate ...19715540999
chemical and physical studies of neurospora crassa invertase. molecular weight, amino acid and carbohydrate composition, and quaternary structure. 19715539234
variation of recombination frequency in neurospora crassa following temperature changes prior to and during meiosis and evidence for a premeiotic sensitive stage. 19705519984
[cell nuclei histones in neurospora crassa]. 19705519212
genetically mixed perithecia and somatic recombination in a pseudowild type strain of neurospora crassa. 19705516682
topography of the ad-3 region of neurospora crassa. 19705516678
a poly u directed phenylalanine polymerizing system from neurospora crassa: general characterization and ionic effects. 20135515872
[effect of 8-azaadenine on the metabolism of phosphorus compounds in neurospora crassa mutants auxotrophic with respect to adenine]. 19705515433
a suppressor of the heterokaryon-incompatibility associated with mating type in neurospora crassa. 19705512565
new crisp genes and crisp-modifiers in neurospora crassa. 19705512362
enzyme degradation of conidial wall during germination of neurospora crassa. 19705502332
purification and properties of homoserine dehydrogenase from neurospora crassa. 19705500942
mutants of neurospora crassa, escherichia coli and salmonella typhimurium specifically inhibited by carbon dioxide. 19705500023
analysis of a case of mutagen specificity in neurospora crassa. 3. fractionated treatment with diepoxybutane (deb). 19705497287
regulation of recombination at the his-3 locus in neurospora crassa. 19705496221
selection and characterisation of mutants lacking arginase in neurospora crassa. 19705495901
major constituents of the conidial wall of neurospora crassa. 19705494280
sugar transport in neurospora crassa. ii. a second glucose transport system. 19705492960
conserved mrna from the conidia of neurospora crassa. 19705491723
developmental regulation of amino acid transport in neurospora crassa.conidia of neurospora crassa exhibit an ability to transport various amino acids against a concentration gradient. the conidial transport system has previously been characterized in terms of kinetics, competitions, and genetic control. this study describes the development of a new and highly active transport capability which is elaborated during the early stages of development but prior to evident germination. it has been named "postconidial" transport activity and represents as much as 20-fold ...19705489433
a genetic analysis of non-disjunction and mitotic recombination in neurospora crassa. 19705488985
methods for the study of nuclear and cytoplasmic variation in respiratory activity of neurospora crassa, and the discovery of three new genes. 19705488472
analysis of a case of mutagen specificity in neurospora crassa. ii. interaction between treatments with diepoxybutane (deb) and ultraviolet light. 19705488083
inheritance of growth rate in neurospora crassa: crosses between previously selected lines. 19705487923
a nutritional method for the isolation of morphological mutants of neurospora crassa. 19705483778
genetic fine structure of the albino (al) region of neurospora crassa. 19705480750
genetic effects of n-methyl-n'-nitro-n-nitrosoguanidine in neurospora crassa. 19705480466
[electrophoretic analysis of carotenogenic proteins of neurospora crassa]. 19705478625
control of recombination within the nitrate-2 locus of neurospora crassa: an unlinked dominant gene which reduces prototroph yields. 19705477713
inhibition by d-glutamate of growth and glutamate dehydrogenase activity of neurospora crassa. 19705476895
nucleotide 'maps' of digests of deoxyribonucleic acid.various digests of (32)p-labelled dna were examined by two-dimensional ionophoresis on cellulose acetate and deae-cellulose paper. the products from digestion with pancreatic deoxyribonuclease and neurospora crassa endonuclease were qualitatively closely similar, but very complex, and were used to investigate the mapping behaviour of nucleotides in various ionophoretic systems. ionophoresis on deae-cellulose paper in triethylamine carbonate, ph 9.7, followed by ionophoresis in the second dimensi ...19705476726
tryptophan transport in neurospora crassa: a tryptophan-binding protein released by cold osmotic shock.osmotic shock treatment of germinated conidia of neurospora reduced the capacity for tryptophan transport in these cells approximately 90% without an appreciable loss of cell viability. tryptophan-binding proteins and alkaline phosphatase were consistently released into the osmotic shock fluid by this treatment. four lines of evidence suggest that the binding protein may be related to the tryptophan transport system. (i) it appears to be located on or near the cell surface. (ii) a decreased capa ...19705474881
feedback inhibition of l-glutamine d-fructose 6-phosphate amidotransferase by uridine diphosphate n-acetylglucosamine in neurospora crassa.the enzyme, l-glutamine d-fructose 6-phosphate amidotransferase (ec 2.6.1.16) of neurospora crassa, which catalyzes the formation of glucosamine 6-phosphate was shown to be subject to feedback inhibition by uridine diphosphate n-acetyl-d-glucosamine (udp-glcnac). the conclusion is based on the following observations. udp-glcnac, the direct precursor of chitin, did not accumulate in the cell even when its utilization for the synthesis of cell wall chitin was interrupted by the antibiotic polyoxin ...19705474877
histidine-mediated control of tryptophan biosynthetic enzymes in neurospora crassa.the formation of the five tryptophan biosynthetic enzymes of neurospora crassa was shown to be derepressed in histidine-starved cells. this histidine-mediated derepression was not due to a lowered intracellular concentration of tryptophan in these cells. furthermore, histidine-mediated derepression of tryptophan enzymes was found to be coordinate and not subject to reversal by tryptophan of either exogenous or biosynthetic origin. the synthesis of tryptophan enzymes also was found to be coordina ...19705473920
alterations of the respiratory system of neurospora crassa by the mi-1 mutation.the respiratory mechanism of the cytochrome-defective mi-1 mutant of neurospora crassa was partially characterized and compared with that of the wild type. both systems of electron transport were linked to oxidative phosphorylation and showed similar characteristics with respect to substrates and components. the system operating in the cytochrome-deficient mi-1 mutant showed no characteristic alpha absorption bands of cytochromes a or b and was very unstable in vitro. the possibility that this s ...19705473916
mechanism of action of the antifugal agent polyoxin d.the antibiotic polyoxin d was shown to inhibit the incorporation of (14)c-glucosamine into cell wall chitin in neurospora crassa at levels which were comparable with those required for inhibition of fungal growth. at the same time, the antibiotic increased the accumulation of a nucleotide, which was identified as uridine diphosphate (udp)-n-acetylglucosamine, indicating inhibition of chitin synthesis. chitin synthetase (udp-n-acetylglucosamine: chitin n-acetylglucosaminyl transferase, ec 2.4.1.1 ...19705473886
copurification of pyrimidine-specific carbamyl phosphate synthetase and aspartate transcarbamylase of neurospora crassa. 19705472708
the mechanism of uptake of cobalt ions by neurospora crassa.uptake of co(2+) by 3-day-old mycelia of neurospora crassa involves cell-surface binding as well as transport into the intracellular space. the surface binding is rapid and accounts for 30-40% of the total co(2+) uptake. transport of co(2+) occurs at a rate of 40mug/h per 100mg dry wt. surface binding and overall uptake show different temperature dependence. metabolic inhibitors such as azide, dinitrophenol and fluoride depress transport of co(2+). the overall uptake of co(2+) exhibits a high k( ...19705472176
an assessment of in vivo mitochondrial protein synthesis in neurospora crassa. 19705470823
[correlation of accumulation of certain fractions of inorganic polyphosphates and rna in neurospora crassa ad 28-610]. 19705470181
purification of neurospora crassa endonuclease-resistant dna-rna hybrid. 19705463194
amino acid transport in neurospora crassa. 3. acidic amino acid transport. 19705456980
[the effect of orotic acid hydrazide on the growth of neurospora crassa and on the development of ehrlich ascitic tumor]. 20135455884
nucleoside uptake during the germination of neurospora crassa conidia. 19705453517
genetic and biochemical studies of distinct sulfate permease species in different developmental stages of neurospora crassa. 19705446339
amino acid transport in neurospora crassa. ii. properties of a basic amino acid transport system. 19705445671
complications affecting the distinction between mendelian and maternally inheritd characters in neurospora crassa. 19705440053
sugar transport in neurospora crassa. 19705438359
isolation of neurospora crassa endonuclease specific for single-stranded dna. 19705438327
role of enzymes in growth and morphology of neurospora crassa: cell-wall-bound enzymes and their possible role in branching.the cell wall of neurospora crassa contains bound enzymes that can digest its structural polymers. these enzymes are not present at the same levels at all stages of growth. the levels of these autolytic enzymes vary and generally show some relationship to the process of branching. these enzymes were removed from the cell wall by beta-mercaptoethanol extraction and were tested for activity against isolated cell wall fractions. such studies, as well as autolytic studies, showed that enzymes acting ...19705438053
conversion of histidine to hercynine by neurospora crassa.cell-free extracts of neurospora crassa mycelium catalyze the methylation of l-histidine to form hercynine (histidine betaine). the partially methylated compounds, alpha-n-methyl-l-histidine and alpha-n,n-dimethyl-l-histidine, are also methylated by the same enzyme preparation to form hercynine. all three methylation reactions are stimulated by the addition of s-adenosylmethionine.19705438052
[effect of antimetabolites on a neurospora crassa mutant resistant to 5-azarotic acid]. 19705433931
the beta-galactosidase system of neurospora crassa. ii. extracellular nature of the ph 4.2 enzyme. 19705433578
the beta-galactosidase system of neurospora crassa. i. purification and properties of the ph 4.2 enzyme. 19705433577
pyrimidine-specific carbamyl phosphate synthetase in neurospora crassa.two carbamyl phosphate synthetases, the first an arginine-synthetic enzyme (cps(arg)) and the second a pyrimidine-synthetic enzyme (cps(pyr)), are shown to be present in neurospora. the two enzymes can be separated on the basis of size and are distinguished by several different properties. both cps(pyr) and cps(arg) have substrate requirements of adenosine triphosphate, hco(3) (-), and l-glutamine, although nh(4) (+) in high concentration will partially replace glutamine. cps(pyr) activity can b ...19705432004
genetic and metabolic controls for sulfate metabolism in neurospora crassa: isolation and study of chromate-resistant and sulfate transport-negative mutants.mutants of neurospora resistant to chromate were selected and all were found to map at a single genetic locus designated as cys-13. the chromate-resistant mutants grow at a wild-type rate on minimal media but are partially deficient in the transport of inorganic sulfate, especially during the conidial stage. an unlinked mutant, cys-14, is sensitive to chromate but transports sulfate during the mycelial stage at only 25% of the wild-type rate; cys-14 also grows at a fully wild-type rate on minima ...19705429722
[enzyme patterns during conidiogenesis in neurospora crassa]. 19705429635
mutagenicity of aflatoxins in neurospora crassa. 19705424724
mutagenic action of nitrous acid on two transversions in the histidine-3 locus of neurospora crassa. 19705424718
effect of cycloheximide on the synthesis and modification of ribosomal rna in neurospora crassa. 19705421964
the gemini-xi s-4 spaceflight-radiation interaction experiment. 3. comparison of the spectra of recessive lethal mutations at specific loci in the ad-3 region in neurospora crassa. 19705421854
arginaseless neurospora: genetics, physiology, and polyamine synthesis.four arginaseless mutants of neurospora crassa have been isolated. all carry mutations which lie at a single locus, aga, on linkage group viir. a study of aga strains shows the arginase reaction to be the major, perhaps the only, route of arginine consumption in neurospora other than protein synthesis. ornithine-delta-transaminase, the second enzyme of the arginine catabolic pathway, is present and normally inducible by arginine in aga strains, and ornithine transcarbamylase, an enzyme of argini ...19705419257
isoenzymes and allosteric inhibition of neurospora crassa 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase. 19705416121
[selection of actidion-resistant mutants in neurospora crassa and their genetical and biochemical analysis]. 19705415255
localization of the beta-glucosidases in neurospora crassa.the beta-glucosidases (ec 3.2.1.21) of neurospora crassa were studied with respect to their location in conidia and young mycelia. aryl-beta-glucosidase of conidia was nearly equally divided between extracellular and bound activity. bound aryl-beta-glucosidase was almost all available to substrate. an induction procedure was used to maximize both beta-glucosidases in 4 to 6-hr cells. aryl-beta-glucosidase was entirely bound but still mostly (90%) detectable, whereas cellobiase was mostly interna ...19705413819
characterization of icr-177-induced ad-3 mutants of neurospora crassa. 19705413676
in vivo and in vitro complementation between dhq synthetase mutants in the arom gene cluster of neurospora crassa. 19695399249
a sequence of colonial mutants with practical application for mutation studies in neurospora crassa. 19695399227
neurospora crassa glycogen phosphorylase: characterization and kinetics via a new radiochemical assay for phosphorolysis. 19695391473
amino acid induction of conidiation and morphological alterations in wild type and morphological mutants of neurospora crassa. 19695384626
genetic control of phenylalanine and tyrosine biosynthesis in neurospora crassa. 19695384488
the iv-3 mutants of neurospora crassa. ii. activity of acetohydroxy acid synthetase. 19695384481
the iv-3 mutants of neurospora crassa. i. genetic and biochemical characteristics. 19695384480
electrophoretic comparison of cytoplasmic and mitochondrial ribosomal proteins from neurospora crassa. 20135380348
control of trehalase synthesis in neurospora crassa. 20135373655
the relation between growth, conidiation and trehalase activity in neurospora crassa. 20095373654
[comparative study of the alanine-glyoxylate transaminase and malate synthetase in neurospora crassa]. 19695372863
treatment of three-point linkage data in neurospora crassa. 19695371748
preparation of isolated macroconidia of neurospora crassa. 19695371447
electron-dense structures in neurospora crassa. ii. lomasome-like structures. 19695370983
electron-dense structures in neurospora crassa. i. mesosomes. 19695370982
genetic control of flanking marker behaviour in an allelic cross of neurospora crassa. 19695370979
regulation of amino acid assimilation in a strain of neurospora crassa lacking basic amino acid transport activity. 19695368248
new markers and map sequences in neurospora crassa, with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. 19695367562
ribosomal changes related to changes in the rate of growth in neurospora crassa mycelia. 19695366938
the biosynthesis of cytidine diphosphate esters in rat liver and neurospora crassa. 19695366838
reversion of acridine mustard-induced ad-3 mutants of neurospora crassa. 20005366005
the radiation sensitivity of nuclease mutants in neurospora crassa. 20005366004
isolation and characterization of nuclease mutants in neurospora crassa. 19695365296
mutants in the arom gene cluster of neurospora crassa specific for biosynthetic dehydroquinase. 19695364964
interconvertible forms of glycogen synthetase in neurospora crassa. 19695363997
pyrimidine synthesis in neurospora crassa: regulation of enzyme activities.the regulation of several enzymes involved in pyrimidine biosynthesis in neurospora crassa has been studied. elevation of atcase (l-aspartate carbamoyltransferase) activity is found in all pyrimidine-requiring mutants when they are starved for uridine. dhoase (dihydroorotase) is an unstable enzyme, and it is impossible to conclude what type of regulation, if any, controls this enzyme. dhodehase (dihydroorotate dehydrogenase) activity shows a marked elevation in uridine-starved pyr-2 cultures, a ...19695361219
pyrimidine synthesis in neurospora crassa: gene-enzyme relationships.a new series of pyrimidine-requiring mutants of neurospora has been isolated and all enzymes involved in pyrimidine biosynthesis are represented by at least one mutant. among these mutants is included a single isolate for a new locus, pyr-6. this mutant is deficient in dihydroorotase (dhoase) and represents the only enzymatic step in orotate synthesis for which no mutant previously had been found. this mutant, which mapped genetically on the right arm of linkage group v, is unlinked to any of th ...19695361218
[immunochemistry and electrophoresis of the conidiation of neurospora crassa]. 19695357827
some aspects of complementation with carotenogrenic al loci in neurospora crassa. 19695357122
incorporation in vivo of 14c-labelled amino acids into the proteins of mitochondrial ribosomes from neurospora crassa sensitive to cycloheximide and insensitive to chloramphenicol. 19695356628
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