study on immobilization of aminoacylase from aspergillus oryzae on macroporous copolymers.the aminoacylase from aspergillus oryzae was adsorbed on polymer carriers where the enzyme showed excellent catalyzing activity and operating stability. it was found that the pore structure and functional group were the basic factors which affected the activity of the adsorbed enzyme. the hydrophobility of the carriers did not play an important role in the enzymatic resolution. various factors, such as percentage and nature of cross-linking agent, porogenic agents and functionalizing agents whic ...19873452428
transformation of aspergillus oryzae using the a. niger pyrg gene.a transformation system for aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrg) was developed. transformation frequencies of up to 16 transformants per microgram of dna were obtained with the vector pab4-1, which carries the pyrg gene of a. niger. southern blotting analysis showed that vector dna sequences were integrated into the chromosomal dna, in various copy numbers and presumably at different sites. efficient cotransformation of an unselectable gene was also sh ...19873481025
new alpha-amylase and trypsin inhibitors among the cm-proteins of barley (hordeum vulgare).barley cm-proteins are a group of at least five salt-soluble components (cma-e) that can be selectively extracted from endosperm with chloroform/methanol mixtures. n-terminal sequences of proteins cma, cmb and cmc have been determined and found to be homologous to those previously determined for cmd and cme, an observation which confirms that their structural genes are members of a dispersed multi-gene family. the purified cm-proteins were tested against trypsin and against alpha-amylases from s ...19863484638
nucleotide sequence of the alpha-amylase gene (alp1) in the yeast saccharomycopsis fibuligera.the complete nucleotide sequence of the secretable alpha-amylase gene alp1 from the yeast saccharomycopsis fibuligera has been determined. the alp1 dna hybridized to a polyadenylated rna of 2.0 kilobases. a single open reading frame encodes a 494-amino acid protein which is highly homologous with alpha-amylase (taka-amylase) of a fungus aspergillus oryzae.19873497057
differential scanning calorimetric study of the thermal unfolding of taka-amylase a from aspergillus oryzae.the thermally induced unfolding of taka-amylase a, isolated from aspergillus oryzae, was studied by differential scanning calorimetry. the experimental curves of excess apparent specific heat vs. temperature showed a single asymmetric peak. curve resolution indicated that this asymmetry is due to the two-state unfolding of three domains in the molecule, with dissociation of the single tightly bound ca2+ ion occurring during the unfolding of the last domain. further indication of the dissociation ...19873498514
studies on the active site of taka-amylase a: its action on phenyl maltooligosides with a charge at their non-reducing-ends.five modified moltooligosaccharides, phenyl o-6-amino-6-deoxy-alpha-d- glucopyranosyl- (1----4)-o-alpha-d-glucopyranosyl-(1----4)-o-alpha-d-glucopyranosyl-(1-- --4)- alpha-d-glucopyransoide (ag4p), phenyl o-(alpha-d-glucopyranosyluronic acid)-(1----4)-o-alpha-d-glucopyranosyl-(1----4)-o-alpha-d-glucopyran osy l- (1----4)-alpha-d-glucopyranoside (cg4p), phenyl o-6-amino-6-deoxy-alpha-d-glucopyranosyl-(1----4)-o-alpha-d-glucopyra nos yl- (1----4)-o-alpha-d-glucopyranosyl-(1----4)-o-alpha-d-glucopy ...19873501785
effect of yeast culture and aspergillus oryzae fermentation extract on ruminal characteristics and nutrient digestibility.four nonpregnant and nonlactating holstein cows fitted with ruminal fistulas were assigned to each of four diets in a 4 x 4 latin square design. dietary treatments were 1) basal diet containing 50% concentrate; 2) basal diet plus 90 g/d yeast culture; 3) basal diet plus 2.63 g/d aspergillus oryzae fermentation extract; 4) basal diet plus 90 g/d of a. oryzae fermentation extract and yeast culture. cows were fed diets at a rate of 86 g dm/kg bw.75 for 14 d adaptation followed by an 8-d collection ...19873680724
improved purification of aspergillus oryzae 1,2-alpha-mannosidase by using mannan gel affinity order to facilitate the purification of 1,2-alpha-mannosidase from an enzyme product of aspergillus oryzae, we have devised a rapid and simple procedure. a partially purified enzyme preparation obtained from the a. oryzae enzyme product, by means of ammonium sulfate fractionation followed by cm-sephadex c-50 chromatography, was subjected to affinity chromatography with baker's yeast mannan gel as an adsorbent. 1,2-alpha-mannosidase was retarded and well separated from the major protein peak o ...19863700367
the disulphide folding pathway of ribonuclease t1.the pathway of unfolding and refolding that accompanies, respectively, the breakage and formation of the two disulphide bonds of ribonuclease t1 has been determined kinetically and compared with those of other proteins.19863735429
invasion of paranasal sinuses by aspergillus oryzae.a 24-year-old male patient receiving chemotherapy for acute promyelocytic leukemia developed fever, right periorbital swelling and mild right proptosis. a head scan showed opacification of the right maxillary and ethmoid sinuses with adjacent soft tissue swelling. biopsy of the nasal mucosa demonstrated the typical septate hyphae of aspergillus species which was later shown on culture to be aspergillus oryzae. a. oryzae has only rarely been reported in human disease and there is confusion as to ...19863796709
[interrelation of translation regulation and amino acid uptake into cells]. 19853877528
[protease and alpha-amylase synthesis by washed cells of aspergillus oryzae 251-90].regularities of protease and alpha-amylase production by washed cells of aspergillus oryzae 251-90 were being studied. the results obtained enabled us to assume a constitutive character of the both enzymes synthesis by the given producer. sources of carbon, nitrogen and sulphur take part in regulation of the protease production, whereas in the case of the alpha-amylase synthesis only carbon sources that are important. elimination of phosphorus from the medium affects the synthesis of both enzyme ...19853885211
nucleotide sequence of the extracellular glucoamylase gene sta1 in the yeast saccharomyces diastaticus.the complete nucleotide sequence of the extracellular glucoamylase gene sta1 from the yeast saccharomyces diastaticus has been determined. a single open reading frame codes for a 778-amino-acid protein which contains 13 potential n-glycosylation sites. in the 5'- and 3'-flanking regions of the gene, there are striking sequence homologies to the corresponding regions of adh1 for alcohol dehydrogenase and mat alpha 2 for mating type control in the yeast saccharomyces cerevisiae. the putative precu ...19853918017
enzyme replacement with liposomes containing beta-galactosidase from charonia lumpas in murine globoid cell leukodystrophy (twitcher).enzyme replacement with liposomes containing beta-galactosidase obtained from charonia lumpas was carried out in murine globoid cell leukodystrophy (gld). charonia lumpas beta-galactosidase was able to hydrolyze galactocerebroside trapped into liposomes prepared from lecithin, cholesterol and sulfatide (molar ratio; 7:2:1). liposomes containing charonia lumpas beta-galactosidase were successfully incorporated into the mouse tissues. 3h-galactocerebroside labeled liposomes were also incorporated ...19853919736
[mycoses of the orbit and the eye]. 19853922126
effect of additive on alfalfa silage fermentation characteristics and feedlot performance of each of two trials four large plastic bags were filled with approximately 72 mt of first cutting, early bloom alfalfa (35% dry matter). fresh alfalfa in two bags was treated with .5 kg/metric ton of a commercial silage additive (silo-best). two bags were left untreated. when compared with steers fed untreated alfalfa silage, weight gains, intakes, and feed efficiencies were not significantly (p greater than .05) altered for steers fed treated silage. however, laboratory analysis showed silage ...19853928719
purification and characterization of endo-beta-n-acetylglucosaminidase of aspergillus endo-beta-n-acetylglucosaminidase which hydrolyzes the n,n'-diacetylchitobiosyl linkage in asparagine-linked oligosaccharides was purified from the enzyme product of aspergillus oryzae. its substrate specificity was similar to that of endo-beta-n-acetylglucosaminidase h from streptomyces griseus with respect to the relative activities toward the glycopeptides obtained from ovalbumin and bovine igg. the present endoglycosidase exhibited a broad optimum ph range and was relatively stable. metal ...19853934149
evidence that the mechanisms of fungitoxicity of 8-quinolinol and its bischelate with copper(ii) are different.the copper(ii) contents of the growth media, sabouraud dextrose and czapek-dox broths, and of the spore inocula of aspergillus niger (atcc 1004), aspergillus oryzae (atcc 1011), trichoderma viride (atcc 8678), and myrothecium verrucaria (atcc 9095) were determined by atomic absorption spectrophotometry in a graphite furnace. the test systems composed of sabouraud dextrose broth and spore inocula of the four fungi contained only a little over 3% of the copper(ii) required to form a minimal inhibi ...19853935305
the subsite structures of guanine-specific ribonucleases and a guanine-preferential ribonuclease. cleavage of oligoinosinic acids and poly order to estimate the size of the active site of guanylic acid specific rnases (rnase t1 from aspergillus oryzae and rnase st from streptomyces erythreus) and guanine-preferential rnase (rnase ms from a. saitoi), the depolymerization reaction of oligoinosinic acid, (ip)ni greater than p, having various chain lengths was studied. the kinetic parameters for depolymerization of oligoinosinic acids, (pkm, log v and log v/km) by the three rnases increased with increase of the chain length of the s ...19853936847
a novel enzyme producing isoprimeverose from oligoxyloglucans of aspergillus enzyme producing isoprimeverose from xyloglucan fragment oligosaccharides has been purified to the electrophoretically pure state from a commercial enzyme preparation of aspergillus oryzae (sanzyme 1000). the purified enzyme showed approximately 1,280-fold increase of the specific activity over the original preparation. the purified enzyme was shown to be an oligomeric protein consisting of two subunits, each of which had a molecular weight of 115,000. the enzyme showed the highest activity a ...19854019436
antifungal properties of 3-n-alkyn-1-ols and synergism with 2-n-alkyn-1-ols and ketoconazole.twelve 3-n-alkyn-1-ols (c4-c12, c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by ph of the medium, and significantly ...19854020633
heterokaryosis between aspergillus oryzae cyclopiazonic acid-defective strains: method for estimating the risk of inducing toxin production among cyclopiazonic acid-defective industrial strains.aspergillus oryzae strains are used extensively in the food industry. some of these strains excrete alpha-cyclopiazonic acid (cpa), a mycotoxin which may provoke toxicoses in rats. physicochemical methods may reveal the presence of this toxin, but they are inadequate to screen cpa-nonproducing (cpa-) strains. cpa production is revealed by either bacterial growth inhibition or alkalinization of the culture medium. this first biological property was used to devise a time-saving screening method to ...19854083874
properties of proteases from aspergillus oryzae and aspergillus flavus. a comparative study. 19714108471
specificity of the dna product of rna-dependent dna polymerase in type c viruses. ii. quantitative analysis.a number of mammalian type c viruses were analyzed for relatedness by the technique of dna.rna hybridization. viral dnas were prepared in single-stranded form from complexes with 70s viral rna formed during endogenous polymerase reactions. extent of hybridization was assayed with the single-strand nuclease (s-1) from aspergillus oryzae. results obtained indicated a high degree of viral specificity, with significant cross-reactions being observed only with viruses obtained from within a species, ...19734129928
on the pharmacology of brinases, a proteolytic enzyme from aspergillus oryzae. 19744134257
clinical review on brinase, a protease from aspergillus oryzae. 19744134258
review of the biochemistry and coagulation physiology of brinolase. (fibrinolytic enzyme from aspergillus oryzae). 19744134481
[experimental and clinical studies on a protease from aspergillus oryzae (brinase): fibrinogen turnover, thrombocyte aggregation and occlusion of the scribner shunt]. 19744134482
dynamics of amylolytic enzymes synthesis in aspergillus oryzae. 19664161862
the purification and properties of an extracellular protease from aspergillus oryzae nrrl 2160. 19734197025
alpha-d-fucosidase from aspergillus oryzae: characterization of alpha-d-fucosidase with alpha-d-galactosidase activity. 19734198930
[demonstration of extracellular leucine aminopeptidases (ec 3.4.1) of aspergillus oryzae (ip 410). study of the leucine aminopeptidase 2 fraction]. 19744212604
[products of proteolysis obtained with the use of complex enzyme preparations at different ph values]. 19744219043
[extracellular leucine aminopeptidases (ec of aspergillus oryzae (ip 410). glycoprotein nature of the fraction leucine aminopeptidase 1]. 19744219346
in vitro studies on a proteolytic enzyme from aspergillus oryzae (protease i). 19684231457
on the inhibition of a fibrinolytic enzyme from aspergillus oryzae by serum. 19694243348
influence of an enzyme from aspergillus oryzae, protease i, on some components of the fibrinolytic system. 19704246604
a nuclease specific for heat-denatured dna in isolated from a product of aspergillus oryzae. 19664287053
on the deoxyribonucleases, k1 and k2, isolated from mycelia of aspergillus oryzae. i. isolation and purification of dnases k1 and k2. 19684303413
the two deoxyribonucleases, k1 and k2, isolated from mycelia of aspergillus oryzae. ii. determination of 5'- and 3'-termini of the dna fragments produced by dnases k1 and k2. 19684303414
studies on the respiratory system of aspergillus oryzae. 3. properties of mitochondria from mycelia grown in the presence of antimycin a. 19714322618
studies on the respiratory system of aspergillus oryzae. iv. low temperature difference spectra of mitochondria from mycelia grown in the presence and absence of chloramphenicol. 19724335785
studies on n-acetyl- -d-glucosaminidase of aspergillus oryzae. 3. transglycosylation by the enzyme and preparation of crystalline phenyl n, n'-diacetyl- -chitobioside. 19724346265
factors affecting the synthesis and release of the extracellular protease of aspergillus oryzae nrrl 2160. 19734351078
action of the s1 endonuclease from aspergillus oryzae on simian virus 40 supercoiled component i dna. 19734356815
cleavage of circular, superhelical simian virus 40 dna to a linear duplex by s1 nuclease.s(1) nuclease, the single-strand specific nuclease from aspergillus oryzae can cleave both strands of circular covalently closed, superhelical simian virus 40 (sv40) dna to generate unit length linear duplex molecules with intact single strands. but circular, covalently closed, nonsuperhelical dna, as well as linear duplex molecules, are relatively resistant to attack by the enzyme. these findings indicate that unpaired or weakly hydrogen-bonded regions, sensitive to the single strand-specific n ...19734357509
studies on a pyrophosphatase and glucose-6-phosphatase from aspergillus oryzae. 19734358816
partial transcription of murine type c viral genomes in balb c cell lines.the mouse cell line, balb/c 3t3, and its derivatives transformed either spontaneously or by treatment with a variety of external agents, were analyzed for cytoplasmic rna complementary to dna products prepared from the kirsten strain of murine sarcoma-leukemia virus, and from an endogenous type c virus of balb/c 3t3. although none of these cell lines spontaneously releases complete type c virions, they all contain rna which is partially homologous to a portion of the 35s rna isolated from these ...19734359949
the formation, general characteristics and production of alpha-amylase in heterocaryons and diploids of aspergillus oryzae. 19744436159
amino acid transport in spores of aspergillus oryzae. 19744436276
[isolation of fractions having aminopeptidase activity (ec (arylamidase) from culture medium of aspergillus oryzae]. 19744447798
[the effect of sodium chloride and sulfate on the growth and amylolytic activity of the fungus aspergillus oryzae]. 19744449783
[biosynthesis of beta-fructofuranosidase by fungi of the genus aspergillus]. 19744463361
[free intracellular amino acids of aspergillus oryzae mycelia and acid proteinase synthesis]. 19744464877
microbial formation of nitrosamines in vitro.mortierella parvispora and an unidentified bacterium converted trimethylamine to dimethylamine, and the bacterium (but not the fungus) formed dimethylnitrosamine in the presence of nitrite. dimethylnitrosamine also appeared in cell suspensions of escherichia coli and streptococcus epidermidis and in hyphal mats of aspergillus oryzae incubated with dimethylamine and nitrate. suspensions of a number of microorganisms produced n-nitrosodiphenylamine from diphenylamine and nitrite at ph 7.5, and sol ...19734577486
[effect of the chemical structure of polymers on the activity of certain enzymes synthesized by cultures of aspergillus oryzae and bacillus mesentericus]. 19734600723
induction of enzymes in dormant spores of aspergillus oryzae. 3. effects of kanamycin and streptomycin. 19724639285
induced morphogenetic variations in aspergillus oryzae by 8-azaguanine. 19724654227
studies on n-acetyl-beta-d-glucosaminidase of aspergillus oryzae. iv. acceptor specifity and quantitative representation of the transglycosylation reaction. 19724664739
[mechanism of the effect of starch on the biosynthesis of alpha-amylase by aspergillus oryzae]. 19724670464
purification and properties of an isoenzyme of arylsulphatase from aspergillus oryzae. 19734685274
studies on n-acetyl- -d-glucosaminidase of aspergillus oryzae. v. demonstration of an exo-type of the enzyme and determination of partial binding energy of the es-complex. 19734690234
purification and further properties of single-strand-specific nuclease from aspergillus oryzae. 19734691350
[effect of exogenous urea on the amylolytic and proteolytic activities of the fungus aspergillus oryzae]. 19734701626
[localization of the effect of regulation of alpha-amylase biosynthesis by the level of active enzyme in the medium with aspergillus oryzae]. 19734704556
induction of enzymes in dormant spores of aspergillus oryzae. iv. effects of ethidium, aminoacridines, and related compounds. 19734708042
use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes.bacterial and plasmid homo- and heteroduplexes have been analyzed with a single-strand specific endonuclease, s1, of aspergillus oryzae. under appropriate assay conditions, there was a high degree of correlation between the degree of deoxyribonucleic acid (dna)-dna homoduplex formation assessed by the s1 endonuclease and by hydroxyapatite (ha). heteroduplexes which contain extensive regions of polynucleotide sequences in common are similarly recognized by the s1 endonuclease and ha. in instances ...19734728274
stimulation of induction of glucose dehydrogenase by homologous series of aliphatic alcohols in dormant spores of aspergillus oryzae. 19734729444
isolation of alkaline proteinases from aspergillus oryzae by one-step affinity chromatography on ovoinhibitor-sepharose column. 19734736151
self-inhibiting extracellular proteins from aspergillus oryzae. 19734744881
growth and fat formation of aspergillus oryzae and aspergillus terreus on enzyme-hydrolyzed sweet potatoes. 19734763732
[comparative hygienic evaluation of the complex enzyme preparations obtained from the fungus aspergillus oryzae]. 19734793429
fiber digestion in lambs fed an extract of aspergillus oryzae. 19734797042
[selection of producers of amylolytic enzymes]. 19734805933
further evidence on differentiation of aspergillus sojae from aspergillus oryzae by electrophoretic patterns of cellulase, pectin-lyase, and acid proteinase. 19744822056
study of a microbial aminopeptidase from aspergillus oryzae determination of the n-terminal sequences of native proteins and of peptides. 19694887491
leucyl-pepsin a product of hog pepsinogen activation by proteinases of aspergillus oryzae. 19694900139
immunochemical studies on alpha-amylase. i. effects of denaturing agents and proteolytic enzymes on the immunochemical reactivity of alpha-amylase from aspergillus oryzae. 19654955963
immunochemical studies on alpha-amylase. ii. examination of immunochemical and enzymic activities of native and modified alpha-amylase from aspergillus oryzae. 19654955964
studies on the glucose dehydrogenase of aspergillus oryzae. i. induction of its synthesis by rho-benzoquinone and hydroquinone. 19674962256
on the two deoxyribonucleases, k1 and k2, isolated from mycelia of aspergillus oryzae. v. piece-meal hydrolysis of native dna effected by dnase k2. 19694977164
[biosynthesis of glycoproteins. xvii. characterization of a mannosyltransferase from aspergillus oryzae]. 19714996438
purification and properties of an arylsulfatase from aspergillus oryzae. 19714998894
studies on the conidia of aspergillus oryzae. xi. latent ribonuclease in the conidia of aspergillus oryzae. 19715001286
studies on n-acetyl- -d-glucosaminidase of aspergillus oryzae. ii. substrate specificity of the enzyme. 19725015362
alkaline protease from aspergillus oryzae: esterase activity. 19725018277
[role of the cell membrane in regulating the biosynthesis of alpha-amylase in aspergillus oryzae]. 19725037362
differentiation of aspergillus sojae from aspergillus oryzae by polyacrylamide gel disc electrophoresis. 19725039255
thrombolytic properties and side effects of brinase (fibrinolytic enzyme from aspergillus oryzae) in the dog. 19725070267
the effects of brinolase (fibrinolytic enzyme from aspergillus oryzae) on platelet aggregation of dog and man. 19725074991
purification and properties of a new alpha-glucanase from aspergillus oryzae. 19715089323
minimum number of proteases occurring in culture media of aspergillus oryzae. 19715098345
conditions and course of accumulation and excretion of amylases in cultures of aspergillus oryzae. 19715112095
reaction of protein disulfide groups with ellman's reagent: a case study of the number of sulfhydryl and disulfide groups in aspergillus oryzae -amylase, papain, and lysozyme. 19715114933
purification of ribonuclease t 1 by diethylaminoethylcellulose chromatography.a procedure is described for isolating the enzyme ribonuclease t(1) from takadiastase, an extract of the mould aspergillus oryzae. it involves an initial concentration of the enzyme by adsorption on deae-cellulose followed by gradient elution. later the enzyme is chromatographed on the same adsorbent with an eluent of constant composition. yields of 350-380mg of ribonuclease t(1) from 500g of takadiastase were obtained.19715114970
[study of the structure of the enzyme complex from a culture of aspergillus oryzae 8f-1 by electrophoresis in polyacrylamide gel and immunoelectrophoresis]. 19715129018
synthesis of l-3,4-dihydroxyphenylalanine from l-tyrosine by microorganisms.l-3, 4-dihydroxyphenylalanine can be synthesized efficiently from l-tyrosine under acidic conditions by aspergillus oryzae and other fungi.19715130435
complement dependent cytotoxic autoantibodies in patients with acute leukaemia following fibrinolytic therapy by protease 1 of aspergillus oryzae. 19705268985
[on the microbiological conversion of n-containing substrates. 4. on the microbiological conversion of 5-hydroxyindole through clavic eps purpurea, cordyceps militaris and aspergillus oryzae]. 19685302347
oxidative phosphorylation and respiratory control in mitochondria from aspergillus oryzae. 19695344753
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