improvement of omega-3 docosahexaenoic acid production by marine dinoflagellate crypthecodinium cohnii using rapeseed meal hydrolysate and waste molasses as feedstock.rapeseed meal and waste molasses are two important agro-industrial by-products which are produced in large quantities. in this study, solid state fermentation and fungal autolysis were performed to produce rapeseed meal hydrolysate (rmh) using fungal strains of aspergillus oryzae, penicillium oxalicum and neurospora crassa. the hydrolysate was used as fermentation feedstock for heterotrophic growth of microalga crypthecodinium cohnii that produce docosahexaenoic acid (dha). the addition of waste ...201525942565
purification and enzymatic characterization of secretory glycoside hydrolase family 3 (gh3) aryl β-glucosidases screened from aspergillus oryzae a global search of the genome database of aspergillus oryzae, we found 23 genes encoding putative β-glucosidases, among which 10 genes with a signal peptide belonging to glycoside hydrolase family 3 (gh3) were overexpressed in a. oryzae using the improved glaa gene promoter. consequently, crude enzyme preparations from three strains, each harboring the genes ao090038000223 (bgla), ao090103000127 (bglf), and ao090003001511 (bglj), showed a substrate preference toward p-nitrophenyl-β-d-glucopyr ...201525936960
repeated-batch operation for the synthesis of lactulose with β-galactosidase immobilized by aggregation and crosslinking.synthesis of lactulose under repeated-batch operation was done with cross-linked aggregates of aspergillus oryzae β-galactosidase (clags). the effect of the crosslinking agent to enzyme mass ratio and cross-linking time were first evaluated. best results were obtained at 5.5gdeglutaraldehyde/g enzyme at 5h of cross-linking, obtaining a specific activity of 15,000iug(-1), with 30% immobilization yield. clag was more stable than the free enzyme under non-reactive conditions with a half-life of 123 ...201525935392
a fungal biofilm reactor based on metal structured packing improves the quality of a gla::gfp fusion protein produced by aspergillus oryzae.fungal biofilm is known to promote the excretion of secondary metabolites in accordance with solid-state-related physiological mechanisms. this work is based on the comparative analysis of classical submerged fermentation with a fungal biofilm reactor for the production of a gla::green fluorescent protein (gfp) fusion protein by aspergillus oryzae. the biofilm reactor comprises a metal structured packing allowing the attachment of the fungal biomass. since the production of the target protein is ...201525935344
effect of adding cofactors to exogenous fibrolytic enzymes on preingestive hydrolysis, in vitro digestibility, and fermentation of bermudagrass haylage.our objectives were to examine if adding metal ion cofactors (cof) to exogenous fibrolytic enzymes (efe) would increase the beneficial effects of the efe on the preingestive hydrolysis and in vitro digestibility and fermentation of bermudagrass haylage. in experiment 1, 5 cof (mn(2+), co(2+), fe(2+), ca(2+), and mg(2+)) were screened to select the best candidates for synergistically enhancing release of water-soluble carbohydrates (wsc) from bermudagrass haylage by 5 efe. the 5 efe (1a, 2a, 11c, ...201525935239
molecular characterization of a penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins.we previously described an endo-acting rhamnogalacturonan (rg) lyase, termed pcrgl4a, of penicillium chrysogenum 31b. here, we describe a second rg lyase, called pcrglx. we determined the cdna sequence of the pcrglx gene, which encodes pcrglx. based on analyses using a blast search and a conserved domain search, pcrglx was found to be structurally distinct from known rg lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of nectria haematoco ...201525921806
hybrid de novo genome assembly using miseq and solid short read data.a hybrid de novo assembly pipeline was constructed to utilize both miseq and solid short read data in combination in the assembly. the short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such as abyss and soapdenovo. the assembly pipeline proceeded through several stages, and either miseq paired-end data, solid mate-paired data, or both of them could be specified as input data at each stage separately. the pipeline was examined on the ...201525919614
signal peptide optimization tool for the secretion of recombinant protein from saccharomyces cerevisiae.the secretion efficiency of foreign proteins in recombinant microbes is strongly dependent on the combination of the signal peptides (sps) used and the target proteins; therefore, identifying the optimal sp sequence for each target protein is a crucial step in maximizing the efficiency of protein secretion in both prokaryotes and eukaryotes. in this study, we developed a novel method, named the sp optimization tool (spot), for the generation and rapid screening of a library of sp-target gene fus ...201525912446
galacto configured n-aminoaziridines: a new type of irreversible inhibitor of β-galactosidases.a new type of galactose mimetics has been synthesized following a straightforward synthetic approach based on cyclohexene olefin aziridination reactions directed by hydroxyl substituents. these enantiomerically pure galacto-configured n-aminoaziridines are potent irreversible inhibitors of aspergillus oryzae and escherichia coliβ-galactosidases.201525895752
survey of protein-dna interactions in aspergillus oryzae on a genomic scale.the genome-scale delineation of in vivo protein-dna interactions is key to understanding genome function. only ∼5% of transcription factors (tfs) in the aspergillus genus have been identified using traditional methods. although the aspergillus oryzae genome contains >600 tfs, knowledge of the in vivo genome-wide tf-binding sites (tfbss) in aspergilli remains limited because of the lack of high-quality antibodies. we investigated the landscape of in vivo protein-dna interactions across the a. ory ...201525883143
γ-aminobutyric acid (gaba) production and angiotensin-i converting enzyme (ace) inhibitory activity of fermented soybean containing sea tangle by the co-culture of lactobacillus brevis with aspergillus enhance the γ-aminobutyric acid (gaba) content, the optimized fermentation of soybean with added sea tangle extract was evaluated at 30°c and ph 5.0. the medium was first inoculated with aspergillus oryzae strain fmb s46471 and fermented for 3 days, followed by the subsequent inoculation with lactobacillus brevis gaba 100. after fermentation for 7 days, the fermented soybean showed approximately 1.9 g/kg gaba and exhibited higher ace inhibitory activity than the traditional soybean product. f ...201525876604
anti-oxidant and anti-adipogenic effects of ethanol extracts from wheat germ and wheat germ fermented with aspergillus oryzae.most of the wheat germ in cereal grains is removed during the milling process. various physiological effects have been reported for bioactive substances in wheat germ such as phenolic acids and flavonoids. in this study, the anti-oxidant and anti-adipogenic effects of ethanol extracts from wheat germ (wge) and wheat germ fermented with aspergillus oryzae (f-wge) were investigated in hepg2 and 3t3-l1 cells. the anti-oxidant activity of f-wge was demonstrated by a dose-dependent increase in the en ...201525866747
reconstitution of biosynthetic machinery for the synthesis of the highly elaborated indole diterpene penitrem.penitrem a is one of the most elaborated members of the fungal indole diterpenes. two separate penitrem gene clusters were identified using genomic and rna sequencing data, and 13 out of 17 transformations in the penitrem biosynthesis were elucidated by heterologous reconstitution of the relevant genes. these reactions involve 1) a prenylation-initiated cationic cyclization to install the bicyclo[3.2.0]heptane skeleton (ptme), 2) a two-step p450-catalyzed oxidative processes forming the unique t ...201525831977
study of the mechanisms of a japanese traditional fermented medicine in the improvement of constipation.gut working tablet (gwt) is a japanese traditional fermented medicine based on aspergillus oryzae nk-fermented grain germ. although gwt has been used by patients with constipation, the mechanism has not been investigated. the aim of this study was to examine the possible mechanisms of the effect of gwt on constipation.201525827805
effect of neohesperidin dihydrochalcone on the activity and stability of alpha-amylase: a comparative study on bacterial, fungal, and mammalian enzymes.neohesperidin dihydrochalcone (nhdc) was recently introduced as an activator of mammalian alpha-amylase. in the current study, the effect of nhdc has been investigated on bacterial and fungal alpha-amylases. enzyme assays and kinetic analysis demonstrated the capability of nhdc to significantly activate both tested alpha-amylases. the ligand activation pattern was found to be more similar between the fungal and mammalian enzyme in comparison with the bacterial one. further, thermostability exper ...201525808616
aspergillus oryzae pathways that convert phenylalanine into the flavor volatile 2-phenylethanol.the filamentous fungus aspergillus oryzae rib40 produced 2-phenylethanol (pe) when cultured in minimum medium containing l-phenylalanine as a sole source of nitrogen. the fungus accumulated less pe in the absence of l-phenylalanine, indicating that it converted l-phenylalanine to pe. the pe production associated with fungal glucose consumption was repressed by exogenous ammonium, indicating that nitrogen-metabolite repression controls the pathway that produces pe. we identified the a. oryzae ppd ...201525797315
effective saccharification of kraft pulp by using a cellulase cocktail prepared from genetically engineered aspergillus oryzae.kraft pulp is a promising feedstock for bioproduction. the efficiency of kraft pulp saccharification was improved by using a cellulase cocktail prepared from genetically engineered aspergillus oryzae. application of the cellulase cocktail was demonstrated by simultaneous saccharification and fermentation, using kraft pulp and non-cellulolytic yeast. such application would make possible to do an efficient production of other chemicals from kraft pulp.201525783466
aspergillus oryzae fermented germinated soybean extract alleviates perimenopausal symptoms in ovariectomised rats.soybeans have been widely used to alleviate climacteric symptoms. in this study, we investigated the oestrogenic activities of isoflavones extracted from aspergillus oryzae-challenged germinated soybeans (ao-gs). eight-week-old virgin sprague-dawley female rats were ovariectomised (ovx). the rats were orally administered 0.1 mg kg(-1) 17α-ethinyl oestradiol or three different doses of ao-gs (0.5, 1.0 2.0 g kg(-1) day(-1)) in distilled water for 6 weeks, while control rats were administered vehic ...201625777819
cell biology of the koji mold aspergillus oryzae.koji mold, aspergillus oryzae, has been used for the production of sake, miso, and soy sauce for more than one thousand years in japan. due to the importance, a. oryzae has been designated as the national micro-organism of japan (koku-kin). a. oryzae has been intensively studied in the past century, with most investigations focusing on breeding techniques and developing methods for koji making for sake brewing. however, the understanding of fundamental biology of a. oryzae remains relatively lim ...201525776568
product identification of non-reducing polyketide synthases with c-terminus methyltransferase domain from talaromyces stipitatus using aspergillus oryzae heterologous expression.talaromyces stipitatus atcc 10500 possesses 17 non-reducing polyketide synthase (nr-pks) genes. during the course of our functional analysis of pks genes with a c-terminus methyltransferase domain from t. stipitatus, we expressed tspks2, tspks3 and tspks4 genes in the heterologous host aspergillus oryzae, respectively. although the tspks4 transformant gave no apparent product in hplc analysis, a novel azaphilone pentaketide (3) was identified along with two known related products from the tspks2 ...201525770780
drimane sesquiterpenoids from the aspergillus oryzae qxpc-4.three new drimane sesquiterpenoids, astellolides c-e (1-3, resp.), four new drimane sesquiterpenoid p-hydroxybenzoates, astellolides f-i (4-7, resp.), together with two known compounds astellolides a and b (8 and 9, resp.), have been isolated from the liquid culture of aspergillus oryzae (strain no. qxpc-4). their structures were established by comprehensive analysis of spectroscopic data. the relative and absolute configurations were determined on the basis of noesy and cd data, together with s ...201525766910
microbial bioconversion and processing methods enhance the phenolic acid and flavonoids and the radical scavenging capacity of smilax china l. has been reported that smilax china l. leaf (scl) provided various biological functions owing to polyphenols. the objective of the current study was to assess the enhancing effect of processing methods and microbial conversions on phenolic acid and flavonoid content and radical scavenging capacity of scl for potential applications of diverse food products.201625754764
cyanidin and malvidin in aqueous extracts of black carrots fermented with aspergillus oryzae prevent the impairment of energy, lipid and glucose metabolism in estrogen-deficient rats by ampk carrots (daucus carota l.) are rich in anthocyanins which contribute many health benefits, but are limited by bioavailability and instability when exposed to oxygen, heat and light. fermenting black carrots may improve the stability, absorption and bioactivity of its anthocyanins. here, we examined whether and by what mechanisms the long-term consumption of unfermented black carrot extract (bc) and its extracts fermented with lactobacillus plantarum (bclp) or aspergillus oryzae (bcao) migh ...201525701199
regulating yeast flavor metabolism by controlling saccharification reaction rate in simultaneous saccharification and fermentation of chinese maotai-flavor liquor.maotai-flavor liquor is produced by simultaneous saccharification and fermentation (ssf), in which filamentous fungi produce hydrolases to degrade the starch into fermentable sugar. saccharomyces cerevisiae simultaneously transforms the sugars to ethanol and flavor compounds. the saccharification rate plays an important role in regulating the liquor yield and flavor profile. this work investigated the effect of saccharification rate on fermentation by regulating the inoculation ratio (1:0.1, 1:0 ...201525676241
monitoring fungal growth on brown rice grains using rapid and non-destructive hyperspectral imaging.this research aimed to develop a rapid, non-destructive, and accurate method based on hyperspectral imaging (hsi) for monitoring spoilage fungal growth on stored brown rice. brown rice was inoculated with a non-pathogenic strain of aspergillus oryzae and stored at 30 °c and 85% rh. growth of a. oryzae on rice was monitored using viable colony counts, expressed as colony forming units per gram (cfu/g). the fungal development was observed using scanning electron microscopy. the hsi system was used ...201525662486
deep rna sequencing reveals a high frequency of alternative splicing events in the fungus trichoderma longibrachiatum.alternative splicing is crucial for proteome diversity and functional complexity in higher organisms. however, the alternative splicing landscape in fungi is still elusive.201525652134
computational study of β-n-acetylhexosaminidase from talaromyces flavus, a glycosidase with high substrate flexibility.β-n-acetylhexosaminidase (gh20) from the filamentous fungus talaromyces flavus, previously identified as a prominent enzyme in the biosynthesis of modified glycosides, lacks a high resolution three-dimensional structure so far. despite of high sequence identity to previously reported aspergillus oryzae and penicilluim oxalicum β-n-acetylhexosaminidases, this enzyme tolerates significantly better substrate modification. understanding of key structural features, prediction of effective mutants and ...201525627923
aspergillus oryzae s2 alpha-amylase production under solid state fermentation: optimization of culture conditions.aspergillus oryzae s2 was assayed for alpha-amylase production under solid state fermentation (ssf). in addition to amya and amyb already produced in monitored submerged culture, the strain was noted to produce new amyb oligomeric forms, in particular a dominant tetrameric form named amyc. the latter was purified to homogeneity through fractional acetone precipitation and size exclusion chromatography. sds-page and native page analyses revealed that, purified amyc was an approximately 172 kda te ...201525617840
purification and characterization of a thermostable hypothetical xylanase from aspergillus oryzae the current study, fermentation broth of aspergillus oryzae hml366 in sugar cane bagasse was subjected to ultrafiltration and ion exchange chromatography, and two xylanases, xynh1 and xynh2, were purified. time-of-flight mass spectrometry coupled with sds-page analysis revealed that xynh1 is identical to the hypothetical a. oryzae rib40 protein xp_001826985.1, with a molecular weight of 33.671 kda. likewise, xynh2 was identified as xylanase xynf1 with a molecular weight of 35.402 kda. sequenc ...201525604952
ionic interaction of positive amino acid residues of fungal hydrophobin rola with acidic amino acid residues of cutinase cutl1.hydrophobins are amphipathic proteins secreted by filamentous fungi. when the industrial fungus aspergillus oryzae is grown in a liquid medium containing the polyester polybutylene succinate co-adipate (pbsa), it produces rola, a hydrophobin, and cutl1, a pbsa-degrading cutinase. secreted rola attaches to the surface of the pbsa particles and recruits cutl1, which then condenses on the particles and stimulates the hydrolysis of pbsa. here, we identified amino acid residues that are required for ...201525588312
proteolysis in tempeh-type products obtained with rhizopus and aspergillus strains from grass pea (lathyrus sativus) seeds.tempeh is a food product obtained from legumes by means of solid-state fermentation with rhizopus sp. our previous research proved that mixed-culture inoculum may also be successfully applied. the objective of present research was to study the proteolytic activity of r. microsporus var. chinensis and a. oryzae during tempeh-type fermentation of grass pea seeds, and the effect of inoculum composition on the protein level and in vitro protein bioavailability in products.201728068010
cloning and expression of a novel prolyl endopeptidase from aspergillus oryzae and its application in beer stabilization.a novel prolyl endopeptidase gene from aspergillus oryzae was cloned and expressed in pichia pastoris. amino acid sequence analysis of the prolyl endopeptidase from aspergillus oryzae (ao-pep) showed that this enzyme belongs to a class serine peptide s28 family. expression, purification and characterization of ao-pep were analyzed. the optimum ph and temperature were ph 5.0 and 40 °c, respectively. the enzyme was activated and stabilized by metal ion ca(2+) and inhibited by zn(2+), mn(2+), al(3+ ...201525547787
development of aspergillus protease with ovalbumin-induced allergic chronic rhinosinusitis model in the mouse.chronic rhinosinusitis (crs) is a multifactorial inflammatory disease. particularly, eosinophilic crs is often recalcitrant to treatment, so an appropriate animal model is required to evaluate the pathogenesis of, and to develop therapies for, recalcitrant eosinophilic crs. this study aimed to improve the ovalbumin (ova)-induced mouse model of eosinophilic/allergic crs by combining ova with aspergillus protease, which is known to trigger allergic reactions in mouse lungs.201425514482
purification, cloning, characterization, and n-glycosylation analysis of a novel β-fructosidase from aspergillus oryzae fs4 synthesizing levan- and neolevan-type fructooligosaccharides.β-fructosidases are a widespread group of enzymes that catalyze the hydrolysis of terminal fructosyl units from various substrates. these enzymes also exhibit transglycosylation activity when they function with high concentrations of sucrose, which is used to synthesize fructooligosaccharides (fos) in the food industry. a β-fructosidase (bfra) with high transglycosylation activity was purified from aspergillus oryzae fs4 as a monomeric glycoprotein. compared with the most extensively studied asp ...201425501957
change in enzyme production by gradually drying culture substrate during solid-state fermentation.the influence of drying the culture substrate during solid-state fermentation on enzyme production was investigated using a non-airflow box. the drying caused a significant increase in enzyme production, while the mycelium content decreased slightly. this suggests that changes in the water content in the substrate during culture affect enzyme production in fungi.201525488496
distinct mechanism of activation of two transcription factors, amyr and malr, involved in amylolytic enzyme production in aspergillus oryzae.the production of amylolytic enzymes in aspergillus oryzae is induced in the presence of starch or maltose, and two zn2cys6-type transcription factors, amyr and malr, are involved in this regulation. amyr directly regulates the expression of amylase genes, and malr controls the expression of maltose-utilizing (mal) cluster genes. deletion of malr gene resulted in poor growth on starch medium and reduction in α-amylase production level. to elucidate the activation mechanisms of these two transcri ...201525487891
the ctng gene encodes carbonic anhydrase involved in mycotoxin citrinin biosynthesis from monascus aurantiacus.citrinin, a fungal secondary metabolite of polyketide origin, is moderately nephrotoxic to vertebrates, including humans. citrinin is synthesised by condensation of acetyl-coa and malonyl-coa. six genes involved in the citrinin biosynthesis, including pksct, ctna and ctnb, have been cloned in monascus purpureus. the pksct gene encodes a polyketide synthase; ctna is a regulatory factor; and ctnb encodes an oxidoreductase. when the three genes were respectively disrupted, the disruption strains dr ...201525482072
effect of the dose of exogenous fibrolytic enzyme preparations on preingestive fiber hydrolysis, ruminal fermentation, and in vitro digestibility of bermudagrass haylage.our objectives were to evaluate the effects of the dose rates of 5 trichoderma reesei and aspergillus oryzae exogenous fibrolytic enzymes (efe; 1a, 2a, 11c, 13d, and 15d) on in vitro digestibility, fermentation characteristics, and preingestive hydrolysis of bermudagrass haylage and to identify the optimal dose of each efe for subsequent in vitro and in vivo studies. in experiment 1, efe were diluted in citrate-phosphate buffer (ph 6) and applied in quadruplicate in each of 2 runs at 0× (control ...201525468699
molecular characterization of intergeneric hybrid between aspergillus oryzae and trichoderma harzianum by protoplast fusion.protoplast fusion between aspergillus oryzae and trichoderma harzianum and application of fusant in degradation of shellfish waste.201525444252
purification and characterisation of α-amylase produced by mutant strain of aspergillus oryzae ems-18.α-amylase produced by a mutant strain of aspergillus oryzae ems-18 has been purified to homogeneity as judged by sodium dodecyle sulphate polyacrylamide gel electrophoresis (sds-page). the enzyme was purified by using 70% ammonium sulphate precipitation followed by anion exchange chromatography on deae-sephadex column and gel filtration on sephadex g-100. an enzyme purification factor of 9.5-fold was achieved with a final specific activity of 1987.7 u/mg protein and overall yield of 23.8%. the m ...201525424747
characterisation of a recombinant β-xylosidase (xyla) from aspergillus oryzae expressed in pichia pastoris.β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. in this study we report the heterologous expression of aspergillus oryzae β-xylosidase (xyla) in pichia pastoris under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. the recombinant enzyme was optimally active at 55°c and ph 4.5 with km and vmax values of 1.0 mm and 250 μmol min(-1) mg(-1) respectively against 4-nitrophenyl β-xylopyranoside. xylose was a comp ...201425401069
modulating endoplasmic reticulum-golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus aspergillus oryzae.filamentous fungi are excellent hosts for industrial protein production due to their superior secretory capacity; however, the yield of heterologous eukaryotic proteins is generally lower than that of fungal or endogenous proteins. although activating protein folding machinery in the endoplasmic reticulum (er) improves the yield, the importance of intracellular transport machinery for heterologous protein secretion is poorly understood. here, using aspergillus oryzae as a model filamentous fungu ...201525362068
isolation and expression of enolase gene in fusarium oxysporum f. sp. lycopersici.fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. enolase genes have been isolated from bacteria and fungi, among other organisms. in this research, a large portion of the enolase, eno, gene sequence was isolated from f. oxysporum and compared with those of other microorganisms, revealing a similarity of 51-69 ...201525349089
characterization of the production of biogenic amines and gamma-aminobutyric acid in the soybean pastes fermented by aspergillus oryzae and lactobacillus brevis.the production of gamma-aminobutyric acid (gaba) using gaba-producing lactic acid bacteria (lab) has been considered to be an attractive strategy. however, some lab may produce biogenic amines (ba), which may be of concern from the safety viewpoint. the aim of the present study was to characterize the production of gaba and ba in the soybean pastes fermented by aspergillus oryzae (a. oryzae) fmb s46471 and gaba-producing lactobacillus brevis (l. brevis) gaba 100. after a ripening period of 90 da ...201525341471
evaluation of γ- aminobutyric acid, phytate and antioxidant activity of tempeh-like fermented oats (avena sativa l.) prepared with different filamentous fungi.tempeh is a popular traditional fermented food in asia. many tempeh-like foods are made from cereal grains. however, the information of γ-aminobutyric acid (gaba) accumulation in those tempeh-like cereal grains during fermentation is lacking. meanwhile, little information is available on the anti-nutrient contents and antioxidant activity of tempeh-like fermented oats. the aim of the present work was to study the changes of gaba, phytate, natural antioxidants and antioxidant activity of tempeh-l ...201425328194
l-lactic acid production from starch by simultaneous saccharification and fermentation in a genetically engineered aspergillus oryzae pure culture.lactic acid is a commodity chemical that can be produced biologically. lactic acid-producing aspergillus oryzae strains were constructed by genetic engineering. the a. oryzae ldh strain with the bovine l-lactate dehydrogenase gene produced 38 g/l of lactate from 100g/l of glucose. disruption of the wild-type lactate dehydrogenase gene in a. oryzae ldh improved lactate production. the resulting strain a. oryzae ldhδ871 produced 49 g/l of lactate from 100g/l of glucose. because a. oryzae strains i ...201425314668
effects of koji-making with mixed strains on physicochemical and sensory properties of chinese-type soy sauce.two kinds of soy sauces were prepared with aspergillus oryzae koji (ssao) and mixed koji (ssaom, a. oryzae mouldstarter:monascus purpureus mouldstarter = 1:2, w/w) respectively. the effects of mixed koji on the essential indices, oxygen radical absorption capacity, color indices, free amino acids and volatile compounds of soy sauce have been studied, followed by a sensory evaluation between ssao and ssaom.201525312834
development of a gin11/frt-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses.bioethanol produced by the yeast saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. however, hemicellulosic materials cannot be directly fermented by yeast: xylan backbone of hemicelluloses must first be hydrolyzed by heterologous hemicellulases to release xylose, and the yeast must then ferment xylose in the presenc ...201425306430
biosynthesis of the halogenated mycotoxin aspirochlorine in koji mold involves a cryptic amino acid conversion.aspirochlorine (1) is an epidithiodiketopiperazine (etp) toxin produced from koji mold (aspergillus oryzae), which has been used in the oriental cuisine for over two millennia. considering its potential risk for food safety, we have elucidated the molecular basis of aspirochlorine biosynthesis. by a combination of genetic and chemical analyses we found the acl gene locus and identified the key role of aclh as a chlorinase. stable isotope labeling, biotransformation, and mutational experiments, a ...201425302411
fungal hemicellulose-degrading enzymes cause physical property changes concomitant with solubilization of cell wall polysaccharides.physical properties of wheat coleoptile segments decreased after treatment with hemicellulose-degrading enzymes, indicating that hemicellulosic polysaccharides function to control the strength of primary cell walls. changes in the physical properties of plant cell walls, a viscoelastic structure, are thought to be one of the growth-limiting factors for plants and one of the infection-affecting factors for fungi. to study the significance of hemicellulosic polysaccharides that form cross-bridges ...201525301670
structure-activity relationship of highly potent galactonoamidine inhibitors toward β-galactosidase (aspergillus oryzae).a small library of 22 n-substituted galactonoamidines was synthesized, and their structure-activity relationship for inhibition of the hydrolytic activity of β-galactosidase (aspergillus oryzae) was evaluated. a fast screening assay in 96-well plate format was used to follow the enzymatic hydrolysis of 2-chloro-4-nitrophenyl-β-d-galactopyranoside using uv-vis spectroscopy. the aglycon moiety of all compounds was found to have a profound effect on their inhibitory ability. in general, galactonoam ...201425295392
identification of functional cis-elements required for repression of the taka-amylase a gene under secretion stress in aspergillus oryzae.the expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (ress). to investigate the ress mechanism, we analyzed the expression of the taka-amylase a gene (amyb) in aspergillus oryzae, which was depressed under secreted protein stress. we conducted a truncation and deletion analysis of the amyb promoter to identify cis-elements required for ress. a nucleotide sequ ...201525280730
involvement of hydrophobic amino acid residues in c7-c8 loop of aspergillus oryzae hydrophobin rola in hydrophobic interaction between rola and a polyester.hydrophobins are amphipathic secretory proteins with eight conserved cysteine residues and are ubiquitous among filamentous fungi. the cys3-cys4 and cys7-cys8 loops of hydrophobins are thought to form hydrophobic segments involved in adsorption of hydrophobins on hydrophobic surfaces. when the fungus aspergillus oryzae is grown in a liquid medium containing the polyester polybutylene succinate-co-adipate (pbsa), a. oryzae produces hydrophobin rola, which attaches to pbsa. here, we analyzed the k ...201425273133
engineering aspergillus oryzae a-4 through the chromosomal insertion of foreign cellulase expression cassette to improve conversion of cellulosic biomass into lipids.a genetic modification scheme was designed for aspergillus oryzae a-4, a natural cellulosic lipids producer, to enhance its lipid production from biomass by putting the spotlight on improving cellulase secretion. four cellulase genes were separately expressed in a-4 under the control of hlya promoter, with the help of the successful development of a chromosomal genetic manipulation system. comparison of cellulase activities of pcr-positive transformants showed that these transformants integrated ...201425251435
synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against trichophyton characterize and explore the potential in extracellular biosynthesis of silver nanoparticles (agnps) by penicillium chrysogenum and aspergillus oryzae and to investigate the antifungal effect of chemically vs biologically synthesized agnps comparing with conventional antifungal drugs against trichophyton rubrum.201425234047
establishment of a new method to quantitatively evaluate hyphal fusion ability in aspergillus oryzae.hyphal fusion is involved in the formation of an interconnected colony in filamentous fungi, and it is the first process in sexual/parasexual reproduction. however, it was difficult to evaluate hyphal fusion efficiency due to the low frequency in aspergillus oryzae in spite of its industrial significance. here, we established a method to quantitatively evaluate the hyphal fusion ability of a. oryzae with mixed culture of two different auxotrophic strains, where the ratio of heterokaryotic conidi ...201425229867
effects of fermented corni fructus and fermented kelp on growth performance, meat quality, and emission of ammonia and hydrogen sulphide from broiler chicken droppings.1. corni fructus is the fruit of cornus officinalis, a dogwood species. this study was conducted to prepare fermented corni fructus preparation (fcfp) and fermented kelp (fk) from corni fructus and by-products of laminaria japonica fermented with bacillus subtilis and aspergillus oryzae. 2. the effects of dietary fcfp and fk as replacer of oxytetracycline (otc) on growth performance, meat composition, meat oxidative stability, and emissions of ammonia (nh3) and hydrogen sulphide (h2s) from broil ...201425204489
phylogenetic and comparative sequence analysis of thermostable alpha amylases of kingdom archea, prokaryotes and eukaryotes.alpha amylase family is generally defined as a group of enzymes that can hydrolyse and transglycosylase α-(1, 4) or α-(1, 6) glycosidic bonds along with the preservation of anomeric configuration. for the comparative analysis of alpha amylase family, nucleotide sequences of seven thermo stable organisms of kingdom archea i.e. pyrococcus furiosus (100-105°c), kingdom prokaryotes i.e. bacillus licheniformis (90-95°c), geobacillus stearothermophilus (75°c), bacillus amyloliquefaciens (72°c), bacill ...201425187685
effects of aspergillus oryzae culture and 2-hydroxy-4-(methylthio)-butanoic acid on in vitro rumen fermentation and microbial populations between different roughage in vitro experiment was conducted to evaluate the effects of aspergillus oryzae culture (aoc) and 2-hydroxy-4-(methylthio)-butanoic acid (hmb) on rumen fermentation and microbial populations between different roughage sources. two roughage sources (chinese wild rye [cwr] vs corn silage [cs]) were assigned in a 2×3 factorial arrangement with hmb (0 or 15 mg) and aoc (0, 3, or 6 mg). gas production (gp), microbial protein (mcp) and total volatile fatty acid (vfa) were increased in response to a ...201425178372
biotransformation of the mycotoxin zearalenone by fungi of the genera rhizopus and aspergillus.zearalenone (zen) is a nonsteroidal estrogenic mycotoxin biosynthesized by various fusarium fungi. these fungal species frequently infest grains; therefore, zen represents a common contaminant in cereal products. the biotransformation of zen differs significantly from species to species, and several metabolites are known to be formed by animals, plants, and microorganisms. the aim of the present study was to investigate the microbial conversion of zen by species of the genera rhizopus and asperg ...201425145804
diversity in mrna expression of the serine-type carboxypeptidase ocpg in aspergillus oryzae through intron retention.alternative splicing is thought to be a means for diversification of products by mrna modification. although some intron retentions are predicted by transcriptome analysis in aspergillus oryzae, its physiological significance remains unknown. we found that intron retention occurred occasionally in the serine-type carboxypeptidase gene, ocpg. analysis under various culture conditions revealed that extracellular nitrogen conditions influence splicing patterns; this suggested that there might be a ...201425130734
c7-prenylation of tryptophanyl and o-prenylation of tyrosyl residues in dipeptides by an aspergillus terreus prenyltransferase.during our search for novel prenyltransferases, a putative gene ateg_04218 from aspergillus terreus raised our attention and was therefore amplified from strain dsm 1958 and expressed in escherichia coli. biochemical investigations with the purified recombinant protein and different aromatic substrates in the presence of dimethylallyl diphosphate revealed the acceptance of all the tested tryptophan-containing cyclic dipeptides. structure elucidation of the main enzyme products by nmr and ms anal ...201525125042
cloning and expression of a. oryzae β-glucosidase in pichia pastoris.a β-glucosidase gene (bgl) from aspergillus oryzae gif-10 was cloned, sequenced and expressed. its full-length dna sequence was 2,903 bp and included three introns. the full-length cdna sequence contained an open reading frame of 2,586 nucleotides, encoding 862 amino acids with a potential secretion signal. the a. oryzae gif-10 bgl was functionally expressed in pichia pastoris. after 7-day induction, protein yield reached 321 mg/ml. using salicin as the substrate, the specific activity of the pu ...201425123895
improvement of aspergillus oryzae nrrl 3484 by mutagenesis and optimization of culture conditions in solid-state fermentation for the hyper-production of extracellular cellulase.spore suspensions of aspergillus oryzae nrrl 3484 were subjected to mutagenesis using ultraviolet-irradiation followed by chemical treatments to improve the biosynthesis of cellulase. ten mutant strains namely ueac7, uear5, unac4, unac16, unar19, unbc7, unbr3, unbr10, unbr23 and unbr25 were selected and their extracellular cellulase activities were assayed. mutant unac4 gave the highest cellulase production [2,455 ± 28 u/g-dry substrate (ds) for filter paper-ase (fp-ase)] in a yield 4-fold excee ...201425119245
fungal lectins: a growing family.fungi are members of a large group of eukaryotic organisms that include yeasts and molds, as well as the most familiar member, mushrooms. fungal lectins with unique specificity and structures have been discovered. in general, fungal lectins are classified into specific families based on their amino acid sequences and three-dimensional structures. in this chapter, we provide an overview of the approximately 80 types of mushroom and fungal lectins that have been isolated and studied to date. in pa ...201425117221
optimization of the condition for adsorption of gallic acid by aspergillus oryzae mycelia using box-behnken design.fresh biomass of aspergillus oryzae (a. oryzae) cgmcc5992 can effectively remove gallic acid from aqueous solution. to improve the removal rate of gallic acid, this study first identified the important factors affecting the removal rate of gallic acid with univariate analysis, and then used four-factor and three-level box-behnken design (bbd) with the removal rate of gallic acid as response value, to obtain the optimum conditions for the removal of gallic acid as follows: 6.95 h treatment time, ...201525109471
a matter of structure: structural comparison of fungal carbonic anhydrases.carbonic anhydrases (cas) are metalloenzymes that catalyze the interconversion of carbon dioxide (co2) and hydrogen carbonate. cas are distributed over all the three domains of life and are divided into five distinct evolutionarily unrelated gene families (α, β, γ, δ, ζ). in the large fungal kingdom, the majority of fungi encode multiple copies of β-cas, with some also possessing genes for α-class cas. hemiascomycetous and basidiomycetous yeasts encode one or two β-cas, while most of the filamen ...201425109265
[the effect of enzymatic treatment using proteases on properties of persistent sodium current in ca1 pyramidal neurons of rat hippocampus].we investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (i(nap)) in hippocampal ca1 pyramidal neurons. properties of i(nap) were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase e (from streptomyces griseus) or protease type xxiii (from aspergillus oryzae). we show that in neurons isolated with pron ...201425097934
rapid reconstitution of biosynthetic machinery for fungal metabolites in aspergillus oryzae: total biosynthesis of aflatrem.reconstitution of the biosynthetic machinery for fungal secondary metabolites in aspergillus oryzae provides an opportunity both for stepwise determination of the biosynthetic pathways and the total biosynthesis of fungal natural products. however, to maximize the utility of the reconstitution system, a simple and rapid strategy for the introduction of heterologous genes into a. oryzae is required. in this study, we demonstrated an effective method for introducing multiple genes involved in the ...201425087641
expression of key hydrolases for soy sauce fermentation in zygosaccharomyces rouxii.several key hydrolases in soy sauce fermentation such as proteases, peptidases, and glutaminases are supplied by aspergillus sojae or aspergillus oryzae. the genes encoding these hydrolases were successfully expressed in salt-tolerant yeast zygosaccharomyces rouxii. these transformants are expected to supply extra hydrolases during soy sauce fermentation process.201525073685
crystal structure of a feruloyl esterase belonging to the tannase family: a disulfide bond near a catalytic triad.feruloyl esterase (fae) catalyzes the hydrolysis of the ferulic and diferulic acids present in plant cell wall polysaccharides, and tannase catalyzes the hydrolysis of tannins to release gallic acid. the fungal tannase family in the esther database contains various enzymes, including faes and tannases. despite the importance of faes and tannases in bioindustrial applications, three-dimensional structures of the fungal tannase family members have been unknown. here, we determined the crystal stru ...201425066066
gas chromatography/mass spectrometry based component profiling and quality prediction for japanese sake.sake is a japanese traditional alcoholic beverage, which is produced by simultaneous saccharification and alcohol fermentation of polished and steamed rice by aspergillus oryzae and saccharomyces cerevisiae. about 300 compounds have been identified in sake, and the contribution of individual components to the sake flavor has been examined at the same time. however, only a few compounds could explain the characteristics alone and most of the attributes still remain unclear. the purpose of this st ...201425060729
effects of dietary fermented seaweed and seaweed fusiforme on growth performance, carcass parameters and immunoglobulin concentration in broiler chicks.this study was conducted to investigate the effects of brown seaweed (undaria pinnatifida) by-product and seaweed fusiforme (hizikia fusiformis) by-product supplementation on growth performance and blood profiles including serum immunoglobulin (ig) in broilers. fermentation of seaweeds was conducted by bacillus subtilis and aspergillus oryzae. in a 5-wk feeding trial, 750 one-d-old broiler chicks were divided into 5 groups, and were assigned to the control diet or experimental diets including co ...201425050025
comparison of the genomes and transcriptomes associated with the different protease secretions of aspergillus oryzae 100-8 and 3.042.aspergillus oryzae is used to produce traditional fermented foods and beverages. a. oryzae 3.042 produces a neutral protease and an alkaline protease but rarely an acid protease, which is unfavourable to soy-sauce fermentation. a. oryzae 100-8 was obtained by n(+) ion implantation mutagenesis of a. oryzae 3.042, and the protease secretions of these two strains are different. sequencing the genome of a. oryzae 100-8 and comparing it to the genomes of a. oryzae 100-8 and 3.042 revealed some differ ...201425048221
a combination of acid lactase from aspergillus oryzae and yogurt bacteria improves lactose digestion in lactose maldigesters synergistically: a randomized, controlled, double-blind cross-over trial.lactose digestion can be improved in subjects with impaired or completely absent intestinal lactase activity by administration of lactase preparations and particularly of acid lactase, which is active in the stomach, or by yogurt containing live lactic acid bacteria. it is the question, if lactose digestion can be further enhanced by combining these two approaches.201525042846
trichoderma reesei xyn vi--a novel appendage-dependent eukaryotic glucuronoxylan hydrolase.expression of a trichoderma reesei gene coding for a putative gh30 xylanase in aspergillus oryzae led to isolation and purification of a novel xylanase exhibiting catalytic properties different from those of the previously characterized gh30 xylanase xyn iv of t. reesei. the novel enzyme, named xyn vi, exhibited catalytic properties similar to appendage-dependent gh30 glucuronoxylanases previously recognized only in bacteria. xyn vi showed high specific activity only on xylans or xylooligosaccha ...201425041335
plant-available inorganic nutrient levels are increased in rice-derived distillery effluents inoculated with microbes.the purpose of this study was to assess the effects of microbes on plant-available inorganic nutrients and a phytohormone in rice-derived distillery effluents.201425040155
targeted metabolomics for aspergillus oryzae-mediated biotransformation of soybean isoflavones, showing variations in primary metabolites.this study aimed to investigate the biotransformation of soybean isoflavones to hydroxyisoflavones, and the primary and secondary metabolite change during aspergillus oryzae kacc40247-mediated fermentation by gas chromatography-time of flight-mass spectrometry and lc-ms with multivariate analysis. the mass spectrometric analysis revealed that acetylglycosides and glycosides decreased during the first 12 h of fermentation, while the aglycones increased up to that time point. this was followed by ...201425036500
telomere-mediated chromosomal truncation in aspergillus oryzae.we truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in aspergillus oryzae. the predicted deletion was confirmed by southern blot analyses. this telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in a. oryzae.201525034635
a comparative study on the degradation of gallic acid by aspergillus oryzae and phanerochaete chrysosporium.recently, as an emerging persistent dissolved organic pollutant (dop), gallic acid (ga) and its efficient decomposition methods have received global attention. the present work aimed to compare the effect of aspergillus oryzae 5992 and phanerochaete chrysosporium 40719 on degradation of different concentrations of ga. the a. oryzae grew well and achieved a ga removal rate up to 99% in media containing 1-4% ga, much higher than p. chrysosporium. the activity of laccase and lignin peroxidase excre ...201425026596
[gene cloning, expression of a feruloyl esterase a and purification of its hydrolysis products].to express feruloyl esterase a from aspergillus oryzae in pichia pastoris expression system and study its hydrolysis function, explore the conditions and effects of purification for ferulic acid extracts by macroporos resin. using the total rna from a. oryzae cicc 40186 as the template, we amplified coding sequence aorfaea encoding a mature feruloyl esterase a (aorfaea) by rt-pcr technique. then, the coding sequence aorfaea was successfully expressed in pichia pastoris gs115 mediated by an expre ...201425007578
assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant aspergillus oryzae.anhydromevalonolactone (amvl) is a bioactive natural product that arises from a molecular biology technique using aspergillus oryzae as a heterologous host. amvl has been used as a precursor for the synthesis of insect pest control reagents and has numerous applications in the biotechnological and medical industries. in this study, the plackett-burman design and the central composite design, which offer efficient and feasible approaches, were complemented to screen significant parameters and ide ...201425006541
safety evaluation of filamentous fungi isolated from industrial doenjang koji.a few starters have been developed and used for doenjang fermentation but often without safety evaluation. filamentous fungi were isolated from industrial doenjang koji, and their potential for mycotoxin production was evaluated. two fungi were isolated; one was more dominantly present (90%). both greenish (snu-g) and whitish (snu-w) fungi showed 97% and 95% internal transcribed spacer sequence identities to aspergillus oryzae/flavus, respectively. however, the smai digestion pattern of their ge ...201424986679
sol-gel immobilization as a suitable technique for enhancement of α-amylase activity of aspergillus oryzae pp.bioencapsulation of microbial cells in silica-based matrices has proved to be a good strategy to enhance the biosynthetic capabilities and viability of bioproducers. in the present study, mycelium and pellet cultures of strain aspergillus oryzae pp were successfully immobilized in sol-gel hybrid matrices composed of tetraethylorthosilicate as an inorganic precursor, 5% (w/v) starch and 10 or 15% (w/v) polyethylene oxide, or 10% (w/v) calcium alginate as organic compounds. biosynthetic activity o ...201426740773
speradines f-h, three new oxindole alkaloids from the marine-derived fungus aspergillus oryzae.a rare hexacyclic oxindole alkaloid, speradine f (1), together with two novel tetracyclic oxindole alkaloids, speradines g (2) and h (3), were isolated from the marine-derived fungus aspergillus oryzae. their structures were determined by spectroscopic methods and x-ray diffraction analysis. this study is the first report on cyclopiazonic acid (cpa)-type alkaloids with a hexacyclic skeleton.201424966178
supplementation with aspergillus oryzae-fermented kochujang lowers serum cholesterol in subjects with hyperlipidemia.kochujang, a traditional fermented red pepper paste, is known for its hypocholesterolemic effect; however, these studies used non-commercial preparations of kochujang. in this study, we examined whether commercially-made kochujang in which aspergillus oryzae (also known as koji) was used as a microorganism for fermentation has the same cholesterol-lowering effects.201524961447
regioselective synthesis of cytarabine monopropionate by using a fungal whole-cell biocatalyst in nonaqueous medium.the utilization of a dehydrated fungal biocatalyst of aspergillus oryzae cells was successfully performed to achieve efficient acylation modification of a polar nucleoside cytarabine (ara-c). organic solvents showed evident influence on the reaction catalyzed by the a. oryzae whole-cells. except for hexane-pyridine, the catalytic activity and regioselectivity of the whole-cells clearly increased with increasing the polarity of the hydrophobic organic solvents used. the effects of some crucial fa ...201424957351
biochemical properties of a new cold-active mono- and diacylglycerol lipase from marine member janibacter sp. strain htcc2649.mono- and di-acylglycerol lipase has been applied to industrial usage in oil modification for its special substrate selectivity. until now, the reported mono- and di-acylglycerol lipases from microorganism are limited, and there is no report on the mono- and di-acylglycerol lipase from bacteria. a predicted lipase (named maj1) from marine janibacter sp. strain htcc2649 was purified and biochemical characterized. maj1 was clustered in the family i.7 of esterase/lipase. the optimum activity of the ...201424927145
expression, purification and crystallization of a fungal type iii polyketide synthase that produces the csypyrones.csyb from aspergillus oryzae is a novel type iii polyketide synthase that catalyzes the formation of csypyrone b1 [4-(3-acetyl-4-hydroxy-2-oxo-2h-pyran-6-yl)butyric acid] from fatty acyl-coa, malonyl-coa and acetoacetyl-coa. recombinant csyb expressed in escherichia coli was crystallized by the sitting-drop vapour-diffusion method. the crystals belonged to space p2₁, with unit-cell parameters a=70.0, b=104.8, c=73.5 å, β=114.4°.201424915080
draft genome sequence of aspergillus oryzae 100-8, an increased acid protease production strain.aspergillus oryzae is a common fungus for traditional fermentation in asia, such as spirit, soybean paste, and soy sauce fermentation. we report the 36.7-mbp draft genome sequence of a. oryzae 100-8 and compared it to the published genome sequence of a. oryzae 3.042.201424903875
aspergillus oryzae csyb catalyzes the condensation of two β-ketoacyl-coas to form 3-acetyl-4-hydroxy-6-alkyl-α-pyrone.the type iii polyketide synthases from fungi produce a variety of secondary metabolites including pyrones, resorcinols, and resorcylic acids. we previously reported that csyb from aspergillus oryzae forms α-pyrone csypyrone b compounds when expressed in a. oryzae. feeding experiments of labeled acetates indicated that a fatty acyl starter is involved in the reaction catalyzed by csyb. here we report the in vivo and in vitro reconstitution analysis of csyb. when csyb was expressed in escherichia ...201424895122
aspergillus oryzae-based cell factory for direct kojic acid production from cellulose.kojic acid (5-hydroxy-2-(hydroxymethyl)-4-pyrone) is one of the major secondary metabolites in aspergillus oryzae. it is widely used in food, pharmaceuticals, and cosmetics. the production cost, however, is too high for its use in many applications. thus, an efficient and cost-effective kojic acid production process would be valuable. however, little is known about the complete set of genes for kojic acid production. currently, kojic acid is produced from glucose. the efficient production of koj ...201424885968
phytase production by aspergillus oryzae in solid-state fermentation and its applicability in dephytinization of wheat bran [corrected].aspergillus oryzae sbs50 secreted a high titre of phytase in solid-state fermentation (ssf) using wheat bran at 30 °c after 96 h at the initial substrate to moisture ratio of 1:2 and a water activity of 0.95. the production of phytase increased when wheat bran was supplemented with sucrose and beef extract. further enhancement in enzyme production was recorded when the substrate was supplemented with the surfactant triton x-100 (145 u/g of dmb). an overall 29-fold improvement in phytase producti ...201424879597
over-expression of a proline specific aminopeptidase from aspergillus oryzae jn-412 and its application in collagen degradation.a strain that exhibited intracellular proline-specific aminopeptidase (pap) activity was isolated from soy sauce koji and identified as aspergillus oryzae jn-412. the gene coding pap was cloned and efficiently expressed in escherichia coli bl21 in a biologically active form. the highest specific activity reached 52.28 u mg(-1) at optimum cultivation conditions. the recombinant enzyme was purified 3.3-fold to homogeneity with a recovery of 36.7% from cell-free extract using ni-affinity column chr ...201424879594
biocontrol activity of the local strain of metschnikowia pulcherrima on different postharvest pathogens.the strains of the yeast metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. biocontrol activity of m. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. biocontrol activity of pulcherrimin producing strain, m. pulcherrima umy15, isolated from local vineyards, was tested on different molds that cause food spoilage. m. pulcherrima umy15 was a very effective biocontrol agent against penicillium roqueforti, p. italicum, p. expansum, and ...201424860671
biochemical characterization of aspergillus oryzae native tannase and the recombinant enzyme expressed in pichia this study, the biochemical properties of the recombinant tannase from aspegillus oryzae were compared with those of the native enzyme. extracellular native tannase was purified from a commercial enzyme source. recombinant tannase highly expressed in pichia pastoris was prepared as an active extracellular protein. purified native and recombinant tannases produced smeared bands with apparent molecular masses of 45-80 kda and 45-75 kda, respectively, by sodium dodecyl sulfate-polyacrylamide gel ...201424856589
targeted tandem duplication of a large chromosomal segment in aspergillus oryzae.we describe here the first successful construction of a targeted tandem duplication of a large chromosomal segment in aspergillus oryzae. the targeted tandem chromosomal duplication was achieved by using strains that had a 5'-deleted pyrg upstream of the region targeted for tandem chromosomal duplication and a 3'-deleted pyrg downstream of the target region. consequently,strains bearing a 210-kb targeted tandem chromosomal duplication near the centromeric region of chromosome 8 and strains beari ...201424837372
dry entrapment of enzymes by epoxy or polyester resins hardened on different solid supports.embedding of enzymes was performed with epoxy or polyester resin by mixing in a dried enzyme preparation before polymerization was started. this fast and low-cost immobilization method produced enzymatically active layers on different solid supports. as model enzymes the well-characterized thermomyces lanuginosus lipase and a new threonine aldolase from ashbya gossypii were used. it was shown that t. lanuginosus lipase recombinantly expressed in aspergillus oryzae is a monomeric enzyme with a mo ...201424835099
a large nonconserved region of the tethering protein leashin is involved in regulating the position, movement, and function of woronin bodies in aspergillus oryzae.the woronin body is a pezizomycotina-specific organelle that is typically tethered to the septum, but upon hyphal wounding, it plugs the septal pore to prevent excessive cytoplasmic loss. leashin (lah) is a large woronin body tethering protein that contains highly conserved n- and c-terminal regions and a long (∼2,500-amino-acid) nonconserved middle region. as the involvement of the nonconserved region in woronin body function has not been investigated, here, we functionally characterized indivi ...201424813188
filamentous fungal diversity and community structure associated with the solid state fermentation of chinese maotai-flavor liquor.maotai-flavor liquor is produced by simultaneous saccharification and fermentation (ssf) process under solid state conditions, including daqu (starter) making, stacking fermentation and alcohol fermentation stages. filamentous fungi produce many enzymes to degrade the starch material into fermentable sugar during liquor fermentation. this study investigated the filamentous fungal community associated with liquor making process. eight and seven different fungal species were identified by using cu ...201424742997
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