chirally pure prodrugs and their converting enzymes lead to high supersaturation and rapid transcellular permeation of benzodiazepines.water-soluble prodrugs can be rapidly converted by enzymes to hydrophobic drugs, whose aqueous thermodynamic solubilities are low, but are maintained in aqueous solution at supersaturated concentrations due to slow precipitation kinetics. recently, we investigated avizafone (avf) in combination with aspergillus oryzae protease as a prodrug/enzyme system intended to produce supersaturated diazepam (dzp). several fold enhancement of permeation of supersaturated dzp across madin-darby canine kidney ...201627342435
potential pitfalls associated with testing of enzyme preparations in the salmonella/microsome assay.the effect of a sample of food enzyme preparations on s9 activity was evaluated in bacterial mutation assays with the salmonella typhimurium strains ta98 and ta100 using benzo(a)pyrene, 2-aminoanthracene and 2-aminofluorene as model compounds. under the experimental conditions applied, aspergillus oryzae protease and porcine pancreas trypsin, applied at low non-toxic doses, proved to effectively inhibit the metabolic activation of benzo(a)pyrene by aroclor induced rat liver 9, while the activati ...201627330008
six independent fucose-binding sites in the crystal structure of aspergillus oryzae lectin.the crystal structure of aol (a fucose-specific lectin of aspergillus oryzae) has been solved by sad (single-wavelength anomalous diffraction) and mad (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. the overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. the fucose moieties of the seleno-fucosides are located in six fucose-binding sites. although the arg and glu/gln residues bound to the fucose moiety are common to all fucose-bind ...201627318092
metabolomic profiles of aspergillus oryzae and bacillus amyloliquefaciens during rice koji fermentation.rice koji, used early in the manufacturing process for many fermented foods, produces diverse metabolites and enzymes during fermentation. using gas chromatography time-of-flight mass spectrometry (gc-tof-ms), ultrahigh-performance liquid chromatography linear trap quadrupole ion trap tandem mass spectrometry (uhplc-ltq-it-ms/ms), and multivariate analysis we generated the metabolite profiles of rice koji produced by fermentation with aspergillus oryzae (rk_ao) or bacillus amyloliquefaciens (rk_ ...201627314317
a novel organic solvent- and detergent-stable serine alkaline protease from trametes cingulata strain ctm10101.a protease-producing fungus was isolated from an alkaline wastewater of chemical industries and identified as trametes cingulata strain ctm10101 on the basis of the its rdna gene-sequencing. it was observed that the fungus strongly produce extracellular protease grown at 30°c in potato-dextrose-broth (pdb) optimized media (13500u/ml). the pure serine protease isolated by trametes cingulata (designated sptc) was purified by ammonium sulfate precipitation-dialysis followed by heat-treatment and un ...201627296442
production and biochemical characterization of an alkaline protease from aspergillus oryzae this study, aspergillus oryzae ch93 was isolated from soil sample and examined using molecular analysis. following culture of a. oryzae ch93 under optimal enzyme production, a 47.5kda extracellular protease was purified using ammonium sulfate precipitation and q-sepharose chromatography. the optimal ph 8 and temperature of 50°c obtained for the isolated protease. sodium dodecyl sulfate (sds), cetyltrimethyl ammonium bromide (ctab), h2o2 decreased activity, while triton x-100 and phenylmethane ...201727293035
aberrant glycosylation in the left ventricle and plasma of rats with cardiac hypertrophy and heart failure.targeted proteomics focusing on post-translational modifications, including glycosylation, is a useful strategy for discovering novel biomarkers. to apply this strategy effectively to cardiac hypertrophy and resultant heart failure, we aimed to characterize glycosylation profiles in the left ventricle and plasma of rats with cardiac hypertrophy. dahl salt-sensitive hypertensive rats, a model of hypertension-induced cardiac hypertrophy, were fed a high-salt (8% nacl) diet starting at 6 weeks. as ...201627281159
development of a promoter shutoff system in aspergillus oryzae using a sorbitol-sensitive promoter.promoter shutoff is a general method for analyzing essential genes, but in the fungus aspergillus oryzae, no tightly repressed promoters have been reported. to overcome the current limitations of conditional promoters, we examined sorbitol- and galactose-responsive genes using microarrays to identify regulatable genes with only minor physiological and genetic effects. we identified two sorbitol-induced genes (designated as sora and sorb), cloned their promoters, and built a regulated egfp and br ...201627280333
sequence- and structure-based functional annotation and assessment of metabolic transporters in aspergillus oryzae: a representative case study.aspergillus oryzae is widely used for the industrial production of enzymes. in a. oryzae metabolism, transporters appear to play crucial roles in controlling the flux of molecules for energy generation, nutrients delivery, and waste elimination in the cell. while the a. oryzae genome sequence is available, transporter annotation remains limited and thus the connectivity of metabolic networks is incomplete. in this study, we developed a metabolic annotation strategy to understand the relationship ...201627274991
modeling lactose hydrolysis for efficiency and selectivity: toward the preservation of sialyloligosaccharides in bovine colostrum whey permeate.enzymatic hydrolysis of lactose has been shown to improve the efficiency and selectivity of membrane-based separations toward the recovery of bioactive oligosaccharides. achieving maximum lactose hydrolysis requires intrinsic process optimization for each specific substrate, but the effects of those processing conditions on the target oligosaccharides are not well understood. response surface methodology was used to investigate the effects of ph (3.25-8.25), temperature (35-55°c), reaction time ...201627236766
nematicidal activity of kojic acid produced by aspergillus oryzae against meloidogyne incognita.the fungal strain eml-dml3pna1 isolated from leaf of white dogwood (cornus alba l.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. the active fungal strain was identified as aspergillus oryzae, which belongs to section flavi, based on the morphological characteristics and sequence analysis of the its rdna, calmodulin (cam), and β-tubulin (bena) genes. the strain reduced the ph value to 5.62 afte ...201627197670
development of enzyme technology for aspergillus oryzae, a. sojae, and a. luchuensis, the national microorganisms of japan.this paper describes the modern enzymology in japanese bioindustries. the invention of takadiastase by jokiti takamine in 1894 has revolutionized the world of industrial enzyme production by fermentation. in 1949, a new γ-amylase (glucan 1,4-α-glucosidase, ec from a. luchuensis (formerly designated as a. awamori), was found by kitahara. rnase t1 (guanyloribonuclease, ec was discovered by sato and egami. ando discovered aspergillus nuclease s1 (single-stranded nucleate endonucl ...201627151561
identification and manipulation of the pleuromutilin gene cluster from clitopilus passeckerianus for increased rapid antibiotic production.semi-synthetic derivatives of the tricyclic diterpene antibiotic pleuromutilin from the basidiomycete clitopilus passeckerianus are important in combatting bacterial infections in human and veterinary medicine. these compounds belong to the only new class of antibiotics for human applications, with novel mode of action and lack of cross-resistance, representing a class with great potential. basidiomycete fungi, being dikaryotic, are not generally amenable to strain improvement. we report identif ...201627143514
highly efficient fructooligosaccharides production by an erythritol-producing yeast yarrowia lipolytica displaying fructosyltransferase.currently, fructooligosaccharides (fos) are industrially transformed from sucrose by purified enzymes or fungi cells. however, these methods are expensive and time-consuming. an economical approach to producing fos using erythritol-producing yeast cells was described in this study. fructosyltransferase from aspergillus oryzae was displayed on the cell surface of yarrowia lipolytica, resulting in an engineered strain capable of transforming sucrose to fos. an amount of 480 g/l fos was produced wi ...201627124471
structural elucidation of enzymatically synthesized galacto-oligosaccharides using ion-mobility spectrometry-tandem mass spectrometry.galacto-oligosaccharides (gos) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with β-galactosidases. enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different ty ...201627109424
molecular dissection of so (soft) protein in stress-induced aggregation and cell-to-cell interactive functions in filamentous fungal multicellularity.filamentous fungi grow by organizing multicellularity through hyphal compartmentalization and cell fusion. so (soft) protein, which was originally identified in neurospora crassa, plays distinct functional roles in cell-to-cell interactions, such as septal plugging and cell fusion. we previously reported that aoso, an aspergillus oryzae so homologue, forms aggregates at the septal pore in response to stress, as well as upon hyphal wounding. however, the functional regions that mediate the multic ...201627109373
aspergillus oryzae nrta affects kojic acid production.we analyzed the role of the nitrate transporter-encoding gene (nrta) of aspergillus oryzae by gene disruption. southern hybridization analysis indicated that homologous recombination occurred at the resident nrta locus. real-time pcr showed that the nrta gene was strongly inducible by nano3. the nrta disruptant did not exhibit normal growth when nitrate was available as the sole nitrogen source. these results indicate that nrta is essential for nitrate uptake in a. oryzae. kojic acid (ka) produc ...201627108780
aspergillus oryzae s2 α-amylase domain c involvement in activity and specificity: in vivo proteolysis, molecular and docking studies.we previously reported that aspergillus oryzae strain s2 had produced two α-amylase isoforms named amya and amyb. the apparent molecular masses revealed by sds-page were 50 and 42 kda, respectively. yet amyb has a higher catalytic efficiency. based on a monitoring study of the α-amylase production in both the presence and absence of different protease inhibitors, a chymotrypsin proteolysis process was detected in vivo generating amyb. a. oryzae s2 α-amylase gene was amplified, cloned and sequenc ...201627101008
an alternative approach to synthesizing galactooligosaccharides by cell-surface display of β-galactosidase on yarrowia alternative strategy for synthesizing galactooligosaccharides (gos) from an erythritol-producing yeast yarrowia lipolytica using surface display technology was demonstrated. the engineered strain cgmcc11369 was developed by fusion of the β-galactosidase gene from aspergillus oryzae to the ylpir1 gene, which codes for a cell wall protein. β-galactosidase was effectively displayed on the cell surface of yarrowia lipolytica start strain cgmcc7326. this engineered strain with surface-displayed β- ...201627090877
production of alpha-amylase from aspergillus oryzae for several industrial applications in a single step.a one-step method as a strategy of alpha-amylase concentration and purification was developed in this work. this methodology requires the use of a very low concentration of biodegradable polyelectrolyte (eudragit(®) e-po) and represents a low cost, fast, easy to scale up and non-polluting technology. besides, this methodology allows recycling the polymer after precipitation. the formation of reversible soluble/insoluble complexes between alpha-amylase and the polymer eudragit(®) e-po was studied ...201627085017
biotransformation of l-tyrosine to dopamine by a calcium alginate immobilized mutant strain of aspergillus oryzae.the present research work is concerned with the biotransformation of l-tyrosine to dopamine (da) by calcium alginate entrapped conidiospores of a mutant strain of aspergillus oryzae. different strains of a. oryzae were isolated from soil. out of 13 isolated strains, isolate-2 (i-2) was found to be a better da producer. the wild-type i-2 was chemically improved by treating it with different concentrations of ethyl methyl sulfonate (ems). among seven mutant variants, ems-6 exhibiting maximal da ac ...201627068833
a novel non-thermostable deuterolysin from aspergillus oryzae.three putative deuterolysin (ec genes (deua, deub, and deuc) were found in the aspergillus oryzae genome database ( ). one of these genes, deua, was corresponding to npii gene, previously reported. deua and deub were overexpressed by recombinant a. oryzae and were purified. the degradation profiles against protein substrates of both enzymes were similar, but deub showed wider substrate specificity against peptidyl mca-substrates compared ...201627050120
astellifadiene: structure determination by nmr spectroscopy and crystalline sponge method, and elucidation of its biosynthesis.genome mining of a terpene synthase gene from emericella variecolor nbrc 32302 and its functional expression in aspergillus oryzae led to the production of the new sesterterpene hydrocarbon, astellifadiene (1), having a 6-8-6-5-fused ring system. the structure of 1 was initially investigated by extensive nmr analyses, and was further confirmed by the crystalline sponge method, which established the absolute structure of 1 and demonstrated the usefulness of the method in the structure determinati ...201627038368
enhancement of fructosyltransferase and fructooligosaccharides production by a. oryzae dia-mf in solid-state fermentation using aguamiel as culture medium.the aim of this work was to improve the production of fructosyltransferase (ftase) by solid-state fermentation (ssf) using aguamiel (agave sap) as culture medium and aspergillus oryzae dia-mf as producer strain. ssf was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial ph and packing density to determine the most significant factors through box-hunter and hunter design. the significant factors were then further optimized using a box-behnken design an ...201627036329
synthesis of oligosaccharides derived from lactulose (oslu) using soluble and immobilized aspergillus oryzae β-galactosidase.β-galactosidase from aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (oslu) by transgalactosylation. oligosaccharides with degree of polymerization (dp) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. thus, in this work, an optimization of reaction conditions affecting the specific selectivity of a. oryzae β-galactosidase for synthesis of oslu has been carried out to enhance oslu with dp ≥ 3 productio ...201627014684
further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in aspergillus oryzae faaa fatty acids are useful as source materials for the production of biodiesel fuel and various chemicals such as pharmaceuticals and dietary supplements. previously, we attained a 9.2-fold increase in free fatty acid productivity by disrupting a predicted acyl-coa synthetase gene (faaa, ao090011000642) in aspergillus oryzae. in this study, we achieved further increase in the productivity by overexpressing a predicted transketolase gene of the pentose phosphate pathway in the faaa disruptant. t ...201626998626
substantial decrease in cell wall α-1,3-glucan caused by disruption of the kexb gene encoding a subtilisin-like processing protease in aspergillus oryzae.disruption of the kexb encoding a subtilisin-like processing protease in aspergillus oryzae (δkexb) leads to substantial morphological defects when the cells are grown on czapek-dox agar plates. we previously found that the disruption of kexb causes a constitutive activation of the cell wall integrity pathway. to understand how the disruption of the kexb affects cell wall organization and components, we analyzed the cell wall of δkexb grown on the plates. the results revealed that both total n-a ...201626980104
the c2h2-type transcription factor, flbc, is involved in the transcriptional regulation of aspergillus oryzae glucoamylase and protease genes specifically expressed in solid-state culture.aspergillus oryzae produces a large amount of secreted proteins in solid-state culture, and some proteins such as glucoamylase (glab) and acid protease (pepa) are specifically produced in solid-state culture, but rarely in submerged culture. from the disruption mutant library of a. oryzae transcriptional regulators, we successfully identified a disruption mutant showing an extremely low production level of glab but a normal level of α-amylase production. this strain was a disruption mutant of th ...201626960315
performance of an ultrafiltration membrane bioreactor (uf-mbr) as a processing strategy for the synthesis of galacto-oligosaccharides at high substrate concentrations.the performance of an ultrafiltration membrane bioreactor for galacto-oligosaccharides (gos) synthesis using high lactose concentrations (470 g/l) and β-galactosidase from aspergillus oryzae was assessed. tested processing variables were: transmembrane-pressure (pt), crossflow-velocity (cfv) and temperature. results showed that processing variables had significant effect on the yield, the enzyme productivity and the flux but did not on gos concentration and reaction conversion obtained. as expec ...201626924238
homology modeling and molecular dynamics study on schwanniomyces occidentalis alpha-amylase.with consumers growing increasingly aware of environmental issues, industries find enzymes as a reasonable alternative over physical conditions and chemical catalysts. amylases are important hydrolase enzymes, which have been widely used in variety of industrial process such as pharmaceutical, food, and fermentation industries. among amylases α-amylase is in maximum demand due to its wide range of applications. the homology modeling study on schwanniomyces occidentalis amylase (amy1, uniprot ide ...201726882245
molecular cloning and characterization of a novel bi-functional α-amylase/subtilisin inhibitor from hevea brasiliensis.a novel cdna encoding a bi-functional α-amylase/subtilisin inhibitor (hbasi) was isolated from rubber (hevea brasiliensis) leaves cultivar rrim600. the hbasi had strong homology with the soybean trypsin inhibitor (kunitz) family of protease inhibitors. its putative amino acid sequence was similar to that of the α-amylase/subtilisin inhibitor from ricinus communis (72% identity). genomic sequencing indicated that the hbasi gene contained no introns. the messenger rna of hbasi was detected in leaf ...201626854410
efficient kinetic resolution of (rs)-1-phenylethanol by a mycelium-bound lipase from a wild-type aspergillus oryzae strain.a mycelium-bound lipase from aspergillus oryzae (aol) exhibited excellent enantioselectivity for kinetic resolution of (rs)-1-phenylethanol in organic solvent. the various reaction parameters affecting the conversion and enantioselectivity were studied, including type of acyl donor, solvent, molar ratio, temperature, enzyme amount and substrate concentration. the optimum reaction conditions were found to be transesterification with vinyl acetate at 30°c in methyl tert-butyl ether with a vinyl ac ...201626854002
comparison of catalytic properties of multiple β-glucosidases of trichoderma reesei.ten putative trichoderma reesei β-glucosidase (bgl) isozymes were heterologously expressed in escherichia coli and aspergillus oryzae and purified to homogeneity. catalytic properties of nine enzymes which showed hydrolytic activity on cellobiose and p-nitrophenyl-β-d-glucopyranoside (pnpg) were investigated. three bgls, encoded by the genes cel3a, cel3b, and cel3e, contained a predicted signal peptide, showed higher hydrolytic activity on cello-oligosaccharides than on pnpg, and preferred longe ...201626846743
antiviral and cytotoxic isocoumarin derivatives from an endophytic fungus aspergillus oryzae.oryzaeins a-d (1-4), four new isocoumarin derivatives, along with five known ones (5-9) were isolated from solid cultures of an endophytic fungus aspergillus oryzae. their structures were elucidated by detailed spectroscopic analysis and by comparison with reported data of related derivatives. among them, compounds 1 and 2 represent the first examples of isocoumarins possessing an unusual 2-oxopropyl group and a rare 3-hydroxypropyl group. compounds 1 and 2 displayed moderate anti-tobacco mosaic ...201626824624
anti-obesity effect of extract from fermented curcuma longa l. through regulation of adipogenesis and lipolysis pathway in high-fat diet-induced obese rats.even though curcuma longa l. possesses various biological activities, it has strong flavor and taste, which decrease consumer palatability and limit industrial applications in food.201626822962
optimization of liquid-state fermentation conditions for the glyphosate degradation enzyme production of strain aspergillus oryzae by ultraviolet mutagenesis.this study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. spore from aspergillus oryzae a-f02 was subjected to ultraviolet mutagenesis. single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. four mutant strains were obtained and named as fujx 001, fujx 002, fujx 0 ...201626795747
preventive effects of fermented brown rice and rice bran against n-nitrosobis (2-oxopropyl) amine-induced pancreatic tumorigenesis in male hamsters.fermented brown rice by aspergillus oryzae (fbra) is known to have the potential to prevent chemical carcinogenesis of the colon, liver, esophagus, urinary bladder, stomach and lungs in rodents. the present study examined the possible chemopreventive effects of fbra on n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic tumorigenesis in hamsters. five-week-old male syrian golden hamsters were divided into seven groups. groups 1-5 were subcutaneously injected with bop (10 mg/kg body weight) f ...201526788138
enzymatically hydrolysed, acetylated and dually modified corn starch: physico-chemical, rheological and nutritional properties and effects on cake quality.corn starch was treated by enzymatic hydrolysis with aspergillus oryzae s2 α-amylase, acetylation with vinyl acetate, and dual modification. the dual modified starch displayed a higher substitution degree than the acetylated starch and lower reducing sugar content than the hydrolysed starch. the results revealed that the cooling viscosity and amylose content of those products decrease (p < 0.05). an increase in moisture, water, and oil absorption capacity was observed for the acetylated starch a ...201626787967
fermented brown rice extract causes apoptotic death of human acute lymphoblastic leukemia cells via death receptor pathway.mixture of brown rice and rice bran fermented with aspergillus oryzae, designated as fbra, has been reported to reveal anti-carcinogenic and anti-inflammatory effects in rodents. then, to test its potential anti-cancer activity, the aqueous extract was prepared from fbra powder, and the effect of this extract on human acute lymphoblastic leukemia jurkat cells was directly examined. the exposure to fbra extract reduced the cell viability in a concentration- and time-dependent manner. the reductio ...201626769704
identification of the gene encoding isoprimeverose-producing oligoxyloglucan hydrolase in aspergillus oryzae.aspergillus oryzae produces a unique β-glucosidase, isoprimeverose-producing oligoxyloglucan hydrolase (ipase), that recognizes and releases isoprimeverose (α-d-xylopyranose-(1 → 6)-d-glucopyranose) units from the non-reducing ends of oligoxyloglucans. a gene encoding a. oryzae ipase, termed ipea, was identified and expressed in pichia pastoris. with the exception of cellobiose, ipea hydrolyzes a variety of oligoxyloglucans and is a member of the glycoside hydrolase family 3. xylopyranosyl branc ...201626755723
fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study.intraductal papillary mucinous neoplasm (ipmn) is an intraductal mucin-producing pancreatic neoplasm with the potential for malignant transformation. changes in glycans expressed on the cell surface and glycotransferases play important roles in malignant transformation. we conducted this study to analyze glycan alterations in ipmns by using a lectin microarray and to identify the factors associated with altered glycans and their relationships with malignant transformation.201626754572
illuminating the binding interactions of galactonoamidines during the inhibition of β-galactosidase (e. coli).several galactonoamidines were previously identified as very potent competitive inhibitors that exhibit stabilizing hydrophobic interactions of the aglycon in the active site of β-galactosidase (aspergillus oryzae). to elucidate the contributions of the glycon to the overall inhibition ability of the compounds, three glyconoamidine derivatives with alteration in the glycon at c-2 and c-4 were synthesized and evaluated herein. all amidines are competitive inhibitors of β-galactosidase (escherichi ...201626740154
production of 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid in the fungus aspergillus oryzae: a step towards heterologous production of pyrethrins in fungi.pyrethrins are natural insecticides, which accumulate to high concentrations in pyrethrum (chrysanthemum cinerariaefolium) flowers. synthetic pyrethroids are more stable, more efficacious and cheaper, but contemporary requirements for safe and environmentally friendly pesticides encourage a return to the use of natural pyrethrins, and this would be favoured by development of an efficient route to their production by microbial fermentation. the biosynthesis of pyrethrins involves ester linkage be ...201626718544
improving 3'-hydroxygenistein production in recombinant pichia pastoris using periodic hydrogen peroxide-shocking strategy.3'-hydroxygenistein can be obtained from the biotransformation of genistein by the engineered pichia pastoris x-33 strain, which harbors a fusion gene composed of cyp57b3 from aspergillus oryzae and a cytochrome p450 oxidoreductase gene (scpr) from saccharomyces cerevisiae. p. pastoris x-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. one mutant (p2-d14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold m ...201626699751
development of a genome editing technique using the crispr/cas9 system in the industrial filamentous fungus aspergillus develop a genome editing method using the crispr/cas9 system in aspergillus oryzae, the industrial filamentous fungus used in japanese traditional fermentation and for the production of enzymes and heterologous proteins.201626687199
metabolic engineering of long chain-polyunsaturated fatty acid biosynthetic pathway in oleaginous fungus for dihomo-gamma linolenic acid production.microbial lipids are promising alternative sources of long chain-polyunsaturated fatty acids (lc-pufas) for food, feed, nutraceutical and pharmaceutical sectors. dihomo-γ-linolenic acid (c20:3δ(8,11,14); dgla) is an important lc-pufas with anti-inflammatory and anti-proliferative effects. to generate a dgla-producing strain, fatty acid reconstitution in aspergillus oryzae was performed by metabolic engineering through co-expression of codon-optimized pythium δ(6)-desaturase and δ(6)-elongase, wh ...201626686314
chitosan crosslinked with genipin as support matrix for application in food process: support characterization and β-d-galactosidase order to develop safer processes for the food industry, we prepared a chitosan support with the naturally occurring crosslinking reagent, genipin, for enzyme. as application model, it was tested for the immobilization of β-d-galactosidase from aspergillus oryzae. chitosan particles were obtained by precipitation followed by adsorption of the enzyme and crosslinking with genipin. the particles were characterized by fourier transform infrared (ftir) spectroscopy and thermogravimetric analysis ( ...201626686119
ethanol and protein from ethanol plant by-products using edible fungi neurospora intermedia and aspergillus oryzae.feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. an alternative is to partially include the process in the first-generation plants. whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. ethanol production and the proteins from the stillage were investigated using ...201526682213
recombinant expression, purification, and characterization of an acyl-coa binding protein from aspergillus characterize biochemically the lipid metabolism-regulating acyl-coa binding protein (acbp) from the industrially-important fungus aspergillus oryzae.201626677124
fermented brown rice and rice bran with aspergillus oryzae (fbra) prevents inflammation-related carcinogenesis in mice, through inhibition of inflammatory cell infiltration.we have established an inflammation-related carcinogenesis model in mouse, in which regressive qr-32 cells subcutaneously co-implanted with a foreign body-gelatin sponge-convert themselves into lethal tumors due to massive infiltration of inflammatory cells into the sponge. animals were fed with a diet containing 5% or 10% fermented brown rice and rice bran with aspergillus oryzae (fbra). in 5% and 10% fbra diet groups, tumor incidences were lower (35% and 20%, respectively) than in the non-trea ...201526670250
evaluation of kojic acid production in a repeated-batch pcs biofilm this study, kojic acid, a secondary metabolite as an industrially important compound, was produced by aspergillus oryzae (a. oryzae), which was immobilized in plastic composite support (pcs) bioreactor. nitrogen deficient medium was applied to increase the production of ka in pcs-immobilized bioreactor. the efficiency of immobilized culture for kojic acid (ka) production and the effect of morphology of a. oryzae on ka production were evaluated. after three cycles of cultivation, 83.47 g/l of ...201626657710
towards effective and stable probiotics.probiotics are live microorganisms, generally either lactobacilli or bifidobacteria, which when administered in adequate amounts confer a health benefit to the host [1]. due to the growing evidence of health benefits associated with their use, probiotics are of increasing interest and represent now a significant growth area in the functional foods industry [2]. however, to be effective, orally administered probiotics should survive preparation of dosage forms and passage through acidic environme ...201526639716
antioxidant activity and anti-inflammatory activity of ethanol extract and fractions of doenjang in lps-stimulated raw 264.7 macrophages.fermentation can increase functional compounds in fermented soybean products, thereby improving antioxidant and/or anti-inflammatory activities. we investigated the changes in the contents of phenolics and isoflavones, antioxidant activity and anti-inflammatory activity of doenjang during fermentation and aging.201526634044
multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design.a simplex-centroid design comprising three solvents (water, ethanol and methanol) was used to optimise the extraction mixture for phenolics and antioxidant activities from defatted soy flour fermented with monascus purpureus or aspergillus oryzae. total phenolics were more efficiently extracted using only water for both samples. the highest antioxidant activities by the dpph and abts methods were obtained using extraction mixtures containing at least 75 wt% water. specific water:ethanol:methanol ...201626616938
novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the japanese sake flavor, ethyl leucate.ethyl-2-hydroxy-4-methylpentanoate (ethyl leucate) contributes to a fruity flavor in japanese sake. the mold aspergillus oryzae synthesizes leucate from leucine and then the yeast saccharomyces cerevisiae produces ethyl leucate from leucate during sake fermentation. here, we investigated the enzyme involved in leucate synthesis by a. oryzae. the a. oryzae gene/cdna encoding the enzyme involved in leucate synthesis was identified and expressed in e. coli and a. oryzae host cells. the purified rec ...201626615399
effect of canavalia gladiata extract fermented with aspergillus oryzae on the development of atopic dermatitis in nc/nga mice.canavalia gladiata has been used as a chinese traditional folk medicine for its anti-inflammatory properties. however, the use of c. gladiata is limited because it contains antinutritional and allergy-causing proteins. we fermented c. gladiata with aspergillus oryzae and investigated the effects of fermented c. gladiata (fcg) on the development of atopic dermatitis (ad) in mice. the mice were divided into five groups: untreated balb/c mice; ad control (nc/nga mice); fcgh (nc/nga mice fed a dieta ...201526613583
heterologous expression and characterization of a thermostable exo-β-d-glucosaminidase from aspergillus exo-β-d-glucosaminidase (aorcsxa) from aspergillus oryzae fl402 was heterologously expressed and purified. the deduced amino acid sequence indicated that aorcsxa belonged to glycoside hydrolase family 2. aorcsxa digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-d-glucosaminidase (csxa) activity. aorcsxa exhibited optimal activity at ph 5.5 and 50°c; however, the enzyme expressed in pichia pastoris (ppaorcsxa) showed much stronger thermostab ...201626597529
lignin degradation in corn stalk by combined method of h2o2 hydrolysis and aspergillus oryzae cgmcc5992 liquid-state fermentation.lignin peroxidase (lip) is the primary enzyme responsible for lignin degradation. in our previous work, in order to shorten the pretreatment time and increase the lignin degradation, we have pretreated the corn stalk (cs) using a combination of aspergillus oryzae cgmcc 5992 solid-state fermentation and h2o2 treatment.201526594239
lactose hydrolysis in milk and dairy whey using microbial β-galactosidases.this work aimed at evaluating the influence of enzyme concentration, temperature, and reaction time in the lactose hydrolysis process in milk, cheese whey, and whey permeate, using two commercial β-galactosidases of microbial origins. we used aspergillus oryzae (at temperatures of 10 and 55°c) and kluyveromyces lactis (at temperatures of 10 and 37°c) β-galactosidases, both in 3, 6, and 9 u/ml concentrations. in the temperature of 10°c, the k. lactis β-galactosidase enzyme is more efficient in th ...201526587283
deciphering the signaling mechanisms of the plant cell wall degradation machinery in aspergillus oryzae.the gene expression and secretion of fungal lignocellulolytic enzymes are tightly controlled at the transcription level using independent mechanisms to respond to distinct inducers from plant biomass. an advanced systems-level understanding of transcriptional regulatory networks is required to rationally engineer filamentous fungi for more efficient bioconversion of different types of biomass.201526573537
[novel type iii polyketide synthase csyb from aspergillus oryzae]. 201526571621
a biosensor based on gold nanoparticles stabilized in poly(allylamine hydrochloride) and decorated with laccase for determination of nanoparticles stabilized in poly(allylamine hydrochloride) (aunp-pah) were used as a support for the immobilization of the enzyme laccase obtained from genetically-modified microorganisms (aspergillus oryzae) and successfully applied in the development of a new biosensor for the determination of dopamine by square-wave voltammetry. the electrochemical characterization of the biosensor was performed by cyclic voltammetry and electrochemical impedance spectroscopy and indicated that the nanom ...201626566574
safety evaluation of amp deaminase from aspergillus oryzae.adenosine-5'-monophosphate (amp) deaminase is an enzyme used to increase concentrations of 5'-inosine monophosphate in certain foods and beverages for flavoring purposes. one commercial source of this enzyme is aspergillus oryzae, a filamentous fungus with a history of safe use in asia as a fermentation organism used in the production of miso sauce and sake liquors. noting the use of the enzyme in food intended for human consumption and potential presence at trace levels in finished goods, a ser ...201526559900
an unusual chimeric diterpene synthase from emericella variecolor and its functional conversion into a sesterterpene synthase by domain swapping.di- and sesterterpene synthases produce c20 and c25 isoprenoid scaffolds from geranylgeranyl pyrophosphate (ggpp) and geranylfarnesyl pyrophosphate (gfpp), respectively. by genome mining of the fungus emericella variecolor, we identified a multitasking chimeric terpene synthase, evvs, which has terpene cyclase (tc) and prenyltransferase (pt) domains. heterologous gene expression in aspergillus oryzae led to the isolation of variediene (1), a novel tricyclic diterpene hydrocarbon. intriguingly, i ...201626546087
reconstitution of biosynthetic machinery of fungal polyketides: unexpected oxidations of biosynthetic intermediates by expression host.reconstitution of whole biosynthetic genes in aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. in the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic g ...201626539980
a long natural-antisense rna is accumulated in the conidia of aspergillus oryzae.analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csra) in aspergillus oryzae. however, the all transcripts were transcribed with opposite direction to the gene csra. the sequence analysis of the transcript revealed that the rna overlapped mrna of csra with 3'-end, and did not code protein longer than 60 amino acid residues. we designated the transcript con ...201626539735
aspergillus associated with meju, a fermented soybean starting material for traditional soy sauce and soybean paste in korea.aspergillus is an important fungal genus used for the fermentation of asian foods; this genus is referred to as koji mold in japan and china. a. oryzae, a. sojae, and a. tamari are used in the production of miso and shoyu in japan, but a comprehensive taxonomic study of aspergillus isolated from meju, a fermented soybean starting material for traditional soy sauce and soybean paste in korea, has not been conducted. in this study, various aspergillus species were isolated during a study of the my ...201526539037
harnessing biodiesel-producing microbes: from genetic engineering of lipase to metabolic engineering of fatty acid biosynthetic pathway.microbial production routes, notably whole-cell lipase-mediated biotransformation and fatty-acids-derived biosynthesis, offer new opportunities for synthesizing biodiesel. they compare favorably to immobilized lipase and chemically catalyzed processes. genetically modified whole-cell lipase-mediated in vitro route, together with in vivo and ex vivo microbial biosynthesis routes, constitutes emerging and rapidly developing research areas for effective production of biodiesel. this review presents ...201726526353
toward rational thermostabilization of aspergillus oryzae cutinase: insights into catalytic and structural stability.cutinases are powerful hydrolases that can cleave ester bonds of polyesters such as poly(ethylene terephthalate) (pet), opening up new options for enzymatic routes for polymer recycling and surface modification reactions. cutinase from aspergillus oryzae (aoc) is promising owing to the presence of an extended groove near the catalytic triad which is important for the orientation of polymeric chains. however, the catalytic efficiency of aoc on rigid polymers like pet is limited by its low thermos ...201626522152
stereoselective behavior of the chiral herbicides diclofop-methyl and diclofop during the soy sauce brewing process.chiral pesticides are now receiving more and more attention in the food-making process. this experiment studied the enantioselective behavior of diclofop-methyl (dm) and its main metabolite, diclofop (dc), during the soy sauce brewing process. two kinds of commonly used strains, aspergillus oryzae and saccharomyces rouxii, were investigated. however, they showed a different degradation ability to the enantiomers of dm and dc. it was observed that (-)-(s)-dm was degraded much faster than (+)-(r)- ...201626501920
modeling and verification of process parameters for the production of tannase by aspergillus oryzae under submerged fermentation using agro-wastes.tannase production by aspergillus oryzae using various agro-wastes as substrates by submerged fermentation was studied in this research. the microbe was isolated from degrading corn kernel obtained from the corn fields at tiruchengode, india. the microbial identification was done using 18s rrna gene analysis. the agro-wastes chosen for the study were pomegranate rind, cassia auriculata flower, black gram husk, and tea dust. the process parameters chosen for optimization study were substrate conc ...201726498327
unfolded protein response is required for aspergillus oryzae growth under conditions inducing secretory hydrolytic enzyme production.unfolded protein response (upr) is an intracellular signaling pathway for adaptation to endoplasmic reticulum (er) stress. in yeast upr, ire1 cleaves the unconventional intron of hac1 mrna, and the functional hac1 protein translated from the spliced hac1 mrna induces the expression of er chaperone genes and er-associated degradation genes for the refolding or degradation of unfolded proteins. in this study, we constructed an irea (ire1 ortholog) conditionally expressing strain of aspergillus ory ...201526496881
aorim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus aspergillus oryzae.the serine-threonine kinase rim15p is a master regulator of stress signaling and is required for stress tolerance and sexual sporulation in the yeast saccharomyces cerevisiae. however, in filamentous fungi that reproduce asexually via conidiation, the physiological function of rim15p homologs has not been extensively analyzed. here, we functionally characterized the protein homolog of rim15p in the filamentous fungus aspergillus oryzae, by deleting and overexpressing the corresponding aorim15 ge ...201626467693
modification of prenylated stilbenoids in peanut (arachis hypogaea) seedlings by the same fungi that elicited them: the fungus strikes back.aspergillus oryzae and rhizopus oryzae were compared for inducing the production of prenylated stilbenoids in peanut seedlings. the fungus was applied at two different time points: directly after soaking (day 1) or after 2 days of germination (day 3). aspergillus- and rhizopus-elicited peanut seedlings accumulated an array of prenylated stilbenoids, with overlap in compounds induced, but also with compounds specific to the fungal treatment. the differences were confirmed to be due to modificatio ...201526458982
production of the natural iron chelator deferriferrichrysin from aspergillus oryzae and evaluation as a novel food-grade antioxidant.deferriferrichrysin (dfcy) is a siderophore found in foods fermented by aspergillus oryzae and is a promising candidate for an antioxidant food additive because of its high binding constant toward iron. however, the dfcy concentration is typically low in foods and cultures.201626399367
purification and enzymatic characterization of a novel β-1,6-glucosidase from aspergillus this study, among the 10 genes that encode putative β-glucosidases in the glycoside hydrolase family 3 (gh3) with a signal peptide in the aspergillus oryzae genome, we found a novel gene (ao090038000425) encoding β-1,6-glucosidase with a substrate specificity for gentiobiose. the transformant harboring ao090038000425, which we named bglh, was overexpressed under the control of the improved glaa gene promoter to form a small clear zone around the colony in a plate assay using 4-methylumbellife ...201626320404
effect of different soybean meal type on ileal digestibility of amino acid in weaning experiment was conducted to evaluate apparent (aid) and standardized (sid) ileal digestibilities of crude protein (cp) and amino acids (aa) with 6 soybean products in weaning pigs. a total of 14 weaning barrows with an initial body weight of 6.54 ± 0.34 kg were fitted with t-cannula at the distal ileum and allotted to 7 diets containing various soybean products. the soybean products used in the experiment were conventional soybean meal (csbm), sbm fermented by aspergillus oryzae gb-107 (fsbma ...201526290731
inhibition of melanogenesis by yeast extracts from cultivations of recombinant pichia pastoris catalyzing ortho-hydroxylation of flavonoids.the inhibition of melanogenesis by yeast extracts from cultivations of recombinant pichia pastoris catalyzing ortho-hydroxylation of flavonoids was investigated. the recombinant yeast harbored a fusion gene composed of the cyp57b3 gene from aspergillus oryzae and a cytochrome reductase gene from saccharomyces cerevisiae. ten flavonoids belonging to flavones, flavonols, flavanones, flavanols, and isoflavones were evaluated for biotransformation by the recombinant strain. the results showed that f ...201526278529
soluble extract of soybean fermented with aspergillus oryzae gb107 inhibits fat accumulation in cultured 3t3-l1 adipocytes.this study was conducted to investigate the effects of fermented soybean (fs) extract on adipocyte differentiation and fat accumulation using cultured 3t3-l1 adipocytes.201526244085
cyclopiazonic acid biosynthesis gene cluster gene cpam is required for speradine a biosynthesis.speradine a is a derivative of cyclopiazonic acid (cpa) found in culture of an aspergillus tamarii isolate. heterologous expression of a predicted methyltransferase gene, cpam, in the cpa biosynthesis gene cluster of a. tamarii resulted in the speradine a production in a 2-oxocpa producing a. oryzae strain, indicating cpam is involved in the speradine a biosynthesis.201526207447
a new electrochemical substrate for rapid and sensitive in vivo monitoring of β-galactosidase gene expressions.a 4-methoxyphenyl-β-galactopyranoside (4-mpgal) substrate incorporating 4-methoxy phenol (4-mp) as an electrochemical reporter is described for the monitoring of β-galactosidase (β-gal) gene expressions. β-gal derived from escherichia coli (e. coli) and aspergillus oryzae (a. oryzae) were investigated, while a graphene oxide film modified electrode was employed as the transducer. the electrochemical signal of 4-mpg within 4-mpgal was masked by protecting their hydroxyl group with galactose. the ...201526192109
effect of novel starter culture on reduction of biogenic amines, quality improvement, and sensory properties of doenjang, a traditional korean soybean fermented sauce select appropriate microorganisms as starter cultures for the reliable and reproducible fermentation of soybean fermented products of korean doenjang, various ratios of fungi (aspergillus oryzae j, mucor racemosus 15, m. racemosus 42) combined with bacillus subtilis tksp 24 were selected as either single, double, or multiple meju strains for commercial mass production of doenjang, followed by analysis of sensory characteristics. in the sensory evaluation, doenjang bam15-1 and bam42-1, which w ...201526147854
growth, ruminal measurements, and health characteristics of holstein bull calves fed an aspergillus oryzae fermentation extract.a fermentation extract of the fungus aspergillus oryzae can be used as a prebiotic. the objective was to determine if dietary inclusion of a fermentation extract of a. oryzae as well as calf age would alter growth, health, performance parameters, and the growth and development of the rumen in holstein calves from birth thru 1 wk postweaning; it was hypothesized that it would. purchased bull calves (n=52) that originated from 1 of 13 farms were used in this experiment. all calves had serum igg gr ...201526142841
occupational sensitization to lactase in the dietary supplement industry.aerogen lactase exposure carries a risk for the development of allergic asthma and rhinitis; only a few occupationally affected patients have been reported. the authors report the results of allergy testing with employees of a lactase tablets manufacturing plant. the survey involved 13 workers, including a questionnaire, spirometry, basophil activation test (bat), and skin prick tests (spts) with lactase and a panel of common aeroallergens. furthermore, lactase-specific immunoglobulin e (ige) an ...201626134755
endocytosis of a maltose permease is induced when amylolytic enzyme production is repressed in aspergillus the filamentous fungus aspergillus oryzae, amylolytic enzyme production is induced by the presence of maltose. previously, we identified a putative maltose permease (malp) gene in the maltose-utilizing cluster of a. oryzae. malp disruption causes a significant decrease in α-amylase activity and maltose consumption, indicating that malp is a maltose transporter required for amylolytic enzyme production in a. oryzae. although the expression of amylase genes and malp is repressed by the presence ...201526117687
water-soluble benzodiazepine prodrug/enzyme combinations for intranasal rescue therapies.benzodiazepines (bzds), including diazepam (dzp) and midazolam (mdz), are drugs of choice for rapid treatment of seizure emergencies. current approved use of these drugs involves administration via either intravenous or rectal routes. the former requires trained medical personnel, while the latter is socially unacceptable for many patients and caregivers. in recent years, efforts have been made to formulate bzds for nasal administration. because of the low solubility of these molecules, organic ...201526115606
synthesis and evaluation of galacto-noeurostegine and its 2-deoxy analogue as glycosidase epimer of the known glycosidase inhibitor noeurostegine, galacto-noeurostegine, was synthesised in 21 steps from levoglucosan and found to be a potent, competitive and highly selective galactosidase inhibitor of aspergillus oryzae β-galactosidase. galacto-noeurostegine was not found to be an inhibitor of green coffee bean α-galactosidase, yeast α-glucosidase and e. coli β-galactosidase, whereas potent but non-competitive inhibition against sweet almond β-glucosidase was established. the 2-deo ...201526111992
cooperative role of calnexin and tiga in aspergillus oryzae glycoprotein folding.calnexin (cnx), known as a lectin chaperone located in the endoplasmic reticulum (er), specifically recognizes g1m9gn2-proteins and facilitates their proper folding with the assistance of erp57 in mammalian cells. however, it has been left unidentified how cnx works in aspergillus oryzae, which is a filamentous fungus widely exploited in biotechnology. in this study, we found that a protein disulfide isomerase homolog tiga can bind with a. oryzae cnx (aocnx), which was revealed to specifically r ...201526085184
simultaneous synthesis of mixtures of lactulose and galacto-oligosaccharides and their selective fermentation.lactulose and galacto-oligosaccharides (gos) are well recognized prebiotics derived from lactose. in the synthesis of lactulose with β-galactosidases gos are also produced, but the ratio of lactulose and gos in the product can be tuned at will, depending on the operation conditions, so to obtain an optimal product distribution in terms of prebiotic potential. the selectivity of fermentation of each carbohydrate alone as well as mixtures of both was determined using ph-controlled anaerobic batch ...201526080080
structure activity relationship study of burkholdine analogues toward simple antifungal agents.cyclic and linear lipopeptides, burkholdine analogues, were synthesized by conventional fmoc-spps and cyclisation with dipc/hobt in the solution phase. synthesized peptides were evaluated for antifungal activities with mic values against saccharomyces cerevisiae and aspergillus oryzae. as a result, the stereochemistry of the amino acid residues and sequences of burkholdine analogues exerted a significant influence on antifungal activities. in addition, we found a linear burkholdine analogue with ...201526077490
bioconversion of capsaicin by aspergillus oryzae.this study identified metabolites of capsaicin bioconverted by aspergillus oryzae, which is generally used for mass production of gochujang prepared by fermenting red pepper powder in korea. a. oryzae was incubated with capsaicin in potato dextrose broth. capsaicin decreased depending on the incubation period, but new metabolites increased. five capsaicin metabolites purified from the ethyl acetate fraction of the capsaicin culture were identified as n-vanillylcarbamoylbutyric acid, n-vanillyl-9 ...201526072923
functional analysis of aoatg11 in selective autophagy in the filamentous fungus aspergillus oryzae.autophagy is a highly conserved cellular degradation process in eukaryotes and consists of both non-selective and selective types. selective autophagic processes include pexophagy, mitophagy, and the cytoplasm-to-vacuole targeting (cvt) pathway of yeast, in which particular vacuolar proteins, such as aminopeptidase i (ape1), are selectively transported to vacuoles. although selective autophagy has been mainly studied in the yeasts saccharomyces cerevisiae and pichia pastoris, there is evidence f ...201526058532
identification and enzymatic characterization of an endo-1,3-β-glucanase from euglena gracilis.euglena produces paramylon as a storage polysaccharide, and is thought to require β-1,3-glucan degrading enzymes to release and utilize the accumulated carbohydrate. to investigate β-1,3-glucan degradation in euglena, endo-1,3-β-glucanases were partially purified from euglena gracilis by hydrophobic, gel filtration and anion-exchange chromatography. tryptic digests and mass-spectrometric analysis identified three proteins in the purified fraction as a member of glycoside hydrolase family (gh) 17 ...201526028521
characterization of two acetyltransferase genes in the pyripyropene biosynthetic gene cluster from penicillium coprobium.pyripyropenes potently and selectively inhibit acyl-coa:cholesterol acyltransferase 2 (acat-2). among multiple isomers of pyripyropene (a to r), pyripyropene a (pya) has insecticidal properties in addition to its growth inhibition properties against human umbilical vein endothelial cells. based on the predicted biosynthetic gene cluster of pyripyropene a, two genes (ppb8 and ppb9) encoding two acetyltransferases (ats) were separately isolated and introduced into the model fungus aspergillus oryz ...201426019565
thermostabilization of aspergillus oryzae β-d-galactosidase.the thermal stability of the aspergillus oryzae β-d-galactosidase (β-gal) was evaluated at 60 °c. the stability of β-gal was dependent on the ph, buffer, and additives. the β-gal exhibited its highest thermal stability in a 0.02 m phosphate buffer ph 6. among all the tested additives, galactose, a competitive inhibitor of β-gal, was the upmost thermal stabilizer. a 0.15 m galactose solution caused β-gal to retain a whole of 98.65% of its initial activity after incubation at 60 °c for 2 h. the se ...201626011544
flavourzyme, an enzyme preparation with industrial relevance: automated nine-step purification and partial characterization of eight enzymes.flavourzyme is sold as a peptidase preparation from aspergillus oryzae. the enzyme preparation is widely and diversely used for protein hydrolysis in industrial and research applications. however, detailed information about the composition of this mixture is still missing due to the complexity. the present study identified eight key enzymes by mass spectrometry and partially by activity staining on native polyacrylamide gels or gel zymography. the eight enzymes identified were two aminopeptidase ...201525996918
biochemical evaluation of xylanases from various filamentous fungi and their application for the deinking of ozone treated newspaper pulp.filamentous fungi, aspergillus oryzae mdu-4 was biochemically selected among different species of aspergillus and trichoderma, for xylanase production. the enzyme activity and specific activity of partially purified xylanase from a. oryzae mdu-4 was 7452 iu/ml and 13,549 iu/g, respectively. temperature and ph optima for xylanase were found to be 60°c and 6.0, respectively. the reaction kinetics of xylanase was found to be km (3.33 mg/ml) and vmax (18,182 μmol/mg). the implementation of ozone tre ...201525965456
effects of dietary cellulase and xylanase addition on digestion, rumen fermentation and methane emission in growing goats.the objective of this study was to evaluate the effectiveness of supplementation of cellulase and xylanase to diets of growing goats to improve nutrient digestibility, utilisation of energy and mitigation of enteric methane emissions. the experiment was conducted in a 5 × 5 latin square design using five goats with permanent rumen fistulae and five treatments consisted of two levels of cellulase crossed over with two levels of xylanase plus unsupplemented control. the cellulase (243 u/g) derived ...201525963843
a multi-criteria analysis approach for ranking and selection of microorganisms for the production of oils for biodiesel production.oleaginous microorganisms have potential to be used to produce oils as alternative feedstock for biodiesel production. microalgae (chlorella protothecoides and chlorella zofingiensis), yeasts (cryptococcus albidus and rhodotorula mucilaginosa), and fungi (aspergillus oryzae and mucor plumbeus) were investigated for their ability to produce oil from glucose, xylose and glycerol. multi-criteria analysis (mca) using analytic hierarchy process (ahp) and preference ranking organization method for the ...201525958151
transcriptome and proteome expression analysis of the metabolism of amino acids by the fungus aspergillus oryzae in fermented soy sauce.amino acids comprise the majority of the flavor compounds in soy sauce. a portion of these amino acids are formed from the biosynthesis and metabolism of the fungus aspergillus oryzae; however, the metabolic pathways leading to the formation of these amino acids in a. oryzae remain largely unknown. we sequenced the transcriptomes of a. oryzae 100-8 and a. oryzae 3.042 under similar soy sauce fermentation conditions. 2d gel electrophoresis was also used to find some differences in protein express ...201525945335
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