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identification of a major xylanase from aspergillus flavus as a 14-kd protein.aspergillus flavus k49 secreted at least two xylanase activities when grown on a medium containing larch (wood) xylan as a sole carbon source. enzyme activity was assayed using an agar medium containing remazol brilliant blue r conjugated oat spelt xylan as substrate. crude enzyme preparations were inhibited by hg(+2), with an ed(50) of 17.5 mm and maximum inhibition of 83% at 50 mm. a concentrated sample of a. flavus k49 xylanase preparation was subjected to gel filtration chromatography on a p ...201121479830
fermentation temperature affects the antioxidant activity of the enzyme-ripened sufu, an oriental traditional fermented product of soybean.in this study, sufu, a chinese traditional fermented product of soybean, was prepared by ripening salted tofu cubes in the mash of aspergillus oryzae-fermented rice-soybean koji possessing various hydrolytic enzymes at 25°c, 37°c and 45°c. antioxidant activity including 2,2-diphenyl-2-picylhydoxyl (dpph) radical-scavenging activity, fe(2+)-chelating ability and reducing power exerted by the methanol extract of sufu was determined and compared with that of the non-fermented tofu extract. it was f ...201121497549
crystallization and diffraction analysis of β-n-acetylhexosaminidase from aspergillus oryzae.fungal β-n-acetylhexosaminidases are enzymes that are used in the chemoenzymatic synthesis of biologically interesting oligosaccharides. the enzyme from aspergillus oryzae was produced and purified from its natural source and crystallized using the hanging-drop vapour-diffusion method. diffraction data from two crystal forms (primitive monoclinic and primitive tetragonal) were collected to resolutions of 3.2 and 2.4 å, respectively. electrophoretic and quantitative n-terminal protein-sequencing ...201121505251
functional analysis of the putative aaa atpase aipa localizing at the endocytic sites in the filamentous fungus aspergillus oryzae.we searched for novel components involved in aspergillus oryzae endocytosis by yeast two-hybrid (yth) screening. using the endocytic marker protein aoabp1 (a. oryzae homolog of saccharomyces cerevisiae abp1p) as bait, a putative aaa (atpases associated with diverse cellular activities) atpase encoded by a gene termed aipa (aoabp1 interacting protein) was identified. further yth analyses showed that the 346-370 amino-acid region of aipa interacts with the sh3 (src homology 3) domains of aoabp1. m ...201121507053
development of an aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production.an aspergillus oryzae whole-cell biocatalyst which coexpresses fusarium heterosporum lipase (fhl) and mono- and di-acylglycerol lipase b (mdlb) in the same cell has been developed to improve biodiesel production. by screening a number of transformants, the best strain was obtained when fhl gene was integrated into a. oryzae chromosome using sc selection marker while mdlb was integrated using niad selection marker. the reaction system using the lipase-coexpressing whole-cells was found to be supe ...201121507622
crystallization and preliminary x-ray analysis of aspergillus oryzae catechol oxidase.catechol oxidase is an enzyme that catalyzes the oxidation of o-diphenols to the corresponding o-quinones. it is a copper-containing enzyme with a binuclear copper active site. here, the crystallization and multiple-wavelength anomalous dispersion data collection of catechol oxidase from the mould fungus aspergillus oryzae are described. during the purification, three forms of the enzyme (39.3, 40.5 and 44.3 kda) were obtained. a mixture of these three forms was initially crystallized and gave c ...201121636908
effect of meat washing on the development of impact odorants in fish miso prepared from spotted mackerel.miso, a fermented soybean paste prepared using koji (rice malt inoculated with aspergillus oryzae) has been commonly used as a traditional seasoning for several centuries in east asian countries. a miso-like fermented product was prepared using washed and unwashed meats of spotted mackerel (scomber australasicus) with improved food functionality and aroma attributes. the evolution of aroma-active volatiles was further evaluated during the early stages of maturation.201121384352
enzymatic properties of the recombinant serine-type carboxypeptidase ocpc, which is unique to aspergillus oryzae.gene ao090103000153 is unique to aspergillus oryzae rib40 and a. flavus nrrl3357, and is speculated to encode a serine-type carboxypeptidase. in this study, we purified and characterized a heterologously expressed gene product of ao090103000153. 5'-rapid amplification of cdna ends indicated that the translation start site of the gene is located 1,586 bp downstream of the translation start site predicted by the genome sequencing project. the gene, starting from the revised translation start codon ...201121512241
kojic acid biosynthesis in aspergillus oryzae is regulated by a zn(ii)(2)cys(6) transcriptional activator and induced by kojic acid at the transcriptional level.a gene encoding the zn(ii)(2)cys(6) transcriptional factor is clustered with two genes involved in biosynthesis of a secondary metabolite, kojic acid (ka), in aspergillus oryzae. we determined that the gene was essential for ka production and the transcriptional activation of ka biosynthetic genes, which were triggered by the addition of ka.201121514215
the influence of douchi starter cultures on the composition of extractive components, microbiological activity, and sensory properties of fermented fish pastes.three experiments were conducted to test the hypothesis that douchi cultures could serve as a potential starter for enhancing the quality attributes of fermented silver carp meat. in experiment 1, an active, prefermented douchi culture was incorporated into a fish paste to aid in the fish fermentation (30 d) and facilitate biochemical production of extractive flavor components (prf). in experiment 2, a fully fermented (30 d) douchi was added to a fish paste and the mixture was fermented for 30 d ...201121535644
ultrasound-assisted generation of ace-inhibitory peptides from casein hydrolyzed with nanoencapsulated protease.background: bioactive peptides generated from milk proteins are eminent ingredients for functional foods and nutraceuticals. amongst several approaches to release these peptides, hydrolysis of milk proteins with proteolytic enzymes is a promising choice. it is, however, required to inactivate the enzyme after a predetermined time, which leads to impurity of the final product. immobilization of enzyme molecules can overcome this problem as it simplifies enzyme separation from the reaction mixture ...201121538371
structure of a dimeric fungal a-type carbonic anhydrase.the crystal structure of aspergillus oryzae carbonic anhydrase (aoca) was determined at 2.7å resolution and it revealed a dimer, which only has precedents in the a class in two membrane and cancer-associated enzymes. a carbonic anhydrases are underrepresented in fungi compared to the ß class, this being the first structural representative. the overall fold and zinc binding site resemble other well studied carbonic anhydrases. a major difference is that the histidine, thought to be the major prot ...201121377464
automatic measurement of sake fermentation kinetics using a multi-channel gas monitor system.in small-scale sake brewing tests, progression of ethanol fermentation is examined by monitoring carbon dioxide emission using the fermograph, an automated multi-channel gas monitor instrument. the fermograph system enables automatic measurements of fermentation profiles with high accuracy, reproducibility, and resolution, and facilitates comprehensive and quantitative sake fermentation kinetic analyses.201121470907
production and characterisation of aosox2 from aspergillus oryzae, a novel flavin-dependent sulfhydryl oxidase with good ph and temperature stability.sulfhydryl oxidases have found application in the improvement of both dairy and baking products due to their ability to oxidise thiol groups in small molecules and cysteine residues in proteins. a genome mining study of the available fungal genomes had previously been performed by our group in order to identify novel sulfhydryl oxidases suitable for industrial applications and a representative enzyme was produced, aosox1 from aspergillus oryzae (faccio et al. bmc biochem 11:31, 2010). as a resul ...201121327412
construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus aspergillus oryzae.a functional library of cytochrome p450 monooxygenases from aspergillus oryzae (aocyps) was constructed in which 121 isoforms were coexpressed with yeast nadph-cytochrome p450 oxidoreductase in saccharomyces cerevisiae. using this functional library, novel catalytic functions of aocyps, such as catalytic potentials of cyp57b3 against genistein, were elucidated for the first time. comprehensive functional screening promises rapid characterization of catalytic potentials and utility of aocyps.201121378053
highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in aspergillus oryzae.in the present study, a system with high lipase expression in aspergillus oryzae was developed using an improved enolase promoter (p-enoa124) and the 5' untranslated region of a heat-shock protein (hsp-utr). p-enoa142 enhanced the transcriptional level of a heterologous lipase gene and hsp-utr improved its translational efficiency. fusarium heterosporum lipase (fhl) was inserted into a psensu-fhl expression vector harboring p-enoa142 and hsp-utr and was transformed into an a. oryzae ns4 strain. ...201121380514
enzyme stabilization and immobilization by sol-gel entrapment.while biocatalysts show tremendous potential for the industrial production of fine chemicals, their integration into large-scale processes has been slow. one of the main reasons for slow acceptance in industry is the inherent instability of the enzymes. recent developments in bioengineering have shed some light on methods of improving enzyme stability. one method that has been used for many decades, successfully to varying degrees, has been the immobilization of enzymes. to this regards, silica ...201120865388
analysis of autophagy in aspergillus oryzae by disruption of aoatg13, aoatg4, and aoatg15 genes.autophagy is a degradation system in which cellular components are digested via vacuoles/lysosomes, and involved in differentiation in addition to helping cells to survive starvation. the autophagic process is composed of several steps: induction of autophagy, formation of autophagosomes, transportation to vacuoles, and degradation of autophagic bodies. to further understand autophagy in the filamentous fungus aspergillus oryzae, we first constructed a. oryzae mutants defective for the aoatg13, ...201121204928
post-translational his-cys cross-linkage formation in tyrosinase induced by copper(ii)-peroxo species.autocatalytic formation of his-cys cross-linkage in the enzyme active site of tyrosinase from aspergillus oryzae has been demonstrated to proceed by the treatment of apoenzyme with cu(ii) under aerobic conditions, where a (μ-η(2):η(2)-peroxo)dicopper(ii) species has been suggested to be involved as a key reactive intermediate.201121218798
five carboxin-resistant mutants exhibited various responses to carboxin and related fungicides.five carboxin-resistant mutants from aspergillus oryzae were characterized by the sensitivities of their mycelial growth and succinate dehydrogenase (sdh) activity to carboxin and three related fungicides. despite a significant resistance to carboxin, exhibited by all the mutants, their patterns of sensitivity to the other fungicides was distinct. this provides clues to the molecular interaction between sdh and these fungicides.201121228470
characterization of an aspergillus oryzae cysteinyl dipeptidase expressed in escherichia coli.cysteinyl dipeptidase from aspergillus oryzae (cdpa) was produced in escherichia coli and purified. the enzyme showed activity specific toward cysteine-containing dipeptides, but its substrate specificity was distinct from those of other cysteinyl dipeptidases of the m20 family. it was optimally active at ph 7-8 and stable at ph 6-9 and at up to 40 °c.201121228467
construction of a thiamine pyrophosphate high-producing strain of aspergillus oryzae by overexpression of three genes involved in thiamine biosynthesis.we have found a gene (thip) encoding thiamine pyrophosphokinase (tpk) in the aspergillus oryzae genome. no riboswitch-like region was found in the upstream region of thip, although it was repressed probably by thiamine pyrophosphate (tpp) as well as thia and nmta, which are strictly regulated by tpp-riboswitch sequence. to improve the productivity of tpp in a. oryzae, we constructed the strain in which thia, nmta and thip were overexpressed simultaneously. the resulting strain accumulated intrac ...201121247799
glutamate production from ß-glucan using endoglucanase-secreting corynebacterium glutamicum.we demonstrate glutamate production from ß-glucan using endoglucanase (eg)-expressing corynebacterium glutamicum. the signal sequence tora derived from escherichia coli k12, which belongs to the tat pathway, was suitable for secreting eg of clostridium thermocellum using c. glutamicum as a host. using the tora signal sequence, endoglucanase from clostridium cellulovorans 743b was successfully expressed, and the secreted eg produced 123 mg of reducing sugar from 5 g of ß-glucan at 30 °c for 72 h, ...201121305281
gene cloning and enzyme structure modeling of the aspergillus oryzae n74 fructosyltransferase.the fructooligosaccharides (fos) represent an important source of prebiotic compounds that are widely used as an ingredient in functional foods. recently, the strain aspergillus oryzae n74 was reported as a potential microorganism for the industrial production of fos, due to its high yields of fos production. in this work, we used a pcr-cloning strategy to clone the a. oryzae n74 ftase gene as a previous step for recombinant enzyme production. ftase showed a 1630 bp size with a 99% similarity wi ...201120563857
functional analysis and subcellular location of two flavohemoglobins from aspergillus oryzae.multiple flavohemoglobin (fhb) homolog genes are found in the genomes of eukaryotic microorganisms, but their functions remain unknown. in this study, two distinct types of fhbs (predictive cytosolic fhb1 and predictive mitochondrial fhb2) from the fungus aspergillus oryzae were investigated to elucidate the physiological roles of these fhbs. the fhb1 gene responded to external nitric oxide (no) stress at the transcriptional level, whereas the fhb2 gene did not. disrupting fhb1 increased cell hy ...201120817113
heterologous expression of aspergillus oryzae xylose reductase and xylitol dehydrogenase genes facilitated xylose utilization in the yeast saccharomyces cerevisiae.the cdna encoding a putative xylose reductase (xyra) from aspergillus oryzae was cloned and coexpressed in the yeast saccharomyces cerevisiae with a. oryzae xylitol dehydrogenase cdna (xdha). xyra exhibited nadph-dependent xylose reductase activity. the s. cerevisiae strain, overexpressing the xyra, xdha, endogenous xks1, and tal1 genes, grew on xylose as sole carbon source, and produced ethanol.201121228482
overexpression and characterization of an extracellular leucine aminopeptidase from aspergillus oryzae.leucine aminopeptidase (lap), an enzyme used in the food industry, is an exopeptidase that removes an amino acid residue, primarily leucine (leu), from the n-terminus of peptides and protein substrates. in this study, we focused on the leucine aminopeptidase a (lapa) gene from aspergillus oryzae rib40. to purify and characterize the lapa, lapa was overexpressed in a. oryzae rib40 using the amyb promoter. lap activity in the culture supernatant of one transformant harboring the lapa expression pl ...201120803144
identification and characterization of a novel fermented substance produced by edible aspergillus oryzae ao-1 that inhibits dpp-iv activity.we identified a novel fermented substance (fka) produced by the edible aspergillus oryzae strain ao-1 that inhibited dipeptidyl peptidase iv (dpp-iv) (ic(50) = 3.41 mg · ml(-1)). hplc analysis of fka showed specific one metabolite (wyk-1), which inhibited dpp-iv (ic(50) = 6.98 μm). wyk-1 was identified as a tetrahydroxyisoquinoline derivative. interestingly, we examined 60 strains of a. oryzae, ao-1 was the only a. oryzae strain that produces wyk-1. this is the first report that an edible a. ory ...201120851672
study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from aspergillus oryzae.the study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. the synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (hplc). according to the reaction of s-6-a catalyzed by fructosyltransferase from aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, ph, substrate and enzyme concentration in the rea ...201120643232
protease inhibitor from moringa oleifera with potential for use as therapeutic drug and as seafood preservative.protease inhibitors are well known to have several applications in medicine and biotechnology. several plant sources are known to return potential protease inhibitors. in this study plants belonging to different families of leguminosae, malvaceae, rutaceae, graminae and moringaceae were screened for the protease inhibitor. among them moringa oleifera, belonging to the family moringaceae, recorded high level of protease inhibitor activity after ammonium sulfate fractionation. m. oleifera, which g ...201123961135
kinetic analysis of batch ethanol acetylation in isothermal non-stationary multiphase systems by lyophilized mycelium of aspergillus oryzae.a relatively complex network of reactions has been investigated, using as a network model the isothermal batch esterification of acetic acid with ethanol in n-heptane catalyzed by lyophilized mycelium of aspergillus oryzae. the kinetic analysis was firstly carried out on the whole system, without any simplification, by means of the well-known integral method. owing to the poor results obtained by this way, we developed an alternative approach, combining initial rates and integral analysis and re ...201124031645
post-genomic approaches to understanding interactions between fungi and their environment.fungi inhabit every natural and anthropogenic environment on earth. they have highly varied life-styles including saprobes (using only dead biomass as a nutrient source), pathogens (feeding on living biomass), and symbionts (co-existing with other organisms). these distinctions are not absolute as many species employ several life styles (e.g. saprobe and opportunistic pathogen, saprobe and mycorrhiza). to efficiently survive in these different and often changing environments, fungi need to be ab ...201122679591
bismuth-inhibitory effects on bacteria and stimulation of fungal growth in vitro.bismuth salicylate was found to inhibit the growth of a range of bacteria and yeast, "candida albicans". in general the growth of bacteria did not result in increase in bismuth solubilisation, in contrast, bismuth solubilisation increased following the growth of c. albicans. a significant increase in the biomass (dry weight) of aspergillus niger and aspergillus oryzae occurred in vitro when these fungi were grown in the presence of bismuth salicylate. biomass increase occurred over a range of bi ...201223961173
the hydrolysis of agro-industrial residues by holocellulose-degrading enzymes.holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. combinations of crude enzymatic extracts from different fungal species, including aspergillus terreus, aspergillus oryzae, aspergillus niger and trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton residue to investigate the hydrolysis of holocellulose structures. a. terreus and a. oryzae were the best ...201224031857
functional analysis of fara transcription factor in the regulation of the genes encoding lipolytic enzymes and hydrophobic surface binding protein for the degradation of biodegradable plastics in aspergillus oryzae.fara is a zn(ii)(2)cys(6) transcription factor which upregulates genes required for growth on fatty acids in filamentous fungi like aspergillus nidulans. fara is also highly similar to the cutinase transcription factor ctf1α of fusarium solani which binds to the cutinase gene promoter in this plant pathogen. this study determines whether fara transcriptional factor also works in the regulation of genes responsible for the production of cutinase for the degradation of a biodegradable plastic, pol ...201222280964
common and distant structural characteristics of feruloyl esterase families from aspergillus oryzae.feruloyl esterases (faes) are important biomass degrading accessory enzymes due to their capability of cleaving the ester links between hemicellulose and pectin to aromatic compounds of lignin, thus enhancing the accessibility of plant tissues to cellulolytic and hemicellulolytic enzymes. faes have gained increased attention in the area of biocatalytic transformations for the synthesis of value added compounds with medicinal and nutritional applications. following the increasing attention on the ...201222745763
improved heterologous protein production by a tripeptidyl peptidase gene (aosedd) disruptant of the filamentous fungus aspergillus oryzae.proteolytic degradation is one of the serious bottlenecks limiting the yields of heterologous protein production by aspergillus oryzae. in this study, we selected a tripeptidyl peptidase gene aosedd (ao090166000084) as a candidate potentially degrading the heterologous protein, and performed localization analysis of the fusion protein aosedd-egfp in a. oryzae. as a result, the aosedd-egfp was observed in the septa and cell walls as well as in the culture medium, suggesting that aosedd is a secre ...201222878738
contribution ratios of amya, amyb, amyc genes to high-level α-amylase expression in aspergillus oryzae.aspergillus oryzae strains express α-amylases abundantly, and the genome reference strain rib40 has three α-amylase genes (amya, amyb, and amyc). however, there is no information on the contribution ratios of individual α-amylase genes to total expression. in this study, we generated single, double, and triple disruptants of α-amylase genes by employing a strain (δligd) with high gene-targeting efficiency and pyrg marker recycling in a. oryzae. all the disruptants showed reduced activities of α- ...201222878189
degradation of terbuthylazine, difenoconazole and pendimethalin pesticides by selected fungi cultures.contamination of waters by xenobiotic compounds such as pesticides presents a serious environmental problem with substantial levels of pesticides now contaminating european water resources. the aim of this work was to evaluate the ability of the fungi fusarium oxysporum, aspergillus oryzae, lentinula edodes, penicillium brevicompactum and lecanicillium saksenae, for the biodegradation of the pesticides terbuthylazine, difenoconazole and pendimethalin in batch liquid cultures. these pesticides ar ...201222878100
isolation and identification of two novel umami and umami-enhancing peptides from peanut hydrolysate by consecutive chromatography and maldi-tof/tof ms.peanut hydrolysate produced by crude protease extract from aspergillus oryzae hn 3.042 was found to elicit intense umami and umami-enhancing effect. taste profiles, amino acid and organic acid composition of peanut hydrolysate and its separation fractions by ultrafiltration were evaluated. the results revealed that peanut hydrolysate was mainly low molecular weight compounds. fractions of 1-3 kda and below 1 kda prominently contributed to the umami taste and umami-enhancing effect of the peanut ...201222868117
metabolomics-based optimal koji fermentation for tyrosinase inhibition supplemented with astragalus radix.the present study was focused on improving the quality of rice koji by fermentation with a selected aspergillus oryzae strain and a plant astragalus radix. a. oryzae kccm 60345 was used as main inoculant and the astragalus radix was added as supplement in rice koji preparation. lc-ms based metabolite analysis and tyrosinase inhibitory activities were studied for different time periods. a. oryzae kccm 60345 fermented rice koji supplemented with astragalus showed higher tyrosinase inhibition activ ...201222738950
enzymatic characteristics of a recombinant neutral protease i (rnpi) from aspergillus oryzae expressed in pichia pastoris.a truncated neutral protease i (npi) from aspergillus oryzae 3.042 was expressed in pichia pastoris with a high enzyme yield of 43101 u/ml. its optimum ph was about 8.0, and it was stable in the ph range of 5.0-9.0. its optimum temperature was about 55 °c and retained >90% activity at 50 °c for 120 min. recombinant npi (rnpi) was inhibited by cu(2+) and edta. eight cleavage sites of rnpi in oxidized insulin b-chain were determined by mass spectrometry, and five of them had high hydrophobic amino ...201223136814
the evolution of three types of indoleamine 2,3 dioxygenases in fungi with distinct molecular and biochemical characteristics.indoleamine 2,3-dioxygenase (ido) is a tryptophan-degrading enzyme and known as a mammalian immunosuppressive molecule. in fungi, the primary role of ido is to supply nicotinamide adenine dinucleotide (nad(+)) via the kynurenine pathway. we previously reported that the koji-mold, aspergillus oryzae has two ido genes, idoα and idoβ. in the present study, we found that a. oryzae also has the third ido, idoγ. these three-types of idos are widely distributed among the pezizomycotina fungi, although ...201222564706
cloning and expression of the aspergillus oryzae glucan 1,3-beta-glucosidase a (exga) in pichia pastoris.the glucan 1,3-beta-glucosidase a gene (exga) from aspergillus oryzae and fused to the saccharomyces cerevisiae signal peptide (α-factor) was expressed under the control of either a constitutive (gap) or an inducible (aox1) promoter in pichia pastoris. a 1.4-fold higher extracellular enzyme activity (2 u/ml) was obtained using the aox1 inducible expression system than with the gap constitutive promoter (1.4 u/ml). the purified recombinant exga enzyme, with a yield of 10 mg protein/l culture supe ...201222782265
population-based study reveals new risk-stratification biomarker panel for barrett's esophagus.the risk of progression of barrett's esophagus (be) to esophageal adenocarcinoma (eac) is low and difficult to calculate. accurate tools to determine risk are needed to optimize surveillance and intervention. we assessed the ability of candidate biomarkers to predict which cases of be will progress to eac or high-grade dysplasia and identified those that can be measured in formalin-fixed tissues.201222771507
monitoring of the microbial communities involved in the soy sauce manufacturing process by pcr-denaturing gradient gel electrophoresis.soy sauce is a traditional seasoning produced through the fermentation of soybeans and wheat using microbes. in this study, the microbial communities involved in the soy sauce manufacturing process were analyzed by pcr-denaturing gradient gel electrophoresis (pcr-dgge). the bacterial dgge profile indicated that the bacterial microbes in the koji were weissella cibaria (weissella confusa, weissella kimchii, weissella salipiscis, lactobacillus fermentum, lactobacillus plantarum, lactobacillus iner ...201222475947
bioremediation of direct dyes in simulated textile effluents by a paramorphogenic form of aspergillus oryzae.azo dyes are extensively used for coloring textiles, paper, food, leather, drinks, pharmaceutical products, cosmetics and inks. the textile industry consumes the largest amount of azo dyes, and it is estimated that approximately 10-15% of dyes used for coloring textiles may be lost in waste streams. almost all azo dyes are synthetic and resist biodegradation, however, they can readily be reduced by a number of chemical and biological reducing systems. biological treatment has advantages over phy ...201222466598
overexpressing transcriptional regulator in aspergillus oryzae activates a silent biosynthetic pathway to produce a novel polyketide.fungal genomes carry many gene clusters seemingly capable of natural product biosynthesis, yet most clusters remain silent. this places a major constraint on the conventional approach of cloning these genes in more amenable heterologous host for the natural product biosynthesis. one way to overcome this difficulty is to activate the silent gene clusters within the context of the target fungus. here, we successfully activated a silent polyketide biosynthetic gene cluster in aspergillus oryzae by ...201222447538
antimicrobial activity of saponin-rich fraction from camellia oleifera cake and its effect on cell viability of mouse macrophage raw 264.7.as a by-product of oil production, several million tons of camellia oleifera cake is discarded every year in china. the aim of this study was to evaluate the antimicrobial activity of a saponin-rich fraction isolated from c. oleifera cake and investigate its effect on the cell viability of raw 264.7, a macrophage-like cell line present in almost all mouse tissues.201222430639
a new zn(ii)(2)cys(6)-type transcription factor bglr regulates β-glucosidase expression in trichoderma reesei.bglr (pi: 52368, beta-glucosidaseregulator) was identified as a new transcription factor that up-regulates expression of specific genes encoding β-glucosidases. based on a comparative genomic analysis to verify snps between trichoderma reesei mutant pc-3-7 and its parent kdg-12, 19 were confirmed. one of the snps was found to cause a missense mutation close to the end of the dna-binding region of bglr that turned out to be a zn(ii)(2)cys(6)-type fungal-specific transcription factor. bglr was fou ...201222425594
amperometric glucose biosensor utilizing fad-dependent glucose dehydrogenase immobilized on nanocomposite electrode.amperometric glucose biosensors utilizing commercially available fad-dependent glucose dehydrogenases from two strains of aspergillus species are described. enzymes were immobilized on nanocomposite electrode consisting of multi-walled carbon nanotubes by entrapment between chitosan layers. unlike the common glucose oxidase based biosensor, the presented biosensors appeared to be o(2)-independent. the optimal amount of enzymes, working potential and ph value of working media of the glucose biose ...201222418262
the influence of inoculum composition on selected bioactive and nutritional parameters of grass pea tempeh obtained by mixed-culture fermentation with rhizopus oligosporus and aspergillus oryzae strains.tempeh is a popular indonesian product obtained from legume seeds by solid-state fermentation with rhizopus sp. the aim of this research was to study the effect of simultaneous mixed-culture fermentation of grass pea seeds on selected parameters of products as compared to traditional tempeh. the inoculum contained different ratios of rhizopus oligosporus and aspergillus oryzae spores. the simultaneous fermentation of grass pea seeds with inoculum consisting of 1.2 × 10(6) r. oligosporus and 0.6  ...201222414936
effect of aspergillus oryzae-challenged germination on soybean isoflavone content and antioxidant activity.application of microbial stress to soybean during germination induces the accumulation of phytoalexins, which have many health benefits. in this study, the effects of stress induced by aspergillus oryzae on the phytochemical composition of germinating soybeans were investigated, and their radical scavenging activity was compared with those of ungerminated (us) and germinated (gs) soybeans. additionally, the antioxidant activity of coumestrol, a soybean phytoalexin, against hydrogen peroxide-indu ...201222409158
extract of fermented brown rice induces apoptosis of human colorectal tumor cells by activating mitochondrial pathway.brown rice fermented with aspergillus oryzae, designated as fbra, is a dietary fiber-rich food, and fully appreciated as one of the prebiotics, which are generally considered to be beneficial to the health of the body, because of stimulating the growth and/or the activity of bacteria in the digestive system. to assess the effectiveness of fbra as a functional food, the direct effect of fbra extract on human colorectal tumor cells was examined. the exposure of hct116 cells to fbra extract reduced ...201222389198
biocatalytic conversion of aloeresin a to aloesin.leaf exudates from aloe species, such as the southern african aloe ferox, are used in traditional medicines for both humans and livestock. this includes aloesin, a skin bleaching product that inhibits the synthesis of melanin. aloesin, (a c-glycoside-5-methylchromone) can be released from aloeresin a, an ester of aloesin, through hydrolysis. the objective of the current study was to identify an enzymatic hydrolysis method for converting aloeresin a to aloesin, resulting in increased concentratio ...201222374229
isolation and characterization of the mhea (most highly expressed) gene of aspergillus oryzae.the amino acid sequence of the mhea gene of aspergillus oryzae encodes a putative metallothionein-like protein 1. the size of the mhea transcript was 497 nt and the mhea promoter was induced by glucose, consistent with results of analysis by northern hybridization and with the pdca promoter, respectively.201223115516
hyphal heterogeneity in aspergillus oryzae is the result of dynamic closure of septa by woronin bodies.hyphae of higher fungi are compartmentalized by septa. these septa contain a central pore that allows for inter-compartmental and inter-hyphal cytoplasmic streaming. the cytoplasm within the mycelium is therefore considered to be a continuous system. in this study, however, we demonstrate by laser dissection that 40% of the apical septa of exploring hyphae of aspergillus oryzae are closed. closure of septa correlated with the presence of a peroxisome-derived organelle, known as woronin body, nea ...201223106143
identification of a gene involved in the synthesis of a dipeptidyl peptidase iv inhibitor in aspergillus oryzae.wyk-1 is a dipeptidyl peptidase iv inhibitor produced by aspergillus oryzae strain ao-1. because wyk-1 is an isoquinoline derivative consisting of three l-amino acids, we hypothesized that a nonribosomal peptide synthetase was involved in its biosynthesis. we identified 28 nonribosomal peptide synthetase genes in the sequenced genome of a. oryzae rib40. these genes were also identified in ao-1. among them, ao090001000009 (wykn) was specifically expressed under wyk-1-producing conditions in ao-1. ...201222843525
removal of off-flavors from sea tangle (laminaria japonica) extract by fermentation with aspergillus oryzae.this study was conducted to reduce off-flavors from sea tangle (laminaria japonica) extract since the typical odor of seaweed is an impediment to consumption of seaweed products. fifty-six odor compounds emitted from the extract were identified using a thermal desorbor and gas chromatography-mass spectrometry. on the basis of expected odor intensity (eoi), isovaleric acid (41.2%), allyl isothiocyanate (22.6%), octanal (11.1%) and acetaldehyde (10.6%) were responsible for about 85.5% of total odo ...201222840201
comparison of expression and enzymatic properties of aspergillus oryzae lysine aminopeptidases apsa and apsb.the apsa and apsb genes encoding family m1 aminopeptidases were identified in the industrial fungus aspergillus oryzae. the apsb was transcriptionally up-regulated up to 2.5-fold in response to the deprivation of nitrogen or carbon sources in growth media, while up-regulation of apsa was less significant. the encoded proteins were bacterially expressed and purified to characterize their enzymatic properties. apsa and apsb were optimally active at ph 7.0 and 35 °c and stable at ph ranges of 6-10 ...201222806190
solid-state fermentation of soybean and corn processing coproducts for potential feed improvement.two agro-industrial coproducts, soybean cotyledon fiber and distiller's dried grains with solubles (ddgs), were used as substrates to evaluate the effect of coculturing three different fungi, aspergillus oryzae, trichoderma reesei, and phanerochaete chrysosporium, on enzyme production by solid-state fermentation (ssf). when soybean fiber was used as the substrate, a maximum xylanase activity of 757.4 iu/g and a cellulase activity of 3.2 iu/g were achieved with the inoculation and incubation of t ...201222799754
a toolkit for heterologous expression of metabolic pathways in aspergillus oryzae.much has been learned about the activities of the key enzymes involved in eukaryotic natural product synthesis by isolating the relevant genes and expressing them in a suitable foreign host. aspergillus oryzae has proved to be an amenable host for the functional analysis of megasynthases from other fungi, but secondary metabolites are often the products of suites of enzymes, and understanding their biosynthesis requires simultaneous expression of several genes. this chapter describes the develop ...201223084942
reconstitution of a secondary metabolite biosynthetic pathway in a heterologous fungal host.expression of multiple genes involved in a particular metabolic pathway in a heterologous host facilitates the study of fungal secondary metabolite biosynthesis and production of useful compounds. two plasmids with different selection markers, argb and the pyrithiamine resistance marker, are used to transform aspergillus oryzae allowing for expression of up to three genes simultaneously.201223065616
manr, a novel zn(ii)2cys6 transcriptional activator, controls the β-mannan utilization system in aspergillus oryzae.fungal endo-β-mannanases (β-mannanases) are widely used as industrial enzymes; however, no transcriptional regulator of β-mannanases has been identified in fungi or other eukaryotic cells to date. to identify a transcriptional regulator of β-mannanases in aspergillus oryzae, a gene-disruptant library of transcriptional regulators was screened for mutants exhibiting reduced β-mannanase activity by using konjac glucomannan as the substrate, and manr, a zn(ii)(2)cys(6) type dna binding protein was ...201223063954
the evolutionary imprint of domestication on genome variation and function of the filamentous fungus aspergillus oryzae.the domestication of animals, plants, and microbes fundamentally transformed the lifestyle and demography of the human species [1]. although the genetic and functional underpinnings of animal and plant domestication are well understood, little is known about microbe domestication [2-6]. here, we systematically examined genome-wide sequence and functional variation between the domesticated fungus aspergillus oryzae, whose saccharification abilities humans have harnessed for thousands of years to ...201222795693
a novel glycosylphosphatidylinositol-anchored glycoside hydrolase from ustilago esculenta functions in β-1,3-glucan degradation.a glycoside hydrolase responsible for laminarin degradation was partially purified to homogeneity from a ustilago esculenta culture filtrate by weak-cation-exchange, strong-cation-exchange, and size-exclusion chromatography. three proteins in enzymatically active fractions were digested with chymotrypsin followed by liquid chromatography-tandem mass spectrometry (lc/ms/ms) analysis, resulting in the identification of three peptide sequences that shared significant similarity to a putative β-1,3- ...201222685137
meju, unsalted soybeans fermented with bacillus subtilis and aspergilus oryzae, potentiates insulinotropic actions and improves hepatic insulin sensitivity in diabetic rats. 201222550941
two new steroidal saponins from the biotransformation product of the rhizomes of dioscorea nipponica.two new steroidal saponins, dioscins e (1) and f (2), along with nine known steroidal saponins, were isolated from the biotransformation product of the rhizomes of dioscorea nipponica using aspergillus oryzae. the structures of new compounds were established as 25(r)-spirost-5-en-21β-methyl-3β-ol-3-o-α-l-rhamnopyranosyl-(1 → 4)-β-d-glucopyranoside (1) and (25r)-spirost-5-en-3β-ol-7-one 3-o-α-l-rhamnopyranosyl-(1 → 4)-β-d-glucopyranoside (2) by detailed spectroscopic analyses including 1d and 2d ...201222582713
serine-type carboxypeptidase kexa of aspergillus oryzae has broader substrate specificity than saccharomyces cerevisiae kex1 and is required for normal hyphal growth and conidiation.aspergillus oryzae has an ortholog of saccharomyces cerevisiae kex1, termed kexa. a truncated form of kexa protein showed serine-type carboxypeptidase activity and somewhat broader substrate specificity than kex1 protease. furthermore, our results indicated that kexa is required for normal growth of a. oryzae and that it might be involved in hyphal branching.201222961905
production of polygalacturonases by aspergillus oryzae in stirred tank and internal- and external-loop airlift reactors.the production of endo- and exo-polygalacturonase (pg) by aspergillus oryzae was assessed in stirred tank reactors (strs), internal-loop airlift reactors (ilars) and external-loop airlift reactors (elars). for str production, we compared culture media formulated with either pectin (wbe) or partially hydrolyzed pectin. the highest enzyme activities were obtained in medium that contained 50% pectin in hydrolyzed form (wbe5). pg production in the three reactor types was compared for wbe5 and low sa ...201222940313
kinetic analysis, expression pattern, and production of a recombinant fungal protease inhibitor of tasar silkworm antheraea mylitta.antheraea mylitta, a tasar silk-producing insect of saturniidae family, expresses a fungal protease inhibitor named as a. mylitta fungal protease inhibitor-1 (amfpi-1). amfpi-1 inhibits alkaline protease of aspergillus oryzae but its mechanism of action is not known. to understand the mode of inhibition of amfpi-1 against the fungal protease, it was purified from the hemolymph of a. mylitta larvae and inhibitory activity against a. oryzae protease was studied. kinetic analysis of purified amfpi- ...201222935928
draft genome sequence of aspergillus oryzae strain 3.042.aspergillus oryzae is the most important fungus for the traditional fermentation in china and is particularly important in soy sauce fermentation. we report the 36,547,279-bp draft genome sequence of a. oryzae 3.042 and compared it to the published genome sequence of a. oryzae rib40.201222933657
jewelry boxes contaminated by aspergillus oryzae: an occupational health risk?in 2009, 100,000 jewelry boxes, manufactured in china, were delivered to a jewelry manufacturer in besançon, france. all the boxes were contaminated by mold. because the workers refused to handle these jewelry boxes, the company contacted our laboratory to determine how to deal with the problem. three choices were available: (1) decontaminate the boxes, (2) return the boxes to the chinese manufacturer, or (3) destroy the entire shipment. based on microscopic identification, the culture analysis ...201222702230
lect-hepa, a glyco-marker derived from multiple lectins, as a predictor of liver fibrosis in chronic hepatitis c patients.assessment of liver fibrosis in patients with chronic hepatitis c (chc) is critical for predicting disease progression and determining future antiviral therapy. lect-hepa, a new glyco-marker derived from fibrosis-related glyco-alteration of serum alpha 1-acid glycoprotein, was used to differentiate cirrhosis from chronic hepatitis in a single-center study. herein, we aimed to validate this new glyco-marker for estimating liver fibrosis in a multicenter study. overall, 183 chc patients were recru ...201222535703
transcripts of a heterologous gene encoding mite allergen der f 7 are stabilized by codon optimization in aspergillus oryzae.we have previously demonstrated that transcripts of an at-biased heterologous gene encoding mite allergen der f 7 from dermatophagoides farinae were polyadenylated prematurely within the coding region when native cdna was expressed in aspergillus oryzae, and that this premature polyadenylation was prevented by the codon optimization of the der f 7 gene, resulting in increased steady-state mrna levels. in this study, we tested the stability of transcription products derived from expression constr ...201222644529
efficient heterologous expression and secretion in aspergillus oryzae of a llama variable heavy-chain antibody fragment v(hh) against egfr.we have constructed a filamentous fungus aspergillus oryzae that secretes a llama variable heavy-chain antibody fragment (v(hh)) that binds specifically to epidermal growth factor receptor (egfr) in a culture medium. a major improvement in yield was achieved by fusing the v(hh) with a taka-amylase a signal sequence (staa) and a segment of 28 amino acids from the n-terminal region of rhizopus oryzae lipase (n28). the yields of secreted, immunologically active anti-egfr v(hh) reached 73.8 mg/1 in ...201222644525
comparative proteome analysis of aspergillus oryzae 3.042 and a. oryzae 100-8 strains: towards the production of different soy sauce flavors.aspergillus oryzae plays a central role in soybean fermentation, particularly in its contribution to the flavor of soy sauce. we present a comparative assessment of the intracellular differences between wild-type strain 3.042 and mutant strain a100-8, at the proteome level. 522 different protein spots were identified by maldi-tof ms, with 134 spots being confirmed by maldi-tof ms/ms. of these, 451 were differentially expressed proteins (deps). there was at least a two-fold increase for 288 spots ...201222634401
analysis of the functions of recombination-related genes in the generation of large chromosomal deletions by loop-out recombination in aspergillus oryzae.loop-out-type recombination is a type of intrachromosomal recombination followed by the excision of a chromosomal region. the detailed mechanism underlying this recombination and the genes involved in loop-out recombination remain unknown. in the present study, we investigated the functions of ku70, ligd, rad52, rad54, and rdh54 in the construction of large chromosomal deletions via loop-out recombination and the effect of the position of the targeted chromosomal region on the efficiency of loop ...201222286092
anti-obesity effect of a standardised ethanol extract from curcuma longa l. fermented with aspergillus oryzae in ob/ob mice and primary mouse adipocytes.we examined the anti-obesity effect of fermented curcuma longa l. (turmeric) standardised ethanol extract (fte) in the c57bl/6j ob/ob mouse model. mice were fed a chow diet containing fte (0, 200, or 500 mg kg⁻¹ body weight) for 9 weeks.201222278718
effects of koji fermented phenolic compounds on the oxidative stability of fish miso.in the present study, aspergillus oryzae-inoculated koji inhibited lipid oxidation in fermented fish paste rich in polyunsaturated fatty acids following a long fermentation period. the fermentation of koji by a. oryzae liberated several bioactive phenolic compounds, including kojic acid and ferulic acid, which were the most abundant. a linear correlation between several phenolic compounds and their bioactive properties, including their radical-scavenging activity, reducing power, metal-chelating ...201222251370
a heptaketide naphthaldehyde produced by a polyketide synthase from nectria haematococca.bostrycoidin and fusarubin are biologically active fungal polyketides produced by nectria haematococca. this azaanthraquinone and naphthoquinone are thought to be biosynthesized via formation of a c(14) heptaketide aldehyde as a common key intermediate. a blast search against the genome of n. haematococca revealed one candidate gene (nechadraft_101778, nhpks1), which encodes a multi-domain polyketide synthase (pks) with a thiol reductase (tr) domain that would facilitate the reductive release of ...201222633689
gene cloning, purification, and characterization of a novel peptidoglutaminase-asparaginase from aspergillus sojae.glutaminase is an enzyme that catalyzes the hydrolysis of l-glutamine to l-glutamate, and it plays an important role in the production of fermented foods by enhancing the umami taste. by using the genome sequence and expressed sequence tag data available for aspergillus oryzae rib40, we cloned a novel glutaminase gene (asgaha) from aspergillus sojae, which was similar to a previously described gene encoding a salt-tolerant, thermostable glutaminase of cryptococcus nodaensis (cngaha). the structu ...201222610430
changes in selected physical property and enzyme activity of rice and barley koji during fermentation and storage.koji are solid-state fermentation products made by inoculating steamed grains with the spores of fungi, particularly aspergillus spp. this research was undertaken to identify the fermentation and storage conditions optimal for the production and maintenance of selected hydrolytic enzymes, such as α-amlyase and protease, in koji. steamed rice and barley were inoculated with 2 × 10 ¹¹ aspergillus oryzae spores per kilogram of grains and fermented for 118 h in a growth chamber at 28 to 32 °c with c ...201222583119
evaluation of enzyme activity and fiber content of soybean cotyledon fiber and distiller's dried grains with solubles by solid state fermentation.to increase the value of coproducts from corn ethanol fermentation and soybean aqueous processing, distiller's dried grains with solubles (ddgs) and soybean cotyledon fiber were used as the substrates for solid state fermentation (ssf) to improve feed digestibility. aspergillus oryzae, trichoderma reesei, and phanerochaete chrysosporium were chosen as they produce desirable enzymes and are widely used in ssf for feed. the results showed that the cellulase and xylanase activities were significant ...201222528656
kinetic modeling of fructooligosaccharide production using aspergillus oryzae n74.in this study, the kinetic for the bioconversion of sucrose to fructooligosaccharides (fos) by free cells of aspergillus oryzae n74 was modeled. in addition, the effect of immobilized glucose isomerase (igi) on fos production yield was evaluated and considered in the kinetic model. the selected kinetic models were based on a proposed reaction mechanism described by elementary rate equations and modified michaelis-menten kinetic equations. the use of igi allowed to increase the fos production yie ...201222528647
synthesis of β-d-fructofuranosyl-(2→1)-2-acetamido-2-deoxy-α-d-glucopyranoside (n-acetylsucrosamine) using β-fructofuranosidase-containing aspergillus oryzae mycelia as a whole-cell catalyst.using soft granules consisting of celite 535 and dried aspergillus oryzae nbrc100959 mycelia containing β-fructofuranosidase as a whole-cell catalyst, n-acetylsucrosamine [β-d-fructofuranosyl-(2→1)-2-acetamido-2-deoxy-α-d-glucopyranoside] was produced from sucrose and 2-acetamido-2-deoxy-d-glucose by enzymatic transfructosylation. the isolated yield of n-acetylsucrosamine from the reaction mixture was 22.1% (from sucrose). the result of n-terminal amino acid sequence analysis indicated that the ...201222525099
genetic, molecular, and biochemical basis of fungal tropolone biosynthesis.a gene cluster encoding the biosynthesis of the fungal tropolone stipitatic acid was discovered in talaromyces stipitatus (penicillium stipitatum) and investigated by targeted gene knockout. a minimum of three genes are required to form the tropolone nucleus: tropa encodes a nonreducing polyketide synthase which releases 3-methylorcinaldehyde; tropb encodes a fad-dependent monooxygenase which dearomatizes 3-methylorcinaldehyde via hydroxylation at c-3; and tropc encodes a non-heme fe(ii)-depende ...201222508998
modified cre-loxp recombination in aspergillus oryzae by direct introduction of cre recombinase for marker gene rescue.marker rescue is an important molecular technique that enables sequential gene deletions. the cre-loxp recombination system has been used for marker gene rescue in various organisms, including aspergilli. however, this system requires many time-consuming steps, including construction of a cre expression plasmid, introduction of the plasmid, and cre expression in the transformant. to circumvent this laborious process, we investigated a method wherein cre could be directly introduced into aspergil ...201222504800
enhancing the value of nitrogen from rapeseed meal for microbial oil production.rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (fan): commercial enzymes (ces) (2.7 mg g⁻¹ dry meal), liquid state fungal pre-treatment (lsf) using aspergillus oryzae (4.6 mg g⁻¹), liquid state fungal pre-treatment followed by fungal autoly ...201222500902
effect of a novel phytase on growth performance, apparent metabolizable energy, and the availability of minerals and amino acids in a low-phosphorus corn-soybean meal diet for broilers.the addition of microbial phytase to diets for broiler chickens has been shown to improve the availability of phytate p, total p, some other minerals, and amino acids. in this study, the effect of a novel microbial phytase expressed by synthetic genes in aspergillus oryzae on amino acid and mineral availability was assessed. phytase was incorporated (1,000 and 2,000 u/kg) into low-p corn-soybean meal-based diets for broilers. broilers received the experimental diets for 3 wk, and excreta were co ...201222499869
cloning, expression, and characterization of a cellobiose dehydrogenase from thielavia terrestris induced under cellulose growth conditions.the enzyme cellobiose dehydrogenase (cdh) is of considerable interest, not only for its biotechnological applications, but also its potential biological role in lignocellulosic biomass breakdown. the enzyme catalyzes the oxidation of cellobiose and other cellodextrins, utilizing a variety of one- and two-electron acceptors, although the electron acceptor employed in nature is still unknown. in this study we show that a cdh is present in the secretome of the thermophilic ascomycete thielavia terr ...201222484439
vector-initiated transitive rna interference in the filamentous fungus aspergillus oryzae.rna interference (rnai), modulates gene expression via cleavage of double-stranded rna (dsrna) by dicer, producing 21-25 nucleotide silence-inducing rnas (sirnas). in association with argonaute containing complexes, these sirnas target sequence-specific degradation of the homologous single-stranded messenger rna. in the majority of eukaryotes, degradation occurs within the boundaries of the dsrna target. in arabidopsis thaliana and caenorhabditis elegans, gene silencing can also take place trans ...201222366516
secretome of aspergillus oryzae in shaoxing rice wine koji.shaoxing rice wine is the most famous and representative chinese rice wine. aspergillus oryzae su16 is used in the manufacture of koji, the shaoxing rice wine starter culture. in the current study, a comprehensive analysis of the secretome profile of a. oryzae su16 in shaoxing rice wine koji was performed for the first time. the proteomic analysis for the identification of the secretory proteins was done using two-dimensional electrophoresis combined with matrix-assisted laser desorption/ionizat ...201222341915
presence and functionality of mating type genes in the supposedly asexual filamentous fungus aspergillus oryzae.the potential for sexual reproduction in aspergillus oryzae was assessed by investigating the presence and functionality of mat genes. previous genome studies had identified a mat1-1 gene in the reference strain rib40. we now report the existence of a complementary mat1-2 gene and the sequencing of an idiomorphic region from a. oryzae strain ao6. this allowed the development of a pcr diagnostic assay, which detected isolates of the mat1-1 and mat1-2 genotypes among 180 strains assayed, including ...201222327593
comparative chemistry of aspergillus oryzae (rib40) and a. flavus (nrrl 3357).aspergillus oryzae and a. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. in this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of a. oryzae (rib40) and a. flavus (nrrl 3357). ...201224957367
kinetics of improved 1,4-alpha-d-glucan glucohydrolase biosynthesis from a newly isolated aspergillus oryzae iib-6 and parameter significance analysis by 2-factorial design.sixteen different mould cultures viz. aspergillus, alternaria, arthroderma, trichoderma, fusarium, penicillium, rhizopus and chochliobolus were isolated from the soil samples of qatar by serial dilution method. the preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar plates. the isolates were then subjected to secondary screening by submerged fermentation (smf). the 1,4-α-d-glucan glucohydrolase (ggh) activ ...201223961361
the effects of supplementation of a novel bacterial 6-phytase on mineral digestibility and plasma minerals in lactating sows.pigs digest p in plant feedstuffs poorly because pigs do not produce sufficient endogenous phytase to hydrolyze p from phytate (inositol hexaphosphate). supplementation of phytase to diets of piglets and grower-finisher pigs increased digestibility of minerals including p and ca; however, data on phytase efficacy in lactating sows are scarce. therefore, effects of adding a bacterial 6-phytase expressed in a strain of aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerla ...201223365301
optimisation of synthesis of oligosaccharides derived from lactulose (fructosyl-galacto-oligosaccharides) with β-galactosidases of different origin.batch synthesis of fructosyl-galacto-oligosaccharides from lactulose was performed with commercial β-galactosidase preparations from aspergillus oryzae, kluyveromyces lactis and bacillus circulans. the enzyme from a. oryzae produced the highest yield and specific productivity of synthesis, being selected for further studies. optimization of fructosyl-galacto-oligosaccharides synthesis was carried out using response surface methodology, considering temperature and initial sugar concentration as v ...201223497880
the supplementation of low-p diets with microbial 6-phytase expressed in aspergillus oryzae improves p digestibility in sows.two trials were conducted to evaluate a novel microbial 6-phytase expressed in aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerland) in gestating and lactating sows. in the first trial, 24 sows (duroc × landrace; 223 kg bw) were offered, at 16 d of gestation, a low-p control diet (formulated to provide 4.0 g total p/kg; 1.5 g digestible p/kg) supplemented with 0, 500, or 1000 phytase activity (fyt)/kg of phytase. two weeks later, fresh feces were sampled from all sow ...201223365297
cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold aspergillus oryzae.a gene coding for a major extracellular chitosanase was isolated from aspergillus oryzae iam2660. it had a multi-domain structure composed of a signal peptide, a catalytic domain, thr- and pro-rich linkers, and repeated peptides (the r3 domain) from the n-terminus. the r3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent.201222232262
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