Effect of steam explosion and microbial fermentation on cellulose and lignin degradation of corn stover.In order to increase nutrient values of corn stover, effects of steam explosion (2.5MPa, 200s) and Aspergillus oryzae (A. oryzae) fermentation on cellulose and lignin degradation were studied. The results showed the contents of cellulose, hemicellulose and lignin in the exploded corn stover were 8.47%, 50.45% and 36.65% lower than that in the untreated one, respectively (P<0.05). The contents of cellulose and hemicellulose in the exploded and fermented corn stover (EFCS) were decreased by 24.36% ...201222104102
Comparison of covalent immobilization of amylase on polystyrene pellets with pentaethylenehexamine and pentaethylene glycol spacers.a-Amylase from Aspergillus oryzae was covalently immobilized onto polystyrene pellets with pentaethylenehexamine (PS-PEHA-Ald) and pentaethylene glycol (PS-PG-Ald) carrying a terminal aldehyde group. Optimum immobilization occured at pH 8.0 and 25 °C, and at pH 7.0 and 35 °C for PS-PEHA-Ald and PS-PG-Ald, respectively. PS-PEHA-Ald immobilized enzyme retained approximately 75% of the initial activity over 45 days of storage, 70% of the initial activity after nine runs of recycling and displayed t ...201121868216
aspergillus oryzae laea regulates kojic acid synthesis genes.kojic acid synthesis genes regulation was investigated in aspergillus oryzae. our results indicate that kojic acid production was lost in the laea disruption strain, but was recovered in the laea complement strain. real-time pcr also confirmed that expression of kojic acid biosynthesis genes decreased in the laea disruption strain, indicating that these genes are under the control of laea.201121897021
rational domain swaps decipher programming in fungal highly reducing polyketide synthases and resurrect an extinct metabolite.the mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. we conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassianin. expression of the hybrid synthetases in aspergillus oryzae led to the production of reprogrammed compounds in which the changes to the methylation pattern and chain length could be mapped to the ...201121899331
enzymatic properties of the glycine d-alanine aminopeptidase of aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji).the gdaa gene encoding s12 family glycine-d: -alanine aminopeptidase (gdaa) was found in the industrial fungus aspergillus oryzae. gdaa shares 43% amino acid sequence identity with the d: -aminopeptidase of the gram-negative bacterium ochrobactrum anthropi. gdaa purified from an a. oryzae gdaa-overexpressing strain exhibited high d: -stereospecificity and efficiently released n-terminal glycine and d: -alanine of substrates in a highly specific manner. the optimum ph and temperature were 8 to 9 ...201122005737
Functional analysis of the cyclopiazonic acid biosynthesis gene cluster in Aspergillus oryzae RIB 40.The cyclopiazonic acid (CPA) nonproducing strain, Aspergillus oryzae RIB 40, does not biosynthesize cyclo-acetoacetyl-L-tryptophan (cAATrp) due to a truncation in the responsible PKS-NRPS gene. We found that RIB 40 converted cAATrp to 2-oxocyclopiazonic acid, the final product of CPA biosynthesis in A. oryzae. This indicates that the CPA biosynthesis gene cluster, except for the PKS-NRPS gene, is functional in RIB 40.201122056451
[homology modeling and molecular docking of xylitol dehydrogenase from aspergillus oryzae].we investigated the structure model and function of xylitol dehydrogenase from aspergillus oryzae.201122043796
Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.Clin Microbiol Infect ABSTRACT: Accurate species discrimination of filamentous fungi is essential, because some species have specific antifungal susceptibility patterns, and misidentification may result in inappropriate therapy. We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for species identification through direct surface analysis of the fungal culture. By use of culture collection strains representing 55 species of Aspergillus, Fusariu ...201121883662
in silico characterization of alkaline proteases from different species of aspergillus.a total of 49 protein sequences of alkaline proteases retrieved from genbank representing different species of aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. the sequence level homology was obtained among different groups of alkaline protease enzymes, viz alkaline serine protease, oryzin, calpain-like protease, serine protease, subtilisin-like alkaline prot ...201122072140
erratum to: enzymatic properties of the glycine d: -alanine aminopeptidase of aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji). 201122120619
proteolysis in dry fermented sausages: the effect of selected exogenous proteases.the effect of three commercial proteases (pronase e from streptomyces griseus, aspartyl proteinase from aspergillus oryzae and papain) on protein breakdown and the sensory characteristics of dry fermented sausages was investigated. water soluble, non-protein, 5% phosphotungstic acid soluble, 5% sulphosalicylic acid soluble and total volatile basic nitrogen contents increased during fermentation, stabilizing later until the end of ripening (26th day). nitrogen values were always greater in the as ...199722061850
A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae.Our goal in this work was to develop a method to minimize the chromosomes of Aspergillus oryzae, to arrive at a deeper understanding of essential gene functions that will help create more efficient industrially useful strains. In a previous study, we successfully constructed a highly reduced chromosome 7 using multiple large-scale chromosomal deletions (Jin et al. in Mol Genet Genomics 283:1-12, 2010). Here, we have created a further reduced chromosome A. oryzae mutant harboring a reduced chrom ...201122207035
cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold aspergillus oryzae.a gene coding for a major extracellular chitosanase was isolated from aspergillus oryzae iam2660. it had a multi-domain structure composed of a signal peptide, a catalytic domain, thr- and pro-rich linkers, and repeated peptides (the r3 domain) from the n-terminus. the r3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent.201222232262
identification and characterization of useful fungi with α-amylase activity from the korean traditional nuruk.the objective of this study was to find useful fungi with α-amylase activity from the korean traditional nuruk for the quality of traditional korean alcoholic beverage. in this study, 165 samples of traditional nuruk were collected from 170 regions throughout korea and the fungi were isolated to a total of 384 strains. in order to investigate the effect of microflora on nuruk, α-amylase activity, saccharogenic power (sp), starch hydrolysis activity and acid producing activity were evaluated. ten ...201122783116
effects of different fermented soy protein and apparent ileal digestible lysine levels on weaning pigs fed fermented soy protein-amended diets.two experiments were conducted to evaluate the effect of different fermented soybean proteins and the apparent ileal digestible lysine levels on weaning pigs fed fermented soy protein (fsp)-amended diets. in exp. 1, 70 crossed piglets (6.25 ± 0.40 kg) were used in a 5-week trial to evaluate two different fsp. in exp. 2, 20 weaning barrows (6.15 ± 0.45 kg) were used in a metabolism trial to determine the effects of the apparent ileal digestible (1.2, 1.3, 1.4 and 1.5%) lysine levels in weaning pi ...201122574792
simultaneous enhancement of free isoflavone content and antioxidant potential of soybean by fermentation with aspergillus order to determine the fungal strain and fermentation period which produce the highest antioxidant activity of fermented soy product, soybean was fermented with aspergillus oryzae nl 5 isolated from korean traditional meju produced in sunchang yeastopia land in korea, and was subjected to analyzing isoflavone profiles and assaying antioxidant activity. antioxidant potential of the samples was evaluated by ferric reducing antioxidant power assay, 2,2'-azinobis (2-ethylbenzthiazoline-6-sulfonic ...201122417591
gc-ms based metabolomics for rapid simultaneous detection of escherichia coli o157:h7, salmonella typhimurium, salmonella muenchen, and salmonella hartford in ground beef and chicken.a metabolomic-based method for rapid detection of escherichia coli o157:h7, salmonella hartford, salmonella typhimurium, and salmonella muenchen in nonselective media was developed. all pathogenic bacteria were grown in tryptic soy broth (tsb) at 37 °c followed by metabolite quantification at 2-h intervals for 24 h. results were compared with the metabolite profiles similarly obtained with e. coli k12, pseudomonas aeruginosa, staphylococcus aureus, saccharomyces cereviseae, and aspergillus oryza ...201122417363
[influence of aflatoxin on vibrio fischeri luminescence].in the present study, we aim to evaluate the inhibitory effect of aflatoxin on vibrio fischeri luminescence.201122379809
synthesis of galacto-oligosaccharides by β-galactosidase from aspergillus oryzae using partially dissolved and supersaturated solution of lactose.the effect of enzyme to substrate ratio, initial lactose concentration and temperature has been studied for the kinetically controlled reaction of lactose transgalactosylation with aspergillus oryzae β-galactosidase, to produce prebiotic galacto-oligosaccharides (gos). enzyme to substrate ratio had no significant effect on maximum yield and specific productivity. galacto-oligosaccharide syntheses at very high lactose concentrations (40, 50 and 60%, w/w, lactose monohydrate) were evaluated at dif ...201122305174
multifunctions of melb, a fungal tyrosinase from aspergillus oryzae.the pro form of melb tyrosinase from the melb gene of aspergillus oryzae was over-produced from e. coli and formed a homodimer that exhibited the spectral features of met-tyrosinase. in the presence of nh(2)oh (reductant), the proenzyme bound dioxygen to give a stable (μ-η(2):η(2) -peroxo)dicopper(ii) species (oxy form), thus indicating that the pro form tyrosinase can function as an oxygen carrier or storage protein like hemocyanin. the pro form tyrosinase itself showed no catalytic activity to ...201122213164
[effect of n-terminal disulfide bridge on thermostability of family 11 xylanases].a mesophilic xylanase from aspergillus oryzae, abbreviated to aoxyn11a, belongs to glycoside hydrolase family 11. using aoxyn11a as the parent, the thermotolerant hybrid xylanase, we constructed aex11a by substituting its n-terminus with the corresponding region of a hyperthermostable family 11 xylanase, evxyn11(ts). aoxyn11a- and aex11a-encoding genes were expressed in pichia pastoris gs115 separately, and effects of temperatures on expressed products were determined and compared. the optimum t ...201223593868
optimisation of synthesis of oligosaccharides derived from lactulose (fructosyl-galacto-oligosaccharides) with β-galactosidases of different origin.batch synthesis of fructosyl-galacto-oligosaccharides from lactulose was performed with commercial β-galactosidase preparations from aspergillus oryzae, kluyveromyces lactis and bacillus circulans. the enzyme from a. oryzae produced the highest yield and specific productivity of synthesis, being selected for further studies. optimization of fructosyl-galacto-oligosaccharides synthesis was carried out using response surface methodology, considering temperature and initial sugar concentration as v ...201223497880
the effects of supplementation of a novel bacterial 6-phytase on mineral digestibility and plasma minerals in lactating sows.pigs digest p in plant feedstuffs poorly because pigs do not produce sufficient endogenous phytase to hydrolyze p from phytate (inositol hexaphosphate). supplementation of phytase to diets of piglets and grower-finisher pigs increased digestibility of minerals including p and ca; however, data on phytase efficacy in lactating sows are scarce. therefore, effects of adding a bacterial 6-phytase expressed in a strain of aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerla ...201223365301
the supplementation of low-p diets with microbial 6-phytase expressed in aspergillus oryzae improves p digestibility in sows.two trials were conducted to evaluate a novel microbial 6-phytase expressed in aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerland) in gestating and lactating sows. in the first trial, 24 sows (duroc × landrace; 223 kg bw) were offered, at 16 d of gestation, a low-p control diet (formulated to provide 4.0 g total p/kg; 1.5 g digestible p/kg) supplemented with 0, 500, or 1000 phytase activity (fyt)/kg of phytase. two weeks later, fresh feces were sampled from all sow ...201223365297
the supplementation of low-p diets with microbial 6-phytase expressed in aspergillus oryzae increases p and ca digestibility in growing pigs.a trial was conducted to evaluate the dose response of a novel microbial 6-phytase expressed in aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerland) in pigs. forty-eight individually housed pigs (landrace × pietrain; 52 kg bw; 24 males and 24 females) were distributed among 6 experimental treatments consisting of a low-p diet (3.5 g p/kg; 1.1 g digestible p/kg), which was supplemented with 500, 1000, 2000, or 4000 units of phytase activity/kg, and a standard-p diet ...201223365288
synthetic dye decolorization by three sources of fungal laccase.decolorization of six synthetic dyes using three sources of fungal laccase with the origin of aspergillus oryzae, trametes versicolor, and paraconiothyrium variabile was investigated. among them, the enzyme from p. variabile was the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-s (56%), rimazol brilliant blue r (rbbr; 47%), congo red (18.5%), and methylene blue (21.3%) after 3 h incubation in presence of hydroxybenzotriazole (hbt; 5 mm) as the l ...201223369690
enzymatic synthesis of 2-aminoethyl β-d-galactopyranoside catalyzed by aspergillus oryzae β-galactosidase.glycosidases provide a powerful resource for in vitro synthesis of novel anomerically pure glycosides. generation of new low molecular weight galactosides is of interest since they are potential galectin inhibitors. galectins are molecular targets for cancer therapy and thus their inhibitors are potential antitumor agents. here we report the enzymatic synthesis and structural characterization of 2-aminoethyl β-d-galactopyranoside. critical parameters for transgalactosylation using either soluble ...201223357285
a straightforward access to tmg-chitooligomycins and their evaluation as β-n-acetylhexosaminidase inhibitors.a chemo-biotechnological approach is reported for the synthesis of tmg-chitooligomycins, co-n (nme(3)). their abilities to inhibit β-n-acetylhexosaminidases (hexnacases), from aspergillus oryzae (aohex, fungi), canavalia ensiformis (cehex, plant) hexnacases and a chitobiase from serratia marcescens (smchb, bacteria) were studied and compared with their precursors co-n (n). co-n (nme(3)) were revealed as potent inhibitors for aohex and smchb with a proved chain length effect while co-n (n) was a ...201223333949
identification of csypyrone b2 and b3 as the minor products of aspergillus oryzae type iii polyketide synthase csyb.since our first report on the identification of the fungal type iii polyketide synthase (pks) genes csya~d in aspergillus oryzae rib40, type iii pks homologues have also been found in other fungal species. we previously reported the isolation and structural determination of csypyrone b1 as the main product of csyb when inductively expressed in aspergillus oryzae. herein we report the isolation and identification of the two minor products of the csyb transformant in addition to csypyrone b1 as 4- ...201223290452
lethal and mutagenic effects of ion beams and γ-rays in aspergillus oryzae.aspergillus oryzae is a fungus that is used widely in traditional japanese fermentation industries. in this study, the lethal and mutagenic effects of different linear energy transfer (let) radiation in freeze-dried conidia of a. oryzae were investigated. the lethal effect, which was evaluated by a 90% lethal dose, was dependent on the let value of the ionizing radiation. the most lethal ionizing radiation among that tested was (12)c(5+) ion beams with an let of 121kev/μm. the (12)c(5+) ion beam ...201223280012
in vitro antifungal activity of dehydrozingerone and its fungitoxic properties.the efficacy of dehydrozingerone (dz; dehydroderivative of zingerone) as an antifungal agent and its mode of action against food spoilage fungal pathogens was studied and presented. dz is a constituent of ginger (zingiber officinale rhizomes) and structural half analogue of curcumin. its efficacy against aspergillus oryzae, aspergillus flavus, aspergillus niger, aspergillus ochraceus, fusarium oxysporum and penicillium chrysogenum was evaluated. effect of dz on the growth and sporulation of a. o ...201223278709
assessing the genome-wide effect of promoter region tandem repeat natural variation on gene expression.copy number polymorphisms of nucleotide tandem repeat (tr) regions, such as microsatellites and minisatellites, are mutationally reversible and highly abundant in eukaryotic genomes. studies linking tr polymorphism to phenotypic variation have led some to suggest that tr variation modulates and majorly contributes to phenotypic variation; however, studies in which the authors assess the genome-wide impact of tr variation on phenotype are lacking. to address this question, we quantified relations ...201223275886
an integrative process model of enzymatic biodiesel production through ethanol fermentation of brown rice followed by lipase-catalyzed ethanolysis in a water-containing system.we attempted to integrate lipase-catalyzed ethanolysis into fermentative bioethanol production. to produce bioethanol, ethanol fermentation from brown rice was conducted using a tetraploid saccharomyces cerevisiae expressing α-amylase and glucoamylase. the resultant ethanol was distilled and separated into three fractions with different concentrations of water and fusel alcohols. in ethanolysis using the first fraction with 89.3% ethanol, a recombinant aspergillus oryzae whole-cell biocatalyst e ...201223273281
structural basis for the formation of acylalkylpyrones from two β-ketoacyl units by the fungal type iii polyketide synthase csyb.the acylalkylpyrone synthase csyb from aspergillus oryzae catalyzes the one-pot formation of the 3-acyl-4-hydroxy-6-alkyl-α-pyrone scaffold from acetoacetyl-coa, fatty acyl-coa, and malonyl-coa. this is the first type iii polyketide synthase that performs not only the polyketide chain elongation but also the condensation of two β-ketoacyl units. the crystal structures of wild-type csyb and its i375f and i375w mutants were solved at 1.7-, 2.3-, and 2.0-å resolutions, respectively. the crystal str ...201525564614
phylogenetic and structural analysis of polyketide synthases in aspergilli.polyketide synthases (pkss) of aspergillus species are multidomain and multifunctional megaenzymes that play an important role in the synthesis of diverse polyketide compounds. putative pks protein sequences from aspergillus species representing medically, agriculturally, and industrially important aspergillus species were chosen and screened for in silico studies. six candidate aspergillus species, aspergillus fumigatus af293, aspergillus flavus nrrl3357, aspergillus niger cbs 513.88, aspergill ...201627199544
effect of heavy atoms on the thermal stability of α-amylase from aspergillus oryzae.currently, there are no versatile and established methods for improving stability of proteins. in an entirely different approach from conventional techniques such as mutagenesis, we attempted to enhance enzyme stability of α-amylase from aspergillus oryzae using a heavy-atom derivatization technique. we evaluated changes in stability using differential scanning calorimetry (dsc). candidate heavy atoms were identified using the heavy-atom database system hatodas, a web-based tool designed to assi ...201323451229
immobilization, regiospecificity characterization and application of aspergillus oryzae lipase in the enzymatic synthesis of the structured lipid 1,3-dioleoyl-2-palmitoylglycerol.the enzymatic synthesis of 1,3-dioleoyl-2-palmitoylglycerol (opo), one of the main components of human milk fats, has been hindered by the relatively high cost of sn-1,3-specific lipases and the deficiency in biocatalyst stability. the sn-1,3-specific lipase from aspergillus oryzae (aol) is highly and efficiently immobilized with the polystyrene-based hydrophobic resin d3520, with a significant 49.54-fold increase in specific lipase activity compared with the aol powder in catalyzing the synthes ...201526218640
entry inhibition of influenza viruses with high mannose binding lectin esa-2 from the red alga eucheuma serra through the recognition of viral hemagglutinin.lectin sensitivity of the recent pandemic influenza a virus (h1n1-2009) was screened for 12 lectins with various carbohydrate specificity by a neutral red dye uptake assay with mdck cells. among them, a high mannose (hm)-binding anti-hiv lectin, esa-2 from the red alga eucheuma serra, showed the highest inhibition against infection with an ec50 of 12.4 nm. moreover, esa-2 exhibited a wide range of antiviral spectrum against various influenza strains with ec50s of pico molar to low nanomolar leve ...201526035023
co-metabolic degradation of β-cypermethrin and 3-phenoxybenzoic acid by co-culture of bacillus licheniformis b-1 and aspergillus oryzae m-4.the degradation efficiency of organic contaminants and their associated metabolites by co-culture of microbes is mainly limited by toxic intermediates from co-metabolic degradation. in this study, we investigated the degradation of β-cypermethrin (β-cy) and 3-phenoxybenzoic acid (3-pba) by co-culture of bacillus licheniformis b-1 and aspergillus oryzae m-4, as well as the influences of β-cy and 3-pba metabolites on their degradation and the growth of strains b-1 and m-4. our results indicated th ...201627898684
structural and catalytic properties of s1 nuclease from aspergillus oryzae responsible for substrate recognition, cleavage, non-specificity, and inhibition.the single-strand-specific s1 nuclease from aspergillus oryzae is an archetypal enzyme of the s1-p1 family of nucleases with a widespread use for biochemical analyses of nucleic acids. we present the first x-ray structure of this nuclease along with a thorough analysis of the reaction and inhibition mechanisms and of its properties responsible for identification and binding of ligands. seven structures of s1 nuclease, six of which are complexes with products and inhibitors, and characterization ...201628036383
separation and characterization of the immunostimulatory components in unpolished rice black vinegar (kurozu).unpolished rice black vinegar (kurozu), a traditional japanese vinegar, is considered to have beneficial health effects. kurozu is produced via a static fermentation process involving the saccharification of rice by aspergillus oryzae, alcohol fermentation by saccharomyces cerevisiae, and the oxidation of ethanol to acetic acid by acetic acid bacteria such as acetobacter pasteurianus. since this process requires about 6 months' fermentation and then over a year of aging, most of these organisms ...201323810669
dietary supplementation with bacillus subtilis, saccharomyces cerevisiae and aspergillus oryzae enhance immunity and disease resistance against aeromonas hydrophila and streptococcus iniae infection in juvenile tilapia oreochromis niloticus.a feeding trial was conducted to investigate the effects of dietary administration of probiotic with bacillus subtilis, aspergillus oryzae and saccharomyces cerevisiae on growth, innate immune response, hemato-immunological parameters and disease resistance of nile tilapia, oreochromis niloticus. animals were distributed in three equal groups, each of five replicates and received one of the following experimental diets for four weeks: control, non-supplemented diet; 5 g kg(-1) probiotic mixture ...201525530581
the construction and use of versatile binary vectors carrying pyrg auxotrophic marker and fluorescent reporter genes for agrobacterium-mediated transformation of aspergillus oryzae.aspergillus oryzae is a safe mold widely used in food industry. it is also considered as a microbial cell factory for production of recombinant proteins and enzymes. currently, genetic manipulation of filamentous fungi is achieved via agrobacterium tumefaciens-mediated transformation methods usually employing antibiotic resistance markers. these methods are hardly usable for a. oryzae due to its strong resistance to the common antifungal compounds used for fungal transformation. in this study, w ...201627804102
deletion of afpab1 causes increased sensitivity to oxidative stress and hypovirulence in aspergillus fumigatus.aspergillus fumigatus afpab1 is the ortholog of the aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules aopab1 that function to depress the initiation of translation during stress. a. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on pab1 in a. fumigatus. the associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain δafpab1. phenotypic analysis showed that the δafpab1 grew m ...201627801871
ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease.the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ...201526417353
nicotine-degrading microorganisms and their potential applications.nicotine-degrading microorganisms (ndms) are a special microbial group which can use nicotine as the sole carbon and nitrogen source for growth. since the 1950s, the bioconversion of nicotine by microbes has received increasing attention, and several ndms have been identified, such as arthrobacter nicotinovorans, microsporum gypseum, pellicularia filamentosa jts-208, and pseudomonas sp. 41. in recent years, increasing numbers of ndms have been isolated and identified from tobacco plantation soil ...201525805341
biodegradation of nicotine by a novel nicotine-degrading bacterium, pseudomonas plecoglossicida tnd35 and its new biotransformation intermediates.tobacco wastes that contain nicotine alkaloids are harmful to human health and the environment. in the investigation, a novel nicotine-biodegrading bacterium tnd35 was isolated and identified as pseudomonas plecoglossicida on the basis of phenotypic, biochemical characteristics and 16s rrna sequence homology. we have studied the nicotine biodegradation potential of strain tnd35 by detecting the intermediate metabolites using an array of approaches such as hplc, gc-ms, nmr and ft-ir. biotransform ...201423604517
conjugation of deoxynivalenol by alternaria alternata (54028 nrrl), rhizopus microsporus var. rhizopodiformis (54029 nrrl) and aspergillus oryzae (5509 nrrl).deoxynivalenol (don, vomitoxin) is a trichothecene mycotoxin which can be considered to be an indicator of fusarium mycotoxin contamination in grain, feed and food. recent studies have described the presence of glucose conjugated don, which is a product of plant metabolism, but there is a lack of information available on don conjugation by fungi. the aim of the current study was, therefore, to investigate the ability of fungi to metabolize don into hydrolysable conjugated don. alternaria alterna ...201424263850
transformation of litchi pericarp-derived condensed tannin with aspergillus awamori.condensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. in this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging activity of the extracted polyphenol after litchi pericarp is treated with aspergillus awamori, aspergillus sojae or aspergillus oryzae. we have further explored the activity of a. awamori in the formation of condensed tannin. the treatment of a. awamori appeared to produce ...201627420043
biohydrogen production in the suspended and attached microbial growth systems from waste pastry hydrolysate.waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then, the effects of hydraulic retention times (hrts) (4-12h) on hydrogen production rate (hpr) in the suspended microbial growth system (continuous stirred tank reactor, cstr) and attached microbial growth system (continuous mixed immobilized sludge reactor, cmisr) from waste pastry hydrolysate were investig ...201627416509
valorization of bakery waste for biocolorant and enzyme production by monascus purpureus.a concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by monasucus purpureus has been developed. the proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by hong kong or other countries. bakery waste collected from bakery store was used in submerged and solid-state fermentation of monascus purpureus to produce bio-colorant, which could potentially be applied in food and text ...201627208753
continuous biohydrogen production from waste bread by anaerobic this study, continuous biohydrogen production from waste bread by anaerobic sludge was performed. the waste bread was first hydrolyzed by the crude enzymes which were generated by aspergillus awamori and aspergillus oryzae via solid-state fermentation. it was observed that 49.78g/l glucose and 284.12mg/l free amino nitrogen could be produced with waste bread mass ratio of 15% (w/v). the waste bread hydrolysate was then used for biohydrogen production by anaerobic sludge in a continuous stirre ...201627065225
valorisation of mixed bakery waste in non-sterilized fermentation for l-lactic acid production by an evolved thermoanaerobacterium sp. this study, an advanced biorefinery technology that uses mixed bakery waste has been developed to produce l-lactic acid using an adaptively evolved thermoanaerobacterium aotearoense la1002-g40 in a non-sterilized system. under these conditions, mixed bakery waste was directly hydrolysed by aspergillus awamori and aspergillus oryzae, resulting in a nutrient-rich hydrolysate containing 83.6g/l glucose, 9.5 g/l fructose and 612 mg/l free amino nitrogen. t. aotearoense la1002-g40 was evaluated an ...201526363501
batch dark fermentation from enzymatic hydrolyzed food waste for hydrogen production.a combination bioprocess of solid-state fermentation (ssf) and dark fermentative hydrogen production from food waste was developed. aspergillus awamori and aspergillus oryzae were utilized in ssf from food waste to generate glucoamylase and protease which were used to hydrolyze the food waste suspension to get the nutrients-rich (glucose and free amino nitrogen (fan)) hydrolysate. both glucose and fan increased with increasing of food waste mass ratio from 4% to 10% (w/v) and the highest glucose ...201525978853
mixed food waste as renewable feedstock in succinic acid fermentation.mixed food waste, which was directly collected from restaurants without pretreatments, was used as a valuable feedstock in succinic acid (sa) fermentation in the present study. commercial enzymes and crude enzymes produced from aspergillus awamori and aspergillus oryzae were separately used in hydrolysis of food waste, and their resultant hydrolysates were evaluated. for hydrolysis using the fungal mixture comprising a. awamori and a. oryzae, a nutrient-complete food waste hydrolysate was genera ...201425149459
structural analysis of cerebrosides from aspergillus fungi: the existence of galactosylceramide in a. oryzae.glucosylceramide and galactosylceramide were detected in three aspergillus species: aspergillus oryzae, aspergillus sojae and aspergillus. awamori, using borate-coated tlc. the cerebrosides from a. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determined the composition of sugar, fatty acid and sphingoid base by gc/ms, maldi-tof/ms and (1)h-nmr. we identified them as β-glucosylceramide and β-galactosylceramide. the ceramide moiety ...201425129050
upgrading the antioxidant potential of cereals by their fungal fermentation under solid-state cultivation conditions.solid-state fermentation (ssf) at 30°c for 72 h with four generally recognized as safe (gras) filamentous fungi (aspergillus oryzae ncim 1212, aspergillus awamori mtcc no. 548, rhizopus oligosporus ncim 1215 and rhizopus oryzae rck2012) showed high efficiency for the improvement of water-soluble total phenolic content (tpc) and antioxidant properties including abts(●+) [2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid)] and dpph(●) (2,2'-diphenyl-1-picrylhydrazyl) scavenging capacities of ...201424964826
fungal hydrolysis in submerged fermentation for food waste treatment and fermentation feedstock preparation.potential of fungal hydrolysis in submerged fermentation by aspergillus awamori and aspergillus oryzae as a food waste treatment process and for preparation of fermentation feedstock has been investigated. by fungal hydrolysis, 80-90% of the initial amount of waste was reduced and degraded within 36-48 h into glucose, free amino nitrogen (fan) and phosphate. experiments revealed that 80-90% of starch can be converted into glucose and highest concentration of fan obtained, when solid mashes of a. ...201424583214
food waste as nutrient source in heterotrophic microalgae cultivation.glucose, free amino nitrogen (fan), and phosphate were recovered from food waste by fungal hydrolysis using aspergillus awamori and aspergillus oryzae. using 100g food waste (dry weight), 31.9 g glucose, 0.28 g fan, and 0.38 g phosphate were recovered after 24h of hydrolysis. the pure hydrolysate has then been used as culture medium and nutrient source for the two heterotrophic microalgae schizochytrium mangrovei and chlorella pyrenoidosa, s. mangrovei and c. pyrenoidosa grew well on the complex ...201323587816
analyzing the relation between the microbial diversity of daqu and the turbidity spoilage of traditional chinese vinegar.vinegar is a traditional fermented condiment, and the microbial diversity of daqu makes the quality of vinegar products. recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. in this study, the relation between the microbial diversity of daqu and turbidity spoilage of vinegar was analyzed by plating technique, pcr-denaturing gradient gel electrophoresis (dgge), and high-performance liquid chromatography (hplc). the 16s rrna sequencing and dgge analysis indicated t ...201424691870
identification of ophiobolin f synthase by a genome mining approach: a sesterterpene synthase from aspergillus clavatus.during a screening of putative diterpene synthase genes found in public databases using the aspergillus oryzae expression system, it was found that a single transformant with the acla_76850 gene from a. clavatus produced a sesterterpene alcohol, ophiobolin f, and three minor sesterterpene hydrocarbons. the sesterterpene synthase has two catalytically independent domains (prenyltransferase/terpene cyclase) which are homologous to those of diterpene synthase, fusicoccadiene synthase. coevolution o ...201323324037
genome mining for sesterterpenes using bifunctional terpene synthases reveals a unified intermediate of di/sesterterpenes.genome mining is a promising method to discover novel secondary metabolites in the postgenomic era. we applied the aspergillus oryzae heterologous expression system to functionally characterize cryptic bifunctional terpene synthase genes found in fungal genomes and identified the sesterfisherol synthase gene (nfss) from neosartorya fischeri. sesterfisherol contains a characteristic 5-6-8-5 tetracyclic ring system and is modified by cytochrome p450 monooxygenase (nfp450) to sesterfisheric acid. t ...201526332841
a promiscuous prenyltransferase from aspergillus oryzae catalyses c-prenylations of hydroxynaphthalenes in the presence of different prenyl donors.prenyltransferases of the dimethylallyltryptophan synthase (dmats) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. most members of this superfamily accepted as prenyl donor exclusively dimethylallyl diphosphate (dmapp). one enzyme, anapt from neosartorya fischeri, was reported recently to use both dmapp and geranyl diphosphate (gpp) as prenyl do ...201424430210
novel inexpensive fungi proteases: production by solid state fermentation and characterization.a comparative study was carried out for proteases production using agroindustrial residues as substrate for solid state fermentation (ssf) of several fungal strains. high protease production was observed for most of the microorganisms studied, as well as very different biochemical characteristics, including activities at specific temperatures and a wide range of ph values. the enzymes produced were very different regarding optimum ph and they showed stability at 50 °c. aspergillus oryzae showed ...201626769513
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens.filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear.201728100314
invasive forms of candida and aspergillus in sputum samples of pulmonary tuberculosis patients attending the tuberculosis reference laboratory in ghaemshahr, northern iran: an analysis of samples collected during the past 10years.tuberculosis (tb) continues to be a major public health problem worldwide. this is especially true in northern iran, which has high tb prevalence. the chronic nature of this disease is further exacerbated if it is accompanied by fungal infection, which usually remains undiagnosed and thus untreated. thus, mycotic infections add fatal dimensions to pulmonary tb. our objective was to determine the prevalence of invasive forms of fungal elements in sputum samples collected from patients with pulmon ...201628043539
fungal elicitors enhance ginsenosides biosynthesis, expression of functional genes as well as signal molecules accumulation in adventitious roots of panax ginseng c. a. this work, we selected three fungi strains (aspergillus niger, aspergillus flavus and aspergillus oryzae) as elicitors prepared from mycelium or fermentation broth to improve ginsenosides production in adventitious roots culture. the results showed that ginsenosides production (29.90±4.67mgg(-1)) was significantly enhanced upon elicitation with 200mgl(-1)a. niger elicitor prepared from mycelium, which was 3.52-fold of untreated group. hplc-esi-ms(n) analysis was performed, showing that ginsen ...201627746309
expression of ustr and the golgi protease kexb are required for ustiloxin b biosynthesis in aspergillus oryzae.ustiloxin b, originally isolated from the fungus ustilaginoidea virens, is a known inhibitor of microtubule assembly. ustiloxin b is also produced by aspergillus flavus and is synthesized through the ribosomal peptide synthesis pathway. in a. flavus, the gene cluster associated with ustiloxin b production contains 15 genes including those encoding a fungal c6-type transcription factor and ustiloxin b precursor. although the koji mold aspergillus oryzae, which is genetically close to a. flavus, h ...201626842395
[isolation and study on the aflatoxin genes of aflatoxin-producing fungi in paprika samples in chengdu].to isolate aflatoxin-producing strains from paprika samples and to do a preliminarily study on the relationship between aflatoxin-producing ability and the genes aflr, omt-1 and ver-1.201526619535
distinguishing the dominant species of pathogen in maxillary sinusitis by sequencing dna dataset analysis.this study determined the microbial composition in maxillary sinusitis and determined the predominant fungal and bacterial species. samples were obtained from 11 patients with fungal sinusitis and 2 healthy patients. the 13 samples were sequenced using an illumina hi-seq 2000, and mapping to the human, bacterial, and the fungal genomes were based on several steps. bioinformatics and statistical analyses were then performed for bacterial and fungal expressions in the samples. all samples were cul ...201525724392
high sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of aspergillus oryzae.aspergillus oryzae and aspergillus flavus are closely related fungal species. the a. flavus morphotype that produces numerous small sclerotia (s strain) and aflatoxin has a unique 1.5 kb deletion in the norb-cypa region of the aflatoxin gene cluster (i.e. the s genotype). phylogenetic studies have indicated that an isolate of the nonaflatoxigenic a. flavus with the s genotype is the ancestor of a. oryzae. genome sequence comparison between a. flavus nrrl3357, which produces large sclerotia (l st ...201525689355
an evaluation of aflatoxin and cyclopiazonic acid production in aspergillus date, edible fungi such as aspergillus flavus var. oryzae (a. oryzae) has been considered as safe. however, some strains can produce mycotoxins. thus, the biosynthetic ability to produce mycotoxins should be reevaluated to determine the safety of edible fungi. we analyzed the production of aflatoxins and cyclopiazonic acid (cpa) from edible fungi such as a. oryzae isolated from various korean foods using multiplex pcr, enzyme-linked immunosorbent assay, and high-performance liquid chromatogra ...201424853527
non-aflatoxigenicity of commercial aspergillus oryzae strains due to genetic defects compared to aflatoxigenic aspergillus flavus.aspergillus oryzae is generally recognized as safe, but it is closely related to a. flavus in morphology and genetic characteristics. in this study, we tested the aflatoxigenicity and genetic analysis of nine commercial a. oryzae strains that were used in korean soybean fermented products. cultural and hplc analyses showed that none of the commercial strains produced detectable amount of aflatoxins. according to the molecular analysis of 17 genes in the aflatoxin (af) biosynthetic pathway, the c ...201424759426
drosera peltata smith var. lunata (buch.-ham.) c. b. clarke as a feasible source of plumbagin: phytochemical analysis and antifungal activity assay.drosera peltata smith var. lunata (buch.-ham.) c. b. clarke (dpvl) fractions and plumbagin were tested via broth microdilution techniques on rhizopus oryzae, aspergillus flavus, aspergillus niger, aspergillus oryzae, penicillium citrinum. all of the test substances [petroleum ether, chloroform, ethyl acetate, n-butanol fraction and aqueous residue (ar)] except for the ar were active against all the tested strains. the petroleum ether fraction (pef) was the most active (mic = 5.86-46.88 μg/ml, mf ...201424078108
investigation of malic acid production in aspergillus oryzae under nitrogen starvation conditions.malic acid has great potential for replacing petrochemical building blocks in the future. for this application, high yields, rates, and titers are essential in order to sustain a viable biotechnological production process. natural high-capacity malic acid producers like the malic acid producer aspergillus flavus have so far been disqualified because of special growth requirements or the production of mycotoxins. as a. oryzae is a very close relative or even an ecotype of a. flavus, it is likely ...201323892740
antifungal effect of allium tuberosum, cinnamomum cassia, and pogostemon cablin essential oils and their components against population of aspergillus species.antifungal activity of allium tuberosum (at), cinnamomum cassia (cc), and pogostemon cablin (patchouli, p) essential oils against aspergillus flavus strains 3.2758 and 3.4408 and aspergillus oryzae was tested at 2 water activity levels (aw : 0.95 and 0.98). main components of tested essential oils were: allyl trisulfide 40.05% (at), cinnamaldehyde 87.23% (cc), and patchouli alcohol 44.52% (p). the minimal inhibitory concentration of the plant essential oils against a. flavus strains 3.2758 and 3 ...201323647469
isolation and characterization of a proteinaceous antifungal compound from lactobacillus plantarum yml007 and its application as a food preservative.korean kimchi is known for its myriad of lactic acid bacteria (lab) with diverse bioactive compounds. this study was undertaken to isolate an efficient antifungal lab strain among the isolated kimchi labs. one thousand and four hundred labs isolated from different kimchi samples were initially screened against aspergillus niger. the strain exhibiting the highest antifungal activity was identified as lactobacillus plantarum yml007 by 16s rrna sequencing and biochemical assays using api 50 chl kit ...201323565693
synthesis of novel 2-amino-4-(5'-substituted 2'-phenyl-1h-indol-3'-yl)-6-aryl-4h-pyran-3-carbonitrile derivatives as antimicrobial and antioxidant a part of systematic investigation of synthesis and biological activities of indole analogues linked to various heterocyclic systems, we have synthesized new compounds viz., 2-amino-4-(5'-substituted 2'-phenyl-1h-indol-3'-yl)-6-aryl-4h-pyran-3-carbonitriles (2a-i), 4,5-diamino-6-(5'-substituted 2'-phenyl-1h-indol-3'-yl)-8-aryl-2-oxo-2,6-dihydrodipyrano [2,3-b:3,2-e]pyridine-3-carbonitriles (3a-i), 4-amino-5-(5'-substituted 2'-phenyl-1h-indol-3-yl)-7-aryl-1h-pyrano[2,3-d]pyrimidin-2(5h)-ones ( ...201323454016
evaluation of multiplexed pcr and liquid-phase array for identification of respiratory fungal pathogens.invasive fungal infections are the cause of serious morbidity and high mortality in immunocompromised patients. early laboratory diagnostic options remain limited; however, rapid detection and accurate identification may improve outcome. herein, multiplexed pcr followed by liquid-phase array was evaluated for detection and identification of common respiratory fungal pathogens, including aspergillus fumigatus, rhizopus microsporus, scedosporium apiospermum and fusarium solani. the limit of detect ...201222435876
gliotoxin effects on fungal growth: mechanisms and exploitation.although initially investigated for its antifungal properties, little is actually known about the effect of gliotoxin on aspergillus fumigatus and other fungi. we have observed that exposure of a. fumigatus to exogenous gliotoxin (14 μg/ml), under gliotoxin-limited growth conditions, results in significant alteration of the expression of 27 proteins (up- and down-regulated >1.9-fold; p<0.05) including de novo expression of cu, zn superoxide dismutase, up-regulated allergen asp f3 expression and ...201222405895
regulation of development in aspergillus nidulans and aspergillus fumigatus.members of the genus aspergillus are the most common fungi and all reproduce asexually by forming long chains of conidiospores (or conidia). the impact of various aspergillus species on humans ranges from beneficial to harmful. for example, several species including aspergillus oryzae and aspergillus niger are used in industry for enzyme production and food processing. in contrast, aspergillus flavus produce the most potent naturally present carcinogen aflatoxins, which contaminate various plant ...201023956662
a novel zn2-cys6 transcription factor atrr plays a key role in an azole resistance mechanism of aspergillus fumigatus by co-regulating cyp51a and cdr1b expressions.successful treatment of aspergillosis caused by aspergillus fumigatus is threatened by an increasing incidence of drug resistance. this situation is further complicated by the finding that strains resistant to azoles, the major antifungal drugs for aspergillosis, have been widely disseminated across the globe. to elucidate mechanisms underlying azole resistance, we identified a novel transcription factor that is required for normal azole resistance in aspergillus fungi including a. fumigatus, as ...201728052140
comparative transcriptome analysis revealing dormant conidia and germination associated genes in aspergillus species: an essential role for atfa in conidial dormancy.fungal conidia are usually dormant unless the extracellular conditions are right for germination. despite the importance of dormancy, little is known about the molecular mechanism underlying entry to, maintenance of, and exit from dormancy. to gain comprehensive and inter-species insights, transcriptome analyses were conducted across aspergillus fumigatus, aspergillus niger, and aspergillus oryzae.201627185182
three extracellular dipeptidyl peptidases found in aspergillus oryzae show varying substrate specificities.three extracellular dipeptidyl peptidase genes, dppb, dppe, and dppf, were unveiled by sequence analysis of the aspergillus oryzae genome. we investigated their differential enzymatic profiles, in order to gain an understanding of the diversity of these genes. the three dipeptidyl peptidases were expressed using aspergillus nidulans as the host. each recombinant enzyme was purified and subsequently characterized. the enzymes displayed similar optimum ph values, but optimum temperatures, ph stabi ...201626846741
the mycobiota of air inside and outside the meju fermentation room and the origin of meju fungi.the fungi on meju are known to play an important role as degrader of macromolecule of soybeans. in order to elucidate the origin of fungi on traditional meju, mycobiota of the air both inside and outside traditional meju fermentation rooms was examined. from 11 samples of air collected from inside and outside of 7 meju fermentation rooms, 37 genera and 90 species of fungi were identified. in outside air of the fermentation room, cladosporium sp. and cladosporium cladosporioides were the dominant ...201526539041
production and characterization of two major aspergillus oryzae secreted prolyl endopeptidases able to efficiently digest proline-rich peptides of gliadin.prolyl endopeptidases are key enzymes in the digestion of proline-rich proteins. fungal extracts rich in prolyl endopeptidases produced by a species such as aspergillus oryzae used in food fermentation would be of particular interest for the development of an oral enzyme therapy product in patients affected by intolerance to gluten. two major a. oryzae secreted prolyl endopeptidases of the merops s28 peptidase family, aos28a and aos28b, were identified when this fungus was grown at acidic ph in ...201526464108
conserved and varied dinucleotide sequences in the genomes of three aspergillus species.both guanine-cytosine content and nucleosome occupancy are higher in exons than in introns. in this study, the association between the frequencies of the dinucleotide sequences and the nucleosome occupancy of the exons and introns of the genes of aspergillus fumigatus, aspergillus nidulans, and aspergillus oryzae was studied. the frequency of the dinucleotide sequences aa (tt), at, and ta in the introns was more than that in the exons. in addition, the frequency of these sequences in the regions ...201425564023
the aspergillus genome database: multispecies curation and incorporation of rna-seq data to improve structural gene annotations.the aspergillus genome database (aspgd; is a freely available web-based resource that was designed for aspergillus researchers and is also a valuable source of information for the entire fungal research community. in addition to being a repository and central point of access to genome, transcriptome and polymorphism data, aspgd hosts a comprehensive comparative genomics toolbox that facilitates the exploration of precomputed orthologs among the 20 currently available asperg ...201424194595
comparative genome analysis between aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among aspergillus species.aspergillus oryzae has been utilized for over 1000 years in japan for the production of various traditional foods, and a large number of a. oryzae strains have been isolated and/or selected for the effective fermentation of food ingredients. characteristics of genetic alterations among the strains used are of particular interest in studies of a. oryzae. here, we have sequenced the whole genome of an industrial fungal isolate, a. oryzae rib326, by using a next-generation sequencing system and com ...201222912434
a comparative study of hydrolysis and transglycosylation activities of fungal β-glucosidases.β-glucosidases (bgs) from aspergillus fumigatus, aspergillus niger, aspergillus oryzae, magnaporthe grisea, neurospora crassa, and penicillium brasilianum were purified to homogeneity, and investigated for their (simultaneous) hydrolytic and transglycosylation activity in samples with high concentrations of either cellobiose or glucose. the rate of the hydrolytic process (which converts one cellobiose to two glucose molecules) shows a maximum around 10-15 mm cellobiose and decreases with further ...201322311644
self-excising cre/mutant lox marker recycling system for multiple gene integrations and consecutive gene deletions in aspergillus this study, we developed a self-excising cre/loxp-mediated marker recycling system with mutated lox sequences to introduce a number of biosynthetic genes into aspergillus oryzae. to construct the self-excising marker cassette, both the selectable marker, the aspergillus nidulans adea gene, and the cre recombinase gene (cre), conditionally expressed by the xylanase-encoding gene promoter, were designed to be located between the mutant lox sequences, lox66 and lox71. however, construction of th ...201728011085
analysis of the ionic interaction between the hydrophobin roda and two cutinases of aspergillus nidulans obtained via an aspergillus oryzae expression system.hydrophobins are amphipathic secretory proteins with eight conserved cysteine residues and are ubiquitous among filamentous fungi. in the fungus aspergillus oryzae, the hydrophobin rola and the polyesterase cutl1 are co-expressed when the sole available carbon source is the biodegradable polyester polybutylene succinate-co-adipate (pbsa). rola promotes the degradation of pbsa by attaching to the particle surface, changing its structure and interacting with cutl1 to concentrate cutl1 on the pbsa ...201727917435
increased enzyme production under liquid culture conditions in the industrial fungus aspergillus oryzae by disruption of the genes encoding cell wall α-1,3-glucan synthase.under liquid culture conditions, the hyphae of filamentous fungi aggregate to form pellets, which reduces cell density and fermentation productivity. previously, we found that loss of α-1,3-glucan in the cell wall of the fungus aspergillus nidulans increased hyphal dispersion. therefore, here we constructed a mutant of the industrial fungus a. oryzae in which the three genes encoding α-1,3-glucan synthase were disrupted (tripleδ). although the hyphae of the tripleδ mutant were not fully disperse ...201627442340
involvement of an srf-mads protein mcma in regulation of extracellular enzyme production and asexual/sexual development in aspergillus nidulans.srf-mads proteins are transcription factors conserved among eukaryotes that regulate a variety of cellular functions; however, their physiological roles are still not well understood in filamentous fungi. effects of a mutation in mcma gene that encodes the sole srf-mads protein in the fungus aspergillus nidulans were examined by rna sequencing. sequencing data revealed that expression levels of cellulase genes were significantly decreased by the mutation as reported previously. however, expressi ...201626967516
cytotoxicity and colloidal behavior of polystyrene latex nanoparticles toward filamentous fungi in isotonic solutions.the effects of surface physicochemical properties of functionalized polystyrene latex (psl) nanoparticles (nps) and model filamentous fungi aspergillus oryzae and aspergillus nidulans cultivated in different environment (aqueous and atmospheric environment) on the colloidal behavior and cytotoxicity were investigated in different isotonic solutions (154 mm nacl and 292 mm sucrose). when the liquid cultivated fungal cells were exposed to positively charged psl nps in 154 mm nacl solution, the nps ...201626855210
soil fungi for mycoremediation of arsenic pollution in agriculture soils.soil arsenic (as) contamination of food-chains and public health can be mitigated through fungal bioremediation. to enumerate culturable soil fungi, soils were collected from the as-contaminated paddy fields (3-35 mg kg(-1) ) of the middle indo-gangetic plains.201526348882
insecticidal activity of ethyl acetate extracts from culture filtrates of mangrove fungal the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. toxicity tests of all extracts against brine shrimp (artemia salina) larvae were performed. the extracts with the highest toxicity were further examined for insecticidal activity against spodoptera litura larvae and acetylcholinesterase (ache) inhibition activity. the results showed th ...201526190921
an overproduction of astellolides induced by genetic disruption of chromatin-remodeling factors in aspergillus oryzae.the filamentous fungus aspergillus oryzae is an important industrial mold. recent genomic analysis indicated that a. oryzae has a large number of biosynthetic genes for secondary metabolites (sms), but many of the sms they produce have not been identified. for better understanding of sms production by a. oryzae, we screened a gene-disruption library of transcription factors including chromatin-remodeling factors and found two gene disruptions that show similarly altered sm production profiles. o ...201626126743
construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in aspergillus oryzae.the filamentous fungus aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. to facilitate higher yields of proteins of interest, we constructed the pasop vector to express heterologous proteins in a. oryzae. pasop carries a selectable marker, pyrg, derived from aspergillus nidulans, and a strong promoter and a terminator of the amyb gene derived from a. oryzae. pasop transformed a. oryzae efficiently via t ...201525674804
increased production of free fatty acids in aspergillus oryzae by disruption of a predicted acyl-coa synthetase gene.fatty acids are attractive molecules as source materials for the production of biodiesel fuel. previously, we attained a 2.4-fold increase in fatty acid production by increasing the expression of fatty acid synthesis-related genes in aspergillus oryzae. in this study, we achieved an additional increase in the production of fatty acids by disrupting a predicted acyl-coa synthetase gene in a. oryzae. the a. oryzae genome is predicted to encode six acyl-coa synthetase genes and disruption of ao0900 ...201525564035
Displaying items 1801 - 1900 of 2435